Search

Amorphous assembling of SiO 2 colloidal spheres is a convenient strategy to obtain amorphous photonic structure, such structure, referred to as SiO 2 amorphous colloidal arrays (ACAs), can demonstrate angle-independent structural color owing to constructive interference. To date, an empirical equation for materials researchers to design the color of SiO 2 ACAs remains vacant, and several influence factors concerning with the color of SiO 2 ACAs are still ignored and uninvestigated. Herein, based on single scattering model, we have proposed an empirical equation to predict the reflection peak of SiO 2 ACAs to design their color, and influence factors including background and thickness on the color of SiO 2 ACAs were also investigated. By preparing spheres within the designed range as building blocks, SiO 2 ACAs with colors varying from blue to red were achieved whose reflection peaks position fits well with the empirical equation. This study can help materials researchers to design and manipulate structural color of SiO 2 ACAs while providing insight to understand the origin of structural color of SiO 2 ACAs. [Display omitted] [ABSTRACT FROM AUTHOR]

Amorphous assembling of SiO 2 colloidal spheres is a convenient strategy to obtain amorphous photonic structure, such structure, referred to as SiO 2 amorphous colloidal arrays (ACAs), can demonstrate angle-independent structural color owing to constructive interference. To date, an empirical equation for materials researchers to design the color of SiO 2 ACAs remains vacant, and several influence factors concerning with the color of SiO 2 ACAs are still ignored and uninvestigated. Herein, based on single scattering model, we have proposed an empirical equation to predict the reflection peak of SiO 2 ACAs to design their color, and influence factors including background and thickness on the color of SiO 2 ACAs were also investigated. By preparing spheres within the designed range as building blocks, SiO 2 ACAs with colors varying from blue to red were achieved whose reflection peaks position fits well with the empirical equation. This study can help materials researchers to design and manipulate structural color of SiO 2 ACAs while providing insight to understand the origin of structural color of SiO 2 ACAs. [Display omitted] [ABSTRACT FROM AUTHOR]

In order to resolve the long-standing controversy surrounding the relationships within the Triplophysa genus, we conducted an extensive analysis of the complete mitogenome of Triplophysa labiata using DNBSEQ short reads. Additionally, we reconstructed the phylogeny of the Nemacheilidae family using mitogenome data. By comparing all available mitogenomes within the Triplophysa genus, we gained valuable insights into its evolutionary history. Our findings revealed that the mitogenome sequence of T. labiata is circular, spanning a length of 16,573 bp. It encompasses 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a control region (D-loop). Among the PCGs, the start codon ATG was commonly observed, except in cox1, while the stop codons TAA/TAG/T were found in all PCGs. Furthermore, purifying selection was evident across all PCGs. Utilizing maximum likelihood (ML) methods, we employed the 13 PCGs and the concatenated nucleotide sequences of 30 Triplophysa mitogenomes to infer the phylogeny. Our results strongly supported the division of the Triplophysa genus into four primary clades. Notably, our study provides the first evidence of the close relationship between T. labiata and T. dorsalis. These findings serve as a significant foundation for future investigations into the mitogenomics and phylogeny of Nemacheilidae fishes, paving the way for further advancements in this field of research. [ABSTRACT FROM AUTHOR]

Rewritable paper is of great significance for environmental and sustainable development. Specially, the water rewritable paper based on hydrochromism has aroused extensive research interesting for being clean, convenient and compatible with the current ink jet printer. Nevertheless, previously reported water rewritable paper requires complex and time-consuming fabricating process. Here, we report a facile strategy to fabricate structural colored water rewritable paper simply via two step infiltration on commercially available paper. After infiltration of graphene laminates and SiO 2 colloidal spheres in sequence, the obtained paper is integrated with assembled graphene laminates (AGLs)/amorphous colloidal arrays (ACAs) hierarchical composite structure. Owing to the advantageous micro-topography, selective separation capacity and high visible light absorption property of the underlying AGLs, SiO 2 ACAs with vivid structure color can be realized. Moreover, the hydrochomism of the hierarchical composite structure is resulted from the synergistic contribution of the hydrophilic structural color and the visible light absorption property of AGLs. Our strategy is based on the environmental-friendly structural color and convenient to realized, which offers a promising alternative to rapidly fabricate colored water rewritable paper. [Display omitted] [ABSTRACT FROM AUTHOR]

