110 results on '"Lulu Zhu"'
Search Results
2. Optimizing TSES method based on the environmental factors to select negative samples and its application in landslide susceptibility evaluation
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Yulong CUI, Lulu ZHU, Min XU, and Haibo MIAO
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landslide susceptibility evaluation ,relief algorithm ,negative sample ,tses method based on the optimixation of environmental factor ,random forest ,ancient landslide ,Geology ,QE1-996.5 ,Engineering geology. Rock mechanics. Soil mechanics. Underground construction ,TA703-712 - Abstract
Objective Landslide susceptibility evaluation is an important means for landslide disaster prevention and control. Unreasonable negative landslide samples will affect landslide susceptibility evaluation, thereby affecting landslide disaster prevention and control. Therefore, it is particularly critical to provide a reasonable negative sample selection method. Methods In view of the selection of negative landslide samples, the ancient landslides in Milin City, Xizang are taken as an example, and 10 environmental factors, including elevation, slope, slope aspect, slope position, distance to road, distance to fault, distance to water system, topographic relief, lithology and land use type, are selected. The Relief algorithm is used to calculate the contribution values of environmental factors and to select the optimized environmental factors. The target space exteriorization sampling (TSES) method based on the optimization of environmental factors is applied to select negative samples as the input variables of the random forest (RF)model with excellent performance.Then, the optimized environmental factors and positive/negative samples are combined to predict the landslide susceptibility of Milin city, and the confusion matrix and receiver operating characteristic (ROC) curve are used to evaluate the prediction performance.To test the effectiveness and advancement of the TSES method optimized by environmental factors, the coupled information method and TSES method are respectively used to select negative landslide samples and constructs RF models to conduct comparative research with the RF model constructed by the TSES method optimized by environmental factors. Results The results show that the evaluation effect of the RF model constructed by the optimized TSES method based on environmental factors is better with an ACC value of 93.7% and an AUC value of 0.987, both of which are greater than those of the RF models constructed by the coupling information method and the TSES method. Conclusion The TSES optimized by environmental factors can improve the accuracy of the RF model, solve the problems of environmental factor selection in multifactor constrained sampling, and provide a new approach to collect negative landslide samples for landslide susceptibility evaluation.
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- 2024
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3. Holothuria leucospilota polysaccharides (HLP) ameliorate colitis rats via regulation of the metabolic profiling and TLR4/NLRP3 signaling pathways
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Xin Zhang, Qiuge Shi, Maojie Hu, Kexue Zhu, Lulu Zhu, Jun Cao, and Chuan Li
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Holothuria leucospilota polysaccharides ,metabolomics ,short‐chain fatty acids ,ulcerative colitis ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Abstract Recently, the development of natural polysaccharides for ameliorating immunity and gut metabolism has attracted extensive attention. This study used Holothuria leucospilota polysaccharides (HLP) to explore the improvement mechanism in ulcerative colitis rats on perspectives of immunity and metabolism. The results showed that HLP increased goblet cells’ number and the content of tight junction proteins (zona occludens 1 and occludin) and improved intestinal barrier permeability. The levels of immune cytokines (IL‐4, IL‐6, IL‐10, IL‐18, TNF‐α, and IL‐1β) and the activity of oxidative stress‐related enzymes (superoxide dismutase, catalase, malondialdehyde, and glutathione peroxidase) were regulated. HLP regulated the related genes and proteins expression of immune cytokines, MAPK, and NLRP3 inflammasome. Furthermore, HLP treatment increased the concentration of short‐chain fatty acids (SCFAs) and regulated serum metabolic disorders by regulating amino acid metabolism, SCFA metabolism, and energy metabolism. These results provide a new perspective for developing HLP as a promising functional food for preventing and mitigating colitis.
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- 2024
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4. Landslide susceptibility zoning with five data models and performance comparison in Liangshan Prefecture, China
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Wei Xu, Yulong Cui, JiaZhu Wang, LingFeng Gong, and Lulu Zhu
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landslide susceptibility zoning ,utilizing evidence weight ,logistic regression ,geological hazards ,liangshan prefecture ,Science - Abstract
Liangshan Prefecture, located at the northeastern edge of the Hengduan Mountain System and within the southern section of the Sichuan-Yunnan tectonic belt in Sichuan Province, China, a region prone to landslides, collapses and debris flows due to its active tectonics, complex topography and significant river erosion. By analysing a dataset of environment factors and geological hazard catalogue, the research uses the Relief algorithm to identify critical influencing factors for each hazard type, selecting 10, 9 and 9 factors for landslides, collapses and debris flows, respectively. Five models are used to assess the vulnerability of these hazards: the Information Value model, the Evidence Weight model, the Logistic Regression model, and both the Evidence Weight-Logistic Regression and the Information Value-Logistic Regression coupled models. The effectiveness of these models is confirmed by confusion matrix and ROC curve analyses, with the combined models showing particularly high accuracy in assessing susceptibility. High risk zones were identified in specific areas and along major fault zones in Liangshan Prefecture. The research provides significant insights into the susceptibility of geological hazards in mountainous and canyon regions, offering a comprehensive approach that goes beyond the limitations of single model applications. This methodology not only provides more accurate and comprehensive results, but also serves as a fundamental reference for geological hazard mitigation and management in Liangshan Prefecture, potentially benefiting similar regions worldwide.
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- 2024
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5. Effects of replacing wheat bran with palm kernel cake or fermented palm kernel cake on the growth performance, intestinal microbiota and intestinal health of tilapia (GIFT, Oreochromis niloticus)
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Huajing Huang, Xiangqin Lin, Xiaoxue Meng, Yu Liu, Jiongting Fan, Lulu Zhu, Jiaxian Chen, Lu Zhang, Haifeng Mi, and Junming Deng
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palm kernel cake ,fermented palm kernel cake ,growth performance ,intestinal microbiota ,intestinal health ,Nutrition. Foods and food supply ,TX341-641 - Abstract
A nine-week feeding trial was conducted to evaluate the effects of replacing wheat bran (WB) with palm kernel cake (PKC) or fermented palm kernel cake (FPKC) on the growth performance, intestinal microbiota and intestinal health of genetically improved farmed tilapia (GIFT, Oreochromis niloticus) (initial weight 7.00 ± 0.01 g). Eleven isonitrogenous and isolipidic experimental diets were formulated by replacing 0, 20, 40, 60, 80, and 100% of dietary WB with PKC or FPKC. Replacement of WB with PKC concentrations up to 80% had no significant effect on the growth rate of tilapia or feed utilisation (p > 0.05). FPKC improved the growth performance of tilapia, with optimum growth achieved at 40% replacement level (p
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- 2024
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6. Case report: Successful treatment of advanced colon cancer in an eighty-year-old man with long-term and multi-stage endoscopic minimally invasive therapy
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Nana Zhang, Lulu Zhu, Yan Liu, Xiaolong Chen, Bifang Zhang, Chunhong Wen, Huayu Zhang, Qinglin Tang, and Mingqing Zhang
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colonic neoplasms ,aged ,endoscopy ,argon plasma coagulation ,surgery ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundNo previous studies have reported on the use of minimally invasive endoscopic therapy for colon cancer in older patients.Case presentationAn 80-year-old man was admitted to our hospital with haematochezia and diagnosed with advanced colon cancer in 2018. Traditional surgical care was rejected by his family. We successfully treated the patient with multiple minimally invasive endoscopic therapies, such as argon plasma coagulation, from 2018 to 2021.ConclusionInvasive endoscopic therapy is a feasible way to treat colon cancer in older patients.
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- 2024
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7. Serum metabonomic study to explore the amelioration mechanisms of co‐fermented collagen peptide‐jackfruit juice on the immunosuppressed mice
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Tingting Ma, Ming Cheng, Xin Wen, Kexue Zhu, Xin Zhang, Chao Zhao, Hesham R. El‐Seedi, Xiangdong Lin, Yongcheng Li, Lulu Zhu, Jun Cao, and Chuan Li
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collagen ,immunosuppressed mice ,metabolomics ,oxidative stress ,pathway analysis ,Food processing and manufacture ,TP368-456 ,Toxicology. Poisons ,RA1190-1270 - Abstract
Abstract The main purpose of this study was to investigate the improvement effect and serum metabolomic analysis of co‐fermented collagen peptide‐jackfruit juice (FPJ) against immunosuppression in cyclophosphamide‐induced mice. Serum samples were analyzed using ultra‐performance liquid chromatography coupled with mass spectrometry (UPLC‐MS/MS) targeted metabolomics technology, including the normal group, model group, and FPJ group, and determine if such treatments were able to revert immunosuppression‐related metabolic changes to normal status. The results showed that 32 differential metabolites were upregulated and 42 metabolites were downregulated in the model group compared with the normal group. After FPJ intervention, there was a trend of normal callback. Adenosine, inosine, guanosine, 2‐deoxyadenosine, hypoxanthine, xanthine, d‐xylulose‐5‐phosphate, ribulose‐5‐phosphate, phenylpyruvic acid, hippuric acid, 3‐(3‐hydroxyphenyl)‐propionic acid, 3‐ureidopropionate, uridine, dCDP and malonic acid may be used as predictive markers of immunosuppression. Notably, these biomarkers were involved in purine metabolism, pentose phosphate pathway, phenylalanine metabolism, and pyrimidine metabolism. In this study, FPJ inhibited oxidative stress and improved the gut microbes by regulating the disorders of metabolic pathways. The metabolomics approach developed herein provides novel insight into the metabolic disturbances associated with immunosuppression, which will contribute to understanding the mechanism of FPJ on immunosuppression further and providing a basis for further research.
