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Objective: To determine the E. faecalis biofilm formation on the surface of five adhesive systems (AS) and its relationship with roughness. Study Design: The formation of E. faecalis biofilms was tested on the surface of four dual-cure AS: AdheSE DC, Clearfil DC Bond, Futurabond DC and Excite DSC and one light-cure antimicrobial AS, Clearfil Protect Bond, after 24 hours of incubation, using the MBEC high-throughput device. Results: E. faecalis biofilms grew on all the adhesives. The least growth of biofilm was on Excite DSC, Clearfil Protect Bond, and the control. Futurabond DC resulted in the greatest roughness and biofilm amount. There was a close relationship between the quantity of biofilm and roughness, except for Clearfil Protect Bond, which showed little biofilm but high roughness. Conclusion: None of the tested AS prevented E. faecalis biofilm formation, although the least quantity was found on the surface of Clearfil Protect Bond. Key words:Adhesive systems, biofilm, Enterococcus faecalis, roughness.

Currently, the tooth bleaching became a procedure very looked and used in the clinic dental, in function of the aesthetic valuation. The decision of bleaching always comes of the aesthetic necessity of the patient. The objective in this work is to present a clinical case of internal and external bleaching of pulped teeth being approached the materials, the technique and the cares that we must take in the hour of the intervention, so that let us can get the desired success. The treatment consisted of the association of the perborato of sodium with distilled water (folder), also showing the cares that if must have at the moment of the procedure. The clinical results had allowed to conclude that the efficiency of the use of the water distilled in substitution to hydrogen peroxide if makes gift, beyond the refined criterion in the choice of the bleaching treatment.

At reporting of any clinical case, it always would be interesting to point out the many reasons that gave support to the chosen treatment options, during all the developed steps, as expose the reasons that were used as a base to the choice of the employed procedures. With this philosophical focus, it is here presented a clinical case, involving substitution of amalgam by composite resin, in two upper left tooth from the posterior region (24 and 25), realized in a male patient that was treated in the Pos-graduation Clinic, at Bauru Dental School - USP. In the two involved premolars, the defective amalgam was substituted by Filtek Supreme, a composite resin, after realization of a general clinical evaluation, followed by anamnesis and analysis of the respective initial radiographies. In the way, besides a detailed description of clinical case, it were pointed out the reasons that served as basis to the choices of different procedures, beginning with explanation of why composite was elected to substitute amalgam.

Dental cements must present satisfactory mechanical properties in order to support masticatory forces and occlusal loads in the oral cavity. The punch shear strength is a simple and efficient method to evaluate the strength of a material, by means of an axial load applied to dislocate one or several materials. Thus, the objective of this work was to evaluate three dental cements (a zinc phosphate, a glass-ionomer, and a resin cement) as to their punch shear strength. For this test, specimens in a ring form matrix with dimensions of 14mm of internal diameter and 1.5mm of height were made, allowing for a disc of the materials with these dimensions to be obtained, which was placed in an adequate testing device. Ten specimens of each material were analyzed for the respective test at periods of 1 and 24 hours. The results were statistically evaluated with the two-way ANOVA test, followed by the comparative Tukey’s test (5%). The dental cement which obtained the highest values of punch shear strength was the resin cement, whereas the glass-ionomer cement obtained the lowest values among all the analyzed materials in both periods.

The purpose of this study was to evaluate the accuracy of the respective dies after polyether elastomeric procedure in the presence or absence of cervical contact of the acrylic resin shell with the cervical region, establishing a comparison to dies obtained with stock trays. This study consisted of three groups with 10 specimens each: 1) acrylic copings without cervical contact, (cn); 2) acrylic copings with cervical contact (cc); 3) perforated stock tray, (st). The accuracy of the resulting dies was verified with the aid of a master crown, precisely fit to the master steel die. ANOVA test found statistically significant differences among groups (p