Background: The black-crowned night heron (Nycticorax nycticorax) is an ardeid bird successfully adapted to the nocturnal environment. Previous studies had indicated that the eyes of the night herons have evolved several specialized morphological traits favoring nocturnal vision. However, the molecular mechanisms of the nocturnal vision adaptation of night herons remained inattentions. In this study, the whole genome of N. nycticorax was sequenced and comparative analyses were performed on the vision-related and olfactory receptor (OR) genes to understand the molecular mechanisms of the visual and olfactory adaptation of night herons. Results: The results indicated that a number of vision genes were under positive or relaxed selection in N. nycticorax, whereas a number of other vision genes were under relaxed or intensified selection in the boat-billed heron (Cochlearius cochlearius), which suggested that the two species adapt to nocturnality with different genetic mechanisms. The different selections acting on vision genes are probably associated with the enlargement of eye size and the enhancement of visual sensitivity in night herons. The analyses on olfactory receptor (OR) genes indicated that the total number of OR genes in the genomes of N. nycticorax and C. cochlearius were about half those in the little egret (Egretta garzetta), whereas the diversity of their OR genes was not remarkably different. Additionally, the number of expressed OR genes in the transcriptomes of N. nycticorax was also fewer than that in E. garzetta. These results suggest a reduced olfactory capability in night herons compared with E. garzetta. Conclusions: Our results provided evidence that several vision genes of the night herons were subjected to different natural selections, which can contribute to a better understanding of the genetic mechanisms of visual adaptions of the night heron. In addition, the finding of the reduced number of total and expressed OR genes in night herons may reflect a trade-off between olfaction and vision. [ABSTRACT FROM AUTHOR]

Optical coherence tomography (OCT) is a noninvasive, radiation-free, and high-resolution imaging technology. The intraoperative classification of normal and cancerous tissue is critical for surgeons to guide surgical operations. Accurate classification of gastric cancerous OCT images is beneficial to improve the effect of surgical treatment based on the deep learning method. The OCT system was used to collect images of cancerous tissues removed from patients. An intelligent classification method of gastric cancerous tissues based on the residual network is proposed in this study and optimized with the ResNet18 model. Four residual blocks are used to reset the model structure of ResNet18 and reduce the number of network layers to identify cancerous tissues. The model performance of different residual networks is evaluated by accuracy, precision, recall, specificity, F1 value, ROC curve, and model parameters. The classification accuracies of the proposed method and ResNet18 both reach 99.90%. Also, the model parameters of the proposed method are 44% of ResNet18, which occupies fewer system resources and is more efficient. In this study, the proposed deep learning method was used to automatically recognize OCT images of gastric cancerous tissue. This artificial intelligence method could help promote the clinical application of gastric cancerous tissue classification in the future. [ABSTRACT FROM AUTHOR]

Enormous mountainous forests in Sino- Himalayans and Siberia harbor important avian biodiversity in the Northern Hemisphere. Numerous studies in last two decades have been contributed to systematics and taxonomy of passerines birds in these regions and have revealed various and complex phylogeographic patterns. A passerine species Red-flanked Bluetail Tarsiger cyanurus provided a good system to manifest such evolutionary complexity. The subspecies T. c. cyanurus and T. c. rufilatus (or/and T. c. pallidior), divergent in morphology, acoustics, and migratory strategies are allopatric in Siberia and Sino- Himalayan forests, respectively. The two taxa most likely deserve full species status but rigorous genetic analysis is missing. In this study, multilocus phylogeography based on mitochondrial DNA and Z-linked DNA reveals that T. c. cyanurus and T. c. rufilatus are reciprocally monophyletic with significant statistical support and differ with a large number of diagnostic nucleotide sites resulting substantial genetic divergence. Our finding supports the proposed split of Tarsiger cyanurus s.l. that T. cyanurus and T. rufilatus should be treated as two full species. Whether ' pallidior' is a subspecies or geographical form of T. rufilatus is still uncertain. Additionally, these two forest passerine species may have diverged 1.88 (3.25-1.30) Mya, which might be shaped by geographical vicariance due to grassland and desert steppe on the central Loess Plateau during the Pliocene. Taken together, this study and further suggests another independent example of North Palearctic-Sino-Himalayan phylogeographic pattern in Palearctic birds. [ABSTRACT FROM AUTHOR]

