Search

Your search keyword '"Luciana R. C. Barros"' showing total 4 results
Start Over Author "Luciana R. C. Barros"
4 results on '"Luciana R. C. Barros"'

3. A comparative analysis of noise properties of stochastic binary models for a self-repressing and for an externally regulating gene

Author

Guilherme Giovanini, Alan U. Sabino, Luciana R. C. Barros, and Alexandre F. Ramos

Subjects
noise in gene regulatory models, stochastic gene regulation, binary gene model, fluctuations on gene expression, Biotechnology, TP248.13-248.65, Mathematics, QA1-939
Abstract

This manuscript presents a comparison of noise properties exhibited by two stochastic binary models for: (ⅰ) a self-repressing gene; (ⅱ) a repressed or activated externally regulating one. The stochastic models describe the dynamics of probability distributions governing two random variables, namely, protein numbers and the gene state as ON or OFF. In a previous work, we quantify noise in protein numbers by means of its Fano factor and write this quantity as a function of the covariance between the two random variables. Then we show that distributions governing the number of gene products can be super-Fano, Fano or sub-Fano if the covariance is, respectively, positive, null or negative. The latter condition is exclusive for the self-repressing gene and our analysis shows the conditions for which the Fano factor is a sufficient classifier of fluctuations in gene expression. In this work, we present the conditions for which the noise on the number of gene products generated from a self-repressing gene or an externally regulating one are quantitatively similar. That is important for inference of gene regulation from noise in gene expression quantitative data. Our results contribute to a classification of noise function in biological systems by theoretically demonstrating the mechanisms underpinning the higher precision in expression of a self-repressing gene in comparison with an externally regulated one.

Published
2020
Full Text
View/download PDF

4. Genetic switches designed for eukaryotic cells and controlled by serine integrases

Author

Cintia Elisabeth Gomez Limia, Gustavo P. C. José, Elíbio L. Rech, E. O. Melo, Cintia M. Coelho, Cristiano Lacorte, L. M. G. Barros, Thais Torquato Sales, Stevens K. Rehen, Lilian Hasegawa Florentino, Mayna Da Silveira Gomide, Luciana Rodrigues Carvalho Barros, Maria L. Robledo, André M. Murad, Rayane Nunes Lima, Martín Hernán Bonamino, Marco Antônio de Oliveira, Mariana S. M. Almeida, MAYNA S. GOMIDE, UNB, THAIS T. SALES, UNB, LUCIANA R. C. BARROS, INCA, CINTIA G. LIMIA, INCA, MARCO A. DE OLIVEIRA, UNB, LILIAN HASEGAWA FLORENTINO, Cenargen, LEILA MARIA GOMES BARROS, Cenargen, MARIA L. ROBLEDO, INCA, GUSTAVO P. C. JOSÉ, MARIANA S. M. ALMEIDA, RAYANE N. LIMA, STEVENS K. REHEN, IDOR, CRISTIANO CASTRO LACORTE, Cenargen, EDUARDO DE OLIVEIRA MELO, Cenargen, ANDRE MELRO MURAD, Cenargen, MARTÍN H. BONAMINO, INCA, CINTIA M. COELHO, UNB, and ELIBIO LEOPOLDO RECH FILHO, Cenargen.

Subjects
Cell type, Expression systems, Cell, Arabidopsis, Medicine (miscellaneous), General Biochemistry, Genetics and Molecular Biology, Article, Serine, 03 medical and health sciences, Synthetic biology, Eukaryotic cells, 0302 clinical medicine, Plasmid, medicine, Animals, Humans, Promoter Regions, Genetic, lcsh:QH301-705.5, 030304 developmental biology, Recombination, Genetic, 0303 health sciences, biology, Integrases, Protoplasts, Fibroblasts, Embryonic stem cell, Integrase, Cell biology, medicine.anatomical_structure, Genetic circuit engineering, lcsh:Biology (General), biology.protein, Leukocytes, Mononuclear, Genetic switches, Cattle, General Agricultural and Biological Sciences, 030217 neurology & neurosurgery, Plasmids
Abstract

Recently, new serine integrases have been identified, increasing the possibility of scaling up genomic modulation tools. Here, we describe the use of unidirectional genetic switches to evaluate the functionality of six serine integrases in different eukaryotic systems: the HEK 293T cell lineage, bovine fibroblasts and plant protoplasts. Moreover, integrase activity was also tested in human cell types of therapeutic interest: peripheral blood mononuclear cells (PBMCs), neural stem cells (NSCs) and undifferentiated embryonic stem (ES) cells. The switches were composed of plasmids designed to flip two different genetic parts driven by serine integrases. Cell-based assays were evaluated by measurement of EGFP fluorescence and by molecular analysis of attL/attR sites formation after integrase functionality. Our results demonstrate that all the integrases were capable of inverting the targeted DNA sequences, exhibiting distinct performances based on the cell type or the switchable genetic sequence. These results should support the development of tunable genetic circuits to regulate eukaryotic gene expression., Gomide et al. generate unidirectional genetic switches to test the site-specific recombination function of six recently identified serine integrases in a range of eukaryotic cells. They report that one of the integrases Int13 is as active in vertebrate cells as the currently top performing integrases, Bxb1 and PhiC31.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results