138 results on '"M Brioschi"'
Search Results
2. Multidetection scheme for transient-grating-based spectroscopy
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M. Brioschi, P. Carrara, V. Polewczyk, D. Dagur, G. Vinai, P. Parisse, S. Dal Zilio, G. Panaccione, G. Rossi, and R. Cucini
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Atomic and Molecular Physics, and Optics - Abstract
Time-resolved optical spectroscopy represents an effective non-invasive approach to investigate the interplay of different degrees of freedom, which plays a key role in the development of novel functional materials. Here, we present magneto-acoustic data on Ni thin films on SiO2 as obtained by a versatile pump–probe setup that combines transient grating spectroscopy with time-resolved magnetic polarimetry. The possibility to easily switch from a pulsed to continuous wave probe allows probing of acoustic and magnetization dynamics on a broad time scale, in both transmission and reflection geometry.
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- 2022
3. Data for proteomic analysis of Human monocyte-derived macrophages
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S. Eligini, M. Brioschi, S. Fiorelli, E. Tremoli, S. Colli, and C. Banfi
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Macrophages ,Proteomics ,Laser capture microdissection ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
This data article is referred to the research article entitled Human monocyte-derived macrophages are heterogeneous: proteomic profile of different phenotypes by Eligini et al. Eligini S., Brioschi M., Fiorelli S., Tremoli E., Banfi C., Colli S. Human monocyte-derived macrophages are heterogeneous: proteomic profile of different phenotypes. J. Proteomics 124, 2015, 112-123. Macrophages obtained in vitro from blood monocytes are largely used as surrogate model of tissue macrophages that are heterogeneous and not easy to obtain and handle. Under spontaneous differentiation in vitro, monocyte-derived macrophages (MDMs) display two dominant subsets (round and spindle) that show different transcriptional, antigenic, and functional profiles mimicking, at least in part, the heterogeneity of tissue macrophages. This article reports the nano-LC-MSE analysis of the proteome of round and spindle MDMs allowing a deeper comprehension of macrophage heterogeneity.
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- 2015
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4. All-Optical Generation and Time-Resolved Polarimetry of Magnetoacoustic Resonances via Transient Grating Spectroscopy
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P. Carrara, M. Brioschi, E. Longo, D. Dagur, V. Polewczyk, G. Vinai, R. Mantovan, M. Fanciulli, G. Rossi, G. Panaccione, and R. Cucini
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- 2022
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5. Direct anterior approach and dual mobility cup: the 'head-first' reduction technique: a technical note and early case series
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L Scaltrito, Olufemi R. Ayeni, Filippo Randelli, Pietro Randelli, M Brioschi, and Alberto Fioruzzi
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030222 orthopedics ,medicine.medical_specialty ,Head First ,business.industry ,medicine.medical_treatment ,Soft tissue ,030229 sport sciences ,Traction (orthopedics) ,Prosthesis ,Dual mobility ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Orthopedic surgery ,medicine ,Trunnion ,Orthopedics and Sports Medicine ,Anterior approach ,business - Abstract
Less invasive direct anterior approach (DAA) and dual mobility cup (DMC) are increasingly adopted in practice over the last decade. Their use aims to reduce, as much as possible, soft tissue dissection and dislocation rate. This study aims to present a novel surgical technique to reduce a DMC prosthesis during a DAA easily. A mildly modified version of the direct anterior approach is proposed. When leg lengths, stability, impingement, and tension have been checked, the trial stem is disassembled in situ, dislocated, and removed, leaving the space to exchange the trial double mobility head with the definitive one. When the definitive stem is inserted, the surgeon guides and helps the assistant to match the trunnion in the double mobility head. As soon as the components are matched, the traction is released, and the unit is impacted by an alternation of axial traction and release. Of 164 patients who underwent primary total hip arthroplasty (December 2016–May 2017) by a single surgeon, a double mobility cup through DAA and the “head-first” technique was performed in 26 patients (15.8%). The mean operative time was 130 min (85–220 min; SD 34.28). No significant complications occurred during the mean follow-up of 23.6 months. Specific difficulties can be anticipated when pairing dual mobility cup and direct anterior approach. The “head-first” technique is a useful technique in reducing the possible difficulties related to the reduction of double mobility cup through a less invasive direct anterior approach.
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- 2020
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6. Direct anterior approach and dual mobility cup: the 'head-first' reduction technique: a technical note and early case series
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F, Randelli, A, Fioruzzi, L, Scaltrito, M, Brioschi, O R, Ayeni, and P S, Randelli
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Reoperation ,Arthroplasty, Replacement, Hip ,Joint Dislocations ,Hip Dislocation ,Humans ,Hip Prosthesis ,Prosthesis Design ,Antiviral Agents ,Prosthesis Failure ,Retrospective Studies - Abstract
Less invasive direct anterior approach (DAA) and dual mobility cup (DMC) are increasingly adopted in practice over the last decade. Their use aims to reduce, as much as possible, soft tissue dissection and dislocation rate. This study aims to present a novel surgical technique to reduce a DMC prosthesis during a DAA easily.A mildly modified version of the direct anterior approach is proposed. When leg lengths, stability, impingement, and tension have been checked, the trial stem is disassembled in situ, dislocated, and removed, leaving the space to exchange the trial double mobility head with the definitive one. When the definitive stem is inserted, the surgeon guides and helps the assistant to match the trunnion in the double mobility head. As soon as the components are matched, the traction is released, and the unit is impacted by an alternation of axial traction and release.Of 164 patients who underwent primary total hip arthroplasty (December 2016-May 2017) by a single surgeon, a double mobility cup through DAA and the "head-first" technique was performed in 26 patients (15.8%). The mean operative time was 130 min (85-220 min; SD 34.28). No significant complications occurred during the mean follow-up of 23.6 months.Specific difficulties can be anticipated when pairing dual mobility cup and direct anterior approach. The "head-first" technique is a useful technique in reducing the possible difficulties related to the reduction of double mobility cup through a less invasive direct anterior approach.
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- 2019
7. Guidelines for Breast Thermography
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J. Crawford, H. Usuki, J. Head, M. Brioschi, J. Pittman, W. Amalu, B. Rind, and R.G. Schwartz
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medicine.medical_specialty ,business.industry ,Medicine ,Breast thermography ,Radiology ,business - Published
- 2015
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8. Guidelines for Neuromusculoskeletal Infrared Thermography Sympathetic Skin Response (SSR) Studies
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R.G. Schwartz, P. Getson, T. Bernton, M. Brioschi, J. Uricchio, S. Govindan, B. O’Young, and H-Y. Zhang
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business.industry ,Thermography ,Medicine ,Sympathetic skin response ,business ,Biomedical engineering - Published
- 2015
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9. Guidelines for Dental-Oral and Systemic Health Infrared Thermography
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P. Steed, K. Ammer, J. Crawford, H. Usuki, M. Brioschi, P. Getson, G. Serbu, J. Campbell, B. O’Young, and R.G. Schwartz
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medicine.medical_specialty ,business.industry ,Thermography ,Medicine ,Systemic health ,business ,Dermatology - Published
- 2015
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10. Anti-amyloid β autoantibodies in cerebral amyloid angiopathy-related inflammation: Implications for amyloid-modifying therapies
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Steven M. Greenberg, Luisa Chiapparini, Giuseppe Billo, Fabrizio Tagliavini, Jacopo C. DiFrancesco, Fabrizio Piazza, Carlo Ferrarese, Giuseppe Piscosquito, Giorgio Giaccone, M. Brioschi, Maria Rita Carriero, Antonio Colombo, Margherita Gardinetti, Mario Savoiardo, Hideya Sakaguchi, Ricardo Nitrini, Irina Raicher, and Francesca Lanzani
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Apolipoprotein E ,Pathology ,medicine.medical_specialty ,Amyloid ,business.industry ,Multiple sclerosis ,Autoantibody ,Inflammation ,medicine.disease ,Pathogenesis ,Cerebrospinal fluid ,Neurology ,mental disorders ,Immunology ,medicine ,Neurology (clinical) ,Cerebral amyloid angiopathy ,medicine.symptom ,business - Abstract
