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9 results on '"MESH : Metals"'

1. Soil parameters are key factors to predict metal bioavailability to snails based on chemical extractant data

Author

Renaud Scheifler, A. de Vaufleury, Michael Coeurdassier, Benjamin Pauget, Frédéric Gimbert, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)

Subjects
LUMBRICUS-RUBELLUS, MESH: Hydrogen-Ion Concentration, PH, MESH : Biological Availability, Snails, [ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicology, 010501 environmental sciences, MESH: Zinc, 01 natural sciences, MESH : Soil Pollutants, Soil, Soil Pollutants, MESH: Animals, MESH: Snails, MESH : Environmental Monitoring, Assimilation flux, Waste Management and Disposal, MESH : Cadmium, Risk assessment, MESH: Biological Availability, chemistry.chemical_classification, Cadmium, Land snail, Soil chemistry, 04 agricultural and veterinary sciences, Environmental exposure, CONTAMINATED SOILS, MESH: Chemistry Techniques, Analytical, Hydrogen-Ion Concentration, Pollution, 6. Clean water, ORGANIC-MATTER, Zinc, Metals, Environmental chemistry, Aluminum Silicates, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, MESH : Edetic Acid, MESH : Lead, MESH: Environmental Monitoring, MESH: Lead, Environmental Monitoring, MESH : Aluminum Silicates, Environmental Engineering, Soil test, MESH: Edetic Acid, MESH: Environmental Exposure, MESH: Cadmium, Biological Availability, chemistry.chemical_element, HELIX-ASPERSA, Chemistry Techniques, Analytical, MESH: Soil, HEAVY-METALS, MESH : Hydrogen-Ion Concentration, MESH : Soil, Animals, Environmental Chemistry, Organic matter, Chemical method, Edetic Acid, 0105 earth and related environmental sciences, EARTHWORMS, MESH: Soil Pollutants, MESH: Metals, MESH : Metals, MESH : Zinc, Environmental Exposure, MESH : Chemistry Techniques, Analytical, Bioavailability, Transfer, Kinetics, DERMAL UPTAKE, Lead, chemistry, MESH : Snails, 13. Climate action, Soil water, MESH: Aluminum Silicates, 040103 agronomy & agriculture, Clay, 0401 agriculture, forestry, and fisheries, DUTCH FIELD SOILS, MESH : Animals, MESH : Environmental Exposure
Abstract

International audience; Although soil characteristics modulate metal mobility and bioavailability to organisms, they are often ignored in the risk assessment of metal transfer. This paper aims to determine the ability of chemical methods to assess and predict cadmium (Cd), lead (Pb) and zinc (Zn) environmental bioavailability to the land snail Cantareus aspersus. Snails were exposed in the laboratory for 28 days to 17 soils from around a former smelter. The soils were selected for their range of pH, organic matter, clay content, and Cd, Pb and Zn concentrations. The influence of soil properties on environmental availability (estimated using HF-HClO(4), EDTA, CaCl(2), NH(4)NO(3), NaNO(3), free ion activity and total dissolved metal concentration in soil solution) and on environmental bioavailability (modelled using accumulation kinetics) was identified. Among the seven chemical methods, only the EDTA and the total soil concentration can be used to assess Cd and Pb environmental bioavailability to snails (r²(adj)=0.67 and 0.77, respectively). For Zn, none of the chemical methods were suitable. Taking into account the influence of the soil characteristics (pH and CEC) allows a better prediction of Cd and Pb environmental bioavailability (r²(adj)=0.82 and 0.83, respectively). Even though alone none of the chemical methods tested could assess Zn environmental bioavailability to snails, the addition of pH, iron and aluminium oxides allowed the variation of assimilation fluxes to be predicted. A conceptual and practical method to use soil characteristics for risk assessment is proposed based on these results. We conclude that as yet there is no universal chemical method to predict metal environmental bioavailability to snails, and that the soil factors having the greatest impact depend on the metal considered.

