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2. Nanostructured Gold Coating for Prevention of Biofilm Development in Medical Devices

Author

Ariana Zoppi, Diamela María Rocca, M. Cecilia Becerra, Virginia Aiassa, and Jazmin Silvero Compagnucci

Subjects
NANOMATERIALES, medicine.drug_class, Urology, Antibiotics, COATING, 030232 urology & nephrology, Gold coating, Nanotechnology, INGENIERÍAS Y TECNOLOGÍAS, Urinary Catheters, engineering.material, 03 medical and health sciences, 0302 clinical medicine, Coating, purl.org/becyt/ford/2.10 [https], hemic and lymphatic diseases, ANTIBACTERIAL, medicine, Humans, GOLD, Nanotecnología, Infection Control, business.industry, fungi, Biofilm, food and beverages, Equipment Design, Nano-materiales, purl.org/becyt/ford/2 [https], Biofilms, 030220 oncology & carcinogenesis, ANTIBIOFOULING, engineering, Nanoparticles, Gold, BIOFILM, business
Abstract

Bacterial biofilms on medical devices (MDs) can cause deadly infections due to their resistance to antibiotics. Technology to prevent this kind of complication is urgently needed because they impact not only patients' lives but also hospital budgets. In this article, the creation and testing of an easy-to-produce antibiofilm (more precisely antibiofouling) coating are described for the first time. This coating can be applied to catheters, prostheses, and other plastic pieces, even after they have been manufactured. Rapid and ecofriendly synthesis of nanostructured gold coating was done in situ in just 15 minutes. Complete characterization and microbiological analysis of its antibiofouling capacity are presented. The coating prevents biofilm formation of pathogenic clinical isolates and ATCC strains on MDs, possibly due to its complex nanostructured gold surface. If the next generation of MDs is coated with this kind of antibiofouling technology, biofilm-related infections could be dramatically reduced. Fil: Rocca, Diamela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Aiassa, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Zoppi, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Silvero, María Jazmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Becerra, María Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina

Published
2020

4. Author response for 'AT 1 ‐R is involved in the development of long‐lasting, region‐dependent and oxidative stress‐independent astrocyte morphological alterations induced by Ketamine'

Author

Victoria Belén Occhieppo, Natalia Andrea Marchese, Gustavo Baiardi, M. Jazmín Silvero, Claudia Bregonzio, Anahí Rodríguez, M. Cecilia Becerra, Samanta Armonelli, and Osvaldo Martin Basmadjian

Subjects
Long lasting, medicine.medical_specialty, Endocrinology, medicine.anatomical_structure, business.industry, Internal medicine, Medicine, Ketamine, business, medicine.disease_cause, Oxidative stress, medicine.drug, Astrocyte
Published
2020

5. Rapid and effective photodynamic treatment of biofilm infections using low doses of amoxicillin-coated gold nanoparticles

Author

Diamela María Rocca, Virginia Aiassa, and M. Cecilia Becerra

Subjects
Staphylococcus aureus, Biophysics, Metal Nanoparticles, Dermatology, medicine.disease_cause, Microbiology, chemistry.chemical_compound, Extracellular polymeric substance, medicine, Pharmacology (medical), Photosensitizing Agents, biology, Pseudomonas aeruginosa, Acridine orange, Biofilm, Biofilm matrix, Amoxicillin, biology.organism_classification, Antimicrobial, Anti-Bacterial Agents, Oncology, chemistry, Photochemotherapy, Biofilms, Gold, Bacteria
Abstract

