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5 results on '"MESH : Integrons"'

1. IMP-29, a Novel IMP-Type Metallo-β-Lactamase in Pseudomonas aeruginosa

Author

Patrick Plésiat, Marjorie Robert-Nicoud, Pascal Cholley, Katy Jeannot, Laurent Poirel, Patrice Nordmann, Résistances émergentes aux antibiotiques, Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Hôpital Jean Minjoz, Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Hôpital de Bicêtre, Agents pathogènes et inflammation - UFC (EA 4266) ( API ), Université de Franche-Comté ( UFC ), Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Agents pathogènes et inflammation - UFC (EA 4266) (API), and Université de Franche-Comté (UFC)

Subjects
Male, MESH: Fatal Outcome, MESH : Escherichia coli, MESH : Aged, MESH: beta-Lactamases, MESH : Carbapenems, medicine.disease_cause, Integron, Polymerase Chain Reaction, Integrons, law.invention, MESH: Carbapenems, chemistry.chemical_compound, Fatal Outcome, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, law, polycyclic compounds, MESH : DNA, Bacterial, Pharmacology (medical), MESH : Anti-Bacterial Agents, MESH : Polymerase Chain Reaction, Polymerase chain reaction, MESH: Aged, chemistry.chemical_classification, MESH: Microbial Sensitivity Tests, 0303 health sciences, Strain (chemistry), MESH: Escherichia coli, Hydrolysis, MESH : beta-Lactamases, MESH: Pseudomonas Infections, MESH : Pseudomonas aeruginosa, MESH : Pseudomonas Infections, Shock, Septic, Anti-Bacterial Agents, MESH: Integrons, 3. Good health, Amino acid, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, Gene cassette, Pseudomonas aeruginosa, MESH: Pseudomonas aeruginosa, MESH: Hydrolysis, DNA, Bacterial, MESH : Integrons, MESH : Male, MESH: Shock, Septic, Microbial Sensitivity Tests, Biology, beta-Lactamases, Microbiology, Bacterial genetics, 03 medical and health sciences, MESH : Shock, Septic, MESH : Hydrolysis, Mechanisms of Resistance, MESH: Anti-Bacterial Agents, Escherichia coli, medicine, Humans, Pseudomonas Infections, Aged, 030304 developmental biology, Pharmacology, MESH: Humans, 030306 microbiology, MESH : Humans, MESH: Polymerase Chain Reaction, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, MESH: DNA, Bacterial, MESH: Male, enzymes and coenzymes (carbohydrates), Carbapenems, MESH : Fatal Outcome, chemistry, biology.protein, bacteria, MESH : Microbial Sensitivity Tests, DNA
Abstract

Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The bla IMP-29 gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems.

Published
2012
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2. VEB-1 in Achromobacter xylosoxidans from Cystic Fibrosis Patient, France

Author

Eliane Siebor, Carine Freby, André Pechinot, Frédéric Huet, Agathe Ogier-Desserrey, Anne Houzel, Stéphanie Perez-Martin, Jean-Marie Duez, Catherine Neuwirth, UFR des Sciences de Santé (Université de Bourgogne), Université de Bourgogne ( UB ), Laboratoire de Microbiologie Médicale et Moléculaire, Laboratoire de Bactériologie (CHU Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre de génétique - Centre de référence des maladies rares, anomalies du développement et syndromes malformatifs (CHU de Dijon), Humbert, Murielle, Université de Bourgogne (UB), Laboratoire de bactériologie (CHU Dijon), Plateau technique de Biologie [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)

Subjects
Male, MESH : Molecular Sequence Data, integron, MESH: beta-Lactamases, lcsh:Medicine, MESH: Base Sequence, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Integron, Cystic fibrosis, Integrons, cystic fibrosis, ComputingMilieux_MISCELLANEOUS, MESH: Microbial Sensitivity Tests, 0303 health sciences, education.field_of_study, biology, Escherichia coli Proteins, MESH : beta-Lactamases, Achromobacter denitrificans, dispatch, Achromobacter xylosoxidans, MESH: Integrons, 3. Good health, MESH : Achromobacter denitrificans, France, medicine.symptom, MESH : Integrons, MESH: Cystic Fibrosis, Adolescent, MESH : Male, Molecular Sequence Data, Population, Microbial Sensitivity Tests, beta-Lactamases, Microbiology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, MESH : Adolescent, MESH : Cystic Fibrosis, medicine, Humans, Base sequence, lcsh:RC109-216, education, 030304 developmental biology, MESH: Adolescent, MESH: Humans, MESH: Molecular Sequence Data, Base Sequence, 030306 microbiology, business.industry, MESH : Humans, lcsh:R, Extended-spectrum beta-lactamase VEB-1, biology.organism_classification, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Male, MESH: Achromobacter denitrificans, biology.protein, Sputum, MESH : Base Sequence, MESH : Microbial Sensitivity Tests, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, business
Abstract

Multidrug-resistant Achromobacter xylosoxidans was recovered from the sputum of a patient with cystic fibrosis. The VEB-1 extended-spectrum β-lactamase was detected on a class 1 integron. This first report of a VEB-1–producing isolate in this population requires further investigation to determine its distribution.

