Descriptor: "MESH: Contrast Media" - Searchworks@Jio Institute Digital Library Search Results

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109 results on '"MESH: Contrast Media"'

1. Are Gadolinium-Enhanced MR Sequences Needed in Simultaneous 18F-FDG-PET/MRI for Tumor Delineation in Head and Neck Cancer?

Author: E. Chambenois, Didier Dormont, Aurélie Kas, S. Bergeret, Nadya Pyatigorskaya, R. De Laroche, G. Herve, C. Bertolus, M. Amor-Sahli, Alain Giron, G. Bera, Colliot, Olivier, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Morvan - CHRU de Brest (CHU - BREST ), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire d'Imagerie Biomédicale (LIB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Algorithms, models and methods for images and signals of the human brain (ARAMIS), Sorbonne Université (SU)-Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Laboratoire d'Imagerie Biomédicale [Paris] (LIB)
Subjects: [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Gadolinium, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, chemistry.chemical_element, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, 18f fdg pet, 03 medical and health sciences, [INFO.INFO-CV] Computer Science [cs]/Computer Vision and Pattern Recognition [cs.CV], 0302 clinical medicine, Text mining, MESH: Fluorodeoxyglucose F18, MESH: Contrast Media, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, medicine, Radiology, Nuclear Medicine and imaging, Head and neck, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, MESH: Aged, MESH: Humans, MESH: Middle Aged, business.industry, Head and neck cancer, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [INFO.INFO-CV]Computer Science [cs]/Computer Vision and Pattern Recognition [cs.CV], MESH: Adult, MESH: Retrospective Studies, medicine.disease, Mr imaging, MESH: Male, MESH: Positron-Emission Tomography, MESH: Sensitivity and Specificity, 3. Good health, MESH: Head and Neck Neoplasms, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, [INFO.INFO-TI] Computer Science [cs]/Image Processing [eess.IV], chemistry, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], Neurology (clinical), MESH: Gadolinium, business, Nuclear medicine, MESH: Female, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, MESH: Radiopharmaceuticals, 030217 neurology & neurosurgery
Abstract: International audience; PET/MRI with 18F-FDG has demonstrated the advantages of simultaneous PET and MR imaging in head and neck cancer imaging, MRI allowing excellent soft-tissue contrast, while PET provides metabolic information. The aim of this study was to evaluate the added value of gadolinium contrast-enhanced sequences in the tumor delineation of head and neck cancers on 18F-FDG-PET/MR imaging.
Published: 2020
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2. Pseudo-continuous arterial spin labelling shows high diagnostic performance in the detection of postoperative residual lesion in hyper-vascularised adult brain tumours

Author: Marc Sanson, Bruno Law-Ye, Nadya Pyatigorskaya, Delphine Leclercq, Didier Dormont, Clara Cohen, Laurent Capelle, Damien Galanaud, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Algorithms, models and methods for images and signals of the human brain (ARAMIS), Sorbonne Université (SU)-Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, Neoplasm, Residual, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Contrast Media, Perfusion scanning, Neurosurgical Procedures, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, MESH: Postoperative Period, Medicine, Postoperative Period, Prospective Studies, Neuroradiology, MESH: Middle Aged, medicine.diagnostic_test, Brain Neoplasms, Ultrasound, General Medicine, MESH: Cerebrovascular Circulation, Middle Aged, Perfusion imaging, Postoperative procedures, Cerebral blood flow, Cerebrovascular Circulation, 030220 oncology & carcinogenesis, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], MESH: Brain Neoplasms, Disease Progression, Female, MESH: Disease Progression, Radiology, medicine.symptom, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Adult, medicine.medical_specialty, education, Lesion, Residual tumour, 03 medical and health sciences, McNemar's test, Magnetic resonance imaging, MESH: Contrast Media, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, Radiology, Nuclear Medicine and imaging, MESH: Neoplasm, Residual, MESH: Humans, business.industry, Brain tumours, [INFO.INFO-CV]Computer Science [cs]/Computer Vision and Pattern Recognition [cs.CV], MESH: Adult, MESH: Neurosurgical Procedures, Perioperative, MESH: Male, MESH: Prospective Studies, business, MESH: Female
Abstract: Our aim was to evaluate the contribution of pseudo-continuous arterial spin labelling (pCASL) in the detection of a postoperative residual lesion in adult brain tumours. Seventy-five patients were prospectively included. Following the results of preoperative DSC-PWI assessment, intra-axial lesions, including high-grade gliomas (n = 43) and certain metastases (n = 14), were classified as hyper-vascular (HV+ group, n = 57); other lesions, including low-grade gliomas and certain metastases, were classified as non-hyper-vascular (HV− group, n = 18). To confirm the absence/presence of a residual lesion or disease progression, postoperative MRI including pCASL sequence and follow-up-MRI were performed within 72 h and 1–6 months after the resection, respectively. Two raters evaluated the images. Mean and maximal ASL cerebral blood flow (CBF) values were measured in the perioperative region and normalised to the contralateral tissue. The pCASL-CBF maps and post-contrast T1WI were visually assessed for residual lesion. Quantitative data were analysed with unpaired Student t and Mann-Whitney U tests and the visual diagnostic performance with the McNemar test. In the HV+ group, the mean normalised CBF was 1.97 ± 0.59 and 0.97 ± 0.29 (p < 0.0001, AUC = 0.964, cut-off = 1.27) for patients with or without residual tumours, respectively. The mean normalised CBF was not discriminative for assessing residual tumours in the HV− group (p = 0.454). Visual CBF evaluation allowed 92.98% patients belonging to the HV+ group to be correctly classified (sensitivity 93.02%, specificity 92.86%, p < 0.001). Visual evaluation was correlated with contrast enhancement evaluation and with the mean normalised CBF values (r = 0.505, p < 0.0001 and 0.838, p < 0.0001, respectively). Qualitative and quantitative ASL evaluation shows high diagnostic performance in postoperative assessment of hyper-perfused tumours. In this case, postoperative pCASL may be useful, especially if contrast injection cannot be performed or when contrast enhancement is doubtful. • Evaluation of postoperative residual lesion in the case of brain tumours is an imaging challenge. • This prospective monocentric study showed that increased normalised cerebral blood flow assessed by pseudo-continuous arterial spin labelling (pCASL) correlates well with the presence of a residual tumour in the case of hyper-vascular tumour diagnosed on preoperative MRI. • Qualitative and quantitative pCASL is an informative sequence for hyper-vascular residual tumour, especially if acquired more than 48 h after brain tumour surgery, when contrast enhancement can give ambiguous results due to blood-brain barrier disruption.
Published: 2020
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3. Breast MRI: Is Faster Better?

Author: Asma Bekhouche, Cendos Abdel Wahab, Edith Kermarrec, Audrey Milon, Patrice Taourel, Isabelle Thomassin-Naggara, Institut des Sciences du Calcul et des Données (ISCD), Sorbonne Université (SU), CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Radiologie [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Hôpital Lapeyronie [Montpellier] (CHU)
Subjects: medicine.medical_specialty, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Contrast Media, Breast Neoplasms, [SDV.CAN]Life Sciences [q-bio]/Cancer, Sensitivity and Specificity, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 03 medical and health sciences, MESH: Clinical Protocols, 0302 clinical medicine, Breast cancer, Clinical Protocols, Abbreviated, MESH: Contrast Media, medicine, Breast MRI, Humans, Mass Screening, MESH: Early Detection of Cancer, Radiology, Nuclear Medicine and imaging, Screening tool, Breast density, Breast, MESH: Mass Screening, [STAT.CO]Statistics [stat]/Computation [stat.CO], Early Detection of Cancer, Cancer, Protocol (science), MESH: Humans, medicine.diagnostic_test, business.industry, General Medicine, medicine.disease, Predictive value, Magnetic Resonance Imaging, MESH: Sensitivity and Specificity, 3. Good health, 030220 oncology & carcinogenesis, Female, Radiology, Intermediate risk, business, MESH: Female, MESH: Breast Neoplasms, MRI
Abstract: International audience; OBJECTIVE. Fast breast MRI protocols have the same sensitivity as conventional protocols, but their specificity is variable and can be inadequate. An ultrafast sequence provides early enhancement of lesion characteristics that optimize the characterization of the fast protocol, increasing positive predictive values without increasing time. CONCLUSION. These new abbreviated protocols could constitute a viable screening tool both for women at high risk of breast cancer and for those at intermediate risk with high breast density.
Published: 2020
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4. Impact of gadolinium-based contrast agents on the growth of fish cells lines

Author: Justine Flayac, Marc Parant, E. Perrat, Carole Cossu-Leguille, Bénédicte Sohm, Christophe Rosin, F. Chuburu, C. Cadiou, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), ANR-10-LABX-0021/10-LABX-0021,RESSOURCES21,Strategic metal resources of the 21st century(2010), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'hydrologie de Nancy (LHN), and ANR-10-LABX-0021,RESSOURCES21,Strategic metal resources of the 21st century(2010)
Subjects: Gadolinium DTPA, MESH: Heterocyclic Compounds, Cell Survival, Health, Toxicology and Mutagenesis, Gadolinium, ZF4 cell line, Contrast Media, chemistry.chemical_element, 010501 environmental sciences, 01 natural sciences, Cell Line, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, chemistry.chemical_compound, Cell growth, Meglumine, 0302 clinical medicine, Heterocyclic Compounds, MESH: Contrast Media, Organometallic Compounds, Animals, DOTA, Chelation, Zebrafish, Chelating Agents, 0105 earth and related environmental sciences, Doratem®, Diminution, Chemistry, Cell Cycle, Public Health, Environmental and Occupational Health, MESH: Organometallic Compounds, General Medicine, Magnetic Resonance Imaging, Pollution, MESH: Water Pollutants, Chemical, Contrast agent, Cell culture, Gadoteric acid, Oncorhynchus mykiss, Toxicity, Biophysics, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Biomarkers, Water Pollutants, Chemical
Abstract: International audience; The present study was the first approach conducted under environmental concentrations of Gd-DOTA and Gd-DTPA-BMA to assess cellular impacts of these compounds. Gd-DOTA (Gadoteric acid) is one of the most stable contrast agent, currently used as Dotarem® formulation during Magnetic Resonance Imaging exams. The study was mainly performed on a Zebra Fish cell line (ZF4; ATCC CRL-2050). At the concentrations of 0.127 nM and 63.59 nM (respectively 20 ng and 10 μg of Gd/L), we did not observed any toxicity of Dotarem® but a slowdown of the cell growth was clearly measured. The effect is independent of medium renewing during 6 days of cell culturing. The same effect was observed i-with Gd-DOTA on another fish cell line (RT W1 gills; ATCC CRL-2523) and ii-with another contrast agent (Gd-DTPA-BMA - Omniscan®) on ZF4 cells. On the ZF4 cell line, the diminution of the cell growth was of the same order during 20 days of exposure to a culture medium spiked with 63.59 nM of Dotarem® and was reversible within the following 8 days when Dotarem® was removed from the medium. As shown by using modified DOTA structure (Zn-DOTA), the effect may be due to the chelating structure of the contrast agent rather than to the Gd ion. Until now, the main attention concerning the impact of Gd-CA on living cells concerned the hazard due to Gd release. According to our results, quantifying the presence of Gd-CA chelating structures in aquatic environments must be also monitored.
Published: 2019
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5. Nephrotoxic drug burden among 1001 critically ill patients: impact on acute kidney injury

Author: Laurent Martin-Lefevre, Bruno Giraudeau, Stephan Ehrmann, Dalila Benzekri-Lefevre, Jean-Claude Lacherade, Auguste Dargent, Julie Helms, Anne Bretagnol, Jean-Pierre Quenot, Arnaud Desachy, Ferhat Meziani, Elsa Tavernier, Pierre-François Dequin, René Robert, Jean-Etienne Herbrecht, Gaëtan Plantefève, Aurélie Joret, Frédéric Bellec, Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mitochondrie, stress oxydant et protection musculaire (MSP), Université de Strasbourg (UNISTRA), Centre d’Investigation Clinique [Tours] CIC 1415 (CIC ), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de biostatistique et information médicale de l’hôpital Saint Louis (Equipe ECSTRA) (SBIM), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut national du cancer [Boulogne] (INCA)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Réanimation Médicale (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional d'Orléans (CHRO), Université de La Réunion (UR), Service de réanimation et de surveillance continue [CH Mautauban], Centre hospitalier de Montauban, Centre Hospitalier Victor Dupouy, Service de Réanimation Polyvalente - CHR d’Orleans, Centre Hospitalier Départemental - Hôpital de La Roche-sur-Yon, CIC - Tours, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Soins Critiques [Bretonneau], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau-Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ehrmann, Stephan, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut national du cancer [Boulogne] (INCA)-Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre hospitalier de Montauban (CH Montauban), and Centre Hospitalier Départemental - Hôpital de La Roche-sur-Yon (CHD Vendée)
Subjects: medicine.medical_specialty, Intensive-care units [MeSH], Critical Care and Intensive Care Medicine, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, MESH: Vancomycin, 03 medical and health sciences, 0302 clinical medicine, MESH: Kidney tubular necrosis, Renal insufficiency [MeSH], Internal medicine, Anesthesiology, MESH: Renal insufficiency, medicine, MESH: Intensive-care units, Kidney tubular necrosis, MESH: Diuretics, 030212 general & internal medicine, Medical prescription, Simplified Acute Physiology Score, Prospective cohort study, MESH: Acute, business.industry, Research, Acute kidney injury, lcsh:Medical emergencies. Critical care. Intensive care. First aid, MESH: Aminoglycosides, 030208 emergency & critical care medicine, Odds ratio, lcsh:RC86-88.9, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, medicine.disease, Vancomycin [MeSH], [SDV.MHEP.UN] Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, Diuretics [MeSH], 3. Good health, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, Contrast media [MeSH], Cohort, MESH: Contrast media, Aminoglycosides [MeSH], business, Acute [MeSH], Kidney disease
Abstract: Background Nephrotoxic drug prescription may contribute to acute kidney injury (AKI) occurrence and worsening among critically ill patients and thus to associated morbidity and mortality. The objectives of this study were to describe nephrotoxic drug prescription in a large intensive-care unit cohort and, through a case–control study nested in the prospective cohort, to evaluate the link of nephrotoxic prescription burden with AKI. Results Six hundred and seventeen patients (62%) received at least one nephrotoxic drug, among which 303 (30%) received two or more. AKI was observed in 609 patients (61%). A total of 351 patients were considered as cases developing or worsening AKI a given index day during the first week in the intensive-care unit. Three hundred and twenty-seven pairs of cases and controls (patients not developing or worsening AKI during the first week in the intensive-care unit, alive the case index day) matched on age, chronic kidney disease, and simplified acute physiology score 2 were analyzed. The nephrotoxic burden prior to the index day was measured in drug.days: each drug and each day of therapy increasing the burden by 1 drug.day. This represents a semi-quantitative evaluation of drug exposure, potentially easy to implement by clinicians. Nephrotoxic burden was significantly higher among cases than controls: odds ratio 1.20 and 95% confidence interval 1.04–1.38. Sensitivity analysis showed that this association between nephrotoxic drug prescription in the intensive-care unit and AKI was predominant among the patients with lower severity of disease (simplified acute physiology score 2 below 48). Conclusions The frequently observed prescription of nephrotoxic drugs to critically ill patients may be evaluated semi-quantitatively through computing drug.day nephrotoxic burden, an index significantly associated with subsequent AKI occurrence, and worsening among patients with lower severity of disease.
Published: 2019
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6. Assessment of the targeting specificity of a fluorescent albumin conceived as a preclinical agent of the liver function

Author: Michel Bessodes, Pascal Houzé, Johanne Seguin, Katia Lemdani, Nathalie Mignet, Rabah Gahoual, Daniel Scherman, Hugo Salmon, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de pharmacologie chimique et génétique et d'imagerie (UPCGI - UMR 8151 / UMR_S 1022 ), Centre de recherche de Vitry-Alfortville, Rhone Poulenc SA, Laboratoire de Neurochimie, Hospices Civils de Lyon (HCL), Mignet, Nathalie, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5), Université Sorbonne Paris Cité (USPC), Université Paris sciences et lettres (PSL), Service de biochimie métabolique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Necker - Enfants Malades [AP-HP], Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP), Paris Sciences et Lettres (PSL), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], and PSL Research University (PSL)
Subjects: 0301 basic medicine, [SDV]Life Sciences [q-bio], Contrast Media, Lactose, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [SPI.MAT]Engineering Sciences [physics]/Materials, chemistry.chemical_compound, Mice, MESH: Liver Neoplasms, [CHIM] Chemical Sciences, General Materials Science, Tissue Distribution, MESH: Animals, Mice, Inbred BALB C, medicine.diagnostic_test, Liver Neoplasms, Optical Imaging, [CHIM.MATE]Chemical Sciences/Material chemistry, Carbocyanines, Human serum albumin, Fluorescence, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Biochemistry, Liver, Hepatocyte, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], Female, MESH: Carbocyanines, medicine.drug, MESH: Cell Line, Tumor, MESH: Mice, Inbred BALB C, MESH: Serum Albumin, Context (language use), [SDV.CAN]Life Sciences [q-bio]/Cancer, Flow cytometry, 03 medical and health sciences, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Cell Line, Tumor, MESH: Contrast Media, medicine, MESH: Transplantation, Homologous, Animals, Humans, Transplantation, Homologous, [CHIM]Chemical Sciences, MESH: Lactose, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], MESH: Tissue Distribution, MESH: Mice, Serum Albumin, MESH: Optical Imaging, MESH: Humans, Albumin, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 030104 developmental biology, chemistry, Liver function, MESH: Female, MESH: Liver
Abstract: International audience; In the context of increasing liver diseases, no contrast agent is currently available in Europe and the United States to directly assess the liver function. Only neolactosylated human serum albumin is being clinically used in Asia. In order to perform preclinical studies in the context of liver diseases, we conceived a fluorescent lactosylated albumin for the quantification of liver functional cells (l-Cyal). Precise characterization was achieved in order to determine the amounts of lactose and Cyanine 5 (Cy5) coupled to the albumin. In addition, potential aggregation was characterized by asymmetrical flow field-flow fractionation hyphenated to multi-angle light scattering (AF4-MALS). The optimal functionalized albumin exhibited a mass greater than 87 kDa which corresponds to the addition of 34 lactose moieties per protein and 1-2 Cy5 labels. Also, no significant formation of aggregates could be identified due to the modification of the native albumin. In healthy mice, the accumulation of l-Cyal in the liver and its selectivity for hepatocyte cells were shown by optical imaging and flow cytometry. Administration of l-Cyal to mice bearing liver metastases showed a reduced signal in the liver related to a decrease in the number of hepatocytes. The l-Cyal bioimaging contrast agent could be particularly useful for assessing the state of liver related diseases.
Published: 2018
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7. Ultra-small superparamagnetic iron oxide enhancement is associated with higher loss of brain tissue structure in clinically isolated syndrome

