Search

Your search keyword '"Lu, Qingjun"' showing total 133 results
Start Over Author "Lu, Qingjun"
133 results on '"Lu, Qingjun"'

5. A new contribution for an old drug: Prospect of metformin in colorectal oncotherapy

Author

Jiang, Shangze and Lu, Qingjun

Subjects
Drug discovery, Colorectal cancer -- Drug therapy, Metformin -- Usage, Health
Abstract

Byline: Shangze. Jiang, Qingjun. Lu Metformin is well known as a hypoglycemic drug, which maintains glucose blood balance by attenuating hepatic glycogen synthesis and enhancing muscle glucose decomposition. The accumulation [...]

Published
2021

17. Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes

Author

Lu Qingjun, Jingxue Zhang, Ningli Wang, Wu Liu, Meng Gao, Qian Liu, Liang Xu, Jinqiu Chen, and Shen Wu

Subjects
0301 basic medicine, Vascular Endothelial Growth Factor A, Mechanical stretch, medicine.medical_treatment, Cell, Blotting, Western, Apoptosis, CDC42, Retinal Pigment Epithelium, Pathogenesis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, lcsh:Ophthalmology, medicine, Humans, Interleukin 6, Cells, Cultured, Cytoskeleton, Retinal pigment epithelium, biology, business.industry, Interleukin-6, Age-related macular degeneration, Epithelial Cells, General Medicine, Anatomy, eye diseases, Actins, Cell biology, Biomechanical Phenomena, Vascular endothelial growth factor, Ophthalmology, 030104 developmental biology, medicine.anatomical_structure, Cytokine, chemistry, lcsh:RE1-994, 030221 ophthalmology & optometry, biology.protein, sense organs, Stress, Mechanical, business, Research Article
Abstract

Background The pathogenesis of age-related macular degeneration (AMD) is complex. It has been shown that vitreomacular traction (VMT) plays a role in the pathogenesis of AMD. We speculate that the continuous stretch induced by VMT might impair the function of retinal pigment epithelium (RPE) cells and it might also be involved in the progression of AMD. Methods Cultured ARPE-19 cells were subjected to cyclic stretch on the Flexcell Strain system at a level of 25% increment on the surface area for 8 h, 14 h, 20 h, 24 h. In another group, the stretch was withdrawn at 14 h and the cell cultured for another 6 h. Then, we observed the changes in morphology, apoptosis and expression of interleukin 6 (IL6) and vascular endothelial growth factor (VEGF) in RPE cells under stretch. Results We found that stretch induced the RPE cells to change from a spreading shape into a rounded shape, and that the morphological changes were positively correlated with the duration of the stretch. The expression of pFAK397 and pRac1/cdc42 were elevated in a time-dependent fashion. The stretch resulted in an increase in the apoptosis ratio, with Bcl2, Bax and p53 also showing time-dependent changes. In addition, up-regulation of IL6 and VEGF expression levels was also observed. After withdrawal of the stretch, all of these changes were significantly diminished. Conclusion Stretch may induce morphological, cell apoptosis, and up-regulation of cytokines changes in RPE cells, indicating that cyclic stretching may participate in the progression of AMD by impeding the functions of the RPE.

Published
2017

20. Proteomic analysis of aqueous humor from patients with primary open angle glaucoma

Author

Duan, Xiaoming, Xue, Peng, Wang, Ningli, Dong, Zhe, Lu, Qingjun, and Yang, Fuquan

Subjects
Adult, Male, Proteomics, Silver Staining, genetic structures, Blotting, Western, Middle Aged, eye diseases, Lipocalins, Mass Spectrometry, Aqueous Humor, Intramolecular Oxidoreductases, Case-Control Studies, Humans, Electrophoresis, Gel, Two-Dimensional, Female, sense organs, Eye Proteins, Glaucoma, Open-Angle, Research Article
Abstract

