50 results on '"Lupulović, Diana"'
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2. Effect of Different Temperatures on the Microbiological Status of Donkey Milk
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Knežević Suzana Vidaković, Vranešević Jelena, Lupulović Diana, Kartalović Brankica, Knežević Slobodan, and Pajić Marija
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donkey milk ,hand milking ,microorganisms ,Agriculture - Abstract
Donkey milk is usually sold for human consumption directly at farms and agricultural households and it is used in the raw state because of its well-known medicinal properties. Sometimes, however, such milk has poor microbiological status due to inappropriate conditions during hand milking and storage. The aim of this paper was to examine the microbiological status of donkey milk exposed to different temperature treatments (refrigerated at 4 ℃ for 3 h, frozen at −18 ℃ for 1 day, and pasteurized at 80 ℃ for 10 minutes). The number of molds and yeasts, total viable count, Enterobacteriaceae, Escherichia coli, coagulase-positive Staphylococcus, and the presence of foodborne pathogens, including Salmonella spp., Listeria monocytogenes, and Campylobacter spp. were evaluated to estimate the microbiological status of donkey milk. As expected, freezing and pasteurization reduced the number of tested microorganisms, and no examined foodborne pathogens were detected. The total viable count was reduced by 0.64 log CFU/mL by freezing and by 2.23 log CFU/mL by pasteurization. The total molds and yeasts and total Enterobacteriaceae count were significantly reduced by pasteurization. In conclusion, frozen and pasteurized donkey milk is safer than raw milk. In order to be able to recommend the best temperature treatment before consumption, the effects of freezing and pasteurization on the quality and antibacterial properties of donkey milk have to be further investigated.
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- 2023
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3. Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods
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Samojlović Milena, Polaček Vladimir, Gurjanov Vladimir, Lupulović Diana, Lazić Gospava, Petrović Tamaš, and Lazić Sava
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antibodies ,elisa ,lumpy skin disease virus ,virus neutralization test ,specificity ,sensitivity ,Veterinary medicine ,SF600-1100 - Abstract
Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow to improve serological diagnostics of lumpy skin disease (LSD).
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- 2019
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4. Evidence of the first clinical case of equine neuroinvasive West Nile disease in Serbia, 2018
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Medić Strahinja, Lazić Sava, Petrović Tamaš, Petrić Dušan, Samojlović Milena, Lazić Gospava, and Lupulović Diana
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west nile virus ,neuroinvasive disease ,horses ,serbia ,Veterinary medicine ,SF600-1100 - Abstract
During July 2018, the first clinical case of neurological West Nile virus (WNV) infection was reported in a Belgian sports mare in Belgrade, Serbia. Typical symptoms, such as hypersensitive skin reaction, disorientation, weakness, ataxia and the loss of equilibrium were reported. Detection of WNV IgM antibodies by commercial ELISA in the serum samples of the diseased mare strongly indicated acute infection. The ELISA positive results were confirmed by VNT. Hematological and biochemical parameters were in the reference range. The only finding was a minor lymphopenia. WNV RNA was not detected by RT-qPCR in the blood sample extracted seven days after the disease had broken out. The horse improved clinically in two weeks while other horses at the same premises remained asymptomatic. The clinical, serological, biochemical and molecular analyses applied confirmed the first clinical case of neuroinvasive WNV infection in horses in Serbia. The West Nile virus has been circulating in Serbia in the last decade in mosquitoes, birds, and horses, but no evidence of equine WNV clinical cases were registered so far.
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- 2019
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5. Complete genome sequences of two strains of classical swine fever virus of subgenotype 2.3 detected during outbreaks in 2005 and 2006 in Serbia
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Petrović, Tamaš, Kuhar, Urška, Lazić, Sava, Prodanov-Radulović, Jasna, Lupulović, Diana, Lazić, Gospava, and Toplak, Ivan
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- 2019
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6. First Detection and Clinical Presentation of Porcine Epidemic Diarrhea Virus (Pedv) in Serbia
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Prodanov-Radulović Jasna, Petrović Tamaš, Lupulović Diana, Marčić Doroteja, Petrović Jelena, Grgić Živoslav, and Lazić Sava
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porcine epidemic diarrhea virus (pedv) ,swine farm ,serbia ,Veterinary medicine ,SF600-1100 - Abstract
During 2015/2016, fecal and intestinal samples from live diseased and/or dead pigs with suspected PEDV and/or TGEV and signs of rotavirus infection were collected from in total seven different farrow-to-finish swine farms located in Northern Serbia region (Vojvodina Province). A total of 14 samples (2 pools per farm) of small intestine with fecal content were submitted to laboratory molecular investigation (multiplex RTPCR). On these farms the clinical signs included the occurrence of diarrhea in suckling and weaned piglets, with weak or no response to the applied antimicrobial therapy. The epidemic of severe diarrhea affecting pigs of all ages on one farrow-to finish swine farm was detected in January 2016. Watery diarrhea in all swine categories was associated with vomiting and a reduction in feed consumption. Diarrheic, gaunt and dehydrated piglets, covered with feces were found in 90% litters. The disease affected most severely the suckling piglets, and the mortality in newborn piglets was up to 35%. In the weaned piglets and fatteners the mortality was up to 2.5% and 1.2%, respectively. The PEDV RNA was detected in pooled feces and samples of small intestines derived from diseased and dead suckling piglets from only one investigated farm. The PEDV positive samples showed to be negative for rotavirus group A and TGEV. The transport vehicles were identified as the main possible route of PEDV introduction. This is the first report demonstrating the presence of PEDV in Serbia.
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- 2017
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7. Seroprevalence of West Nile virus in domestic donkeys in the Special Nature Reserve 'Zasavica', Serbia
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Lupulović, Diana, Tamaš Petrović, Lazić, Gospava, Gajdov, Vladimir, Bugarski, Dejan, and Lazić, Sava
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- 2023
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8. Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve "Zasavica", Serbia.