Published
2012

2. Dendrochemical patterns of calcium, zinc, and potassium related to internal factors detected by energy dispersive X-ray fluorescence (EDXRF)

Author

Håkan Grudd, Don A. Vroblesky, Michel Chalot, Jean Christophe Balouet, François Beaujard, Kevin T. Smith, Walter C. Shortle, Joel G. Burken, United States Department of Agriculture, International Environmental, Université de Franche-Comté (UFC), Université de Lorraine (UL), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Bolin Centre for Climate Research, Stockholm University, United States Geological Survey [Reston] (USGS), Missouri University of Science and Technology (Missouri S&T), University of Missouri System, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier ( PIAF ), Institut National de la Recherche Agronomique ( INRA ) -Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ), Laboratoire Chrono-environnement (UMR 6249) (LCE), and Université Bourgogne Franche-Comté [COMUE] (UBFC)

Subjects
bois, Wood calcium, Health, Toxicology and Mutagenesis, Potassium, [SDV]Life Sciences [q-bio], Cation distribution, MESH: Zinc, Annual growth %, Trees, MESH : Trees, MESH : Environmental Monitoring, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, MESH: Spectrometry, X-Ray Emission, General Medicine, Contamination, Pollution, Zinc, Metals, Environmental chemistry, MESH: Calcium, Dendrochemistry, Wood potassium, MESH: Environmental Monitoring, Environmental Monitoring, Environmental Engineering, chemistry.chemical_element, X-ray fluorescence, Calcium, dendrochimie, [ SDV.EE ] Life Sciences [q-bio]/Ecology, environment, MESH : Spectrometry, X-Ray Emission, Environmental Chemistry, MESH : Calcium, MESH: Metals, MESH : Metals, Public Health, Environmental and Occupational Health, Spectrometry, X-Ray Emission, General Chemistry, MESH : Zinc, Highly sensitive, MESH: Trees, MESH : Potassium, chemistry, 13. Climate action, MESH: Potassium, Biomineralization
Abstract

International audience; Energy dispersive X-ray fluorescence (EDXRF) provides highly sensitive and precise spatial resolution of cation content in individual annual growth rings in trees. The sensitivity and precision have prompted successful applications to forensic dendrochemistry and the timing of environmental releases of contaminants. These applications have highlighted the need to distinguish dendrochemical effects of internal processes from environmental contamination. Calcium, potassium, and zinc are three marker cations that illustrate the influence of these processes. We found changes in cation chemistry in tree rings potentially due to biomineralization, development of cracks or checks, heartwood/sapwood differentiation, intra-annual processes, and compartmentalization of infection. Distinguishing internal from external processes that affect dendrochemistry will enhance the value of EDXRF for both physiological and forensic investigations.

Published
2014

3. Genome-wide analysis of plant metal transporters, with an emphasis on poplar

Author

Malcolm M. Campbell, Barbara Montanini, Pierre Richaud, Aude Migeon, Damien Blaudez, Olivia Wilkins, Sébastien Thomine, Michel Chalot, Biologie végétale et microbiologie environnementale - UMR7265 (BVME), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Biologie végétale et microbiologie environnementale - UMR7265 ( BVME ), Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Aix Marseille Université ( AMU ), Institut des sciences du végétal ( ISV ), Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Chrono-environnement ( LCE ), and Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC )

Subjects
0106 biological sciences, Populus trichocarpa, MESH: Genome, Plant, [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, MESH : Populus, MESH : Saccharomyces cerevisiae, MESH: Plants, Chlamydomonas reinhardtii, MESH: Carrier Proteins, Saccharomyces cerevisiae, MESH : Plants, Physcomitrella patens, 01 natural sciences, Genome, Fungal Proteins, 03 medical and health sciences, Cellular and Molecular Neuroscience, Selaginella moellendorffii, Botany, Arabidopsis thaliana, Gene family, MESH : Fungal Proteins, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, MESH: Phylogeny, Molecular Biology, Gene, Phylogeny, 030304 developmental biology, Pharmacology, 0303 health sciences, MESH: Metals, biology, MESH : Metals, MESH : Phylogeny, food and beverages, Cell Biology, Plants, biology.organism_classification, MESH: Saccharomyces cerevisiae, MESH : Genome, Plant, MESH: Populus, Populus, Metals, Molecular Medicine, MESH: Fungal Proteins, MESH : Carrier Proteins, Carrier Proteins, Genome, Plant, 010606 plant biology & botany
Abstract

