7 results on '"M. Huysamen"'
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2. A recombinant Der p 1-specific allergen-toxin demonstrates superior killing of allergen-reactive IgG+ hybridomas in comparison to its recombinant allergen-drug conjugate
- Author
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A K Daramola, O A Akinrinmade, E A Fajemisin, K Naran, N Mthembu, S Hadebe, F Brombacher, A M Huysamen, O E Fadeyi, R Hunter, and S Barth
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General Medicine - Abstract
Summary Introduction Current treatments for asthma help to alleviate clinical symptoms but do not cure the disease. In this study, we explored a novel therapeutic approach for the treatment of house dust mite allergen Der p 1induced asthma by aiming to eliminate specific population of B-cells involved in memory IgE response to Der p 1. Materials and Methods To achieve this aim, we developed and evaluated two different proDer p 1-based fusion proteins; an allergen-toxin (proDer p 1-ETA) and an allergen-drug conjugate (ADC) (proDer p 1-SNAP-AURIF) against Der p 1 reactive hybridomas as an in vitro model for Der p 1 reactive human B-cells. The strategy involved the use of proDer p 1 allergen as a cell-specific ligand to selectively deliver the bacterial protein toxin Pseudomonas exotoxin A (ETA) or the synthetic small molecule toxin Auristatin F (AURIF) into the cytosol of Der p 1 reactive cells for highly efficient cell killing. Results As such, we demonstrated recombinant proDer p 1 fusion proteins were selectively bound by Der p 1 reactive hybridomas as well as primary IgG1+ B-cells from HDM-sensitized mice. The therapeutic potential of proDer p 1-ETAʹ and proDer p 1-SNAP-AURIF was confirmed by their selective cytotoxic activities on Der p 1 reactive hybridoma cells. The allergen-toxin demonstrated superior cytotoxic activity, with IC50 values in the single digit nanomolar value, compared to the ADC. Discussions Altogether, the proof-of-concept experiments in this study provide a promising approach for the treatment of patients with house dust mite-driven allergic asthma.
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- 2022
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3. Slums of hope: Sanitising silences within township tour reviews
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M. Huysamen, J. Barnett, D.Stanton Fraser, M. Huysamen, J. Barnett, and D.Stanton Fraser
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In a context of rapidly increasing urbanisation and deepening global inequalities, slum tourism has thrived. This paper presents a discursive analysis of tourists’ online reviews of two township tours in Cape Town, South Africa. We investigate how, and to what ends, tourists collectively construct townships as places of hope on TripAdvisor; we question how these reviews feed into broader narratives of urban poverty in the global south. We show how tourists draw on both neoliberal and colonial discourses to construct townships as places of hope–vibrant cultural spaces, rich in non-material assets, inhabited by happy, hard-working residents. We show that by producing slums as productive cultural spaces, tourists are able to resist the stigma associated with slum tourism and position themselves as ethical, enlightened and morally superior tourists. We argue that neoliberal and colonial discourses operate together to produce sanitised representations of townships that both obscure inequalities between poor residents and wealthy tourists from the global north and depoliticise issues like poor infrastructure in townships. In discussing the broader implications of the study, we highlight the importance of producing nuanced representations of townships that acknowledge residents’ assets and excellence, while also foregrounding how they live within the oppressive constraints inherent in enduring systems of inequality.
- Published
- 2020
4. Short report: Recommendations for education, clinical practice, research, and policy on promoting well-being in autistic youth and adults through a positive focus on sexuality and gender diversity.
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Dewinter J, Onaiwu MG, Massolo ML, Caplan R, Van Beneden E, Brörmann N, Crehan ET, Croen LA, Faja S, Gassner DL, Graham Holmes L, Hughes C, Hunter M, Huysamen M, Jelonche P, Lai MC, Noens I, Pukki H, Stokes MA, Strang JF, and van der Miesen AI
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- Adult, Humans, Female, Adolescent, Male, Gender Identity, Sexuality, Policy, Autistic Disorder, Autism Spectrum Disorder
- Abstract
Lay Abstract: In this article, we propose recommendations on what we can do to promote that autistic people can enjoy their sexuality and gender identity, because that contributes to overall well-being.First, we briefly summarize the existing research on sexuality and gender diversity in autistic individuals.Next, we propose recommendations for how to promote sexual and gender diversity-related health and well-being. Based on what is known about sexuality, gender diversity, and relationships in autistic adolescents and adults, we convened an international group of autistic and non-autistic researchers, advocates, parents, and professionals to develop recommendations to promote sexual and gender health in autistic people.The resulting recommendations were checked through an online survey distributed to autistic people across the world. The online participants endorsed the importance of eight final recommendations related to:1. Providing education and information on sexuality, relationships, and gender diversity to autistic individuals and their families;2. Improving expertise in and accessibility to healthcare for sexuality, relationships, and gender-related questions, with specific attention to prevention of and support after sexual victimization; and3. Meaningfully including the autism community in future research that addresses well-being relating to sexuality, relationships, and gender diversity.These community-driven recommendations aim to promote sexual health and well-being in autistic individuals internationally., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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5. When open data closes the door: A critical examination of the past, present and the potential future for open data guidelines in journals.