Bacterial biofilm are complex microbial communities covered by a matrix of extracellular polymeric substances, which develops when a community of microorganisms irreversibly adheres to a living or inert surface. This structure is considered an important virulence factor because it is difficult to eradicate and often responsible for treatment failures. This adherent community represents one of the greatest problems in public health due to the continued emergence of conventional antibiotic-therapy resistance. Photodynamic Antimicrobial Therapy (PACT) is a therapeutic alternative and promises to be an effective treatment against multiresistant bacteria biofilm, demonstrating a broad spectrum of action. This work demonstrates the reduction in biofilms of relevant clinical isolates (as Pseudomonas aeruginosa and Staphylococcus aureus) treated with PACT using low concentrations of amoxicillin-coated gold nanoparticles (amoxi@AuNP) as a photosensitizer. Moreover, the viability reduction of 60% in S. aureus biofilms and 70% in P. aeruginosa biofilms were obtained after three hours of irradiation with white light and amoxi@AuNP. Scanning electron microscopy analysis revealed that amoxi@AuNP could penetrate and cause damage to the biofilm matrix, and interact with bacteria cells. A strong biofilm production in P. aeruginosa was observed by confocal laser scanning microscopy using acridine orange as a probe, and a markedly decrease in live bacteria was appreciated when PACT was applied. The use of amoxi@AuNP for PACT allows the viability reduction of clinical Gram positive and Gram negative biofilms. This novel strategy needs shorter irradiation times and lower concentrations of nanoparticles than other reports described. This could be attributed to two major innovations: the selectivity for the bacterial wall given by the amoxicillin and the polydispersity of size and shapes with seems to contribute to the photo-antibacterial capacity.

Published
2019

6. Enhanced inhibition of bacterial biofilm formation and reduced leukocyte toxicity by chloramphenicol:β-cyclodextrin:N-acetylcysteine complex

Author

Virginia Aiassa, Marcela Raquel Longhi, Ariana Zoppi, M. Cecilia Becerra, and Inés Albesa

Subjects
Staphylococcus aureus, Β-Cyclodextrin, CIENCIAS MÉDICAS Y DE LA SALUD, Polymers and Plastics, Stereochemistry, Ciencias de la Salud, N-Acetylcysteine, Beta-Cyclodextrins, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, law, Leukocytes, Materials Chemistry, medicine, Humans, Crystal violet, Solubility, Chemiluminescence, chemistry.chemical_classification, Reactive oxygen species, Toxicity, Cyclodextrin, Chloramphenicol, beta-Cyclodextrins, Organic Chemistry, Microbiological Activity, 021001 nanoscience & nanotechnology, Acetylcysteine, 0104 chemical sciences, Enfermedades Infecciosas, chemistry, Biofilms, 0210 nano-technology, medicine.drug, Nuclear chemistry
Abstract

The purpose of this study was to improve the physicochemical and biological properties of chloramphenicol (CP) by multicomponent complexation with β-cyclodextrin (β-CD) and N-acetylcysteine (NAC). The present work describes the ability of solid multicomponent complex (MC) to decrease biomass and cellular activity of Staphylococcus by crystal violet and XTT assay, and leukocyte toxicity, measuring the increase of reactive oxygen species by chemiluminescence, and using 123-dihydrorhodamine. In addition, MC was prepared by the freeze-drying or physical mixture methods, and then characterized by scanning electron microscopy and powder X-ray diffraction. Nuclear magnetic resonance and phase solubility studies provided information at the molecular level on the structure of the MC and its association binding constants, respectively. The results obtained allowed us to conclude that MC formation is an effective pharmaceutical strategy that can reduce CP toxicity against leukocytes, while enhancing its solubility and antibiofilm activity. Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Zoppi, Ariana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Becerra, María Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Albesa, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Longhi, Marcela Raquel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina

Published
2016

7. Biocompatibility and photo-induced antibacterial activity of lignin-stabilized noble metal nanoparticles

Author

Juan C. Scaiano, Diamela María Rocca, Anabel E. Lanterna, Julie P. Vanegas, M. Cecilia Becerra, and Kelsey Fournier

Subjects
Biocompatibility, General Chemical Engineering, NOBLE NANOPARTICLES, Nanoparticle, macromolecular substances, 02 engineering and technology, engineering.material, 010402 general chemistry, complex mixtures, 01 natural sciences, Silver nanoparticle, BIOCOMPATIBILITY, Metal, purl.org/becyt/ford/1 [https], ANTIBACTERIAL, purl.org/becyt/ford/1.4 [https], Chemistry, Otras Ciencias Químicas, technology, industry, and agriculture, Ciencias Químicas, food and beverages, General Chemistry, 021001 nanoscience & nanotechnology, Antimicrobial, 0104 chemical sciences, Colloidal gold, visual_art, visual_art.visual_art_medium, engineering, Noble metal, 0210 nano-technology, Antibacterial activity, CIENCIAS NATURALES Y EXACTAS, Nuclear chemistry, LIGNIN
Abstract

