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To investigate the diversity and composition of microflora in laboratory-rearedThe larval habitat waters and different developmental stages of laboratory-rearedA total of 16 phyla, 30 classes, 72 orders, 129 families and 224 genera of bacteria were detected in larval habitat waters and different developmental stages ofThere are differences in the composition of symbiotic bacteria at different developmental stages of

Ultralight TiO2 foams have been prepared by an emulsion route using mesoporous TiO2 microbeads (MTMs) as a raw material in water medium followed by mechanical frothing and calcination at elevated temperatures. The MTMs are modified by preferential adsorption of hexadecylamine in ethanol prior to the emulsion practice so that the surface became hydrophobic for facilitating formation of stable air-in-water bubbles. The TiO2 foams are featured with cellular spherical macropores with an interconnected pore structure in a pore-size range from ca. 10 to 400 μm and a porosity ranging from 85.8 to 95.6 % (corresponding to a bulk density of 0.06 to 0.24 g⋅ cm−3). The foams can float on oil/water mixtures and absorb oil selectively in short period of time without noticeable degradation under recycled use, e.g., absorption of soybean oil with up to 15 times of the foam weight has been obtained in less than 30 s after the third use.

1. Birds' newly oviposited blastoderms can survive several weeks in a dormant state during low-temperature storage. Previous studies demonstrated that there is a critical temperature range from 19 to 27°C for chicken embryos. Within this range, the embryo will diapause in a dormant state; once the temperature rises above this range, the blastoderm will break dormancy. 2. Clarifying the mechanism that initiates duck embryo developmental recovery from blastoderm dormancy will be helpful to change temperature control to improve hatching in poultry production. It was hypothesised that there might be some temperature-sensitive genes involved in initiating duck embryo developmental recovery from blastoderm dormancy. 3. To test this hypothesis, the transcriptome of the newly oviposited duck blastoderm and duck embryo (incubated for 48 hours) were sequenced to screen for differentially expressed genes with functions that had been predicted by bioinformatics. 4. The results showed that there were 2416 differentially expressed genes between the two groups, 53 of which were involved in temperature-sensitive pathways. The protein-protein interaction network combined these 53 temperature-sensitive genes and another group of 65 genes, which enriched the development pathway. These results suggested that temperature-sensitive genes may be involved in growth and development related pathways.

To investigate the distribution and density ofDuring the period from June to October in 2018 and 2019, six counties (districts, cities) were sampled in southern, northern and central Jiangsu Province as surveillance sites. The density ofA total of 104 423

To analyze the changes of small molecular metabolites in the larvae of a deltamethrin-sensitive strain ofThe 50% and 75% lethal concentrations (LCThe LCCarbohydrate, carboxylic acid and its derivatives, fatty acyls, amino acids and their derivatives may play important roles in deltamethrin metabolism in the larvae of a deltamethrin-sensitive strain of

An improved AAO process was designed in this study, in which the pre-anaerobic section was prepositioned and water inlet by two-points method was utilized. A regulating section was added between the anaerobic and aerobic sections, and could be switched to anaerobic or aerobic sections as needed to enhance the performance of nitrogen and phosphorus removal. On this basis, the process was applied to the Xihai Sewage Treatment Plant Project (Phase III), aiming to the treatment of domestic sewage with a scale of 8×104 m3/d. Characteristics, mechanisms and design parameters of the process were introduced and the operation results were analyzed in this study. It could be found that the removal efficiencies of COD, NH3-N, TP and SS were 89.36%, 96.89%, 95.99% and 96.78%, respectively. All the effluent indicators meet the first-level A standard in the “Pollutant Discharge Standard for Urban Wastewater Treatment Plant” (18918-2002).

