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STUDY QUESTION What are the pregnancy and live birth rates for ovarian tissue transplantation and which factors are associated with the success rate? SUMMARY ANSWER Pregnancy and live birth rates per transplanted woman are 32.7% and 26.5% and success rate is associated with female age and first versus repeated transplantation. WHAT IS KNOWN ALREADY Live birth rates after ovarian tissue transplantations have been reported to be between around 24% and 41% per patient. Success rates seem to be negatively associated with increasing female age at the time of tissue cryopreservation and with pelvic radiation. Success rates are apparently not reduced after overnight transportation of ovarian tissue before freezing. STUDY DESIGN, SIZE, DURATION Registry analysis of 244 transplantations in 196 women, performed by 26 FertiPROTEKT network centres from 2007 to 2019 with follow-up till December 2020. PARTICIPANTS/MATERIALS, SETTING, METHODS Orthotopic ovarian tissue transplantations were performed in 196 women, 191 with previous malignant and 5 with previous non-malignant diseases. Size of transplanting centres varied between 1 and 100 transplantations per centre (median: 2). Factors possibly associated with success rate such as female age, first and repeated transplantation, experience of the transplanting centre and overnight transportation of the ovarian tissue before freezing were analysed. MAIN RESULTS AND THE ROLE OF CHANCE Average age of all 196 transplanted women was 31.3 years (SD 5.2; range 17–44) at the time of cryopreservation of tissue and 35.9 years (SD 4.8; range 23–47) at the time of transplantation. Pregnancy rate was 30.6% (95% CI, 24.2–37.6%) per first transplantation and 32.7% (95% CI, 26.1–39.7%) per patient. Pregnancy rate was higher after first transplantation (30.6% (95% CI, 24.2–37.6%)) compared to second and subsequent transplantations (11.8% (95% CI, 3.3–27.5%)). Live birth rate per first transplantation was 25.0% (95% CI, 19.1–31.7%) and per patient 26.5% (95% CI, 20.5–33.3%). Success rate decreased with increasing age at the time of ovarian tissue freezing. Live birth rate was 28.2% (95% CI, 20.9–36.3%) in women 35 years. Pregnancy rates after first transplantation were higher in centres who had performed ≥10 transplantations (35.1%) compared to centres with LIMITATIONS, REASONS FOR CAUTION The data were drawn from a registry analysis. Data such as ovarian reserve and premature ovarian insufficiency were not available for all women. Data might be influenced by different follow-up policies of the centres. WIDER IMPLICATIONS OF THE FINDINGS The study reveals the high potential of ovarian tissue freezing and transplantation, but only if freezing is performed in younger women. The study suggests focus should be placed on the first and not on repeated transplantations. It also opens the discussion of whether transplantation should rather be performed by experienced centres. STUDY FUNDING/COMPETING INTEREST(S) No funding. No competing interests. TRIAL REGISTRATION NUMBER N/A.

Sexual minority women (SMW) report high rates of hazardous drinking (HD), treatment utilization that is not commensurate with need, and low perceived alcohol use severity. This study examined SMW's drinking problem concerns by sexual identity and other demographic characteristics, and the strength of associations between endorsement of unique HD indicators and drinking problem concerns. Data were from a supplemental sample of SMW added in Wave 3 of the longitudinal, community-based Chicago Health and Life Experiences of Women (CHLEW) study. HD was measured using a validated 13-item HD Index (HDI). Multivariable logistic regression models examined independent associations between past-five-year drinking problem concerns and each HD indicator. Twelve HD indicators were significantly associated with past-five-year drinking problem concerns, adjusting for age and sexual identity. Adjusted odds ratios varied from 2.44 for driving drunk to 15.52 for drinking first thing in the morning. After adjusting for number of HD indicators endorsed, associations were no longer significant, indicating that number of endorsed indicators was a more important predictor of drinking problem concerns than were individual HD indicators. Early intervention and harm reduction strategies could support SMW in addressing salient aspects of HD before progression to alcohol use disorder.