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Lazić, Sava, Savić, Sara, Petrović, Tamaš, Lazić, Gospava, Žekić, Marina, Drobnjak, Darko, and Lupulović, Diana
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EQUINE infectious anemia ,NATURE reserves ,DONKEYS ,VIRUS diseases ,PLANT viruses ,EQUINE influenza ,ENZYME-linked immunosorbent assay ,ANTIBODY titer - Abstract
Simple Summary: The domestic donkey is an endangered equid species. There are 199 donkeys in the Special Nature Reserve "Zasavica", out of the estimated 1000 that are assumed to exist in Serbia. The aim of this study was to assess the exposure of donkeys in the Special Nature Reserve "Zasavica" to some of the most significant viral pathogens and establish the seroprevalence of equine infectious anemia, African horse sickness, equine herpesvirus type 1 infection, equine influenza and equine viral arteritis. In tested blood samples, antibodies against equine herpesvirus type 1 and equine influenza virus (subtype H3N8) were found, while all samples tested negative for African horse sickness virus, equine infectious anemia virus and equine arteritis virus. To our knowledge, this study represents the first results of detection of these pathogens in the population of domestic donkeys in Serbia. The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in "Zasavica", blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test). [ABSTRACT FROM AUTHOR]
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- 2023
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9. The program for eradication of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis in the Republic of Serbia
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Lazić Sava, Petrović Tamaš, Bugarski Dejan, Jovičin Milovan, Plavšić Budimir, Lupulović Diana, Lazić Gospava, and Polaček Vladimir
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IBR/IPV ,the Republic of Serbia ,program ,eradication ,implementation ,Veterinary medicine ,SF600-1100 - Abstract
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) are diseases that affect cattle population of all breeds, categories and age. Both diseases, especially infectious bovine rhinotracheitis (IBR), poses severe health threat and causes major economic losses and is considered one of the “most costly” disease in cattle industry. The causal agent of the disease is a virus and any detection of IBR/IPV specific antibodies in non-vaccinated cattle, either in blood or milk, indicates that animal is infected and represents the source of infection. Countries with developed and intensive cattle breeding have been developed and implemented their national eradication programs to control IBR/IPV in accordance with international regulations. In this article, we outlined the needs and program for the eradication of IBR/IPV in the Republic of Serbia. The eradication program for IBR/IPV is an extensive process that requires systematic strategy involving different phases and activities. The eradication process from the moment of implementation until obtaining IBR/IPV-free status can last over several years and requires joint work and considerable financial resources that will be compansated with the elimination of IBR/IPV from the herd. This article gives an overview of all stages and activities regarding eradication of the disease and certification and maintaining of IBR/IPV-free herd status.
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- 2016
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10. Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
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Lazić Sava, Lupulović Diana, Polaček Vladimir, Valčić Miroslav, Lazić Gospava, Pašagić Enisa, and Petrović Tamaš
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breeding stallions ,semen ,equine arteritis virus ,rt-pcr ,seroprevalence ,virus neutralization ,Veterinary medicine ,SF600-1100 - Abstract
The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
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- 2015
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11. WNV infection - an emergent vector borne viral infection in Serbia: Current situation
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Petrović Tamaš, Lupulović Diana, Petrić Dušan, Vasić Ana, Hrnjaković-Cvjetković Ivana, Milošević Vesna, Vidanović Dejan, Šekler Milanko, Lazić Sava, Đuričić Bosiljka, Plavšić Budimir, and Saiz Juan-Carlos
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West Nile virus ,antibody and virus detection ,domestic and wild animals ,human population ,mosquitoes ,surveillance program ,Serbia ,Veterinary medicine ,SF600-1100 - Abstract
West Nile virus (WNV) is a neurovirulent mosquito-borne Flavivirus with zoonotic potential. Virus is maintained in nature in an enzootic transmission cycle between avian hosts and mosquito vectors, but occasionally infects other vertebrates. The infection in horses and humans can be asymptomatic or it can have different clinical manifestations ranging from light febrile diseases to fatal meningoencephalitis. Recently, the number, frequency and severity of outbreaks with neurological consequences for birds, humans and horses have increased dramatically throughout central and south Europe, including Serbia, posing a serious veterinary and public health problem. The emergency of WNV infections in Serbia is described through the current epidemiology situation based on recent data on the incidence of WNV infection among virus natural hosts and vectors; sentinel (horses) and other animal species, and in human population. The results of the WNV serology studies conducted on horse blood samples collected in different occasions during the last six years, and the results of the serology studies conducted among other animal species like pigs, wild boars, roe deer and dogs in Serbia are presented and discussed. Also, the results of the first studies on WNV presence in mosquito vectors and in wild birds as virus natural hosts in Serbia are presented and analyzed. In addition, the data on the WNV serology studies conducted in human population in Serbia in the last few years, and the existing data of WNV outbreaks in 2012 and 2013 are included. Regarding the existing knowledge on WNV epidemiology situation, the crucial role of veterinary service in early detection of WNV presence and ongoing national program of WNV surveillance in sentinel animals, mosquitoes and wild birds are discussed.
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- 2015
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12. The establishment of the cell culture of preadipocytes of common carp (Cyprinus carpio L.)
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Ljubojević Dragana B., Pelić Miloš M., Novakov Nikolina J., Lazić Sava M., Lupulović Diana M., Ćirković Miroslav A., and Todorčević Marijana Lj.
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common carp ,preadipocytes ,isolation ,cell culture ,fat tissue ,Food processing and manufacture ,TP368-456 - Abstract
Common carp is the most important freshwater fish in aquaculture in Republic of Serbia. Excess fat deposition in the meat and abdominal cavity of farmed carp can affect carp flesh quality, dressing percentage and consequently restrict the further development of aquaculture production. The reasons for lipid deposition in carp flesh and around visceral organs are not well known, and it is important to develop method which would enable us to understand process which occurs in common carp lipid cells. The aim of this study was to establish a new model of common carp preadipocytes and evaluate proliferation and differentiation capacity of carp preadipocytes in vitro. The establishment of the cell culture of preadipocytes of common carp could serve as a valuable tool for studying fat metabolism in this fish species.
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- 2014
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13. Serological study on WNV presence in horses in Vojvodina after the human outbreak in Serbia in 2012
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Petrović T., Lazić S., Lupulović Diana, Lazić Gospava, Bugarski D., Vidanović D., Stefan-Mikić Sandra, Milošević Vesna, Hrnjaković-Cvetković Ivana, and Petrić D.
