Search

International audience; INTRODUCTION: With the spread of COVID-19 epidemic, health plans must be adapted continuously. There is an urgent need to define the best care courses of patients with COVID-19, especially in intensive care units (ICUs), according to their individualised benefit/risk ratio. Since older age is associated with poorer short-term and long-term outcomes, prediction models are needed, that may assist clinicians in their ICU admission decision. Senior-COVID-Rea was designed to evaluate, in patients over 60 years old admitted in ICU for severe COVID-19 disease, the impact of age and geriatric and paraclinical parameters on their mortality 30 days after ICU admission. METHODS AND ANALYSIS: This is a multicentre survey protocol to be conducted in seven hospitals of the Auvergne-Rhône-Alpes region, France. All patients over 60 years old admitted in ICU for severe COVID-19 infection (or their legally acceptable representative) will be proposed to enter the study and to fill in a questionnaire regarding their functional and nutritional parameters 1 month before COVID-19 infection. Paraclinical parameters at ICU admission will be collected: lymphocytes and neutrophils counts, high-fluorescent lymphoid cells and immature granulocytes percentages (Sysmex data), D-dimers, C-reactive protein, lactate dehydrogenase (LDH), creatinine, CT scan for lung extension rate as well as clinical resuscitation scores, and the delay between the first signs of infection and ICU admission. The primary outcome will be the overall survival at day 30 post-ICU admission. The analysis of factors predicting mortality at day 30 will be carried out using univariate and multivariate logistic regressions. Multivariate logistic regression will consider up to 15 factors.The ambition of this trial, which takes into account the different approaches of geriatric vulnerability, is to define the respective abilities of different operational criteria of frailty to predict patients' outcomes. ETHICS AND DISSEMINATION: The study protocol was ethically approved. The results of the primary and secondary objectives will be published in peer-reviewed journals. TRIAL REGISTRATION NUMBER: NCT04422340.

Methods for the radiolabeling of biologics with fluorine-18 have been of interest for several decades. A common approach consists in the preparation of a prosthetic reagent, a small molecule bearing a fluorine-18 that is conjugated with the macromolecule to an appropriate function. Click chemistry, and more particularly cycloadditions, is an interesting approach to radiolabel molecules thanks to mild reaction conditions, high yields, low by-products formation, and strong orthogonality. Moreover, the chemical functions involved in the cycloaddition reaction are stable in the drastic radiofluorination conditions, thus allowing a simple radiosynthetic route to prepare the prosthetic reagent. We report herein the radiosynthesis of 18 F-FPyZIDE, a pyridine-based azide-bearing prosthetic reagent. We exemplified its conjugation via copper-catalyzed cycloaddition (CuAAC) and strain-promoted cycloaddition (SPAAC) with several terminal alkyne or strained alkyne model compounds.

Background Nowadays, more people are treating dogs as family members. This reflects their increased attention towards their nutrition, with renewed interest for non-conventional diets such as Biologically Appropriate Raw Food/ Bones and Raw Food in United States (BARF) or homemade. In previous studies, owners feeding their dog non-conventional diets reported lower levels of trust in veterinary advice. The aim of the study was to identify differences in lifestyle between owners feeding dogs non-conventional diets and those feeding conventional diets (i.e., dry/wet pet food) to give further insight for improving communication between veterinarians and owners. Results A total of 426 surveys were usable. Fifteen percent of the participants lived in the metropole of Paris and had more than one dog (mean 1.72 dogs). Thirty-eight percent of the survey respondents stated that their dogs were fed exclusively with non-conventional diets, while 55% declared using conventional diets alone (not considering treats). The study canine population was for the most part neutered (63%) and purebred (68%). Amongst owners feeding conventional diets exclusively, 47% determined how much food to feed by consulting the feeding guidelines on the packaging, and only 28% said that the amount of food was prescribed by their veterinarian or veterinary nurse. Out of the participants feeding non-conventional diets, 65% declared that the information for formulating the recipes was gathered on the internet or in non-veterinary books. When compared with owners feeding exclusively conventional diets, those feeding non-conventional diets were living more frequently outside the metropole of Paris, had fewer children (0.23 ± 0.57 vs 0.37 ± 0.78; p = 0.03) and had more frequently other animals. They also dewormed less often their pets, walked their dog more each day (91 vs 78%; p p = 0.003). Conclusions This survey described differences in the habits of owners feeding dogs non-conventional diets in comparison with those feeding conventional diets. Data suggest that owners using non-conventional diets may be more attentive to the ethological needs of their dog which could be a starting point for practitioners for achieving better client-veterinarian communication.