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- 2024
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8. Associations of dietary, sociodemographic, and anthropometric factors with anemia among the Zhuang ethnic adults: a cross-sectional study in Guangxi Zhuang Autonomous Region, China
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Zheng Wen, Jianxiong Long, Lulu Zhu, Shun Liu, Xiaoyun Zeng, Dongping Huang, Xiaoqiang Qiu, and Li Su
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Anemia ,Hemoglobin ,Diet ,Guangxi ,China ,Aging ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background After decades of rapid economic development, anemia remains a significant public health challenge globally. This study aimed to estimate the associations of sociodemographic, dietary, and body composition factors with anemia among the Zhuang in Guangxi Zhuang Autonomous Region, China. Methods Our study population from the baseline survey of the Guangxi ethnic minority Cohort Study of Chronic Diseases consisted of 13,465 adults (6,779 women and 6,686 men) aged 24–82 years. A validated interviewer-administered laptop-based questionnaire system was used to collect information on participants’ sociodemographic, lifestyle, and dietary factors. Each participant underwent a physical examination, and hematological indices were measured. Least absolute shrinkage and selection operator (LASSO) regression was used to select the variables, and logistic regression was applied to estimate the associations of independent risk factors with anemia. Results The overall prevalences of anemia in men and women were 9.63% (95% CI: 8.94–10.36%) and 18.33% (95% CI: 17.42─19.28%), respectively. LASSO and logistic regression analyses showed that age was positively associated with anemia for both women and men. For diet in women, red meat consumption for 5–7 days/week (OR = 0.79, 95% CI: 0.65–0.98, p = 0.0290) and corn/sweet potato consumption for 5–7 days/week (OR = 0.73, 95% CI: 0.55–0.96, p = 0.0281) were negatively associated with anemia. For men, fruit consumption for 5–7 days/week (OR = 0.75, 95% CI: 0.60–0.94, p = 0.0130) and corn/sweet potato consumption for 5–7 days/week (OR = 0.66, 95% CI: 0.46–0.91, p = 0.0136) were negatively correlated with anemia. Compared with a normal body water percentage (55–65%), a body water percentage below normal ( 65%) was positively correlated with anemia in men (OR = 1.73, 95% CI: 1.38–2.17, p
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- 2023
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9. Effects of Different Astaxanthin Sources on Fillet Coloration and Energy Deposition in Rainbow Trout (Oncorhynchus mykiss)
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Xiaoxue Meng, Fumei Yang, Lulu Zhu, Lingli Zhan, Toru Numasawa, and Junming Deng
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Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
A 9-week feeding trial was conducted to investigate the effects of different dietary sources of astaxanthin on fillet coloration, texture, and nutrient composition in rainbow trout (Oncorhynchus mykiss). Eight diets were formulated to contain 0, 25, 50, 75, 100, and 125 mg/kg astaxanthin from wall-broken Haematococcus pluvialis (WBHPA), 100 mg/kg astaxanthin from wall-unbroken H. pluvialis (WUHPA), or chemically synthesized astaxanthin (CSA). Each diet was fed to triplicate groups of rainbow trout (mean initial weight of 561 g) twice daily (07:00 and 17:00) to apparent satiation for 9 weeks. Results showed that at the 100 mg/kg astaxanthin inclusion level, the CAS group had higher fillet gross energy, dorsal fillet redness, and dorsal fillet color card score compared to the WBHPA-100 group, with both being higher than the WUHPA group (P0.05), but were higher than the WUHPA group. When WBHPA was used, the inclusion of 50–100 mg/kg decreased fillet lightness but increased fillet redness, while better fillet texture was served at 75–125 mg/kg. Dietary 25–125 mg/kg WBHPA inclusion increased fillet astaxanthin and gross energy concentrations, with minor effects on fatty acid compositions of fillet. Inclusion of over 100 mg/kg astaxanthin regardless of source decreased fillet threonine and serine contents, and the WBHPA-100 group had lower fillet glycine and alanine contents compared to the control group (P
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- 2024
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10. Effects of dietary peptidoglycan on Litopenaeus vannamei: Growth performance, disease resistance, non-specific immunity and transcriptome analysis of immune response
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Cuihong Hou, Lulu Zhu, Yudong Zheng, Lili Shi, Beiping Tan, and Shuang Zhang
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Peptidoglycan ,Litopenaeus vannamei ,Immunity ,Transcriptome analysis ,Aquaculture. Fisheries. Angling ,SH1-691 - Abstract
At present, peptidoglycan (PGN) is commonly used as an immunostimulant in shrimp. However, little has been known about its mechanism. In this study, comparative transcriptome analysis was employed to investigate the impact of dietary PGN on the immune response in Litopenaeus vannamei. A total of 720 healthy juvenile L. vannamei (initial weight 0.31 ± 0.05 g) were randomly evenly allocated to 18 fibreglass buckets as 6 groups, fed with six isonitrogen (41.05%) and isolipid diets (8.16%) containing PGN at the doses of 0 (control), 0.2, 1, 5, 25, and 125 mg/kg, respectively. After 8 weeks feeding trail, the growth performance, immunity, and transcriptome response of L. vannamei were analyzed. The results showed that the growth performance of L. vannamei was not significantly influenced by dietary PGN (P > 0.05). According to the analysis of weight gain rate (WGR), the optimum dietary PGN level was 71.46 mg/kg. Compared with the control group, the survival rate (SR) after Vibrio parahaemolyticus infection of L. vannamei were significantly increased by dietary PGN at 5 mg/kg and the SR after white spot syndrome virus (WSSV) infection was improved by dietary PGN at 25 mg/kg (P
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- 2023
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11. Optimizing Anesthetic Practices for Mud Crab: A Comparative Study of Clove Oil, MS-222, Ethanol, and Magnesium Chloride
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Lulu Zhu, Shanshan Qi, Ce Shi, Shujian Chen, Yangfang Ye, Chunlin Wang, Changkao Mu, Ronghua Li, Qingyang Wu, Xiaopeng Wang, and Yueyue Zhou
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Scylla paramamosain ,anesthetic ,physiological response ,antioxidant capacity ,neurotransmitters ,metabolomics ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Anesthesia serves as an effective method to mitigate the stress response in aquatic animals during aquaculture and product transportation. In this study, we assessed the anesthetic efficacy of clove oil, tricaine methane-sulfonate (MS-222), ethanol, and magnesium chloride by anesthesia duration, recovery time, 24-hour survival rate, and the behavior of mud crabs (Scylla paramamosain). Additionally, the optimal anesthetic concentration for varying body weights of mud crabs was also investigated. The results revealed that clove oil emerged as the optimal anesthetic for mud crabs, with a 24-hour survival rate surpassing those observed in MS-222 and magnesium chloride treatments. Ethanol caused amputation and hyperactivity in mud crabs. Regression analyses between the optimal anesthetic concentration of clove oil and the weight categories of 0.03–27.50 g and 27.50–399.73 g for mud crabs yielded the following equations: y = 0.0036 x3 − 0.1629 x2 + 1.7314 x + 4.085 (R2 = 0.7115) and y = 0.0437 x + 2.9461 (R2 = 0.9549). Clove oil exhibited no significant impact on serum cortisol, glucose, lactate content, aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities, or superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels in mud crabs across different treatment groups. Anesthesia induced by clove oil in mud crabs resulted in an increase in inhibitory neurotransmitters such as glycine. However, the recovery from anesthesia was associated with elevated levels of the excitatory neurotransmitters L-aspartic acid and glutamate. In conclusion, clove oil proves to be a safe and optimal anesthetic agent for mud crabs, exerting no physiological stress on the species.