Published
2006

3. Evidence for induction of integron-based antibiotic resistance by the SOS response in a clinical setting

Author

Didier Hocquet, Samuel I. Miller, Michelle Thouverez, Didier Mazel, Catherine Llanes, Hemantha D. Kulasekara, Xavier Bertrand, Patrick Plésiat, Agents pathogènes et inflammation - UFC (EA 4266) (API), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Department of Immunology, Medicine and Microbiology, University of Washington [Seattle], Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Plasticité du Génome Bactérien (PGB), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), National Institute of Allergy and Infectious Diseases (NIH grants 10265SUB and U54AI057141), Agents pathogènes et inflammation - UFC (EA 4266) ( API ), Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Hôpital Jean Minjoz, Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Plasticité du Génome Bactérien ( PGB ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement (UMR 6249) (LCE), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Bacterial Diseases, MESH: Ceftazidime, MESH: Drug Resistance, Microbial, Ceftazidime, MESH : SOS Response (Genetics), MESH: beta-Lactamases, MESH : Ceftazidime, medicine.disease_cause, Integron, Integrons, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH : Metronidazole, SOS response, MESH : Anti-Bacterial Agents, Biology (General), MESH: Metronidazole, MESH : Adaptation, Physiological, Genetics, 0303 health sciences, MESH: Microbial Sensitivity Tests, biology, Reverse Transcriptase Polymerase Chain Reaction, MESH: Real-Time Polymerase Chain Reaction, MESH : Genes, Bacterial, MESH : beta-Lactamases, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH: Pseudomonas Infections, Drug Resistance, Microbial, MESH : Adult, MESH : Pseudomonas aeruginosa, MESH : Pseudomonas Infections, Adaptation, Physiological, Anti-Bacterial Agents, Electrophoresis, Gel, Pulsed-Field, Bacterial Pathogens, 3. Good health, Integrase, MESH: Integrons, Infectious Diseases, Gene cassette, MESH: Electrophoresis, Gel, Pulsed-Field, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Pseudomonas aeruginosa, MESH: Pseudomonas aeruginosa, Medicine, MESH: Genes, Bacterial, Research Article, medicine.drug, Adult, MESH : Integrons, Infectious Disease Control, QH301-705.5, Immunology, MESH : Real-Time Polymerase Chain Reaction, Microbial Sensitivity Tests, Real-Time Polymerase Chain Reaction, Microbiology, beta-Lactamases, 03 medical and health sciences, SOS Response (Genetics), Antibiotic resistance, MESH : Drug Resistance, Microbial, Metronidazole, Virology, MESH: Anti-Bacterial Agents, medicine, Humans, MESH: SOS Response (Genetics), Pseudomonas Infections, SOS Response, Genetics, Biology, Molecular Biology, 030304 developmental biology, MESH : Electrophoresis, Gel, Pulsed-Field, Evolutionary Biology, Bacterial Evolution, MESH: Humans, 030306 microbiology, MESH : Humans, Genomic Evolution, Bacteriology, MESH: Adult, RC581-607, biochemical phenomena, metabolism, and nutrition, MESH: Adaptation, Physiological, Genes, Bacterial, biology.protein, bacteria, Parasitology, MESH : Microbial Sensitivity Tests, Immunologic diseases. Allergy
Abstract

Bacterial resistance to β-lactams may rely on acquired β-lactamases encoded by class 1 integron-borne genes. Rearrangement of integron cassette arrays is mediated by the integrase IntI1. It has been previously established that integrase expression can be activated by the SOS response in vitro, leading to speculation that this is an important clinical mechanism of acquiring resistance. Here we report the first in vivo evidence of the impact of SOS response activated by the antibiotic treatment given to a patient and its output in terms of resistance development. We identified a new mechanism of modulation of antibiotic resistance in integrons, based on the insertion of a genetic element, the gcuF1 cassette, upstream of the integron-borne cassette bla OXA-28 encoding an extended spectrum β-lactamase. This insertion creates the fused protein GCUF1-OXA-28 and modulates the transcription, the translation, and the secretion of the β-lactamase in a Pseudomonas aeruginosa isolate (S-Pae) susceptible to the third generation cephalosporin ceftazidime. We found that the metronidazole, not an anti-pseudomonal antibiotic given to the first patient infected with S-Pae, triggered the SOS response that subsequently activated the integrase IntI1 expression. This resulted in the rearrangement of the integron gene cassette array, through excision of the gcuF1 cassette, and the full expression the β-lactamase in an isolate (R-Pae) highly resistant to ceftazidime, which further spread to other patients within our hospital. Our results demonstrate that in human hosts, the antibiotic-induced SOS response in pathogens could play a pivotal role in adaptation process of the bacteria., Author Summary The bacterial SOS response is a conserved regulatory network that is induced in response to DNA damage. Its activation in vitro leads to the emergence of resistance to antibiotics, leading to speculation that this is an important clinical mechanism of acquiring resistance. We found evidence here that antibiotic-induced SOS response plays a role in bacterial genome rearrangement in vivo within humans. The major classes of antibiotics can trigger the bacterial SOS response and our data raise questions about their wide use and their subsequent effect on the bacterial genetic adaptability. This suggests that emergence of antibiotic resistance during therapy could be reduced by inhibiting the bacterial SOS response. We showed that acquired resistance genes could spread latently in susceptible bacterial strains until needed. These findings could impact current policies for control of antibiotic resistance, which rely on the detection of resistant bacteria and on the assumption that resistance mechanisms have a functional cost to the bacteria. More generally, SOS response may spur changes in the behavior of bacteria and their faster adaptation to hostile environments, including humans.