Objective Cerebral amyloid angiopathy–related inflammation (CAA-ri) is characterized by vasogenic edema and multiple cortical/subcortical microbleeds, sharing several aspects with the recently defined amyloid-related imaging abnormalities (ARIA) reported in Alzheimer's disease (AD) passive immunization therapies. Herein, we investigated the role of anti–amyloid β (Aβ) autoantibodies in the acute and remission phases of CAA-ri. Methods We used a novel ultrasensitive technique on patients from a retrospective multicenter case–control study, and evaluated the anti-Aβ autoantibody concentration in the cerebrospinal fluid (CSF) of 10 CAA-ri, 8 CAA, 14 multiple sclerosis, and 25 control subjects. Levels of soluble Aβ40, Aβ42, tau, P-181 tau, and APOE genotype were also investigated. Results During the acute phase of CAA-ri, anti-Aβ autoantibodies were specifically increased and directly correlated with Aβ mobilization, together with augmented tau and P-181 tau. Following clinical and radiological remission, autoantibodies progressively returned to control levels, and both soluble Aβ and axonal degeneration markers decreased in parallel. Interpretation Our data support the hypothesis that the pathogenesis of CAA-ri may be mediated by a selective autoimmune reaction against cerebrovascular Aβ, directly related to autoantibody concentration and soluble Aβ. The CSF dosage of anti-Aβ autoantibodies with the technique here described can thus be proposed as a valid alternative tool for the diagnosis of CAA-ri. Moreover, given the similarities between ARIA developing spontaneously and those observed during immunization trials, anti-Aβ autoantibodies can be considered as novel potential biomarkers in future amyloid-modifying therapies for the treatment of AD and CAA. Ann Neurol 2013;73:449–458
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- 2013
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11. ISNI 2012 Abstracts
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Fabrizio Piazza, Margherita Gardinetti, Luisa Chiapparini, Jacopo C. DiFrancesco, Fabrizio Tagliavini, Maria Rita Carriero, Mario Savoiardo, M. Brioschi, Giuseppe Piscosquito, Irina Raicher, Giuseppe Billo, Francesca Lanzani, Antonio Colombo, Hideya Sakaguchi, Carlo Ferrarese, Giorgio Giaccone, and Steven M. Greenberg
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business.industry ,Immunology ,Autoantibody ,Inflammation ,Disease ,medicine.disease ,Article ,Pathogenesis ,Immune system ,Neurology ,Immunology and Allergy ,Medicine ,Neurology (clinical) ,Cerebral amyloid angiopathy ,medicine.symptom ,business ,Beta (finance) - Published
- 2012
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12. TISSUE FACTOR INDUCTION BY PROTEASE-ACTIVATED RECEPTOR-1 REQUIRES INTACT CAVEOLIN-ENRICHED MEMBRANE MICRODOMAINS IN HUMAN ENDOTHELIAL CELLS
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C. Banfi, M. Brioschi, S. Barcella, A. Pignieri, E. Tremoli, A. Parolari, P. Biglioli, and L. Mussoni
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Hematology - Published
- 2007
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13. Proceedings of the 17th Congress of the Polish Association of Thermology, Zakopane, March15th-17th, 2013
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Ammer, Kurt, Ring, Francis, J. B. Mercer, L. De Weerdt, J. E. Løkebø, A. Seixas, A. Silva, J. G. Mendes, R. Vardasca, P. Murawski, A. Jung, B. Kalicki, B. Rustecki, A. Cholewka, A. Stanek, S. Kwiatek, A. Sieron, Z. Drzazga, S. Naseer, K. G. Keresztes, T. J. Coats, N. A. Urakowa, B. Wiecek, M. Soroko, R. Henklewski, H. Filipowski, E. Jodkowska, J. Adamczyk, D. Boguszewski, M. Siewierski, D. Bialoszewski, A. Slupik, A. Mosiolek, M. Brioschi, M. Teixeira, L. Yeng, G. Franco, J. Araujo, M. Lima, A. Marcondes, P. Freitas, J. Badaro, A. A. Kasatkin, A. I. Urakow, M. Strakowska, W. Wittchen, S. Marzec, A. Rustecka, J. Zuber, R. Strakowski, and G. De May
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- 2013
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14. Primary intracranial hypotension: Pathogenetic and neuroradiological considerations
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C Reverdito, Enrico Ferrante, Riva M, A M Gerini, A. M. Brioschi, and A Guccione
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Adult ,Male ,medicine.medical_specialty ,Neurology ,Intracranial Pressure ,Dermatology ,Hypotension, Orthostatic ,medicine ,Humans ,Spontaneous Intracranial Hypotension ,Rare syndrome ,Intracranial Hypotension ,Neuroradiology ,medicine.diagnostic_test ,business.industry ,General Neuroscience ,Brain ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,Spinal cord ,Magnetic Resonance Imaging ,Psychiatry and Mental health ,medicine.anatomical_structure ,Anesthesia ,Female ,Neurology (clinical) ,Neurosurgery ,Tomography, X-Ray Computed ,business - Abstract
Primary or spontaneous intracranial hypotension (SIH) is a rare syndrome which causes postural headache associated with spinal fluid hypotension. We report three cases of SIH, characterised on magnetic resonance imaging (MRI) by pachymeningeal enhancement not only at cerebral level, but also in the cervical spinal cord, which subsequently resolved completely and spontaneously. We discuss the possible pathogenetic mechanisms of the dural alterations and underline the radiological aspects.
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- 1995
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15. Anti-Aβ autoantibodies in the CSF of a patient with CAA-related inflammation: a case report
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Jacopo C. DiFrancesco, M. Brioschi, Mario Savoiardo, C. Ruffmann, E. Saracchi, N. A. Curtò, Gloria Galimberti, G. Costantino, L Brighina, Paolo Remida, Elisa Conti, Fabrizio Tagliavini, Carlo Ferrarese, L. Marzorati, DI FRANCESCO, J, Brioschi, M, Brighina, L, Ruffmann, C, Saracchi, E, Costantino, G, Galimberti, G, Conti, E, Curtò, N, Marzorati, L, Remida, P, Tagliavini, F, Savoiardo, M, and Ferrarese, C
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Amyloid beta-Peptide ,Inflammation ,Nerve Fibers, Myelinated ,Immunoglobulin G ,White matter ,Cerebrospinal fluid ,Peptide Fragment ,Internal medicine ,Medicine ,Humans ,Aged ,Autoantibodies ,Aged, 80 and over ,Amyloid beta-Peptides ,medicine.diagnostic_test ,biology ,business.industry ,Lumbar puncture ,Autoantibody ,Middle Aged ,medicine.disease ,Autoantibodie ,Hyperintensity ,Peptide Fragments ,Cerebral Amyloid Angiopathy ,medicine.anatomical_structure ,Endocrinology ,Biological Marker ,biology.protein ,Neurology (clinical) ,Cerebral amyloid angiopathy ,medicine.symptom ,business ,Biomarkers ,Human - Abstract
A 68-year-old man presented with a 4-month history of progressive memory loss and mood disorders. Neurologic examination revealed severe impairment of attention and verbal skills, without motor and sensory deficits. His medical history included mild arterial hypertension, idiopathic partial epilepsy, and obsessive compulsive disorder. Brain MRI showed the presence of bilateral, asymmetric, swollen white matter lesions in the cerebral hemispheres, hyperintense in T2-weighted images, that partially involved the left frontal cortex (figure). On diffusion-weighted sequences, the white matter abnormalities were consistent with vasogenic edema. No pathologic contrast enhancement was present. Figure MRI of cerebral amyloid angiopathy–related inflammation (CAA-ri) and levels of anti-Aβ 1-40 and 1-42 autoantibodies in the CSF Axial fluid-attenuated inversion recovery brain MRI shows bilateral hyperintense lesions of the subcortical white matter (A), which are reduced after 20 days of steroid treatment (B). Axial T2*-weighted gradient-echo MRI (C) obtained 33 days later shows further reduction of white matter lesions and multiple, scattered, hypointense cortical lesions due to microhemorrhages (arrows). (D) Reiber's graph. X- and y-axes show, respectively, albumin (QAlb) and immunoglobulin G quotient (QIgG), obtained by the ratio between the level of the protein in the CSF from the first lumbar puncture and in the plasma. The QAlb indicates the permeability of the blood–brain barrier (BBB) to water-soluble molecules. The QIgG (total IgG including specific anti-Aβ autoantibodies) plotted into the graph discriminates between intrathecal production of IgG and …
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- 2011
16. Cerebellar hematoma in a carrier of the A3243G MELAS mutation
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Barbara Frigeni, M. Brioschi, L. Marzorati, Enrico Saracchi, Carlo Ferrarese, Lorenzo Fumagalli, L Brighina, Lucio Tremolizzo, Jacopo C. DiFrancesco, N. A. Curtò, Maria Luisa Piatti, G Costantino, Saracchi, E, Tremolizzo, L, DI FRANCESCO, J, Brighina, L, Costantino, G, Frigeni, B, Brioschi, M, Piatti, M, Fumagalli, L, Marzorati, L, Curtò, N, and Ferrarese, C
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Adult ,Male ,medicine.medical_specialty ,Encephalopathy ,Neurological examination ,Dermatology ,MELAS syndrome ,Angiopathy ,Hematoma ,Mitochondrial myopathy ,Internal medicine ,medicine ,MELAS Syndrome ,Stroke ,Intracerebral hemorrhage ,medicine.diagnostic_test ,business.industry ,Electromyography ,Electroencephalography ,General Medicine ,Cerebellar Disease ,medicine.disease ,Surgery ,Psychiatry and Mental health ,Mutation ,Cardiology ,Neurology (clinical) ,business ,Human - Abstract
Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is often related to the A3243G mutation of the mitochondrial DNA, accounting for about 80% of typical cases, with a relevant phenotypic variability. Hemorrhagic strokes are quite unusual in these patients. A case of recurrent brain hematomas has been reported in a MELAS patient carrying the G13513A mutation, with clinical onset at age 31 [1]. Another fatal case of intracerebral hemorrhage was reported in a MERRF/MELAS overlap syndrome due to a T8356C mutation [2]. Finally, a putaminal hemorrhagic stroke was described in a MERRF patient carrying the A8344G mutation, with clinical onset at age 26 [3]. Here, we report the case of a 37-year-old male arriving to our attention because of a two-week history of headache associated with nausea, vomiting, and dizziness. A brain MRI scan showed a left hemispheric cerebellar hemorrhage at a stage compatible with the onset of the symptoms. The patient neither had history of arterial hypertension, any hemorrhagic risk-factor, diabetes, nor was taking any medication. A few years before, he was found to be asymptomatic carrier of the A3243G mitochondrial DNA mutation (heteroplasmy confirmed on a blood sample), since a MELAS diagnosis was made on his younger brother expressing a typical phenotype. On current examination, our patient presented slight left ataxic hemiparesis and hypoesthesia. A cerebral angiography failed to show any vascular abnormality. Since our patient was carrier of a MELAS mutation, we decided to assess its putative clinical burden. An EEG showed generalized electrogenetic dysfunction with a single generalized epileptic spike discharge; EMG suggested slight myopathic signs; mild perceptive bilateral deafness was also present. A venous lactic acid test with compression of the right arm was significant (baseline values 2.9 mmol/L; increase up to 7.5 mmol/L after 3 min; NV 0.44–2.22 mmol/L). No hearth conduction defects were present. A week later, on a control CT scan, progressive blood reabsorption was noted and basal ganglia calcifications were not observed; the headache had improved and the neurological examination was normal. Iizuka et al. [4] demonstrated cortical laminar necrosis and focal HMPAO SPET hyperperfusion during subacute stages of stroke-like episodes; moreover, they reported several cases of microhemorrhages and at least one case of intracortical gyral hemorrhage, suggesting that stroke-like episodes are characterized by increased capillary permeability and focal hyperemia [5]. Consistently, vasogenic edema has been reported during stroke-like episodes [6]. Anyhow, in our case, the aspect of the lesion does not suggest the hemorrhagic transformation of a stroke-like episode; this also considering that the hematoma was confined in a specific vascular territory, which is not a characteristic of stroke-like episodes [4]. Conversely, we cannot exclude a hemorrhagic coincidental event in a patient carrying the A3243G mutation with a subclinical MELAS phenotype. However, mitochondrial angiopathy in E. Saracchi L. Tremolizzo J. C. DiFrancesco L. Brighina G. Costantino B. Frigeni M. Brioschi M. L. Piatti L. Fumagalli L. Marzorati N. A. Curto C. Ferrarese Department of Neurology, S. Gerardo Hospital, Monza, Italy
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- 2011
17. Chapter 11 - Solid lipid nanoparticles for brain tumors therapy: State of the art and novel challenges
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Andrea M, Brioschi, Sara, Calderoni, Gian Paolo, Zara, Lorenzo, Priano, Maria Rosa, Gasco, and Alessandro, Mauro
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Disease Models, Animal ,Neuropharmacology ,Blood-Brain Barrier ,Brain Neoplasms ,Animals ,Humans ,Nanoparticles ,Nanotechnology ,Antineoplastic Agents ,Neurochemistry ,Medical Oncology ,Lipids ,Rats - Abstract
Malignant gliomas, despite aggressive multimodal therapies and adequate supportive care, still maintain poor prognosis. Solid lipid nanoparticles (SLN) are colloidal carriers that could be regarded as a highly flexible platform for brain tumor imaging and therapeutical purposes. In this chapter we will first describe brain tumors characteristics and conventional therapeutical approaches. In the subsequent sections, we will analyze SLN properties, effectiveness, and future perspectives in both imaging and targeted treatment of malignant gliomas.
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- 2010
18. Development of Services for Mobile Information Systems
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Danilo Ardagna, N. Simeoni, Carlo Batini, Marco Comuzzi, Marco Comerio, Simone Grega, Chiara Francalanci, Luciano Baresi, Andrea Maurino, Cinzia Cappiello, Stefano Modafferi, F. De Paoli, M. Brioschi, Pernici, B, Simeoni, N, Modafferi, S, Maurino, A, Grega, S, Francalanci, C, DE PAOLI, F, Comuzzi, M, Comerio, M, Cappiello, C, Brioschi, M, Batini, C, Baresi, L, and Ardagna, D
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Development (topology) ,Multimedia ,Computer science ,Mobile information systems ,development, services, mobile information systems ,Calculus ,computer.software_genre ,computer ,ING-INF/05 - SISTEMI DI ELABORAZIONE DELLE INFORMAZIONI - Published
- 2006
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19. Reference Architecture and Framework
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Barbara Pernici, P. Losi, Carla Simone, Paolo Cappellari, Andrea Maurino, M. Riva, C. Franza, M Adorni, Tiziana Catarci, Claudia Raibulet, Pierluigi Plebani, E. Mussi, C. Pandolfo, Francesco Tisato, Daniela Micucci, A. Limonta, Stefano Modafferi, Davide Ragazzi, R. Torlone, M. Melideo, Antonio Zilli, Gianluca Solazzo, Cinzia Cappiello, A. A. Bianchi, Gianluca Lorenzo, G. Giunta, Francesca Arcelli, V. De Antonellis, L. Negri, Giuseppe Vizzari, M. Brioschi, N. Simeoni, A. Caforio, Andrea Calì, Stefania Bandini, Carlo Batini, Angelo Corallo, Luciano Baresi, Devis Bianchini, Pernici, B, Adorni, M, ARCELLI FONTANA, F, Bandini, S, Baresi, L, Batini, C, Bianchi, A, Bianchini, D, Brioschi, M, Caforio, A, Cal`i, A, Cappellari, P, Cappiello, C, Catarci, T, Corallo, A, De Antonellis, V, Franza, C, Giunta, G, Limonta, A, Lorenzo, G, Losi, P, Maurino, A, Melideo, M, Micucci, D, Modafferi, S, Mussi, E, Negri, L, Pandolfo, C, Plebani, P, Ragazzi, D, Raibulet, C, Riva, M, Simeoni, N, Simone, C, Vizzari, G, Solazzo, G, Tisato, F, Torlone, R, Zilli, A, Adorni, M., Arcelli, F., Bandini, Tullio, Baresi, L., Batini, C., Bianchi, A., Brioschi, M., Caforio, A., Calì, A., Cappellari, P., Cappiello, C., Catarci, T., Corallo, A., De Antonellis, V., Franza, Cosimo, Giunta, G., Limonta, A., Lorenzo, G., Losi, P., Maurino, A., Melideo, M., and Micucci, D.
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software architecture ,business.industry ,Multichannel information systems ,media_common.quotation_subject ,INF/01 - INFORMATICA ,Art ,reference architecture ,architectural model ,adaptive information systems ,Smart card ,Reference architecture ,business ,Humanities ,media_common - Abstract
The goal of the MAIS system is to provide support for flexible and adaptive execution of applications in a distributed, multichannel, mobile information system. In such a system, a fundamental requirement is an ability to describe the continuously evolving execution environment and user characteristics. Service requests are therefore satisfied by considering both the request itself and its provisioning environment. The first part of this chapter presents the general architecture of the MAIS system. The MAIS architecture allows us to define a set of “pluggable” modules which can be composed to provide adaptivity at different levels in the MAIS system. The main architectural components are introduced in Sect. 2.2; more details of the components are provided in the rest of the book. The MAIS reference framework, illustrated in the second part of this chapter, provides the essential basis for all of the adaptive mechanisms that are illustrated in the book. The MAIS reference framework defines a common understanding of the elements of a mobile information system that are used to enable communication among the various modules of a MAIS system during information exchange and service provisioning. The reference framework is composed of a set of models: the functional model, the architectural model, and the context model. These are described in the second part of this chapter.
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- 2006
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20. Value of [11C]choline-positron emission tomography for re-staging prostate cancer: a comparison with [18F]fluorodeoxyglucose-positron emission tomography
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F. Fazio, Maria Picchio, Mario Matarrese, A. Del Maschio, P. Rigatti, M. Brioschi, F. De Cobelli, C. Landoni, Francesco Rocco, Deliu Victor Matei, Sandro Sironi, Luigi Gianolli, Cristina Messa, Picchio, M, Messa, C, Landoni, C, Gianolli, L, Sironi, S, Brioschi, M, Matarrese, M, Matei, Dv, DE COBELLI, Francesco, DEL MASCHIO, Alessandro, Rocco, F, Rigatti, Patrizio, and Fazio, F.