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- 2023
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12. The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells
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Jie Liu, Zhenxing Ren, Lin Yang, Lulu Zhu, Yi li, Caiqun Bie, Helu Liu, Yichun Ji, Dongfeng Chen, Meiling Zhu, and Weihong Kuang
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Ferroptosis is a type of cell death induced by the iron-dependent accumulation of lipid hydroperoxides and reactive oxygen species (ROS) in cells. Inhibiting ferroptosis is important for improving the survival of transplanted bone marrow-derived mesenchymal stem cells (BMSCs). Although it is known that NOP2/Sun RNA methyltransferase 5 (NSUN5) post-transcriptionally regulates ferroptosis in BMSCs through RNA methylation, the precise mechanisms underlying these effects have not been reported. In this study, we demonstrate that NSUN5 is downregulated in erastin-induced ferroptosis in BMSCs. Ferroptosis was inhibited by the overexpression of NSUN5 or ferritin heavy chain/light-chain (FTH1/FTL) and was enhanced by NSUN5 knockdown. RNA immunoprecipitation experiments revealed that NSUN5 binds to FTH1/FTL, while NSUN5 depletion reduced the levels of 5-methylcytosine in FTH1/FTL RNA and increased intracellular iron concentrations, resulting in the downregulation of glutathione peroxidase 4 (GPX4) and the accumulation of ROS and lipid peroxidation products. Co-immunoprecipitation experiments demonstrated that the recognition of FTH1 and FTL by NSUN5 is dependent on the recruitment of tumor necrosis factor receptor-associated protein 1 (TRAP1). These results suggested that the NSUN5-FTH1/FTL pathway mediates ferroptosis in BMSCs and that the therapeutic targeting of components of this pathway may promote resistance to ferroptosis and improve the survival of transplanted BMSCs.
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- 2022
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13. High performance and resource efficient FFT processor based on CORDIC algorithm
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Yupu Zhao, Hong Lv, Jun Li, and Lulu Zhu
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Coordinate rotation digital computer (CORDIC) ,Multipath delayed commuting (MDC) ,Pipelined ,Fast Fourier transform (FFT) ,Telecommunication ,TK5101-6720 ,Electronics ,TK7800-8360 - Abstract
Abstract Fast Fourier Transform is widely used in communication and signal processing. I propose an improved multipath delay commutator pipelining architecture based on the radix-2 time decimation algorithm. By optimizing the intermediate data processing process and the first stage of pipelining, the architecture improves the system's computing speed and reduces the use of registers. I propose a multiplication scheme based on CORDIC and binary decomposition coding to realize complex number multiplication and constant multiplication and to eliminate the use of a multiplier. Experimental results suggest that proposed implementation has less latency and hardware utilization as compared to recently proposed implementations.
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- 2022
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14. Distinguishing feature of gut microbiota in Tibetan highland coronary artery disease patients and its link with diet
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Yulan Ma, Lulu Zhu, Zhijun Ma, Zhongshan Gao, Yumiao Wei, Youlu Shen, Lin Li, Xingli Liu, and Ming Ren
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Medicine ,Science - Abstract
Abstract The prevalence of coronary artery disease (CAD) in Tibetan Highlanders is lower than that in plain-living individuals, but the mechanism still unclear. Gut microbiota (GM) disorder is considered one of the potential factors involved in the pathogenesis of CAD, but the GM characteristics of Tibetan Highlanders suffering from CAD are unknown. We sequenced the V3-V4 region of the 16S ribosomal RNA of gut bacteria from fecal samples from Tibetan and Han CAD patients and healthy individuals inhabiting the Qinghai-Tibet Plateau, as well as from Han CAD patients and healthy individuals living at sea level, and we analyzed the GM characteristics of these subjects by bioinformatics analysis. The results showed that Tibetan Highlanders suffering from CAD had higher GM α-diversity, with differently distributed cluster compared with healthy Tibetan Highlanders and Han CAD patients living at high and low altitudes. Genera Catenibacterium, Clostridium_sensu_stricto, Holdemanella, and Ruminococcus 2 were enriched in Tibetan Highlanders suffering from CAD compared with healthy Tibetan Highlanders and Han CAD patients living at high- and low-altitudes. Prevotella was enriched in Tibetan Highlanders suffering from CAD compared with Han CAD patients living at high- and low-altitudes. Moreover, Catenibacterium was positively correlated with Prevotella. Additionally, Catenibacterium, Holdemanella, and Prevotella were positively correlated with fermented dairy product, carbohydrate and fiber intake by the subjects, while Clostridium_sensu_stricto was negatively correlated with protein intake by the subjects. In conclusion, our study indicated that Tibetan Highlanders suffering from CAD showed distinct GM, which was linked to their unique dietary characteristics and might associated with CAD.
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- 2021
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15. Gastroduodenal intussusception caused by gastric gastrointestinal stromal tumor in adults: a case report and literature review
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Wenbing Zhang, Haifeng Chen, Lulu Zhu, Zhiyuan Kong, Tingting Wang, and Weiping Li
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Medicine (General) ,R5-920 - Abstract
Intussusception mostly occurs in childhood and is rare in adults. Although intussusception can occur in any part of the gastrointestinal tract, gastroduodenal intussusception caused by a gastric tumor is relatively uncommon in clinical practice. A PubMed search identified 24 published cases of gastroduodenal intussusception caused by gastric gastrointestinal stromal tumor (GIST); however, it is possible that we missed other cases not included in PubMed. Here we report a case of gastroduodenal intussusception caused by gastric GIST in an 85-year-old man. He came to the hospital because of recurrent black stools. Plain computed tomography (CT) scan indicated a mass in the gastric antrum, with slight enhancement in the arterial phase on enhanced CT scan. He was diagnosed with GIST. In addition, images indicated that the mass overlapped into the duodenum, and gastroduodenal intussusception was thus considered. Gastroscopy showed a huge mass in the gastric body. According to the gastroscopy and CT results, gastroduodenal intussusception caused by a gastric tumor was considered. The patient underwent complete surgical removal, which revealed a mass originating from the gastric antrum and overlapping into the duodenum. The postoperative pathological diagnosis was intermediate-risk gastric GIST. The patient was followed up for 4 months without tumor recurrence.
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- 2022
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16. Dietary Histamine Impairs the Digestive Physiology Function and Muscle Quality of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)
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Yumeng Zhang, Hang Zhou, Yu Liu, Lulu Zhu, Jiongting Fan, Huajing Huang, Wen Jiang, Junming Deng, and Beiping Tan
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histamine ,hybrid grouper ,growth performance ,antioxidant response ,digestive physiology function ,muscle texture ,Therapeutics. Pharmacology ,RM1-950 - Abstract
An 8-week feeding experiment was conducted to investigate the effect of dietary histamine on growth performance, digestive physiology function and muscle quality in a hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). Seven isoproteic (50%) and isolipidic (11%) diets were prepared with various histamine inclusion levels of 0, 30, 60, 120, 240, 480 and 960 mg/kg in diets (actual contents were 72.33, 99.56, 138.60, 225.35, 404.12, 662.12 and 1245.38 mg/kg), respectively. Each diet was randomly assigned to triplicates of 30 juveniles (average body weight 14.78 g) per tank in a flow-through mariculture system. The increase in the dietary histamine level up to 1245.38 mg/kg made no significant difference on the growth rate and feed utilization of the grouper. However, the increased histamine content linearly decreased the activities of digestive enzymes, while no differences were observed in groups with low levels of histamine (≤404.12 mg/kg). Similarly, high levels of histamine (≥404.12 mg/kg) significantly damaged the gastric and intestinal mucosa, disrupted the intestinal tight junction structure, and raised the serum diamine oxidase activity and endotoxin level. Meanwhile, high doses of histamine (≥662.12 mg/kg) significantly reduced the activities of antioxidant enzymes, upregulated the relative expression of Kelch-like ECH-associated protein 1, and hardened and yellowed the dorsal muscle of grouper. These results showed that dietary histamine was detrimental to the digestive physiology function and muscle quality of the grouper, although it did compromise its growth performance.
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- 2023
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17. Characteristics of Ancient Shipwreck Wood from Huaguang Jiao No. 1 after Desalination
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Xinyou Liu, Lulu Zhu, Xinwei Tu, Changjun Zhang, Houyi Huang, and Anca Maria Varodi
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microscopy ,waterlogged archaeological wood ,SEM ,XRD ,FTIR ,nanoindentation ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Huaguangjiao I refers to the ancient Chinese wooden shipwreck of the South Song Dynasty (1127–1279 AD) discovered in the South China Sea in 1996. From 2008 to 2017, the archaeological waterlogged wood was desalted using deionized water combined with ultrasonic treatment, and desalted using EDTA-2Na, EDTAHO, and NaH2PO4·2H2O solutions. In this paper, the degree of degradation of the modified waterlogged archaeological wood and the moisture and content of the main components were determined. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), nanoindentation (NI), and scanning electron microscopy (SEM) were employed to investigate the state of wood degradation after desalination and desulfurization. The results showed that the water content of the wood was as high as 532~1149%, while the basic density was only 0.14~0.18 g/cm3, indicating that the wood had been seriously degraded. The holocellulose content was only 36–40%. Based on the XRD patterns, the degree of cellulose crystallinity in the modified wood was 14.08%. The elastic modulus and hardness of the ancient shipwreck wood after desalination and desulfurization were 1.28–4.31 and 0.10–0.28 GPa, respectively, according to nanoindentation. In addition, the FTIR spectra revealed that the biological deterioration of the modified wood caused cellulose and hemicellulose degradation, but no apparent lignin alteration occurred. The results could provide knowledge for appropriate dewatering, strengthening, and restoration strategies.