Purpose Primary open angle glaucoma (POAG) is a leading cause of irreversible blindness on a global level. Researchers have yet to specify the exact mechanisms of POAG; the respective relationships between POAG and elevated intraocular pressure (IOP), as well as optic neuropathy, remain particularly unclear. It is known, however, that the expression profile for some proteins in the aqueous humor (AH) changes in some diseases, and that AH changes play important roles in elevated IOP. To identify the possible roles of these AH proteins in POAG, a proteomic analysis of the AH compositions of POAG patients’ eyes was performed and compared with those derived from paired, non-POAG cataract (control) eyes. Methods We used Bradford’s method to determine total protein concentration in AH, and analyzed separation profiles via two-dimensional (2D) gel electrophoresis. We used silver stain to determine gel proteins, and analyzed separation profiles to assess spot density differences between POAG and non-POAG patients. These gel spots were isolated and identified via mass spectrometry. Prostaglandin H2 D-isomerase (PGDS) in AH were analyzed by western Blotting. Results There was no significant difference between the total protein concentration in AH of POAG patients and that in AH of non-POAG patients. A total of seven spots were increased in 2D gels from POAG patients. The spots were derived from PGDS, caspase 14 precursor, transthyretin, cystain C, albumin precursor, and tranferrin. And PGDS in AH from patients was more than from controls. Conclusions The protein composition in AH was significantly different in POAG patients versus non-POAG patients. The identified proteins could be a potential biomarker for POAG and may play a role in the mechanisms of elevated IOP and optic neuropathy in POAG.

Published
2010

21. Proteomic analysis of aqueous humor from patients with myopia

Author

Duan, Xiaoming, Lu, Qingjun, Xue, Peng, Zhang, Hongjie, Dong, Zhe, Yang, Fuquan, and Wang, Ningli

Subjects
Adult, Aged, 80 and over, Male, Proteomics, genetic structures, Vitamin D-Binding Protein, Molecular Sequence Data, Middle Aged, eye diseases, Mass Spectrometry, Aqueous Humor, Case-Control Studies, Myopia, Humans, Electrophoresis, Gel, Two-Dimensional, Female, sense organs, Amino Acid Sequence, Eye Proteins, Research Article, Aged
Abstract

Purpose The mechanism of axial elongation in the myopic eyeball remains to be elucidated. It is known that the expression profile for some proteins in the aqueous humor (AH) changes in some diseases. Accordingly, determinations of these AH proteins may serve to understand their potential role in this pathogenesis. To identify the possible mechanism in myopia development, a proteomic analysis of the AH composition from high myopic eyes (patients) was performed and compared with that of the AH composition from non-myopic cataract eyes (controls). Methods Total protein concentration in AH was determined by the Bradford method, and separation profiles were analyzed by two-dimensional (2D) gel electrophoresis. Protein in gel was determined by silver stain, and the separation profiles were analyzed to assess spot density changes between myopia and non-myopia patients. These spots in gel were isolated and identified by mass spectrometry. Results The total protein concentration in AH with high myopia was significantly greater than that of non-myopia. A total of six spots were significantly increased in 2D gels from high myopia. The spots were derived from albumin, transthyretin, and a vitamin D-binding protein. Conclusions The protein composition in AH was significantly different between myopia and non-myopia. The identified proteins could be a potential biomarker for high myopia development and may play a role in the mechanisms of myopia ocular axial elongation.

Published
2008

24. Glaucoma and the Role of Cerebrospinal Fluid Dynamics

Author

Zhang, Zheng, primary, Liu, Danli, additional, Jonas, Jost B., additional, Wu, Shen, additional, Kwong, Jacky M. K., additional, Zhang, Jingxue, additional, Liu, Qian, additional, Li, Lei, additional, Lu, Qingjun, additional, Yang, Diya, additional, Wang, Jinda, additional, and Wang, Ningli, additional

Published
2015
Full Text
View/download PDF

25. Cyclic stretch induced-retinal pigment epithelial cell apoptosis and cytokine changes.

Author

Shen Wu, Qingjun Lu, Ningli Wang, Jingxue Zhang, Qian Liu, Meng Gao, Jinqiu Chen, Wu Liu, Liang Xu, Wu, Shen, Lu, Qingjun, Wang, Ningli, Zhang, Jingxue, Liu, Qian, Gao, Meng, Chen, Jinqiu, Liu, Wu, and Xu, Liang

Abstract

Background: The pathogenesis of age-related macular degeneration (AMD) is complex. It has been shown that vitreomacular traction (VMT) plays a role in the pathogenesis of AMD. We speculate that the continuous stretch induced by VMT might impair the function of retinal pigment epithelium (RPE) cells and it might also be involved in the progression of AMD.Methods: Cultured ARPE-19 cells were subjected to cyclic stretch on the Flexcell Strain system at a level of 25% increment on the surface area for 8 h, 14 h, 20 h, 24 h. In another group, the stretch was withdrawn at 14 h and the cell cultured for another 6 h. Then, we observed the changes in morphology, apoptosis and expression of interleukin 6 (IL6) and vascular endothelial growth factor (VEGF) in RPE cells under stretch.Results: We found that stretch induced the RPE cells to change from a spreading shape into a rounded shape, and that the morphological changes were positively correlated with the duration of the stretch. The expression of pFAK397 and pRac1/cdc42 were elevated in a time-dependent fashion. The stretch resulted in an increase in the apoptosis ratio, with Bcl2, Bax and p53 also showing time-dependent changes. In addition, up-regulation of IL6 and VEGF expression levels was also observed. After withdrawal of the stretch, all of these changes were significantly diminished.Conclusion: Stretch may induce morphological, cell apoptosis, and up-regulation of cytokines changes in RPE cells, indicating that cyclic stretching may participate in the progression of AMD by impeding the functions of the RPE. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