PURPOSE: Research on opioid misuse, opioid use disorder (OUD), and overdose (i.e., opioid outcomes) among lesbian, gay, bisexual, transgender, queer, and other populations within the LGBTQ umbrella (LGBTQ+) remains sparse. The purpose of this scoping review was to characterize the state of the research on opioid outcomes among LGBTQ+ populations, and identify gaps in the extant literature and areas for future research. METHODS: We conducted a scoping review of peer-reviewed, English language articles published between 2011 and 2020 that examined opioid outcomes among LGBTQ+ populations in the CINAHL, Embase, PubMed, and PsycINFO databases. We extracted data from articles that focused on opioid outcomes within their specific aims or purpose. We include a general summary for articles that secondarily described opioid outcomes among LGBTQ+ populations. RESULTS: Of 113 published studies that examined opioid outcomes among LGBTQ+ populations, 10% (n = 11) were specifically designed to focus on this topic. Across studies, bisexual populations, particularly women, were at highest risk for opioid misuse and OUD. Few studies examined opioid outcomes by more than one dimension of sexual orientation (n = 3, 27%), race and/or ethnicity (n = 3, 27%), or age (n = 5, 45%). Only two included transgender or gender diverse samples; only one explicitly measured gender identity. CONCLUSIONS: Future research is needed to understand the impact of the opioid epidemic on LGBTQ+ people, particularly transgender and other gender diverse individuals, and the intersectional role of race, ethnicity, and age in opioid disparities among LGBTQ+ individuals. Additional research could contribute to the development of much-needed affirming OUD treatment and other services for LGBTQ+ people.

In clinical practice, micronized progesterone (MP) is frequently recommended to treat signs and symptoms of skin and hair aging in menopausal women. The aim of this comprehensive review was to evaluate whether topically or systemically applied MP may effectively prevent or slow down signs of skin and hair aging. Three out of six identified studies reported an impact of MP on skin aging markers in menopausal women. Of these, two studies reported a benefit: one for topically applied MP, another for systemically applied combined menopausal hormone therapy (MHT) comprising MP as progestogen for endometrial protection. Tolerability and safety of MP were good. However, there was no study investigating the impact of MP on menopausal scalp hair. In conclusion, delay of skin aging comprises lifestyle adjustment, antioxidants, and several esthetic procedures. In menopausal women, MHT displays beneficial effects on skin aging. There is poor quality but promising scientific evidence for MP displaying anti-aging skin effects in menopausal women. However, good quality studies are needed.

Study question What are the associations between cycle regime and early pregnancy complications after frozen-thawed embryo transfers and what are the respective life birth rates per pregnancy? Summary answer Hormone replacement cycles are associated with doubled odd ratios of bleedings and miscarriages What is known already So far, there are only few studies which analysed both, the associations between cycle regime and early pregnancy complications as well as life birth rates (LBR) per pregnancy after frozen-thawed embryo transfer (FET). In 2017, a Cochrane analysis did not find sufficient evidence to support the use of a specific cycle regimen in preference to another since there were only four direct comparisons. In 2021, a network meta-analysis including 26 RCTs and 113 cohort studies revealed lowest LBR in hormone replacement cycles compared with other endometrial preparation protocols but without stating the reasons for the lowest LBR. Study design, size, duration Retrospective cohort study analyzing a total of 7342 pregnancies after FET that were registered in the Swiss IVF Registry from 2014 to 2019. Participants/materials, setting, methods Women were divided into three groups according to the different cycle regimens: Natural Cycles (NC-FET, n = 998), low-dose Stimulation Cycles (SC-FET, n = 984) and Hormone Replacement Cycles (HRC-FET, n = 5360) leading to pregnancy. Outcomes included early pregnancy complications such as bleedings, miscarriages and ectopic pregnancies, deliveries (including intrauterine deaths) and LBR. Incidences were compared using Fisher exact and Chi-square tests. Multivariate mixed model analysis with pregnancy complications as outcome was performed. Main results and the role of chance The incidences of bleeding in first trimester (NC: 3.5%, SC: 4.3%, HRC: 8.4%; p Multivariate analysis revealed doubled odds ratio of bleedings in first trimester (aOR 1.92; 95% CI 1.30-2.81; p Limitations, reasons for caution The data were drawn from a registry analysis with limited information on patients’ characteristics. Data such as BMI were not available. As the study is based on a register analysis, some errors in data collection cannot be excluded. Wider implications of the findings This is the latest large European register study evaluating early pregnancy complications and LBR per pregnancy after FET by cycle regime. Miscarriage rate was highest in HRC-FET which can be translated into lower LBR. Thus, HRC-FET should be avoided if medically possible, to achieve better pregnancy outcomes. Trial registration number Non required