Author: Jean-Philippe Ranjeva, Gilles Edan, Laurent Pierot, Elise Bannier, Isabelle Berry, Jean Pelletier, Maxime Guye, Jean-Christophe Ferré, Christian Barillot, Adil Maarouf, Bertrand Audoin, Wafaa Zaaraoui, Ayman Tourbah, Arnaud Le Troter, Hôpital Maison Blanche, Centre Hospitalier Universitaire de Reims (CHU Reims), Département de Radiologie [CHU de Rennes], Université de Rennes (UR), Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Vision, Action et Gestion d'informations en Santé (VisAGeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE (IRISA-D5), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Service de Médecine Nucléaire - Pierre-Paul Riquet [CHU Toulouse], Pôle imagerie médicale [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université de Reims Champagne-Ardenne (URCA), Centre d'Exploration Métabolique par Résonance Magnétique [Marseille] (CEMEREM), Hôpital de la Timone [CHU - APHM] (TIMONE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Université de Rennes (UNIV-RENNES)-CentraleSupélec-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec, CHU Toulouse [Toulouse], Neurologie, maladies neuro-musculaires [Hôpital de la Timone - APHM], Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Service de Neurologie [Rennes], Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Service de Médecine Nucléaire [Toulouse], and Edan, Gilles
Subjects: Male, Pathology, Time Factors, Gadolinium, Axonal loss, Contrast Media, MESH: Nerve Degeneration, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, Medicine, Longitudinal Studies, Prospective Studies, MESH: Dextrans, Magnetite Nanoparticles, MESH: Longitudinal Studies, ComputingMilieux_MISCELLANEOUS, Clinically isolated syndrome, medicine.diagnostic_test, Brain, MESH: Macrophage Activation, Dextrans, Prognosis, Magnetic Resonance Imaging, MESH: Predictive Value of Tests, Neurology, MESH: Young Adult, Disease Progression, Female, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Disease Progression, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], France, Infiltration (medical), MRI, Adult, medicine.medical_specialty, MESH: Demyelinating Diseases, chemistry.chemical_element, MESH: Magnetite Nanoparticles, macrophage, MESH: Prognosis, Multiple sclerosis, 03 medical and health sciences, Young Adult, MESH: Brain, In vivo, Predictive Value of Tests, MESH: Contrast Media, Humans, Magnetization transfer, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Humans, business.industry, Macrophages, MESH: Time Factors, MESH: Macrophages, Magnetic resonance imaging, MESH: Adult, Macrophage Activation, medicine.disease, USPIO, MESH: Male, MESH: Prospective Studies, MESH: France, chemistry, clinically isolated syndrome, Nerve Degeneration, Neurology (clinical), business, MESH: Female, 030217 neurology & neurosurgery, Demyelinating Diseases
Abstract: Background: Macrophages are important components of inflammatory processes in multiple sclerosis, closely linked to axonal loss, and can now be observed in vivo using ultra-small superparamagnetic iron oxide (USPIO). In the present 1-year longitudinal study, we aimed to determine the prevalence and the impact on tissue injury of macrophage infiltration in patients after the first clinical event of multiple sclerosis. Methods: Thirty-five patients, 32 years mean age, were imaged in a mean of 66 days after their first event using conventional magnetic resonance imaging, gadolinium (Gd) to probe blood–brain barrier integrity, USPIO to study macrophage infiltration and magnetization transfer ratio (MTR) to assess tissue structure integrity. Statistics were performed using two-group repeated-measures ANOVA. Any patient received treatment at baseline. Results: At baseline, patients showed 17 USPIO-positive lesions reflecting infiltration of macrophages present from the onset. This infiltration was associated with local higher loss of tissue structure as emphasized by significant lower MTRnorm values ( p+/Gd+ lesions ( n=16; MTRnormUSPIO+/Gd+=0.78 at baseline, MTRnormUSPIO+/Gd+=0.81 at M12) relative to USPIO-/Gd+ lesions ( n=67; MTRnormUSPIO-/Gd+=0.82 at baseline, MTRnormUSPIO–/Gd+=0.85 at M12). No interaction in MTR values was observed during the 12 months follow-up (lesion type × time). Conclusion: Infiltration of activated macrophages evidenced by USPIO enhancement, is present at the onset of multiple sclerosis and is associated with higher and persistent local loss of tissue structure. Macrophage infiltration affects more tissue structure while tissue recovery during the following year has a similar pattern for USPIO and Gd-enhanced lesions, leading to relative higher persistent local loss of tissue structure in lesions showing USPIO enhancement at baseline.
Published: 2016
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8. Temporal deformation pattern in acute and late phases of ST-elevation myocardial infarction: incremental value of longitudinal post-systolic strain to assess myocardial viability

Author: Yves Juillière, Patrick Rossignol, Nicolas Girerd, Erwan Bozec, Emilien Micard, Maxime Benichou, Pierre-Yves Marie, Christine Selton-Suty, Nicolas Sadoul, Damien Mandry, Marine Beaumont, Simon Lemoine, Kevin Duarte, Olivier Huttin, Service de Cardiologie [Institut Lorrain du Cœur et des Vaisseaux], Institut Lorrain du Coeur et des Vaisseaux Louis Mathieu [Nancy], Service de Médecine Nucléaire [Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre d'investigation clinique plurithématique Pierre Drouin [Nancy] (CIC-P), Centre d'investigation clinique [Nancy] (CIC), Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Adaptative Diagnostique et Interventionnelle (IADI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), and Université de Lorraine (UL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, Time Factors, MESH: Myocardial Contraction, Contrast Media, Speckle tracking echocardiography, 030204 cardiovascular system & hematology, MESH: Observer Variation, MESH: Ventricular Function, Left, Ventricular Function, Left, Strain, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, Systolic strain, Cutoff, Myocardial infarction, Prospective Studies, Prospective cohort study, MESH: Aged, Observer Variation, MESH: Stress, Mechanical, MESH: Middle Aged, medicine.diagnostic_test, Left ventricular function, MESH: Magnetic Resonance Imaging, Cine, General Medicine, Middle Aged, Prognosis, MESH: Predictive Value of Tests, Biomechanical Phenomena, MESH: Reproducibility of Results, Echocardiography, Predictive value of tests, Cardiology, Female, France, Cardiology and Cardiovascular Medicine, medicine.medical_specialty, MESH: Myocardium, MESH: Biomechanical Phenomena, Magnetic Resonance Imaging, Cine, MESH: Prognosis, 03 medical and health sciences, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Cardiac magnetic resonance imaging, St elevation myocardial infarction, Predictive Value of Tests, MESH: Contrast Media, Internal medicine, medicine, Humans, MESH: Tissue Survival, MESH: ST Elevation Myocardial Infarction, Aged, Tissue Survival, MESH: Humans, business.industry, Myocardium, MESH: Time Factors, Reproducibility of Results, medicine.disease, Myocardial Contraction, MESH: Prospective Studies, MESH: Male, MESH: France, Deformation mechanics, ST Elevation Myocardial Infarction, MESH: Echocardiography, Stress, Mechanical, business, MESH: Female
Abstract: International audience; BACKGROUND:Identification of transmural extent and degree of non-viability after ST-segment elevation myocardial infarction (STEMI) is clinically important. The objective of the present study was to assess the regional mechanics and temporal deformation patterns using speckle tracking echocardiography (STE) in acute and later phases of STEMI to predict myocardial damage in these patients.METHODS AND RESULTS:Ninety-eight patients with first STEMI underwent both echocardiography and cardiac magnetic resonance imaging in acute phase and at 6 months follow-up with 2D STE-derived measurements of peak longitudinal strain (PLS), Pre-STretch index (PST) and post-systolic deformation index (PSI). For each segment, late gadolinium enhancement (LGE) was defined as transmural (LGE >66 %) or non-transmural (
Published: 2016
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9. Advanced Techniques Using Contrast Media in Neuroimaging

Author: Meng Law, Jean-Christophe Ferré, Mark S. Shiroishi, Department of Radiology, Keck School of Medicine [Los Angeles], University of Southern California (USC)-University of Southern California (USC), Service de radiologie et imagerie médicale [Rennes], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Service de radiologie et imagerie médicale [Rennes] = Radiology [Rennes], CHU Pontchaillou [Rennes], and Ferré, Jean-Christophe
Subjects: medicine.medical_specialty, MESH: Brain Diseases, Brain tumor, Contrast Media, Gadolinium, Article, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Neuroimaging, MESH: Contrast Media, Humans, Medicine, Dementia, Image acquisition, Radiology, Nuclear Medicine and imaging, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Brain Diseases, MESH: Humans, medicine.diagnostic_test, business.industry, MESH: Neuroradiography, Multiple sclerosis, Magnetic resonance imaging, Image Enhancement, medicine.disease, Magnetic Resonance Imaging, 3. Good health, Neuroradiography, Dynamic contrast-enhanced MRI, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Radiology, MESH: Image Enhancement, MESH: Gadolinium, business, 030217 neurology & neurosurgery, Dynamic susceptibility
Abstract: International audience; This article presents an overview of advanced magnetic resonance (MR) imaging techniques using contrast media in neuroimaging, focusing on T2*-weighted dynamic susceptibility contrast MR imaging and T1-weighted dynamic contrast-enhanced MR imaging. Image acquisition and data processing methods and their clinical application in brain tumors, stroke, dementia, and multiple sclerosis are discussed.
Published: 2012
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10. Localized hypertrophic neuropathy of the sciatic nerve in children: MRI findings

Author: Franck Marin, Bertrand Bruneau, Philippe Violas, Laurent Riffaud, Yves Gandon, Anne Michel, Jean-Yves Gauvrit, Adrien Roux, Catherine Tréguier, Service de radiologie et imagerie médicale [Rennes] = Radiology [Rennes], CHU Pontchaillou [Rennes], Service de neurochirurgie [Rennes] = Neurosurgery [Rennes], Service de chirurgie pédiatrique [Rennes] = Paediatric / Pediatric surgery [Rennes], Service d'explorations fonctionnelles neurologiques [Rennes], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CHU Pontchaillou [Rennes], Vision, Action et Gestion d'informations en Santé (VisAGeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE (IRISA-D5), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Rennes (UR)-CHU Pontchaillou [Rennes], Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), and Gauvrit, Jean-Yves
Subjects: Pathology, intraneural perineuromia, Contrast Media, sciatic nerve, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, MESH: Aged, 80 and over, 0302 clinical medicine, MESH: Risk Factors, MESH: Carotid Stenosis, Hypertrophic neuropathy, MESH: Child, Medicine, Neuroradiology, Localized hypertrophic neuropathy, MESH: Aged, child, MESH: Middle Aged, MESH: Hypertrophy, Peripheral Nervous System Diseases, Magnetic Resonance Imaging, MESH: Infant, MESH: Predictive Value of Tests, Intraneural perineurioma, MESH: Leg, 3. Good health, MESH: Sciatic Nerve, Child, Preschool, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Peripheral Nervous System Diseases, Sciatic nerve, MESH: Hemorrhage, Mri findings, medicine.medical_specialty, MESH: Imaging, Three-Dimensional, MESH: Stroke, MESH: Multivariate Analysis, MESH: Electromyography, 03 medical and health sciences, Imaging, Three-Dimensional, MESH: Contrast Media, Humans, Radiology, Nuclear Medicine and imaging, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Retrospective Studies, Neurological deficit, Leg, MESH: Humans, Electromyography, business.industry, MESH: Child, Preschool, Infant, MESH: Retrospective Studies, Hypertrophy, MESH: Prospective Studies, MESH: Male, eye diseases, nervous system, Pediatrics, Perinatology and Child Health, business, MESH: Female, 030217 neurology & neurosurgery
Abstract: International audience; BACKGROUND: Localized hypertrophic neuropathy (LHN) of the sciatic nerve in children is a rare condition characterized by a painless neurological deficit in the sciatic nerve territory. OBJECTIVE: To demonstrate the role of MRI using a specific protocol and describe the primary findings in LHN. MATERIALS AND METHODS: Imaging in four children (age 2 years to 12 years) is presented. All children presented with lower limb asymmetry. Three had a steppage gait. LHN was confirmed by electrophysiological studies and by MRI of the whole sciatic nerve with a dedicated protocol covering the lumbar spine and the lower limb. RESULTS: There were four direct MRI findings: (1) linear and focal hypertrophy with progressive enlargement of a peripheral nerve or plexus diameter, (2) abnormal hyperintensity of the nerve on T2-weighted images, (3) preserved fascicular configuration, and (4) variable enhancement after intravenous gadolinium administration. In addition there were atrophy and fatty infiltration of innervated muscles. MRI was helpful for determining the extent of lesions and in excluding peripheral nerve compression or tumour. CONCLUSION: MRI of the whole sciatic nerve is the method of choice for diagnosing LHN of the sciatic nerve.
Published: 2012
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11. Diagnostic contributions of cardiac magnetic resonance imaging in patients presenting with elevated troponin, acute chest pain syndrome and unobstructed coronary arteries

Author: Dominique Boulmier, Pierre-Axel Lentz, Marc Bedossa, Hervé Le Breton, Guillaume Leurent, B. Langella, A. Larralde, Claire Fougerou, Service de cardiologie et maladies vasculaires [Rennes] = Cardiac, Thoracic, and Vascular Surgery [Rennes], CHU Pontchaillou [Rennes], Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), CIC-IT Rennes, Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de radiologie et imagerie médicale [Rennes] = Radiology [Rennes], Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, Myocardite, MESH: Chi-Square Distribution, Cardiac magnetic resonance, Myocardial Infarction, Contrast Media, MESH: Takotsubo Cardiomyopathy, 030204 cardiovascular system & hematology, Coronary Angiography, Chest pain, Coronary artery disease, 0302 clinical medicine, MESH: Up-Regulation, MESH: Coronary Vessels, MESH: Syndrome, Prospective Studies, 030212 general & internal medicine, Myocardial infarction, MESH: Myocardial Perfusion Imaging, Ejection fraction, biology, medicine.diagnostic_test, MESH: Chest Pain, Syndrome de takotsubo, Myocardial Perfusion Imaging, MESH: Magnetic Resonance Imaging, Cine, Syndrome, General Medicine, Syndrome coronaire aigu, Coronary Vessels, MESH: Predictive Value of Tests, Troponin, Up-Regulation, 3. Good health, MESH: Myocardial Infarction, Myocarditis, MESH: Young Adult, Acute Disease, cardiovascular system, Cardiology, MESH: Acute Disease, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Female, Acute coronary syndrome, France, Radiology, medicine.symptom, Takotsubo syndrome, Cardiology and Cardiovascular Medicine, Adult, Chest Pain, medicine.medical_specialty, Magnetic Resonance Imaging, Cine, Infarctus du myocarde, MESH: Meglumine, Young Adult, 03 medical and health sciences, Meglumine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, MESH: Myocarditis, Predictive Value of Tests, Takotsubo Cardiomyopathy, Cardiac magnetic resonance imaging, MESH: Contrast Media, Internal medicine, Organometallic Compounds, medicine, Humans, MESH: Humans, Chi-Square Distribution, business.industry, MESH: Biological Markers, MESH: Adult, MESH: Organometallic Compounds, medicine.disease, MESH: Male, MESH: Prospective Studies, MESH: Coronary Angiography, MESH: France, IRM cardiaque, biology.protein, MESH: Troponin, Myocardial infarction diagnosis, business, human activities, MESH: Female, Biomarkers
Abstract: International audience; AIMS: Myocardial infarction with unobstructed coronary artery disease represents a serious diagnostic challenge. The role of cardiac magnetic resonance in the management of cardiomyopathies is increasing. We examined the diagnostic contributions of cardiac magnetic resonance in patients presenting with acute chest pain syndrome, elevated serum cardiac troponin concentrations and no significant coronary artery stenoses. METHODS: Over a 3-year period, 107 consecutive patients (mean age 43.5 years; 62% men) presented to our institution with acute onset of chest pain, elevated serum troponin concentration and unobstructed coronary arteries, and underwent 3-tesla cardiac magnetic resonance at a mean delay of 6.9 days. A diagnosis was made based on: wall motion abnormalities and pericardial effusion on cine mode; myocardial oedema on T2-weighted imaging; abnormalities on first-pass perfusion imaging; and late gadolinium enhancement on T1-weighted imaging. RESULTS: Cardiac magnetic resonance was normal in 10.3% of patients and contributed a diagnosis in 89.7%, including myocarditis in 59.9%, stress cardiomyopathy (takotsubo syndrome) in 14% and myocardial infarction in 15.8%. Patients with normal cardiac magnetic resonance had a significantly lower mean peak troponin concentration (2.6ng/mL) than patients with diagnostic cardiac magnetic resonance (9.7ng/mL; P=0.01). CONCLUSION: Cardiac magnetic resonance contributed a diagnosis in nearly 90% of patients presenting with acute chest pain, elevated serum troponin and unobstructed coronary arteries.
Published: 2011
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12. Liver methylene fraction by dual- and triple-echo gradient-echo imaging at 3.0T: Correlation with proton MR spectroscopy and estimation of robustness after SPIO administration

Author: David Masson, Patrick Hillon, Isabelle Robin, Romaric Loffroy, Boris Guiu, Serge Aho, Jean-Michel Petit, Bruno Vergès, Jean-Pierre Cercueil, Douraied Ben Salem, Denis Krausé, Service de radiologie et d'Imagerie médicale diagnostique et thérapeutique (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Unité de Génétique Moléculaire Animale (UGMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Service d'Ophtalmologie (CHU de Dijon), Laboratoire Electronique, Informatique et Image [UMR6306] (Le2i), Université de Bourgogne (UB)-École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Service de Diabétologie, Service d'Hépato-Gastroentérologie, Hôpital du Bocage, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Unité de Génétique Moléculaire Animale (UMR GMA), Université de Brest (UBO)-Université de Brest (UBO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Service d'Endocrinologie, Diabétologie et Maladies Métaboliques (CHU de Dijon), Service d'hépato-gastroentérologie et cancérologie digestive (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Unité de Génétique Moléculaire Animale ( UGMA ), Université de Limoges ( UNILIM ) -Institut National de la Recherche Agronomique ( INRA ), Laboratoire Electronique, Informatique et Image ( Le2i ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Groupe d'Etude de la Thrombose de Bretagne Occidentale ( GETBO ), Université de Brest ( UBO ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ) -Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon )
Subjects: Male, Magnetic Resonance Spectroscopy, MESH : Fatty Liver, MESH: Echo-Planar Imaging, [SDV]Life Sciences [q-bio], Carbon Compounds, Inorganic, MESH : Statistics as Topic, Statistics as Topic, MESH : Aged, Contrast Media, MESH : Carbon Compounds, Inorganic, MESH : Tissue Distribution, 030218 nuclear medicine & medical imaging, Correlation, chemistry.chemical_compound, 0302 clinical medicine, MESH : Dextrans, Non-alcoholic Fatty Liver Disease, MESH : Female, Tissue Distribution, MESH: Dextrans, Methylene, Magnetite Nanoparticles, MESH: Fatty Liver, MESH: Aged, MESH: Middle Aged, medicine.diagnostic_test, Echo-Planar Imaging, Dextrans, Nuclear magnetic resonance spectroscopy, MESH : Adult, Middle Aged, MESH: Reproducibility of Results, Adipose Tissue, Liver, Female, 030211 gastroenterology & hepatology, MESH : Sensitivity and Specificity, Protons, MESH: Adipose Tissue, Adult, Iron Overload, MESH : Male, MESH: Magnetite Nanoparticles, MESH : Adipose Tissue, Sensitivity and Specificity, MESH: Iron Overload, 03 medical and health sciences, Flip angle, Robustness (computer science), MESH: Contrast Media, Linear regression, medicine, MESH : Protons, Humans, MESH : Middle Aged, Radiology, Nuclear Medicine and imaging, MESH: Tissue Distribution, MESH: Statistics as Topic, Aged, MESH : Contrast Media, MESH : Iron Overload, MESH: Humans, MESH : Echo-Planar Imaging, [ SDV ] Life Sciences [q-bio], MESH: Magnetic Resonance Spectroscopy, business.industry, MESH : Reproducibility of Results, MESH : Humans, MESH: Biological Markers, MESH: Carbon Compounds, Inorganic, MESH : Liver, MESH : Magnetite Nanoparticles, Reproducibility of Results, MESH: Adult, Magnetic resonance imaging, MESH: Male, MESH: Sensitivity and Specificity, Proton mr spectroscopy, MESH : Biological Markers, Fatty Liver, chemistry, MESH : Magnetic Resonance Spectroscopy, MESH: Protons, Nuclear medicine, business, MESH: Female, Biomarkers, MESH: Liver
Abstract: Purpose To assess the systematic errors in liver methylene fraction (LMF) resulting from fat–fat interference effects with dual- and triple-echo gradient-recalled-echo Dual/Triple GRE) sequences and to test the robustness of these sequences after iron overloading. Materials and Methods Forty type-2 diabetic patients underwent LMF measurement by 3.0T 1H magnetic resonance spectroscopy (corrected for T1 and T2 decays) as the reference standard and liver fat fraction (%Fat) measurement by four Dual/Triple GRE sequences with 20° and 60° flip angle (α), corrected for T1 recovery. The same four sequences were repeated in eight patients after ferumoxide injection. Corrections for systematic errors were determined from the linear regressions (spectroscopy LMF values over Dual/Triple GRE %Fat values). Robustness was tested using Wilcoxon's signed-rank test. Results Fat–fat interference effects produced a ∼10% relative systematic error and T2* decay produced a 1.9%–4.2% absolute systematic error in LMF. When comparing before and after ferumoxide, dual-echo imaging with α = 20° and α = 60°, even when corrected, showed absolute differences of 7.23% [2.81%–10.25%] (P = 0.0117) and 5.65% [1.89%–8.216.8%] (P = 0.0117), respectively; compared to only 1.17% [0.08%–2.83%] (P = 0.0251) and 1.15% [0.37%–2.73%] (P = 0.2626) with triple-echo imaging and α = 20° and α = 60°, respectively. Conclusion Triple-echo imaging with α = 60° corrected for both T1 recovery and fat–fat interference effects is robust after superparamagnetic iron oxide (SPIO) administration and can reliably quantify LMF. J. Magn. Reson. Imaging 2011;33:119–127. © 2010 Wiley-Liss, Inc.
Published: 2010
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13. Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles: In vitro characterization and migration potential in rat brain