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WNV ,horses ,serology ,mosquitoes ,RT-PCR ,Serbia ,Biology (General) ,QH301-705.5 - Abstract
To establish the presence of West Nile virus (WNV) infection in the animal population in Serbia after the human WNV outbreak, the presence of anti-WNV IgG antibodies was examined by commercial ELISA of blood sera samples of 130 horses collected in 2012 from 6 stables and 1 settlement in Vojvodina Province, northern Serbia. During the blood sampling, hibernating mosquitoes in the vicinity of the sampled horses were collected (31 pools from 4 locations) and tested for WNV presence by real-time RT-PCR. The presence of anti-WNV antibodies was observed in 49.23% (64/130) horses. Per stable, the percent of seropositive animals ranged from 35% to 64%. All 31 analyzed pools of hibernating mosquitoes tested negative for WNV RNA. The WNV-antibody prevalence of 49.23% obtained in horses during 2012 was much higher than the prevalence (12%) found in horses during 2009/2010. These results, including the confirmed seroconversion in eight horses that tested negative in 2010, indicated an intensive WNV circulation during 2012 in Serbia, and the necessity of implementing surveillance programs. [Projekat Ministarstva nauke Republike Srbije, br. TR31084 and III43007]
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- 2014
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14. Presence of Human and Animal Viruses in Surface Waters in Vojvodina Province of Serbia
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Lazić, Gospava, Grubač, Siniša, Lupulović, Diana, Bugarski, Dejan, Lazić, Sava, Knežević, Petar, and Petrović, Tamaš
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- 2015
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15. Investigations of presence of antibodies against bovine herpesvirus-1 in blood serum of calves prior to colostrum diet
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Lazić Sava, Rogan Dragan, Petrović Tamaš, Bugarski Dejan, Lupulović Diana, and Lazarević Miodrag
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antibodies ,BHV-1 ,cows ,valves ,precolostral blood serum ,Veterinary medicine ,SF600-1100 - Abstract
The paper presents the results of investigations of the presence of the bovine herpesvirus-1 (BHV-1) in samples of blood serum from 106 cows and 107 of their calves (one cow had twins). Blood was sampled from the cows immediately after parturition, and from the calves before feeding on colostrum. The examined cows and their calves originated from 5 herds in which previous investigations had shown infection with the bovine herpesvirus-1. The determination of antibodies against BHV-1 was performed using the method of virus neutralization in culture of MDBK cells with 100 TCID/50 viruses (BHV-1, TN-41 Am. Bio Research, USA). Antibodies against BHV-1 were determined in all blood serum samples of cows and in 16 samples of precolostral blood serums of calves. The antibody titer values in cows ranged from 1:4 to 1:512, and in calves the determined values were from 1:2 to 1:16. The results indicate that cows that are seropositive to BHV-1 can deliver calves seropositive to BHV-1 in about 15% cases. This must be kept in mind in selecting cows for the production of breeding material, in particular bulls for reproduction centers, as well as in making a programme for the immunoprophylaxis of calves against BHV-1. .
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- 2010
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16. HONEYBEE VIRUSES PRESENCE IN SERBIAN APIARIES: A REVIEW
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Petrovic, Tamas, primary, Vidanović, Dejan, additional, Lupulović, Diana, additional, Lazić, Gospava, additional, and Lazić, Sava, additional
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- 2021
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17. Intensive West Nile Virus Circulation in Serbia in 2018—Results of Integrated Surveillance Program
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Petrović, Tamaš, primary, Šekler, Milanko, additional, Petrić, Dušan, additional, Vidanović, Dejan, additional, Debeljak, Zoran, additional, Lazić, Gospava, additional, Lupulović, Diana, additional, Kavran, Mihaela, additional, Samojlović, Milena, additional, Ignjatović Ćupina, Aleksandra Ignjatović, additional, Tešović, Bojana, additional, Lazić, Sava, additional, Kolarević, Mišo, additional, Labus, Tatjana, additional, and Djurić, Boban, additional
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- 2021
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18. Investigations of significance of vaccination against swine parvovirosis in persistently infected sows
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Lupulović Diana, Milić Nenad, Petrović Tamaš, Prodanov Jasna, and Lazić Sava
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swine parvovirosis ,persistent infection ,inactivated vaccines ,Veterinary medicine ,SF600-1100 - Abstract
Parvoviral infection of swine is a disease which is manifested in reproductive disorders in sows and gilts in the form of anestria, premature births, miscarriages, mummified fetuses, the birth of poorly vital piglets and/or a reduced number of piglets in the litter. The infection is present in farms with intensive breeding conditions in the form of an endemic infection, all over the world, and also in our country. Timely diagnostics and adequate prophylaxis prevent the occurrence and spread of this disease. Experimental investigations covered 21 sows, divided into two experimental and a third, the control, group. Animals of the first experimental group were vaccinated once before exposure to the boar using an inactivated vaccine, Porcilis Parvo, and animals of the second experimental group were vaccinated twice at an interval of 3 weeks, also using an inactivated vaccine, Parvovax. Sows of the control group were not vaccinated. Blood samples were taken from all animals four times during the course of the experiment, and specific antibodies against the swine parvovirus were determined using the method of hemagglutination inhibition (HI test). The results of the investigations indicate that there was an increase in the titre of specific antibodies following the vaccination of persistently infected sows with the swine parvivirus, and that the present antibodies did not prevent the creating of an immune response. It was established following a comparison of the geometric mean values of antibody titres of vaccinated sows that there was a significant increase in the antibody level following the two vaccinations using the Parvovax vaccine, against the titre values in sows vaccinated once with the Porcilis Parvo vaccine. In control animals, the average value of the antibody level was many times lower in comparison with the established values in the experimental groups. This provides justification for the implementation of immunoprophylaxis against swine parvovirosis by the vaccination of sows and gilts before mating using inactivated vaccines.
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- 2007
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19. Significance of determining intrauterine infections with classical swine plague virus within programme of curbing and eradicating this disease
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Prodanov Jasna, Došen Radoslav, Petrović Tamaš, Lupulović Diana, Valčić Miroslav, and Polaček Vladimir
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classical swine plague ,intrauterine infection ,eradication ,Veterinary medicine ,SF600-1100 - Abstract
Intrauterine infection of the fetus occurs if a pregnant non-vaccinated sow is infected with the virus of classical swine plague (CSF). The infection of the fetus results in the occurrence of viremia and the distribution of the virus in fetal tissue is similar to the distribution which is established in post-natal infected swine. The objective of these investigations was to determine intrauterine (transplacental) infection in the event of the appearance of CSF in different periods of pregnancy in non-vaccinated and vaccinated sows. The examined material were organs and tissue of fetuses within two examined cases of CSF in non-immune pregnant sows. In the third examined case of CSF, the material comprised the blood of piglets before suckling the colostrum, animals originating from vaccinated sows, at a farm in which CSF had been diagnosed. Samples of tissue and blood of the piglets were examined for the presence of antigens and specific antibodies against the CSF virus using the immunoenzyme technique (ELISA). Even though the investigations were performed on a small number of samples, the obtained results raise the question of the possibility of the occurrence of intrauterine infection with a CSF field virus in sows vaccinated with the C-strain of CSF. The syndrome of a carrier sow and persistent infections are the chief problem factors that need to be considered within the programme of curbing and eradicating classical swine plague.
