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Abstract Background Both lipid metabolism and cyclic RNAs (circRNAs) have been found to be involved in pancreatic adenocarcinoma (PAAD) progression, but the relationship between lipid metabolism and circRNAs remains unclear. Methods The expression levels of miR-223-3p, circ_0124346, and acyl-CoA synthetase long chain family member 3 (ACSL3) were determined through qRT-PCR and Western blot analysis. Cell proliferation was evaluated using the CCK-8 and EdU incorporation assays. Cholesterol (CH) and triglyceride (TG) levels were quantified using relevant kits. The relationships between miR-223-3p and circ_0124346 or ACSL3 mRNA were examined by bioinformatics analysis, luciferase reporter, RNA-RNA pull-down, and RIP assays. Results We observed a significant elevation in circ_0124346 expression in both pancreatic adenocarcinoma (PAAD) tissues and cell lines, and its expression level was shown to be correlated with tumor size. Circ_0124346 stimulated cell proliferation and facilitated lipid synthesis in PAAD cells. Additionally, we found that circ_0124346 functioned as a sponge for miR-223-3p, preventing miR-223-3p’s binding to the 3'-UTR of ACSL3 mRNA, which subsequently led to an elevation in ACSL3 expression and promoted lipid synthesis. Accordingly, circ_0124346 knockdown resulted in a significant decrease in lipid synthesis and cell proliferation in PAAD cells, with partial reversal of these effects achieved via inhibiting miR-223-3p or overexpressing ACSL3. Conclusion Our study demonstrated that circ_0124346 regulates lipid metabolism in PAAD cells via the miR-223-3p/ACSL3 axis, suggesting that targeting circ_0124346 may serve as a potential strategy for treating PAAD and assisting in its diagnosis.

Aquatic ecosystems can harbour more than one non-native fish species and this can represent a threat due to trophic interactions with native fishes. However, research on interactions amongst multiple co-occurring native and non-native fish remains scarce. In this study, 551 organisms from 44 native fish, 11 non-native fish, 35 macroinvertebrates (of which one was non-native), together with 162 samples of basal resources were collected from six rivers of the Lower Pearl River Basin of China. Nitrogen and carbon stable isotope analysis was used to calculate community-wide trophic metrics and the degree of trophic overlap between native and non-native fish at both the community and functional feeding group level, together with diet composition. At the community level, there was a high degree of trophic niche overlap between native and non-native fish as a result of similarities in trophic characteristics. At the functional feeding group level, both native and non-native functional feeding groups demonstrated the capacity to occupy the niche space of each other. A significant trophic niche overlap, exceeding 50%, was found between non-native detritivorous and omnivorous fish, suggesting competition. The difference in diet composition between some native and non-native fish depended on the category of diet source across the rivers, suggesting dietary segregation. Albeit limited, the present findings suggest that trophic interaction between native and non-native fish is likely to reach a dynamic equilibrium status in the community owing to trophic segregation of fish species and the antagonistic effects amongst non-native fish.

Abstract Gut microbiota is related to host fitness, and influenced by geographical locations and habitats. Pomacea canaliculata is a malignant invasive alien snail that threatens agricultural production and ecosystem functions worldwide. Clarifying the general rules of the gut microbial community structure and function of the snails in different geographical locations and habitats is of great significance for understanding their invasion at different spatial scales. This study used high‐throughput sequencing technology to compare and analyze the differences in community structure and function of gut microbiota in P. canaliculata from five geographical locations (Liuzhou, Yulin, Nanning, Wuzhou, and Hezhou) and three different habitats (pond, paddy field, and ditch) in Guangxi Province. The results showed that intestinal microbial alpha diversity of P. canaliculata was higher in Liuzhou, Yulin, lower in Nanning, Wuzhou, Hezhou, and higher in ponds compared with paddy fields and ditches. The dominant phyla of gut microbiota in snails were Firmicutes, Cyanobacteria, Proteobacteria, Fusobacteriota, Bacteroidota, and the dominant genus was Lactococcus. The community structure of gut microbiota in snails varied significantly across different geographical locations and habitats, and the phyla Firmicutes, Cyanobacteria had significantly higher relative abundance in snails collected from Nanning and Yulin, respectively. Moreover, the relative abundance of gut functional microbiota associated with human disease in P. canaliculata was significantly affected by geographical locations and habitats, and with the highest abundance in ponds. However, the relative abundance of functional microbiota related to metabolism, genetic information processing, organizational system, environmental information processing, and cellular processes were only significantly affected by geographical locations. Collectively, geographical locations and habitats had significantly different effects on the community structure and function of gut microbiota in P. canaliculata, and the greater differences were caused by geographical locations rather than by habitats.

Abstract In the Anthropocene, non-native freshwater fish introductions and translocations have occurred extensively worldwide. However, their global distribution patterns and the factors influencing their establishment remain poorly understood. We analyze a comprehensive database of 14953 freshwater fish species across 3119 river basins and identify global hotspots for exotic and translocated non-native fishes. We show that both types of non-native fishes are more likely to occur when closely related to native fishes. This finding is consistent across measures of phylogenetic relatedness, biogeographical realms, and highly invaded countries, even after accounting for the influence of native diversity. This contradicts Darwin’s naturalization hypothesis, suggesting that the presence of close relatives more often signifies suitable habitats than intensified competition, predicting the establishment of non-native fish species. Our study provides a comprehensive assessment of global non-native freshwater fish patterns and their phylogenetic correlates, laying the groundwork for understanding and predicting future fish invasions in freshwater ecosystems.