Author: Jean Jacques Le Jeune, Laurent Lemaire, Matthieu Jacquart, Claudia N. Montero-Menei, Florence Franconi, Gaëtan J.-R. Delcroix, Laurence Sindji, Ingénierie de la vectorisation particulaire, Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateforme d'Ingénierie et d'Analyses Moléculaires (PIAM), Université d'Angers (UA), Région Pays de la Loire, and Lemaire, Laurent
Subjects: cell migration, MESH: Etidronic Acid, Rostral migratory stream, MESH: Neurons, Contrast Media, MESH: Rats, Sprague-Dawley, MESH: Magnetic Resonance Imaging, Rats, Sprague-Dawley, 0302 clinical medicine, Cell Movement, MESH: Animals, Coloring Agents, MESH: Cell Movement, Cells, Cultured, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Cerebral Cortex, Neurons, MESH: Iron, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, 0303 health sciences, General Neuroscience, subventricular zone, Brain, Cell Differentiation, Etidronic Acid, Oxides, Cell migration, Magnetic Resonance Imaging, Olfactory Bulb, Neural stem cell, Cell biology, medicine.anatomical_structure, MESH: Cell Survival, SPIO, Stem cell, MESH: Coloring Agents, MESH: Cells, Cultured, MESH: Olfactory Bulb, MESH: Cell Differentiation, MESH: Rats, Cell Survival, Iron, Subventricular zone, Biology, Mesenchymal Stem Cell Transplantation, MESH: Brain, 03 medical and health sciences, In vivo, MESH: Contrast Media, medicine, Animals, MESH: Mesenchymal Stem Cell Transplantation, Molecular Biology, 030304 developmental biology, Mesenchymal stem cell, MESH: Embryo, Mammalian, Mesenchymal Stem Cells, Embryo, Mammalian, MESH: Cerebral Cortex, Rats, Olfactory bulb, MESH: Mesenchymal Stem Cells, MESH: Oxides, Nanoparticles, Neurology (clinical), Neuroscience, MESH: Nanoparticles, 030217 neurology & neurosurgery, Developmental Biology
Abstract: International audience; Mesenchymal stem cells (MSC) may transdifferentiate into neural cells in vitro under the influence of matrix molecules and growth factors present in neurogenic niches. However, further experiments on the behavior of such stem cells remain to be done in vivo. In this study, rat MSC (rMSC) have been grafted in a neurogenic environment of the rat brain, the subventricular zone (SVZ), in order to detect and follow their migration using superparamagnetic iron oxide (SPIO) nanoparticles. We sought to characterize the potential effect of iron loading on the behavior of rMSC as well as to address the potential of rMSC to migrate when exposed to the adequate brain microenvironment. 1-hydroxyethylidene-1.1-bisphosphonic acid (HEDP)-coated SPIO nanoparticles efficiently labeled rMSC without significant adverse effects on cell viability and on the in vitro differentiation potential. In opposition to iron-labeled rat neural stem cells (rNSC), used as a positive control, iron-labeled rMSC did not respond to the SVZ microenvironment in vivo and did not migrate, unless a mechanical lesion of the olfactory bulb was performed. This confirmed the known potential of iron-labeled rMSC to migrate toward lesions and, as far as we know, this is the first study describing such a long distance migration from the SVZ toward the olfactory bulb through the rostral migratory stream (RMS).
Published: 2009
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14. QUANTITATIVE FUNCTIONAL IMAGING AND KINETIC STUDIES WITH HIGH-Z CONTRAST AGENTS USING SYNCHROTRON RADIATION COMPUTED TOMOGRAPHY

Author: Pekka Suortti, Liisa Porra, Sam Bayat, Hélène Elleaume, Jean-François Adam, François Estève, European Synchrotron Radiation Facility (ESRF), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie, Université de Picardie Jules Verne (UPJV), Department of Physics [Helsinki], Falculty of Science [Helsinki], University of Helsinki-University of Helsinki, Département MRI, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Serduc, Raphael, and Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki
Subjects: Accuracy and precision, MESH: Rats, Physiology, Contrast Media, Perfusion scanning, Ventilation/perfusion ratio, 030218 nuclear medicine & medical imaging, MESH: Brain, Mice, 03 medical and health sciences, 0302 clinical medicine, MESH: Contrast Media, Physiology (medical), Animals, Medicine, MESH: Animals, MESH: Lung, MESH: Mice, Lung, Pharmacology, business.industry, Subtraction, Brain, Blood flow, Rats, Functional imaging, Cerebral blood flow, MESH: Synchrotrons, MESH: Tomography, X-Ray Computed, Tomography, X-Ray Computed, business, Nuclear medicine, Perfusion, Synchrotrons, 030217 neurology & neurosurgery
Abstract: International audience; 1. There is an increasing demand in diagnostic radiology for extracting additional morphological and functional quantitative parameters from three-dimensional computed tomography (CT) images. Synchrotron radiation computed tomography (SRCT) is the state-of-the-art method in preclinical X-ray CT, because its performance is close to the theoretical limits in terms of accuracy and precision. 2. The SRCT method with monochromatic X-ray beams yields absolute high-Z element contrast agent concentrations, without errors arising from beam hardening or scatter artefacts, by using digital subtraction techniques of the sinograms. Each pixel of the reconstructed difference images provides a quantitative concentration versus time curve of inhaled or injected high-Z contrast agents (xenon or iodine) with a high sensitivity. This is the key point of two functional imaging techniques that were developed at the European Synchrotron Radiation Facility: brain perfusion and lung function (ventilation and perfusion). 3. These two imaging techniques provide parametric images expressed in absolute perfusion parameters (blood volume, blood flow, mean transit time and capillary permeability) or ventilation parameters (lung volume, regional lung ventilation, bronchial lumen size, regional airway and lung compliance) with a high accuracy and precision. 4. The aim of the present brief review is to give a snapshot of the status and perspectives of these two imaging techniques, with emphasis on the performances and interests for functional imaging. Two separate sections will then describe the results obtained so far using SRCT as an in vivo functional imaging tool for measuring changes in haemodynamics and ventilation, in the investigation of experimental pathophysiology and in the effects of therapeutic intervention.
Published: 2009
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15. Contrast-Enhanced Ultrasound and Prostate Cancer; A Multicentre European Research Coordination Project

Author: Michael Mitterberger, Ferdinand Frauscher, Chris J Harvey, Margot H. Wink, Olivier Rouvière, Leo Palwein, Jean J.M.C.H. de la Rosette, Jean-Yves Chapelon, Hessel Wijkstra, David O. Cosgrove, Inst Pharm & Mol Biotechnol, Universität Heidelberg [Heidelberg], Applications des ultrasons à la thérapie, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'étude spatiale des rayonnements (CESR), Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Signal Processing Systems, and Biomedical Diagnostics Lab
Subjects: Male, medicine.medical_specialty, Biopsy, Urology, 030232 urology & nephrology, Contrast Media, Context (language use), SDG 3 – Goede gezondheid en welzijn, Endosonography, MESH: Biopsy, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, SDG 3 - Good Health and Well-being, Prostate, MESH: Contrast Media, medicine, Humans, Neoplasm Staging, MESH: Endosonography, MESH: Humans, business.industry, Ultrasound, Prostatic Neoplasms, Reproducibility of Results, Cancer, Ultrasonography, Doppler, MESH: Neoplasm Staging, medicine.disease, MESH: Male, 3. Good health, Surgery, MESH: Reproducibility of Results, Europe, medicine.anatomical_structure, MESH: Prostatic Neoplasms, 030220 oncology & carcinogenesis, MESH: Ultrasonography, Doppler, Microbubbles, Hormonal therapy, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Europe, Radiology, business, Contrast-enhanced ultrasound
Abstract: Context: Contrast-enhanced ultrasound is a real-time imaging technique with the capability of visualizing perfusion patterns. Since tumour growth is associated with changes in vascularisation, this modality is under research for imaging of various tumour types. Studies have shown promising results for the diagnosis of prostate cancer for various imaging techniques; however, the exact value of each technique is still unclear. Objective: To determine the value of contrast-enhanced ultrasound (CEUS) in the detection, localisation, and follow-up of treatment for prostate cancer. Evidence acquisition: In the period 2002-2006, research in four European centres regarding CEUS of the prostate was coordinated in a combined program. This paper describes and combines the results of these studies. Evidence synthesis: Various techniques were developed and researched during the period of this program. Studies showed that prostate cancer could be visualized and localized in up to 78%. Visualization of the tumour enabled better detection; targeted biopsies lead to fewer biopsies per session without loss of detection rate. A combined approach offered the highest detection rate. CEUS could be used to visualize the effects of high-intensity focussed ultrasound and hormonal therapy for prostate cancer with success, and identified patients with an early relapse. Unfortunately, pretreatment evaluation could not identify the nonresponders beforehand. Conclusions: This research project was a first step towards routine use of CEUS in the clinical detection and follow-up of prostate cancer; and new combined studies are initiated. (C) 2008 European Association of Urology. Published by Elsevier B.V. All rights reserved
Published: 2008
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16. Nonspherical Oscillations of Ultrasound Contrast Agent Microbubbles

Author: Valeria Garbin, Benjamin Dollet, Sander van der Meer, Michel Versluis, Nico de Jong, Detlef Lohse, Institut de Physique de Rennes (IPR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Laboratorio Nazionale TASC (CNR-INFM), Istituto Nazionale per la Fisica della Materia, Institute for Biomedical Technology (MIRA), University of Twente [Netherlands], ErasmusMC- ThoraxCentruum, Physics of Fluids, Faculty of Science and Technology, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), and University of Twente
Subjects: Materials science, Acoustics and Ultrasonics, Surface Properties, Ultrasonic Therapy, Bubble, Biophysics, Contrast Media, Subharmonics, Nonspherical oscillations, 01 natural sciences, 010305 fluids & plasmas, Physics::Fluid Dynamics, Optics, MESH: Rheology, MESH: Contrast Media, 0103 physical sciences, Pressure, Scattering, Radiation, Radiology, Nuclear Medicine and imaging, MESH: Scattering, Radiation, Sound pressure, 010301 acoustics, Phospholipids, MESH: Phospholipids, MESH: Surface Properties, High-speed imaging, Microbubbles, Radiological and Ultrasound Technology, business.industry, Resonance, Radius, Mechanics, MESH: Ultrasonic Therapy, Ultrasound contrast agents, Image Enhancement, Amplitude, Optical tweezers, 2023 OA procedure, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Image Enhancement, Rheology, MESH: Pressure, business, MESH: Microbubbles, Dimensionless quantity
Abstract: International audience; The occurrence of nonspherical oscillations (or surface modes) of coated microbubbles, used as ultrasound contrast agents in medical imaging, is investigated using ultra-high-speed optical imaging. Optical tweezers designed to micromanipulate single bubbles in 3-D are used to trap the bubbles far from any boundary, enabling a controlled study of the nonspherical oscillations of free-floating bubbles. Nonspherical oscillations appear as a parametric instability and display subharmonic behavior: they oscillate at half the forcing frequency, which was fixed at 1.7 MHz in this study. Surface modes are shown to preferentially develop for a bubble radius near the resonance of radial oscillations. In the studied range of acoustic pressures, the growth of surface modes saturates at a level far below bubble breakage. With the definition of a single, dimensionless deformation parameter, the amplitude of nonspherical deformation is quantified as a function of the bubble radius (between 1.5 and 5 microm) and of the acoustic pressure (up to 200 kPa).
Published: 2008
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17. Cerebral Blood Volume Quantification in a C6 Tumor Model Using Gadolinium per (3,6-Anhydro) α-Cyclodextrin as a New Magnetic Resonance Imaging Preclinical Contrast Agent

Author: Jean-François Le Bas, Soâd Aous, Hana Lahrech, Adriana-Teodora Perles-Barbacaru, Andrée Gadelle, Jean-Claude Debouzy, Pascal H. Fries, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherches du Service de Santé des Armées (CRSSA), Service de Santé des Armées, RMN biomédicale : de la cellule à l'homme (RBCH), Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-DIR CENTRALE DU SSA-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Reconnaissance Ionique et Chimie de Coordination (RICC), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), This work was supported by the Canceropôle Lyon Auvergne Rhône-Alpes (CLARA), the EC COST Action D-38 Metal-Based Systems for Molecular Imaging Applications and the European Molecular Imaging Laboratories (EMIL) network, the Nuclear Energy Division of the Atomic Energy Commission (CEA), the GdR PARIS, the Association for the Research on Cancer (ARC) for postgraduate research grant., Collaboration, Issartel, Jean-Paul, Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: MESH: Models, Cardiovascular, MESH: Blood Volume, Magnetic Resonance Spectroscopy, Gadolinium, Contrast Media, Blood volume, blood–brain barrier, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, MESH: Blood-Brain Barrier, MESH: Glioma, Mice, 0302 clinical medicine, MESH: Animals, Blood Volume, medicine.diagnostic_test, Brain Neoplasms, MESH: alpha-Cyclodextrins, Models, Cardiovascular, cerebral blood volume, MESH: Cerebrovascular Circulation, Glioma, contrast agent, Magnetic Resonance Imaging, Extravasation, medicine.anatomical_structure, Neurology, Blood-Brain Barrier, relaxivity, Cerebrovascular Circulation, MESH: Brain Neoplasms, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Cardiology and Cardiovascular Medicine, brain tumor, alpha-Cyclodextrins, Brain tumor, chemistry.chemical_element, Mice, Inbred Strains, Blood–brain barrier, MESH: Mice, Inbred Strains, Nephrotoxicity, 03 medical and health sciences, In vivo, MESH: Contrast Media, Organometallic Compounds, medicine, Animals, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Mice, MESH: Magnetic Resonance Spectroscopy, business.industry, MESH: Organometallic Compounds, Magnetic resonance imaging, medicine.disease, cyclodextrin, chemistry, Neurology (clinical), MESH: Gadolinium, Nuclear medicine, business, 030217 neurology & neurosurgery
Abstract: In magnetic resonance imaging (MRI), cerebral blood volume (CBV) quantification is dependent on the MRI sequence and on the properties of the contrast agents (CAs). By using the rapid steady-state T1 method, we show the potential of gadolinium per (3,6-anhydro) α-cyclodextrin (Gd-ACX), a new MRI paramagnetic CA (inclusion complex of Gd3+ with per (3,6-anhydro)-α-cyclodextrin), for the CBV quantification in the presence of blood—brain barrier lesions. After biocompatibility and relaxivity experiments, in vivo experiments on rats were performed on a C6 tumor model with 0.05 mmol Gd-ACX/kg (1-weighted images, a signal enhancement of 170% appeared in vessels after injection, but not in the tumor (during the 1 h of observation), in contrast to the 90% signal enhancement obtained with Gd-DOTA (a clinical MRI CA) injected at a T1 isoefficient dose. This result shows the absence of Gd-ACX extravasation into the tumor tissue and its confinement to the vascular space. Fractional CBV values were found similar to Gd-ACX and Gd-DOTA in healthy brain tissue and in the contralateral hemisphere of tumor-bearing rats, whereas only Gd-ACX was appropriate for CBV quantification in tumor regions.
Published: 2008
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18. Radiobiological features of the anti-cancer strategies involving synchrotron X-rays

Author: Zuzana Bencokova, Jacques Balosso, Nicolas Foray, Serduc, Raphael, Rayonnement Synchrotron et Recherche Medicale (RSRM), European Synchrotron Radiation Facility (ESRF)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de cancérologie et radiothérapie, CHU Grenoble, and Université Joseph Fourier - Grenoble 1 (UJF)-European Synchrotron Radiation Facility (ESRF)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Nuclear and High Energy Physics, medicine.medical_specialty, Radiobiology, MESH: Rats, medicine.medical_treatment, MESH: Photosensitizing Agents, Contrast Media, High radiation, Synchrotron radiation, [SDV.CAN]Life Sciences [q-bio]/Cancer, Context (language use), 030218 nuclear medicine & medical imaging, law.invention, MESH: Glioma, 03 medical and health sciences, 0302 clinical medicine, Optics, [SDV.CAN] Life Sciences [q-bio]/Cancer, Microbeam radiation therapy, law, MESH: Contrast Media, medicine, Animals, MESH: Animals, Medical physics, Instrumentation, Physics, Photosensitizing Agents, Radiation, business.industry, Glioma, Synchrotron, Rats, Radiation therapy, 030220 oncology & carcinogenesis, MESH: Synchrotrons, Monochromatic color, Tomography, X-Ray Computed, MESH: Tomography, X-Ray Computed, business, Synchrotrons
Abstract: International audience; Synchrotrons are opening new paths in innovative anti-cancer radiotherapy strategies. Indeed, the fluence of X-rays induced by synchrotrons is so high (10(6) times higher than standard medical irradiators) that it enables the production of X-ray beams tunable in energy (monochromatic beams) and in size (micrometric beams). Monochromatic synchrotron X-ray beams theoretically permit photoactivate high-Z elements to be introduced in or close to tumours in order to increase the yield of damage by enhanced energy photoabsorption. This is notably the case of attempts with iodinated contrast agents used in tumour imaging (the computed tomography therapy approach) and with platinated agents used in chemotherapy (the PAT-Plat approach). Micrometric synchrotron X-ray beams theoretically permit very high radiation doses to accumulate in tumours by using arrays of parallel microplanar beams that spare the surrounding tissues (the microbeam radiation therapy approach). These anti-cancer applications of synchrotron radiation have been developed at the European Synchrotron Radiation Facility to be applied to glioma, one of the tumour tissues most refractory to standard treatments. In the present paper the molecular and cellular mechanisms involved in these three approaches are reviewed, in the context of recent advances in radiobiology. Furthermore, by considering the unavoidable biases, an attempt to propose a comparison of the different results obtained in preclinical trials dealing with rats bearing tumours is given.
Published: 2007
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19. Calcified aneurysm of the abdominal aorta 12 years after umbilical artery catheterization