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Prosser AMB, Hamshaw RJT, Meyer J, Bagnall R, Blackwood L, Huysamen M, Jordan A, Vasileiou K, and Walter Z
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- Humans, Qualitative Research, Dissent and Disputes, Knowledge, Longitudinal Studies, Periodicals as Topic
- Abstract
Opening data promises to improve research rigour and democratize knowledge production. But it also presents practical, theoretical, and ethical considerations for qualitative researchers in particular. Discussion about open data in qualitative social psychology predates the replication crisis. However, the nuances of this ongoing discussion have not been translated into current journal guidelines on open data. In this article, we summarize ongoing debates about open data from qualitative perspectives, and through a content analysis of 261 journals we establish the state of current journal policies for open data in the domain of social psychology. We critically discuss how current common expectations for open data may not be adequate for establishing qualitative rigour, can introduce ethical challenges, and may place those who wish to use qualitative approaches at a disadvantage in peer review and publication processes. We advise that future open data guidelines should aim to reflect the nuance of arguments surrounding data sharing in qualitative research, and move away from a universal "one-size-fits-all" approach to data sharing. This article outlines the past, present, and the potential future of open data guidelines in social-psychological journals. We conclude by offering recommendations for how journals might more inclusively consider the use of open data in qualitative methods, whilst recognizing and allowing space for the diverse perspectives, needs, and contexts of all forms of social-psychological research., (© 2022 The Authors. British Journal of Social Psychology published by John Wiley & Sons Ltd on behalf of British Psychological Society.)
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- 2023
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6. "There's Massive Pressure to Please Her": On the Discursive Production of Men's Desire to Pay for Sex.
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Huysamen M
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- Adult, Aged, Humans, Male, Middle Aged, Remuneration, Sex Workers, South Africa, Masculinity, Men psychology, Motivation, Narration, Sexual Behavior psychology
- Abstract
This article presents a discursive analysis of 43 men's narratives about paying for sex, collected using a combination of online and traditional face-to-face interview methods. It argues that the societal pressures placed on men to "perform" sexually help to produce conditions that make paying for sex desirable. Paying for sex provided men with a "safe" space where they felt exempt from expectations to display sexual experience, skill, and stamina. Moreover, men valued paid sexual encounters with experienced sex workers as spaces where they could acquire sexual experience and skills to better approximate idealised versions of heteronormative male sexuality. The article explores the emotional aspects tied up in men's desires to pay for sex and attends to the question of power within the paid sexual encounter, shedding light on the complexities, nuances and multiplicities within client-sex worker relationships. In conclusion, this paper discusses the value of addressing the broader social structures, sites such as media, online spaces, and medical industries, where heteronormative discourses on male sexual "performance" continue to be reproduced and maintained.
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- 2020
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7. Men's constructions of masculinity and male sexuality through talk of buying sex.
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Huysamen M and Boonzaier F
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- Adult, Aged, Humans, Male, Middle Aged, Qualitative Research, Sexuality, South Africa, Heterosexuality, Masculinity, Men, Sex Work
- Abstract
Commercial sex is an everyday occurrence across a range of contexts in South Africa. In this paper we turn our attention to the often-marginalised role of the buyers of sex by drawing on narrative interviews with male clients of female sex workers recruited through online advertisements in order to explore the ways in which heterosexual men construct, negotiate and perform their masculinity and sexuality through talking about their experiences of paying for sex. We highlight parallels between men's narratives of paying for sex and dominant discourses of gender and heterosexuality. We show how men draw on heteronormative sexual scripts in constructing and making sense of paid sexual encounters and how men are simultaneously able to construct and enact a particular idealised version of masculinity and male sexuality through their talk on paying for sex. Finally, we discuss how online resources could be used more extensively in future research with the male clients of sex workers.
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- 2015
- Full Text
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