30. Author Response: Optic Neuropathy Secondary to Spontaneous Intracranial Hypotension (SIH) as Related to Experimental Primate Model

Author

Yang, Diya, primary, Fu, Jidi, additional, Hou, Ruowu, additional, Liu, Kegao, additional, Jonas, Jost B., additional, Wang, Huaizhou, additional, Chen, Weiwei, additional, Li, Zhen, additional, Sang, Jinghong, additional, Zhang, Zheng, additional, Liu, Sumeng, additional, Cao, Yiwen, additional, Xie, Xiaobin, additional, Ren, Ruojin, additional, Lu, Qingjun, additional, Weinreb, Robert N., additional, and Wang, Ningli, additional

Published
2014
Full Text
View/download PDF

31. Chitosan Promoted the Corneal Epithelial Wound Healing via Activation of ERK Pathway.

Author

Cui, Rui, Lu, Qingjun, Teng, Yufei, Li, Kun, and Li, Na

Subjects
*CHITOSAN, *CORNEA, *WOUND healing, *EXTRACELLULAR signal-regulated kinases, *ANIMAL models in research
Abstract

Purpose: To investigate the mechanism of chitosan promoting corneal wound healing though evaluating its effect on extracellular signal-regulated kinases (ERK) and p38 pathway activity in a rabbit animal model. Methods: Cell proliferation and migration assay were performed 24 hours after chitosan treatment. The activity of ERK and p38 pathways was detected by using immunofluorescence and Western blotting in the presence of chitosan and an ERK inhibitor. In vivo study of epithelial debridement wounds was performed on 8 mm rabbit corneas in the presence of chitosan and an ERK pathway inhibitor. Results: Immunostaining with Ki67 and migrating assay showed that chitosan could upregulate the cell proliferation and promote the cell migration. Chitosan activated the ERK pathway in 5 min to 30 min after treatment but did not affect the p38 pathway. ERK inhibitor PD98059 can inhibit the chitosan-stimulated ERK phosphorylation. Chitosan increased the corneal epithelial wound closure in organ culture and ERK inhibition with PD98059 blocked the effect of chitosan on wound healing. Conclusions: Chitosan promoted corneal epithelial proliferation and migration during the wound healing in rabbits’ eye. Chitosan-stimulated epithelial wound healing is partially mediated through the activation of the ERK pathway but not the p38 pathway. [ABSTRACT FROM PUBLISHER]

Published
2017
Full Text
View/download PDF

32. Glaucoma Considered as an Imbalance Between Production and Clearance of Neurotoxins

Author

Yang, Diya, primary, Fu, Jidi, additional, Hou, Ruowu, additional, Liu, Kegao, additional, Jonas, Jost B., additional, Wang, Huaizhou, additional, Chen, Weiwei, additional, Li, Zhen, additional, Sang, Jinghong, additional, Zhang, Zheng, additional, Liu, Sumeng, additional, Cao, Yiwen, additional, Xie, Xiaobin, additional, Ren, Ruojin, additional, Lu, Qingjun, additional, Weinreb, Robert N., additional, and Wang, Ningli, additional

Published
2014
Full Text
View/download PDF

33. Optic Neuropathy Induced by Experimentally Reduced Cerebrospinal Fluid Pressure in Monkeys

Author

Yang, Diya, primary, Fu, Jidi, additional, Hou, Ruowu, additional, Liu, Kegao, additional, Jonas, Jost B., additional, Wang, Huaizhou, additional, Chen, Weiwei, additional, Li, Zhen, additional, Sang, Jinghong, additional, Zhang, Zheng, additional, Liu, Sumeng, additional, Cao, Yiwen, additional, Xie, Xiaobin, additional, Ren, Ruojin, additional, Lu, Qingjun, additional, Weinreb, Robert N., additional, and Wang, Ningli, additional

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results