Author: Jean-Nicolas Dacher, Denis Laloum, Jacques Watelet, Pascale Thomas, Eléonore Blondiaux, Julie Miquel, Service d'imagerie médicale [CHU Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Service de chirurgie cadiovasculaire et thoracique [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN), Service de Néonatalogie, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Equipe Quantification en Imagerie Fonctionnelle (QuantIF-LITIS), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Université Le Havre Normandie (ULH), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA), Hôpital Charles Nicolle [Rouen], Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, and Breton, Céline
Subjects: Male, Abdominal pain, medicine.medical_specialty, Contrast Media, MESH: Calcinosis, MESH: Ultrasonography, Doppler, Color, 030204 cardiovascular system & hematology, Umbilical Arteries, 030218 nuclear medicine & medical imaging, Blood Vessel Prosthesis Implantation, 03 medical and health sciences, 0302 clinical medicine, Aneurysm, MESH: Catheterization, Peripheral, MESH: Child, MESH: Contrast Media, medicine.artery, Catheterization, Peripheral, medicine, Humans, Radiology, Nuclear Medicine and imaging, Ultrasonography, Doppler, Color, Quadruplet Pregnancy, Child, Neuroradiology, Aorta, MESH: Humans, Respiratory distress, business.industry, Abdominal aorta, Calcinosis, Umbilical artery, MESH: Blood Vessel Prosthesis Implantation, medicine.disease, MESH: Male, 3. Good health, Surgery, MESH: Umbilical Arteries, Pediatrics, Perinatology and Child Health, MESH: Tomography, Spiral Computed, medicine.symptom, business, MESH: Aortic Aneurysm, Abdominal, Tomography, Spiral Computed, Aortic Aneurysm, Abdominal
Abstract: International audience; We report a 12-year-old boy who presented with abdominal pain and who was found to have an aneurysm of the abdominal aorta (AAA). The patient was born from a quadruplet pregnancy induced by in vitro fertilization. Postnatal transient respiratory distress required assisted ventilation that had been monitored by two consecutive umbilical arterial catheters (UAC). AAA is a rare condition in childhood. Infection and/or trauma are known to be the most frequent causes. Most of the reported cases have occurred in children in whom a UAC had been placed during the neonatal period. In this patient the delay between UAC placement and diagnosis was considerable. At the time of this report the patient had remained well during a follow-up of 8 years after treatment.
Published: 2007
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20. Iodixanol in multidetector-row computed tomography angiography (MDCTA): diagnostic accuracy for abdominal aorta and abdominal aortic major-branch diseases using four-, eight- and 16-detector-row CT scanners

Author: Vinay Duddalwar, M. F. Reiser, P. Simons, F. Thouveny, M. Nonent, G. Liessi, O. Cappeliez, L. Bonomo, Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Service d'Imagerie (CHRU - CB), and Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)
Subjects: Male, MESH: Aorta, Abdominal, Contrast Media, 030204 cardiovascular system & hematology, 030218 nuclear medicine & medical imaging, Aortic aneurysm, MESH: Aged, 80 and over, 0302 clinical medicine, Image Processing, Computer-Assisted, Aorta, Abdominal, Computed tomography angiography, Aged, 80 and over, MESH: Aged, MESH: Middle Aged, MESH: Arterial Occlusive Diseases, Radiological and Ultrasound Technology, medicine.diagnostic_test, Abdominal aorta, General Medicine, Middle Aged, MESH: Image Processing, Computer-Assisted, 3. Good health, Radiographic Image Enhancement, MESH: Reproducibility of Results, MESH: Diagnostic Errors, Female, Radiology, MESH: Tomography, X-Ray Computed, MESH: Aortic Aneurysm, Abdominal, medicine.drug, Adult, MESH: Triiodobenzoic Acids, medicine.medical_specialty, Arterial Occlusive Diseases, MESH: Imaging, Three-Dimensional, 03 medical and health sciences, Imaging, Three-Dimensional, Aneurysm, Triiodobenzoic Acids, MESH: Contrast Media, medicine.artery, medicine, Humans, MESH: Angiography, Digital Subtraction, Radiology, Nuclear Medicine and imaging, Diagnostic Errors, Aged, MESH: Humans, business.industry, Angiography, Digital Subtraction, Reproducibility of Results, MESH: Adult, Digital subtraction angiography, medicine.disease, Iodixanol, MESH: Male, Stenosis, MESH: Radiographic Image Enhancement, Angiography, Tomography, X-Ray Computed, business, MESH: Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Aortic Aneurysm, Abdominal
Abstract: Purpose: To compare iodixanol-enhanced multidetector-row computed tomography angiography (MDCTA) with digital subtraction angiography (DSA), perioperative angiography, or surgical findings in the evaluation of the abdominal aorta and its main branches. Material and Methods: 173 patients with known or suspected aortic aneurysms or stenosis/occlusion of the abdominal aorta or its major branches were enrolled. The iso-osmolar contrast medium iodixanol (320 mg Ι/ml) was used, and data were acquired using four-, eight-, or 16-active-detector-row scanners. Reference diagnoses were provided by surgical findings, interventional findings, or DSA. Diagnostic accuracy was estimated with reference to surgery or interventional arteriography or DSA. Image quality was assessed as excellent, good, sufficient, or insufficient, and correlations were made with attenuation values in the aortic lumen. Tolerability of iodixanol was monitored during the injection for discomfort and other adverse events, and for 72 hours after contrast injection. Results: In 132 of 136 evaluable cases, MDCTA diagnosis matched the reference diagnosis, yielding an agreement rate of 97.1% (95% CI 92.6–99.2%). The quality of most MDCTA scans (147/173) was rated as excellent. Overall mean attenuation was 305.7 HU. MDCTA appeared more accurate than DSA for identification of lesion calcification, thrombus, irregularity, and ulceration. Tolerability of iodixanol was good, and no serious adverse events were reported. Conclusion: MDCTA using iodixanol is a promising, noninvasive alternative for evaluating patients with abdominal aortic disease.
Published: 2007
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21. Automated Quantification of Right Ventricular Fat at Contrast-enhanced Cardiac Multidetector CT in Arrhythmogenic Right Ventricular Cardiomyopathy

Author: Arnaud Denis, Pierre Jaïs, François Laurent, Nicolas Derval, Jatin Relan, Tassadit Aït Ali, Hubert Cochet, Michel Haïssaguerre, Frederic Sacher, Maxime Sermesant, Olivier Corneloup, Mélèze Hocini, A Jadidi, Michel Montaudon, Yuki Komatsu, IHU-LIRYC, Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux], University Heart Centre Freiburg - Bad Krozingen, CHU Bordeaux [Bordeaux], St Jude Medical, Analysis and Simulation of Biomedical Images (ASCLEPIOS), Inria Sophia Antipolis - Méditerranée (CRISAM), and Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)
Subjects: Male, medicine.medical_specialty, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, media_common.quotation_subject, Heart Ventricles, Cardiomyopathy, Contrast Media, Multidetector ct, Right ventricular cardiomyopathy, Text mining, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, MESH: Contrast Media, Multidetector computed tomography, Multidetector Computed Tomography, Medicine, Contrast (vision), Humans, Radiology, Nuclear Medicine and imaging, cardiovascular diseases, Prospective Studies, MESH: Arrhythmogenic Right Ventricular Dysplasia, MESH: Multidetector Computed Tomography, Arrhythmogenic Right Ventricular Dysplasia, media_common, MESH: Humans, MESH: Middle Aged, business.industry, Middle Aged, medicine.disease, MESH: Male, MESH: Prospective Studies, 3. Good health, Adipose Tissue, cardiovascular system, Cardiology, Female, MESH: Heart Ventricles, business, MESH: Female, MESH: Adipose Tissue
Abstract: To evaluate an automated method for the quantification of fat in the right ventricular (RV) free wall on multidetector computed tomography (CT) images and assess its diagnostic value in arrhythmogenic RV cardiomyopathy (ARVC).This study was approved by the institutional review board, and all patients gave informed consent. Thirty-six patients with ARVC (mean age ± standard deviation, 46 years ± 15; seven women) were compared with 36 age- and sex-matched subjects with no structural heart disease (control group), as well as 36 patients with ischemic cardiomyopathy (ischemic group). Patients underwent contrast material-enhanced electrocardiography-gated cardiac multidetector CT. A 2-mm-thick RV free wall layer was automatically segmented and myocardial fat, expressed as percentage of RV free wall, was quantified as pixels with attenuation less than -10 HU. Patient-specific segmentations were registered to a template to study fat distribution. Receiver operating characteristic (ROC) analysis was performed to assess the diagnostic value of fat quantification by using task force criteria as a reference.Fat extent was 16.5% ± 6.1 in ARVC and 4.6% ± 2.7 in non-ARVC (P.0001). No significant difference was observed between control and ischemic groups (P = .23). A fat extent threshold of 8.5% of RV free wall was used to diagnose ARVC with 94% sensitivity (95% confidence interval [CI]: 82%, 98%) and 92% specificity (95% CI: 83%, 96%). This diagnostic performance was higher than the one for RV volume (mean area under the ROC curve, 0.96 ± 0.02 vs 0.88 ± 0.04; P = .009). In patients with ARVC, fat correlated to RV volume (R = 0.63, P.0001), RV function (R = -0.67, P = .001), epsilon waves (R = 0.39, P = .02), inverted T waves in V1-V3 (R = 0.38, P = .02), and presence of PKP2 mutations (R = 0.59, P = .02). Fat distribution differed between patients with ARVC and those without, with posterolateral RV wall being the most ARVC-specific area.Automated quantification of RV myocardial fat on multidetector CT images is feasible and performs better than RV volume in the diagnosis of ARVC. Online supplemental material is available for this article.
Published: 2015
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22. Cerebral Venous Thrombosis and Multidetector CT Angiography: Tips and Tricks

Author: Marc Zins, Jean-Michel Colombani, Annick Hélias, Mathieu H. Rodallec, Marie-Christine Jullès, Alexandre Krainik, Véronique Marteau, Antoine Feydy, Service de radiologie, Fondation Hôpital Saint-Joseph, Service d'Imagerie par Résonance Magnétique (IRM), CHU Grenoble, Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Cochin [AP-HP] - Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Beaujon, Dojat, Michel, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Beaujon [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: medicine.medical_specialty, MESH: Cerebral Angiography, medicine.medical_treatment, Venography, Contrast Media, MESH: Phlebography, MESH: Imaging, Three-Dimensional, Multidetector ct, MESH: Cerebral Veins, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, Imaging, Three-Dimensional, 0302 clinical medicine, MESH: Practice Guidelines as Topic, MESH: Contrast Media, Humans, Medicine, Radiology, Nuclear Medicine and imaging, Practice Patterns, Physicians', MESH: Physician's Practice Patterns, Vein, Venous Thrombosis, MESH: Humans, medicine.diagnostic_test, business.industry, Phlebography, Thrombolysis, medicine.disease, Cerebral Veins, Cerebral Angiography, 3. Good health, Radiographic Image Enhancement, Venous thrombosis, medicine.anatomical_structure, MESH: Radiographic Image Enhancement, Maximum intensity projection, Practice Guidelines as Topic, MESH: Venous Thrombosis, Angiography, Radiology, Tomography, Tomography, X-Ray Computed, MESH: Tomography, X-Ray Computed, business, 030217 neurology & neurosurgery
Abstract: International audience; Because of the great diversity of clinical features, its unforeseeable evolution, and a small proportion of cases that will worsen in the acute phase, cerebral venous thrombosis must be diagnosed as early as possible so that specific treatment can be started, typically transcatheter thrombolysis or systemic anticoagulation. Unenhanced computed tomography (CT) is usually the first imaging study performed on an emergency basis. Unenhanced CT allows detection of ischemic changes related to venous insufficiency and sometimes demonstrates a hyperattenuating thrombosed dural sinus or vein. Helical multidetector CT venography with bolus power injection of contrast material and combined use of two-dimensional and three-dimensional reformations (maximum intensity projection, integral display, and volume rendering) provides exquisite anatomic detail of the deep and superficial intracranial venous system and can demonstrate filling defects. However, common variants of the sinovenous system should not be mistaken for sinus thrombosis. A comprehensive diagnostic approach facilitates imaging of cerebral venous thrombosis with multidetector CT.
Published: 2006
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23. Gene expression and gene therapy imaging

Author: Claire Rome, Franck Couillaud, Chrit T. W. Moonen, Salas, Danielle, Imagerie moléculaire et fonctionnelle: de la physiologie à la thérapie, and Université Bordeaux Segalen - Bordeaux 2-IFR8-Centre National de la Recherche Scientifique (CNRS)
Subjects: Diagnostic Imaging, Genetic Markers, medicine.medical_specialty, Pathology, MESH: Gene Expression, Transgene, Genetic enhancement, Genetic Vectors, Contrast Media, Gene Expression, MESH: Transgenes, [SDV.CAN]Life Sciences [q-bio]/Cancer, Computational biology, MESH: Genetic Markers, Vectors in gene therapy, Marker gene, 03 medical and health sciences, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, MESH: Genetic Vectors, Genes, Reporter, MESH: Contrast Media, Gene expression, medicine, Animals, Humans, MESH: Animals, Radiology, Nuclear Medicine and imaging, Transgenes, Gene, 030304 developmental biology, 0303 health sciences, Reporter gene, MESH: Humans, MESH: Diagnostic Imaging, business.industry, MESH: Genes, Reporter, Genetic Therapy, General Medicine, 3. Good health, 030220 oncology & carcinogenesis, Radiology, MESH: Gene Therapy, Molecular imaging, business
Abstract: International audience; The fast growing field of molecular imaging has achieved major advances in imaging gene expression, an important element of gene therapy. Gene expression imaging is based on specific probes or contrast agents that allow either direct or indirect spatio-temporal evaluation of gene expression. Direct evaluation is possible with, for example, contrast agents that bind directly to a specific target (e.g., receptor). Indirect evaluation may be achieved by using specific substrate probes for a target enzyme. The use of marker genes, also called reporter genes, is an essential element of MI approaches for gene expression in gene therapy. The marker gene may not have a therapeutic role itself, but by coupling the marker gene to a therapeutic gene, expression of the marker gene reports on the expression of the therapeutic gene. Nuclear medicine and optical approaches are highly sensitive (detection of probes in the picomolar range), whereas MRI and ultrasound imaging are less sensitive and require amplification techniques and/or accumulation of contrast agents in enlarged contrast particles. Recently developed MI techniques are particularly relevant for gene therapy. Amongst these are the possibility to track gene therapy vectors such as stem cells, and the techniques that allow spatiotemporal control of gene expression by non-invasive heating (with MRI guided focused ultrasound) and the use of temperature sensitive promoters.
Published: 2006
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24. A New Magnetic Resonance Imaging Method for Mapping the Cerebral Blood Volume Fraction: The Rapid Steady-State T1 Method

Author: Adriana T. Perles-Barbacaru, Hana Lahrech, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Association pour la Recherche sur le Cancer (ARC), and Issartel, Jean-Paul
Subjects: Male, Contrast Media, Blood volume, MESH: Rats, Sprague-Dawley, cerebral blood volume fraction, MESH: Magnetic Resonance Imaging, 030218 nuclear medicine & medical imaging, Rats, Sprague-Dawley, Paramagnetism, 0302 clinical medicine, Nuclear magnetic resonance, Heterocyclic Compounds, Image Processing, Computer-Assisted, magnetic resonance imaging, MESH: Animals, MESH: Models, Theoretical, medicine.diagnostic_test, Brain, MESH: Cerebrovascular Circulation, MESH: Image Processing, Computer-Assisted, Neurology, Cerebrovascular Circulation, intravascular paramagnetic contrast agent, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, Cardiology and Cardiovascular Medicine, Hypercapnia, Steady state (electronics), Materials science, MESH: Rats, MESH: Heterocyclic Compounds, longitudinal relaxation time, MESH: Brain, 03 medical and health sciences, Magnetization, Slice preparation, In vivo, rat brain, MESH: Contrast Media, Organometallic Compounds, medicine, Animals, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], business.industry, hypercapnia, MESH: Organometallic Compounds, Magnetic resonance imaging, Models, Theoretical, MESH: Male, Rats, Neurology (clinical), Nuclear medicine, business, 030217 neurology & neurosurgery
Abstract: This paper describes a new rapid steady-state T1 (RSS T1) method for mapping the cerebral blood volume fraction ( CBVf) by magnetic resonance imaging (MRI). The principle is based on a two-compartment model of the brain (intra- and extravascular), and the effects of paramagnetic contrast agents on the intravascular longitudinal relaxation time T1. Using appropriate parameters, an Inversion-Recovery-Fast-Low-Angle-Shot sequence acts like a low pass T1 filter, suppressing signals from tissues with T1≫ TR ( TR=repetition time). It was shown in vivo that, exceeding a particular contrast agent dose, the signal reaches its maximum (corresponding to the intravascular equilibrium magnetization), and is maintained for a duration related to the dose. Acquisitions during this steady state divided by an additional measure of the overall (intra- and extravascular) magnetization at thermal equilibrium provides the CBVf. Experiments were performed on healthy rats at 2.35 T using P760 (Gd3+—compound from Guerbet Laboratories) and Gd-DOTA. Because of its high longitudinal relaxivity, P760 is more convenient, and was used to show the feasibility of the method. The CBVf in different structures of the rat brain was compared. The average CBVf for the whole brain slice is 3.29%±0.69% ( n=15). The influence of transendothelial water exchange was quantified and transversal relaxation effects were found negligible in microvasculature. Finally, the sensitivity of the method to CBVf increases under hypercapnia was evaluated (1%/mm Hg PaCO2), demonstrating its potential for longitudinal studies and functional MRI. Clinical applications are feasible since equivalent results were obtained with Gd-DOTA.
Published: 2006
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25. Angiogenesis: Noninvasive Quantitative Assessment with Contrast-enhanced Functional US in Murine Model

Author: Olivier Lucidarme, Robert F. Mattrey, Judith A. Varner, Sang Hee Choi, Jean Louis Golmard, Yuko Kono, Jacqueline Corbeil, Saidi, Vanessa, Laboratoire d'Ingénierie des Protéines et Contrôle Métabolique, Centre National de la Recherche Scientifique (CNRS), Department of Radiology, University of California (UC), Department of Radiology and Center for Imaging Science, and Sungkyunkwan University [Suwon] (SKKU)
Subjects: Pathology, MESH: Blood Volume, Time Factors, Angiogenesis, Basic fibroblast growth factor, Contrast Media, Blood volume, Neovascularization, MESH: Fluorocarbons, Mice, Random Allocation, chemistry.chemical_compound, MESH: Microcirculation, Medicine, MESH: Animals, MESH: Random Alloc, Skin, Ultrasonography, Fluorocarbons, Blood Volume, Microbubbles, Microvascular Density, Prostheses and Implants, Platelet Endothelial Cell Adhesion Molecule-1, medicine.anatomical_structure, MESH: Models, Animal, Models, Animal, cardiovascular system, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Fibroblast Growth Factor 2, MESH: Image Enhancement, medicine.symptom, MESH: Neovascularization, Physiologic, Blood vessel, medicine.medical_specialty, MESH: Gels, MESH: Prostheses and Implants, Neovascularization, Physiologic, Microcirculation, MESH: Contrast Media, Animals, Humans, Radiology, Nuclear Medicine and imaging, MESH: Mice, [SDV.IB] Life Sciences [q-bio]/Bioengineering, MESH: Humans, MESH: Fibroblast Growth Factor 2, business.industry, MESH: Antigens, CD31, Image Enhancement, chemistry, Implant, business, Gels, MESH: Microbubbles, Biomedical engineering
Abstract: PURPOSE: To evaluate quantitative functional ultrasonography (US) in a murine gel model by using microbubble destruction kinetics to determine whether parametric indices provided with US could help assess angiogenesis. MATERIALS AND METHODS: Institutional Animal Subjects Committee approved experiments and procedures. In 36 normal mice, two 0.4-mL gel implants were placed subcutaneously on either side of spine. One implant contained 0.5, 1.0, or 1.5 microg human basic fibroblast growth factor (bFGF) per milliliter of gel. Functional US quantitative analysis of angiogenesis with microbubble contrast agent was performed on days 3, 6, 9, and 12; histologic data were collected. Time-intensity curve of implant was fitted to mathematic decay model to calculate fractional blood volume and fraction of blood replaced per unit of time. Microvascular density (MVD) and percentage of microvascular area (MVA) were measured after anti-CD31 staining. Spearman rank order correlation was used in analyses. RESULTS: bFGF-containing implants induced MVD of eight, 35, 42, and 42 vessels per square millimeter on days 3, 6, 9, and 12, respectively; in controls, MVD was four vessels/mm2 (P
Published: 2006
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26. Fusion of the midplane with the left intersectional plane: a liver anatomical variation revisited with multidetector-row CT