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Male ,Urology ,medicine.medical_treatment ,Sensitivity and Specificity ,Choline ,Prostate cancer ,chemistry.chemical_compound ,Positron ,Prostate ,Fluorodeoxyglucose F18 ,medicine ,Biomarkers, Tumor ,Humans ,Carbon Radioisotopes ,Aged ,Neoplasm Staging ,Fluorodeoxyglucose ,Aged, 80 and over ,Prostatectomy ,medicine.diagnostic_test ,business.industry ,Prostatic Neoplasms ,Middle Aged ,Prostate-Specific Antigen ,medicine.disease ,Prostate-specific antigen ,medicine.anatomical_structure ,chemistry ,Positron emission tomography ,Nuclear medicine ,business ,medicine.drug ,Tomography, Emission-Computed - Abstract
PURPOSE:We compared [11C]choline-positron emission tomography (PET) with [18F]fluorodeoxyglucose-PET for re-staging prostate cancer in a group of 100 patients.MATERIALS AND METHODS:A total of 100 consecutive patients referred for whole body [18F]fluorodeoxyglucose-PET for clinical prostate re-staging after radical treatment for prostate cancer were retrospectively included in the study. Mean prostate specific antigen (PSA) was 6.57 ng./ml. In all cases [11C]choline-PET was also performed. PET studies were done with a multiring device 5 minutes after intravenous injection of approximately 370 MBq. [11C]choline and 60 minutes after injection of approximately 370 MBq. [18F]fluorodeoxyglucose. PET findings were compared with those obtained with different conventional imaging and with PSA assessed at the time of PET and 1 year later.RESULTS:Areas of abnormal focal increases were noted in 47% of patients on [11C]choline-PET and in 27% on [18F]fluorodeoxyglucose-PET. Of the 100 patients 49 had positive conventional imaging findings. All except 14 [11C]choline-PET findings were concordant with conventional imaging, including 6 negative and 8 positive conventional imaging results. All except 1 [11C]choline-PET negative cases also had negative conventional imaging after 1 year. PSA at 1 year remained stable or decreased in 80% and 62% of [11C]choline-PET negative and positive cases, respectively.CONCLUSIONS:[11C]choline-PET seems to be useful for re-staging prostatectomy cases with increasing serum PSA levels. It is superior to [18F]fluorodeoxyglucose-PET and complementary to conventional imaging but with the advantage of staging disease at a single step
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- 2003
21. Immunological and endocrinological abnormalities in paraneoplastic disorders with involvement of the autonomic nervous system
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A. M. Brioschi, M. F. Donato, Marazzi R, Riva M, and Enrico Ferrante
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Male ,medicine.medical_specialty ,Neurology ,Lung Neoplasms ,Paraneoplastic Syndromes ,Encephalopathy ,Dermatology ,Gastroenterology ,Internal medicine ,medicine ,Humans ,Lung cancer ,Aged ,Leukemia ,business.industry ,General Neuroscience ,Limbic encephalitis ,Cancer ,Polyradiculoneuropathy ,General Medicine ,Middle Aged ,medicine.disease ,Psychiatry and Mental health ,Autonomic nervous system ,Autonomic Nervous System Diseases ,Immunology ,Colonic Neoplasms ,Neurology (clinical) ,business ,Complication - Abstract
We report a series of four patients in whom the onset of systemic cancer was heralded by dysautonomic symptoms and a neurological non-metastatic complication mediated by immunological and endocrine factors. The series includes: a patient with acute leukaemia and autonomic sensory-motor polyradiculoneuropathy, a patient affected by colon carcinoma and autonomic neuropathy and limbic encephalitis, a patient with lung cancer and autonomic neuropathy and hypercalcaemic encephalopathy, a patient with small cell lung cancer associated with autonomic neuropathy in Lambert-Eaton Myasthenic Syndrome (LEMS) and syndrome of inappropriate ADH secretion (SIADH). We underline the prognostic importance and discuss the possible etiopathogenetic role of autonomic dysfunction, which is frequently associated with paraneoplastic neurologic syndromes of autoimmune and/or dysendocrine origin.
- Published
- 1997
22. Autologous anti-Aß antibodies in CAA-ri: New biomarker for detection of amyloid-related imaging abnormalities (ARIA) during Aß-disease modifying therapies for AD
- Author
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Steven M. Greenberg, Ricardo Nitrini, G Giaccone, Luisa Chiapparini, Jacopo C. DiFrancesco, Margherita Gardinetti, Irina Raicher, F Lanzani, Fabrizio Piazza, M Carriero, Antonio Colombo, Giuseppe Piscosquito, Carlo Ferrarese, Fabrizio Tagliavini, Hideya Sakaguchi, G Billo, Mario Savoiardo, and M. Brioschi
- Subjects
Pathology ,medicine.medical_specialty ,Amyloid ,biology ,business.industry ,Disease ,Neurology ,Immunology ,biology.protein ,Biomarker (medicine) ,Medicine ,Neurology (clinical) ,Antibody ,business - Published
- 2013
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- View/download PDF
23. [Central pontine myelinolysis as potential complication of cerebellar astrocytoma: report of a case]
- Author
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M, Riva, A M, Brioschi, E, Ferrante, and R, Marazzi
- Subjects
Male ,Myelinolysis, Central Pontine ,Humans ,Astrocytoma ,Middle Aged ,Cerebellar Neoplasms - Abstract
Central pontine myelinolysis (CPM), a rare condition first observed by Adams et al. in 1959 in a group of malnourished chronic alcoholic subjects, has subsequently been seen in patients treated with thiazide diuretics, patients hyperhydrated postoperatively, and in other clinical situations. it is characterized by quadriplegia and pseudobulbar palsy which sometimes evolves into a locked-in syndrome. The rapid correction of severe hyponatremia (12 mmol/L/24 h) seems to be the causal factor, with consequent osmotic edema in the richly vascularized white matter of the pons as the proposed pathogenetic mechanism. We describe the case of a chronic psychotic man with nutritional disorders and inappropriate water intake who came to our attention for a clinical picture of CPM. Neuroradiological findings and postmortem studies revealed a slow-growing cerebellar astrocytoma in addition to the typical features of CPM. We discuss the hypothesis that damage to the nervous pathways and centers involved in water and electrolyte regulation could be the causal factor of CPM pathogenesis in this case.
- Published
- 1996
24. Chronic Paroxysmal Hemicrania in early Childhood: Case Report
- Author
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H Larocca Santos, P Afonso Cunali, D Benzecry de Almeida, M Brioschi, and M. Prandini
- Subjects
Pediatrics ,medicine.medical_specialty ,business.industry ,MEDLINE ,General Medicine ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Chronic disease ,Chronic paroxysmal hemicrania ,medicine ,030212 general & internal medicine ,Neurology (clinical) ,Early childhood ,business ,030217 neurology & neurosurgery - Published
- 2004
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- View/download PDF
25. Neuroradiological improvement after one year of therapy in a case of DHPR deficiency
- Author
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M. Giovannini, R. Valsasina, Elisabetta Riva, L. Saleri, M. Brioschi, and Giacomo Biasucci
- Subjects
Pediatrics ,medicine.medical_specialty ,business.industry ,Medicine ,DHPR deficiency ,business - Published
- 1990
- Full Text
- View/download PDF
26. Sur les équations différentielles linéaires du second ordre
- Author
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M. Hermite and M. Brioschi
- Subjects
Pure mathematics ,Applied Mathematics ,Mathematics - Published
- 1880
- Full Text
- View/download PDF
27. Cerebral amyloid angiopathy-related inflammation: An emerging disease
- Author
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Floriano Girotti, J. C. Di Francesco, Alessandra Erbetta, M. Brioschi, Mario Savoiardo, Nicola Ticozzi, Andrea Falini, L. Brighina, Stefano Messina, G. Storchi, Vincenzo Silani, Carlo Ferrarese, Savoiardo, Mario, Erbetta, A., Di Francesco, J. C., Brioschi, M., Silani, V., Falini, A., Storchi, G., Brighina, L., Ferrarese, C., Ticozzi, N., Messina, S., Girotti, F., Savoiardo, M, Erbetta, A, DI FRANCESCO, J, Brioschi, M, Silani, V, Falini, A, Storchi, G, Brighina, L, Ferrarese, C, Ticozzi, N, Messina, S, and Girotti, F
- Subjects
Apolipoprotein E ,Pathology ,medicine.medical_specialty ,Radiology, Nuclear Medicine and Imaging ,Amyloid ,Inflammation ,Disease ,White matter ,Microhemorrhage ,Cortex (anatomy) ,Edema ,mental disorders ,medicine ,Radiology, Nuclear Medicine and imaging ,Cerebral amyloid angiopathy ,business.industry ,APOe, cerebral amyloid angiopathy-related inflammation , MRI ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Neurology (clinical) ,medicine.symptom ,business ,MRI - Abstract
Three elderly patients with, respectively: mild cognitive impairment, severe and progressive neurologic involvement, and focal neurologic deficit, were observed. MRI showed multiple areas of white matter edema, at times partially involving the cortex, in the first two patients, and a single area in the third. Treatment with steroids determined the disappearance of the lesions and clinical amelioration. The key to the diagnosis of cerebral amyloid angiopathy-related inflammation (CAA-ri) was the demonstration, with appropriate MRI sequences, of microbleeds consistent with cerebral amyloid angiopathy (CAA). This diagnosis was supported by genetic analysis of APOE with demonstration of ε4/ε4 genotype, found in about 80% of CAA patients who develop inflammatory changes. In the appropriate clinical setting, MRI demonstration of microbleeds supported by results of genetic analysis of APOE may strongly support the diagnosis of CAA-ri thus avoiding cerebral biopsy.