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- 2023
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18. Growth, Immunity, and Transcriptome Response to Dietary Wnt/β-Catenin Pathway Activator TWS119 in Penaeus vannamei
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Cuihong Hou, Shougang Song, Lulu Zhu, Lili Shi, Beiping Tan, and Shuang Zhang
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Penaeus vannamei ,TWS119 ,growth performance ,immunity ,transcriptome analysis ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Wnt/β-catenin signalling plays an essential role in the immunity of Penaeus vannamei. In this study, the effects of dietary Wnt/β-catenin pathway activator TWS119 on the growth, immunity, and transcriptome response in P. vannamei were investigated. Penaeus vannamei were fed diets with added TWS119 at doses of 0 (T0), 0.25 (T0.25), 1 (T1), 4 (T4), 16 (T16), or 64 mg·kg−1 (T64), respectively. LvGSK3β activity was effectively inhibited in P. vannamei given TWS119. The growth of P. vannamei in the T16 group was significantly improved when compared with the control group. After Vibrio parahaemolyticus infection, the survival rates (SRs) of P. vannamei in all experimental groups except the T64 group were significantly higher than in the T0 group. Compared with the control group, the immune enzymes’ activities in the serum of P. vannamei increased in all the experimental groups, while the malondialdehyde (MDA) contents decreased. Transcriptome analysis identified 5,073 differentially expressed genes (DEGs) for P. vannamei in the T0 and T16 groups. Most of the DEGs are involved in the ribosome pathway, endocytosis, glycerophospholipid metabolism, Wnt signalling, and FoxO =signalling pathways. The majority of the DEGs were from the ribosome pathway, which is also the most significantly enriched pathway. The study confirmed that the growth and immunity status of P. vannamei could improve by increasing dietary TWS119, which probably regulates the activity of the Wnt/β-catenin pathway and may be closely related to ribosome function and energy metabolism.
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- 2021
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19. GSK3β Plays a Negative Role During White Spot Syndrome Virus (WSSV) Infection by Regulating NF-κB Activity in Shrimp Litopenaeus vannamei
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Shuang Zhang, Lulu Zhu, Cuihong Hou, Hang Yuan, Sheng Yang, Mustafa Abdo Saif Dehwah, and Lili Shi
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glycogen synthase kinase 3β ,nuclear factor κB ,negative regulation ,white spot syndrome virus ,Litopenaeus vannamei ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Glycogen synthase kinase-3 (GSK3), a cytoplasmic serine/threonine-protein kinase involved in a large number of key cellular processes, is a little-known signaling molecule in virus study. In this study, a GSK3 protein which was highly similar to GSK3β homologs from other species in Litopenaeus vannamei (designated as LvGSK3β) was obtained. LvGSK3β was expressed constitutively in the healthy L. vannamei, at the highest level in the intestine and the lowest level in the eyestalk. White spot syndrome virus (WSSV) reduced LvGSK3β expression was in immune tissues including the hemocyte, intestine, gill and hepatopancreas. The inhibition of LvGSK3β resulted in significantly higher survival rates of L. vannamei during WSSV infection than the control group, and significantly lower WSSV viral loads in LvGSK3β-inhibited L. vannamei were observed. Knockdown of LvGSK3β by RNAi resulted in increases in the expression of LvDorsal and several NF-κB driven antimicrobial peptide (AMP) genes (including ALF, PEN and crustin), but a decrease in LvCactus expression. Accordingly, overexpression of LvGSK3β could reduce the promoter activity of LvDorsal and several AMPs, while the promoter activity of LvCactus was increased. Electrophoretic mobility shift assays (EMSA) showed that LvDorsal could bind to the promoter of LvGSK3β. The interaction between LvGSK3β and LvDorsal or LvCactus was confirmed using co-immunoprecipitation (Co-IP) assays. In addition, the expression of LvGSK3β was dramatically reduced by knockdown of LvDorsal. In summary, the results presented in this study indicated that LvGSK3β had a negative effect on L. vannamei by mediating a feedback regulation of the NF-κB pathway when it is infected by WSSV.
- Published
- 2020
- Full Text
- View/download PDF
20. Tyrosine kinase 4 is involved in the reproduction of the platyhelminth parasite Schistosoma japonicum
- Author
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Han Ding, Fengchun Liu, Lulu Zhu, Fei Wu, Quan Liu, Siyu He, Wei Shao, Yinan Du, Cuiping Ren, Jijia Shen, and Miao Liu
- Subjects
Schistosoma japonicum ,Reproduction ,SjTK4 ,Piceatannol ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Schistosomiasis is one of the most common parasitic diseases affecting millions of humans and animals worldwide. Understanding the signal transduction pathways and the molecular basis of reproductive regulation in schistosomes is critically important for developing new strategies for preventing and treating these infections. Syk kinases regulate the proliferation, differentiation, morphogenesis, and survival of various types of cells and have been identified in invertebrates. Tyrosine kinase 4 (TK4), a member of the Syk kinase family, plays a pivotal role in gametogenesis in S. mansoni, affecting the development of the testis and ovaries in this parasite. The role of TK4, however, in the reproduction of S. japonicum is poorly understood. Methods Here, the complete coding sequence of TK4 gene in S. japonicum (SjTK4) was cloned and characterized. The expression of SjTK4 was analyzed at different life-cycle stages and in various tissues of S. japonicum by qPCR. Piceatannol, a Syk kinase inhibitor, was applied to S. japonicum in vitro. The piceatannol-induced morphological changes of the parasites were observed using confocal laser scanning microscopy and the alterations in important egg-shell synthesis-related genes were examined using qPCR analyses. Results SjTK4 mRNA was differentially expressed throughout the life-cycle of S. japonicum. SjTK4 mRNA was highly expressed in the ovary and testis of S. japonicum, with the level of gene expression significantly higher in males than in females. The expression levels of some important egg-shell synthesis related genes were higher in the piceatannol-treated groups than in the vehicle-treated control group and the number of eggs and germ cells also decreased in a concentration-dependent manner. Importantly, large pore-like structures can be found in the testis and ovaries of males and females after treating with piceatannol. Conclusion The results suggest that SjTK4 may play an important role in regulating gametogenesis of S. japonicum. The findings may help better understand the fundamental biology of S. japonicum. Moreover, the effect of S. japonicum treatment by piceatannol provides us with a new idea that inhibition of SjTK4 signaling pathway can effectively retard the development of the testis and ovaries.
- Published
- 2017
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21. CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling
- Author
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Jialei Yang, Wanting He, Lian Gu, Lulu Zhu, Tian Liang, Xueying Liang, Qingqing Zhong, Ruirui Zhang, Aruo Nan, and Li Su
- Subjects
Physiology (medical) ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,General Medicine - Published
- 2023
22. Prospects for Research on New-Form International Chinese Language Textbooks
- Author
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Yi Fu, Chunyan Liu, Shuangyan Guo, Jiayu Shen, and Lulu Zhu
- Abstract
Textbook is one of the “three teachings”, and it is an important basis and carrier of teaching. The new form of textbooks is a product of the intelligent age and an important embodiment of the modernization of teaching materials. In the context of the intelligent era, the development of new forms textbooks is an important content of academic concern. We found that there are not many studies on new forms of international Chinese textbooks, and most of the textbooks have not yet entered the stage of integrated construction. The three-dimensional textbooks such as Great Wall Chinese are still an exception. In this regard, we believe that it is necessary to take the creation of three-dimensional teaching materials as the course, closely follow Chinese Proficiency Grading Standards for International Chinese Language Education, and multi-party construction teams go hand in hand to implement the dynamic update of international Chinese teaching materials resources.
- Published
- 2023
23. Sensitizing Tumors to Immune Checkpoint Blockage via STING Agonists Delivered by Tumor-Penetrating Neutrophil Cytopharmaceuticals
- Author
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Meixi Hao, Lulu Zhu, Siyuan Hou, Sijia Chen, Xiuqi Li, Kaiming Li, Nianci Zhu, Shanshan Chen, Lingjing Xue, Caoyun Ju, and Can Zhang
- Subjects
General Engineering ,General Physics and Astronomy ,General Materials Science - Abstract
Immune checkpoint inhibitors (ICIs) have displayed potential efficacy in triple-negative breast cancer (TNBC) treatment, while only a minority of patients benefit from ICI therapy currently. Although activation of the innate immune stimulator of interferon genes (STING) pathway potentiates antitumor immunity and thus sensitizes tumors to ICIs, the efficient tumor penetration of STING agonists remains critically challenging. Herein, we prepare a tumor-penetrating neotype neutrophil cytopharmaceutical (NEs@STING-Mal-NP) with liposomal STING agonists conjugating on the surface of neutrophils, which is different from the typical neutrophil cytopharmaceutical that loads drugs inside the neutrophils. We show NEs@STING-Mal-NP that inherit the merits of neutrophils including proactive tumor vascular extravasation and tissue penetration significantly boost the tumor penetration of STING agonists. Moreover, the backpacked liposomal STING agonists can be released in response to hyaluronidase rich in the tumor environment, leading to enhanced uptake by tumor-infiltrating immune cells and tumor cells. Thus, NEs@STING-Mal-NP effectively activate the STING pathway and reinvigorate the tumor environment through converting macrophages and neutrophils to antitumor phenotypes, promoting the maturation of dendritic cells, and enhancing the infiltration and tumoricidal ability of T cells. Specifically, this cytopharmaceutical displays a significant inhibition on tumor growth and prolongs the survival of TNBC-bearing mice when combined with ICIs. We demonstrate that neutrophils serve as promising vehicles for delivering STING agonists throughout solid tumors and the developed neutrophil cytopharmaceuticals with backpacked STING agonists exhibit huge potential in boosting the immunotherapy of ICIs.