Author: Jacques Taboury, Laurent Hannoun, Eric Savier, Mehdi Cadi, Philippe Grenier, Olivier Lucidarme, CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Saidi, Vanessa
Subjects: Male, Portal vein, Contrast Media, Computed tomography, 030218 nuclear medicine & medical imaging, Plane (Unicode), MESH: Aged, 80 and over, 0302 clinical medicine, Image Processing, Computer-Assisted, Medicine, Neuroradiology, Aged, 80 and over, MESH: Aged, MESH: Iohexol, MESH: Middle Aged, medicine.diagnostic_test, Portal Vein, Gallbladder, General Medicine, Anatomy, Middle Aged, MESH: Image Processing, Computer-Assisted, medicine.anatomical_structure, Liver, 030220 oncology & carcinogenesis, cardiovascular system, Female, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Radiology, Abnormality, MESH: Tomography, X-Ray Computed, MESH: Gallbladder, Adult, medicine.medical_specialty, Iohexol, MESH: Imaging, Three-Dimensional, Hepatic Veins, 03 medical and health sciences, Imaging, Three-Dimensional, MESH: Contrast Media, Humans, Radiology, Nuclear Medicine and imaging, Vein, Aged, [SDV.IB] Life Sciences [q-bio]/Bioengineering, MESH: Humans, business.industry, MESH: Adult, MESH: Portal Vein, MESH: Male, Right posterior, MESH: Hepatic Veins, Tomography, X-Ray Computed, business, MESH: Female, MESH: Liver
Abstract: This article updates the description of an anatomical variation of the liver, in which the gallbladder is adjacent to the ligamentum teres, that was described until now as "right-sided ligamentum teres and right umbilical portion of the portal vein". A study of eight patients showing this anatomical variation has led to a new archetypal anatomical description of the hepatic and portal veins, using multidetector-row computed tomography (MDCT) with three-dimensional (3D) volume-rendering (VR) reconstructions. While 2D axial imaging gave the same information, MDCT imaging with VR reconstructions provided a clear 3D visualization of this anatomical variation. Typical features can be described as follows: (1) juxtaposition of the ligamentum teres and the gallbladder; (2) typical portal vein branching with a right posterior branch, a left posterior branch and a main medial branch that terminates in the ligamentum teres; (3) two main hepatic veins and a hypotrophied medial hepatic vein. We think, based on the direct comparison of anatomical findings and knowledge of chronological embryological development, that this abnormality results from the defective development of the central part of the liver and not from the persistence of the right rather than the left umbilical vein. Because of the presence of only one medial plane, containing both the gallbladder and the ligamentum teres, we propose renaming it "fusion of hepatic planes".
Published: 2006
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27. Management of postdissection thoracoabdominal aneurysm after previous frozen elephant trunk procedure with the E-vita Open Plus stent-graft

Author: Jean-Philippe Verhoye, Vito G. Ruggieri, Issam Abouliatim, Amedeo Anselmi, Service de chirurgie thoracique cardiaque et vasculaire [Rennes] = Thoracic and Cardiovascular Surgery [Rennes], CHU Pontchaillou [Rennes], Service de chirurgie thoracique cardiaque et vasculaire [Rennes], Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
Subjects: Adult, Male, Reoperation, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Elephant trunks, medicine.medical_treatment, Contrast Media, 030204 cardiovascular system & hematology, MESH: Reoperation, MESH: Blood Vessel Prosthesis, 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, MESH: Contrast Media, medicine, Humans, Thoracoabdominal aneurysm, ComputingMilieux_MISCELLANEOUS, MESH: Aneurysm, Dissecting, MESH: Humans, Hysterectomy, Aortic Aneurysm, Thoracic, medicine.diagnostic_test, business.industry, Stent, MESH: Adult, Magnetic resonance imaging, MESH: Male, Blood Vessel Prosthesis, 3. Good health, Surgery, Computed tomographic angiography, MESH: Stents, Aortic Dissection, 030228 respiratory system, Right heart, MESH: Aortic Aneurysm, Thoracic, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Stents, MESH: Tomography, X-Ray Computed, Tomography, X-Ray Computed, business, Cardiology and Cardiovascular Medicine, Tamoxifen, medicine.drug
Abstract: Extension to the right heart chambers can cause valve obstruction, leading to cardiac insufficiency and death. Magnetic resonance and computed tomographic angiography are the most sensitive methods to diagnose this pathology and to enable planning of the most appropriate therapeutic strategy. Treatment consists in a total hysterectomy and bilateral salpingo-oophorectomy, along with removal of as much of the extrauterine tumor as possible. Recurrence can result from incomplete excision and may necessitate further surgical treatment. Because of the presence of estrogen receptors, antiestrogens such as tamoxifen may be given when the tumor is unresectable or to control for incomplete resection or recurrence of the tumor.
Published: 2012
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28. Absolute perfusion measurements and associated iodinated contrast agent time course in brain metastasis: a study for contrast-enhanced radiotherapy

Author: Obeid, Layal, Deman, Pierre, Tessier, Alexandre, Balosso, Jacques, Estève, François, Adam, Jean-François, Serduc, Raphael, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-CHU Grenoble, Grenoble University Hospital (clinical investigations department and radiation therapy department), and French Ministry of Research
Subjects: MESH: Blood Volume, MESH: Humans, brain perfusion, MESH: Time Factors, MESH: Cerebrovascular Circulation, MESH: Iopamidol, MESH: Injections, Intravenous, MESH: Perfusion Imaging, MESH: Prospective Studies, computed tomography stereotactic radiation therapy, MESH: Contrast Media, MESH: Brain Neoplasms, MESH: Synchrotrons, brain metastasis, contrast agents, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Tissue Distribution, MESH: Radiotherapy Dosage, MESH: Tomography, X-Ray Computed, MESH: Infusions, Intravenous
Abstract: International audience; Contrast-enhanced radiotherapy is an innovative treatment that combines the selective accumulation of heavy elements in tumors with stereotactic irradiations using medium energy X-rays. The radiation dose enhancement depends on the absolute amount of iodine reached in the tumor and its time course. Quantitative, postinfusion iodine biodistribution and associated brain perfusion parameters were studied in human brain metastasis as key parameters for treatment feasibility and quality. Twelve patients received an intravenous bolus of iodinated contrast agent (CA) (40 mL, 4 mL/s), followed by a steady-state infusion (160 mL, 0.5 mL/s) to ensure stable intratumoral amounts of iodine during the treatment. Absolute iodine concentrations and quantitative perfusion maps were derived from 40 multislice dynamic computed tomography (CT) images of the brain. The postinfusion mean intratumoral iodine concentration (over 30 minutes) reached 1.94 ± 0.12 mg/mL. Reasonable correlations were obtained between these concentrations and the permeability surface area product and the cerebral blood volume. To our knowledge, this is the first quantitative study of CA biodistribution versus time in brain metastasis. The study shows that suitable and stable amounts of iodine can be reached for contrast-enhanced radiotherapy. Moreover, the associated perfusion measurements provide useful information for the patient recruitment and management processes.
Published: 2014
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29. Pelvic magnetic resonance imaging in the ewe: a model for experimental gynecologic research

Author: Tristan, Gauthier, Pierre, Marquet, Dorra, Kanoun, Antoine, Maubon, Pascal, Piver, Claude, Couquet, Yves, Aubard, Institut Gustave Roussy (IGR), Université Paris-Sud - Paris 11 (UP11), Pharmacologie des Immunosuppresseurs et de la Transplantation (PIST), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Pharmacologie, toxicologie et pharmacovigilance [CHU Limoges], CHU Limoges, Service de pharmacologie, CHU Toulouse [Toulouse], Service de Radiologie et Imagerie Médicale [CHU Limoges], Université de Limoges (UNILIM), Marquet, Pierre, Service Pharmacologie Clinique [CHU Toulouse], Pôle Santé publique et médecine publique [CHU Toulouse], and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)
Subjects: MESH: Ovary, Contrast Media, Gadolinium, MESH: Magnetic Resonance Imaging, MESH: Contrast Media, Animals, MESH: Animals, Sheep, Domestic, Ovary, Uterus, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Magnetic Resonance Imaging, MESH: Injections, Intravenous, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, MESH: France, MESH: Animals, Inbred Strains, Injections, Intravenous, Feasibility Studies, MESH: Uterus, Female, France, MESH: Sheep, Domestic, MESH: Feasibility Studies, MESH: Gadolinium, MESH: Female, Animals, Inbred Strains
Abstract: International audience; AIM: Our goal was to demonstrate the feasibility of pelvic magnetic resonance imaging (MRI) in the ewe. MATERIAL AND METHODS: Two ewes underwent a pelvic 3 Tesla MRI scan, under light anesthesia, with T2-weighted fast spin-echo images and T1-weighted spoiled gradient-echo images. Multiplan T1 weighted images were also obtained after the intravenous injection of a contrast product. One ewe was anestrous and the other one had undergone ovarian stimulation. RESULTS: No incident occurred during the examination. Both the uterus (with two horns) and the ovaries were identified. The intensity of the endometrial, myometrial and ovarian signals was similar to that encountered in women. The uterus and ovaries could be enhanced in both cases. Pelvic vasculature was also studied. In the case of hormonal stimulation, the endometrium was thicker, follicular growth was identified and enhancement seemed greater. CONCLUSION: MRI in the ewe is feasible and could be helpful in experimental gynecologic research, especially in uterus transplantation.
Published: 2014
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30. Mapping 3-Dimensional Neovessel Organization Steps Using Micro-Computed Tomography in a Murine Model of Hindlimb Ischemia–Brief Report

Author: Oses, Pierre, Renault, Marie-Ange, Chauvel, Rémi, Leroux, Lionel, Chevallereau, Pierre, Allières, Cécile, Daret, Danièle, Lamazière, Jean-Marie Daniel, Dufourcq, Pascale, Couffinhal, Thierry, Duplàa, Cécile, Adaptation cardiovasculaire à l'ischemie, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Supported by The European Vascular Genomics Network (grant #503254), the Fondation de France (grant #2006005678), Fondation pour la Recherche Médicale (DCV20070409258) and Communauté de Travail Pyrénnéenne (CTP). L.L. is the recipient of a grant from the Fondation pour la Recherche Médicale., European Project: 26841,EVGN, Boullé, Christelle, and European Vascular Genomics Network - EVGN - 26841 - OLD
Subjects: Neoprene, Pathology, medicine.medical_specialty, genetic structures, Angiogenesis, Ischemia, Contrast Media, Neovascularization, Physiologic, MESH: Imaging, Three-Dimensional, Hindlimb, Mesenchymal Stem Cell Transplantation, Article, Neovascularization, Mice, MESH: Neoprene, Imaging, Three-Dimensional, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, MESH: Contrast Media, medicine, Animals, MESH: Animals, MESH: Mesenchymal Stem Cell Transplantation, MESH: Mice, Peripheral Vascular Diseases, business.industry, MESH: Hindlimb, MESH: Barium, Mesenchymal stem cell, MESH: Peripheral Vascular Diseases, Hindlimb ischemia, Blood flow, medicine.disease, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Disease Models, Animal, Barium, Arteriogenesis, MESH: Disease Models, Animal, MESH: Ischemia, medicine.symptom, Tomography, X-Ray Computed, MESH: Tomography, X-Ray Computed, Cardiology and Cardiovascular Medicine, business, MESH: Neovascularization, Physiologic
Abstract: Objectives— Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization. Methods and Results— We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches. Conclusion— This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation.
Published: 2009
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31. Absolute perfusion measurements and associated iodinated contrast agent time course in brain metastasis: a study for contrast-enhanced radiotherapy.: Perfusion in [I]-enhanced radiotherapy

Author: Alexandre Tessier, Jean-François Adam, François Estève, Jacques Balosso, Layal Obeid, Pierre Deman, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Grenoble, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-CHU Grenoble, Grenoble University Hospital (clinical investigations department and radiation therapy department), and French Ministry of Research
Subjects: MESH: Blood Volume, Time Factors, medicine.medical_treatment, Contrast Media, Perfusion scanning, Iopamidol, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, brain metastasis, Tissue Distribution, Prospective Studies, MESH: Radiotherapy Dosage, Infusions, Intravenous, Blood Volume, brain perfusion, Brain Neoplasms, Radiotherapy Dosage, MESH: Cerebrovascular Circulation, 3. Good health, Neurology, 030220 oncology & carcinogenesis, Cerebrovascular Circulation, MESH: Brain Neoplasms, Injections, Intravenous, MESH: Synchrotrons, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Original Article, Radiology, MESH: Tomography, X-Ray Computed, Cardiology and Cardiovascular Medicine, Perfusion, medicine.drug, medicine.medical_specialty, Perfusion Imaging, chemistry.chemical_element, Iodinated Contrast Agent, Stereotactic radiation therapy, MESH: Iopamidol, Iodine, 03 medical and health sciences, MESH: Contrast Media, medicine, Humans, contrast agents, Multislice, MESH: Tissue Distribution, MESH: Infusions, Intravenous, MESH: Humans, business.industry, MESH: Time Factors, medicine.disease, MESH: Injections, Intravenous, MESH: Perfusion Imaging, MESH: Prospective Studies, computed tomography stereotactic radiation therapy, chemistry, Neurology (clinical), Nuclear medicine, business, Tomography, X-Ray Computed, Synchrotrons, Brain metastasis
Abstract: International audience; Contrast-enhanced radiotherapy is an innovative treatment that combines the selective accumulation of heavy elements in tumors with stereotactic irradiations using medium energy X-rays. The radiation dose enhancement depends on the absolute amount of iodine reached in the tumor and its time course. Quantitative, postinfusion iodine biodistribution and associated brain perfusion parameters were studied in human brain metastasis as key parameters for treatment feasibility and quality. Twelve patients received an intravenous bolus of iodinated contrast agent (CA) (40 mL, 4 mL/s), followed by a steady-state infusion (160 mL, 0.5 mL/s) to ensure stable intratumoral amounts of iodine during the treatment. Absolute iodine concentrations and quantitative perfusion maps were derived from 40 multislice dynamic computed tomography (CT) images of the brain. The postinfusion mean intratumoral iodine concentration (over 30 minutes) reached 1.94 ± 0.12 mg/mL. Reasonable correlations were obtained between these concentrations and the permeability surface area product and the cerebral blood volume. To our knowledge, this is the first quantitative study of CA biodistribution versus time in brain metastasis. The study shows that suitable and stable amounts of iodine can be reached for contrast-enhanced radiotherapy. Moreover, the associated perfusion measurements provide useful information for the patient recruitment and management processes.
Published: 2013
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32. In vivo imaging of vessel diameter, size, and density: a comparative study between MRI and histology

Author: Samuel Valable, Emmanuel L. Barbier, Alexandre Krainik, Chantal Rémy, Benjamin Lemasson, Régine Farion, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale, Centre-Imagerie, Neurosciences, et Application aux Pathologies (CI-NAPS - UMR 6232), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service de neuroradiologie [Grenoble], CHU Grenoble-CHU Grenoble, Ligue contre le cancer, ARC, Institut National du Cancer, Région Rhône-Alpes, Cancéropôle Lyon Auvergne Rhône-Alpes, Neuro-imagerie fonctionnelle et métabolique ( ANTE-INSERM U836, équipe 5 ), Grenoble Institut des Neurosciences ( GIN ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ), Centre-Imagerie, Neurosciences, et Application aux Pathologies ( CI-NAPS - UMR 6232 ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Grenoble Institut des Neurosciences ( GIN ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Service de neuroradiologie [Grenoble], and CHU Grenoble
Subjects: Pathology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH : Corpus Striatum, [ SPI.SIGNAL ] Engineering Sciences [physics]/Signal and Image processing, MESH : Iron, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, 030218 nuclear medicine & medical imaging, MESH: Corpus Striatum, MESH: Magnetic Resonance Imaging, MESH: Glioma, MESH: Neocortex, 0302 clinical medicine, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, glioma, MESH: Animals, MESH : Rats, Inbred F344, MESH: Iron, medicine.diagnostic_test, MESH : Rats, [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Brain Neoplasms, cardiovascular system, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], MESH : Histological Techniques, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Perfusion, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Preclinical imaging, microvasculature, MRI, medicine.medical_specialty, [ INFO.INFO-TS ] Computer Science [cs]/Signal and Image Processing, MESH: Rats, MESH : Male, brain, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Histological Techniques, Biology, MESH : Rats, Wistar, perfusion, 03 medical and health sciences, MESH: Brain, In vivo, MESH : Magnetic Resonance Imaging, Glioma, MESH: Contrast Media, [ PHYS.PHYS.PHYS-MED-PH ] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], medicine, MESH : Neoplasm Transplantation, Radiology, Nuclear Medicine and imaging, MESH : Brain Neoplasms, MESH : Neocortex, MESH : Contrast Media, business.industry, MESH: Rats, Inbred F344, Mean Vessel Diameter, Histology, Magnetic resonance imaging, MESH: Rats, Wistar, medicine.disease, USPIO, MESH: Male, MESH : Brain, MESH : Glioma, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Animals, Nuclear medicine, business, 030217 neurology & neurosurgery, Ex vivo, MESH: Neoplasm Transplantation
Abstract: International audience; The aim of this study was to compare magnetic resonance imaging (MRI) and histological estimates of the mean vessel diameter (mVD), the vessel density (Density), and the vessel size index (VSI) obtained in the same tumor-bearing animals. Twenty-seven rats bearing intracranial glioma (C6 or RG2) were imaged by MRI. Changes in transverse relaxations (ΔR 2* and R(2)) were induced by the injection of an iron-based contrast agent and were mapped using a multi gradient-echo spin-echo sequence. Then, brain vascular network was studied ex vivo by histology. Three regions of interest were drawn in apparently normal tissue (neocortex and striatum) and in the tumor. In vivo mVD(MRI), Density(MRI), and VSI(MRI) were measured; ex vivo, mVD(histo), Density(histo), and VSI(histo) were quantified on the same animals. MRI and histology measurements differed by -15 to 26%. A positive correlation was found between MRI and histology for mVD, Density, and VSI counterparts (R(2) = 0.62, 0.50, 0.73, respectively; P < 0.001 in all cases). This study indicates that MRI and histology yields well correlated the estimates of mVD, Density, and VSI. VSI is the closest MRI estimate to histology. As Density and mVD or VSI provide complementary information, it is worth computing them to characterize angiogenesis beyond blood volume fraction.
Published: 2013
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33. A simulation tool for dynamic contrast enhanced MRI