28. Logica dei relativi, semiotica e fenomenologia. Per un Peirce 'Non-Standard'
- Author
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PAOLUCCI, CLAUDIO, U. Eco, R. Calcaterra, G. Maddalena, M. Brioschi, M. Stango, C. Paolucci, E. Fadda, P. Facchi, S. Petrilli, F. Silvestri, A. Martone, C. Caputo, A. Ponzio, F. Cimatti, V. Pisanty, D. Goldoni, G. Proni, S. Zingale, M. Bonfantini, R. Fabbrichesi, M. A. Bonfantini, R. Fabbrichesi, S. Zingale, and Paolucci, Claudio
- Subjects
FENOMENOLOGIA ,PEIRCE, LOGICA DEI RELATIVI, SEMIOTICA ,COGNIZIONE - Abstract
In questo lavoro proveremo a proporre un’interpretazione che si muove in una diversa direzione rispetto a quella ‘standard’ che si è data del pensiero di Peirce nella tradizione semiotica. Vorremmo provare a fornirne una lettura fondata su tre oggetti teorici chiave e sulle loro reciproche interconnessioni: la logica delle relazioni, la semiotica e la fenomenologia che, unite insieme, ci sembra possano fornire un’immagine adeguata della teoria peirceana della cognizione.
- Published
- 2015
29. Targeting protein aggregation using a cocoa-bean shell extract to reduce α-synuclein toxicity in models of Parkinson's disease.
- Author
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Tripodi F, Lambiase A, Moukham H, Spandri G, Brioschi M, Falletta E, D'Urzo A, Vai M, Abbiati F, Pagliari S, Salvo A, Spano M, Campone L, Labra M, and Coccetti P
- Abstract
Neurodegenerative diseases are among the major challenges in modern medicine, due to the progressive aging of the world population. Among these, Parkinson's disease (PD) affects 10 million people worldwide and is associated with the aggregation of the presynaptic protein α-synuclein (α-syn). Here we use two different PD models, yeast cells and neuroblastoma cells overexpressing α-syn, to investigate the protective effect of an extract from the cocoa shell, which is a by-product of the roasting process of cocoa beans. The LC-ESI-qTOF-MS and NMR analyses allow the identification of amino acids (including the essential ones), organic acids, lactate and glycerol, confirming also the presence of the two methylxanthines, namely caffeine and theobromine. The present study demonstrates that the supplementation with the cocoa bean shell extract (CBSE) strongly improves the longevity of yeast cells expressing α-syn, reducing the level of reactive oxygen species, activating autophagy and reducing the intracellular protein aggresomes. These anti-aggregation properties are confirmed also in neuroblastoma cells, where CBSE treatment leads to activation of AMPK kinase and to a significant reduction of toxic α-syn oligomers. Results obtained by surface plasmon resonance (SPR) assay highlights that CBSE binds α-syn protein in a concentration-dependent manner, supporting its inhibitory role on the amyloid aggregation of α-syn. These findings suggest that the supplementation with CBSE in the form of nutraceuticals may represent a promising way to prevent neurodegenerative diseases associated with α-syn aggregation., Competing Interests: The authors have declared no conflict of interests., (© 2024 The Authors. Published by Elsevier B.V.)
- Published
- 2024
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30. Excitation and detection of coherent nanoscale spin waves via extreme ultraviolet transient gratings.
- Author
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Miedaner PR, Berndt N, Deschamps J, Urazhdin S, Khatu N, Fainozzi D, Brioschi M, Carrara P, Cucini R, Rossi G, Wittrock S, Ksenzov D, Mincigrucci R, Bencivenga F, Foglia L, Paltanin E, Bonetti S, Engel D, Schick D, Gutt C, Comin R, Nelson KA, and Maznev AA
- Abstract
The advent of free electron lasers has opened the opportunity to explore interactions between extreme ultraviolet (EUV) photons and collective excitations in solids. While EUV transient grating spectroscopy, a noncollinear four-wave mixing technique, has already been applied to probe coherent phonons, the potential of EUV radiation for studying nanoscale spin waves has not been harnessed. Here we report EUV transient grating experiments with coherent magnons in Fe/Gd ferrimagnetic multilayers. Magnons with tens of nanometers wavelengths are excited by a pair of femtosecond EUV pulses and detected via diffraction of a probe pulse tuned to an absorption edge of Gd. The results unlock the potential of nonlinear EUV spectroscopy for studying magnons and provide a tool for exploring spin waves in a wave vector range not accessible by established inelastic scattering techniques.
- Published
- 2024
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- View/download PDF
31. Mass spectrometry for the study of adipocyte cell secretome in cardiovascular diseases.
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Gianazza E, Brioschi M, Eligini S, and Banfi C
- Subjects
- Humans, Animals, Adipose Tissue metabolism, Proteomics methods, Cardiovascular Diseases metabolism, Adipocytes metabolism, Mass Spectrometry methods, Secretome metabolism
- Abstract
Adipose tissue is classically considered the primary site of lipid storage, but in recent years has garnered appreciation for its broad role as an endocrine organ, capable of remotely signaling to other tissues to alter their metabolic program. The adipose tissue is now recognized as a crucial regulator of cardiovascular health, mediated by the secretion of several bioactive products, with a wide range of endocrine and paracrine effects on the cardiovascular system. Thanks to the development and improvement of high-throughput mass spectrometry, the size and components of the human secretome have been characterized. In this review, we summarized the recent advances in mass spectrometry-based studies of the cell and tissue secretome for the understanding of adipose tissue biology, which may help to decipher the complex molecular mechanisms controlling the crosstalk between the adipose tissue and the cardiovascular system, and their possible clinical translation., (© 2022 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd.)
- Published
- 2024
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- View/download PDF
32. Coherent and Dissipative Coupling in a Magnetomechanical System.
- Author
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Carrara P, Brioschi M, Silvani R, Adeyeye AO, Panaccione G, Gubbiotti G, Rossi G, and Cucini R
- Abstract
Hybrid elastic and spin waves hold promises for energy-efficient and versatile generation and detection of magnetic signals, with potentially long coherence times. Here we report on the combined elastic and magnetic dynamics in a one-dimensional magnetomechanical crystal composed of an array of magnetic nanostripes. Phononic and magnonic modes are impulsively excited by an optical ultrafast trigger and their decay is monitored by time-resolved magneto-optical Kerr effect. Complementary Brillouin light scattering measurements and micromagnetic simulations concur in a unified picture, in which the strength and degree of mixing of coherent and dissipative coupling of the quasiparticles are determined quantitatively.
- Published
- 2024
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- View/download PDF
33. Vigna unguiculata L. Walp. Leaves as a Source of Phytochemicals of Dietary Interest: Optimization of Ultrasound-Assisted Extraction and Assessment of Traditional Consumer Habits.