Neste estudo, foi avaliada a resistência ao desgaste abrasivo de uma resina composta, através de três diferentes métodos: do disco retificado, por escovação e do nanodurômetro. Nos dois primeiros métodos, foram utilizados 20 espécimes da resina Filtek Z250 (10 da cor Incisal e 10 da cor C4, ambos fotopolimerizados por 30 segundos cada) e 10 de polimetilmetacrilato (PMMA), atuando estes últimos como padrão de referência. Para os ensaios com o nanodurômetro, foi utilizado apenas um espécime de cada cor, assim como um único de PMMA, em cujas superfícies planas foram efetuadas sete medições, o que foi considerado equivalente a sete espécimes. No método do disco retificado, cada espécime de resina composta era confeccionado diretamente numa cavidade semicircular, localizada na periferia de um disco-suporte; os espécimes de PMMA consistiam em discos constituídos exclusivamente por este material; o desgaste de cada espécime era promovido por um disco de porcelana e seu conseqüente deslocamento era detectado por um palpador; assim, a taxa de desgaste era estabelecida, expressa em milímetros cúbicos por newton por segundo (mm3/N.s). No método de escovação, cada espécime cilíndrico ficava preso numa placa metálica, a qual era fixada no interior da máquina de escovação; sua perda de massa (em miligramas) era verificada através de uma balança analítica. No método do nanodurômetro, existiu apenas um espécime ci líndrico para cada condição, em cuja face plana superior eram feitos sete sulcos (equivalentes a sete espécimes); o coeficiente de atrito permitia estabelecer a respectiva resistência ao desgaste. Para cada método utilizado, os dados foram tratados estatisticamente, através de análise de variância (ANOVA) e de teste de Tukey. Pôde-se concluir que, através do disco retificado, a taxa de desgaste (em mm3/N.s) dos materiais foi: Incisal (0,6807) > C4 (0,5012) > PMMA (0,1439); que, através da escovação, a taxa de desgaste (em mg) foi de 5,40 para Incisal, de 9,35 para C4 e de 5,88 para PMMA, sem diferença significante apenas entre Incisal e PMMA, os mais resistentes; e que, através do nanodurômtero, a resistência ao desgaste abrasivo (expressa pelo coeficiente de atrito) foi de 0,207 para Incisal, de 0,214 para C4 e de 0,281 para PMMA, sem diferença significante agora apenas entre Incisal e C4, os menos resistentes. Abrasive wear of a composite resin, using three different methods (ground disc, toothbrushing and nanodurometer). The aim at this study was to evaluate the resistance to abrasive wear of a composite resin, using three different methods: ground disc, toothbrushing and nanodurometer. At the first two methods were employed 20 specimens of Filtek Z250 composite resin (10 of Incisal and 10 of C4, both the colors cured for 30 seconds each one) and 10 of polymethylmethacrylate (PMMA), the latter acting as a reference standard. For the tests with the nanodurometer it was used only one specimen of each color, as well as just one of PMMA, in whose flat surfaces seven measurements were made, what was considered equivalent to seven specimens. At the ground disc method, each composite resin specimen was directly made on a semicircular cavity located on the periphery of a supporting disc; PMMA specimens consisted of discs made up exclusively with this material; the wear of each specimen was promoted by a ceramic disc and its consequent displacement was detected by a sensor; thus, the wear rate was established, expressed in cubic millimeters per newton per second (mm3/N.s). At the toothbrushing method, each cylindrical specimen was trapped in a metal plate, which was fixed inside the machine brushing; its weight loss (in milligrams) was verified by an analytical balance. At the nanodurometer method, there was only one cylindrical specimen for each condition, in whose flat upper face seven grooves were made (what was equivalent to seven specimens); the coefficient of friction was used to establish its wear resistance. For each method, data were statistically analyzed by ANOVA and Tukey\'s test. It could be concluded that, by ground disc method, materials wear rate (in mm3/N.s) was Incisal (0.6807) > C4 (0.5012) > PMMA (0.1439); by brushing method, materials wear rate (in mg) was 5.40 for Incisal, 9.35 for C4 and 5.88 for PMMA, without significant difference only between Incisal and PMMA (the toughest); by nanodurometer method, abrasive wear resistance (expressed by the coefficient of friction) was 0.207 for Incisal, 0.214 for C4 and 0.281 for PMMA, now without significant difference just between Incisal and C4, the least resistant.