Controversies surrounding the phylogenetic relationships within the family Apogonidae have persisted due to the limited molecular data, obscuring the evolution of these diverse tropical marine fishes. This study presents the first complete mitochondrial genome of Fowleria variegata, a previously unrepresented genus, using high-throughput Illumina sequencing. Through a comparative mitogenomic analysis, F. variegate was shown to exhibit a typical genome architecture and composition, including 13 protein-coding, 22 tRNA and 2 rRNA genes and a control region, consistent with studies of other Apogonidae species. Nearly all protein-coding genes started with ATG, while stop codons TAA/TAG/T were observed, along with evidence of strong functional constraints imposed via purifying selection. Phylogenetic reconstruction based on maximum likelihood and Bayesian approaches provided robust evidence that F. variegata forms a basal lineage closely related to P. trimaculatus within Apogonidae, offering novel perspectives into the molecular evolution of this family. By generating new mitogenomic resources and evolutionary insights, this study makes important headway in elucidating the phylogenetic relationships and mitogenomic characteristics of Apogonidae fishes. The findings provide critical groundwork for future investigations into the drivers of diversification, speciation patterns, and adaptive radiation underlying the extensive ecological diversity and biological success of these marine fishes using phylogenomics and population genomics approaches. [ABSTRACT FROM AUTHOR]

In the last decade, the phylogenetic relationships within the genus Triplophysa have become controversial, due to a lack of molecular data. The mitochondrial genome plays a vital role in the reconstruction of phylogenetic relationships and in revealing the molecular evolution of bony fishes. Herein, we obtained the complete mitogenome of Triplophysa bombifrons via HiFi reads of the Pacbio Sequel II system and DNBSEQ short-reads. We compared all available mitogenomes of the Triplophysa genus and reconstructed the phylogeny of Nemacheilidae, based on the mitogenomes, using maximum likelihood (ML) methods. The results show that the complete mitogenome sequence of T. bombifrons was circular and 16,568 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA), 2 ribosomal RNA (rRNA), and a typical control region (D-loop). The most common start codons were ATG, except for cox1, and TAA/TAG were the stop codons for all PCGs. In total, 677 SNPs and 9 INDELs have been found by comparing the sequence divergence between this study and previous reports. Purity selection was found in all PCGs. Phylogeny was inferred by analyzing the 13 PCGs and the concatenated nucleotide sequences of 30 mitogenomes. The phylogenetic analyses based on the nucleotides of the 13 PCGs supported the assumption that the Triplophysa genus can be divided into 4 main clades and demonstrated that T. bombifrons and T. tenuis are closely related species for the first time. This study laid the foundation for further study on the mitogenome and phylogeny of Nemacheilidae fishes. [ABSTRACT FROM AUTHOR]

Double-roll rotary forging is an advanced partial progressive forming technology with multi-factor coupling interactive effects. To date, a few relevant researches on the double-roll rotary forging technology of large-diameter thin-walled discs have been reported, and the stable rolling conditions, stress field, velocity field, and so on are not studied in detail. Herein, a reasonable 3D rigid-plastic finite model of the double-roll hot rotary forging of a disk workpiece is established under the DEFORM software environment. Based on the valid 3D finite model, the distribution of stress field and velocity field and the influence of die preheating and initial size of workpiece on the uniformity and maximum axial load were studied. The research results can promote the application and development of rotary forging technology. [ABSTRACT FROM AUTHOR]

Double-roll rotary forging is an advanced partial progressive forming technology with multi-factor coupling interactive effects. To date, a few relevant researches on the double-roll rotary forging technology of large-diameter thin-walled discs have been reported, and the stable rolling conditions, stress field, velocity field, and so on are not studied in detail. Herein, a reasonable 3D rigid-plastic finite model of the double-roll hot rotary forging of a disk workpiece is established under the DEFORM software environment. Based on the valid 3D finite model, the distribution of stress field and velocity field and the influence of die preheating and initial size of workpiece on the uniformity and maximum axial load were studied. The research results can promote the application and development of rotary forging technology. [ABSTRACT FROM AUTHOR]