Study question What are the associations between cycle regime and maternal and neonatal outcomes after frozen-thawed embryo transfer leading to singleton or twin life birth? Summary answer Hormone replacement cycles are associated with higher and natural or low-dose stimulation with lower risks for bleeding, hypertension and preeclampsia in singleton and twin pregnancies. What is known already So far, there is no consensus on optimal cycle regimen for endometrial preparation: Frozen-thawed embryo transfers can be either performed in Natural Cycles (NC-FET), low-dose Stimulation Cycles (SC-FET) or Hormone Replacement Cycles (HRC-FET). In previous studies, HRC-FET was shown to be associated with an increase of serious maternal and neonatal complications, i.e. preeclampsia (doubled to tripled risk), postpartum hemorrhage (doubled risk), placenta accrete (sixfold risk), post-term birth (sixfold risk) and Caesarean section (doubled risk) compared to NC-FET or SC-FET. These results indicate that risks are increased in cycles in which the development of follicles and luteal bodies is medically inhibited. Study design, size, duration Retrospective cohort study analyzing a total of 4636 singletons and also 544 twins born after FET that were registered in the Swiss IVF Registry from 2014 to 2019. Participants/materials, setting, methods Women were divided into three groups according to the cycle regimes: NC-FET (n = 776), SC-FET (n = 758) and HRC-FET (n = 3646) leading to life birth. Maternal outcomes included pregnancy complications such as bleedings, isolated hypertension (>140/90 mmHg) and preeclampsia. Neonatal outcomes comprised gestational age and weight, mode of delivery and neonatal status. Incidences were compared using Fisher exact or Chi-square tests. Multivariate mixed model analysis was performed for singleton and twin pregnancies. Main results and the role of chance In singleton pregnancies, the incidences of bleeding in first trimester (NC: 2.8%, SC: 2.6%, HRC: 7.0%; p In twin pregnancies, the incidences of preeclampsia were significantly higher in HRC-FET (NC: 2.7%, SC: 1.0%, HRC: 7.2%, p = 0.026). There were no differences in delivery mode between cycle regime. Multivariate analysis revealed numerically higher odds ratio of preeclampsia in HRC-FET vs. NC-FET (aOR 2.57; 95% CI 0.55 - 12.07; p = 0.232) and in HRC-FET vs. SC-FET (aOR 4.07; 95% CI 0.47 - 34.91; p = 0.2) but without reaching significance. Limitations, reasons for caution The data were drawn from a registry analysis with limited information on patients’ characteristics. Data such as BMI were not available. As the study is based on a register analysis, some errors in data collection cannot be excluded. Wider implications of the findings This is the first large register study to demonstrate a clear association between the three different cycle regimes and pregnancy complications both in singleton and twin pregnancies. HRC-FET showed the highest maternal risks of hypertensive disorders, therefore, it should be avoided and replaced by SC-FET or NC-FET if medically possible. Trial registration number Not applicable.

