1. Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers
- Author
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Stéphane Bouchonnet, Nicolas Creusot, Jean-Marc Porcher, Sophie Bourcier, Said Kinani, Patrick Balaguer, Selim Ait-Aissa, Institut National de l'Environnement Industriel et des Risques (INERIS), Département de Chimie de l'École polytechnique (X-DEP-CHIM), École polytechnique (X), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Laboratoire des mécanismes réactionnels (DCMR), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), This study was funded by the French Ministry of Ecology and Sustainable Development (Program 189), the 'Agence Française de Sécurité Sanitaire de l'Environnement et du Travail' (AFSSET, RD-2005-02) and by a doctoral fellowship from the ANRT and INERIS (to SK)., and Aït-Aïssa, Selim
- Subjects
[SDE] Environmental Sciences ,Geologic Sediments ,Receptors, Steroid ,Bisphenol A ,Health, Toxicology and Mutagenesis ,0211 other engineering and technologies ,02 engineering and technology ,Endocrine Disruptors ,010501 environmental sciences ,Toxicology ,SEDIMENT ,01 natural sciences ,chemistry.chemical_compound ,Bioassay ,media_common ,MESH: Dioxins ,Pregnane ,Pregnane X Receptor ,General Medicine ,Contamination ,Pollution ,MESH: Endocrine Disruptors ,[SDV.TOX] Life Sciences [q-bio]/Toxicology ,MESH: Water Pollutants, Chemical ,Receptors, Estrogen ,Endocrine disruptor ,Receptors, Androgen ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Environmental chemistry ,[SDE]Environmental Sciences ,MESH: Receptors, Androgen ,MESH: Receptors, Estrogen ,Biological Assay ,MESH: Environmental Monitoring ,Environmental Monitoring ,media_common.quotation_subject ,[SDE.MCG]Environmental Sciences/Global Changes ,BIOESSAIS IN VITRO ,Estrone ,Dioxins ,MESH: Biological Assay ,MESH: Rivers ,Rivers ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,14. Life underwater ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,Persistent organic pollutant ,MESH: Geologic Sediments ,COURS D'EAU ,Receptors, Aryl Hydrocarbon ,chemistry ,13. Climate action ,MESH: Receptors, Aryl Hydrocarbon ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Water Pollutants, Chemical ,MESH: Receptors, Steroid - Abstract
International audience; A comprehensive evaluation of organic contamination was performed in sediments sampled in two reference and three impacted small streams where endocrine disruptive (ED) effects in fish have been evidenced. The approach combined quantitative chemical analyses of more than 50 ED chemicals (EDCs) and a battery of in vitro bioassays allowing the quantification of receptor-mediated activities, namely estrogen (ER), androgen (AR), dioxin (AhR) and pregnane X (PXR) receptors. At the most impacted sites, chemical analyses showed the presence of natural estrogens, organochlorine pesticides, parabens, polycyclic aromatic hydrocarbons (16 PAHs), bisphenol A and alkylphenols, while synthetic steroids, myco-estrogens and phyto-estrogens were not detected. Determination of toxic-equivalent amounts showed that 28-96% of estrogenic activities in bioassays (0.2-6.3 ng/g 17beta-estradiol equivalents) were explained by 17beta-estradiol and estrone. PAHs were major contributors (20-60%) to the total dioxin-like activities. Interestingly, high PXR and (anti)AR activities were detected; however, the targeted analysed compounds could not explain the measured biological activities. This study highlighted the presence of multiple organic EDCs in French river sediments subjected to mixed diffuse pollution, and argues for the need to further identify AR and PXR active compounds in the aquatic environment.
- Published
- 2010