Published
2012
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4. The SOS response controls antibiotic resistance by regulating the integrase of integrons

Author

Marie-Cécile Ploy, Emilie Guerin, Didier Mazel, Guillaume Cambray, Sandra Da Re, Biologie moléculaire et cellulaire des microorganismes (EA3175), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Service de Bactériologie, Virologie, Hygiène [CHU Limoges], CHU Limoges, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biologie moléculaire et cellulaire des microorganismes ( EA3175 ), Université de Limoges ( UNILIM ) -Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Plasticité du Génome Bactérien ( PGB ), and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS )

Subjects
MESH : Drug Resistance, Multiple, Bacterial, MESH : Integrons, [SDV]Life Sciences [q-bio], media_common.quotation_subject, Biodiversité et Ecologie, MESH : Recombination, Genetic, MESH : SOS Response (Genetics), MESH : Promoter Regions, Genetic, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Integron, Antibiotiques, General Biochemistry, Genetics and Molecular Biology, Bactéries multi-résistantes, Biodiversity and Ecology, 03 medical and health sciences, MESH : Integrases, genetique des populations, MESH : Serine Endopeptidases, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, MESH : Bacterial Proteins, MESH : Bacteria, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, media_common, 0303 health sciences, biology, 030306 microbiology, génomique comparative, General Medicine, Art, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, diversité des populations, biology.protein, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Humanities, MESH : Gene Expression Regulation, Bacterial
Abstract

Emilie Guerin, Guillaume Cambray, Sandra Da Re, Didier Mazel, Marie-Cecile Ploy E. Guerin, S. Da Re : Universite de Limoges, EA3175 ; Inserm, equipe Avenir Inserm, faculte de medecine, Limoges, France. G. Cambray, D. Mazel : Institut Pasteur, unite Plasticite du genome bacterien, Paris, 28, rue du Docteur Roux, 75015 Paris, France. M.C. Ploy : Universite de Limoges, EA3175, Equipe Avenir Inserm, Limoges, France. marie-cecile.ploy@unilim.fr NOUVELLE

Published
2010
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5. First occurrence of an IMP metallo-beta-lactamase in Aeromonas caviae: IMP-19 in an isolate from France

Author

Catherine Neuwirth, Frédéric Robin, Richard Bonnet, Eliane Siebor, UFR des Sciences de Santé (Université de Bourgogne), Université de Bourgogne (UB), Télécom ParisTech, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Université d'Auvergne - Clermont-Ferrand I (UdA), Université de Bourgogne ( UB ), Laboratoire Microorganismes : Génome et Environnement ( LMGE ), and Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
Aeromonas caviae, MESH: Sequence Analysis, DNA, MESH : Molecular Sequence Data, MESH: Aeromonas, MESH: beta-Lactamases, Aeromonas punctata, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Integron, Integrons, Substrate Specificity, Plasmid, polycyclic compounds, Pharmacology (medical), MESH : Anti-Bacterial Agents, 0303 health sciences, MESH: Microbial Sensitivity Tests, biology, MESH : Substrate Specificity, MESH: Kinetics, MESH : beta-Lactamases, MESH : Aeromonas, Anti-Bacterial Agents, MESH: Integrons, Infectious Diseases, Aeromonas, France, MESH : Kinetics, Plasmids, MESH : Integrons, Sequence analysis, Molecular Sequence Data, Microbial Sensitivity Tests, beta-Lactams, beta-Lactamases, Microbiology, 03 medical and health sciences, MESH : beta-Lactams, Vibrionaceae, Mechanisms of Resistance, MESH: beta-Lactams, MESH: Plasmids, MESH: Anti-Bacterial Agents, MESH : France, 030304 developmental biology, Pharmacology, MESH: Molecular Sequence Data, 030306 microbiology, Sequence Analysis, DNA, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: France, Kinetics, enzymes and coenzymes (carbohydrates), MESH : Plasmids, biology.protein, bacteria, MESH: Substrate Specificity, MESH : Microbial Sensitivity Tests, Bacteria, MESH : Sequence Analysis, DNA
Abstract

We describe the first IMP metallo-β-lactamase in Aeromonas caviae : IMP-19, which differed from IMP-2 by a single amino acid change (Arg to Ala at position 38). bla IMP-19 was found within a class 1 integron located on a 35-kb plasmid. This is also the first description of an IMP producer in France.

Published
2007
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