Study question What is the live birth rate of ovarian tissue transplantation and which factors are associated with the success rate? Summary answer Live birth rate per transplanted woman is 26.5% and the success rate is associated with female age and first versus repeated transplantation. What is known already Live birth rates after tissue transplantations have been reported to be between around 24% and 41% per patient. Success rates seem to be negatively associated with increasing female age at the time of tissue cryopreservation and with pelvic radiation. Success rates are apparently not reduced after overnight transportation of tissue before freezing. Study design, size, duration Registry analysis of 244 transplantations in 196 women, performed by 26 centers of the FertiPROTEKT network from 2007 to 2019 with a follow up till 12/2020. Participants/materials, setting, methods Orthotopic transplantation was performed in 196 women, 191 with previous malignant and 5 with previous non-malignant diseases. Size of transplanting centers varied between 1 and 100 transplantations per center (average: 9.5 ± 19.6 transplantations). Factors possibly associated with success rate such as female age, first and repeated transplantation, experience of the transplanting center and overnight transportation of the tissue before freezing were analysed. Main results and the role of chance Average age of all transplanted 196 women was 31.3 years (SD, standard deviation, 5.2) at the time of cryopreservation (range 17-44y) and 35.9 years (SD 4.8, range 23-47) at the time of first transplantation. Overall pregnancy rate was 30.6% (95% CI, 24.2-37.6%) per first transplantation and 32.7% (95% CI, 26.1-39.7) per patient. Overall live birth rate per first transplantation was 25.0% (95% CI, 19.1-31.7%) and per patient 26.5% (95% CI, 20.5-33.3%). Pregnancy and live birth rates were calculated and graphically expressed as a continuous function of age to allow estimation of success rates per age group. Success rate decreased with increasing age at the time of tissue freezing. Pregnancy rate after first transplantation was 34.5% (95% CI, 26.7-42.9%) in women Pregnancy rate was higher after 1st transplantation (30.6% (95% CI, 24.2-37.6%)) compared to 2nd and more transplantations (11.8% (95% CI, 3.3-27.5%)). Pregnancy rates after 1st transplantation was higher in centers with ≥10 transplantations (35.1%) compared to centers with Success rates was not different in women with and without overnight transportation of tissue before cryopreservation. Limitations, reasons for caution The data were drawn from a registry analysis. Data such as ovarian reserve and premature ovarian insufficiency were not available for all women. Data might be influenced by different follow up policies of the centers. Wider implications of the findings The study reveals the high potential of ovarian tissue freezing and transplantation but only if freezing is performed in younger women. The study suggests to better focus on the first and not on repeated transplantations. It also opens the discussion if transplantation should rather be performed by experienced centres. Trial registration number Non

Study question Does low dose clomiphene citrate (CC) reduce implantation and eventually pregnancy and live birth rates? Summary answer Low dose CC does not reduce implantation rate and pregnancy and live birth rates – based on the model of modified Natural Cycle IVF (NC-IVF). What is known already CC is commonly used for ovulation induction in the treatment of anovulation or idiopathic infertility. It is also added to NC-IVF cycles to mildly stimulate follicular growth and to inhibit LH surge. CC reduces endometrial thickness and affects endometrial function. Therefore it is assumed that CC negatively affects implantation and thereby reduced pregnancy and live birth per embryo. However, previous studies have shown inconsistent results as previous studies mainly calculated pregnancy rates per cycle but not implantation rate per embryo. Study design, size, duration This cohort study included embryos transferred in NC–IVF and CC-NC-IVF cycles. Women with a maximum age of 42 years were treated at a university hospital between 2011 and 2016. Clinical pregnancy rate and live birth rate per embryo transferred and miscarriage rate per amniotic sac were calculated. Participants/materials, setting, methods A daily dose of 25mg of CC starting on day 5-7 of the cycle until the day of ovulation trigger was applied. A modified mixed effect Poisson regression model was used to account for the number of cycles in the same women and the number of embryos in the same cycle to achieve rate ratios (RR). Adjustment for maternal age, parity, type of infertility, duration of subfertility and indication for IVF were made. Main results and the role of chance 499 couples underwent a total of 1043 IVF cycles, 453 NC-IVF and 590 CC-NC-IVF cycles. Baseline characteristics of both groups were not different. The additional administration of CC did neither decrease clinical pregnancy rate (aRR 1.16; 95% CI: 0.90 – 1.50)[MV1] nor live birth rate per transferred embryo (aRR 1.20; 95% CI: 0.89 – 1.62). Miscarriage rate was also not different in CC-NC-IVF compared to NC-IVF (aRR 1.05; 95%: CI 0.63 – 1.76). Limitations, reasons for caution This study does not allow to draw any conclusions regarding the effect of higher CC dosages. As randomization was not performed, some bias regarding the choice of IVF treatment cannot be completely excluded. Wider implications of the findings As this study revealed for the first time that low dose CC does not affect implantation, low does CC can be used for many different treatments. However, further studies are required to evaluate if this also applies to higher CC dosages. Trial registration number not applicable

Background: To estimate sexual orientation associated disparities in sexually transmitted infection (STI)-related outcomes across multiple dimensions of sexual orientation. Methods: Using pooled 20...

