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Objetivos Este proyecto experimental ha sido disenado para evaluar: a) la capacidad de la oxitetraciclina para inducir esteatosis en el higado de rata cuando es administrada durante un periodo prolongado; b) evaluar si las ratas hembras son mas susceptibles a este farmaco, y c) estudiar las posibles alteraciones ultraestructurales y su relacion con los mecanismos de la esteatosis. MeTodos Sesenta y dos ratas Wistar (31 machos y 31 hembras) fueron distribuidas en 6 grupos, dos grupos control y cuatro grupos experimentales. El estudio duro 3 meses. Se obtuvo sangre y tejido hepatico bajo anestesia para el estudio morfologico (microscopia optica [MO] y microscopia electronica [ME]). Resultados La esteatosis fue de tipo microvesicular con una distribucion periportal. Aparecio en los grupos tratados, con un grado significativamente mayor en las hembras (p = 0,004). No se observo ninguna relacion con la dosis. El estudio ultraestructural revelo una dilatacion de los microsomas en todos los grupos experimentales, sin diferencias en cuanto al sexo. No se constato una proliferacion de los peroxisomas ni la aparicion alteraciones mitocondriales. Conclusiones La oxitetraciclina produce predominantemente esteatosis hepatica microvesicular, apareciendo fundamentalmente en hembras. Como posible mecanismo de esta esteatosis inducida por oxitetraciclina postulamos un descenso en las funciones mitocondrial, microsomal y peroxisomal como resultado de una inhibicion en la sintesis proteica de estos compartimientos celulares.

Objective Watching if long acting testosterone added to sildenafil can maintain improved sexual life, reflected by a longest erectile capability and sexual desire, in erectile dysfunction (ED) associated with metabolic syndrome (MetS). Design and method In 49 men with MetS (NCEP-ATP III criteria) ED was assessed (V1) using IIEF questionnaire and its domains: erectile function (EF) and sexual desire (SD). ED was treated by sildenafil citrate (S) in 2-4 tb. of 50 mg/week for 2 months. Group A = 24 participants receiving also a long acting testosterone (T) injection. Group B = 25 cases treated only with sildenafil. At 10 days (V2) and 30 days (V3) and immediate (V2) and at 30 days (V3) after S end usage EF and SD respectively were assessed. Results IIEF (V1): Gr. A vs. Gr. B = 44.16 vs. 43.67 points (p). EF domain scores: Gr. A: V1=16.2 p; V2=26.34 p (P=0.002); V3=20.89 p (P=0.07). Gr. B: V1=14.1 p; V2=23.3 (P=0.001); V3=15.9 (P=0.5). V2: Gr. A vs. Gr. B=26.34 vs. 23.3 (P=0.45). V3: Gr. A vs. Gr. B=20.89 vs. 15.9 (P=0.07). SD domain scores: Gr. A: V1=4.7 p; V2=8.9 p (P=0.001); V3=8.5 p (P=0.08). Gr. B: V1=5.7 p; V2=9.1 p (P=0.002); V3=4.9 p (P=0.15). V2: Gr. A vs. Gr. B=8.9 vs. 9.1 (P=0.6); V3: Gr. A vs. Gr. B=8.5 vs. 4.9 (P=0.001). Conclusions Long acting testosterone really improve sexual life by prolonging a satisfying prevalence of non phosphodiesteraze-5 inhibitor induced erections and maintaining sexual desire after sildenafil usage.

We have studied kidney samples of 16 garden dormouses (Eliomys quercinus L.) divided into two groups, 8 hibernating and 8 non-hibernating. Hibernation produces structural modifications in the glomerular ultrafilter: (1) the endothelial pores decrease in number and size; (2) the podocytic food processes increase in number and the slit pores decrease in size; (3) in the basement membrane there are no structural morphological modifications. In short, we could say that the permeability of the glomerular ultrafilter decreases during hibernation. This fact helps to understand the decrease in the rate of urine formation that takes place in the presence of a low body temperature and a metabolic depression.

Summary The effects of oral administration of valproic acid (VPA) have been studied on the liver of female adult Wistar rats, at doses of 60 and 120 mg/kg/day during 4 and 18 weeks (periods I and II). Other groups received the same doses of VPA with phenobarbital (PB). The animals developed periportal steatosis, more intense at the end of period II and in groups non-induced by PB, and also some degree of mitochondrial swelling. An uncoupling of the oxidative phosphorylation was also evident with both doses in the non-induced groups, and only with the high dose of VPA in the induced group. An in vitro study on isolated liver mitochondria showed uncoupling only with very high concentration of VPA (10 mM). We comment on these findings and propose a chronic model of experimental hepatotoxicity of oral VPA in the rat.

We have carried out an ultrastructural study of the proximal convoluted kidney tubule in hibernating and nonhibernating garden dormice. Deep hibernation produced the following changes: the nuclei had an irregular shape; saccular cavities appeared near the nucleus and there was an increase in the components of the lysosomal system; hibernation seems to have no influence on the number of mitochondria; hypertrophic mitochondria occur in controls, whereas none were ever seen in hibernating dormice.