- Author
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Pioltelli E, Sartirana C, Copetta A, Brioschi M, Labra M, and Guzzetti L
- Subjects
- Tandem Mass Spectrometry, Phytochemicals, Ethanol chemistry, Solvents, Habits, Vigna chemistry
- Abstract
Vigna unguiculata L. Walp. is an African crop spread worldwide mainly for pulses production. Despite being a neglected and under-utilized food, cowpea leaves are a rich source of phytochemicals and micronutrients. The aim of the work is to characterize the phytochemical composition of cowpea leaves by an optimized ultrasound-assisted extraction (USAE) and to compare raw and boiled leaves. A three-level factorial design (Box-Behnken) was employed for the optimization of the USAE considering three different parameters (% ethanol, drug-to-solvent ratio, and number of cycles). The optimized extracts were characterized by LC/MS/MS. Finally, leaves were boiled at 100 °C for 30 min to simulate traditional cooking procedures and compared to raw leaves. The best extraction condition was EtOH/H
2 O 1 : 2 v/v, drug to solvent ratio 1 : 47 w/v, and 3 extraction cycles. The phytochemicals identified mainly belong to the family of phenolic acids, flavonoids, terpenoids, and alkaloids. Boiled leaves revealed a significant loss of most phytochemicals and a net decrease of their antioxidant activity compared to the raw ones. The results highlight the potential nutraceutical value of cowpea leaves whilst the impoverishment triggered by traditional consumer habits pushes the need to evaluate alternative cooking procedures helpful in the maintenance of their phytochemical properties., (© 2023 The Authors. Chemistry & Biodiversity published by Wiley-VHCA AG, Zurich, Switzerland.)- Published
- 2023
- Full Text
- View/download PDF
34. Antarctic Soil Metabolomics: A Pilot Study.
- Author
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Ciaramelli C, Palmioli A, Brioschi M, Viglio S, D'Amato M, Iadarola P, Tosi S, Zucconi L, and Airoldi C
- Subjects
- Antarctic Regions, Pilot Projects, Chromatography, Liquid, Metabolomics methods, Soil chemistry, Tandem Mass Spectrometry
- Abstract
In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in the McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions can survive and reproduce. Metabolic analyses can shed light on the survival mechanisms of Antarctic soil communities from both coastal sites, under different plant coverage stages, and inner sites where slow-growing or dormant microorganisms, low water availability, salt accumulation, and a limited number of primary producers make metabolomic profiling difficult. Here, we report, for the first time, an efficient protocol for the extraction and the metabolic profiling of Antarctic soils based on the combination of NMR spectroscopy and mass spectrometry (MS). This approach was set up on samples harvested along different localities of Victoria Land, in continental Antarctica, devoid of or covered by differently developed biological crusts. NMR allowed for the identification of thirty metabolites (mainly sugars, amino acids, and organic acids) and the quantification of just over twenty of them. UPLC-MS analysis identified more than twenty other metabolites, in particular flavonoids, medium- and long-chain fatty acids, benzoic acid derivatives, anthracenes, and quinones. Our results highlighted the complementarity of the two analytical techniques. Moreover, we demonstrated that their combined use represents the "gold standard" for the qualitative and quantitative analysis of little-explored samples, such as those collected from Antarctic soils.
- Published
- 2023
- Full Text
- View/download PDF
35. Novel insights about albumin in cardiovascular diseases: Focus on heart failure.
- Author
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Zoanni B, Brioschi M, Mallia A, Gianazza E, Eligini S, Carini M, Aldini G, and Banfi C
- Subjects
- Humans, Proteome metabolism, Proteomics, Albumins, Cardiovascular Diseases, Heart Failure
- Abstract
The Human Plasma Proteome has always been the most investigated compartment in proteomics-based biomarker discovery, and is considered the largest and deepest version of the human proteome, reflecting the state of the body in health and disease. Even if efforts have been always dedicated to the refinement of proteomic approaches to investigate more deeply the plasma proteome, it should not be forgotten that also highly abundant plasma proteins, like human serum albumin (HSA), often neglected in these studies, might provide fundamental physiological functions in plasma, and should be better considered. This review summarizes the important roles of HSA in the context of cardiovascular diseases (CVD), and in particular in heart failure. Notwithstanding much attention has been historically directed toward the association of HSA levels and CVD risk, the advances in the field of mass spectrometry research allow also a better characterization of the effects of oxidative modifications that could alter not only the structure but also the function of HSA., (© 2021 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
36. Proteomic studies on apoB-containing lipoprotein in cardiovascular research: A comprehensive review.
- Author
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Gianazza E, Zoanni B, Mallia A, Brioschi M, Colombo GI, and Banfi C
- Subjects
- Humans, Proteomics, Lipoproteins, Mass Spectrometry, Apolipoproteins B, Cardiovascular Diseases
- Abstract
The complexity of cardiovascular diseases (CVDs), which remains the leading cause of death worldwide, makes the current clinical pathway for cardiovascular risk assessment unsatisfactory, as there remains a substantial unexplained residual risk. Simultaneous assessment of a large number of plasma proteins may be a promising tool to further refine risk assessment, and lipoprotein-associated proteins have the potential to fill this gap. Technical advances now allow for high-throughput proteomic analysis in a reproducible and cost-effective manner. Proteomics has great potential to identify and quantify hundreds of candidate marker proteins in a sample and allows the translation from isolated lipoproteins to whole plasma, thus providing an individual multiplexed proteomic fingerprint. This narrative review describes the pathophysiological roles of atherogenic apoB-containing lipoproteins and the recent advances in their mass spectrometry-based proteomic characterization and quantitation for better refinement of CVD risk assessment., (© 2021 The Authors. Mass Spectrometry Reviews published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
37. Impact of Sacubitril/Valsartan on Circulating microRNA in Patients with Heart Failure.
- Author
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Brioschi M, D'Alessandra Y, Mapelli M, Mattavelli I, Salvioni E, Eligini S, Mallia A, Ricci V, Gianazza E, Ghilardi S, Agostoni P, and Banfi C
- Abstract
Sacubitril/Valsartan, used for the treatment of heart failure (HF), is a combination of two drugs, an angiotensin receptor inhibitor, and a neprilysin inhibitor, which activates vasoactive peptides. Even though its beneficial effects on cardiac functions have been demonstrated, the mechanisms underpinning these effects remain poorly understood. To achieve more mechanistic insights, we analyzed the profiles of circulating miRNAs in plasma from patients with stable HF with reduced ejection function (HFrEF) and treated with Sacubitril/Valsartan for six months. miRNAs are short (22-24 nt) non-coding RNAs, which are not only emerging as sensitive and stable biomarkers for various diseases but also participate in the regulation of several biological processes. We found that in patients with high levels of miRNAs, specifically miR-29b-3p, miR-221-3p, and miR-503-5p, Sacubitril/Valsartan significantly reduced their levels at follow-up. We also found a significant negative correlation of miR-29b-3p, miR-221-3p, and miR-503-5p with VO
2 at peak exercise, whose levels decrease with HF severity. Furthermore, from a functional point of view, miR-29b-3p, miR-221-3p, and miR-503-5p all target Phosphoinositide-3-Kinase Regulatory Subunit 1, which encodes regulatory subunit 1 of phosphoinositide-3-kinase. Our findings support that an additional mechanism through which Sacubitril/Valsartan exerts its functions is the modulation of miRNAs with potentially relevant roles in HFrEF pathophysiology.- Published
- 2023
- Full Text
- View/download PDF
38. Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis.
- Author
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Banfi C, Mallia A, Ghilardi S, Brioschi M, Gianazza E, Eligini S, Sahlén P, and Baetta R
- Abstract
The process of adipogenesis involves the differentiation of preadipocytes into mature adipocytes. Excessive adipogenesis promotes obesity, a condition that increasingly threatens global health and contributes to the rapid rise of obesity-related diseases. We have recently shown that prenylcysteine oxidase 1 (PCYOX1) is a regulator of atherosclerosis-disease mechanisms, which acts through mechanisms not exclusively related to its pro-oxidant activity. To address the role of PCYOX1 in the adipogenic process, we extended our previous observations confirming that Pcyox1
-/- /Apoe-/- mice fed a high-fat diet for 8 or 12 weeks showed significantly lower body weight, when compared to Pcyox1+/+ /Apoe-/- mice, due to an evident reduction in visceral adipose content. We herein assessed the role of PCYOX1 in adipogenesis. Here, we found that PCYOX1 is expressed in adipose tissue, and, independently from its pro-oxidant enzymatic activity, is critical for adipogenesis. Pcyox1 gene silencing completely prevented the differentiation of 3T3-L1 preadipocytes, by acting as an upstream regulator of several key players, such as FABP4, PPARγ, C/EBPα. Proteomic analysis, performed by quantitative label-free mass spectrometry, further strengthened the role of PCYOX1 in adipogenesis by expanding the list of its downstream targets. Finally, the absence of Pcyox1 reduces the inflammatory markers in adipose tissue. These findings render PCYOX1 a novel adipogenic factor with possible pathophysiological or therapeutic potential.- Published
- 2023
- Full Text
- View/download PDF
39. Pharmacometabolomics for the Study of Lipid-Lowering Therapies: Opportunities and Challenges.
- Author
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Gianazza E, Brioschi M, Iezzi A, Paglia G, and Banfi C
- Subjects
- Humans, Hypolipidemic Agents, Precision Medicine, Lipids, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Drug-Related Side Effects and Adverse Reactions drug therapy
- Abstract
Lipid-lowering therapies are widely used to prevent the development of atherosclerotic cardiovascular disease (ASCVD) and related mortality worldwide. "Omics" technologies have been successfully applied in recent decades to investigate the mechanisms of action of these drugs, their pleiotropic effects, and their side effects, aiming to identify novel targets for future personalized medicine with an improvement of the efficacy and safety associated with the treatment. Pharmacometabolomics is a branch of metabolomics that is focused on the study of drug effects on metabolic pathways that are implicated in the variation of response to the treatment considering also the influences from a specific disease, environment, and concomitant pharmacological therapies. In this review, we summarized the most significant metabolomic studies on the effects of lipid-lowering therapies, including the most commonly used statins and fibrates to novel drugs or nutraceutical approaches. The integration of pharmacometabolomics data with the information obtained from the other "omics" approaches could help in the comprehension of the biological mechanisms underlying the use of lipid-lowering drugs in view of defining a precision medicine to improve the efficacy and reduce the side effects associated with the treatment.