Although sexual minority women (SMW) are at risk for cervical cancer and sexually transmitted infections (STIs), they may not seek preventative sexual and reproductive health care at the same rates as their heterosexual peers. We conducted a qualitative descriptive study of 22 adult SMW, a subsample of participants enrolled in the Chicago Health and Life Experiences of Women study. The aim was to describe the sexual and reproductive health literacy of this community sample based on qualitative themes, using an integrated model of health literacy. This model considers not only access to information but also understanding, appraisal, and application of information. We found that family of origin, health care providers, and school-based sexual education were the most important sources of sexual and reproductive health information. Participants described their understanding, appraisal, and application of sexual and reproductive health information as interdependent concepts. Pap test literacy and decision making were strongly independent, with SMW seeking various sources of information, or were driven by health care providers, with SMW following instructions and trusting provider advice. STI-related literacy hinged on whether the participant perceived SMW as at risk for STIs. Our findings reinforce that simply having access to information is insufficient to enact health behaviors that reflect full literacy. Findings have implications for health care providers, who should provide evidence-based recommendations for their SMW clients, and for public health practitioners and educators, who could make sexual health education more inclusive of and specific to the needs of SMW.

It is known that Clomiphene citrate (CC) reduces endometrial thickness, but it is unknown if additional gonadotrophin stimulation increases endometrial thickness and if this has an effect on implantation rate in in vitro fertilization (IVF). The retrospective study included 263 minimal stimulation IVF-cycles stimulated with 25 mg CC per day (CC- IVF), and 161 IVF-cycles stimulated with CC plus 75IU hMG (human Menopausal Gonadotrophin) per day (CC/ hMG-IVF). Endometrial and oestradiol (E2) measurements were analysed between day -4 and 0 (0 = day of oocyte retrieval) and the association of endometrial thickness and treatment on implantation rates were studied after multiple adjustments. It was shown that on day 0, endometrium was significantly thicker in CC/hMG-IVF versus CC-IVF cycles (9.81 ±2.68 versus 9.06 ±2.54 mm, p = 0.005). However, increased endometrial thickness did not have an effect on implantation and live birth rates. In conclusion, gonadotrophins should not be added to low dose CC treated IVF cycles just to increase endometrial thickness as increased endometrial thickness does not increase implantation rate.

Biologically identical menopausal hormone therapy (MHT) including micronized progesterone (MP) has gained much attention. We aimed to assess the impact of MP in combined MHT on venous and arterial thromboembolism (VTE/ATE) (e.g. deep venous thrombosis/pulmonary embolism, myocardial infarction [MI] and ischemic stroke). Articles were eligible if they provided endpoints regarding cardiovascular events and use of exogenous MP. Literature searches were designed and executed for the databases Medline, Embase, CINAHL, the Cochrane Library, ClinicalTrials.gov and interdisciplinary database Web of Science. Twelve studies consisting of randomized controlled trials (RCTs), case-control studies and prospective or retrospective cohort studies were included, and risk of bias was assessed. Only a minority assessed thromboembolic events as a primary endpoint, showing that in contrast to norpregnane derivatives, primary and recurrent VTE risk was not altered by combining estrogens with MP, which was also true for ischemic stroke risk. Similarly, in placebo-controlled RCTs assessing VTE/ATE as adverse events there were no significant intergroup differences. Studies on MI as a primary endpoint are missing. In conclusion, while available data suggest that MP as a component in combined MHT may have a neutral effect on the vascular system, more RCTs investigating the impact of MP alone or in combined MHT on vascular primary endpoints are needed.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited., We present ∼1.5 Mars Years (MY) of ozone vertical profiles, covering LS = 163° in MY34 to LS = 320° in MY35, a period which includes the 2018 global dust storm. Since April 2018, the Ultraviolet and Visible Spectrometer channel of the Nadir and Occultation for Mars Discovery (NOMAD) instrument aboard the ExoMars Trace Gas Orbiter has observed the vertical, latitudinal and seasonal distributions of ozone. Around perihelion, the relative abundance of both ozone and water (from coincident NOMAD measurements) increases with decreasing altitude below ∼40 km. Around aphelion, localized decreases in ozone abundance exist between 25 and 35 km coincident with the location of modeled peak water abundances. High-latitude (>±55°), high altitude (40–55 km) equinoctial ozone enhancements are observed in both hemispheres (LS ∼350°–40°) and discussed in the companion article to this work (Khayat et al., 2021). The descending branch of the main Hadley cell shapes the observed ozone distribution at LS = 40°–60°, with the possible signature of a northern hemisphere thermally indirect cell identifiable from LS = 40°–80°. Morning terminator observations show elevated ozone abundances with respect to evening observations, with average ozone abundances between 20 and 40 km an order of magnitude higher at sunrise compared to sunset, attributed to diurnal photochemical partitioning along the line of sight between ozone and O or fluctuations in water abundance. The ozone retrievals presented here provide the most complete global description of Mars ozone vertical distributions to date as a function of season and latitude. © 2021. The Authors., The NOMAD experiment is led by the Royal Belgian Institute for Space Aeronomy (IASB-BIRA), with Co-PI teams in the United Kingdom (Open University), Spain (IAA-CSIC) and Italy (INAF-IAPS). This work was enabled through UK Space Agency grants ST/V002295/1, ST/P001262/1, ST/V005332/1, ST/S00145X/1 and ST/R001405/1, and this project acknowledges funding by the Belgian Science Policy Office (BELSPO), with the financial and contractual coordination by the ESA Prodex Office (PEA 4000103401, 4000121493), by Spanish Ministry of Science and Innovation (MCIU) and by European funds under grants PGC2018-101836-B-I00 and ESP2017-87143-R (MINECO/FEDER), as well as by the Italian Space Agency through grant 2018-2-HH.0. Support is acknowledged from the STFC under Grant ST/N50421X/1 and The Open University for a PhD studentship. This work was supported by the Belgian Fonds de la Recherche Scientifique – FNRS under grant number 30442502 (ET_HOME). S. A. is “Chargé de Recherches” at the F.R.S.-FNRS. SR thanks BELSPO for the FED-tWIN funding (PRF-2019-077 - RT-MOLEXO). This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 101004052 (RoadMap project). The IAA/CSIC team acknowledges financial support from the State Agency for Research of the Spanish MCIU through the “Center of Excellence Severo Ochoa" award for the Instituto de Astrofísica de Andalucía (SEV-2017-0709). US investigators were supported by the National Aeronautics and Space Administration.