O objetivo neste trabalho foi avaliar a influência da variação do tempo de fotopolimerização para diferentes cores (I, B1, B3 e C4) de uma resina composta (FiltekTM Z250, da 3M ESPE), sobre a taxa de desgaste abrasivo através do método do disco retificado. Cada espécime de resina composta fotopolimerizada estava contido numa cavidade semicircular localizada na periferia de um disco constituído por polimetilmetacrilato (PMMA), denominado disco estático, sendo que a superfície curva da resina no disco sofria desgaste por um rebolo de porcelana, denominado tecnicamente de disco dinâmico, sob carga padronizada e velocidade controlada, criando no espécime uma cavidade de aspecto semicircular. O deslocamento vertical do disco estático, ao ser desgastado, era detectado por um palpador, o qual transferia estes dados a um computador e criava os arquivos de desgaste, em função do tempo. Os dados obtidos foram tratados estatisticamente. Pôde-se concluir que 1) a resistência à abrasão do PMMA foi a maior de todas e 2) a resina de cor C4, a mais resistente de todas. The aim at this study was estimate the influence of light-curing time variation of different colors (I, B1, B3 and C4) of composite resin (FiltekTM Z250, 3M ESPE), upon abrasive wear through the retified disc method. The porcelain existent in the named dynamic disc promoted a wear of each resin specimen located in the polimethylmetacrylate (PMMA) static disc, under a standardized charge and with controlled velocity. Vertical displacement of static disc was detected by a sensor that send data to a computer where wear arquives were plotted in function of time. After data statistical analysis, it could be concluded that: 1) PMMA resistance was greater than anyone of resins and 2) C4 was more resistant than all the other colors.

Introduction: Stone casts are used in dentistry to allow the confection of indirect restorations. Thus, gypsum dimensional stability is fundamental in order to achieve a precise fit between dental structure and restorative material. Objective: The purpose of the present study was to evaluate the hygroscopic linear expansion of commercially available gypsum products. Material and methods: Fifteen dental stones were analyzed (4 type II, 5 type III, 4 type IV and 2 type V), with n = 5. One hundred grams of each gypsum product were mixed following the respective manufacturers’ recommendations for powder/water ratio and poured into a V-shape mold, connected to an expansion-measuring device. The hygroscopic expansion was evaluated during 120 min and the collected data was converted into percentage and compared to initial dimensions of the specimen. Results: All type II and V dental stones showed expansion values compatible to the ADA and ISO recommendations. Considering type III stones, only Rutenium 3 was compatible with the recommendations. For type IV, only Durone IV showed expansion values higher than that recommended by ADA, but lower than that of ISO recommendations. Conclusion: The hygroscopic linear expansion of gypsum products should be periodically controlled in order to ensure their precision and reliability to dental practice.