- Published
- 2023
- Full Text
- View/download PDF
40. Multidetection scheme for transient-grating-based spectroscopy.
- Author
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Brioschi M, Carrara P, Polewczyk V, Dagur D, Vinai G, Parisse P, Dal Zilio S, Panaccione G, Rossi G, and Cucini R
- Abstract
Time-resolved optical spectroscopy represents an effective non-invasive approach to investigate the interplay of different degrees of freedom, which plays a key role in the development of novel functional materials. Here, we present magneto-acoustic data on Ni thin films on SiO
2 as obtained by a versatile pump-probe setup that combines transient grating spectroscopy with time-resolved magnetic polarimetry. The possibility to easily switch from a pulsed to continuous wave probe allows probing of acoustic and magnetization dynamics on a broad time scale, in both transmission and reflection geometry.- Published
- 2023
- Full Text
- View/download PDF
41. The Effects of Silencing PTX3 on the Proteome of Human Endothelial Cells.
- Author
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Banfi C, Brioschi M, Vicentini LM, and Cattaneo MG
- Subjects
- Humans, C-Reactive Protein metabolism, Proteomics, Human Umbilical Vein Endothelial Cells metabolism, Neovascularization, Pathologic, Inflammation genetics, Inflammation metabolism, Serum Amyloid P-Component metabolism, Proteome
- Abstract
The human long pentraxin PTX3 has complex regulatory roles at the crossroad of innate immunity, inflammation, and tissue repair. PTX3 can be produced by various cell types, including vascular endothelial cells (ECs), in response to pro-inflammatory cytokines or bacterial molecules. PTX3 has also been involved in the regulation of cardiovascular biology, even if ambiguous results have been so far provided in both preclinical and clinical research. In this study, we compared the proteomic profiles of human ECs (human umbilical vein ECs, HUVECs), focusing on differentially expressed proteins between the control and PTX3-silenced ECs. We identified 19 proteins that were more abundant in the proteome of control ECs and 23 proteins that were more expressed in PTX3-silenced cells. Among the latter, proteins with multifunctional roles in angiogenesis, oxidative stress, and inflammation were found, and were further validated by assessing their mRNAs with RT-qPCR. Nevertheless, the knock down of PTX3 did not affect in vitro angiogenesis. On the contrary, the lack of the protein induced an increase in pro-inflammatory markers and a shift to the more oxidative profile of PTX3-deficient ECs. Altogether, our results support the idea of a protective function for PTX3 in the control of endothelial homeostasis, and more generally, in cardiovascular biology.
- Published
- 2022
- Full Text
- View/download PDF
42. N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin.
- Author
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Altomare AA, Brioschi M, Eligini S, Bonomi A, Zoanni B, Iezzi A, Jemos C, Porro B, D'Alessandra Y, Guarino A, Omodeo Salè E, Aldini G, Agostoni P, and Banfi C
- Abstract
Human serum albumin (HSA) represents the most abundant plasma protein, with relevant antioxidant activity due to the presence of the sulfhydryl group on cysteine at position 34 (Cys34), the latter being one of the major target sites for redox-dependent modifications leading to the formation of mixed disulfide linkages with low molecular weight thiols. Thiolated forms of HSA (Thio-HSA) may be useful as markers of an unbalanced redox state and as a potential therapeutic target. Indeed, we have previously reported that albumin Cys34 can be regenerated in vitro by N -Acetylcysteine (NAC) through a thiol-disulfide breaking mechanism, with a full recovery of the HSA antioxidant and antiplatelet activities. With this case study, we aimed to assess the ability of NAC to regenerate native mercaptoalbumin (HSA-SH) and the plasma antioxidant capacity in subjects with redox unbalance, after oral and intravenous administration. A placebo-controlled crossover study, single-blinded, was performed on six hypertensive subjects, randomized into two groups, on a one-to-one basis with NAC (600 mg/die) or a placebo, orally and intravenously administered. Albumin isoforms, HSA-SH, Thio-HSA, and glutathione levels were evaluated by means of mass spectrometry. The plasma antioxidant activity was assessed by a fluorimetric assay. NAC, orally administered, significantly decreased the Thio-HSA levels in comparison with the pre-treatment conditions (T0), reaching the maximal effect after 60 min (-24.7 ± 8%). The Thio-HSA reduction was accompanied by a concomitant increase in the native HSA-SH levels (+6.4 ± 2%). After intravenous administration of NAC, a significant decrease of the Thio-HSA with respect to the pre-treatment conditions (T0) was observed, with a maximal effect after 30 min (-68.9 ± 10.6%) and remaining significant even after 6 h. Conversely, no effect on the albumin isoforms was detected with either the orally or the intravenously administered placebo treatments. Furthermore, the total antioxidant activity of the plasma significantly increased after NAC infusion with respect to the placebo ( p = 0.0089). Interestingly, we did not observe any difference in terms of total glutathione corrected for hemoglobin, ruling out any effect of NAC on the intracellular glutathione and supporting its role as a disulfide-breaking agent. This case study confirms the in vitro experiments and demonstrates for the first time that NAC is able to regenerate mercaptoalbumin in vivo, allowing us to hypothesize that the recovery of Cys34 content can modulate in vivo oxidative stress and, hopefully, have an effect in oxidative-based diseases.
- Published
- 2022
- Full Text
- View/download PDF
43. The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus.
- Author
-
Piarulli F, Banfi C, Brioschi M, Altomare A, Ragazzi E, Cosma C, Sartore G, and Lapolla A
- Abstract
Human serum albumin (HSA) has an important antioxidant activity due to the presence of the reduced cysteine at position 34, which represents the most abundant free thiol in the plasma. In oxidative-based diseases, HSA undergoes S-thiolation (THIO-HSA) with changes in the antioxidant function of albumin that could contribute to the progression of the disease. The aim of this study was to verify, for the first time, the different burdens of THIO-HSA, glycated HSA (GLY-HSA), and advanced glycation end products (AGE) accumulation both in type 2 diabetes mellitus (T2DM) patients and in non-diabetic patients, with or without coronary heart disease (CHD). In this study, we assessed the presence of modified forms of HSA, THIO-HSA, and GLY-HSA by means of mass spectrometry in 33 patients with both T2DM and CHD, in 31 patients with T2DM and without CHD, in 30 patients without diabetes with a history of CHD, and 27 subjects without diabetes and CHD. All the patients' anthropometric and clinical data were recorded including age, sex, duration of diabetes, body mass index (BMI), blood pressure, and history of CHD defined with anamnestic data. Metabolic parameters, such as fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), lipids, pentosidine, AGE, receptor for advanced glycation end-products (RAGE) and its soluble form (sRAGE), were measured. AGE and pentosidine are significantly higher in T2DM patients with and without CHD with respect to non-diabetic patients with CHD and control subjects. RAGE levels are significantly higher in T2DM patients with respect to non-diabetic patients, and among T2DM patients, the group with CHD showed significantly higher RAGE levels than those without CHD (217 ± 171 pg/mL and 140 ± 61 pg/mL, respectively). Albumin isoforms discriminate between non-diabetic patients with CHD and T2DM patients with and without CHD and control subjects, with GLY-HSA levels higher in T2DM with and without CHD, and THIO-HSA higher in CHD patients without T2DM. Finally, we demonstrated that the oxidized forms of HSA can increase the expression of the inflammatory cytokine Tumor Necrosis Factor-alpha (TNFα) in monocytic cells. In patients with CHD, GLY-HSA and THIO-HSA have a different prevalent distribution, the first one prevailing in patients with T2DM and the second one in patients without T2DM. These findings suggest that albumin quality and homeostasis balance between glyco-oxidation and thiolation might have an impact on the antioxidant defense system in cardiovascular diseases.
