1. Chikungunya Virus Replication in Salivary Glands of the Mosquito Aedes albopictus
- Author
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Christine Schmitt, Jacomine Krijnse Locker, Anubis Vega-Rúa, Isabelle Bonne, Anna-Bella Failloux, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Microscopie ultrastructurale - Ultrapole (CITECH), This study was funded by the Institut Pasteur, the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant No. ANR-10-LABX-62-IBEID). AVR was supported by the French Ministry of Superior Education and Research., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
- Subjects
Saliva ,replication ,Aedes albopictus ,viruses ,salivary glands ,lcsh:QR1-502 ,budding ,MESH: Chikungunya virus/physiology ,medicine.disease_cause ,Virus Replication ,Arbovirus ,Virus ,lcsh:Microbiology ,MESH: Chikungunya virus/growth & development ,Microscopy, Electron, Transmission ,stomatognathic system ,Aedes ,Virology ,parasitic diseases ,transmission electron microscopy ,medicine ,Animals ,MESH: Animals ,Chikungunya ,biology ,MESH: Aedes/virology ,Brief Report ,fungi ,MESH: Virus Replication ,Virion ,virus diseases ,Midgut ,MESH: Salivary Glands/virology ,biology.organism_classification ,medicine.disease ,3. Good health ,Infectious Diseases ,Viral replication ,MESH: Virion/ultrastructure ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,MESH: Microscopy, Electron, Transmission ,Female ,Chikungunya virus ,MESH: Female - Abstract
International audience; Chikungunya virus (CHIKV) is an emerging arbovirus transmitted to humans by mosquitoes such as Aedes albopictus. To be transmitted, CHIKV must replicate in the mosquito midgut, then disseminate in the hemocele and infect the salivary glands before being released in saliva. We have developed a standardized protocol to visualize viral particles in the mosquito salivary glands using transmission electron microscopy. Here we provide direct evidence for CHIKV replication and storage in Ae. albopictus salivary glands.
- Published
- 2017
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