[Objective] Pressure regulation systems, serving as one of the vital links between long-distance pipelines and urban gas pipeline networks, play a significant role in the successful implementation of the “Hydrogen Society” Program. However, the contrasting physical properties of hydrogen and natural gas impose challenges to the effectiveness of process control in pressure regulation. [Methods] This study employed a combined approach involving decompression and pressure regulation experiments conducted on pure hydrogen and hydrogen-enriched compressed natural gas(HCNG), along with dynamic simulations of pressure regulation. By establishing a foundation for assessing the pressure stabilization effect of the decompression and pressure regulation system, considering factors such as pressure stabilization accuracy, response time, and fitness function, a sensitivity analysis was performed to investigate the influence of various parameters, including hydrogen blending ratios, flow fluctuation cycles, downstream flow change ranges, pipeline transmission pressures, as well as proportional, integral, and derivative parameters(PID). [Results] The following results were obtained:(1) With more frequent disturbances, gas flow rates increased, leading to larger system fluctuation ranges. Consequently, achieving pressure stabilization became more challenging for the decompression and pressure regulation system. This finding highlighted the importance of restraining the movement of pure hydrogen and HCNG at high flow rates in transmission pipelines.(2) The flow rate of the pressure regulation system carried sinusoidal fluctuations. Setting the pressure stabilization accuracy at ±1.5% and the proportional and integral parameters within the range of 1 to 2, the decompression and pressure regulation experiments showed that the decompression and pressure regulation of pure hydrogen and HCNG could be substantially achieved within the required pressure range of urban gas pipelines.(3) Pure hydrogen exhibited greater instantaneous fluctuations compared to natural gas when flowing through pipelines at identical rates of transmission.As a consequence, the response time and adaptability function of the control system gradually increased with higher hydrogen blending ratios. Moreover, the instantaneous pressure fluctuation of pure hydrogen reached 1.15 times that of pure methane, resulting in a response time and adaptability function of the control system that increased to 1.13 and 2.68 times those of pure methane, respectively. Under the same proportional and integral parameters, it was more challenging to maintain the pressure stabilization for the hydrogen-containing gas. Hence, decreasing the integral parameter or increasing the proportional parameter proportionally to the hydrogen blending ratio is recommended, to attain the same or even better pressure stabilization effect as observed in natural gas scenarios. [Conclusion] The research findings provide theoretical insights to guide the pressure regulation practices of pipelines carrying pure hydrogen and HCNG.

Background:. With the popularity of liposuction surgery, more awareness should be obtained regarding complications. Liposuction has been thought of as a safe procedure with a very low incidence of major complications. However, life-threatening risks of liposuction have rarely been reported. Methods:. We present a case of a 36-year-old woman who developed cardiac arrest during a liposuction procedure, and we present a literature review. Results:. She was previously healthy and had no risk factors for pulmonary embolism. The diagnosis was made based on clinical presentation and the presence of an electrolyte disorder and a positive sign on computed tomography pulmonary angiogram (CTPA). Mild hypothermia treatment, symptomatic treatment, and supportive therapy were applied. As the respiratory and circulation were smooth, she was discharged to a rehabilitation hospital. Seven months after discharge, the patient was still in a coma with eye opening. Conclusions:. Spinal anesthesia, pulmonary embolism, and hyperkalemia are the most probable contributors to the cardiac arrest observed during the liposuction procedure in this specific case. There is a heightened imperative to vigilantly monitor for critical incidents during these operations and to meticulously identify associated risk factors during liposuction.

Background A better understanding of T cells in lung cancer and their distribution across tumor-adjacent lungs and peripheral blood is needed to improve efficacy and minimize toxicity from immunotherapy to lung cancer patients.Methods Here, we performed CDR3β TCR sequencing of 136 samples from 20 patients with early-stage NSCLC including peripheral blood mononuclear cells, tumors, tumor edges (

Abstract The aim of this study was to understand the dynamics of multidimensional poverty and its determinants among mid-aged and older adults. We used 2011, 2013, 2015, and 2018 data from the China Health and Retirement Longitudinal Study. We utilised basic descriptive statistics, the poverty transition matrix, Kaplan–Meier estimates, and the discrete-time proportional hazards model for data analysis. From 2011 to 2018, the incidence of multidimensional poverty among mid-aged and older people basically decreased, but the average poverty intensity remained stable. Most mid-aged and older adults had transient multidimensional poverty. The longer an individual remained multidimensionally poor, the smaller the probability of exit from poverty; The longer an individual remained nonpoor after escaping from poverty, the smaller the probability of returning to poverty. As to other factors, individual characteristics, family structure, living arrangements, social capital, and living areas significantly affected the risks of multidimensional poverty exit and reentry. Based on these results, the government should implement targeted interventions for frail older adults with the identified characteristics to prevent them from persistent multidimensional poverty or return to poverty.