One-pot thermal and photochemical syntheses of lignin-doped silver and gold nanoparticles were developed and their antimicrobial properties were studied against Escherichia coli and Staphylococcus aureus. The nature of the lignin as well as the metal are directly involved in the antimicrobial activity observed in these nanocomposites. Whereas one of the nanocomposites is innocuous under dark conditions and shows photoinduced activity only against Staphylococcus aureus, the rest of the lignin-coated silver nanoparticles studied show antimicrobial activity under dark and light conditions for both bacteria strains. Additionally, only photoinduced activity is observed for lignin-coated gold nanoparticles. Importantly, the particles are non-cytotoxic towards human cells at the bactericidal concentrations. Preliminary assays show these silver nanoparticles as potential antimicrobial agents towards S. aureus biofilm eradication. Fil: Rocca, Diamela María. University Of Ottawa. Faculty Of Science; Canadá. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Vanegas, Julie P.. University Of Ottawa. Faculty Of Science; Canadá Fil: Fournier, Kelsey. University Of Ottawa. Faculty Of Science; Canadá Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Scaiano, Juan Cesar. University Of Ottawa. Faculty Of Science; Canadá Fil: Lanterna, Anabel Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina

Published
2018

8. Effect of antibiotics on cellular stress generated in Shiga toxin-producing Escherichia coli O157:H7 and non-O157 biofilms

Author

Natalia Angel Villegas, M. Cecilia Becerra, José L. Baronetti, M. Gabriela Paraje, Analía Inés Etcheverría, Nora Lía Padola, and Inés Albesa

Subjects
BIOFILMS, Antibiotics, Ciencias de la Salud, Ascorbic Acid, CIPROFLOXACIN, Shiga Toxin 1, Toxicology, medicine.disease_cause, Shiga Toxin 2, Antioxidants, Virulence factor, HEMOLYTIC UREMIC SYNDROME (HUS), Ciprofloxacin, Chlorocebus aethiops, Shiga-Toxigenic Escherichia coli, RIFAXIMIN, Ética Médica, General Medicine, Catalase, Glutathione, Rifamycins, Anti-Bacterial Agents, medicine.drug, CIENCIAS MÉDICAS Y DE LA SALUD, Cell Survival, Virulence Factors, medicine.drug_class, Microbial Sensitivity Tests, Biology, Fosfomycin, Nitric Oxide, Rifaximin, Microbiology, Superoxide dismutase, medicine, Animals, Vero Cells, Escherichia coli, H7 [ESCHERICHIA COLI O157], SHIGA TOXIN (STX), Superoxide Dismutase, Toxin, Biofilm, FOSFOMYCIN, biochemical phenomena, metabolism, and nutrition, Oxidative Stress, Biofilms, biology.protein, Reactive Oxygen Species, Oxidative stress, ANTIBIOTIC
Abstract

Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, with the main virulence factor of this bacterium being its capacity to secrete Shiga toxins (Stxs). Therefore, the use of certain antibiotics for the treatment of this infection, which induces the liberation of Stxs, is controversial. Reactive oxygen and nitrogen species are also involved in the pathogenesis of different diseases. The purpose of this study was to analyze the effects of antibiotics on biofilms of STEC and the relationships between cellular stress and the release of Stx. To this end, biofilms of reference and clinical strains were treated with antibiotics (ciprofloxacin, fosfomycin and rifaximin) and the production of oxidants, the antioxidant defense system and toxin release were evaluated. Ciprofloxacin altered the prooxidant-antioxidant balance, with a decrease of oxidant metabolites and an increase of superoxide dismutase and catalase activity, being associated with high-levels of Stx production. Furthermore, inhibition of oxidative stress by exogenous antioxidants was correlated with a reduction in the liberation of Stx, indicating the participation of this phenomenon in the release of this toxin. In contrast, fosfomycin and rifaximin produced less alteration with a minimal production of Stx. Our data show that treatment of biofilm-STEC with these antibiotics induces oxidative stress-mediated release of Stx. Fil: Angel Villegas, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Baronetti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Albesa, Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquimica y Biotecnologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquimica y Biotecnologia; Argentina Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina

Published
2015

9. Oxidative stress involved in the antibacterial action of different antibiotics

Author

Paulina Laura Páez, Inés Albesa, M. Cecilia Becerra, and Paola C. Battán

Subjects
medicine.drug_class, Antibiotics, Biophysics, Ceftazidime, medicine.disease_cause, Biochemistry, Enterococcus faecalis, Microbiology, Species Specificity, medicine, Molecular Biology, Bacteria, Dose-Response Relationship, Drug, biology, Chemistry, Pseudomonas aeruginosa, Chloramphenicol, Pseudomonas, Cell Biology, biology.organism_classification, Anti-Bacterial Agents, Oxygen, Oxidative Stress, Biofilms, Reactive Oxygen Species, Oxidation-Reduction, Oxidative stress, medicine.drug, Piperacillin
Abstract

Staphylococcus aureus and Escherichia coli sensitive to chloramphenicol incubated with this antibiotic suffered oxidative stress with increase of anion superoxide (O2-). This reactive species of oxygen was detected by chemiluminescence with lucigenin. S. aureus, E. coli, and Enterococcus faecalis sensitive to ciprofloxacin exhibited oxidative stress when they were incubated with this antibiotic while resistant strains did not show stimuli of O2-. Other bacteria investigated was Pseudomonas aeruginosa, strains sensitive to ceftazidime and piperacillin presented oxidative stress in presence of these antibiotics while resistant strains were not stressed. Higher antibiotic concentration was necessary to augment O2- in P. aeruginosa biofilm than in suspension, moreover old biofilms were resistant to oxidative stress caused by antibiotics. A ceftazidime-sensitive mutant of P. aeruginosa, coming from a resistant strain, exhibited higher production of O2- than wild type in presence of this antibiotic. There was relation between antibiotic susceptibility and production of oxidative stress.

Published
2004

10. Rapid and effective photodynamic treatment of biofilm infections using low doses of amoxicillin-coated gold nanoparticles.

Author

Rocca DM, Silvero C MJ, Aiassa V, and Cecilia Becerra M

Subjects
Amoxicillin pharmacology, Anti-Bacterial Agents pharmacology, Biofilms, Gold, Photosensitizing Agents pharmacology, Pseudomonas aeruginosa, Staphylococcus aureus, Metal Nanoparticles, Photochemotherapy methods
Abstract

Bacterial biofilm are complex microbial communities covered by a matrix of extracellular polymeric substances, which develops when a community of microorganisms irreversibly adheres to a living or inert surface. This structure is considered an important virulence factor because it is difficult to eradicate and often responsible for treatment failures. This adherent community represents one of the greatest problems in public health due to the continued emergence of conventional antibiotic-therapy resistance. Photodynamic Antimicrobial Therapy (PACT) is a therapeutic alternative and promises to be an effective treatment against multiresistant bacteria biofilm, demonstrating a broad spectrum of action. This work demonstrates the reduction in biofilms of relevant clinical isolates (as Pseudomonas aeruginosa and Staphylococcus aureus) treated with PACT using low concentrations of amoxicillin-coated gold nanoparticles (amoxi@AuNP) as a photosensitizer. Moreover, the viability reduction of 60% in S. aureus biofilms and 70% in P. aeruginosa biofilms were obtained after three hours of irradiation with white light and amoxi@AuNP. Scanning electron microscopy analysis revealed that amoxi@AuNP could penetrate and cause damage to the biofilm matrix, and interact with bacteria cells. A strong biofilm production in P. aeruginosa was observed by confocal laser scanning microscopy using acridine orange as a probe, and a markedly decrease in live bacteria was appreciated when PACT was applied. The use of amoxi@AuNP for PACT allows the viability reduction of clinical Gram positive and Gram negative biofilms. This novel strategy needs shorter irradiation times and lower concentrations of nanoparticles than other reports described. This could be attributed to two major innovations: the selectivity for the bacterial wall given by the amoxicillin and the polydispersity of size and shapes with seems to contribute to the photo-antibacterial capacity., (Copyright © 2020. Published by Elsevier B.V.)

Published
2020
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