- Published
- 2023
24. CircUSP36 attenuates ischemic stroke injury through the miR-139-3p/SMAD3/Bcl2 signal axis
- Author
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Jialei Yang, Wanting He, Lian Gu, Jianxiong Long, Lulu Zhu, Ruirui Zhang, Zhi Zhao, Bingyi Xu, Aruo Nan, and Li Su
- Subjects
Mice ,MicroRNAs ,Proto-Oncogene Proteins c-bcl-2 ,Reperfusion Injury ,Animals ,Humans ,Apoptosis ,RNA, Circular ,Smad3 Protein ,General Medicine ,Ischemic Stroke - Abstract
Circular RNAs (circRNAs) play important roles in a variety of physiological and pathological processes. Researches demonstrated that circRNAs provided novel strategies for the prevention and treatment of IS. However, the biological function of hsa_circ_0045932 (circUSP36) has not been revealed yet. Here, we explored the effect of circUSP36 on IS and its mechanism. In the present study, we found that circUSP36 expression was significantly decreased in the peripheral blood of IS patients and was negatively correlated with the severity, infarct volume and poor prognosis of IS. Functionally, circUSP36 silencing inhibited cellular activity and proliferation and promoted apoptosis after oxygen-glucose deprivation/reperfusion (OGD/R) treatment, while circUSP36 overexpression reversed these cellular phenotypes in vitro. Adeno-associated virus (AAV)-mediated overexpression of circUSP36 attenuates brain injury and neurological deficit and promotes motor function recovery of transient middle cerebral artery occlusion (tMCAO) mice. Subsequently, the RNA antisense purification (RAP) and luciferase reporter assay confirmed that circUSP36 acts as a sponge to adsorb miR-139-3p, and miR-139-3p could bind and inhibit SMAD3 expression. Further rescue experiments showed that both miR-139-3p overexpression and SMAD3 silencing could abolish the antiapoptotic effect of circUSP36. In summary, we reveal for the first time that circUSP36 attenuates ischemic stroke injury through the miR-139-3p/SMAD3/Bcl2 signal axis, which make circUSP36 a potential therapeutic target for IS.
- Published
- 2022
25. A Potential Immune-Related miRNAs Regulatory Network and Corresponding Diagnostic Efficacy in Schizophrenia
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Fangping Liao, Qingqing Zhong, Xueying Liang, Wanshen Zhao, Tian Liang, Lulu Zhu, Tongshun Li, Jianxiong Long, and Li Su
- Subjects
Cellular and Molecular Neuroscience ,General Medicine ,Biochemistry - Published
- 2023
26. Spencerozyma pingqiaoensis sp. nov., a yeast species isolated from the external surface of rice leaves in China
- Author
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Chunyue Chai, Lulu Zhu, Jinli Liu, Xuemei Han, Pumin Nutaratat, Pannida Khuuamwong, and Fengli Hui
- Subjects
General Medicine ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Abstract
Four strains (NYNU 178247, NYNU 178251, DMKU-PAL160 and DMKU-PAL137) representing a novel yeast species were isolated from the external surfaces of rice and pineapple leaves collected in China and Thailand. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit rRNA gene revealed that the novel species belonged to the genus Spencerozyma. The D1/D2 sequence of the novel species differed from its closest relative, Spencerozyma acididurans SYSU-17T, by 3.2 % sequence divergence. The species also differed from Spencerozyma crocea CBS 2029T and Spencerozyma siamensis DMKU13-2T, by 3.0–6.9 % sequence divergence in the D1/D2 sequences out of 592 bp. In the ITS regions, the novel species displayed 19.8–29.2% sequence divergence from S. acididurans SYSU-17T, S. crocea CBS 2029T and S. siamensis DMKU13-2T out of 655 bp. Furthermore, the novel species could also be differentiated from the closely related species by some physiological characteristics. The species name of Spencerozyma pingqiaoensis sp. nov. (Holotype CBS 15238, Mycobank MB 844734) is proposed to accommodate these four strains.
- Published
- 2023
27. Genome Sequence Resource of Albifimbria verrucaria Causing the Leaf Spot Disease of the Spinach Plant Spinacia oleracea
- Author
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Chunyue Chai, Chongyi Chen, Lulu Zhu, Jinli Liu, and Fengli Hui
- Subjects
Plant Science ,Agronomy and Crop Science - Published
- 2022
28. Genome-wide association studies-supported rs12966547 variant of the long noncoding RNA LOC105372125 is significantly associated with susceptibility to schizophrenia and bipolar disorder in Han Chinese women
- Author
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Zhi Zhao, Lulu Zhu, Xulong Wu, Qiang Chen, Bingyi Xu, Jialei Yang, Xiaojing Guo, and Li Su
- Subjects
Male ,China ,Psychiatry and Mental health ,Bipolar Disorder ,Schizophrenia ,Genetics ,Humans ,Female ,RNA, Long Noncoding ,Biological Psychiatry ,Genetics (clinical) ,Genome-Wide Association Study - Abstract
Genome-wide association studies have found that rs12966547 polymorphism was associated with susceptibility to schizophrenia in European populations. Recent studies showed that a genetic overlap may exist in schizophrenia and bipolar disorder. Here, we analyzed the associations between LOC105372125 rs12966547 polymorphism and schizophrenia and bipolar disorder in the Han Chinese population.Our study recruited 548 schizophrenia patients, 512 bipolar disorder patients, and 598 healthy controls. Genotyping of rs12966547 were performed using the Sequenom MassARRAY platform.A significant association between rs12966547 polymorphism and susceptibility to bipolar disorder was observed after adjusting for sex and age (additive model: Padj = 0.040, recessive model: Padj = 0.044). However, no significant association was found between rs12966547 polymorphism and schizophrenia risk (all P0.05). In the analysis of gender, rs12966547 polymorphism was significantly associated with bipolar disorder (additive model: Padj = 0.027) and schizophrenia (dominant model: Padj = 0.039) in women. However, no significant association was found between rs12966547 polymorphism and the risk of bipolar disorder or schizophrenia in men (all Padj0.05).Polymorphism of rs12966547 on the long noncoding RNA LOC10537215 are a shared genetic variant of schizophrenia and bipolar disorder in Han Chinese women.
- Published
- 2022
29. Analysis of expression profiles and bioinformatics suggests that plasma exosomal circular RNAs may be involved in ischemic stroke in the Chinese Han population
- Author
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Bingyi Xu, Xianli Huang, Yan Yan, Zhi Zhao, Jialei Yang, Lulu Zhu, Yibing Yang, Baoyun Liang, Lian Gu, and Li Su
- Subjects
China ,MicroRNAs ,Cellular and Molecular Neuroscience ,Computational Biology ,Humans ,RNA, Circular ,Neurology (clinical) ,Biochemistry ,Ischemic Stroke - Abstract
Circular RNAs (circRNAs) have been confirmed to be associated with ischemic stroke(IS), but the involvement of exosomal circRNAs in plasma still needs to be extensively discussed. Therefore, we aimed to investigate the expression profile of exosomal circRNAs in plasma and the potential roles and mechanisms of exosomal circRNAs in the pathogenesis of ischemic stroke in the Chinese Han population. In this study, the plasma exosomal circRNA expression profiles of three IS patients and three healthy controls were analyzed using circRNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and circRNA-miRNA-mRNA regulatory network analysis were performed for the aberrantly expressed genes. Protein-protein interaction (PPI) networks and molecular complex detection algorithms (MCODEs) were analyzed by STRING and Cystoscope for functional annotation and construction, respectively. RNA-Seq analysis revealed that a total of 3540 circRNAs were aberrantly expressed in exosomes, 1177 circRNAs were significantly upregulated, and 2363 circRNAs were downregulated in IS patients compared to healthy controls. Bioinformatics analysis revealed that the parental genes of differentially expressed circRNAs as well as the mRNAs predicted in the circRNA-miRNA-mRNA regulatory network are enriched for signaling pathways associated with IS pathology, such as the MAPK signaling pathway, lipid and atherosclerosis, neurotrophic factor signaling pathways, mTOR signaling pathway, the p53 signaling pathway etc. Then, 10 hub genes were identified from the PPI and module networks, including FBXW11, FBXW7, UBE2V2, ANAPC7, CDC27, UBC, CDC5L, POLR2H, POLR2F and RBX1. Overall, the present study provides evidence of an altered plasma exosomal circRNA expression profile and its potential function in IS. Our findings may contribute to the study of the pathogenesis of circRNAs in IS and provide ideas for studying potential diagnostic biomarkers and therapeutic targets for IS.