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- 2007
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20. New knowledge in application of vaccines with Kina(K)strain in control and curbing of classical swine fever
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Prodanov Jasna, Došen Radoslav, Polaček Vladimir, Petrović Tamaš, and Lupulović Diana
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classical swine fever ,vaccination ,Kina strain ,Veterinary medicine ,SF600-1100 - Abstract
The program of curbing and outrooting classical swine fever (CSF) in our country has for years been based on the vaccination of all categories of swine with the Kina (K) strain of the CSF virus, and, after the establishment of the source of infection, with the non-harmful removal of the infected swine herd, as well as on the implementation of all measures prescribed by the valid regulations. However, in spite of the implementation of all the listed measures, CSF still occurs continually. The epizootiological situation in our country in the course of 2005 was extremely unfavorable from the aspect of the number of identified CSF crisis spots, and it can be pointed out at this time that the disease was diagnosed clinically, pathomorphologically and using laboratory methods in 51 farms in a single examined epizootiological district. Under conditions when CSF appears continually throughout the year, vaccination using a modified live vaccine must continue being the basic measure in preventing the appearance, spreading, curbing, and eradication of CSF. With the objective of securing a concept of the most efficient further strategy for control and curbing of this disease, the paper presents the most important aspects regarding efficacy, safety, as well as field experience in applying vaccines which are based on the K strain of the CSF virus. .
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- 2006
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21. Immunoenzyme: Elisa diagnostics in veterinary medicine
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Đurišić Slavko, Lazić Sava, Petrović Tamaš, Savić-Jevđenić Sara, and Lupulović Diana
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immunoenzyme - ELISA technique ,laboratory diagnostics ,veterinary medicine ,Veterinary medicine ,SF600-1100 - Abstract
The ELISA technique is one of the most commonly used laboratory-diagnostical methods for diagnosing many infective diseases in humans and animals today. Due to its high specificity and sensitivity this technique is considered a method of choice in diagnosing many viral diseases. The phases and components of the ELISA technique are constantly improved because of its mass use, as well as the high demands of laboratory diagnostics. Therefore it is necessary to continually inform the expert public of all the innovations, forms and possibilities of the ELISA technique. This paper describes the factors that caused the genesis of the ELISA technique and shows its forms. The methodological and practical aspects of the ELISA technique, when applied in laboratory diagnostics of veterinary medicine are also presented.
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- 2003
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22. Importance of Multidisciplinary and Regional Collaboration in Integrated West Nile Virus Surveillance - the „One Health” Concept
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Vilibić-Čavlek, Tatjana, primary, Vidanović, Dejan, additional, Barbić, Ljubo, additional, Jeličić, Pavle, additional, Lazić, Sava, additional, Radmanić, Luka, additional, Lupulović, Diana, additional, Janev-Holcer, Nataša, additional, Tešović, Bojana, additional, Milošević, Vesna, additional, Stevanović, Vladimir, additional, Cvjetković-Hrnjaković, Ivana, additional, Lazić, Gospava, additional, Bogdanić, Maja, additional, Tabain, Irena, additional, Klobučar, Ana, additional, Šekler, Milanko, additional, Savić, Vladimir, additional, Petrović, Tamaš, additional, and Petrić, Dušan, additional
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- 2020
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23. EVIDENCE OF THE FIRST CLINICAL CASE OF EQUINE NEUROINVASIVE WEST NILE DISEASE IN SERBIA, 2018 'Vetlab' doo, Veterinary laboratory for clinical pathology
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Medić Strahinja, Lazić Sava, Petrović Tamaš, Petrić Dušan, Samojlović Milena, Lazić Gospava, and Lupulović Diana
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- 2019
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24. AUJESZKY’S DISEASE IN A DOG - CASE REPORT
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Lazić, Gospava, primary, Petrović, Tamaš, additional, Lupulović, Diana, additional, Topalski, Bojan, additional, Božić, Biljana, additional, and Lazić, Sava, additional
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- 2019
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25. Complete Genome Sequence of an Equine Arteritis Virus Strain Isolated from a Lipizzaner Stallion in 2015 in Serbia
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Gaudaire, Delphine, primary, Lazić, Sava, additional, Lupulović, Diana, additional, Petrovic, Tamas, additional, Lazić, Gospava, additional, Berthet, Nicolas, additional, and Hans, Aymeric, additional
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- 2019
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26. Flaviviruses at the territory of Serbia – present situation and challenges
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Petrović, Tamaš, primary, Šekler, Milanko, additional, Petrić, Dušan, additional, Vidanović, Dejan, additional, Potkonjak, Aleksandar, additional, Hrnjaković Cvjetković, Ivana, additional, Savić, Sara, additional, Debeljak, Zoran, additional, Lazić, Gospava, additional, Ignjatović Ćupina, Aleksandra, additional, Lupulović, Diana, additional, Samojlović, Milena, additional, Jurišić, Aleksandar, additional, Petrović, Aleksandra, additional, Ivanović, Ivana, additional, Milošević, Vesna, additional, and Lazić, Sava, additional
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- 2019
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27. Complete genome sequences of two strains of classical swine fever virus of subgenotype 2.3 detected during outbreaks in 2005 and 2006 in Serbia
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Petrović, Tamaš, primary, Kuhar, Urška, additional, Lazić, Sava, additional, Prodanov-Radulović, Jasna, additional, Lupulović, Diana, additional, Lazić, Gospava, additional, and Toplak, Ivan, additional
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- 2018