To explore the potential indicators including patients' characteristics, electrocardiogram (ECG), echocardiography, and serological assay in predicting the major adverse cardiovascular events (MACE) within 1 year for patients with low-risk chest pain with a nomogram.The detected indicators of patients with low-risk chest pain were obtained as the alternative predictors for MACE. After the 1-year follow-up, patients with MACE were enrolled in the MACE group while the remained patients were in the non-MACE group. A nomogram was constructed based on the multivariable Cox regression to link the independent predictors and the MACE within 1 year for patients with low-risk chest pain.The incidence of MACE within 1 year was 6.94% according to the follow-up result. Multivariate analysis revealed that risk factors of CAD, P-terminal force in lead V1 (PTFV1), C-reactive protein (CRP), and transmitral inflow early diastolic peak velocity (E wave) /peak early diastolic velocity (Em) (E/Em) were the independent predictors for the MACE. A nomogram incorporating these independent predictors with a good discrimination (0.79 in C-index) and calibration was constructed to predict the incidence of MACE within 1 year. It could be used to help select the patients with a high risk of MACE and develop preventive treatment strategies.Risk factors of CAD, PTFV1, CRP, and E/Em were the independent predictors for the MACE within 1 year in patients with low-risk chest pain. The present nomogram provides a user friendly tool in the prediction of MACE for these patients.

Osteoarthritis (OA) is a common disease in the elderly and seriously affects the quality of life of patients. The purpose of this study was to explore the protective effect of Fibulin-5 on articular chondrocytes and its mechanism of action.Articular cartilage tissues from patients with OA and normal people were selected and tested for differences in Fibulin-5 expression. In addition, human chondrocytes were cultured, and the effects of Fibulin-5 on the extracellular matrix (ECM) of chondrocytes and the level of inflammation were examined by means of cell transfection and cytokine intervention. SKL2001, an agonist of the Wnt/β-catenin signaling pathway, was used to validate the mechanism of action of Fibulin-5 to protect chondrocytes.Fibulin-5 was lowly expressed in the cartilage tissue of patients with OA. Overexpression of Fibulin-5 significantly increased the expressions of ECM collagen II and aggrecan in chondrocytes, while decreasing the expressions of MMP-3 and MMP-13. In addition, Fibulin-5 reduced IL-1β-induced inflammation of chondrocytes, as well as expressions of IL-6, IL-8, and TNF-α. Overexpression of Fibulin-5 also reduced the activity of Wnt/β-catenin signaling pathway, and activation of Wnt/β-catenin signaling pathway attenuated the protective effects of Fibulin-5 on the ECM of chondrocytes.Fibulin-5 can protect the ECM of chondrocytes and reduce the inflammatory response of chondrocytes by inhibiting the Wnt/β-catenin signaling pathway.

The long non-coding RNA DDX11 antisense RNA 1 (DDX11-AS1) was found to be highly expressed in gastric cancer (GC). This study was to explore the role and molecular mechanism in oxaliplatin (OXA) resistance.The levels of DDX11-AS1, microRNA-326 (miR-326) and insulin receptor substrate 1 (IRS1) were measured by quantitative Real-time polymerase chain reaction (qRT-PCR). Cell proliferation, migration, invasion and apoptosis were examined by methylthiazolyldiphenyl-tetrazolium bromide (MTT), transwell and flow cytometry assays, respectively. Levels of all protein were detected using Western blot. The correlation between miR-326 and DDX11-AS1/IRS1 was confirmed by Dual-Luciferase reporter and RNA immunoprecipitation (RIP) assays. The xenograft model was constructed to explore the effect of DDX11-AS1 in vivo.DDX11-AS1 was overexpressed in OXA-resistant GC tissues and cells, and DDX11-AS1 knockdown inhibited cell proliferation, migration, invasion and OXA resistance, and promoted apoptosis in OXA-resistant GC cells. Mechanically, DDX11-AS1 directly targeted miR-326 and miR-326 could bind to IRS1 in OXA-resistant GC cells. Functionally, silencing DDX11-AS1 repressed the progression and OXA resistance in OXA-resistant GC cells by down-modulating IRS1 expression via sponging miR-326 in vitro and in vivo.DDX11-AS1 accelerated the progression and OXA chemoresistance of GC cells in vitro and in vivo by sponging miR-326, thus increasing the expression of IRS1, suggesting DDX11-AS1 might be a promising prognostic biomarker and therapeutic target in GC.

