1. Listeriolysin S: A bacteriocin from Listeria monocytogenes that induces membrane permeabilization in a contact-dependent manner
- Author
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Meza-Torres, Jazmín, Lelek, Mickaël, Quereda, Juan, Sachse, Martin, Manina, Giulia, Ershov, Dmitry, Tinevez, Jean-Yves, Radoshevich, Lilliana, Maudet, Claire, Chaze, Thibault, Giai Gianetto, Quentin, Matondo, Mariette, Lecuit, Marc, Martin-Verstraete, Isabelle, Zimmer, Christophe, Bierne, Hélène, Dussurget, Olivier, Cossart, Pascale, Pizarro-Cerdá, Javier, Yersinia, Institut Pasteur [Paris] (IP), Interactions Bactéries-Cellules, Université Paris Cité (UPCité), Université Sorbonne Paris Cité (USPC), Imagerie et Modélisation - Imaging and Modeling, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidad Cardenal Herrera-CEU (CEU-UCH), Plateforme BioImagerie Ultrastructurale – Ultrastructural BioImaging Platform (UTechS UBI), Individualité microbienne et infection - Microbial Individuality and Infection, Hub d'analyse d'images - Image Analysis Hub (Platform) (IAH), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, University of Iowa [Iowa City], Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes (PBA (U-Pasteur_6)), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This work received financial support from Institut Pasteur, Institut Carnot Pasteur Microbes et Santé (PasteurInnov Grant LLS-Bact), Région Ile-de-France (DIM-MALINF), and Fondation pour la Recherche Médicale (Grant FDT201904008152) (to J.M.-T.). J.J.Q. is supported by a 'Ramón y Cajal' contract from the Spanish Ministry of Science, Innovation and Universities (RYC-2018-024985-I). The Yersinia Research Unit is a member of the LabEX Integrative Biology of Emerging Infectious Diseases (ANR LBX-62 IBEID), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Bierne, Hélène, and Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID
- Subjects
MESH: Cytoplasm ,[SDV]Life Sciences [q-bio] ,microfluidic microscopy ,MESH: Listeria monocytogenes ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,contact-dependent inhibition (CDI) ,[SDV] Life Sciences [q-bio] ,MESH: Bacteriocins ,bacteriocin ,MESH: Hemolysin Proteins ,Listeria monocytogenes (Lm) ,MESH: Adenosine Triphosphate ,Listeriolysin S (LLS) ,[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Cell Membrane - Abstract
International audience; Significance Listeria monocytogenes ( Lm ) is a bacterial pathogen that causes listeriosis, a foodborne disease characterized by gastroenteritis, meningitis, bacteremia, and abortions in pregnant women. The most severe human listeriosis outbreaks are associated with a subset of Lm hypervirulent clones that encode the bacteriocin Listeriolysin S (LLS), which modifies the gut microbiota and allows efficient Lm gut colonization and invasion of deeper organs. Our present work identifies the killing mechanism displayed by LLS to outcompete gut commensal bacteria, demonstrating that it induces membrane permeabilization and membrane depolarization of target bacteria. Moreover, we show that LLS is a thiazole/oxazole–modified microcin that displays a contact-dependent inhibition mechanism.
- Published
- 2021
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