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28. Methodology and results of integrated WNV surveillance programmes in Serbia
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Petrović, Tamaš, primary, Šekler, Milanko, additional, Petrić, Dušan, additional, Lazić, Sava, additional, Debeljak, Zoran, additional, Vidanović, Dejan, additional, Ignjatović Ćupina, Aleksandra, additional, Lazić, Gospava, additional, Lupulović, Diana, additional, Kolarević, Mišo, additional, and Plavšić, Budimir, additional
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- 2018
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29. Serological evidence of equine arteritis virus infection and phylogenetic analysis of viral isolates in semen of stallions from Serbia
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Lazić, Sava, primary, Lupulović, Diana, additional, Gaudaire, Delphine, additional, Petrovic, Tamas, additional, Lazić, Gospava, additional, and Hans, Aymeric, additional
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- 2017
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30. West Nile virus serosurveillance in pigs, wild boars, and roe deer in Serbia
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Escribano-Romero, Estela, Lupulović, Diana, Merino-Ramos, Teresa, Blázquez, Ana-Belén, Lazić, Gospava, Lazić, Sava, Saiz, Juan-Carlos, and Petrović, Tamaš
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- 2015
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31. West Nile virus ‘circulation’ in Vojvodina, Serbia: Mosquito, bird, horse and human surveillance
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Petrić, Dušan, primary, Petrović, Tamaš, additional, Hrnjaković Cvjetković, Ivana, additional, Zgomba, Marija, additional, Milošević, Vesna, additional, Lazić, Gospava, additional, Ignjatović Ćupina, Aleksandra, additional, Lupulović, Diana, additional, Lazić, Sava, additional, Dondur, Dragan, additional, Vaselek, Slavica, additional, Živulj, Aleksandar, additional, Kisin, Bratislav, additional, Molnar, Tibor, additional, Janku, Djordje, additional, Pudar, Dubravka, additional, Radovanov, Jelena, additional, Kavran, Mihaela, additional, Kovačević, Gordana, additional, Plavšić, Budimir, additional, Jovanović Galović, Aleksandra, additional, Vidić, Milan, additional, Ilić, Svetlana, additional, and Petrić, Mina, additional
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- 2017
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32. PROSPECTIVES AND NECESSITY OF ERADICATION OF INFECTIOUS BOVINE RHINOTRACHEITIS / INFECTIOUS PUSTULAR VULVOVAGINITIS IN THE REPUBLIC OF SERBIA
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Lazić, Sava, primary, Petrović, Tamaš, additional, Bugarski, Dejan, additional, Jovičin, Milovan, additional, Plavšić, Budimir, additional, Lupulović, Diana, additional, Lazić, Gospava, additional, and Polaček, Vladimir, additional
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- 2016
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33. Importance of Multidisciplinary and Regional Collaboration in Integrated West Nile Virus Surveillance - the „One Health" Concept.
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Vilibić-Čavlek, Tatjana, Petrović, Tamaš, Savić, Vladimir, Šekler, Milanko, Klobučar, Ana, Petrić, Dušan, Tabain, Irena, Vidanović, Dejan, Bogdanić, Maja, Lazić, Gospava, Cvjetković-Hrnjaković, Ivana, Stevanović, Vladimir, Milošević, Vesna, Tešović, Bojana, Janev-Holcer, Nataša, Lupulović, Diana, Radmanić, Luka, Lazić, Sava, Jeličić, Pavle, and Barbić, Ljubo
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WEST Nile fever diagnosis ,ARBOVIRUSES ,EPIDEMIOLOGY - Abstract
Copyright of Croatian Journal of Infection / Infektoloski Glasnik is the property of Croatian Society for Infectious Diseases and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2019
- Full Text
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34. Razvoj i primena različitih laboratorijskih metoda za dijagnostikovanje infekcije izazvane hepatitis E virusom kod svinja i ljudi
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Lupulović, Diana, Lako, Branislav, Lazić, Sava, Knežević-Ušaj, Slavica, and Gagrčin, Mladen
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ljudi ,Hepatitis E virus, infekcija, dijagnostika, svinje, ljudi ,Hepatitis E virus, infection, diagnostic, pigs, humans ,infekcija ,Hepatitis E virus ,svinje ,diagnostic ,pigs ,humans ,dijagnostika ,infection - Abstract
Hepatitis E virus (HEV) je uzročnik akutne hepatis E infekcije kod ljudi. HEV se prenosi putem zagaĎene vode i odgovoran je za nastanak mnogobrojnih epidemija velikih razmera u zemljama u razvoju Azije i Afrike. Hepatitis E je prvi put kod svinja izolovan 1997. godine, a kasnije je dokazan i kod ostalih ţivotinjskih vrsta, kao što su: divlje svinje, jelen, zečevi, pacovi, ptice i ostalo.Prva istraţivanja prisustva HEV infekcije kod domaćih i divljih svinja u Srbiji sprovedena su 2008. godine. HEV RNK je dokazana u 30% uzoraka fecesa i 45% uzoraka organa (Petrovic et al., 2008). Analizom uzoraka krvnih seruma svinja u individualnim gazdinstvima ustanovljena je seroprevalencija od 34,6% (Lupulovic et al, 2010). Cilj ovog istraţivanja je ispitivanje raširenosti HEV infekcije kod svinja na farmama na teritoriji Vojvodine, kao i ispitivanje HEV seroprevalencije kod ljudi u Vojvodini.Od metoda za istraţivanje korišćene su: nekomercijalni ELISA test (in-house ELISA), komercijalni ELISA test, Western-blot metod, real-time RT -PCR i imunohistohemijska metoda za detekciju HEV antigena.Materijal za ispitivanje su bili uzorci krvi svinja (300) sa 3 farme na teritoriji Juţne Bačke i Srema i uzorci krvi ljudi (294), kao i uzorci fecesa, ţuči, jetre i mesa sakupljeni u klanicama od 95 tovljenika i 50 prasadi.Prisustvo specifičnih antitela IgG klase protiv hepatitis E virusa dokazano je kod svinja na sve tri ispitujuće farme. Primenom in house ELISA testa ustanovljena je seroprevalencija od 37% na farmi A, 31% na farmi B i 54% na farmi C, dok je primenom komercijalnog ELISA testa utvrĎeno 40% seropozitivnih svinja na farmi A, 41% na fami B i 65% na farmi C. Uporednom analizom rezultata dobijenih sa oba ELISA testa, ustanovljena je prosečna seroprevalencija od 40,66% in house ELISA testom, odnosno 48,66% komercijalnim ELISA testom.Sprovedena su i istraţivanja prisustva specifičnih antitela IgG klase protiv HEV u krvnim serumima dobrovoljnih davalaca krvi i kod pacijenata. Primenom in house ELISA testa utvrĎena jeseroprevalencija od 15% kod dobrovoljnih davalaca krvi, dok su uzorci krvi pacijenata bili seronegativni. Testiranjem komercijalnim ELISA testom kod dobrovoljnih davalaca krvi pozitivan serološki