1. Glutathione S-transferase isoenzymes in decidua and placenta of preeclamptic pregnancies
- Author
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Hans M.W.M. Merkus, Eric A.P. Steegers, Petra L.M. Zusterzeel, Maarten F. C. M. Knapen, Wilbert H.M. Peters, and M. A. H. De Bruyn
- Subjects
Adult ,HELLP Syndrome ,medicine.medical_specialty ,Voeding en afweer ,Placenta ,Karakterisering van factoren betrokken bij de etiologie van ernstige pre-eclampsie en het HELLP (Hemolysis, Elevated Liver enzymes, Low Platelets) syndroom tijdens de zwangerschap ,Isozyme ,Preeclampsia ,Pathogenesis ,chemistry.chemical_compound ,Pre-Eclampsia ,Pregnancy ,Internal medicine ,Decidua ,medicine ,Humans ,Characterisation of factors involved in the etiology of severe preeclampsia and the HELLP (Hemolysis, Elevated Liver enzymes, Low Platelets) syndrome during pregnancy ,reproductive and urinary physiology ,Glutathione Transferase ,biology ,Obstetrics and Gynecology ,Glutathione ,medicine.disease ,Enzyme assay ,Isoenzymes ,Nutrition and inflammation ,Glutathione S-transferase ,Endocrinology ,medicine.anatomical_structure ,chemistry ,embryonic structures ,biology.protein ,Female ,Lipid Peroxidation - Abstract
Objective: To investigate a possible involvement of glutathione S-transferases, major detoxificating enzymes, in the pathophysiology of preeclampsia. Methods: Levels of glutathione S-transferase isoforms and enzyme activity were assessed in placental and decidual tissues in 22 preeclamptic and 21 normotensive women. Measured values were analyzed statistically using the Mann-Whitney U test for comparison between groups, and the signed-rank test for comparison within groups. Results: Glutathione S-transferase pi is the main glutathione S-transferase isoform in normal placental and decidual tissue. Lower median placental and decidual glutathione S-transferase pi levels were found in preeclamptic women compared with controls: 1268 (range: 524–3925) and 2185 (range: 503–6578), P = .05, for placenta; 1543 (range: 681–2967) and 2169 (range: 893–3929), P = .02, for decidua. The total amount of glutathione S-transferases in control and preeclamptic pregnancies was higher in decidua than in placenta. Conclusion: Reduced levels of glutathione S-transferase class pi in preeclampsia might indicate a decreased capacity of the glutathione/glutathione S-transferase detoxification system. A higher total amount of glutathione S-transferases in decidual tissue might point to a more pronounced protective role of decidua compared with placenta.
- Published
- 1999
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