Reactive materials are a new class of energetic materials that extremely and efficiently release energy under the influence of high impact loading. An impact tester is used in the present study to explore the impact ignition characteristics of Al/PTFE reactive materials and the impact ignition pressure of Al/PTFE reaction materials under different conditions. The experimental result shows that the critical ignition pressure is approximately 1.44 GPa. Meanwhile, it also shows that the material compactness has a much less pronounced effect on the impact ignition pressure for this reactive material if the loading time scales are of the order of several milliseconds. Plastic work and viscous heat both play a significant role in impact ignition. Finally, it is shown that impact ignition ensures a higher energy release rate than surface ignition.

Interrupted time series (ITS) is a statistical method for the quasi-experimental design specific to the outcome of time series, in which the effectiveness of an intervening measure is evaluated by examining change in slope and immediate change in level. The key feature of ITS is that the secular trend of中断时间序列（ITS）设计是针对时间序列的结果变量，通过检验干预前后斜率改变量和干预点即刻水平改变量，对干预措施的有效性进行评价的设计方法。ITS的特点是在有效地控制干预前已存在的上升或下降趋势后，更准确地估计干预效应。本文以某综合医院专家挂号费减半对门诊量影响为例，阐述单组ITS的设计原理和统计方法，使用分段线性回归拟合模型，并对结果进行解释。同时，阐述先后存在两种干预的效果评价ITS模型和干预后不同时间点效果评价ITS模型的设计原理、模型拟合方法和结果解释。公共卫生监测数据大多都有时间趋势，为了准确估计干预措施的有效性，需要考虑干预前序列已经存在的上升或下降趋势。ITS通过斜率改变量和即刻水平改变量两个指标评价干预效果，丰富了传统的干预评价模式，在干预效果评价中必有广泛的应用。.

Owing to the distributed features and flexibility, carrier sense multiple access with collision avoidance (CSMA/CA) mechanisms have been widely adopted in wireless networks. Considering energy consumption, we devote to explore an energy efficient carrier sense multiple access with collision avoidance (EE-CSMA/CA) mechanism. The access delay and delay jitter are two of most important quality of service (QoS) metrics. EE-CSMA/CA inevitably experiences the tortures bred from its delay and delay jitter performance, because its frame structure with inactive period brings delay. In this paper, we aim to analyze the access delay and delay jitter of packets for the CSMA/CA with inactive period under the unsaturated conditions. Based on the Markov theory, we model the mechanism of EE-CSMA/CA as a four- dimensional Markov chain, and the fourth variable of Markov chain differentiates the active period and the inactive period of EE-CSMA/CA. Then, deriving in probability generation domain, we get the probability distribution of service time and obtain the access delay. Finally, the queue in a node is decoupled, and two queues with different states of empty and non-empty are configured logically, which helps to enable the complex analysis of delay jitter. Based on the whole probability analysis, the probability distribution of delay jitter is obtained. We show that the active ratio (active time over whole frame time) affects the access delay and delay jitter. When the active ratio is constant, the duration of active period has less effect on the access delay while it deteriorates delay jitter performance.

This study shows how to estimate water content of unsaturated sandy soils from ground-penetrating radar (GPR) data acquired during water drainage experiment. Using the Topp equation, water content can be converted to dielectric permittivity. Afterwards, based on 1D forward modeling method a GPR synthetic trace is generated. Finally, a trial-and-error match of modeled and observed GPR traces was performed to estimate the optimum set of parameters characterizing the materials composing the soil. According to the comparison of the modeling signal and the real signal, we go back to update the water content. Repeating the procedure until the modeling signal fits the real signal very well. We used this procedure to obtain the evolution of water content of unsaturated sandy soils. The method is presented on drainage experiment carried out on unsaturated sandy soil of a large-controlled experimental site. In order to follow the change of the water level, we measured GPR profiles at different times and picked up four GPR profiles, then the same trace (No. 700) were picked up on each GPR profile and estimated the water content from each signal.

The ultra-high pressure piezoresistive sensor works with the Wheatstone half bridge theory. Because of the electric resistance temperature coefficient difference and the four electric resistances value of bridge arm are different in the manufacturing, zero drift and zero temperature drift generally exist in practical application of the ultra-high pressure piezoresistive sensor, which leads to loss of measuring accuracy of sensor. It requires a normalized polynomial fitting algorithm to achieve null balance and compensate zero drift simultaneously. According to model analysis and deduction as well as experiments, statistics obtained through normalized polynomial fitting algorithm is the same as experiments and shows higher accuracy, better compensation effect. The ultra-high pressure piezoresistive sensor compensated by the software method can be widely used in pressure testing and research on interior ballistics, especially the high pressure gun under the high temperature, high pressure, and high impact environment.