- Published
- 2022
30. Coal Mining Activities Driving the Changes in Microbial Community and Hydrochemical Characteristics of Underground Mine Water
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Li Zhang, Zhimin Xu, Yajun Sun, Yating Gao, and Lulu Zhu
- Subjects
China ,Coal ,coal mining impacts ,hydrochemical characteristics ,microbial communities ,zone-specific patterns ,biogeochemical process ,Sulfates ,Health, Toxicology and Mutagenesis ,Microbiota ,Public Health, Environmental and Occupational Health ,Coal Mining ,Groundwater ,Water Pollutants, Chemical ,Sulfur ,Mining ,Environmental Monitoring - Abstract
Coal mining can cause groundwater pollution, and microorganism may reflect/affect its hydrochemical characteristics, yet little is known about the microorganism’s distribution characteristics and its influence on the formation and evolution of mine water quality in underground coal mines. Here, we investigated the hydrochemical characteristics and microbial communities of six typical zones in a typical North China coalfield. The results showed that hydrochemical compositions and microbial communities of the water samples displayed apparent zone-specific patterns. The microbial community diversity of the six zones followed the order of surface waters > coal roadways > water sumps ≈ rock roadways ≈ goafs > groundwater aquifers. The microbial communities corresponded to the redox sensitive indices’ levels. Coal roadways and goafs were the critical zones of groundwater pollution prevention and control. During tunneling in the panel, pyrite was oxidized by sulfur-oxidizing bacteria leading to SO42− increase. With the closure of the panel and formation of the goaf, SO42− increased rapidly for a short period. However, with the time since goaf closure, sulfate-reducing bacteria (e.g., c_Thermodesulfovibrionia, Desulfobacterium_catecholicum, etc.) proportion increased significantly, leading to SO42− concentration’s decrease by 42% over 12 years, indicating the long-term closed goafs had a certain self-purification ability. These findings would benefit mine water pollution prevention and control by district.
- Published
- 2022
31. CPN Tools' Application in Verification of Parallel Programs.
- Author
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Lulu Zhu, Weiqin Tong, and Bin Cheng
- Published
- 2010
- Full Text
- View/download PDF
32. LncRNA SERPINB9P1 expression and polymorphisms are associated with ischemic stroke in a Chinese Han population
- Author
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Zhi Zhao, Jialei Yang, Xulong Wu, Huang Jiao, Bingyi Xu, Xinyi Zhao, Lulu Zhu, Lian Gu, and Li Su
- Subjects
Oncology ,China ,medicine.medical_specialty ,Neurology ,Dermatology ,Severe stroke ,Polymorphism, Single Nucleotide ,Brain Ischemia ,Pathogenesis ,Chinese han population ,Polymorphism (computer science) ,Internal medicine ,medicine ,Humans ,In patient ,Ischemic Stroke ,business.industry ,General Medicine ,Stroke ,MicroRNAs ,Psychiatry and Mental health ,Ischemic stroke ,Female ,RNA, Long Noncoding ,Neurology (clinical) ,business ,Neurological impairment - Abstract
Long noncoding RNAs (lncRNAs) were reported to play important roles in the pathogenesis of ischemic stroke (IS). Our study aimed to investigate the role of lncRNA SERPINB9P1 expression in ischemic stroke and the association between SERPINB9P1 polymorphisms and IS risk, as well as examine the correlation of SERPINB9P1 expression and variants with clinical parameters of IS. The SERPINB9P1 levels in human participants and oxygen-glucose deprivation (OGD)-treated human A172 cells were measured by qRT-PCR. The SERPINB9P1 polymorphisms (rs375556 and rs318429) were genotyped by the MassARRAY platform. We found that the SERPINB9P1 expression was significantly downregulated in patients with IS compared with that in healthy controls. On the 14th day in the hospital, the SERPINB9P1 level in patients with moderate and severe stroke was significantly downregulated compared with the normal group. After stratification by gender, the rs375556 polymorphism was significantly associated with susceptibility to female IS in the recessive model, and the significant association remained after adjusting for age. After adjusting for gender and age, rs318429 was significantly associated with FPG and D-D levels, and rs375556 was significantly associated with INR and PTA levels in IS cases. Besides, the lncRNA SERPINB9P1 expressed downregulated in OGD/reoxygenation-treated human A172 cells. In conclusion, the lncRNA SERPINB9P1 may protect against cerebral ischemia-reperfusion injury and neurological impairment after IS. The SERPINB9P1 rs375556 polymorphism was associated with susceptibility to female IS, and SERPINB9P1 polymorphisms may influence the metabolism of blood glucose and regulation of coagulation function in patients with IS.
- Published
- 2021
33. Construction of a Bioinspired Hierarchical BiVO4/BiOCl Heterojunction and Its Enhanced Photocatalytic Activity for Phenol Degradation
- Author
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Jian Pan, Jingyi Lin, Wang Zhang, Lulu Zhu, Fang Song, Shenmin Zhu, Siyuan Liu, Qian Su, Mingrui Zhang, and Yao Li
- Subjects
chemistry.chemical_compound ,Materials science ,Reaction rate constant ,chemistry ,Chemical engineering ,Specific surface area ,Photocatalysis ,Substrate (chemistry) ,Phenol ,Degradation (geology) ,General Materials Science ,Heterojunction ,Visible spectrum - Abstract
Development of a p-n heterojunction to achieve efficient degradation of organic pollutants is a promising approach in the field of photocatalysis. Herein, BiVO4 with bioinspired hierarchical structures was prepared with the sol-gel method and combined with BiOCl nanoplates to construct a 3D/2D configuration via an in situ deposition route. The hierarchical BiVO4 served as an excellent substrate to achieve the uniform loading of BiOCl nanoplates. The obtained 3D/2D BiVO4/BiOCl hybrids exhibited significantly enhanced photocatalytic efficiency for degrading phenol under visible light irradiation, with a first-order reaction rate constant that was 9.9 and 1.9 times higher than those of hierarchical BiVO4 and the BiVO4/BiOCl hybrids without hierarchical structures, respectively. Moreover, the hierarchical BiVO4/BiOCl also displayed good photochemical stability for the degradation of phenol after three recycles. The p-n heterojunction and hierarchical structure worked together to form a spatial conductive network framework, which possessed improved visible light absorption, high specific surface area, as well as effective separation and transfer of photogenerated charge carriers.
- Published
- 2021
34. Application of machine learning in diagnostic value of mRNAs for bipolar disorder
- Author
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Jialei Yang, Xulong Wu, Bingyi Xu, Zhi Zhao, Lulu Zhu, Jianxiong Long, and Li Su
- Subjects
Bipolar Disorder ,Support Vector Machine ,Artificial neural network ,business.industry ,Value (computer science) ,Mental illness ,medicine.disease ,Machine learning ,computer.software_genre ,Machine Learning ,Support vector machine ,Psychiatry and Mental health ,FYN ,medicine ,Humans ,RNA, Messenger ,Bipolar disorder ,Artificial intelligence ,Extreme gradient boosting ,medicine.symptom ,business ,Mania ,computer - Abstract
Purpose Bipolar disorder (BD) is a type of severe mental illness with symptoms of mania or depression, it is necessary to find out effective diagnostic biomarkers for BD due to diagnosing BD is based on clinical interviews without objective indicators. Materials and methods The mRNA expression levels of genes included PIK3R1, FYN, TP53, PRKCZ, PRKCB, and YWHAB in the peripheral blood of 43 patients with bipolar disorder and 47 healthy controls were detected. Machine learning methods included Artificial Neural Networks, Extreme Gradient Boosting, Random Forest, and Support Vector Machine were adopted to fit different gene combinations to evaluate diagnostic value for bipolar disorder. Results The combination 'PIK3R1 + FYN' in the SVM model showed the best diagnostic value, with AUC, sensitivity, and specificity values of 0.951, 0.928, and 0.937, respectively. Conclusions The diagnostic efficiency for bipolar disorder was significantly improved by fitting PIK3R1 and FYN through the Support Vector Machine model.