Objective: To evaluate, in vitro, the antimicrobial activity and biofilm formation of three chlorhexidine varnishes in four Enterococcus faecalis strains: E. faecalis ATCC 29212, E. faecalis EF-D1 (from failed endodontic treatment), E. faecalis 072 (cheese) and E. faecalis U-1765 (nosocomial infection), and one Enterococcus durans strain (failed endodontic treatment). Study Design: The direct contact test was used to study the antimicrobial activity. Bacterial suspensions were exposed for one hour to EC40, Cervitec (CE) and Cervitec Plus (CEP) varnishes. “Eradication” was defined as 100% bacterial kill. The formation of enterococci biofilms was tested on the surface of the varnishes after 24 hours of incubation and expressed as percentage of biofilm reduction. Results: EC40 eradicated all strains except E. faecalis ATCC 29212, where 98.78% kill was achieved. CE and CEP showed antimicrobial activity against all the strains, but most clearly against E. durans and E. faecalis 072. EC40 completely inhibited the formation of biofilm of E. faecalis ATCC 29212, E. faecalis 072 and E. durans. CE and CEP led to over 92% of biofilm reduction, except in the case of E. faecalis U-1765 on CEP (76.42%). Conclusion: The three varnishes studied were seen to be effective in killing the tested strains of enterococci and in inhibiting the formation of biofilm, the best results being observed with EC40. Key words:Biofilm, chlorhexidine varnish, direct contact test, Enterococcus durans, Enterococcus faecalis, intracanal medication.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:01:46Z No. of bitstreams: 1 S0104-14282007000300017.pdf: 363571 bytes, checksum: f6b9ca282437aa9221fa2e6b4397cd29 (MD5) Made available in DSpace on 2013-08-22T19:01:46Z (GMT). No. of bitstreams: 1 S0104-14282007000300017.pdf: 363571 bytes, checksum: f6b9ca282437aa9221fa2e6b4397cd29 (MD5) Previous issue date: 2007-09-01 Made available in DSpace on 2013-09-30T20:02:06Z (GMT). No. of bitstreams: 2 S0104-14282007000300017.pdf: 363571 bytes, checksum: f6b9ca282437aa9221fa2e6b4397cd29 (MD5) S0104-14282007000300017.pdf.txt: 26473 bytes, checksum: 8e8886747ea777cbdf3282d7ec8148bd (MD5) Previous issue date: 2007-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:27:20Z No. of bitstreams: 2 S0104-14282007000300017.pdf: 363571 bytes, checksum: f6b9ca282437aa9221fa2e6b4397cd29 (MD5) S0104-14282007000300017.pdf.txt: 26473 bytes, checksum: 8e8886747ea777cbdf3282d7ec8148bd (MD5) Made available in DSpace on 2014-05-20T13:27:20Z (GMT). No. of bitstreams: 2 S0104-14282007000300017.pdf: 363571 bytes, checksum: f6b9ca282437aa9221fa2e6b4397cd29 (MD5) S0104-14282007000300017.pdf.txt: 26473 bytes, checksum: 8e8886747ea777cbdf3282d7ec8148bd (MD5) Previous issue date: 2007-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O propósito deste trabalho é fazer uma comparação entre resinas poliméricas dentárias, polimerizadas por aparelhos baseados em lâmpada halógena e diodo emissor de Luz (LED), utilizando-se o método do disco retificado aperfeiçoado para odontologia e os respectivos valores de microdureza. Foram realizados testes em amostras de resinas compostas de 5 diferentes marcas, polimerizadas a tempos de 10, 20 e 40 s, pelos dois aparelhos. A análise estatística dos valores de microdureza e agressividade permitiu concluir que estatisticamente não há correlação entre essas propriedades. Na análise de microdureza, a heterogeneidade característica do material implicou em resultados com valores de desvio padrão relativamente altos, de forma que não foi encontrada diferença estatística entre as amostras avaliadas. Na análise estatística dos ensaios baseados no método do disco retificado, a resina que apresentou maior desgaste nos ensaios, foi a Tetric Ceram, polimerizada pelo aparelho de LED por 10 s, cujo valor médio de agressividade obtido foi 0,170 mm³/N.m. A resina que sofreu menor desgaste foi a Charisma, polimerizada por Lâmpada Incandescente, por um tempo de 20 s, cuja média dos valores de agressividade foi 0,057 mm³/N.m. The purpose of this work was to compare polymeric dental resins cured with halogen lamp and with light emission diode (LED) devices, using the grinding disk method customized for dentistry and the corresponding microhardness values. Tests were carried out on resin samples of five brands, which were cured for 10, 20 and 40 s with the two devices. The analysis of microhardness and aggressiveness has allowed us to conclude that there is no correlation between these properties. In Microhardness tests, the material heterogeneity has produced relative high standard deviation values and has not shown statistical differences between the analyzed samples. In the statistical analysis for the results with the grinding disk method, the resin with highest wear was the Tetric Ceram, cured with LED device for 10 s (aggressiveness average 0.170 mm³/N.m ). The composite resin with least wear was Charisma, cured with halogen lamp device for 20 s (aggressiveness average 0.057 mm³/N.m ). UNESP Faculdade de Engenharia de Bauru USP Faculdade de Odontologia de Bauru UNESP Faculdade de Engenharia de Bauru

OBJETIVO: Caracterizar mulheres e recém-nascidos que foram submetidos ao processo de reprodução assistids. MÉTODO: Estudo descritivo, retrospectivo para coleta de dados e prospectivo para análise, em mulheres submetidas a duas diferentes técnicas de reprodução assistida no Centro de Reprodução Humana do Hospital Israelita Albert Einstein no período de janeiro de 1995 a Dezembro de 2003. Foram selecionados todos os casos de sucesso e término do parto que ocorreram na Instituição no período. RESULTADOS: no período analisado, ocorreram 2448 procedimentos, sendo 439 de fertilização in vitro e 2009 de injeção intracitoplasmática de esperma. A taxa de sucesso variou de 25 a 30%. Não houve diferenças significativas entre os dois métodos estudados quanto aos resultados perinatais. Maior morbidade foi observada entre os nascimentos múltiplos em relação aos únicos. CONCLUSÕES: as técnicas de reprodução assistida não interferiram nos resultados perinatais. A prematuridade foi o fator determinante de maior morbimortalidade entre os múltiplos.

Buscou-se, por meio de uma revisão bibliográfica de publicações científicas, atualizar conhecimentos produzidos sobre reprodução assistida. Inicialmente, investigou-se o histórico e, em seguida, foram apresentados conceitos relativos à infertilidade e sobre as técnicas de reprodução assistida, especificando-se ainda alguns temas relacionados a aspectos obstétricos, epidemiológicos e perinatais. Vários artigos que abordam o tema foram apresentados. Foi discutida a situação no Brasil bem como os vários aspectos assinalados.