International audience; The specific transport of metal ions, mediated by membrane-localized metal transporters, is of fundamental importance in all eukaryotes. Genome-wide analysis of metal transporters was undertaken, making use of whole genome sequences of the green alga Chlamydomonas reinhardtii, the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii, the monocots rice and sorghum, and the dicots Arabidopsis thaliana, poplar, grapevine, as well as of the yeast Saccharomyces cerevisiae. A repertoire of 430 metal transporters was found in total across eight photosynthetic plants, as well as in S. cerevisiae. Seventy-two full-length metal transporter genes were identified in the Populus genome alone, which is the largest number of metal transporters genes identified in any single species to date. Diversification of some transporter family gene clusters appears to have occurred in a lineage-specific manner. Expression analysis of Populus metal transporters indicates that some family members show tissue-specific transcript abundance. Taken together, the data provide a picture into the diversification of these important gene families.

Published
2010

4. Functional characterization of NRAMP3 and NRAMP4 from the metal hyperaccumulator Thlaspi caerulescens

Author

Pierre Richaud, Ronald Oomen, Hélène Barbier-Brygoo, Jian Wu, Sébastien Thomine, Françoise Lelièvre, Mark G. M. Aarts, Sandrine Blanchet, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratory of Genetics, Wageningen University and Research [Wageningen] (WUR), Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS), Bioénergie et Microalgues (EBM), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Biologie végétale et microbiologie environnementale - UMR7265 (BVME), UMR Biolologie Végétale & Microbiologie Environementale, Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research Centre [Wageningen] (WUR), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut des sciences du végétal ( ISV ), Centre National de la Recherche Scientifique ( CNRS ), Biochimie et Physiologie Moléculaire des Plantes ( BPMP ), Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Wageningen University and Research Centre [Wageningen] ( WUR ), Institute of Vegetables and Flowers ( IVF ), Chinese Academy of Agricultural Sciences ( CAAS ), Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues ( L3BM ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Biologie végétale et microbiologie environnementale - UMR7265 ( BVME ), and Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Aix Marseille Université ( AMU )

Subjects
0106 biological sciences, iron transport, Mutant, Arabidopsis, Plant Science, MESH : Green Fluorescent Proteins, 01 natural sciences, Laboratorium voor Plantenveredeling, Arabidopsis thaliana, MESH : Cadmium, transporter genes, 0303 health sciences, food and beverages, NRAMP, Zinc, Laboratory of Genetics, MESH : Vacuoles, MESH : Cloning, Molecular, zinc transporter, Recombinant Fusion Proteins, Molecular Sequence Data, Saccharomyces cerevisiae, Laboratorium voor Erfelijkheidsleer, MESH: Vacuoles, 03 medical and health sciences, MESH: Green Fluorescent Proteins, Thlaspi caerulescens, Botany, MESH: Recombinant Fusion Proteins, MESH: Cloning, Molecular, MESH: DNA, Plant, MESH: Metals, MESH: Molecular Sequence Data, MESH : Metals, fungi, Plant Breeding, Mutation, MESH : Gene Expression Regulation, Plant, MESH: Genome, Plant, [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, MESH : Molecular Sequence Data, MESH : DNA, Complementary, Physiology, MESH : Saccharomyces cerevisiae, MESH : Thlaspi, medicine.disease_cause, MESH: Zinc, expression differences, MESH : Arabidopsis, Gene Expression Regulation, Plant, MESH: Arabidopsis, Cloning, Molecular, Plant Proteins, metal transport, zinc (Zn), MESH: Genetic Complementation Test, biology, MESH: Plant Proteins, EPS-3, MESH: Saccharomyces cerevisiae, MESH : Genome, Plant, MESH: Intracellular Membranes, MESH : DNA, Plant, Biochemistry, Metals, saccharomyces-cerevisiae, MESH : Mutation, hyperaccumulator, Genome, Plant, DNA, Complementary, MESH: Mutation, MESH : Recombinant Fusion Proteins, DNA, Plant, cadmium, heavy-metal, MESH: Biological Transport, Green Fluorescent Proteins, MESH: Cadmium, MESH: Thlaspi, arabidopsis-thaliana, MESH : Plant Proteins, medicine, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Hyperaccumulator, MESH: Gene Expression Regulation, Plant, 030304 developmental biology, MESH : Genetic Complementation Test, cadmium (Cd), ACL, Genetic Complementation Test, Biological Transport, MESH : Zinc, Intracellular Membranes, MESH: DNA, Complementary, biology.organism_classification, Yeast, Thlaspi, MESH : Biological Transport, MESH : Intracellular Membranes, Vacuoles, Zinc toxicity, gene encodes, Heterologous expression, protein, 010606 plant biology & botany
Abstract

