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Your search keyword '"Möbius, Nadine"' showing total 13 results
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13 results on '"Möbius, Nadine"'

7. The zoonotic potential of Clostridium difficile from small companion animals and their owners

Author

Rabold, Denise, Espelage, Werner, Abu Sin, Muna, Eckmanns, Tim, Schneeberg, Alexander, Neubauer, Heinrich, Möbius, Nadine, Hille, Katja, Wieler, Lothar H., Seyboldt, Christian, and Lübke-Becker, Antina

Subjects
lcsh:Medicine, Pathology and Laboratory Medicine, Toxicology, Ribotyping, Feces, Zoonoses, Germany, Medicine and Health Sciences, Toxins, lcsh:Science, Child, Mammals, Aged, 80 and over, Pets and Companion Animals, Eukaryota, Pets, Middle Aged, Infectious Diseases, Veterinary Diseases, Child, Preschool, Vertebrates, Research Article, Diarrhea, Adult, Adolescent, Clostridium Difficile, Animal Types, Toxic Agents, Gastroenterology and Hepatology, Young Adult, Dogs, Signs and Symptoms, Diagnostic Medicine, Clostridium difficile infection, Animals, Humans, Aged, Bacteria, Clostridioides difficile, lcsh:R, Gut Bacteria, Organisms, Biology and Life Sciences, Infant, small companion animal, Amniotes, Cats, Clostridium Infections, lcsh:Q, Veterinary Science, CDI, Zoology
Abstract

Background: Clostridium difficile infections (CDI) in humans range from asymptomatic carriage to life-threatening intestinal disease. Findings on C. difficile in various animal species and an overlap in ribotypes (RTs) suggest potential zoonotic transmission. However, the impact of animals for human CDI remains unclear. Methods: In a large-scale survey we collected 1,447 fecal samples to determine the occurrence of C. difficile in small companion animals (dogs and cats) and their owners and to assess potential epidemiological links within the community. The Germany-wide survey was conducted from July 2012-August 2013. PCR ribotyping, Multilocus VNTR Analysis (MLVA) and PCR detection of toxin genes were used to characterize isolated C. difficile strains. A database was defined and logistic regression used to identify putative factors associated with fecal shedding of C. difficile. Results: In total, 1,418 samples met the inclusion criteria. The isolation rates for small companion animals and their owners within the community were similarly low with 3.0% (25/840) and 2.9% (17/578), respectively. PCR ribotyping revealed eight and twelve different RTs in animals and humans, respectively, whereas three RTs were isolated in both, humans and animals. RT 014/0, a well-known human hospital-associated lineage, was predominantly detected in animal samples. Moreover, the potentially highly pathogenic RTs 027 and 078 were isolated from dogs. Even though, C. difficile did not occur simultaneously in animals and humans sharing the same household. The results of the epidemiological analysis of factors associated with fecal shedding of C. difficile support the hypothesis of a zoonotic potential. Conclusions: Molecular characterization and epidemiological analysis revealed that the zoonotic risk for C. difficile associated with dogs and cats within the community is low but cannot be excluded.

Published
2018

13. Protocol for the rapid detection of the urogenital tract mollicutes and Chlamydia with concomitant LGV-(sub)typing.

Author

Möbius N, Brenneisen W, Schaeffer A, and Henrich B

Subjects
DNA Primers genetics, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, HeLa Cells, Humans, Plasmids genetics, Time Factors, Bacterial Typing Techniques methods, Chlamydia trachomatis classification, Chlamydia trachomatis genetics, Molecular Diagnostic Techniques methods, Tenericutes classification, Tenericutes genetics, Urogenital System microbiology
Abstract

Urogenital tract infections can be caused by a number of pathogens, some of which, like the obligate intracellular Chlamydia trachomatis, are difficult to culture, or the cell wall-less mollicutes, like M. hominis or Ureaplasma spp. Real-time PCR (qPCR) has become an important diagnostic tool as it enables not only the species-specific detection of the organism but also the quantification essential to define the etiological relevance of a facultative pathogenic bacterium. We developed a set of TaqMan qPCRs for the detection of the species M. genitalium and M. hominis (Mh/Mg-duplex qPCR), U. parvum and U. urealyticum (Uu/Up duplex-PCR), and C. trachomatis (CT-qPCR), and for typing of lymphogranuloma venereum-associated L-serovars of C. trachomatis (LGV-qPCR) as well as a sub-typing of L1, L2, and L3. In addition, the human gap-gene was amplified as quality control of the specimen, and a cryptic plasmid co-amplified in CT-qPCR as an inhibition control. The present protocol focuses on the step-by-step description for the establishment of these TaqMan multiplex qPCRs.

Published
2012
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