International audience; We present herein the synthesis of biotin-functionalized polymers (BNAPols) that have been developed for the fixation of membrane proteins (MPs) onto surfaces. BNAPols were synthesized by free-radical polymerization of a tris(hydroxymethyl)acrylamidomethane (THAM)-derived amphiphilic monomer in the presence of a thiol-based transfer agent with an azido group. Then a Huisgen-cycloaddition reaction was performed with Biotin-(PEG) 8-alkyne that resulted in formation of the biotinylated polymers. The designed structure of BNAPols was confirmed by NMR spectroscopy, and a HABA/avidin assay was used for estimating the percentage of biotin grafted on the polymer end chain. The colloidal characterization of these biotin-functionalized polymers was done using both dynamic light scattering (DLS) and small angle X-ray scattering (SAXS) techniques. These BNAPols were used to stabilize a model G protein-coupled receptor (GPCR), the human Growth Hormone Secretagogue Receptor (GHSR), out of its membrane environment. Subsequent immobilization of the BNAPols:GHSR complex onto a streptavidin-coated surface allowed screening of ligands based both on their ability to bind the immobilized receptor and to trigger GHSR conformational changes using the fluorescence energy transfer (FRET)-based assay. This opens the way to the use of biotinylated NAPols to immobilize functional, unmodified, membrane proteins, providing original sensor devices for multiple applications including innovative ligand screening assays.

Radiolabeling of peptides with fluorine-18 is hurdled by their chemical sensitivity and complicated processes. Original triflyl-pyridine intermediates afforded ammonium precursors that were radiolabeled at low temperature. From that study, a generic tag has been designed to allow a simple one-step/late-stage radiolabelling of peptides. The strategy has been transposed to an automated “on-resin” radiolabelling.

Background: Current SARS-COV2 pandemic induces tensions on the health systems and ethical dilemmas. A triage tool is needed to define older patients with individual advantage to be considered for intensive care unit (ICU) transfer. Methods: This multicentre observational cohort study included patients over 60 admitted into 7 ICUs between 7th March and 7th May 2020. The primary objective was to evaluate age impact on 30-day mortality, to construct a multivariate prognostic model. This analysis explores the prediction value of geriatric parameters 1 month before ICU admission. This trial is registered with ClinicalTrials.gov, number NCT04422340. Findings: Among 290 screened patients, 231 were included in the cohort. In univariate analysis, factors associated with decreased day-30 survival were: age>75 (OR 4·82 [95%CI: 2·56-9·06]), three or more CIRS-G grade ≥2 comorbidities (OR 2·49 [95%CI: 1·36-4·56]), impaired ADL (Activities of Daily Living), (OR 4·86 [95%CI: 2·44-9·72]), impaired IADL8 (Instrumental ADL, 8 variables, OR 6·33 [95%CI: 3·31-12·10], p 75 (OR 4·64 [95%CI: 2·36-9·39], p

National audience; Development of genetic testing direct-to-consumer (DTC) for recreational purposes, although prohibited in France, is a real challenge to the current practice of gamete donation. Indeed, anonymity is a fundamental principle contributing to the ethics of donation. This principle is weakened due to the availability to the general public of these tests on the Internet. Several thousands of people are conceived by gamete donation worldwide, some of whom do not know how they were conceived. Gamete donors should be informed that their anonymity is no longer guaranteed, as they can be found by homologies of their DNA, or that of a parent or a child, potentially available in databases. Thus, adults conceived by gamete donation but not informed by their parents can discover their way of conception. Recipients of gamete donation should also be informed that their child's DNA will establish the biological discrepancy and they should be encouraged to disclose the conception to their child. Several countries now allow children conceived by donation to obtain donor's identity. In France, the Bioethics Law is currently being finalized and will now allow access to donor's identity for people conceived by gamete donation.