- Published
- 2021
35. Tuning the Active Sites of Atomically Thin Defective Bi12O17Cl2 via Incorporation of Subnanometer Clusters
- Author
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Fan Dong, Lulu Zhu, Bin Jiang, Jiexiang Xia, Sujuan Wu, and Yifan Wu
- Subjects
Reaction mechanism ,Materials science ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Redox ,0104 chemical sciences ,Photocatalysis ,No removal ,General Materials Science ,0210 nano-technology ,Selectivity - Abstract
The introduction of subnanometer clusters as active sites on the surface of photocatalysts for efficiently tuning the selectivity and activity of the photocatalyts is still a challenge. Herein, the subnanometer Ag/AgCl clusters were incorporated on atomically thin defective Bi12O17Cl2 nanosheets via rebinding with unsaturated Cl atoms. Benefiting from the surficial Bi vacancies (VBi) and Bi-O vacancies (VBi-O) in this atomically thin architecture, the local atomic arrangement was tuned so that the subnanometer Ag/AgCl clusters were successfully incorporated. An enhancement of photocatalytic activity for NO removal was achieved in which the activity is 3 times higher than that of Bi12O17Cl2 and 1.8 times higher than that of defective Bi12O17Cl2. The substitution of the active sites from surficial VBi and VBi-O to be subnanometer Ag/AgCl clusters enables a tunable redox potential and different reaction mechanisms in NO removal. Moreover, the selectivity of the photoinduced redox reaction on NO oxidation and CO2 reduction was achieved via introducing an extra energy level.
- Published
- 2021
36. Comprehensive Analysis of Blood-Based m6A Methylation in Human Ischemic Stroke
- Author
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Lulu Zhu, Shengying Liu, Fangping Liao, Jialei Yang, Tian Liang, Yibing Yang, Xianli Huang, Lian Gu, and Li Su
- Subjects
Cellular and Molecular Neuroscience ,Neurology ,Neuroscience (miscellaneous) - Abstract
Alterations of N6-methyladenosine (m6A) methylation have been reported in the cerebral cortices of mouse and rat models of ischemic stroke (IS). However, the role of m6A methylation in human IS is still unknown. We assessed m6A levels in peripheral blood from patients with IS and healthy controls. A transient middle cerebral artery occlusion and reperfusion (tMCAO/R) mouse model, and an oxygen-glucose deprivation/reperfusion (OGD/R) model in A172 cells were established to further assess m6A levels. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing were performed in the peripheral blood of patients with IS and healthy controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were used to identify underlying biological processes. In this study, we found that global m6A levels were elevated in the peripheral blood of patients with IS, in the cerebral cortex of mice after tMCAO/R treatment and in A172 cells after OGD/R treatment. MeRIP-seq analysis identified 2115 altered m6A peaks in patients with IS, 1052 upregulated and 1063 downregulated. Downregulated methylated mRNAs were enriched in Hippo signaling pathway, cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, etc. Upregulated methylated mRNAs were enriched in calcium signaling pathways, Hedgehog signaling pathway, MAPK signaling pathway, etc. Moreover, a total of 84 differentially expressed mRNAs with altered m6A peaks were identified and enriched in EGFR tyrosine kinase inhibitor, Hematopoietic cell lineage, and cytokine-cytokine receptor interactions. This study is the first to profile the transcriptome-wide m6A methylome of peripheral blood in human IS and uncover increased global m6A levels in the peripheral blood of patients with IS.
- Published
- 2022
37. Hopf bifurcation for a differential-algebraic biological economic system with time delay.
- Author
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Guodong Zhang 0001, Boshan Chen, Lulu Zhu, and Yi Shen 0002
- Published
- 2012
- Full Text
- View/download PDF
38. Hopf bifurcation and stability for a differential-algebraic biological economic system.
- Author
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Guodong Zhang 0001, Lulu Zhu, and Boshan Chen
- Published
- 2010
- Full Text
- View/download PDF
39. Preparation of high oil absorption microfiber cryogels by mechanical method
- Author
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Huamin Zhai, Haonan Zhang, Lulu Zhu, Jin Zhao, Shiwen Xue, and Hao Ren
- Subjects
business.product_category ,Materials science ,Pulp (paper) ,Forestry ,Plant Science ,engineering.material ,Raw material ,Industrial and Manufacturing Engineering ,chemistry.chemical_compound ,Adsorption ,Chemical engineering ,chemistry ,Transmission electron microscopy ,Microfiber ,Dispersion stability ,Particle-size distribution ,engineering ,Lignin ,General Materials Science ,business - Abstract
Poplar alkaline peroxide mechanical pulp (APMP) was used as plant fiber raw material, and it was graded according to the lignin content. The graded raw materials were used to prepare lignin-containing microfibers (LCMFs) by micro-grinding, and then, the lignin-containing microfiber cryogels (LCMF-cryogels) were prepared by vacuum freeze-drying. The particle size distribution, micromorphology and dispersion stability of LCMFs were characterized by laser diffraction particle size analyzer, transmission electron microscopy and Zeta potentiometer. Then, the crystalline structure, pore structure, surface morphology and adsorption properties of LCMF-cryogels were analyzed. In addition, LCMF-cryogels were compared with the microfiber cryogels (MF-cryogels) which use commercial eucalyptus chemical bleaching pulp as raw material. It was proved that using poplar APMP as raw material to prepare cryogels is more advantageous. The results showed that the lower the lignin content in plant materials, the better the oil adsorption ability of cryogels. This is because the binding effect of lignin on the fiber is reduced, which makes the fibrillation of the fiber easier by external mechanical action. The maximum adsorption capacity of LCMF-cryogel for methyl silicone oil is 215 g/g, while that of MF-cryogel prepared from commercial eucalyptus chemical bleaching pulp is 102.5 g/g. The analysis of the properties of raw fiber and the gel pores shows that the change in the fiber morphology caused by different pulping methods has important influence on the development of LCMF-cryogels by mechanical refining. Poplar APMP is more suitable for preparing fiber-based cryogels oil-absorbing materials than commercial eucalyptus chemical bleaching pulp.
- Published
- 2020
40. Rationality and coordination: Visualizing highway network in Sichuan, China
- Author
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Sijing Liu, Wenjie Sun, LuLu Zhu, and Guoqi Li
- Subjects
Network on ,Geography, Planning and Development ,Economics ,Regional science ,Rationality ,Environmental Science (miscellaneous) ,China ,Cartogram ,Regional differences ,Effective solution - Abstract
The highway network is considered to be an effective solution for reducing regional differences and promoting high-quality economic development. This study mapped Sichuan’s highway network on an equal per capita GDP cartogram and on an equal highway freight volumes cartogram, which can provide a unique perspective to recognize the rationality of highway layouts and its coordination relationship with the population and economic development. The research result indicates that highway layouts are seriously affected by the topographical and geologic conditions, and the population and level of economic development are key influencing factors. It also shows that the density of the highway network is still seriously inadequate in the vast mountain and plateau area.
- Published
- 2020
41. Whole Transcriptome Sequencing Identified CircRNA Profiles and the Related Networks in Schizophrenia
- Author
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Fangping Liao, Lulu Zhu, Jialei Yang, Xulong Wu, Zhi Zhao, Bingyi Xu, Qingqing Zhong, Zheng Wen, Jianxiong Long, and Li Su
- Subjects
RNA-Binding Proteins ,General Medicine ,RNA, Circular ,Cellular and Molecular Neuroscience ,Fragile X Mental Retardation Protein ,MicroRNAs ,Phosphatidylinositol 3-Kinases ,Ubiquitin-Conjugating Enzymes ,Exome Sequencing ,Schizophrenia ,Humans ,Gene Regulatory Networks ,RNA, Messenger ,Transcriptome - Abstract
Schizophrenia (SCZ) is a complex psychiatric syndrome with uncertain etiology. This study aimed to uncover the expression profiles and related regulatory networks of circular RNA (circRNA) in SCZ. Whole transcriptome sequencing was performed to assess the expression profiles of circRNAs and microRNAs (miRNAs) in the peripheral blood of three patients with SCZ and three healthy controls. Five circRNAs were validated by quantitative real-time PCR (RT-qPCR). TargetScan, RNAhybrid, and miRanda were performed to predict the target miRNAs of the top 10 dysregulated circRNAs. MiRTarBase was applied to predict the target mRNAs of miRNAs to construct circRNA-miRNA-mRNA (ceRNA) networks. CatRAPID and StarBase were used to predict the target RNA-binding proteins (RBPs) of circRNAs to construct circRNA-RBP networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to predict the potential functions of the maternal genes of circRNAs and target mRNAs. In total, 450 circRNAs and 160 miRNAs were found to be significantly differentially expressed, with hsa_circ_0003999 and hsa_circ_0030042 being significantly different between patients with SCZ and healthy controls (P 0.05). The PI3K-AKT, MAPK, and cell cycle pathways were predicted to be associated with SCZ. GO analysis showed that focal adhesion was related to SCZ. The ceRNA networks, especially hsa_circ_0006151/hsa-miR-4685-3p/ZBTB16, hsa_circ_0000008/hsa-miR-1976/ZBTB16, and the hsa_circ_0007963/hsa-miR-3127-3p/UBE2K axes have the greatest probability of being involved in the pathophysiology of SCZ. The RBP networks, FXR1, FXR2, DGCR8, XRN2, FMR1, and QKI were the RBPs associated with SCZ. In conclusion, the circRNAs, ceRNAs, and RBP network expression patterns and related pathways indicate the potential role of circRNAs in the pathogenesis and development of SCZ.
