Search

Your search keyword '"Lu, Zenghui"' showing total 138 results
Start Over Author "Lu, Zenghui"
138 results on '"Lu, Zenghui"'

12. Corrigendum to “Network pharmacology-based mechanism prediction and pharmacological validation of Xiaoyan Lidan formula on attenuating alpha-naphthylisothiocyanate induced cholestatic hepatic injury in rats” [J. Ethnopharmacol. 270 (2021) 113816]

Author

Wang, Meiqi, primary, Liu, Fangle, additional, Yao, Yufeng, additional, Zhang, Qiuyu, additional, Lu, Zenghui, additional, Zhang, Runjing, additional, Liu, Changhui, additional, Lin, Chaozhan, additional, and Zhu, Chenchen, additional

Published
2023
Full Text
View/download PDF

21. TMPO-AS1, a Novel E2F1-Regulated lncRNA, Contributes to the Proliferation of Lung Adenocarcinoma Cells via Modulating miR-326/SOX12 Axis

Author

Wei,Lin, Liu,Yugang, Zhang,Huijun, Ma,Yuefeng, Lu,Zenghui, Gu,Zhenning, and Ding,Chao

Subjects
Cancer Management and Research
Abstract

Lin Wei,1 Yugang Liu,1 Huijun Zhang,1 Yuefeng Ma,2 Zenghui Lu,1 Zhenning Gu,1 Chao Ding1 1Department of Thoracic Surgery, Xi’an Chest Hospital, Xi’an 710100, People’s Republic of China; 2Department of Thoracic Surgery, The Second Affiliated Hospital of Xi’an Jiao Tong University, Xi’an 710004, People’s Republic of ChinaCorrespondence: Chao DingDepartment of Thoracic Surgery, Xi’an Chest Hospital, Xi’an, Shaanxi Province 710100, People’s Republic of ChinaTel/Fax +86-29-62500053Email abroad2014@sohu.comBackground: TMPO-AS1, an antisense lncRNA located at human chromosome 12p23.1, has been identified as an oncogene involved in cell proliferation in various cancers, including LUAD. In this study, we aimed to explore the novel molecular mechanism of TMPO-AS1 underlying LUAD growth.Materials and Methods: The transcription levels of TMPO-AS1, miR-326, and SOX12 in LUAD tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR). The cell proliferation ability was evaluatect 3d by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis analysis was assessed by flow cytometry. The target relationship among TMPO-AS1, miR-326, and SOX12 and promoter activity of TMPO-AS1 was measured using dual-luciferase reporter assay. The protein levels of SOX12 in LUAD cells were determined by Western blot. ChIP-qPCR assay was performed to validate the direct binding between E2F1 and TMPO-AS1 promoter.Results: TMPO-AS1 was up-regulated in LUAD tissues as well as cell lines. Boosted TMPO-AS1 expression was positively correlated with poor prognosis and pathological stage in LUAD. Down-regulation of TMPO-AS1 could restrain the proliferation of LUAD cells through arresting the cell cycle at G0/G1 phase and inducing apoptosis in vitro. Mechanically, we demonstrated that TMPO-AS1 could modulate the proliferation of LUAD cells through increasing SOX12 expression level via sponging miR-326 in accordance with bioinformatics analysis and experimental validation. Furthermore, we identified that TMPO-AS1 could be activated by E2F transcription factor 1 (E2F1) as a novel target gene.Conclusion: TMPO-AS1 can modulate LUAD cell proliferation through E2F1/miR-326/SOX12 pathway.Keywords: lung adenocarcinoma, TMPO-AS1, cell proliferation, miR-326, SOX12, E2F1

Published
2020

29. Transcriptomic analysis of the salivary gland of medicinal leech Hirudo nipponia

Author

Chen Shijiang, You Huajian, Yanqi Liu, Ping Shi, and Lu Zenghui

Subjects
0301 basic medicine, Molecular biology, lcsh:Medicine, UniGene, Traditional Chinese medicine, Hirudo medicinalis, Biochemistry, Salivary Glands, Transcriptome, Database and Informatics Methods, Sequencing techniques, 0302 clinical medicine, Medicine and Health Sciences, Medicine, Chinese Traditional, Annelids, lcsh:Science, Phylogeny, Multidisciplinary, Salivary gland, Gene Ontologies, Thrombin, Eukaryota, RNA sequencing, Genomics, Anti-Bacterial Agents, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Anatomy, Sequence Analysis, Transcriptome Analysis, Research Article, China, DNA, Complementary, Bioinformatics, Sequence Databases, Leech, Antineoplastic Agents, Computational biology, Biology, Research and Analysis Methods, 03 medical and health sciences, Exocrine Glands, Leeches, Genetics, medicine, Animals, Saliva, Gene Library, Sequence Assembly Tools, Gene Expression Profiling, lcsh:R, Hirudo nipponia, Organisms, Anticoagulants, Biology and Life Sciences, Computational Biology, Proteins, Genome Analysis, Invertebrates, Gene expression profiling, Biological Databases, Molecular biology techniques, 030104 developmental biology, lcsh:Q, Sequence Alignment, Digestive System
Abstract

Hirudo nipponia (known as Shui Zhi in Chinese) is a well-known Chinese medicine with numerous active ingredients in its body, especially in its saliva. This native Chinese blood-sucking leech has been used for therapeutic purposes since before 100 AD. Modern Chinese physicians use it for a wide range of diseases. Genomic data and molecular information about the pharmacologically active substances produced by this medicinal leech are presently unavailable despite this organism's medicinal importance. In this study, we performed transcriptome profiling of the salivary glands of medicinal leech H. nipponia using the Illumina platform. In total, 84,657,362 clean reads were assembled into 50,535 unigenes. The obtained unigenes were compared to public databases. Furthermore, a unigene sequence similarity search and comparisons with the whole transcriptome of medical leech were performed to identify potential proteins. Finally, more than 21 genes were predicted to be involved in anticoagulatory, antithrombotic, antibacterial, anti-inflammatory and antitumor processes, which might play important roles in the treatment of various diseases. This study is the first analysis of a sialotranscriptome in H. nipponia. The transcriptome profile will shed light on its genetic background and provide a useful tool to deepen our understanding of the medical value of H. nipponia.

Published
2018

50. Complete mitochondrial genome of Hepialus xiaojinensis (Lepidoptera: Hepialidae).

Author

Chen, Shijiang, Shi, Ping, Zhang, Deli, Lu, Zenghui, and Li, Longyun

Subjects
HEPIALIDAE, INSECT genomes, MITOCHONDRIAL RNA, INSECT phylogeny, TRANSFER RNA genetics
Abstract

AlthoughHepialus xiaojinensisis important as the host ofOphiocordyceps sinensis, it remains poorly known especially in genetic structure and phylogeny status. To get a better understanding of it, the complete mitochondrial genome sequence ofH. xiaojinensiswas sequenced for the first time. The genome was 15 397 bp in size and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and an AT-rich region, and the gene composition and the arrangement of which were consistent with other insects of Hepialidae. The base composition of the genome was A (41.44%), T (39.68%), C (11.52%), and G (7.36%), with an AT content of 81.13%. Moreover, we have performed phylogenetic analysis ofH. xiaojinensisand other 11 Lepidoptera species. [ABSTRACT FROM PUBLISHER]

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results