Author: Emmanuel L. Barbier, Clément Debacker, Thomas Christen, Nicolas Pannetier, Franck Mauconduit, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Radiology [Stanford], Stanford Medicine, Stanford University-Stanford University, This work was funded by the Institut National du Cancer, the Association pour la Recherche sur le Cancer and the Ministry for Higher Education and Research of France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., and Dojat, Michel
Subjects: Anatomy and Physiology, Medical Physics, Tumor Physiology, lcsh:Medicine, Contrast Media, Vascular permeability, Blood volume, computer.software_genre, Cardiovascular, 030218 nuclear medicine & medical imaging, Diagnostic Radiology, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Voxel, Basic Cancer Research, Diffusion (business), Cardiovascular Imaging, lcsh:Science, Physics, Multidisciplinary, Magnetic Resonance Imaging, MESH: Reproducibility of Results, Oncology, MESH: Permeability, Dynamic contrast-enhanced MRI, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], Medicine, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Image Enhancement, Radiology, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Algorithms, Research Article, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, MESH: Algorithms, Thermal diffusivity, Permeability, 03 medical and health sciences, MESH: Computer Simulation, MESH: Contrast Media, Image Interpretation, Computer-Assisted, Humans, Computer Simulation, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Computerized Simulations, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, [PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], MESH: Humans, business.industry, lcsh:R, Reproducibility of Results, Blood flow, MESH: Blood Vessels, Image Enhancement, Permeability (electromagnetism), Regional Blood Flow, Computer Science, Blood Vessels, lcsh:Q, MESH: Regional Blood Flow, Nuclear medicine, business, Physiological Processes, computer, MESH: Image Interpretation, Computer-Assisted, 030217 neurology & neurosurgery, Biomedical engineering
Abstract: International audience; The quantification of bolus-tracking MRI techniques remains challenging. The acquisition usually relies on one contrast and the analysis on a simplified model of the various phenomena that arise within a voxel, leading to inaccurate perfusion estimates. To evaluate how simplifications in the interstitial model impact perfusion estimates, we propose a numerical tool to simulate the MR signal provided by a dynamic contrast enhanced (DCE) MRI experiment. Our model encompasses the intrinsic R1 and R2 relaxations, the magnetic field perturbations induced by susceptibility interfaces (vessels and cells), the diffusion of the water protons, the blood flow, the permeability of the vessel wall to the the contrast agent (CA) and the constrained diffusion of the CA within the voxel. The blood compartment is modeled as a uniform compartment. The different blocks of the simulation are validated and compared to classical models. The impact of the CA diffusivity on the permeability and blood volume estimates is evaluated. Simulations demonstrate that the CA diffusivity slightly impacts the permeability estimates (< 5% for classical blood flow and CA diffusion). The effect of long echo times is investigated. Simulations show that DCE-MRI performed with an echo time TE = 5 ms may already lead to significant underestimation of the blood volume (up to 30% lower for brain tumor permeability values). The potential and the versatility of the proposed implementation are evaluated by running the simulation with realistic vascular geometry obtained from two photons microscopy and with impermeable cells in the extravascular environment. In conclusion, the proposed simulation tool describes DCE-MRI experiments and may be used to evaluate and optimize acquisition and processing strategies.
Published: 2013
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34. In vivo imaging of vessel diameter, size, and density: a comparative study between MRI and histology.: Vessel diameter, size and density: MRI vs histology

Author: Lemasson, Benjamin, Valable, Samuel, Farion, Régine, Krainik, Alexandre, Rémy, Chantal, Barbier, Emmanuel, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale, Centre-Imagerie, Neurosciences, et Application aux Pathologies (CI-NAPS - UMR 6232), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service de neuroradiologie [Grenoble], CHU Grenoble-CHU Grenoble, Ligue contre le cancer, ARC, Institut National du Cancer, Région Rhône-Alpes, Cancéropôle Lyon Auvergne Rhône-Alpes, and Dojat, Michel
Subjects: MESH: Rats, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, brain, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, [SDV.CAN]Life Sciences [q-bio]/Cancer, MESH: Histological Techniques, perfusion, MESH: Corpus Striatum, MESH: Magnetic Resonance Imaging, MESH: Glioma, MESH: Brain, MESH: Neocortex, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, [SDV.CAN] Life Sciences [q-bio]/Cancer, glioma, MESH: Contrast Media, MESH: Animals, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, MESH: Iron, [PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], MESH: Rats, Inbred F344, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, MESH: Rats, Wistar, USPIO, MESH: Male, MESH: Brain Neoplasms, cardiovascular system, [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, MESH: Neoplasm Transplantation, microvasculature, MRI
Abstract: International audience; The aim of this study was to compare magnetic resonance imaging (MRI) and histological estimates of the mean vessel diameter (mVD), the vessel density (Density), and the vessel size index (VSI) obtained in the same tumor-bearing animals. Twenty-seven rats bearing intracranial glioma (C6 or RG2) were imaged by MRI. Changes in transverse relaxations (ΔR 2* and R(2)) were induced by the injection of an iron-based contrast agent and were mapped using a multi gradient-echo spin-echo sequence. Then, brain vascular network was studied ex vivo by histology. Three regions of interest were drawn in apparently normal tissue (neocortex and striatum) and in the tumor. In vivo mVD(MRI), Density(MRI), and VSI(MRI) were measured; ex vivo, mVD(histo), Density(histo), and VSI(histo) were quantified on the same animals. MRI and histology measurements differed by -15 to 26%. A positive correlation was found between MRI and histology for mVD, Density, and VSI counterparts (R(2) = 0.62, 0.50, 0.73, respectively; P < 0.001 in all cases). This study indicates that MRI and histology yields well correlated the estimates of mVD, Density, and VSI. VSI is the closest MRI estimate to histology. As Density and mVD or VSI provide complementary information, it is worth computing them to characterize angiogenesis beyond blood volume fraction.
Published: 2013
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35. Follow-up of coiled intracranial aneurysms: comparison of 3D time-of-flight and contrast-enhanced magnetic resonance angiography at 3T in a large, prospective series

Author: Christophe Portefaix, A. Boulin, Jean-Yves Gauvrit, Laurent Pierot, Hôpital Maison Blanche, Centre Hospitalier Universitaire de Reims (CHU Reims), Centre de Recherche en Sciences et Technologies de l'Information et de la Communication - EA 3804 (CRESTIC), Université de Reims Champagne-Ardenne (URCA), Service de neuroradiologie [Suresnes], Hôpital Foch [Suresnes], Service de radiologie et imagerie médicale [Rennes] = Radiology [Rennes], CHU Pontchaillou [Rennes], Vision, Action et Gestion d'informations en Santé (VisAGeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE (IRISA-D5), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), and Gauvrit, Jean-Yves
Subjects: Male, Contrast Media, MESH: Magnetic Resonance Angiography, Magnetic resonance angiography, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, Occlusion, Prospective Studies, Neuroradiology, MESH: Aged, MESH: Middle Aged, medicine.diagnostic_test, Interventional radiology, Prostheses and Implants, General Medicine, MESH: Follow-Up Studies, Middle Aged, cardiovascular system, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Radiology, Adult, medicine.medical_specialty, MESH: Prostheses and Implants, MESH: Intracranial Aneurysm, 03 medical and health sciences, Aneurysm, MESH: Contrast Media, medicine, Humans, Radiology, Nuclear Medicine and imaging, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], cardiovascular diseases, Aged, MESH: Humans, business.industry, Intracranial Aneurysm, Magnetic resonance imaging, MESH: Adult, Gold standard (test), medicine.disease, eye diseases, MESH: Prospective Studies, MESH: Male, Angiography, business, MESH: Female, Magnetic Resonance Angiography, 030217 neurology & neurosurgery, Follow-Up Studies
Abstract: To compare 3D-TOF magnetic resonance angiography (MRA) and contrast-enhanced MRA (CE-MRA) sequences at 3T in the follow-up of coiled aneurysms with digital subtracted angiography (DSA) as the gold standard.DSA, 3D-TOF and CE-MRA were performed in a prospective series of 126 aneurysms in 96 patients (57 female, 39 male; age: 25-75 years, mean: 51.3 ± 11.3 years). The quality of aneurysm occlusion was assessed independently and anonymously by a core laboratory.Using DSA (gold standard technique), total occlusion was depicted in 57 aneurysms (45.2%), neck remnant in 34 aneurysms (27.0%) and aneurysm remnant in 35 aneurysms (27.8%). Sensitivity, specificity, positive predictive value and negative predictive value were very similar with 3D-TOF and CE-MRA. Visibility of coils was much better with 3D-TOF (95.2%) than with CE-MRA (23.0%) (P 0.001). Also, substantial artefacts were less frequent with 3D-TOF (4.0%) than with CE-MRA (11.9%; P = 0.012).In this large prospective series of patients with coiled aneurysms, at 3T 3D-TOF MRA was equivalent to CE-MRA for the evaluation of aneurysm occlusion, but coil visibility was superior at 3D-TOF. Thus the use of 3D-TOF at 3T is recommended for the follow-up of coiled intracranial aneurysms.• Different Magnetic Resonance (MR) imaging techniques are used to evaluate intracranial aneurysms. • At 3T MR, 3D-TOF and CE-MRA appear equivalent for evaluating coiled aneurysms.. • Coils are better visualised on 3D-TOF than on CE-MRA. • Combined analysis of 3D-TOF and CE-MRA does not seem helpful. • At 3T, 3D-TOF techniques are recommended for monitoring patients with coiled aneurysms.
Published: 2012
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36. A tripodal ruthenium-gadolinium metallostar as a potential α(v)β(3) integrin specific bimodal imaging contrast agent

Author: Sophie Laurent, Carmen Burtea, Tatjana N. Parac-Vogt, Svetlana V. Eliseeva, Luce Vander Elst, Peter Verwilst, Stéphane Petoud, Wim M. De Borggraeve, Robert N. Muller, Institut des Sciences Moléculaires (ISM), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Université Sciences et Technologies - Bordeaux 1-Université Montesquieu - Bordeaux 4-Institut de Chimie du CNRS (INC), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), NMR and Molecular Imaging Laboratory [Mons], University of Mons [Belgium] (UMONS), General, Organic and Biomedical Chemistry, UMONS, and Center for Microscopy and Molecular Imaging (CMMI), Académie Wallonie-Bruxelles
Subjects: Models, Molecular, Gadolinium, MRI contrast agent, media_common.quotation_subject, chemistry.chemical_element, Quantum yield, Contrast Media, 010402 general chemistry, 01 natural sciences, Ruthenium, MESH: Magnetic Resonance Imaging, Inorganic Chemistry, Turn (biochemistry), Jurkat Cells, Nuclear magnetic resonance, MESH: Contrast Media, MESH: Jurkat Cells, Contrast (vision), Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Luminescent Agents, Physical and Theoretical Chemistry, media_common, Luminescent Agents, MESH: Humans, 010405 organic chemistry, Chemistry, Integrin alphaVbeta3, MESH: Ruthenium, Magnetic Resonance Imaging, 0104 chemical sciences, MESH: Integrin alphaVbeta3, MESH: Oligopeptides, Absorption (chemistry), Luminescence, MESH: Gadolinium, Oligopeptides, MESH: Models, Molecular
Abstract: International audience; Gd(III)-containing metallostar contrast agents are gaining increased attention, because their architecture allows for a slower tumbling rate, which, in turn, results in larger relaxivities. So far, these metallostars find possible applications as blood pool contrast agents. In this work, the first example of a tissue-selective metallostar contrast agent is described. This RGD-peptide decorated Ru(II)(Gd(III))(3)metallostar is synthesized as an α(v)β(3)-integrin specific contrast agent, with possible applications in the detection of atherosclerotic plaques and tumor angiogenesis. The contrast agent showed a relaxivity of 9.65 s(-1) mM(-1), which represents an increase of 170%, compared to a low-molecular-weight analogue, because of a decreased tumbling rate (τ(R) = 470 ps). The presence of the MLCT band (absorption 375-500 nm, emission 525-850 nm) of the central Ru(II)(Ph-Phen)(3)-based complex grants the metallostar attractive luminescent properties. The (3)MLCT emission is characterized by a quantum yield of 4.69% and a lifetime of 804 ns, which makes it an interesting candidate for time-gated luminescence imaging. The potential application as a selective MRI contrast agent for α(v)β(3)-integrin expressing tissues is shown by an in vitro relaxometric analysis, as well as an in vitroT(1)-weighted MR image.
Published: 2012
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37. Invasive lobular carcinoma of the breast: MRI pathological correlation following bilateral total mastectomy

Author: Pauline Beaussart, T.-H. Dao, Alain Luciani, Alain Rahmouni, Elie Calitchi, Laurent Lantieri, Laurence Baranes, Yazid Belkacemi, Evelyne Meyblum, Jean-Léon Lagrange, Zahira Merabet, Julie Perlbarg, Frederic Pigneur, Christophe Lepage, Aude Stivalet, Service d'imagerie médicale, Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Inserm U955, équipe 17, Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) - Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) - Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM) - IFR10 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) - Institut National de la Santé et de la Recherche Médicale (INSERM) - IFR10, Département de pathologie [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) - Hôpital Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'informatique médicale et biostatistiques [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Necker - Enfants Malades [AP-HP], Service de radiothérapie [Mondor], Service de chirurgie plastique et reconstructive [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and Guellaen, Georges
Subjects: Pathology, MESH: Combined Modality Therapy, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Mammaplasty, Lobular carcinoma, Contrast Media, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, MESH: Mastectomy, Simple, 0302 clinical medicine, MESH: Carcinoma, Lobular, Risk Factors, MESH: Risk Factors, Medicine, Breast MRI, Total Mastectomy, skin and connective tissue diseases, Pathological correlation, MESH: Aged, MESH: Middle Aged, Radiological and Ultrasound Technology, medicine.diagnostic_test, Biopsy, Needle, General Medicine, Middle Aged, Invasive ductal carcinoma, Combined Modality Therapy, Magnetic Resonance Imaging, MESH: Predictive Value of Tests, 030220 oncology & carcinogenesis, Invasive lobular carcinoma, Female, Radiology, Mammography, Adult, medicine.medical_specialty, MESH: Biopsy, Needle, MESH: Mammography, [SDV.CAN]Life Sciences [q-bio]/Cancer, Breast Neoplasms, Lobular Neoplasm, MESH: Meglumine, Sensitivity and Specificity, 03 medical and health sciences, Meglumine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Predictive Value of Tests, MESH: Contrast Media, Image Interpretation, Computer-Assisted, Organometallic Compounds, Humans, Neoplasm Invasiveness, Radiology, Nuclear Medicine and imaging, Aged, Mastectomy, Simple, Retrospective Studies, MESH: Humans, business.industry, Magnetic resonance imaging, MESH: Adult, MESH: Organometallic Compounds, MESH: Retrospective Studies, MESH: Neoplasm Invasiveness, medicine.disease, MESH: Sensitivity and Specificity, body regions, Carcinoma, Lobular, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Mammaplasty, business, MESH: Female, MESH: Image Interpretation, Computer-Assisted, MESH: Breast Neoplasms
Abstract: Background Invasive lobular carcinoma (ILC) is more often multifocal and bilateral than invasive ductal carcinoma. MRI is usually recommended for detection of all ILC sites. The performance of known diagnostic breast MRI criteria for ILC characterization has not been evaluated to date using bilateral mastectomy specimens as gold standard. Purpose To determine the value of BI-RADS 2006 MRI criteria for ILC detection and characterization, using pathological examination of bilateral mastectomy specimens as the reference standard. Material and Methods Between 2004 and 2007, we retrospectively included all patients with pathologically documented ILC referred to our institution for bilateral mastectomy and preoperative bilateral breast MRI. The location, diameter, and characteristics (BI-RADS) of all lesions were compared with pathological findings. The sensitivity and positive predictive value of bilateral breast MRI for the diagnosis of ILC were calculated. Association of MRI BI-RADS categorical variables and characterization of ILC were assessed (Fisher exact test). Results Among 360 patients treated for ILC in 2004–2007, 15 patients qualified for this study. Thirty-one ILC foci were found on pathological examination (30 ipsilateral and 1 contralateral tumor; mean diameter 23 mm; range 2–60 mm) and all were identified on MRI, with 90% of masses and 10% non-mass-like enhancements; MRI features significantly associated with ILC included absence of smooth margins ( P = 0.02) and rim-shaped enhancement ( P = 0.039). Enhancement kinetics of the 31 foci were evenly distributed among wash-out, plateau, and persistent profiles. Eleven additional lesions were seen on MRI, mainly corresponding to fibrocystic disease; 91% presented as masses and 9% had a wash-out profile. Conclusion Based on the 2006 BI-RADS criteria, breast MRI shows a high sensitivity for ILC detection, at the expense of a 26% false-positive rate, suggesting that a pathological proof by US- or MR-guided biopsy is required in case of suspicious MRI images in this context.
Published: 2012
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38. How stereological analysis of vascular morphology can quantify the blood volume fraction as a marker for tumor vasculature: comparison with magnetic resonance imaging.: Blood volume quantification by stereology and MRI

Author: Adriana T. Perles-Barbacaru, Régine Farion, Hana Lahrech, Boudewijn van der Sanden, Inserm U836, équipe 7, Nanomédecine et cerveau, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), and The study was funded by PhD and post doc stipends from the French Cancer Research Association (ARC, Association pour la Recherche sur le Cancer) and the French National Cancer Institute(INCa, Institut National du Cancer).
Subjects: Models, Anatomic, Pathology, MESH: Blood Volume, Angiogenesis, Gadolinium, Contrast Media, Stereology, MESH: Microscopy, Fluorescence, MESH: Models, Anatomic, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, MESH: Glioma, 0302 clinical medicine, Heterocyclic Compounds, Image Processing, Computer-Assisted, Medicine, MESH: Animals, MESH: Brain Mapping, Brain Mapping, Blood Volume, Blood Volume Determination, Neovascularization, Pathologic, medicine.diagnostic_test, Brain Neoplasms, Glioma, Magnetic Resonance Imaging, MESH: Image Processing, Computer-Assisted, vascular volume fraction, MESH: Microvessels, Neurology, Blood Volume Fraction, MESH: Brain Neoplasms, Original Article, RSST1-MRI, Cardiology and Cardiovascular Medicine, medicine.medical_specialty, MESH: Cell Line, Tumor, MESH: Rats, MESH: Heterocyclic Compounds, MESH: Blood Volume Determination, chemistry.chemical_element, [SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine, Tumor vasculature, 03 medical and health sciences, vessel diameter, Cell Line, Tumor, MESH: Contrast Media, Organometallic Compounds, Animals, Rats, Wistar, business.industry, Magnetic resonance imaging, MESH: Organometallic Compounds, MESH: Rats, Wistar, Rats, Microscopy, Fluorescence, Vascular morphology, chemistry, blood volume fraction, Microvessels, stereology, Neurology (clinical), business, MESH: Neovascularization, Pathologic, Neoplasm Transplantation, 030217 neurology & neurosurgery, MESH: Neoplasm Transplantation, GADOTERATE MEGLUMINE
Abstract: To assess angiogenesis noninvasively in a C6 rat brain tumor model, the rapid-steady-state- T1 (RSST1) magnetic resonance imaging (MRI) method was used for microvascular blood volume fraction (BVf) quantification with a novel contrast agent gadolinium per (3,6 anhydro) α-cyclodextrin (Gd-ACX). In brain tissue contralateral to the tumor, equal BVfs were obtained with Gd-ACX and the clinically approved gadoterate meglumine (Gd-DOTA). Contrary to Gd-DOTA, which leaks out of the tumor vasculature, Gd-ACX was shown to remain vascular in the tumor tissue allowing quantification of the tumor BVf. We sought to confirm the obtained tumor BVf using an independent method: instead of using a ‘standard’ two-dimensional histologic method, we study here how vascular morphometry combined with a stereological technique can be used for three-dimensional assessment of the vascular volume fraction ( VV). The VV is calculated from the vascular diameter and length density. First, the technique is evaluated on simulated data and the healthy rat brain vasculature and is then applied to the same C6 tumor vasculature previously quantified by RSST1-MRI with Gd-ACX. The mean perfused VV and the BVf obtained by MRI in tumor regions are practically equal and the technique confirms the spatial heterogeneity revealed by MRI.
Published: 2012
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39. Vessel size index measurements in a rat model of glioma: comparison of the dynamic (Gd) and steady-state (iron-oxide) susceptibility contrast MRI approaches