The Emirates Exploration Imager (EXI) on-board the Emirates Mars Mission (EMM) offers both regional and global imaging capabilities for studies of the Martian atmosphere. EXI is a framing camera with a field-of-view (FOV) that will easily capture the martian disk at the EMM science orbit periapsis. EXI provides 6 bandpasses nominally centered on 220, 260, 320, 437, 546, 635 nm using two telescopes (ultraviolet (UV) and visible(VIS)) with separate optics and detectors. Images of the full-disk are acquired with a resolution of 2–4 km per pixel, where the variation is driven by periapsis and apoapsis points of the orbit, respectively. By combining multiple observations within an orbit with planetary rotation, EXI is able to provide diurnal sampling over most of the planet on the scale of 10 days. As a result, the EXI dataset allows for the delineation of diurnal and seasonal timescales in the behavior of atmospheric constituents such as water ice clouds and ozone.This combination of temporal and spatial distinguishes EXI from somewhat similar imaging systems, including the Mars Color Imager (MARCI) onboard the Mars Reconnaissance Orbiter (MRO) (Malin et al. in Icarus 194(2):501–512, 2008) and the various cameras on-board the Hubble Space Telescope (HST; e.g., James et al. in J. Geophys. Res. 101(E8):18,883–18,890, 1996; Wolff et al. in J. Geophys. Res. 104(E4):9027–9042, 1999). The former, which has comparable spatial and spectral coverage, possesses a limited local time view (e.g., mid-afternoon). The latter, which provides full-disk imaging, has limited spatial resolution through most of the Martian year and is only able to provide (at most) a few observations per year given its role as a dedicated, queue-based astrophysical observatory. In addition to these unique attributes of the EXI observations, the similarities with other missions allows for the leveraging of both past and concurrent observations. For example, with MARCI, one can build on the ∼6 Mars years of daily global UV images as well as those taken concurrently with EXI.