nalaz je ustanovljen kod 17,86% dobrovoljnih davalaca krvi (pregledani su serumi koji su u in house testu dali pozitivan ili sumnjiv nalaz, kao i odreĎen broj seronegativnih uzoraka), a kod pacijenata 2,12%.Kao tzv. „zlatni standard“ za definisanje rezultata sa sumnjivim serološkim nalazom čije su ekstinkcije bile blizu cut off vrednosti u in house ELISA testu, korišćen je Western blot metod. Pozitivan rezultat je potvrĎen kod 6 od ukupno pregledanih 11 uzoraka krvi svinja, odnosno od ukupno pregledanih 11 uzoraka seruma ljudi, pozitivan nalaz je ustanovljen kod 7 uzoraka.Uzorci sa klanica pregledani su real-time RT- PCR metodom, a HEV RNK je dokazana u u fecesu (54%), ţuči (26%), jetri (16%) i mesu (10%) prasadi. Kod tovljenika prisustvo HEV RNK je potvrĎeno samo u fecesu (7,27%), dok su svi uzorci tkiva bili negativni.Patahistološkim pregledom dokazane su mikroskopske lezije II stepena kod 3 uzorka (11,53%) jetri prasadi od ukupno pregledanih 26 uzoraka sa pozitivnim RT-PCR. Imunohistohemijskim pregledom uzoraka jetre prasadi nije dokazano prisustvo antigena hepatitis E virusa.Definisani su protokoli laboratorijskog ispitivanja hepatitis E infekcije kod svinja i ljudi, kao i u uzorcima mesa i jetri svinja u klanicama, Hepatitis E virus (HEV) is the causative agent of acute hepatitis E infection in humans. HEV is transmitted through contaminated water and is responsible for the outbreaks of many large-scale epidemics in the developing countries of Asia and Africa. Swine HEV was first isolated in 1997, and was later detected in other animal species, such are: wild boar, deer, rabbits, rats, birds and more.The first investigations of HEV infection in domestic and wild pigs in Serbia were carried out in 2008. HEV RNA was detected in 30% of faecal samples and 45% of the tissue samples (Petrovic et al., 2008). Analysing the blood samples of beckyard pigs, the seroprevalence of 34,6% was determined (Lupulovic et al, 2010). The aim of this study was to investigate the prevalence of HEV infection in pigs on farms in Vojvodina, as well as testing the HEV seroprevalence in humans.The methods used for this study were: non-commercial ELISA (in house ELISA), the commercial ELISA, Western blot method, real-time RT-PCR and immunohistochemical method for the detection of HEV antigen.Material for the study were: blood samples of pigs (300) from 3 farms on the territory of South Backa and Srem and blood samples of people (294), as well as faeces, bile, liver and meat collected in slaughterhouses from 95 fatteners and 50 piglets.The presence of specific IgG antibodies against the hepatitis E virus in pigs has been detected on all three examinated farms. Upon the application of in house ELISA, the seroprevalence of 37% was establised on farm A, 31% in farm B and 54% on farm C, while using a commercial ELISA , 40% of seropositive pigs were detected on farm A, 41% of fami B and 65% Farm C. The comparative analysis of the results obtained with both ELISA, determined the average seroprevalence of 40,66% by in house ELISA and 48,66% by commercial ELISA.The research of the presence of specific IgG antibodies against HEV in the serum of blood donors and patients were also conducted. Upon the application of in house ELISA, the seroprevalence of 15% were recorded in blood donors, while blood samples of patients were seronegative. Testing by commercial ELISA, positiv serological findings were diagnosed in 17,86% of blood donors (serums with positive or suspicious results in in house ELISA and a number of seronegative samples were tested), and in patients 2, 12%.As so-called "gold standard" for defining the serological results with suspiciousserological findings, which extinctions were close to cut off values in in house ELISA, we used the Western
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- 2013
35. PROPOSAL OF A PROGRAM FOR CONTROL, SUPPRESSION AND ERADICATION OF BOVINE VIRAL DIARRHEA VIRUS INFECTION IN CATTLE HERDS IN THE REPUBLIC OF SERBIA
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Petrović, Tamaš, primary, Lazić, Sava, additional, Bugarski, Dejan, additional, Lupulović, Diana, additional, Polaček, Vladimir, additional, Vidanović, Dejan, additional, and Plavšić, Budimir, additional
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- 2016
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36. FLAVIVIRUSES AT THE TERRITORY OF SERBIA - PRESENT SITUATION AND CHALLENGES.
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Petrović, Tamaš, Šekler, Milanko, Petrić, Dušan, Vidanović, Dejan, Potkonjak, Aleksandar, Cvjetković, Ivana Hrnjaković, Savić, Sara, Debeljak, Zoran, Lazić, Gospava, Ćupina, Aleksandra Ignjatović, Lupulović, Diana, Samojlović, Milena, Jurišić, Aleksandar, Petrović, Aleksandra, Ivanović, Ivana, Milošević, Vesna, and Lazić, Sava
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ZOONOSES ,FLAVIVIRUSES ,DISEASE prevalence ,ANIMAL species ,TICK-borne encephalitis - Abstract
Copyright of Archives of Veterinary Medicine / Arhiv Veterinarske Medicine is the property of Scientific Veterinary Institute Novi Sad and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
37. WEST NILE VIRUS SURVEILLANCE PROGRAM IN SERBIA
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Petrović, Tamaš, primary, Milenko, Šekler, additional, Petrić, Dušan, additional, Lazić, Sava, additional, Lupulović, Diana, additional, Lazić, Gospava, additional, Debeljak, Zoran, additional, Bugarski, Dejan, additional, and Plavšić, Budimir, additional
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- 2015
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38. SEROSURVEILLANCE OF EQUINE INFECTIOUS ANAEMIA IN A REGION OF VOJVODINA
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Vidić, Branka, primary, Savić, Sara, additional, Grgić, Živoslav, additional, Bugarski, Dejan, additional, Lupulović, Diana, additional, Prica, Nadežda, additional, and Marčić, Doroteja, additional
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- 2015
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39. Prevalence of hepatitis E virus (HEV) antibodies in Serbian blood donors
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Petrović, Tamaš, primary, Lupulović, Diana, additional, Jiménez de Oya, Nereida, additional, Vojvodić, Svetlana, additional, Blázquez, Ana-Belén, additional, Escribano-Romero, Estela, additional, Martín-Acebes, Miguel A, additional, Potkonjak, Aleksandar, additional, Milošević, Vesna, additional, Lazić, Sava, additional, and Saiz, Juan-Carlos, additional
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- 2014
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40. AUJESZKY'S DISEASE IN A DOG - CASE REPORT.