Author: Pannetier, Nicolas, Lemasson, Benjamin, Christen, Thomas, Tachrount, Mohamed, Troprès, Irène, Farion, Régine, Segebarth, Christoph, Rémy, Chantal, Barbier, Emmanuel, Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Oncodesign [Dijon], RMN biomédicale : de la cellule à l'homme (RBCH), Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-DIR CENTRALE DU SSA-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-DIR CENTRALE DU SSA-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National du Cancer Cancéropôle Lyon Auvergne Rhône-Alpes Association pour la Recherche sur le Cancer - Région Rhône-Alpes, and Dojat, Michel
Subjects: Male, MESH: Cell Line, Tumor, Gd, MESH: Rats, MESH: Organ Size, [INFO.INFO-IM] Computer Science [cs]/Medical Imaging, Contrast Media, MESH: Magnetite Nanoparticles, Gadolinium, vessel size index, Spiral, DSC, MESH: Magnetic Resonance Imaging, Diffusion, MESH: Glioma, Cell Line, Tumor, glioma, MESH: Contrast Media, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Animals, Humans, MESH: Animals, MESH: Dextrans, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Rats, Wistar, Magnetite Nanoparticles, MESH: Humans, Brain Neoplasms, VSI, Dextrans, MESH: Diffusion, Organ Size, MESH: Blood Vessels, MESH: Rats, Wistar, Magnetic Resonance Imaging, USPIO, MESH: Male, Rats, Disease Models, Animal, MESH: Brain Neoplasms, Blood Vessels, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Disease Models, Animal, MESH: Gadolinium, Neoplasm Transplantation, MESH: Neoplasm Transplantation, MRI
Abstract: International audience; Vessel size index (VSI), a parameter related to the distribution of vessel diameters, may be estimated using two MRI approaches: (i) dynamic susceptibility contrast (DSC) MRI following the injection of a bolus of Gd-chelate. This technique is routinely applied in the clinic to assess intracranial tissue perfusion in patients; (ii) steady-state susceptibility contrast with USPIO contrast agents, which is considered here as the standard method. Such agents are not available for human yet and the steady-state approach is currently limited to animal studies. The aim is to compare VSI estimates obtained with these two approaches on rats bearing C6 glioma (n = 7). In a first session, VSI was estimated from two consecutive injections of Gd-Chelate (Gd(1) and Gd(2)). In a second session (4 hours later), VSI was estimated using USPIO. Our findings indicate that both approaches yield comparable VSI estimates both in contralateral (VSI{USPIO} = 7.5 ± 2.0 µm, VSI{Gd(1)} = 6.5 ± 0.7 µm) and in brain tumour tissues (VSI{USPIO} = 19.4 ± 7.1 µm, VSI{Gd(1)} = 16.6 ± 4.5 µm). We also observed that, in the presence of BBB leakage (as it occurs typically in brain tumours), applying a preload of Gd-chelate improves the VSI estimate with the DSC approach both in contralateral (VSI{Gd(2)} = 7.1 ± 0.4 µm) and in brain tumour tissues (VSI{Gd(2)} = 18.5 ± 4.3 µm) but is not mandatory. VSI estimates do not appear to be sensitive to T(1) changes related to Gd extravasation. These results suggest that robust VSI estimates may be obtained in patients at 3 T or higher magnetic fields with the DSC approach.
Published: 2012
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40. Isoquinoline-based lanthanide complexes: bright NIR optical probes and efficient MRI agents

Author: Célia S. Bonnet, Stéphane Petoud, Fabien Caillé, Lothar Helm, Sandrine Villette, Franck Suzenet, Éva Tóth, Frédéric Buron, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Chimie Organique et Analytique (ICOA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO)-Institut de Chimie du CNRS (INC), Laboratoire de Chimie Inorganique et Bioinorganique (LCIB), and Ecole Polytechnique Fédérale de Lausanne (EPFL)
Subjects: Lanthanide, Luminescence, RELAXIVITY, PRESSURE NMR KINETICS, Analytical chemistry, Contrast Media, RELAXATION, NEAR-INFRARED LUMINESCENT, Inner sphere electron transfer, 010402 general chemistry, MESH: Spectroscopy, Near-Infrared, 01 natural sciences, Lanthanoid Series Elements, MESH: Magnetic Resonance Imaging, Inorganic Chemistry, Transmetalation, MESH: Lanthanoid Series Elements, MESH: Coordination Complexes, Coordination Complexes, MESH: Contrast Media, MESH: Isoquinolines, Molecule, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Chelation, O-17 NMR, MESH: Luminescent Agents, Physical and Theoretical Chemistry, Aqueous solution, Luminescent Agents, Spectroscopy, Near-Infrared, STABILITY, 010405 organic chemistry, Chemistry, MESH: Luminescence, Isoquinolines, Magnetic Resonance Imaging, 0104 chemical sciences, WATER-EXCHANGE, GADOLINIUM COMPLEXES, CATIONS, Physical chemistry, Thermodynamics, Chemical stability, CONTRAST AGENTS, MESH: Thermodynamics
Abstract: International audience; In the objective of developing ligands that simultaneously satisfy the requirements for MRI contrast agents and near-infrared emitting optical probes that are suitable for imaging, three isoquinoline-based polyaminocarboxylate ligands, L1, L2 and L3, have been synthesized and the corresponding Gd(3+), Nd(3+) and Yb(3+) complexes investigated. The specific challenge of the present work was to create NIR emitting agents which (i) have excitation wavelengths compatible with biological applications and (ii) are able to emit a sufficient number of photons to ensure sensitive NIR detection for microscopic imaging. Here we report the first observation of a NIR signal arising from a Ln(3+) complex in aqueous solution in a microscopy setup. The lanthanide complexes have high thermodynamic stability (log K(LnL) =17.7-18.7) and good selectivity for lanthanide ions versus the endogenous cations Zn(2+), Cu(2+), and Ca(2+) thus preventing transmetalation. A variable temperature and pressure (17)O NMR study combined with nuclear magnetic relaxation dispersion measurements yielded the microscopic parameters characterizing water exchange and rotation. Bishydration of the lanthanide cation in the complexes, an important advantage to obtain high relaxivity for the Gd(3+) chelates, has been demonstrated by (17)O chemical shifts for the Gd(3+) complexes and by luminescence lifetime measurements for the Yb(3+) analogues. The water exchange on the three Gd(3+) complexes is considerably faster (k(ex)(298) = (13.9-15.4) × 10(6) s(-1)) than on commercial Gd(3+)-based contrast agents and proceeds via a dissociative mechanism, as evidenced by the large positive activation volumes for GdL1 and GdL2 (+10.3 ± 0.9 and +10.6 ± 0.9 cm(3) mol(-1), respectively). The relaxivity of GdL1 is doubled at 40 MHz and 298 K in fetal bovine serum (r(1) = 16.1 vs 8.5 mM(-1) s(-1) in HEPES buffer), due to hydrophobic interactions between the chelate and serum proteins. The isoquinoline core allows for the optimization of the optical properties of the luminescent lanthanide complexes in comparison to the pyridinic analogues and provides significant shifts of the excitation energies toward lower values which therefore become more adapted for biological applications. L2 and L3 bear two methoxy substituents on the aromatic core in ortho and para positions, respectively, that further modulate their electronic structure. The Nd(3+) and Yb(3+) complexes of the ligand L3, which incorporates the p-dimethoxyisoquinoline moiety, can be excited up to 420 nm. This wavelength is shifted over 100 nm toward lower energy in comparison to the pyridine-based analogue. The luminescence quantum yields of the Nd(3+) (0.013-0.016%) and Yb(3+) chelates (0.028-0.040%) are in the range of the best nonhydrated complexes, despite the presence of two inner sphere water molecules. More importantly, the 980 nm NIR emission band of YbL3 was detected with a good sensitivity in a proof of concept microscopy experiment at a concentration of 10 μM in fetal bovine serum. Our results demonstrate that even bishydrated NIR lanthanide complexes can emit a sufficient number of photons to ensure sensitive detection in practical applications. In particular, these ligands containing an aromatic core with coordinating pyridine nitrogen can be easily modified to tune the optical properties of the NIR luminescent lanthanide complexes while retaining good complex stability and MRI characteristics for the Gd(3+) analogues. They constitute a highly versatile platform for the development of bimodal MR and optical imaging probes based on a simple mixture of Gd(3+) and Yb(3+)/Nd(3+) complexes using an identical chelator. Given the presence of two inner sphere water molecules, important for MRI applications of the corresponding Gd(3+) analogues, this result is particularly exciting and opens wide perspectives not only for NIR imaging based on Ln(3+) ions but also for the design of combined NIR optical and MRI probes.
Published: 2012
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41. Functionalized single-walled carbon nanotubes containing traces of iron as new negative MRI contrast agents for in vivo imaging

Author: Doan , Bich-Thuy, Seguin , Johanne, Breton , Marie, Le Beherec , Ronan, Bessodes , Michel, Rodríguez-Manzo , Julio A, Banhart , Florian, Beloeil , Jean-Claude, Scherman , Daniel, Richard , Cyrille, Unité de pharmacologie chimique et génétique et d'imagerie (UPCGI - UMR 8151 / UMR_S 1022 ), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de physique et de chimie des matériaux (IPCM), Unité de pharmacologie chimique et génétique et d'imagerie ( UPCGI - UMR 8151 / UMR_S 1022 ), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de biophysique moléculaire ( CBM ), Université d'Orléans ( UO ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), and Institut de physique et de chimie des matériaux ( IPCM )
Subjects: MESH: Cell Death, Contrast Media, MESH : Iron, TOXICITY, MESH: Magnetic Resonance Imaging, X-Ray Diffraction, BIODISTRIBUTION, MAGNETIC-RESONANCE, MRI contrast agents, NANOPARTICLES, MESH: Animals, Lung, MESH: Iron, Cell Death, MESH : Hep G2 Cells, MESH: X-Ray Diffraction, Hep G2 Cells, Magnetic Resonance Imaging, CANCER, in vivo, Liver, MESH: Nanotubes, Carbon, MESH : Imaging, Three-Dimensional, functionalization, TOXICOLOGY, MESH : Lung, Iron, MESH: Hep G2 Cells, MESH: Imaging, Three-Dimensional, MESH : X-Ray Diffraction, Imaging, Three-Dimensional, MESH : Magnetic Resonance Imaging, MESH: Contrast Media, MESH : Mice, Animals, Humans, MESH: Lung, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Mice, MESH : Contrast Media, MESH: Humans, carbon nanotubes, Nanotubes, Carbon, MESH : Humans, MESH : Liver, TRANSPORTERS, GADONANOTUBES, MICE, MESH : Cell Death, traces of iron, ENHANCED MRI, MESH : Nanotubes, Carbon, MESH : Animals, MESH: Liver
Abstract: International audience; Single-walled carbon nanotubes (SWCNTs) containing traces of iron oxide were functionalized by noncovalent lipid-PEG or covalent carboxylic acid function to supply new efficient MRI contrast agents for in vitro and in vivo applications. Longitudinal (r(1)) and transversal (r(2)) water proton relaxivities were measured at 300 MHz, showing a stronger T(2) feature as an MRI contrast agent (r(2)/r(1) = 190 for CO(2) H functionalisation). The r(2) relaxivity was demonstrated to be correlated to the presence of iron oxide in the SWNT-carboxylic function COOH, in comparison to iron-free ones. Biodistribution studies on mice after a systemic injection showed a negative MRI contrast in liver, suggesting the presence of the nanotubes in this organ until 48 h after i.v. injection. The presence of carbon nanotubes in liver was confirmed after ex vivo carbon extraction. Finally, cytotoxicity studies showed no apparent effect owing to the presence of the carbon nanotubes. The functionalized carbon nanotubes were well tolerated by the animals at the dose of 10 µg g(-1) body weight.
Published: 2012
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42. How stereological analysis of vascular morphology can quantify the blood volume fraction as a marker for tumor vasculature: comparison with magnetic resonance imaging

Author: Perles-Barbacaru, Adriana, van Der Sanden, Boudewijn, Farion, Regine, Lahrech, Hana, Issartel, Jean-Paul, Inserm U836, équipe 7, Nanomédecine et cerveau, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), and The study was funded by PhD and post doc stipends from the French Cancer Research Association (ARC, Association pour la Recherche sur le Cancer) and the French National Cancer Institute(INCa, Institut National du Cancer).
Subjects: MESH: Blood Volume, MESH: Cell Line, Tumor, MESH: Rats, MESH: Heterocyclic Compounds, MESH: Blood Volume Determination, MESH: Microscopy, Fluorescence, [SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine, MESH: Models, Anatomic, [SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine, MESH: Magnetic Resonance Imaging, MESH: Glioma, vessel diameter, MESH: Contrast Media, MESH: Animals, MESH: Brain Mapping, MESH: Organometallic Compounds, MESH: Rats, Wistar, MESH: Image Processing, Computer-Assisted, vascular volume fraction, MESH: Microvessels, blood volume fraction, MESH: Brain Neoplasms, stereology, RSST1-MRI, MESH: Neovascularization, Pathologic, MESH: Neoplasm Transplantation
Abstract: International audience; To assess angiogenesis noninvasively in a C6 rat brain tumor model, the rapid-steady-state-T(1) (RSST(1)) magnetic resonance imaging (MRI) method was used for microvascular blood volume fraction (BVf) quantification with a novel contrast agent gadolinium per (3,6 anhydro) α-cyclodextrin (Gd-ACX). In brain tissue contralateral to the tumor, equal BVfs were obtained with Gd-ACX and the clinically approved gadoterate meglumine (Gd-DOTA). Contrary to Gd-DOTA, which leaks out of the tumor vasculature, Gd-ACX was shown to remain vascular in the tumor tissue allowing quantification of the tumor BVf. We sought to confirm the obtained tumor BVf using an independent method: instead of using a 'standard' two-dimensional histologic method, we study here how vascular morphometry combined with a stereological technique can be used for three-dimensional assessment of the vascular volume fraction (V(V)). The V(V) is calculated from the vascular diameter and length density. First, the technique is evaluated on simulated data and the healthy rat brain vasculature and is then applied to the same C6 tumor vasculature previously quantified by RSST(1)-MRI with Gd-ACX. The mean perfused V(V) and the BVf obtained by MRI in tumor regions are practically equal and the technique confirms the spatial heterogeneity revealed by MRI.
Published: 2012
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43. Effects of manganese injected into rat nostrils: implications for in vivo functional study of olfaction using MEMRI

Author: Gérard Coureaud, Benoist Lehallier, Yves Maurin, Jean-Marie Bonny, Structures tissulaires et interactions moléculaires (STIM), Institut National de la Recherche Agronomique (INRA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), UMR 1197 NOPA, Structures tissulaires et interactions moléculaires ( STIM ), Institut National de la Recherche Agronomique ( INRA ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and Neurobiologie de l'Olfaction et Modélisation en Imagerie (NOeMI)
Subjects: Male, Pathology, Brain activity and meditation, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, Contrast Media, Stimulation, Pharmacology, MESH : Behavior, Animal, MEMRI Manganese, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, MESH: Smell, MESH: Behavior, Animal, MESH: Animals, MESH: Administration, Intranasal, MESH : Olfactory Bulb, medicine.diagnostic_test, Behavior, Animal, Chemistry, MESH : Rats, Magnetic Resonance Imaging, Olfactory Bulb, Smell, Dose, Toxicity, MESH: Image Enhancement, MESH: Olfactory Bulb, medicine.medical_specialty, MESH: Rats, MESH : Male, Biomedical Engineering, Biophysics, MESH: Manganese, Olfaction, MESH : Rats, Wistar, 03 medical and health sciences, Primary olfactory cortex, In vivo, MESH : Magnetic Resonance Imaging, MESH: Contrast Media, medicine, Animals, Radiology, Nuclear Medicine and imaging, Rats, Wistar, Administration, Intranasal, MESH : Contrast Media, Behavior, Manganese, MESH : Administration, Intranasal, Magnetic resonance imaging, MESH: Rats, Wistar, Image Enhancement, MESH: Male, Rats, Odor, Rat, MESH : Smell, MESH : Image Enhancement, MESH : Animals, MESH : Manganese, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery
Abstract: WOS: 000298212500007; International audience; Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for visualizing neuronal pathways and mapping brain activity modulation. A potential drawback of MEMRI lies in the toxic effects of manganese (Mn), which also depend on its administration route. The aim of this study was to analyze the effects of Mn doses injected into the nostrils of rats on both olfactory perception and MRI contrast enhancement. For this purpose, doses in the range 0-8 μmol MnCl(2) were tested. Behavioral items were quantified with and without odor stimulation during the first 2 h following Mn injection. The MRI study was performed after 16 h of intermittent olfactory stimulations. Behavioral results showed that, during the early period following Mn administration, spontaneous motor activity was not affected, while odor-related behaviors were dose-dependently reduced. MRI results showed that, in the primary olfactory cortex, contrast was rapidly enhanced for Mn doses up to 0.3 μmol and very slowly above. This dose of 0.3 μmol Mn can thus be taken as the optimal dose for injection into rat nostrils to ensure a reproducible contrast in MRI studies while sparing olfactory perception.
Published: 2012
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44. Intracranial solitary fibrous tumor: imaging findings

Author: Jacques Chiras, Fabrice Bonneville, Frédéric Clarençon, Audrey Rousseau, Philippe Cornu, Damien Galanaud, Michèle Kujas, Olivier Naggara, Service de Neuroradiologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de neuroradiologie, CHU Toulouse [Toulouse], Service de neuro-pathologie [CHU Pitié-Salpêtrière], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Equipe NEMESIS - Centre de Recherches de l'Institut du Cerveau et de la Moelle épinière (NEMESIS-CRICM), Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de neuroradiologie [Paris], Hôpital Sainte-Anne, Service de Neurochirurgie [CHU Pitié-Salpêtrière], Equipe de Recherche Médicale Appliquée (ERMA), Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Université de Limoges (UNILIM)-CHU Limoges, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Pôle Cardiovasculaire et Métabolique [CHU Toulouse], and Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)
Subjects: In vivo magnetic resonance spectroscopy, Male, Solitary fibrous tumor, Magnetic Resonance Spectroscopy, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Gadolinium, MESH: Inositol, Contrast Media, Perfusion scanning, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, Choline, Diffusion, 0302 clinical medicine, Meninges, Heterocyclic Compounds, MESH: Neuroimaging, MESH: Middle Aged, medicine.diagnostic_test, Brain Neoplasms, MR spectroscopy, MESH: Choline, General Medicine, Middle Aged, Perfusion imaging, Magnetic Resonance Imaging, Solitary Fibrous Tumors, MESH: Brain Neoplasms, Female, Radiology, Tomography, MESH: Tomography, X-Ray Computed, MRI, Adult, medicine.medical_specialty, MESH: Heterocyclic Compounds, chemistry.chemical_element, Neuroimaging, MESH: Diffusion Magnetic Resonance Imaging, 03 medical and health sciences, MESH: Contrast Media, medicine, Organometallic Compounds, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Effective diffusion coefficient, Humans, Radiology, Nuclear Medicine and imaging, Retrospective Studies, MESH: Humans, business.industry, MESH: Magnetic Resonance Spectroscopy, Magnetic resonance imaging, MESH: Adult, MESH: Organometallic Compounds, MESH: Retrospective Studies, MESH: Solitary Fibrous Tumors, medicine.disease, MESH: Male, Diffusion Magnetic Resonance Imaging, chemistry, business, Tomography, X-Ray Computed, MESH: Female, 030217 neurology & neurosurgery, Inositol
Abstract: ERMA; International audience; OBJECTIVE: To study the neuroimaging features of intracranial solitary fibrous tumors (ISFTs). MATERIALS AND METHODS: Retrospective study of neuroimaging features of 9 consecutive histopathologically proven ISFT cases. Location, size, shape, density, signal intensity and gadolinium uptake were studied at CT and MRI. Data collected from diffusion-weighted imaging (DWI) (3 patients), perfusion imaging and MR spectroscopy (2 patients), and DSA (4 patients) were also analyzed. RESULTS: The tumors most frequently arose from the intracranial meninges (7/9), while the other lesions were intraventricular. Tumor size ranged from 2.5 to 10 cm (mean=6.6 cm). They presented multilobular shape in 6/9 patients. Most ISFTs were heterogeneous (7/9) with areas of low T2 signal intensity that strongly enhanced after gadolinium administration (6/8). Erosion of the skull was present in about half of the cases (4/9). Components with decreased apparent diffusion coefficient were seen in 2/3 ISFTs on DWI. Spectroscopy revealed elevated peaks of choline and myo-inositol. MR perfusion showed features of hyperperfusion. CONCLUSION: ISFT should be considered in cases of extra-axial, supratentorial, heterogeneous, hypervascular tumor. Areas of low T2 signal intensity that strongly enhance after gadolinium injection are suggestive of this diagnosis. Restricted diffusion and elevated peak of myo-inositol may be additional valuable features.
Published: 2011
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45. Unbinding of targeted ultrasound contrast agent microbubbles by secondary acoustic forces