CONTEXT: During the first year of life, the growth process is highly vulnerable to several impairing factors that need to be understood. OBJECTIVE: To perform follow-up evaluation on newborns weighing less than or equal to 2,000 g in a population of low socioeconomic level. TYPE OF STUDY: Retrospective. SETTING: Hospital Maternidade Escola de Vila Nova Cachoeirinha, São Paulo, Brazil. METHODS: The study included 60 children born between March 1996 and January 1998, weighing less than or equal to 2,000 g. They were divided into three subgroups, according to birth weight and adequacy for gestational age. The factors studied were maternal variables, illnesses among the newborns, hospital admissions subsequent to discharge from the nursery, and the evolution of weight from birth until 12 months of life. Statistical analyses were performed through application of the Statistical Package for Social Sciences (SPSS) V.9.0 and Curve Expert 1.3 programs. RESULTS: Previous maternal diseases occurred in 38.6% of the pregnant women and intercurrent events occurred in 100%. The prevailing neonatal diseases were sepsis (30%) and hyaline membrane disease (25%). There were 404 visits on an outpatient basis: the most frequently diagnosed complaints related to respiratory diseases (26%). Among visits to specialists, 81.7% were to the neuropediatrician. A diagnosis of normality was made for 80% of all visits, for all specialties. For each of these groups, a growth curve was established. These were shown to be below the reference curve standards, with such differences least evident with regard to the children's corrected age. DISCUSSION: The severity of the newborns'conditions may be related to the high incidence of maternal diseases prior to pregnancy as well as intercurrent events during pregnancy. The differences in growth in relation to NCHS charts show that corrected age should be used as a parameter. CONCLUSIONS: Socioeconomic conditions, clinical/obstetric events and newborn diseases during the hospital stay had repercussions on these children's progress during their first year of life. Their growth profile was found to be very far from the reference standard, thus indicating a need for constant, differentiated assessment. CONTEXTO: O processo de crescimento durante o primeiro ano de vida é altamente vulnerável a múltiplos fatores que podem prejudicá-lo, o que torna necessário seu conhecimento e controle rigoroso. OBJETIVO: Avaliar o acompanhamento de recém-nascidos com peso de nascimento < 2.000 g, de uma população de periferia de grande cidade, durante o primeiro ano de vida. TIPO DE ESTUDO: Retrospectivo, a partir de dados secundários. LOCAL: Hospital Maternidade Escola de Vila Nova Cachoeirinha, São Paulo, Brasil. MÉTODOS: 60 recém-nascidos com peso < 2.000 g, divididos em três subgrupos conforme peso e adequação à idade gestacional, foram estudados de março de 1996 a janeiro de 1998. Variáveis maternas, variáveis relativas ao seguimento das crianças no primeiro ano de vida, às admissões após a alta hospitalar e relativas ao peso durante o primeiro ano de vida foram estudadas. As análises estatísticas utilizaram o programa SPSS (Statistical Package for Social Sciences, SPSS) V.9,0 e o Curve Expert 1.3. RESULTADOS: Doenças maternas prévias ocorreram em 38,6% e em 100% dos casos houve intercorrências durante a gravidez. Das doenças neonatais, as de maior prevalência foram sepse (30%) e doença de membranas hialinas (25%). No ambulatório realizaram-se 404 consultas e as doenças respiratórias corresponderam ao diagnóstico de maior freqüência (26%). Das consultas com especialistas, aquelas com o neuropediatra corresponderam a 81,7%. Em 80% das consultas em todas as especialidades fez-se o diagnóstico de normalidade. Foi construída uma curva de crescimento para cada grupo e feitas comparações com as curvas de referência do National Center for Health Statistics que se mostraram abaixo dos padrões das curvas de referência, diferença menos evidente para a idade corrigida das crianças. DISCUSSÃO: O serviço onde foi realizado este estudo é referência para gestantes de alto risco de baixo nível socioeconômico. A gravidade das condições dos recém-nascidos pode estar relacionada com a alta incidência de doenças maternas antes da gravidez e intercorrências durante a gestação. As diferenças no crescimento em relação aos padrões do NCHS mostram que a idade corrigida deveria ser usada como parâmetro de crescimento para recém-nascidos de baixo peso. CONCLUSÕES: Condições socioeconômicas, eventos clínico-obstétricos e afecções apresentadas pelos recém-nascidos durante a internação repercutiram sobre sua evolução no primeiro ano de vida e seu perfil de crescimento distanciou-se do padrão referencial indicando necessitarem de avaliação constante e diferenciada.