- Published
- 2022
- Full Text
- View/download PDF
44. Mercaptoalbumin Is Associated with Graft Patency in Patients Undergoing Coronary Artery Bypass Grafting.
- Author
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Brioschi M, Gianazza E, Andreini D, Mushtaq S, Cavallotti L, Veglia F, Tedesco CC, Colombo GI, Pepi M, Polvani G, Tremoli E, Parolari A, and Banfi C
- Abstract
Coronary artery bypass graft (CABG) surgery still represents the gold standard for patients with complex multivessel coronary artery disease. However, graft occlusion still occurs in a significant proportion of CABG conduits, and oxidative stress is currently considered to be a potential contributor. Human serum albumin (HSA) represents the main antioxidant in plasma through its reduced amino acid Cys34, which can efficiently scavenge several oxidants. In a nested case-control study including 36 patients with occluded grafts and 38 age- and sex-matched patients without occlusion, we assessed the levels of the native mercaptoalbumin (HSA-SH) and oxidized thiolated form of albumin (Thio-HSA) in relation with graft occlusion within 5 years after CABG. We found that the plasma level of preoperative HSA-SH was significantly lower in patients with occluded graft at 5 years follow-up than in patients with graft patency. Furthermore, low HSA-SH remained independently associated with graft occlusion even after adjusting for preoperative D-dimer, a well-known marker of activated coagulation recently found to be associated with graft occlusion. In conclusion, the preoperative level of HSA-SH is independently associated with graft occlusion in CABG and represents a measurable and potentially druggable predictor.
- Published
- 2022
- Full Text
- View/download PDF
45. Prenylcysteine Oxidase 1 (PCYOX1), a New Player in Thrombosis.
- Author
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Banfi C, Amadio P, Zarà M, Brioschi M, Sandrini L, and Barbieri SS
- Subjects
- Animals, Blood Platelets metabolism, Cysteine analogs & derivatives, Fibrinogen metabolism, Mice, Oxidoreductases metabolism, Platelet Activation, Platelet Aggregation, Platelet Glycoprotein GPIIb-IIIa Complex metabolism, Carbon-Sulfur Lyases metabolism, Endothelial Cells metabolism, Thrombosis metabolism
- Abstract
Prenylcysteine Oxidase 1 (PCYOX1) is an enzyme involved in the degradation of prenylated proteins. It is expressed in different tissues including vascular and blood cells. We recently showed that the secretome from Pcyox1 -silenced cells reduced platelet adhesion both to fibrinogen and endothelial cells, suggesting a potential contribution of PCYOX1 into thrombus formation. Here, we show that in vivo thrombus formation after FeCl
3 injury of the carotid artery was delayed in Pcyox1-/- mice, which were also protected from collagen/epinephrine induced thromboembolism. The Pcyox1-/- mice displayed normal blood cells count, vascular procoagulant activity and plasma fibrinogen levels. Deletion of Pcyox1 reduced the platelet/leukocyte aggregates in whole blood, as well as the platelet aggregation, the alpha granules release, and the αIIb β3 integrin activation in platelet-rich plasma, in response to adenosine diphosphate (ADP) or thrombin receptor agonist peptide (TRAP). Washed platelets from the Pcyox1-/- and WT animals showed similar phosphorylation pathway activation, adhesion ability and aggregation. The presence of Pcyox1-/- plasma impaired agonist-induced WT platelet aggregation. Our findings show that the absence of PCYOX1 results in platelet hypo-reactivity and impaired arterial thrombosis, and indicates that PCYOX1 could be a novel target for antithrombotic drugs.- Published
- 2022
- Full Text
- View/download PDF
46. Correction to: Floating hip in polytraumatized patients: complications, mechanism of injury, and surgical strategy.
- Author
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Brioschi M, Randelli F, Capitani P, and Capitani D
- Published
- 2022
- Full Text
- View/download PDF
47. Floating hip in polytraumatized patients: complications, mechanism of injury, and surgical strategy.
- Author
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Brioschi M, Randelli F, Capitani P, and Capitani D
- Subjects
- Acetabulum injuries, Acetabulum surgery, Fracture Fixation, Internal adverse effects, Fracture Fixation, Internal methods, Humans, Retrospective Studies, Treatment Outcome, Femoral Fractures surgery, Pelvic Bones injuries
- Abstract
Purpose: Floating hip fractures are severe and rare injuries with high complication rate and lack of standard management. The purpose of this retrospective study was to review the outcomes of a case series of polytraumatized patients with floating hip injuries, in order to describe the relationship between complication rate and floating hip injury type, the pathomechanism of injury, and the surgical treatment strategy., Methods: Forty-five patients with floating hip injuries were analyzed. Complication rate, mechanism of injury, and surgical strategy (damage control orthopedics, stages of internal fixation) were recorded. Fractures were classified using the Mueller system for floating hips and AO/OTA system for the other fractures., Results: Of the 45 patients with floating hip injuries, 23 (51.1%) developed complications. The results revealed that the complication rate was associated with the instability and type of the pelvic or acetabular fracture (61-C AO/OTA pelvic fracture [73.3%; p = 0.04], 62-B AO/OTA acetabular fracture [88.8%; p = 0.03]). Vertical shear (VS) pelvic fractures were related to middle-distal femoral fractures and lateral compression/anteroposterior compression (LC/APC) pelvic lesions were associated to proximal femoral fractures (p = 0.012)., Conclusions: Complications were associated with the severity and instability of the pelvic and acetabular type of fractures. The pathomechanism causing Mueller type B and C floating hip injuries was illustrated. A two-stage "femur first" surgical approach was the preferred one for definite internal fixation of fractures., (© 2021. The Author(s) under exclusive licence to SICOT aisbl.)
- Published
- 2022
- Full Text
- View/download PDF
48. A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors.
- Author
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Fierle JK, Abram-Saliba J, Atsaves V, Brioschi M, de Tiani M, Reichenbach P, Irving M, Coukos G, and Dunn SM
- Subjects
- Cell Line, Tumor, Gene Library, HEK293 Cells, Healthy Volunteers, Humans, Immunotherapy, Adoptive methods, Neoplasms immunology, Neoplasms pathology, Primary Cell Culture, Receptors, Chimeric Antigen genetics, Receptors, Chimeric Antigen immunology, Single-Chain Antibodies immunology, Single-Chain Antibodies metabolism, T-Lymphocytes, Cytotoxic metabolism, T-Lymphocytes, Cytotoxic transplantation, Neoplasms therapy, Receptors, Chimeric Antigen isolation & purification, T-Lymphocytes, Cytotoxic immunology
- Abstract
Anti-tumor therapies that seek to exploit and redirect the cytotoxic killing and effector potential of autologous or syngeneic T cells have shown extraordinary promise and efficacy in certain clinical settings. Such cells, when engineered to express synthetic chimeric antigen receptors (CARs) acquire novel targeting and activation properties which are governed and orchestrated by, typically, antibody fragments specific for a tumor antigen of interest. However, it is becoming increasingly apparent that not all antibodies are equal in this regard, with a growing appreciation that 'optimal' CAR performance requires a consideration of multiple structural and contextual parameters. Thus, antibodies raised by classical approaches and intended for other applications often perform poorly or not at all when repurposed as CARs. With this in mind, we have explored the potential of an in vitro phenotypic CAR library discovery approach that tightly associates antibody-driven bridging of tumor and effector T cells with an informative and functionally relevant CAR activation reporter signal. Critically, we demonstrate the utility of this enrichment methodology for 'real world' de novo discovery by isolating several novel anti-mesothelin CAR-active scFv candidates., (© 2022. The Author(s).)
- Published
- 2022
- Full Text
- View/download PDF
49. Erratum: Altomare et al. In-Depth AGE and ALE Profiling of Human Albumin in Heart Failure: Ex Vivo Studies. Antioxidants 2021, 10 , 358.
- Author
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Altomare A, Baron G, Balbinot M, Martínez Fernández A, Pedretti A, Zoanni B, Brioschi M, Agostoni P, Carini M, Banfi C, and Aldini G
- Abstract
The authors of this paper [...].
- Published
- 2021
- Full Text
- View/download PDF
50. Multiomic Approaches to Uncover the Complexities of Dystrophin-Associated Cardiomyopathy.
- Author
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Gowran A, Brioschi M, Rovina D, Chiesa M, Piacentini L, Mallia S, Banfi C, Pompilio G, and Santoro R
- Subjects
- Animals, Cardiomyopathies etiology, Cardiomyopathies metabolism, Humans, Cardiomyopathies pathology, Computational Biology methods, Dystrophin deficiency, Genome, Proteome analysis, Transcriptome
- Abstract
Despite major progress in treating skeletal muscle disease associated with dystrophinopathies, cardiomyopathy is emerging as a major cause of death in people carrying dystrophin gene mutations that remain without a targeted cure even with new treatment directions and advances in modelling abilities. The reasons for the stunted progress in ameliorating dystrophin-associated cardiomyopathy (DAC) can be explained by the difficulties in detecting pathophysiological mechanisms which can also be efficiently targeted within the heart in the widest patient population. New perspectives are clearly required to effectively address the unanswered questions concerning the identification of authentic and effectual readouts of DAC occurrence and severity. A potential way forward to achieve further therapy breakthroughs lies in combining multiomic analysis with advanced preclinical precision models. This review presents the fundamental discoveries made using relevant models of DAC and how omics approaches have been incorporated to date.
- Published
- 2021
- Full Text
- View/download PDF
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