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The purpose of this study was to explore the relationship between dynamic enhancement of magnetic resonance, miR-27, miR-155 expression and breast cancer. One hundred breast cancer patients were selected as the observation group, and another 60 patients with benign breast lesions were selected as the control group. This study compared the two groups7 DCE-MRI quantitative parameters [transport constant (Ktrans), rate constant (Kep), extravascular intercellular space fraction (Ve)] and the expression of miR-27 and miR-155, and then performed correlation analysis to evaluate its diagnostic power. The expression levels of Ktrans, Kep, miR-27 and miR-155 in the observation group were higher than those in the control group (P<0.05). The expression levels of Ktrans and Kep in breast cancer patients were significantly positively correlated with the expression levels of miR-27 and miR-155 (P<0.05). Ktrans, Kep, miR-27 and miR-155 were independent influencing factors of breast cancer (P<0.05). The expression of KtranS, Kep, miR-27, miR-155 is related to histological grade, clinical stage, lymph node metastasis, progesterone and estrogen receptor expression (P<0.05). The AUC of the combined diagnosis of breast cancer by DCE-MRI quantitative parameters, miR-27, miR-155 expression is 0.905. DCE-MRI quantitative parameters (Ktrans, Kep) and the expression levels of miR-27 and miR-155 were up-regulated in breast cancer, and were related to pathological features. [ABSTRACT FROM AUTHOR]

Celastrus orbiculatus Thunb. has been used as a remedy against cancer and inflammatory diseases for thousands of years in China. Maspin is expressed in normal cells and downregulated in prostate tumor cells. The underlying mechanisms between C. orbiculatus e xtract ( COE) a nd maspin remain unclear. In the present study, 3 target-specific 19-25 nucleotide maspin small interfering RNAs were designed and synthesized to knockdown maspin expression. The effects of COE on MGC-803/maspin- cell proliferation were evaluated by the MTT assay. Apoptosis was measured by flow cytometry. Invasive activity was measured with the Transwell assay and the associated molecular mechanisms were assessed by western blot analysis. The results demonstrated that COE significantly promoted the expression of maspin (P<0.01) to induce apoptosis and inhibit invasion and migration in MGC803 cells. The expression levels of phosphorylated (p)-p38 mitogen-activated protein kinase (MAPK), phospho-extracellular regulated protein kinase (Erk), B cell lymphoma-2-associated X protein and caspase-3 were increased in the MGC-803/maspin- cells in a dose-dependent manner. The Erk, B-cell lymphoma 2, p-Akt, Akt and p-mechanistic target of rapamycin (mTOR) protein in MGC-803/maspin- cells were reduced in a dose-dependent manner. This indicated that COE may inhibit invasion and migration through phosphoinositide 3-kinase/Akt/mTOR and MAPK signaling pathways in MGC-803/maspin- cells. In conclusion, COE has the ability to improve the expression of maspin to induce apoptosis and inhibit invasion and migration in human gastric adenocarcinoma cells. [ABSTRACT FROM AUTHOR]

Plasma-and chemical-grafted amidoxime/carbon nanotber hybrids (p-AO/ CNFs and c-AO/CNFs) were utilized to remove2-"U(VI) andL1Am(II1) from aqueous solutions, seawater, and groundwater. Characteristic results indicated more nitrogen-containing groups in p-AO/CNFs compared to c-AO/CNFs. The maximum adsorption capacities of p-AO/CNFs at pH 3.5 and T = 293 K (588.24 mg of mU(VI)/g and 40.79 mg ofw(Am(llI)/g from aqueous solutions, respectively) were sipctifcantly higher than those of c-AO/CNFs (263.18 and 22.77 mg/g for238U(VI) and24 Am(I11), respectively), which indicated that plasma-grafting was a highly effective, low-cost, and environmentally friendly method. Adsorption of 2wU(VI) on AO/CNF5 from aqueous solutions was significantly higher than that of 238U(VI) from seawater and groundwater; moreover, AO/CNF5 displayed the highest effective selectivity for 238U(VI) compared to the other radionuclides. Adsorption of U(VI) onto AO/CNFs created inner-sphere complexes (e.g., U-C shells) as shown by X-ray absorption fine structure analysis, which was supported by surface complexation modeling. Three inner-sphere complexes gave excellent fits to pH-edge and isothermal adsorption of 238U(VI) on the AO/CNFs. These observations are crucial for the utilization of plasma-grafted, AO-based composites in the preconcentration and immobilization of lanthanides and actinides in environmental remediation. [ABSTRACT FROM AUTHOR]