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Lazić, Gospava, Petrović, Tamaš, Lupulović, Diana, Topalski, Bojan, Božić, Biljana, and Lazić, Sava
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AUJESZKY'S disease ,VARIETY meats ,PIGLETS ,VETERINARIANS ,POLYMERASE chain reaction - Abstract
Copyright of Archives of Veterinary Medicine / Arhiv Veterinarske Medicine is the property of Scientific Veterinary Institute Novi Sad and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
41. Serological evidence of West Nile virus infection in the horse population of northern Serbia
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Medić, Strahinja, primary, Van den Hoven, Rene, additional, Petrović, Tamaš, additional, Lupulović, Diana, additional, and Nowotny, Norbert, additional
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- 2014
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42. STUDY OF THE EQUINE VIRAL ARTERITIS SEROCONVERSION AT HORSE STABLES IN THE TERRITORY OF VOJVODINA
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Lazić, Sava, primary, Lupulović, Diana, additional, Lazić, Gospava, additional, Živulj, Aleksandar, additional, Bugarski, Dejan, additional, and Petrović, Tamaš, additional
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- 2014
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43. Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia
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Toplak, Ivan, primary, Lazić, Sava, additional, Lupulović, Diana, additional, Prodanov-Radulović, Jasna, additional, Becskei, Zsolt, additional, Došen, Radoslav, additional, and Petrović, Tamaš, additional
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- 2012
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44. THE SEROPREVALENCE OF HEPATITIS E VIRUS INFECTION IN WILD BOARS IN SERBIA
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Lupulović, Diana, primary, Petrović, Tamaš, additional, Lazić, Sava, additional, Prodanov Radulović, Jasna, additional, Došen, Radoslav, additional, and Pušić, Ivan, additional
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- 2011
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45. EPIZOOTIOLOGICAL CHARACTERISTICS OF BOVINE TUBERCULOSIS IN SOUTH BAČKA REGION
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Pušić, Ivan, primary, Lalošević, Dušan, additional, Bugarski, Dejan, additional, Prodanov, Jasna, additional, Grgić, Živoslav, additional, Urošević, Miroslav, additional, and Lupulović, Diana, additional
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- 2009
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46. SEROLOGICAL EXAMINATION OF PARVOVIRUS INFECTION ON PIG FARMS
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Lupulović, Diana, primary, Lazić, Sava, additional, Petrović, Tamaš, additional, and Prodanov, Jasna, additional
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- 2009
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47. TESTING THE PREVALENCE OF BVDV INFECTION IN LARGE AND SMALL CATTLE HERDS IN SOUTH BACKA AND SREM DISTRICT
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Petrović, Tamaš, primary, Lazić, Sava, additional, Lupulović, Diana, additional, Bugarski, Dejan, additional, and Đuričić, Bosiljka, additional
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- 2008
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48. PREVALENCE OF HERPESVIRUS 1 IN SMALL HERDS IN SOUTHERN BAČKA AND SREM DISTRICT
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Lazić, Sava, primary, Petrović, Tamaš, additional, Lupulović, Diana, additional, Bugarski, Dejan, additional, Pušić, Ivan, additional, Polaček, Vladimir, additional, and Maljković, Marko, additional
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- 2008
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49. Comparative examination of antibodies against lumpy skin disease virus by enzyme-linked immunoassay and virus neutralization test
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Samojlović, Milena, Rogan, Dragan, Lazić, Sava, Radinović, Miodrag, Novakov, Nikolina, and Lupulović, Diana
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Bolest kvrgave kože, goveda, antitela, ELISA, VNT, imunitet, pasivni imunitet ,immunity ,goveda ,antitela ,pasivni imunitet ,passive immunity ,Lumpy skin disease, cattle, antibodies, ELISA, VNT, immunity, passive immunity ,cattle ,Bolest kvrgave kože ,VNT ,antibodies ,ELISA ,Lumpy skin disease ,imunitet - Abstract
Bolest kvrgave kože (lumpy skin disease – LSD) je virusno oboljenje goveda, veoma važno sa ekonomskog aspekta, zbog velikih gubitaka u govedarskoj proizvodnji. Brzi i pouzdani laboratorijski testovi su neophodni, kako za rano otkrivanje bolesti i utvrđivanje statusa zapata, tako i za praćenje imunološkog statusa jedinki nakon vakcinacije, koja je i dalje najsigurnija mera u borbi protiv ove bolesti. Radi sprovođenja efikasnih mera kontrole protiv LSD, kao što su pravovremena vakcinacija, naročito teladi i serološki nadzor zapata, neophodno je vršiti istraživanja vezana za postvakcinalni imunološki odgovor, kako kod odraslih jedinki, tako i kod teladi. Cilj ove doktorske distertacije je praćenje antitela protiv virusa bolesti kvrgave kože (lumpy skin disease virus – LSDV) kod vakcinisanih krava, praćenje perzistencije maternalnih antitela kod teladi poreklom od vakcinisanih krava, kao i sprovođenje postupka validacije i potvrde primene modifikovanog virus neutralizacionog testa razvijenog na Odeljenju za virusologiju Naučnog instituta za veterinarstvo „Novi Sad“. Materijal za ispitivanje korišćen u ovom istraživanju potiče od goveda sa područja u kojima nije registrovana bolest tokom epizootije u Srbiji 2016. godine (Južnobački, Sremski i Južnobanatski okrug), a vakcinacija goveda protiv bolesti kvrgave kože je počela da se sprovodi tokom jula meseca 2016. godine. Uporedno ispitivanje je izvršeno na ukupno 355 uzoraka krvnih seruma krava, 15 uzoraka kolostruma i 270 uzoraka krvnih seruma teladi. Za uporedno ispitivanje prisustva antitela protiv LSDV u uzorcima korišćene su metode ELISA i virus neutralizacioni test (VNT). ELISA test je izvođen korišćenjem komercijalnog kita ID Screen® Capripox Double Antigen Multispecies proizvođača IDvet (France), dok je VNT izvođen upotrebom kulture ćelija Madin-Darby bovine kidney (MDBK) i virusa bolesti kvrgave kože poreklom od klinički obolelog govečeta u trajanju od 3 dana. Prvo prisustvo specifičnih antitela protiv LSDV u krvnim serumima vakcinisanih krava utvrđeno je 20 dana nakon vakcinacije, najviši nivo serokonverzije utvrđen je 30 dana nakon vakcinacije, a prisustvo antitela je bilo moguće detektovati tokom četiri meseca nakon vakcinacije, modifikovanim VNT kod 34% krava, a ELISA testom kod 30% vakcinisanih krava. Prisustvo specifičnih antitela protiv LSDV ELISA metodom je bilo moguće utvrditi 90 dana nakon teljenja kod 16,67% teladi, 