Recent studies have revealed that long noncoding RNA DQ786243 (DQ786243) plays an important role in the development of gastric cancer (GC) and colorectal cancer. However, the expression and function of DQ786243 in GC patients remain largely unknown. The purpose of our study was to investigate the clinical significance of DQ786243 expression in GC.qRT-PCR was used to examine DQ786243 expression in 172 paired GC samples and matched adjacent normal tissues. Besides, the relationship between DQ786243 expression and clinicopathologic characteristics was analyzed. Overall survival (OS) and progression-free survival (PFS) curves of patients in subgroups were constructed by the Kaplan-Meier method. Multivariate regression analyses were performed to analyze independent factors affecting prognosis.We found a significant up-regulation of DQ786243 in GC tissues compared to normal tissues (p0.01). High DQ786243 expression was closely associated with invasion depth (p = 0.006), TNM stage (p = 0.009) and lymphatic metastasis (p = 0.017). Moreover, Kaplan-Meier analysis showed that patients with DQ786243 high expression tumors had worse OS (p = 0.0012) and PFS (p = 0.0002) compared to patients with DQ786243 low expression tumors. Finally, multivariate analysis showed that DQ786243 was a significant and independent prognostic predictor for both OS (p = 0.002) and PFS (p = 0.001) of GC patients.Our data suggest, for the first time, that the evaluation of the DQ786243 expression in GC tissues is a useful tool for predicting prognosis of GC.

The objective of this study was to investigate the effects of immunological challenge on the skeletal muscle fiber type conversion of piglets. Sixteen Large White weaned barrows (28 ± 3 d, 8.22 ± 0.89 kg BW) were allotted by weight and litter to 2 groups: the control group and the lipopolysaccharide (LPS) group. Saline (control) or LPS was injected intravenously via a jugular catheter on d 1, 3, 5, 7, 9, 11, 13, and 15 at an initial dosage of 80 μg/kg BW, which was increased by 30% at each subsequent injection. Blood samples were collected via the jugular catheter 3 h after the LPS challenge on d -1, 1, 5, 9, and 13. Muscle tissue samples were collected from the LM after exsanguination on d 15. The LPS challenge increased the plasma IL-6, tumor necrosis factor-α (TNF-α), cortisol, IL-1β, and haptoglobin concentrations on d 1 and 5 ( < 0.01) and increased the plasma IL-6 ( < 0.05), TNF-α ( < 0.05), and haptoglobin ( < 0.01) levels on d 9. Compared with that of the control group, the ADG of the LPS group decreased by 40.00% ( < 0.01), 29.52% ( < 0.05), and 19.30% ( < 0.05), and the ADFI decreased by 25.09% ( < 0.01), 23.15% ( < 0.05), and 19.47% ( < 0.05) during d 1 to 4, d 5 to 8, and d 9 to 15, respectively. In the LM of LPS-challenged piglets, myosin heavy chain 1 (MyHC1) mRNA and protein expression tended to be reduced ( = 0.08, 0.09), whereas mRNA, mRNA, and MyHC2 protein expression increased ( < 0.05). The LPS challenge reduced succinic dehydrogenase (SDH) activity ( < 0.05) and increased lactate dehydrogenase (LDH) activity ( < 0.05) in the LM of piglets. Compared with those in the control group, transcriptional peroxisome proliferator-activated receptor coactivator-α () mRNA ( < 0.05), calcineurin (CaN) mRNA, and protein expression were reduced ( < 0.05), and PGC-α protein expression tended to be reduced ( = 0.08) in the LM of LPS-challenged piglets. These results show that immunological challenge induced by LPS resulted in a shift from type I to type II fibers in the LM of piglets, which may be mediated by the downregulation of the CaN/PGC-α signaling pathway.