We report the first pretargeting in vivo study using the Strain-Promoted Sydnone–Alkyne Cycloaadition (SPSAC) reaction. The injection of a fluorine-18 labeled cyclooctyne three days after cetuximab bearing chlorosydnone moieties allowed a significant detection of the tumor by PET imaging suggesting an efficient click reaction inside the tumoral site. With a kinetic constant superior to 300 M−1 s−1, the SPSAC reaction might be an interesting tool, in addition to tetrazine-cyclooctene ligation, for in vivo chemistry.

Biologics-based radiopharmaceuticals, i.e., biological components, including peptides, proteins, polysaccharides, and nucleic acids and their combinations, are increasingly introduced in preclinical and clinical molecular imaging procedures. Synthetically prepared, extracted from natural sources, selected from biological processes, or products of cutting-edge biotechnologies, they all together represent an almost unlimited pool of radiopharmaceutical candidates. No other class of molecules offers the range of chemical diversity that biologics possess. This rich nature's toolkit uncovers a wide range of functions or dysfunctions of the human body with often incomparable potency. The downside is nevertheless that their particular chemical structures and the diversity of chemical functions they bear imply specific radiolabeling approaches. This book chapter will review the existing methods to radiolabel biologics with fluorine-18, with a particular focus on the most recent bioorthogonal conjugation approaches and strategies derived from chemical biology. An outlook to the direct in vivo labeling possibilities will also be given.

Hommage a Barbaro Teuntor Garcia Homenaje a Barbaro Teuntor Garcia Larmes, tendresse, tempete Lagrimas, ternura, tormenta Pas de mot, juste un vide No hay palabras, solo un vacio Ou un trop-plein qui s’arrete O un desbordamiento que se detiene Pour laisser place aux pleurs Para dejar lugar al llanto Quand on n’a pas assez aime Cuando uno no ha amado lo suficiente Le coup est rude, le gout, amer, El golpe es duro, el sabor, amargo, Mais le cœur palpite encore Pero el corazon aun palpita Tand...

Dans son album Entre copa y copa, canciones de cantina(2006), Lila Downs, une artiste mexico-étatsunienne de musique du monde, propose une composition intitulée « Agua de rosas », dans laquelle l’artiste prend pour thème le bain de roses que des guérisseuses zapotèques de Juchitán de Zaragoza, dans l’Etat d’Oaxaca, Mexique, lui ont donné pour qu’elle se détende et soigne ses peines. Cette chanson correspond également à un éloge funèbre adressé à Idalia Linares Sánchez, une amie personnelle. Idalia Linares représentait un membre actif de la Coalición Obrera, Campesina y Estudiantil del Istmo, une association indienne qui remporte les élections municipales de Juchitán en 1981. Un entretien avec la fille d’Idalia Linares, Marlene Linares, qui a notamment connu une expérience migratoire aux Etats-Unis, ainsi que le discours poétique de Lila Downs nous amèneront à nous interroger sur la vie et la subjectivité des femmes zapotèques, sur les continuités et les ruptures entre deux générations, sur les tensions entre l’ancien et le moderne. In her album Entre copa y copa, canciones de cantina (2006), Lila Downs, a Mexican-American artist of world music, proposes a composition entitled « Agua de rosas », a song in which the artist takes for topic the bath of roses that Zapotec healers of Juchitán de Zaragoza, in Oaxaca’s State in Mexico, gave her so that she relaxes and lightens hers sorrows. This song also corresponds to a funeral oration addressed to Idalia Linares Sánchez, a personal friend. Idalia Linares represented an active member of the Coalición Obrera, Campesina y Estudiantil del Istmo, an Indian association which wins Juchitán’s municipal elections in 1981. A discussion with Idalia Linares’ daughter, Marlene Linares, which especially knew a migratory experiment in the United States, as well as Lila Downs’ poetic discourse will lead us to wonder about the life and the subjectivity of the Zapotec women, about continuities and ruptures between two generations, about the tensions between the ancient and the modern.

International audience; Le mythe de la Llorona a inspiré de nombreuses artistes et écrivaines postmodernes hispano-américaines, qui ont converti cette Femme qui pleure mexicaine en une icône identitaire plus positive que dans la tradition orale classique. Cette approche à la fois intimiste et transnationale peut déjà s’observer dans la littérature chicana des années 80 et 90. Elle concerne désormais également des musiciennes telles que Lila Downs, chanteuse de musique du monde, ou des auteures issues d’autres groupes culturels, comme la chilienne Marcela Serrano (La Llorona, 2008).