Simple Summary: We report the genomic sequence of Emberiza elegans for understanding the evolutionary mechanisms of environmental adaptation and for studying a more effective genetic monitoring of this species. The E. elegans assembly was approximately 1.14 Gb, with a scaffold N50 of 28.94 Mb. About 15,868 protein-coding genes were predicted, and 16.62% of the genome was identified as having repetitive elements. Our genomic evolution analyses found considerable numbers of adaptive genes that may help the yellow-throated bunting cope with migratory behavior and environmental stressors of diseases. These results provide us with new insights into genomic evolution and adaptation, thus providing a valuable resource for further studies of population genetic diversity and genome evolution in this species. Yellow-throated bunting is a small migratory songbird unique to the Palearctic region. However, the genetic studies of this species remain limited, with no nuclear genomic sequence reported to date. In this study, the genomic DNA from the bird was sequenced in long reads using Nanopore sequencing technology. Combining short-read sequencing, the genome was well-assembled and annotated. The final length of the assembly is approximately 1.14 Gb, with a scaffold N50 of 28.94 Mb. About 15,868 protein-coding genes were predicted, and 16.62% of the genome was identified as having repetitive elements. Comparative genomic analysis showed numerous expanded gene families and positively selected genes significantly enriched in those KEGG pathways that are associated with migratory behavior adaptation and immune response. Here, this newly generated de novo genome of the yellow-throated bunting using long reads provide the research community with a valuable resource for further studies of population genetic diversity and genome evolution in this species. [ABSTRACT FROM AUTHOR]

Double-roll rotary forging is an emerging plastic forming technology based on rotary forging. Owing to the advantages of labor saving, small eccentric load, low noise and vibration, good uniformity, high surface quality, and material saving, it is very promising for fabrication of large-diameter thin-walled disc. To date, a few relevant researches on the double-roll rotary forging technology of large-diameter thin-walled metal discs have been reported, and the forming mechanisms and process of disc workpieces remain uninvestigated. Herein, a 3D rigid-plastic finite element model (FEM) is established to simulate the fabrication process of large-diameter thin-walled disc; four geometric features appear in the forming process: "mushroom" shape, "upper drum" shape, "drum shape" shape, and "lower drum" shape. Equivalent stress, equivalent strain, and temperature field of these four geometric shapes are analyzed, and the forming mechanism of these four geometric shapes is revealed. The reliability and accuracy of FEM are verified through experiments, and the four geometric shape features occur in the process are consistent with the simulation. The research results provide valuable guidelines for better understanding of double-roll rotary forging for the fabrication of large-diameter thin-walled discs. [ABSTRACT FROM AUTHOR]

Forming tight interaction with both Purkinje and granule cells (GCs), Bergmann glia (BG) are essential for cerebellar morphogenesis and neuronal homeostasis. However, how BG act in this process is unclear without comprehensive transcriptome landscape of BG. Here, high temporal‐resolution investigation of transcriptomes with FACS‐sorted BG revealed the dynamic expression of genes within given functions and pathways enabled BG to assist neural migration and construct neuron‐glia network. It is found that the peak time of GCs migration (P7‐10) strikingly coincides with the downregulation of extracellular matrix (ECM) related genes, and the disruption of which by Setdb1 ablation at P7‐10 in BG leads to significant migration defect of GCs emphasizing the criticality of Nfix‐Setdb1 mediated H3K9me3 repressive complex for the precise regulation of GCs migration in vivo. Thus, BG's transcriptomic landscapes offer an insight into the mechanism by which BG are in depth integrated in cerebellar neural network. [ABSTRACT FROM AUTHOR]

Forming tight interaction with both Purkinje and granule cells (GCs), Bergmann glia (BG) are essential for cerebellar morphogenesis and neuronal homeostasis. However, how BG act in this process is unclear without comprehensive transcriptome landscape of BG. Here, high temporal‐resolution investigation of transcriptomes with FACS‐sorted BG revealed the dynamic expression of genes within given functions and pathways enabled BG to assist neural migration and construct neuron‐glia network. It is found that the peak time of GCs migration (P7‐10) strikingly coincides with the downregulation of extracellular matrix (ECM) related genes, and the disruption of which by Setdb1 ablation at P7‐10 in BG leads to significant migration defect of GCs emphasizing the criticality of Nfix‐Setdb1 mediated H3K9me3 repressive complex for the precise regulation of GCs migration in vivo. Thus, BG's transcriptomic landscapes offer an insight into the mechanism by which BG are in depth integrated in cerebellar neural network. [ABSTRACT FROM AUTHOR]

