1. Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector
- Author
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Karima, Zouache, Rory J, Michelland, Anna-Bella, Failloux, Genevieve L, Grundmann, Patrick, Mavingui, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Arbovirus et Insectes Vecteurs, Institut Pasteur [Paris], KZ was supported by PhD fellowships from the French Ministère de l’Education Nationale, de la Recherche et des Nouvelles Technologies. We thank Martina Kyselková and Juliana Almario for advices on microarray experiments. We thank Laurence Mousson and Camilo Arias-Goeta for mosquito’s infection. This work was funded by grants from Agence National de la Recherche (ANR-06-SEST07 ChikVendoM) and Fondation pour la Recherche sur la Biodiversité (FRB, formely IFB, CD-AOOI-07-012) and was carried out within the frameworks of GDRI ‘Biodiversité et Développement Durable à Madagascar’ and COST action F0701 ‘Arthropod Symbioses: from fundamental to pest disease management’., ANR-06-SEST-0007,CHIKVENDOM,Interactions multipartites entre le virus chikungunya, les endosymbiostes et les moustiques. Impact sur la transmission virale et la dynamique des populations vectorielles ( 2006 ), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, ANR-06-SEST-0007,CHIKVENDOM,Interactions multipartites entre le virus chikungunya, les endosymbiostes et les moustiques. Impact sur la transmission virale et la dynamique des populations vectorielles(2006), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)
- Subjects
chikungunya ,OUTBREAK ,MESH: Alphaproteobacteria ,viruses ,MESH : Symbiosis ,Virus Replication ,bacterial community ,Aedes ,MESH : Insect Vectors ,INFECTION ,MESH : Biodiversity ,WOLBACHIA ,MESH: Animals ,MESH : Host-Pathogen Interactions ,MESH: Symbiosis ,virus diseases ,Biodiversity ,MESH: Aedes ,MESH : Enterobacteriaceae ,Aedes albopictus ,MESH : Alphaproteobacteria ,MESH : Bacterial Physiological Phenomena ,MESH : Virus Replication ,REUNION ISLAND ,MESH: Gammaproteobacteria ,MESH : Wolbachia ,Host-Pathogen Interactions ,TAXONOMIC MICROARRAY ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,POPULATIONS ,MESH: Wolbachia ,Chikungunya virus ,microarray ,Gammaproteobacteria ,MESH : Bacteroidetes ,DENGUE ,TRANSMISSION ,MESH: Insect Vectors ,MESH : Chikungunya virus ,Bacterial Physiological Phenomena ,[ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,MESH: Biodiversity ,AEGYPTI ,MESH: Enterobacteriaceae ,Enterobacteriaceae ,MESH : Gammaproteobacteria ,parasitic diseases ,Animals ,Symbiosis ,Alphaproteobacteria ,MESH : Aedes ,Bacteroidetes ,MESH: Virus Replication ,MESH: Host-Pathogen Interactions ,fungi ,MESH: Chikungunya virus ,AEDES-ALBOPICTUS ,MESH: Bacteroidetes ,Insect Vectors ,MESH: Bacterial Physiological Phenomena ,quantitative PCR ,MESH : Animals - Abstract
International audience; Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However, there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha-and Gammaproteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection, whereas the abundance of known insect endosymbionts like Wolbachia and Blattabacterium decreased. Our results clearly link the pathogen propagation with changes in the dynamics of the bacterial community, suggesting that cooperation or competition occurs within the host, which may in turn affect the mosquito traits like vector competence.
- Published
- 2012
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