- Published
- 2022
42. Carbon emission effects of publicly planned logistics nodes: experience from Chengdu, China
- Author
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Wenjie Sun, Jin Zhang, Guoqi Li, Lulu Zhu, and Nannan He
- Subjects
Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Abstract
Both academics and policymakers regard publicly planned logistics nodes (PPLN) as a potential measure to promote logistics agglomeration and mitigate environmental damage caused by logistics activities. However, such measure may lead to non-linear carbon emission effect from the perspective of agglomeration economy, and empirical evidences are yet to be found to verify this inference. This paper uses DID method and mediating effect models to explore the effects and mechanisms of PPLN on CO
- Published
- 2022
43. Retention Performance of Al2o3 Plot Column Coated with Γ–Alumina Nanoparticles in the Highly Volatile Hydrocarbons Separation
- Author
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Zhonglai Wang, Chengying Du, and Lulu Zhu
- Published
- 2022
44. Retention performance of alumina porous layer open-tubular column coated with γ–alumina nanoparticles in the highly volatile hydrocarbons separation
- Author
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Zongping, Jia, Chengying, Du, Lulu, Zhu, and Zhonglai, Wang
- Subjects
Organic Chemistry ,General Medicine ,Biochemistry ,Analytical Chemistry - Abstract
An alumina porous layer open-tubular (Al
- Published
- 2023
45. 5’UTR Variant rs6017916 Regulates SRC Expression and Contributes to the Susceptibility to Ischemic Stroke in the Chinese Han Population
- Author
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Jialei Yang, Xiaoxiao Song, Yibing Yang, Yan Yan, Baoyun Liang, Lulu Zhu, Lian Gu, and Li Su
- Abstract
Previous studies reported that the SRC protein was involved in a variety of pathological mechanisms related to ischemic stroke (IS). In this study, we conducted a genetic association study between rs6017916 within the 5’UTR region of SRC gene and IS susceptibility. A total of 533 IS patients and 531 healthy controls were recruited to participate in the current study. The sequenom MassARRAY technology platform was used for genotyping. The quantitative polymerase chain reaction (qPCR) was conducted to detect SRC mRNA expression. The dual luciferase reporter system was used to verify the regulation of rs6017916 on SRC mRNA expression. Results showed that SRC mRNA expression was significantly increased in IS patients than that in controls (PSRC mRNA expression differentiated between controls and IS patients with an area under the curve (AUC) of 0.935 corresponding to a specificity of 0.820 and sensitivity of 0.920. Genetic association analysis showed that rs6017916 was significantly associated with IS susceptibility under multiple genetic models, including additive [OR (95% CI)=0.76 (0.60,0.96), Padj=0.019] and dominant [OR (95% CI)=0.75(0.58,0.98), Padj=0.031]. In addition, the dual luciferase reporter system showed that the minor allele C of rs6017916 inhibited luciferase activity compared with the major allele A. In summary, we report that SRC mRNA expression was significantly increased in IS patients and was a potential diagnostic biomarker. Moreover, the 5’UTR variant rs6017916 of SRC was significantly associated with IS susceptibility. And rs6017916 might affect the pathological process of IS by regulating SRC expression.
- Published
- 2021
46. Contribution of secondary bonds to the storage stability of ready-to-eat sea cucumber
- Author
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Qiaoji Tian, Lin Lin, Xin Qi, Lulu Zhu, Li Hao, Ling Wu, Jinhua Wang, and Hu Hou
- Subjects
Hardness ,Sea Cucumbers ,Animals ,Water ,Hydrogen Bonding ,General Medicine ,Collagen ,Food Science ,Analytical Chemistry - Abstract
To explore the contribution of secondary bonds on storage stability of RSC, urea, n-propanol and sodium nitrite (NaNO
- Published
- 2021
47. The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells
- Author
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Jie Liu, Zhenxing Ren, Lin Yang, Lulu Zhu, Yi li, Caiqun Bie, Helu Liu, Yichun Ji, Dongfeng Chen, Meiling Zhu, and Weihong Kuang
- Subjects
Cancer Research ,Cellular and Molecular Neuroscience ,Immunology ,Cell Biology - Abstract
Ferroptosis is a type of cell death induced by the iron-dependent accumulation of lipid hydroperoxides and reactive oxygen species (ROS) in cells. Inhibiting ferroptosis is important for improving the survival of transplanted bone marrow-derived mesenchymal stem cells (BMSCs). Although it is known that NOP2/Sun RNA methyltransferase 5 (NSUN5) post-transcriptionally regulates ferroptosis in BMSCs through RNA methylation, the precise mechanisms underlying these effects have not been reported. In this study, we demonstrate that NSUN5 is downregulated in erastin-induced ferroptosis in BMSCs. Ferroptosis was inhibited by the overexpression ofNSUN5or ferritin heavy chain/light-chain (FTH1/FTL) and was enhanced byNSUN5knockdown. RNA immunoprecipitation experiments revealed that NSUN5 binds to FTH1/FTL, whileNSUN5depletion reduced the levels of 5-methylcytosine inFTH1/FTLRNA and increased intracellular iron concentrations, resulting in the downregulation of glutathione peroxidase 4 (GPX4) and the accumulation of ROS and lipid peroxidation products. Co-immunoprecipitation experiments demonstrated that the recognition ofFTH1andFTLby NSUN5 is dependent on the recruitment of tumor necrosis factor receptor-associated protein 1 (TRAP1). These results suggested that the NSUN5-FTH1/FTL pathway mediates ferroptosis in BMSCs and that the therapeutic targeting of components of this pathway may promote resistance to ferroptosis and improve the survival of transplanted BMSCs.
- Published
- 2021
48. Effect of gallic acid and chlorogenic acid on physicochemical, microstructural and thermal degradation properties of ready-to-eat sea cucumber
- Author
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Lulu Zhu, Xiao Sun, Yan Fan, Yangfan Wang, Xin Qi, and Hu Hou
- Subjects
Gallic Acid ,Sea Cucumbers ,Temperature ,Animals ,Water ,General Medicine ,Chlorogenic Acid ,Food Science ,Analytical Chemistry - Abstract
To enhance thermal stability of ready-to-eat sea cucumbers (RSC), gallic acid (GA) and chlorogenic acid (CA) were employed. Physicochemical and microstructural properties of RSC were evaluated. When RSC were treated by 12 mg/mL of GA and 10 mg/mL of CA, ε-amino group level decreased by 50.2% and 47.9%, and the hardness increased by 74.8% and 80.0%, respectively. The proportion of free water decreased by 10.9% and 8.8%, and the relaxation time decreased by 52.9 and 57.8 ms, respectively. In addition, aggregation and density of collagen fibers increased and their porosity decreased, observed by Van Gieson's staining and scanning electron microscope images. After crosslinked by 12 mg/mL GA and 10 mg/mL CA, the peak temperatures increased to 137.5 °C and 130.0 °C similar to changes of activation energy, indicating that the thermal stability of RSC was improved. It can provide a theoretical basis for prolonging the storage life of RSC.
- Published
- 2021
49. Construction of a Bioinspired Hierarchical BiVO
- Author
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Qian, Su, Lulu, Zhu, Mingrui, Zhang, Yao, Li, Siyuan, Liu, Jingyi, Lin, Fang, Song, Wang, Zhang, Shenmin, Zhu, and Jian, Pan
- Abstract
Development of a p-n heterojunction to achieve efficient degradation of organic pollutants is a promising approach in the field of photocatalysis. Herein, BiVO
- Published
- 2021
50. Structural analysis of poplar and Masson pine lignocresols and comparison of their bovine serum albumin adsorption characteristics
- Author
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Zhang Feng, Dongliang Xu, Guigan Fang, Hao Ren, and Lulu Zhu
- Subjects
Chromatography ,biology ,Chemistry ,Mechanical Engineering ,General Chemical Engineering ,fungi ,010401 analytical chemistry ,food and beverages ,Masson pine ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,complex mixtures ,01 natural sciences ,0104 chemical sciences ,Adsorption ,Media Technology ,biology.protein ,General Materials Science ,Bovine serum albumin ,0210 nano-technology - Abstract
In this study, poplar and Masson pine trees were used as raw materials to examine the structural characteristics of lignin that had been isolated with a phase separation system. Following phase separation, the structure of lignocresol (LC) was characterized with Fourier transform infrared (FTIR) spectroscopy, high-performance gel permeation chromatography (GPC), proton nuclear magnetic resonance (1H-NMR) spectroscopy, and two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance (2D HSQC NMR) spectroscopy. Phase separation made it possible to analyze the proportions and the differences between various structural units in the isolated lignin, as well as the types of linkages between them. In-situ and real-time monitoring of the adsorption and desorption properties of the LC membranes were conducted with a bovine serum albumin (BSA) assay and a quartz crystal microbalance (QCM-D). Our findings revealed that the LC membrane of Masson pine adsorbed more BSA than that of poplar, indicating Masson pine LC molecules were more hydrophobic due to a higher proportion of guaiacyl (G)-type lignin units.
- Published
- 2019
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