1. Mid-infrared spectroscopy of earthworm bodies to investigate their species belonging and their relationship with the soil they inhabit
- Author
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Céline Pelosi, Hanane Aroui Boukbida, Yvan Capowiez, Cornelia Rumpel, Pascal Jouquet, Thao Pham, Anne Pando, Nicolas Bottinelli, Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Soils and Fertilizers Research Institute (SFRI), Vietnam Academy of Agricultural Sciences (VAAS), University of Science and Technology of Hanoi (USTH) through the Mater 2 internship grant, Federation Ile-deFrance for Research on the Environment (FIRE), and Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0106 biological sciences ,Biogeochemical cycle ,[SDV]Life Sciences [q-bio] ,Soil Science ,FRANCE ,[SDU.STU]Sciences of the Universe [physics]/Earth Sciences ,01 natural sciences ,Feeding behavior ,Botany ,Ecological category ,Lumbricid species ,Incubation ,Ecology ,biology ,Earthworm ,Allolobophora chlorotica ,04 agricultural and veterinary sciences ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,Habitat ,Soil water ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Composition (visual arts) ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Lumbricus terrestris ,010606 plant biology & botany - Abstract
Interactions of earthworm species with their environment and in particular the soil they inhabit is poorly understood. In this study, we aimed to use mid infrared absorption (MIR) spectroscopy of earthworm bodies in combination with discriminant analysis of principal components (DAPC), in order to investigate if their biogeochemical composition is determined by their species belonging or the soil they inhabit. Our conceptual approach included the comparison of MIR spectra of four earthworm species (Lumbricus terrestris, Allolobophora chlorotica, Aporrectodea caliginosa and Aporrectodea icterica) sampled from four different agricultural fields in France. Moreover, we tested the influence of the habitat on the earthworm MIR spectra by exchanging individuals and soils in an incubation experiment. MIR spectra varied according to species and sampling site. The MIR spectra of earthworm bodies also changed during the incubation experiment, and for L. terrestris, A. chlorotica and A. icterica depended on the properties of the soil ingested. This suggested that the food source in different habitats impacted the biogeochemical composition of earthworm bodies. DAPC allowed for the identification of earthworm species ranging from 64 to 100% of accuracy (mean 78%). We thus conclude that MIR spectroscopy may be a suitable approach to identify earthworm species, but that their signature may also allow to investigate their ecology as determined by the soil they inhabit.
- Published
- 2021