1. Cellular localization of apelin and its receptor in the anterior pituitary: evidence for a direct stimulatory action of apelin on ACTH release
- Author
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Marie-Thérèse Bluet-Pajot, Rodrigo Alvear-Perez, Philippe Zizzari, Catherine Llorens-Cortes, Annabelle Réaux-Le Goazigo, Jacques Epelbaum, Neuropeptides centraux et régulations hydrique et cardiovasculaire, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Labex MemoLife, Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neurobiologie de la Croissance et de la Senescence, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre interdisciplinaire de recherche en biologie (CIRB), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Labex MemoLife, Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Collège de France ( CdF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), and Llorens Cortes, Catherine
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Male ,Pituitary gland ,Endocrinology, Diabetes and Metabolism ,MESH: Amino Acid Sequence ,MESH: Receptors, G-Protein-Coupled ,MESH: Base Sequence ,MESH : Tissue Distribution ,Receptors, G-Protein-Coupled ,Rats, Sprague-Dawley ,0302 clinical medicine ,MESH: Cricetulus ,MESH: Pituitary Gland ,MESH: Ligands ,MESH : CHO Cells ,Tissue Distribution ,MESH: Animals ,MESH: Peptide Fragments ,MESH : Ligands ,0303 health sciences ,MESH : Amino Acid Sequence ,MESH : Cyclic AMP ,MESH: Pituitary Gland, Anterior ,MESH : Cricetinae ,MESH : Protein Binding ,[ SDV.MHEP.EM ] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,Apelin ,MESH : Adrenocorticotropic Hormone ,Intercellular Signaling Peptides and Proteins ,MESH : Carrier Proteins ,medicine.medical_specialty ,endocrine system ,MESH : Cell Membrane ,MESH : Cricetulus ,MESH: Carrier Proteins ,Adrenocorticotropic hormone ,03 medical and health sciences ,Adrenocorticotropic Hormone ,Anterior pituitary ,Pituitary Gland, Anterior ,MESH: CHO Cells ,Physiology (medical) ,MESH: Protein Binding ,MESH: Tissue Distribution ,MESH: Adrenocorticotropic Hormone ,MESH: Humans ,MESH: Molecular Sequence Data ,MESH : Humans ,Peptide Fragments ,Endocrinology ,Carrier Proteins ,030217 neurology & neurosurgery ,MESH : Molecular Sequence Data ,Physiology ,MESH: Corticotrophs ,MESH: Cricetinae ,MESH : Models, Biological ,MESH: Rats, Sprague-Dawley ,Rats, Inbred WKY ,MESH : Pituitary Gland ,Receptor ,Corticotrophs ,Cellular localization ,MESH: Cyclic AMP ,[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,Apelin Receptors ,MESH : Rats ,MESH : Forskolin ,medicine.anatomical_structure ,MESH: Pituitary Hormones, Anterior ,MESH: Calcium ,MESH : Receptors, G-Protein-Coupled ,MESH : Transfection ,MESH: Forskolin ,hormones, hormone substitutes, and hormone antagonists ,MESH: Rats, Inbred WKY ,Endocrine gland ,MESH: Rats ,MESH : Male ,MESH : Rats, Inbred WKY ,Biology ,Models, Biological ,MESH : Pituitary Hormones, Anterior ,MESH : Pituitary Gland, Anterior ,Pituitary Hormones, Anterior ,Internal medicine ,medicine ,Animals ,MESH : Calcium ,030304 developmental biology ,Apelin receptor ,MESH: Transfection ,MESH : Peptide Fragments ,MESH: Models, Biological ,MESH : Corticotrophs ,MESH : Rats, Sprague-Dawley ,MESH: Male ,Rats ,MESH : Peptide Hormones ,MESH: Peptide Hormones ,MESH : Base Sequence ,MESH : Animals ,MESH: Cell Membrane - Abstract
Apelin is a bioactive peptide recently identified as the endogenous ligand of the human orphan G protein-coupled receptor APJ. The presence of apelin-immunoreactive nerve fibers, together with the detection of apelin receptor mRNA in the parvocellular part of the paraventricular nucleus and the stimulatory action of apelin on corticotropin-releasing hormone release, indicate that apelin modulates adrenocorticotropin (ACTH) release via an indirect action on the hypothalamus. However, a direct action of apelin in the anterior pituitary cannot be excluded. Here, we provided evidence for the existence of an apelinergic system within the adult male rat pituitary gland. Double immunofluorescence staining indicated that apelin is highly coexpressed in the anterior pituitary, mainly in corticotrophs (96.5 ± 0.3%) and to a much lower extent in somatotropes (3.2 ± 0.2%). Using in situ hybridization combined with immunohistochemistry, a high expression of apelin receptor mRNA was also found in corticotrophs, suggesting a local interaction between apelin and ACTH. In an ex vivo perifusion system of anterior pituitaries, apelin 17 (K17F, 10−6M) significantly increased basal ACTH release by 41%, whereas apelin 10 (R10F, 10−6M), an inactive apelin fragment, was ineffective. In addition, K17F but not R10F induced a dose-dependent increase in K+-evoked ACTH release, with maximal increase being observed for a 10−6M concentration. Taken together, these data outline the potential role of apelin as an autocrine/paracrine-acting peptide on ACTH release and provide morphological and neuroendocrine basis for further studies that explore the physiological role of apelin in the regulation of anterior pituitary functions.
- Published
- 2007
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