Sexually transmitted infections (STI) in lesbian and bisexual women is a relatively unexplored topic, particularly for women from low and middle-income countries. Despite perceptions that women who have sex with women (WSW) are at negligible risk for contracting STI, existing research demonstrates that WSW do become infected with STI. Given the opposition between assumptions of invulnerability and the observed risks, we explored how WSW would respond to symptoms of STI (i.e., wait until symptoms passed, see a medical doctor, and inform sexual partners). We used data collected as part of a collaboration between academic researchers and community-based LGBTQ organizations in Botswana, Namibia, South Africa, and Zimbabwe. Chi-square tests were used to test whether participants’ responses to hypothetical STI symptoms varied in relation to several intrapersonal, interpersonal, and structural factors. Multivariable logistic regression (backward) was used to assess whether these variables were independently associated with women’s responses. Most women would be proactive in response to potential STI symptoms and would see a medical doctor. However, most women would not inform their sexual partner of symptoms of STI. Findings demonstrate several intrapersonal, interpersonal, and structural factors that influence WSW’s health agency, and show a clustering of high-risk factors among women who would not be proactive about their health. Our findings suggest the need for improved health and health care of WSW in Southern Africa.

Purpose: The purpose of this study was to determine the extent to which sexual identity and/or sexual behaviors were associated with pregnancy risk factors (condom use, alcohol or other drug use before sex, and World Health Organization [WHO] Tier 1 [i.e., intrauterine device, implant] or Tier 2 [i.e., injectable, pill, patch, or ring] contraception use) and teen pregnancy among female high school students who reported having a sexual relationship with a male partner. Methods: Data were from the Youth Risk Behavior Survey (YRBS; 2005–2015) (n = 63,313). Logistic regression was used to analyze sexual identity and behavior disparities in pregnancy risk behaviors and teen pregnancy. Interactions between sexual identity and behavior were also tested. All models adjusted for the YRBS complex sampling frame. Results: Girls who reported being unsure of their sexual identity were less likely to use condoms or a WHO Tier 1 or Tier 2 contraceptive method at last sex, and more likely to report alcohol or other drug use at last sex than heterosexual girls. Girls who identified as lesbian were also less likely to use a condom at last sex, and girls who reported both male and female sexual partners were more likely to report alcohol or other drug use and less likely to use condoms at last sex. Girls who identified as bisexual were more likely to report pregnancy during teenage years than girls who identified as heterosexual. Conclusion: Our results support the need to assess both sexual identity and sexual behavior in research on teen pregnancy and pregnancy risk. Furthermore, the finding that girls who were unsure of their sexual identity showed heightened risk highlights the need for additional research that includes this group.

Lesbian, gay, bisexual, transgender, and queer (LGBTQ) populations experience opioid-related disparities compared to heterosexual and cisgender populations. LGBTQ-specific services are needed within opioid use disorder (OUD) treatment settings to minimize treatment barriers; research on the availability and accessibility of such services is limited. The purpose of the current study was to mimic the experience of an LGBTQ-identified individual searching for LGBTQ-specific OUD treatment services, using the SAMHSA National Directory of Drug and Alcohol Abuse Treatment Facilities - 2018 (Treatment Directory).We contacted treatment facilities listed in the Treatment Directory as providing both medications for OUD (MOUD) and "special programs/groups" for LGBTQ clients within states with the top 20 highest national opioid overdose rates. We used descriptive statistics to characterize the outcome of calls; and the overall number of facilities offering LGBTQ-specific services, MOUD, and both LGBTQ-specific services and MOUD in each state by 100,000 state population and in relation to opioid overdose mortality rates (programs-per-death rate).Of the N = 570 treatment facilities contacted, n = 446 (78.25 %) were reached and answered our questions. Of n = 446 reached (all of which advertised both MOUD and LGBTQ-specific services), n = 366 (82.06 %) reported offering MOUD, n = 125 (28.03 %) reported offering special programs or groups for LGBTQ clients, and n = 107 (23.99 %) reported offering both MOUD and LGBTQ-specific services. Apart from Washington, DC, New Mexico, South Carolina, and West Virginia, which did not have any facilities that reported offering both MOUD and LGBTQ-specific services, Illinois had the lowest, and Michigan had the highest programs-per-death rate. Most of the northeastern states on our list (all but New Hampshire) clustered in the top two quarters of programs-per-death rates, while most of southeastern states (all but North Carolina) clustered in the bottom two quarters of programs-per-death rates.The lack of LGBTQ-specific OUD treatment services may lead to missed opportunities for supporting LGBTQ people most in need of treatment; such treatment is especially crucial to prevent overdose mortality and improve the health of LGBTQ populations across the United States, particularly in the southeast.