105 dana nakon teljenja kod 10% teladi, a uposlednjem terminu uzorkovanja, 120 dana nakon teljenja, kod svega 6,67% teladi. VNT je u istim terminima prisustvo specifičnih antitela bilo moguće utvrditi kod 10%, 6,67%, i 3,33% teladi. Ni u jednom terminu uzorkovanja nije bilo moguće utvrditi prisustvo antitela protiv LSDV kod svih ispitanih teladi. Na dan teljenja je prisustvo specifičnih antitela protiv LSDV utvrđeno u 63,33% uzoraka krvnih seruma krava ELISA metodom, u 73,33% uzoraka krvnih seruma krava metodom VNT, dok je obema metodama prisustvo antitela utvrđeno u 86,67% uzoraka kolostruma. Statističkom analizom je utvrđen značajno veći nivo antitela u kolostralnom u odnosu na krvni serum krava. Rezultati uporednog ispitivanja modifikovanog VNT i komercijalnog ELISA testa za detekciju antitela protiv LSDV u uzorcima krvnih seruma iz banke seruma i vakcinisanih krava pokazali su skoro savršenu saglasnost poređenih metoda (k=0,913). S druge strane, visoka saglasnost poređenih metoda (k=0,7239) je postignuta kod uporednog ispitivanja modifikovanog VNT i komercijalnog ELISA testa za detekciju antitela protiv LSDV u uzorcima krvnih seruma vakcinisanih krava i njihove teladi. Ispitivanjem uzoraka krvnih seruma iz banke seruma goveda pre pojave LSD u Republici Srbiji izračunata je specifičnost modifikovanog VNT od 100%, a komercijalnog ELISA testa od 99,2%. S obzirom da je VNT zlatni standard serološke dijagnostike LSD, izračunata je osetljivost komercijalnog ELISA testa u odnosu na modifikovani VNT za detekciju antitela u uzorcima krvnih seruma vakcinisanih krava od 88,24% i za detekciju antitela protiv LSDV u uzorcima krvnih seruma vakcinisanih krava i njihove teladi od 86,44%. Rezultati ovog istraživanja su pokazali da se modifikovani VNT i komercijalni ELISA test (proizvođača „IDvet“) mogu koristiti za detekciju antitela protiv LSDV. Modifikovani VNT se pokazao kao jednostavniji i brži za izvođenje u odnosu na preporučeni VNT od strane OIE. Datum, Lumpy skin disease (LSD) is a viral disease of cattle, very important from an economic aspect, due to significant losses that can cause in livestock production. Rapid and reliable laboratory tests are necessary, both for early detection of disease and determination of the status of the herds, as well as for monitoring of the immune status of individual animals after vaccination, which is still the most effective measure for controlling the spread of LSD. In order to implement effective LSD control measures, such as timely vaccination, particularly in calves and serological monitoring, it is necessary to carry out researches related to postvaccinal immune response, both in adult cattle and in their calves. The goal of this doctoral dissertation is to monitor the presence of antibodies against lumpy skin disease virus (LSDV) in vaccinated cows, to monitor the persistence of maternal antibodies in calves born to vaccinated cow, as well as to carry out the validation process and confirm the use of the modified virus neutralization test developed at the Department of virology of the Scientific veterinary institute "Novi Sad", Serbia. The material for testing used in this study originated from cattle in areas where no LSD outbreaks were registered during the epizootic in Serbia in 2016 (South Bačka, Srem and South Banat Districts), and the vaccination of cattle against LSD started in July 2016. A comparative examination was carried out on a total of 355 cow blood sera samples, 15 colostrum samples and 270 calf blood sera samples. ELISA and virus neutralization test (VNT) were used for comparative examination of samples on the presence of antibodies against LSDV. ELISA test was performed using the commercial set kit ID Screen® Capripox Double Antigen Multi-species manufactured by IDvet (France), while a 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow. The first presence of specific antibodies against LSDV in vaccinated cows was detected 20 days after vaccination, the highest seroconversion was determined 30 days after vaccination, and the presence of antibodies against LSDV could be detected during four months after vaccination by VNT and commercial ELISA in 34% and 30% of vaccinated cattle, respectively. The presence of specific antibodies against the LSDV by ELISA method could bedetermined 90 days after calving in 16.67% of calves, 105 days after calving in 10% of calves, and in the last sampling interval, 120 days after calving, in only 6.67% of calves. In above-mentioned sampling intervals, the presence of specific antibodies by VNT could be determined in 10%, 6.67%, and 3.33% of calves. In any sampling period we did not determined that all calves were seropositive to LSDV. On calving day, the presence of specific antibodies against LSDV was detected in 63.33% of cow blood sera samples by ELISA, in 73.33% of cow blood sera samples by VNT method, while the presence of antibodies was found in 86.67% of colostrum samples by both methods. A statistical analysis showed a significantly higher level of antibodies in the colostrum compared to the cow blood sera. The results of comparative examination of modified VNT and commercial ELISA test for the detection of antibodies against LSDV in blood sera samples from the sera bank and of vaccinated cows showed an almost perfect agreement of the compared methods (k = 0.913). On the other hand, the substantial agreement of the compared methods (k = 0.7239) was achieved in a comparative examination of modified VNT and commercial ELISA test for the detection of antibodies against LSD in blood sera samples of vaccinated cows and their calves. The specificity of modified VNT and commercial ELISA was 100% and 99.2% respectively. It was calculated by testing blood sera samples from sera bank before the occurrence of LSD in the Republic of Serbia. Since VNT is “the gold standard” serological test for LSDV, the sensitivity of commercial ELISA in relation to modified VNT was calculated for the detection of antibodies in blood sera samples of vaccinated cows of 88.24% and for the detection of antibodies against LSDV in blood sera samples of vaccinated cows and their calves of 86.44%. The results of this study showed that modified VNT and commercial ELISA manufactured by „IDvet“ can be used for the detection of antibodies against LSDV. Modified VNT proved to be simpler to perform and to take less time compared to the recommended VNT by the OIE.
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- 2019
50. Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve "Zasavica", Serbia.
- Author
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Lazić S, Savić S, Petrović T, Lazić G, Žekić M, Drobnjak D, and Lupulović D
- Abstract
The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in "Zasavica", blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test).
- Published
- 2023
- Full Text
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