1. [Biospecific preparation and some properties of molecular factors, interacting with brain-specific proteins S100].
- Author
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Poletaev AB, Beliaev SV, and Lysova NP
- Subjects
- Animals, Calcium pharmacology, Cattle, Ligands, Oligopeptides analysis, Organ Specificity, Proteins metabolism, S100 Proteins metabolism, Brain metabolism, Liver metabolism, Nerve Tissue Proteins isolation & purification, Proteins isolation & purification, S100 Proteins isolation & purification
- Abstract
The biospecific preparation and a preliminary analysis of physico-chemical properties of water-soluble proteins from bovine brain and liver and brain oligopeptides specifically adsorbed on a column with immobilized brain-specific proteins of the major fraction S100 were carried out. Using thin-layer electrophoresis on cellulose and tachyphoresis, it was found that four oligopeptide cations and three anionic oligopeptides interact with S100. Polyacrylamide gel electrophoresis revealed at least six brain proteins and 11 liver proteins, which interact with S100. An essential role in interaction of protein ligands from the bran (but not from liver) with immobilized S100 proteins belongs to Ca2+. The use of natural endogenous ligands for the study of the biological role of brain-specific proteins S100 is discussed.
- Published
- 1982