This study explored factors associated with durable viral suppression (DVS) among two groups of people living with HIV (PLWH) and problem substance use in the context of universal antiretroviral treatment initiation. Participants (N = 99) were recruited between 2014–2017 from public sexual health clinics [SHC] and a hospital detoxification unit [detox]). DVS (NYC HIV surveillance registry) was defined as two consecutive viral load tests ≤ 200 copies/mL, ≤ 90 days apart, with all other viral loads suppressed over 12 or 18 months. Detox participants were significantly older, with more unstable housing/employment, substance use severity, and longer-term HIV vs. SHC participants. Older age, opioid and stimulant use disorder were significantly associated with lower odds of DVS, while fulltime employment and stable housing were significantly associated with higher odds of DVS at 12-month follow-up. Patterns held at 18-month follow-up. Co-located substance use and HIV services, funding for supportive housing, and collaborative patient-provider relationships could improve DVS among populations with the syndemic of problem substance use, poverty, and long-term HIV.

Study question What is the best follicle size, estradiol (E2) serum concentration and endometrial thickness to trigger ovulation in natural cycles? Summary answer Optimal follicles size is 18–22mm but estrogen concentration also need to be considered to maximize oocyte maturity and to minimize premature LH surge. What is known already Timing of the ovulation triggering is essential in infertility treatments based on natural menstrual cycles such as optimized vaginal intercourse, intrauterine inseminations and thawing cycles without hormone replacement therapy. Common parameters to define the day of ovulation triggering are the follicle size and the estrogen concentration. However, data on follicle size and estrogen concentration are either derived from longitudinal evaluations of few ideal participants, are not very detailed or were studied in stimulated cycles. The model of Natural Cycle IVF (NC-IVF) which provides more detailed information has never been used to study this issue. Study design, size, duration Retrospective cross sectional analysis of monofollicular NC-IVF cycles. Follicle size, E2 and LH serum concentrations and endometrial thickness were evaluated on day –5 to 0 (day 0 = day of aspiration). Ovulation was triggered with 5.000IE HCG 36h before aspiration if follicle size was 14–22mm. Patients with irregular cycles, endometriosis >II°, cycles with azoospermia or cryptozoospermia and with inconsistent data were excluded. 606 cycles from 290 women were analysed from 2016 to 2019. Participants/materials, setting, methods Mean age of women undergoing NC-IVF was 35.8±4.0y, median 36y [IQ-range: 34;39]. Each woman performed mean 2.1±1.4, median: 2 [IQ-range: 1–3] NC-IVF cycles at an university based IVF center. All parameters were analysed inter and intraindividually and associations were adjusted for maturity of oocyte, zygote development rate, embryo score, implantation rate and live birth rate. Associations were adjusted for age, cause of infertility and number of previous transfers. Main results and the role of chance Follicle size, E2 concentration and endometrial thickness increased constantly over time. The increase was computed for each cycle without considering any correlation intra patient, revealing an increase of follicle size by 1.04±0.64mm, an increase of E2 concentration by 167.3±76.8pmol/L and endometrial thickness by 0.69±0.59mm per day. Based on a multivariate adjusted model with follicle size, E2 and their interaction, number of retrieved oocytes was associated with E2 concentration (aOR 1.80, 95% CI 1.05–3.11; p = 0.034). Maturity of oocytes was associated not only with E2 concentration (aOR 1.84, 95% CI 1.15–2.94; p = 0.010) but also with follicle size (aOR 1.24, 95% CI 1.01–1.53; p = 0.037) and so was also the interaction of both parameters (aOR 0.96, 95% CI 0.94–0.99; p = 0.017). LH surge was calculated to start in 25% of cases at an E2 level of 545 pmol/l, in 50% of cases at 907pmol/l and in 75% of cases at an E2 level of 1531pmol/l. Live birth rate in cycles with follicles size 14–17 mm was 2.2–3.5% per initiated cycle and in cycles with follicle size 18–22mm 8.5–12.5%. Limitations, reasons for caution Cross sectional studies provides less precise information than longitudinal studies. Follicle size and endometrial thickness were evaluated by several physicians possibly causing some imprecision. Wider implications of the findings: There is a trend towards natural treatment cycles. The study contribute to an optimisation of infertility treatments involving natural cycles. The study gives guidance about the number of days required after a follicle monitoring to reach the optimal time for triggering ovulation. Trial registration number Not applicable