International audience; The ability of metal hyperaccumulating plants to tolerate and accumulate heavy metals results from adaptations of metal homeostasis. NRAMP metal transporters were found to be highly expressed in some hyperaccumulating plant species. Here, we identified TcNRAMP3 and TcNRAMP4, the closest homologues to AtNRAMP3 and AtNRAMP4 in Thlaspi caerulescens and characterized them by expression analysis, confocal imaging and heterologous expression in yeast and Arabidopsis thaliana. TcNRAMP3 and TcNRAMP4 are expressed at higher levels than their A. thaliana homologues. When expressed in yeast TcNRAMP3 and TcNRAMP4 transport the same metals as their respective A. thaliana orthologues: iron (Fe), manganese (Mn) and cadmium (Cd) but not zinc (Zn) for NRAMP3; Fe, Mn, Cd and Zn for NRAMP4. They also localize at the vacuolar membrane in A. thaliana protoplasts. Inactivation of AtNRAMP3 and AtNRAMP4 in A. thaliana results in strong Cd and Zn hypersensitivity, which is fully rescued by TcNRAMP3 or TcNRAMP4 expression. However, metal tolerance conferred by TcNRAMP expression in nramp3nramp4 mutant does not exceed that of wild-type A. thaliana. Our data indicate that the difference between TcNRAMP3 and TcNRAMP4 and their A. thaliana orthologues does not lie in a different protein function, but probably resides in a different expression level or expression pattern.

Published
2009

5. A tale of two oxidation states: bacterial colonization of arsenic-rich environments

Author

Caroline Dossat, Barbara Schoepp, Jean Weissenbach, Yuko Makita, Valérie Barbe, Patricia Siguier, Béatrice Segurens, Michael Chandler, Wolfgang Nitschke, Sophie Mangenot, Zoé Rouy, Stéphanie Weiss, David Vallenet, Benoit Cournoyer, Diliana D. Simeonova, Philippe Ortet, Antoine Danchin, Florence Arsène-Ploetze, Sandrine Koechler, Christine Carapito, Mohamed Barakat, Evelyne Krin, Didier Lièvremont, Daniel Muller, Emmanuelle Leize, Simon Duval, Philippe N. Bertin, Marie-Claire Lett, Nicolas Perdrial, Michaël Heymann, Stéphane Cruveiller, Aurélie Lieutaud, Violaine Bonnefoy, Evelyne Turlin, Claudine Médigue, Emmanuel Talla, Alain Van Dorsselaer, Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'Ecologie Microbienne de la Rhizosphère et d'Environnements Extrêmes (LEMIRE), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Génétique des Génomes Bactériens, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pluridisciplinaire Hubert Curien (IPHC), Laboratoire de microbiologie et génétique moléculaires (LMGM), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre de géochimie de la surface (CGS), Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut national des sciences de l'Univers (INSU - CNRS), Structure et évolution des génomes (SEG), CNS-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de microbiologie et génétique moléculaires - UMR5100 (LMGM), Centre de Biologie Intégrative (CBI), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Génétique moléculaire, génomique, microbiologie ( GMGM ), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique ( CNRS ), Genoscope - Centre national de séquençage [Evry] ( GENOSCOPE ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Laboratoire d'Ecologie Microbienne de la Rhizosphère et d'Environnements Extrêmes ( LEMIRE ), Université de la Méditerranée - Aix-Marseille 2-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de chimie bactérienne ( LCB ), Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut Pluridisciplinaire Hubert Curien ( IPHC ), Laboratoire de microbiologie et génétique moléculaires ( LMGM ), Université Paul Sabatier - Toulouse 3 ( UPS ) -Centre National de la Recherche Scientifique ( CNRS ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Centre de géochimie de la surface ( CGS ), Institut national des sciences de l'Univers ( INSU - CNRS ) -Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique ( CNRS ), Structure et évolution des génomes ( SEG ), and CNS-Université d'Évry-Val-d'Essonne ( UEVE ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
MESH: Oxidation-Reduction, Cancer Research, Drug Resistance, MESH : Models, Biological, QH426-470, MESH : Carbon, MESH : Drug Resistance, Bacterial, MESH: Genome, Bacterial, Arsenic, Bacteria, Biodegradation, Environmental, Carbon, Bacterial, Energy Metabolism, Genome, Metals, Models, Biological, Oxidation-Reduction, Phylogeny, MESH: Biodegradation, Environmental, 0302 clinical medicine, Herminiimonas arsenicoxydans, MESH: Phylogeny, ComputingMilieux_MISCELLANEOUS, Genetics (clinical), 0303 health sciences, Ecology, MESH: Energy Metabolism, MESH : Arsenic, 6. Clean water, Biodegradation, Environmental, Research Article, inorganic chemicals, MESH : Biodegradation, Environmental, MESH : Genome, Bacterial, Energy metabolism, chemistry.chemical_element, MESH: Carbon, Biology, Genomic databases, Microbiology, Models, Biological, 03 medical and health sciences, Bacterial colonization, Bioremediation, Drug Resistance, Bacterial, MESH: Arsenic, MESH: Drug Resistance, Bacterial, SDV:BBM, Genetics, MESH : Bacteria, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, MESH : Oxidation-Reduction, MESH: Metals, 030306 microbiology, MESH : Metals, MESH: Models, Biological, MESH : Phylogeny, Computational Biology, Oxidation reduction, Genetics and Genomics, biology.organism_classification, Archaea, MESH : Energy Metabolism, Eubacteria, MESH: Bacteria, Positive chemotaxis, chemistry, 13. Climate action, Genome, Bacterial, 030217 neurology & neurosurgery
Abstract

