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Your search keyword '"Lucas, Carmen M."' showing total 18 results
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18 results on '"Lucas, Carmen M."'

2. A defined mechanistic correlate of protection against Plasmodium falciparum malaria in non-human primates

Author

Douglas, Alexander D., Baldeviano, G. Christian, Jin, Jing, Miura, Kazutoyo, Diouf, Ababacar, Zenonos, Zenon A., Ventocilla, Julio A., Silk, Sarah E., Marshall, Jennifer M., Alanine, Daniel G. W., Wang, Chuan, Edwards, Nick J., Leiva, Karina P., Gomez-Puerta, Luis A., Lucas, Carmen M., Wright, Gavin J., Long, Carole A., Royal, Joseph M., and Draper, Simon J.

Published
2019
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3. Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru

Author

Lucas Carmen M, Escalante Ananias A, Branch OraLee H, Chenet Stella M, and Bacon David J

Subjects
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic and could render a vaccine ineffective if their antigenic sites were not represented in the vaccine. In this study, characterization of genetic variability was performed in major B and T-cell epitopes within vaccine candidate antigens in isolates of P. falciparum from Peru. Methods DNA sequencing analysis was completed on 139 isolates of P. falciparum collected from endemic areas of the Amazon basin in Loreto, Peru from years 1998 to 2006. Genetic diversity was determined in immunological important regions in circumsporozoite protein (CSP), merozoite surface protein-1 (MSP-1), apical membrane antigen-1 (AMA-1), liver stage antigen-1 (LSA-1) and thrombospondin-related anonymous protein (TRAP). Alleles identified by DNA sequencing were aligned with the vaccine strain 3D7 and DNA polymorphism analysis and FST study-year pairwise comparisons were done using the DnaSP software. Multilocus analysis (MLA) was performed and average of expected heterozygosity was calculated for each loci and haplotype over time. Results Three different alleles for CSP, seven for MSP-1 Block 2, one for MSP-1 Block 17, three for AMA-1 and for LSA-1 each and one for TRAP were identified. There were 24 different haplotypes in 125 infections with complete locus typing for each gene. Conclusion Characterization of the genetic diversity in Plasmodium isolates from the Amazon Region of Peru showed that P. falciparum T and B cell epitopes in these antigens have polymorphisms more similar to India than to Africa. These findings are helpful in the formulation of a vaccine considering restricted repertoire populations.

Published
2008
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6. Outbreak of Cutaneous Leishmaniasis in Peruvian Military Personnel Undertaking Training Activities in the Amazon Basin, 2010

Author

Repositorio Academico UPC, Oré Marianela, Sáenz Eliana, Cabrera, Rufino, Sanchez, Juan F., De Los Santos, Maxy, Lucas, Carmen M., Núñez, Jorge, Edgel, Kimberly A., Sopan, Justino, Fernández, Jorge, Carnero, G, Baldeviano, Christian, Arrasco, Juan C., Graf, Paul C. F., and Lescano, Andres G.

Subjects
parasitic diseases
Abstract

Military personnel deployed to the Amazon Basin are at high risk for cutaneous leishmaniasis (CL). We responded to an outbreak among Peruvian Army personnel returning from short-term training in the Amazon, conducting active case detection, lesion sample collection, and risk factor assessment. The attack rate was 25% (76/303); the incubation period was 2–36 weeks (median = 8). Most cases had one lesion (66%), primarily ulcerative (49%), and in the legs (57%). Real-time polymerase chain reaction (PCR) identified Leishmania (Viannia) braziliensis (59/61 = 97%) and L. (V.) guyanensis (2/61 = 3%). Being male (risk ratio [RR] = 4.01; P = 0.034), not wearing long-sleeve clothes (RR = 1.71; P = 0.005), and sleeping in open rooms (RR = 1.80; P = 0.009) were associated with CL. Sodium stibogluconate therapy had a 41% cure rate, less than previously reported in Peru (70%; P < 0.001). After emphasizing pre-deployment education and other basic prevention measures, trainees in the following year had lower incidence (1/278 = 0.4%; P < 0.001). Basic prevention can reduce CL risk in deployed militaries.

Published
2015
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7. Population genomics studies identify signatures of global dispersal and drug resistance in Plasmodium vivax

Author

Hupalo, Daniel N, primary, Luo, Zunping, additional, Melnikov, Alexandre, additional, Sutton, Patrick L, additional, Rogov, Peter, additional, Escalante, Ananias, additional, Vallejo, Andrés F, additional, Herrera, Sócrates, additional, Arévalo-Herrera, Myriam, additional, Fan, Qi, additional, Wang, Ying, additional, Cui, Liwang, additional, Lucas, Carmen M, additional, Durand, Salomon, additional, Sanchez, Juan F, additional, Baldeviano, G Christian, additional, Lescano, Andres G, additional, Laman, Moses, additional, Barnadas, Celine, additional, Barry, Alyssa, additional, Mueller, Ivo, additional, Kazura, James W, additional, Eapen, Alex, additional, Kanagaraj, Deena, additional, Valecha, Neena, additional, Ferreira, Marcelo U, additional, Roobsoong, Wanlapa, additional, Nguitragool, Wang, additional, Sattabonkot, Jetsumon, additional, Gamboa, Dionicia, additional, Kosek, Margaret, additional, Vinetz, Joseph M, additional, González-Cerón, Lilia, additional, Birren, Bruce W, additional, Neafsey, Daniel E, additional, and Carlton, Jane M, additional