Author: Detlef Lohse, M.L.J. Overvelde, Nico de Jong, Valeria Garbin, Michel Versluis, Benjamin Dollet, Institute for Biomedical Technology (MIRA), University of Twente [Netherlands], Institut de Physique de Rennes (IPR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), University of Twente, Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Cardiology, Faculty of Science and Technology, and Physics of Fluids
Subjects: Time Factors, Materials science, Surface Properties, Contrast Media, Nanotechnology, MESH: Ultrasonics, 01 natural sciences, High-Energy Shock Waves, Fluid dynamics, MESH: Contrast Media, 0103 physical sciences, Humans, Ultrasonics, Radiology, Nuclear Medicine and imaging, Acoustic radiation force, 010301 acoustics, Ultrasonography, 010302 applied physics, MESH: Surface Properties, Microbubbles, MESH: High-Energy Shock Waves, MESH: Humans, Radiological and Ultrasound Technology, business.industry, Cell Membrane, Ultrasound, Dynamics (mechanics), MESH: Time Factors, Adhesion, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, 2023 OA procedure, Acoustic radiation, Restoring force, Medical physics, business, Biological physics, MESH: Microbubbles, Biomedical engineering, MESH: Cell Membrane
Abstract: International audience; Targeted molecular imaging with ultrasound contrast agent microbubbles is achieved by incorporating targeting ligands on the bubble coating and allows for specific imaging of tissues affected by diseases. Improved understanding of the interplay between the acoustic forces acting on the bubbles during insonation with ultrasound and other forces (e.g. shear due to blood flow, binding of targeting ligands to receptors on cell membranes) can help improve the efficacy of this technique. This work focuses on the effects of the secondary acoustic radiation force, which causes bubbles to attract each other and may affect the adhesion of targeted bubbles. First, we examine the translational dynamics of ultrasound contrast agent microbubbles in contact with (but not adherent to) a semi-rigid membrane due to the secondary acoustic radiation force. An equation of motion that effectively accounts for the proximity of the membrane is developed, and the predictions of the model are compared with experimental data extracted from optical recordings at 15 million frames per second. A time-averaged model is also proposed and validated. In the second part of the paper, initial results on the translation due to the secondary acoustic radiation force of targeted, adherent bubbles are presented. Adherent bubbles are also found to move due to secondary acoustic radiation force, and a restoring force is observed that brings them back to their initial positions. For increasing magnitude of the secondary acoustic radiation force, a threshold is reached above which the adhesion of targeted microbubbles is disrupted. This points to the fact that secondary acoustic radiation forces can cause adherent bubbles to detach and alter the spatial distribution of targeted contrast agents bound to tissues during activation with ultrasound. While the details of the rupture of intermolecular bonds remain elusive, this work motivates the use of the secondary acoustic radiation force to measure the strength of adhesion of targeted microbubbles.
Published: 2011
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46. Dynamics of coated microbubbles adherent to a wall

Author: Michel Versluis, Detlef Lohse, M.L.J. Overvelde, Nico de Jong, Valeria Garbin, Benjamin Dollet, Institute for Biomedical Technology (MIRA), University of Twente, Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Faculty of Science and Technology, Physics of Fluids, University of Twente [Netherlands], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), and Cardiology
Subjects: Acoustics and Ultrasonics, Contrast Media, 02 engineering and technology, Adherent, Ligands, 030218 nuclear medicine & medical imaging, MESH: Fluorocarbons, 0302 clinical medicine, MESH: Nitrogen, Phospholipids, Targeting ligands, Fluorocarbons, Microbubbles, Radiological and Ultrasound Technology, Ultrasound, Dynamics (mechanics), Adhesion, Ultrasound contrast agents, 021001 nanoscience & nanotechnology, 3. Good health, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, 0210 nano-technology, Optoacoustic imaging, MESH: Transducers, Materials science, Nitrogen, Bubble, Transducers, Biophysics, Molecular imaging, Nanotechnology, Resonance, 03 medical and health sciences, Suspensions, MESH: Contrast Media, Radiology, Nuclear Medicine and imaging, MESH: Phospholipids, Targeting, MESH: Molecular Imaging, business.industry, Nonlinear bubble dynamics, Acoustics, MESH: Suspensions, MESH: Acoustics, 2023 OA procedure, business, MESH: Microbubbles, Biomedical engineering
Abstract: Molecular imaging with ultrasound is a promising noninvasive technique for disease-specific imaging, enabling for instance, the diagnosis of thrombus and inflammation. Selective imaging is performed by using ultrasound contrast agent microbubbles functionalized with ligands, which bind specifically to the target molecules. Here, we investigate in a model system, the influence of adherence at a wall on the dynamics of the microbubbles, in particular, on the frequency of maximum response, by recording the radial response of individual microbubbles as a function of the applied acoustic pressure and frequency. The frequency of maximum response of adherent microbubbles was found to be over 50% lower than for bubbles in the unbounded fluid and over 30% lower than that of a nonadherent bubble in contact with the wall. The change is caused by adhesion of the bubbles to the wall as no influence was found due solely to the presence of the targeting ligands on the bubble dynamics. The shift in the frequency of maximum response may prove to be important for molecular imaging with ultrasound as this application would benefit from an acoustic imaging method to distinguish adherent microbubbles from freely circulating microbubbles. (E-mail: m.versluis@utwente.nl) (C) 2011 World Federation for Ultrasound in Medicine & Biology.
Published: 2011
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47. Improved diagnosis of post-operative myocardial infarction by contrast echocardiography after coronary artery bypass graft surgery

Author: Gaelle Kervio, Anne Bernard, Hervé Le Breton, Frédéric Schnell, Christophe Thebault, Erwan Flecher, Bernard Lelong, Alain Leguerrier, Hervé Corbineau, Erwan Donal, Service de cardiologie et maladies vasculaires [Rennes] = Cardiac, Thoracic, and Vascular Surgery [Rennes], CHU Pontchaillou [Rennes], Service de chirurgie thoracique cardiaque et vasculaire [Rennes] = Thoracic and Cardiovascular Surgery [Rennes], Service de cardiologie et maladies vasculaires [Rennes], Service de chirurgie thoracique cardiaque et vasculaire [Rennes], Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes]
Subjects: Male, Time Factors, MESH: Chi-Square Distribution, Statistics as Topic, Myocardial Infarction, Contrast Media, 030204 cardiovascular system & hematology, Postoperative Complications, 0302 clinical medicine, MESH: Postoperative Complications, Troponin I, Medicine, Contrast (vision), 030212 general & internal medicine, Myocardial infarction, Coronary Artery Bypass, Post operative, Ultrasonography, media_common, MESH: Aged, Ejection fraction, General Medicine, MESH: Myocardial Infarction, medicine.anatomical_structure, Post-operative myocardial infarction, Cardiology, Female, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Cardiology and Cardiovascular Medicine, Cardiac wall motion, Artery, medicine.medical_specialty, MESH: Hemodynamics, Contrast enhancement, Heart Ventricles, media_common.quotation_subject, 03 medical and health sciences, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, MESH: Contrast Media, Contrast echocardiography, Humans, Radiology, Nuclear Medicine and imaging, MESH: Statistics as Topic, Aged, Chi-Square Distribution, MESH: Humans, business.industry, MESH: Coronary Artery Bypass, MESH: Time Factors, Hemodynamics, MESH: Biological Markers, medicine.disease, MESH: Male, Surgery, MESH: Heart Ventricles, business, MESH: Female, Biomarkers
Abstract: International audience; OBJECTIVE: Contrast echocardiography is a more accurate means of assessment of left ventricular (LV) regional motion compared with non-enhanced echocardiography. Despite new tests, the diagnosis of post-operative myocardial infarction (MI) remains difficult. The aim of this study was to determine whether contrast echocardiography can facilitate this diagnosis. METHODS: We performed standard and contrast echocardiography in 79 consecutive patients before and up to 10 days after they underwent isolated coronary artery bypass graft (CABG) surgery. RESULTS: The post-operative intra- and interobserver reproducibility of echocardiographic measurements of the LV ejection fraction and wall motion score was significantly higher with than without contrast enhancement. The proportion of non-visualized LV myocardial segments was significantly greater with non-enhanced (6.6%) than with contrast (0.3%) echocardiography (P< 0.0001). The mean peak serum cardiac troponin (cTnI) concentration was significantly higher in the group of patients with new wall motion abnormalities detected with contrast-enhanced echocardiography. A correlation was found (r = -0.43, P< 0.01) between peak cTnI and changes in wall motion score only when a contrast agent was used. CONCLUSION: Contrast echocardiography facilitated the detection of new LV wall motion abnormalities after CABG. This observation, added to other markers, might facilitate the diagnosis of post-operative MI.
Published: 2011
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48. Overestimation of moderate carotid stenosis assessed by both Doppler US and contrast enhanced 3D-MR angiography in the CARMEDAS study

Author: A. Pasco-Papon, Jean-Yves Gauvrit, Jean Michel Serfaty, Philippe Douek, Michel Nonent, J.-O. Fortrat, X. Papon, Valérie Buthion, C. Pachai, Y. Le Bras, A Badra, Luc Bressollette, D. Ben Salem, J.-P. Cottier, C. Rotaru, Jérôme Berge, Pierre Gouny, Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Département de radiologie [Brest] (DR - Brest), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Département de Radiologie [Bichat] (DR- Bichat), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Health System Analysis Laboratory (GATE), Université de Lyon, Département de Neuro-Radiologie [Angers] (DNR - Angers), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Département de Médecine Interne et Pneumologie [Brest] (DMIP - Brest), Département de Chirurgie Vasculaire [Angers] (DCV - Angers), Bioclinica SAS (B SAS), Bioclinica SAS, Département d'Angiologie [Angers] (DA - Angers), Service de chirurgie thoracique et cardio vasculaire [Brest] (CCTV - Brest), Département de Neuro-Radiologie [Bordeaux] (DNR - Bordeaux), CHU Bordeaux [Bordeaux], Département de Radiologie [Niort] (DR - Niort), CH Niort, Vision, Action et Gestion d'informations en Santé (VisAGeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE (IRISA-D5), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Télécom Bretagne-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Service de radiologie et imagerie médicale [Rennes] = Radiology [Rennes], CHU Pontchaillou [Rennes], Imagerie et modélisation Vasculaires, Thoraciques et Cérébrales (MOTIVATE), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Télécom Bretagne-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Université de Bretagne Sud (UBS)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), and Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon)
Subjects: Male, Contrast Media, MESH: Magnetic Resonance Angiography, Magnetic resonance angiography, 030218 nuclear medicine & medical imaging, 0302 clinical medicine, MESH: Aged, 80 and over, MESH: Carotid Stenosis, Contrast (vision), Carotid Stenosis, media_common, Aged, 80 and over, MESH: Aged, MESH: Middle Aged, Radiological and Ultrasound Technology, medicine.diagnostic_test, Middle Aged, MESH: Predictive Value of Tests, Predictive value of tests, cardiovascular system, Female, Radiology, medicine.medical_specialty, media_common.quotation_subject, MESH: Imaging, Three-Dimensional, Sensitivity and Specificity, 03 medical and health sciences, Imaging, Three-Dimensional, Predictive Value of Tests, MESH: Contrast Media, Image Interpretation, Computer-Assisted, medicine, Humans, Radiology, Nuclear Medicine and imaging, cardiovascular diseases, Aged, MESH: Humans, business.industry, Mr angiography, Ultrasonography, Doppler, Digital subtraction angiography, medicine.disease, MESH: Sensitivity and Specificity, MESH: Male, Stenosis, Angiography, MESH: Ultrasonography, Doppler, Neurology (clinical), Doppler ultrasound, business, MESH: Female, MESH: Image Interpretation, Computer-Assisted, Magnetic Resonance Angiography, 030217 neurology & neurosurgery, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; PURPOSE: To evaluate the agreement and diagnostic accuracy of Contrast enhanced magnetic resonance angiography (CE-MRA), Doppler ultrasound (DUS) and Digital subtraction angiography (DSA) in the assessment of carotid stenosis. METHODS: DUS, CE-MRA and DSA were performed in 56 patients included in the Carotide-angiographie par résonance magnétique-échographie-doppler-angioscanner (CARMEDAS) multicenter study with a carotid stenosis ≥ 50%. Three readers evaluated stenoses on CE-MRA and DSA (NASCET criteria). Velocities criteria were used for stenosis estimation on DUS. RESULTS: CE-MRA had a sensitivity and specificity of 96-98% and 66-83% respectively for carotid stenoses ≥ 50% and a sensitivity and specificity of 94% and 76-84% respectively for carotid stenoses ≥ 70%. The interobserver agreement of CE-MRA was excellent, except for moderate stenoses (50-69%). DUS had a sensitivity and specificity of 88 and 75% respectively for carotid stenoses ≥ 50% and a sensitivity and specificity of 83 and 86% respectively for carotid stenoses ≥ 70%. Combined concordant CE-MRA and DUS had a sensitivity and specificity of 100 and 85-90% respectively for carotid stenoses ≥ 50% and a sensitivity and specificity of 96-100% and 80-87% respectively for carotid stenoses ≥ 70%. The positive predictive value of the association CE-MRA and DUS for carotid stenoses ≥ 70% is calculated between 77 and 82% while the negative predictive value is calculated between 97 and 100%. CE-MRA and DUS have concordant findings in 63-72%, and the overestimations cases were recorded only for carotid stenosis ≤ 69%. CONCLUSION: Combined DUS-CE-MRA is excellent for evaluation of severe stenosis but remains debatable in moderate stenosis (50-69%) due to the risk of overestimations.
Published: 2011
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49. Influence of Calcium-Induced Aggregation on the Sensitivity of Aminobis(methylenephosphonate)-Containing Potential MRI Contrast Agents

Author: I Mamedov, Hermann A. Mayer, Nikos K. Logothetis, Goran Angelovski, Éva Tóth, Petra Fousková, Jörg Henig, Max Planck Institute for Biological Cybernetics, Max-Planck-Gesellschaft, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Physiology of Cognitive Processes
Subjects: Lanthanide, IONS, Magnetic Resonance Spectroscopy, RELAXIVITY, Potentiometric titration, Inorganic chemistry, RESONANCE-IMAGING AGENTS, MESH: Molecular Structure, Contrast Media, Protonation, 010402 general chemistry, MESH: Chelating Agents, 01 natural sciences, Medicinal chemistry, Lanthanoid Series Elements, MESH: Magnetic Resonance Imaging, Inorganic Chemistry, chemistry.chemical_compound, MESH: Lanthanoid Series Elements, LANTHANIDE COMPLEXES, MESH: Contrast Media, MAGNETIC-RESONANCE, Side chain, Organometallic Compounds, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Physical and Theoretical Chemistry, Alkyl, Chelating Agents, chemistry.chemical_classification, Molecular Structure, 010405 organic chemistry, Ligand, DERIVATIVES, MESH: Magnetic Resonance Spectroscopy, MESH: Organometallic Compounds, Stereoisomerism, Nuclear magnetic resonance spectroscopy, PGSE DIFFUSION MEASUREMENTS, ACIDS, Phosphonate, MESH: Stereoisomerism, Magnetic Resonance Imaging, NMR, 0104 chemical sciences, chemistry, MESH: Calcium, LUMINESCENCE, Calcium
Abstract: International audience; A novel class of 1,4,7,10-tetraazacyclododecane-1,4,7-tris(methylenecarboxylic) acid (DO3A)-based lanthanide complexes with relaxometric response to Ca(2+) was synthesized, and their physicochemical properties were investigated. Four macrocyclic ligands containing an alkyl-aminobis(methylenephosphonate) side chain for Ca(2+)-chelation have been studied (alkyl is propyl, butyl, pentyl, and hexyl for L(1), L(2), L(3), and L(4), respectively). Upon addition of Ca(2+), the r(1) relaxivity of their Gd(3+) complexes decreased up to 61% of the initial value for the best compounds GdL(3) and GdL(4). The relaxivity of the complexes was concentration dependent (it decreases with increasing concentration). Diffusion NMR studies on the Y(3+) analogues evidenced the formation of agglomerates at higher concentrations; the aggregation becomes even more important in the presence of Ca(2+). (31)P NMR experiments on EuL(1) and EuL(4) indicated the coordination of a phosphonate to the Ln(3+) for the ligand with a propyl chain, while phosphonate coordination was not observed for the analogue bearing a hexyl linker. Potentiometric titrations yielded protonation constants of the Gd(3+) complexes. log K(H1) values for all complexes lie between 6.12 and 7.11 whereas log K(H2) values are between 4.61 and 5.87. Luminescence emission spectra recorded on the Eu(3+) complexes confirmed the coordination of a phosphonate group to the Ln(3+) center in EuL(1). Luminescence lifetime measurements showed that Ca-induced agglomeration reduces the hydration number which is the main cause for the change in r(1). Variable temperature (17)O NMR experiments evidenced high water exchange rates on GdL(1), GdL(2), and GdL(3) comparable to that of the aqua ion.
Published: 2011
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50. Nonlinear shell behavior of phospholipid-coated microbubbles

Author: Valeria Garbin, M.L.J. Overvelde, Benjamin Dollet, Michel Versluis, Nico de Jong, Jeroen Sijl, Detlef Lohse, Institute for Biomedical Technology (MIRA), University of Twente, Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Cardiology, Physics of Fluids, Faculty of Science and Technology, University of Twente [Netherlands], Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Acoustics and Ultrasonics, Acoustics, Bubble, Biophysics, Shell (structure), Contrast Media, Viscoelastic Substances, MESH: Ultrasonics, 01 natural sciences, Resonance, Viscoelasticity, 030218 nuclear medicine & medical imaging, Physics::Fluid Dynamics, 03 medical and health sciences, 0302 clinical medicine, Coated Materials, Biocompatible, MESH: Coated Materials, Biocompatible, MESH: Contrast Media, 0103 physical sciences, Phospholipid coating, Ultrasonics, Radiology, Nuclear Medicine and imaging, MESH: Viscoelastic Substances, Sound pressure, 010301 acoustics, Phospholipids, Ultrasonography, MESH: Phospholipids, Microbubbles, Radiological and Ultrasound Technology, Nonlinear bubble dynamics, Mechanics, Ultrasound contrast agents, 3. Good health, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Nonlinear system, IR-73962, Buckling, METIS-272789, MESH: Microbubbles, MESH: Ultrasonography
Abstract: The influence of the stabilizing phospholipid-coating on the nonlinear dynamics of ultrasound contrast agent microbubbles is investigated. We record the radial dynamics of individual microbubbles with an ultra high-speed camera as a function of both driving pressure and frequency. The viscoelastic shell was found to enhance the nonlinear bubble response at acoustic pressures as low as 10 kPa. For increasing acoustic pressures a decrease of the frequency of maximum response was observed for a distinct class of bubbles, leading to a pronounced skewness of the resonance curve, which we show to be the origin of the "thresholding" behavior (Emmer et al. 2007). For the other bubbles, the frequency of maximum response was found to lie just above the resonance frequency of an uncoated microbubble and to be independent of the applied acoustic pressure. The shell-buckling bubble model (Marmottant et al. 2005), which accounts for buckling and rupture of the shell, captures both cases for a unique set of the shell parameters, the relevant parameter being the phospholipid concentration at the bubble interface. (E-mail: m.versluis@utwente.nl) (C) 2010 World Federation for Ultrasound in Medicine & Biology.
Published: 2010
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