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The NASA North Atlantic Aerosols and Marine Ecosystems Study (NAAMES) ship and aircraft field campaign deployed to the western subarctic Atlantic between the years 2015 and 2018. One of the primary goals of NAAMES is to improve the understanding of aerosol–cloud interaction (ACI) over the Atlantic Ocean under different seasonal regimes. ACIs currently represent the largest source of uncertainty in global climate models. During three NAAMES field campaigns (NAAMES-1 in November 2015, NAAMES-2 in May 2016, and NAAMES-3 in September 2017), multiple 10 h science flights were conducted using the NASA C-130 aircraft to measure marine boundary layer aerosol and cloud properties. The standard flight pattern includes vertical spirals where the C-130 transitioned from high altitude to low altitude (and vice versa), collecting in situ measurements of aerosols, trace gases, clouds, and meteorological parameters as a function of altitude. We examine the data collected from 37 spirals during the three NAAMES field campaigns, and we present a comprehensive characterization of the vertical profiles of aerosol properties under different synoptic conditions and aerosol regimes. The vertical distribution of submicron aerosol particles exhibited strong seasonal variation, as well as elevated intra-seasonal variability depending on emission sources and aerosol processes in the atmospheric column. Pristine marine conditions and new particle formation were prevalent in the wintertime (NAAMES-1) due to low biogenic emissions from the surface ocean and reduced continental influence. Higher concentrations of submicron aerosol particles were observed in the spring (NAAMES-2) due to strong phytoplankton activity and the arrival of long-range-transported continental plumes in the free troposphere with subsequent entrainment into the marine boundary layer. Biomass burning from boreal wildfires was the main source of aerosol particles in the region during the late summer (NAAMES-3) in both the marine boundary layer and free troposphere.