Published
2016
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8. Outbreak of Cutaneous Leishmaniasis in Peruvian Military Personnel Undertaking Training Activities in the Amazon Basin, 2010

Author

Oré, Marianela, primary, Sáenz, Eliana, additional, Cabrera, Rufino, additional, Sanchez, Juan F., additional, De Los Santos, Maxy B., additional, Lucas, Carmen M., additional, Núñez, Jorge H., additional, Edgel, Kimberly A., additional, Sopan, Justino, additional, Fernández, Jorge, additional, Carnero, Andres M., additional, Baldeviano, G. Christian, additional, Arrasco, Juan C., additional, Graf, Paul C. F., additional, and Lescano, Andres G., additional

Published
2015
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9. A PfRH5-Based Vaccine Is Efficacious against Heterologous Strain Blood-Stage Plasmodium falciparum Infection in Aotus Monkeys

Author

Douglas, Alexander D., primary, Baldeviano, G. Christian, additional, Lucas, Carmen M., additional, Lugo-Roman, Luis A., additional, Crosnier, Cécile, additional, Bartholdson, S. Josefin, additional, Diouf, Ababacar, additional, Miura, Kazutoyo, additional, Lambert, Lynn E., additional, Ventocilla, Julio A., additional, Leiva, Karina P., additional, Milne, Kathryn H., additional, Illingworth, Joseph J., additional, Spencer, Alexandra J., additional, Hjerrild, Kathryn A., additional, Alanine, Daniel G.W., additional, Turner, Alison V., additional, Moorhead, Jeromy T., additional, Edgel, Kimberly A., additional, Wu, Yimin, additional, Long, Carole A., additional, Wright, Gavin J., additional, Lescano, Andrés G., additional, and Draper, Simon J., additional

Published
2015
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10. A FRET-Based Real-Time PCR Assay to Identify the Main Causal Agents of New World Tegumentary Leishmaniasis

Author

Tsukayama, Pablo, primary, Núñez, Jorge H., additional, De Los Santos, Maxy, additional, Soberón, Valeria, additional, Lucas, Carmen M., additional, Matlashewski, Greg, additional, Llanos-Cuentas, Alejandro, additional, Ore, Marianela, additional, Baldeviano, G. Christian, additional, Edgel, Kimberly A., additional, Lescano, Andres G., additional, Graf, Paul C. F., additional, and Bacon, David J., additional

Published
2013
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11. Natural Leishmania Infection of Lutzomyia auraensis in Madre de Dios, Peru, Detected by a Fluorescence Resonance Energy Transfer–Based Real-Time Polymerase Chain Reaction

Author

Valdivia, Hugo O., primary, Graf, Paul C. F., additional, Lucas, Carmen M., additional, De Los Santos, Maxy B., additional, Mundal, Kirk D., additional, Lescano, Andrés G., additional, Baldeviano, G. Christian, additional, Vera, Hubert, additional, Edgel, Kimberly A., additional, Fernandez, Roberto, additional, and Zorrilla, Victor O., additional

Published
2012
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18. Anemia and antibodies to the 19-kDa fragment of MSP1 during Plasmodium falciparum infection in Aotus monkeys.

Author

Gozalo AS, Lucas CM, Qin J, Hall BT, and Magill AJ

Subjects
Anemia parasitology, Anemia pathology, Animals, Antibodies, Protozoan blood, Antimalarials therapeutic use, Aotidae, Disease Models, Animal, Erythrocytes parasitology, Malaria, Falciparum complications, Malaria, Falciparum pathology, Mefloquine therapeutic use, Merozoite Surface Protein 1 administration & dosage, Monkey Diseases immunology, Monkey Diseases pathology, Parasitemia drug therapy, Parasitemia immunology, Plasmodium falciparum growth & development, Anemia immunology, Antibodies, Protozoan immunology, Malaria, Falciparum immunology, Merozoite Surface Protein 1 immunology, Monkey Diseases parasitology, Plasmodium falciparum immunology
Abstract

To determine whether antibodies to the 19-kDa fragment of merozoite surface protein 1 (MSP1(19)) help to control blood-stage Plasmodium falciparum infection, we performed a rechallenge experiment of previously infected Aotus monkeys. Monkeys previously exposed to the FVO strain of P. falciparum that did or did not develop high antibody titers to MSP1(19) and malaria-naïve monkeys were challenged with erythrocytes infected with the same strain. Prepatent periods were prolonged in previously infected monkeys compared with malaria-naïve monkeys. Previously infected monkeys with preexisting anti-MSP1(19) antibodies showed low peak parasitemias that cleared spontaneously. Previously infected monkeys that had no or low levels of pre-existing anti-MSP1(19) antibodies also showed low peak parasitemias, but because of low hematocrits, all of these animals required treatment with mefloquine. All previously malaria-naïve animals were treated because of high parasitemias. The results of this study suggest that antibody to the 19-kDa carboxy-terminal fragment of MSP1 plays a role in preventing the development of anemia, an important complication often associated with malaria.

Published
2007

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