This study was undertaken to investigate the effects of ambient temperature, crude protein levels and their interaction on performance and serum biochemical parameters of broiler chickens. A total of 216 Arbor Acre broiler chickens (108 males and 108 females) were used in a 2 × 3 factorial arrangement and randomly reared at two temperatures (normal temperature: 23 °C; daily cyclic high temperature: 28-32 °C) and fed on three diets with different crude protein levels (153.3, 183.3 or 213.3 g/kg, with constant essential amino acids) from 28 to 42 days of age. Daily cyclic high ambient temperature decreased final body weight, average daily weight gain, average daily feed intake and serum total protein contents (p

This is the first time a series of chemically homogeneous superlattice Cu7.2Sx (x = 4.07, 4.52, 6.01, 6.20, 6.45) nanowires have been successfully synthesized by heavy doping of S2 species in Cu7.2S4 lattice through a simple wet-chemical route. This superlattice structure is a polytypoid structure tuned by adjusting the atom ratio of S2 to S in lattice configuration. The perfect superlattice Cu7.2S6.20 structure interestingly consists of two alternating lattice fringes corresponding to the atom layers of Cu–S and Cu–S2 in an even spacing of 5.70 A. The article describes the formation, morphology, composition and structure of the Cu7.2Sx superlattice nanowires. Photoluminescence (PL) spectra and transient photovoltage (TPV) measurements reveal that the generation and separation efficiency of the photogenerated charges of Cu7.2Sx nanowires could be greatly improved by adjusting the S2/S ratio in the lattice configuration, and thus enhance the luminescence quantum efficiency. This study reveals that the S2 species in Cu7.2Sx nanowires play a very important role in determining the dynamic properties of photogenerated charge carriers.

The fatigue behaviour of A356 cast aluminium alloy under 104 cycles was investigated by a servo-hydraulic machine at the temperature of 150°C. The specimens for the fully reversible tension compression tests were cut from cylinder head castings of automobile engines. The testing data were treated by S–N curve and the staircase method and the mean values of fatigue property coincided with each other by these two methods, though the data scattered with different casting defects. The fracture surfaces of fatigue specimens were examined by OM, SEM and EDS. It was found that in most of the specimens two or more fatigue crack initiations appeared at a fracture surface. Compared with casting defects such as oxide film, inclusion and blowhole, shrinkage porosity is more detrimental to the fatigue behaviour of aluminium alloy castings.

Objective Acquired aplastic anaemia (AA) is a T-cell-mediated, organ-specific autoimmune disease characterized by haematopoietic stem cell destruction in the bone marrow. The exact molecular mechanism of T-cell trafficking into the bone marrow is unclear in AA. Very late activation antigen-4 (VLA-4) and CX3C chemokine receptor 1 (CX3CR1) play active roles in many autoimmune diseases. Therefore, we investigated whether VLA-4 and CX3CR1 also contribute to T-cell migration into the bone marrow in acquired AA. Design, setting and subjects Expression levels of CX3CR1 and VLA-4 and their ligands [fractalkine (CX3CL1) and vascular cell adhesion molecule-1 (VCAM-1)] were examined in 63 patients with AA and 21 healthy control subjects. T-cell chemotaxis and adhesion were analysed in 17 patients with severe AA. We also prospectively evaluated the expression pattern of CX3CR1 during treatment with antithymocyte globulin plus cyclosporine in 11 patients with severe AA. Results The proportion of peripheral and bone marrow CD4+ and CD8+ T cells expressing CX3CR1 and the level of CX3CL1 was increased in patients with AA. However, there was no significant difference in VLA-4 expression or VCAM-1 levels. Functional studies demonstrated that chemotaxis towards autologous bone marrow plasma or soluble CX3CL1 was significantly higher in T cells from AA patients and could be blocked by CX3CR1 inhibitors. CX3CR1-mediated T-cell adhesion was also upregulated in these patients. The expression of CX3CR1 was associated with the efficacy of immunosuppressive therapy. Conclusion The present findings demonstrate that CX3CR1 plays a pivotal role in recruitment of T cells into the bone marrow in acquired AA and is a potential therapeutic target for treatment of this disorder.