Abstract: Purpose: The objective of this study was to assess the spontaneous oscillations in subjects with hypertension based on the wavelet transform of cerebral oxygenation signal measured with near-infrared spectroscopy (NIRS). Methods: Continuous recordings of NIRS and arterial blood pressure (ABP) signals were obtained from simultaneous measurements in 20 healthy subjects (age: 70.8±5.2years) and 22 subjects with hypertension (age: 72.5±6.8years). Results: Using spectral analysis based on wavelet transform, five frequency intervals were identified (I, 0.4–2Hz; II, 0.15–0.4Hz; III, 0.06–0.15Hz; IV, 0.02–0.06Hz and V, 0.005–0.02Hz). The amplitudes of Δ[Hb] and Δ[HbO2] in intervals I, II and III were significantly higher in hypertensive patients, who have increased mean flow velocity in middle cerebral artery (MCA), compared to that in the healthy subjects (p<0.01). The amplitudes of the ABP in frequency intervals III and V were significantly higher in hypertensive patients than in the healthy subjects (p<0.01). Conclusions: The present findings revealed that hypertension and increased mean flow velocity in MCA have significant effect on the cerebral oscillations. The higher cerebral oscillations might be related to the intracerebral atherosclerosis in response to systemic hypertension. In addition, the higher spontaneous oscillations in intervals III and V in ABP indicate a metabolic regulation and myogenic response to hypertension. [Copyright &y& Elsevier]

The study aims to assess the spontaneous oscillations in elderly subjects based on the wavelet transform of cerebral oxygenation (CO) and arterial blood pressure (ABP) signals. Continuous recordings of near-infrared spectroscopy (NIRS) and ABP signals were obtained from simultaneous measurements in 20 young subjects (age: 27.3±7.1 years) and 15 elderly subjects (age: 70.8±5.1 years) at rest. Using spectral analysis based on wavelet transform, five frequency intervals were identified (I, 0.005 to 0.02 Hz; II, 0.02 to 0.06 Hz; III, 0.06 to 0.15 Hz; IV, 0.15 to 0.40 Hz; and V, 0.40 to 2.0 Hz). The average amplitudes of the Δ[HbO2] and tissue oxygenation index in intervals I to V and the relative amplitudes in intervals IV and V were significantly lower in elderly subjects than in young subjects (P<0.05). In addition, the relative amplitudes of the ABP in interval I were significantly lower in elderly subjects than in young subjects (P=0.016). The present findings suggest the presence of a cerebrovascular degenerative process caused by aging. Spontaneous oscillations in the CO could be used as an indicator of cerebrovascular changes and could be used to identify the risk for cerebrovascular degenerative processes. [ABSTRACT FROM AUTHOR]

Abstract: Geographic variation in the Black-throated Laughingthrush (Garrulax chinensis) is examined to infer the influence of Pleistocene glacial oscillations on the genetic diversity of its subspecies. Mitochondrial evidence suggests that the endemic Hainan Island taxon, G. c. monachus, is monophyletic, whereas its closest continental relatives, G. c. chinensis and G. c. lochmius, are not. Multilocus coalescent analysis based on two mitochondrial and two nuclear intron loci indicates inter-subspecific genetic differentiation during the Pleistocene and substantial post-divergence genetic introgression from G. c. chinensis into G. c. lochmius. In contrast, G. c. monachus experienced no post-divergence gene flow despite occasional land-bridge contact with its continental relatives, suggesting its isolation may have been imposed by ecology as well as geography. It is probably reproductively isolated and should be treated as a distinct species. [Copyright &y& Elsevier]

The objective of this study was to assess the effects of whole-body vibration (WBV) on lumbar muscle oxygenation oscillations in healthy men based on the wavelet transform of near-infrared spectroscopy signals. Twelve healthy participants were exposed to WBV at frequencies of 3, 4.5 and 6 Hz while muscle oxygenation signal was monitored before, during and recovery from WBV. With spectral analysis based on wavelet transform of NIR signal, six frequency intervals were identified (I, 0.005-0.0095 Hz; II, 0.0095-0.02 Hz; III, 0.02-0.06 Hz; IV, 0.06-0.16 Hz; V, 0.16-0.40 Hz and VI, 0.40-2.0 Hz). It was found that the muscle oxygenation oscillations at 4.5 Hz in the frequency intervals I, II and III was lower during WBV compared with that of at 3 Hz. Present results demonstrated WBV at 4.5 Hz induced lower oscillatory activities than that of at 3 Hz. The lower oscillatory activities might indicate a decrease in the efficiency of oxygen supply to the oxygenated tissue and such mechanism might contribute to the development of local muscle fatigue. [ABSTRACT FROM AUTHOR]