Electrical, thermal and mechanical properties of conductive polymer composites were investigated in the present work. The composites were polyamide-6 (PA6)/stainless steel fibre (SSF) (PA6 matrix filled by SSFs) and PA6/SSF/CNT (PA6 matrix filled by SSFs and carbon nanotubes) systems. The distribution of fillers in the composites was observed by scanning electron microscopy. The relation between the resistivity of the composites and the mass fraction of fillers was determined, and the conductive percolation threshold value was approximately evaluated according to the sudden dropping of the resistivity. Thermal and mechanical properties of the composites were investigated by means of dynamic mechanical analysis, thermogravimetric analysis and differential scanning calorimeter analysis.

Fine tuning of concrete recycling processes requires a comprehensive understanding of the overlapping interactions and effects of the various influencing parameters. The most important parameters associated with recycled concrete aggregate (RCA) production that may affect quality and yield include such properties of the parent concrete as the composition, strength and aggregate grading, type of crushers used, number of crushing stages, the size of the RCA particles, and the size reduction sequence. The effects of such parameters overlap and interact with one another and render it impossible for firm conclusions to be drawn on the effects of one parameter in isolation as evidenced by results reported in available literature. The present paper reports on the findings of an experimental study on the concomitant effects of the strength of the parent concrete, size of the natural aggregates used in the parent concrete, and the number of crushing stages on the properties of coarse recycled concrete aggr...

Poster presentada en el 50th ERA-EDTA Congress (European Renal Association - European Dialysis Transplant Association), celebrado del 18 al 21 de mayo de 2013 en Estambul (Turquia)

Myocardial constitutive No production depends on the activity of both endothelial and neuronal NOS (eNOS and nNOS, respectively). Stimulation of myocardial β(3)-adrenergic receptor (β(3)-AR) produces a negative inotropic effect that is dependent on eNOS. We evaluated whether nNOS also plays a role in β(3)-AR signaling and found that the β(3)-AR-mediated reduction in cell shortening and [Ca(2+)](i) transient amplitude was abolished both in eNOS(-/-) and nNOS(-/-) left ventricular (LV) myocytes and in wild type LV myocytes after nNOS inhibition with S-methyl-l-thiocitrulline. LV superoxide (O(2)()) production was increased in nNOS(-/-) mice and reduced by l-N(ω)-nitroarginine methyl ester (l-NAME), indicating uncoupling of eNOS activity. eNOS S-glutathionylation and Ser-1177 phosphorylation were significantly increased in nNOS(-/-) myocytes, whereas myocardial tetrahydrobiopterin, eNOS Thr-495 phosphorylation, and arginase activity did not differ between genotypes. Although inhibitors of xanthine oxidoreductase (XOR) or NOX2 NADPH oxidase caused a similar reduction in myocardial O(2)(), only XOR inhibition reduced eNOS S-glutathionylation and Ser-1177 phosphorylation and restored both eNOS coupled activity and the negative inotropic and [Ca(2+)](i) transient response to β(3)-AR stimulation in nNOS(-/-) mice. In summary, our data show that increased O(2)() production by XOR selectively uncouples eNOS activity and abolishes the negative inotropic effect of β(3)-AR stimulation in nNOS(-/-) myocytes. These findings provide unequivocal evidence of a functional interaction between the myocardial constitutive NOS isoforms and indicate that aspects of the myocardial phenotype of nNOS(-/-) mice result from disruption of eNOS signaling.

To report experiences and results of complex aortic aneurysms involving the visceral arteries treating by total endovascular repair.Seventy cases of thoracoabdominal aortic lesions treating by total endovascular repair in Department of Vascular Surgery, People's Liberation Army General Hospital from January 2011 to December 2014 were retrospectively analyzed. There were 47 cases underwent chimney technique, 6 underwent sandwich technique, 15 underwent fenestration technique and 2 underwent branched stent grafts technique.The average follow-up time was 21 months, range from 3 to 47 months. Completion angiography showed that typeⅠ endoleak of chimney, sandwich, fenestration and branched stent grafts group were 9/47, 5/6, 2/15 and 0, respectively. While at 30-day typeⅠ endoleak reduced to 7/47, 2/6, 0 and 0. During follow-up, there were 3 target vessel stents occlusion in chimney group, the patency rate of target vessel stent was 95.1% (58/61). In sandwich, fenestration and branched stent grafts group, all the target vessel stents kept patent.In this study, chimney, sandwich, fenestration and branched stent grafts techniques show good short-term and midterm results, the long-term effects still need further studies.