Microbial biotransformations have a major impact on contamination by toxic elements, which threatens public health in developing and industrial countries. Finding a means of preserving natural environments—including ground and surface waters—from arsenic constitutes a major challenge facing modern society. Although this metalloid is ubiquitous on Earth, thus far no bacterium thriving in arsenic-contaminated environments has been fully characterized. In-depth exploration of the genome of the β-proteobacterium Herminiimonas arsenicoxydans with regard to physiology, genetics, and proteomics, revealed that it possesses heretofore unsuspected mechanisms for coping with arsenic. Aside from multiple biochemical processes such as arsenic oxidation, reduction, and efflux, H. arsenicoxydans also exhibits positive chemotaxis and motility towards arsenic and metalloid scavenging by exopolysaccharides. These observations demonstrate the existence of a novel strategy to efficiently colonize arsenic-rich environments, which extends beyond oxidoreduction reactions. Such a microbial mechanism of detoxification, which is possibly exploitable for bioremediation applications of contaminated sites, may have played a crucial role in the occupation of ancient ecological niches on earth., Author Summary Microorganisms play a crucial role in nutrient biogeochemical cycles. Arsenic is found throughout the environment from both natural and anthropogenic sources. Its inorganic forms are highly toxic and impair the physiology of most higher organisms. Arsenic contamination of groundwater supplies is giving rise to increasingly severe human health problems in both developing and industrial countries. In the present work, we investigated the metabolism of this metalloid in Herminiimonas arsenicoxydans, a representative organism of a novel bacterial genus widespread in aquatic environments. Examination of the genome sequence and experimental evidence revealed that it is remarkably capable of coping with arsenic. Our observations support the existence of multiple strategies allowing arsenic-metabolizing microbes to efficiently colonize toxic environments. In particular, arsenic oxidation and scavenging may have played a crucial role in the development of early stages of life on Earth. Such mechanisms may one day be exploited as part of a potential bioremediation strategy in toxic environments.

Published
2007

6. Identification of genes and proteins involved in the pleiotropic response to arsenic stress in Caenibacter arsenoxydans, a metalloresistant beta-proteobacterium with an unsequenced genome

Author

Emmanuelle Leize-Wagner, Sandrine Koechler, Evelyne Turlin, Christine Carapito, Philippe N. Bertin, Alain Van Dorsselaer, Antoine Danchin, Marie-Claire Lett, Daniel Muller, Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Laboratoire de Biologie Tumorale, CRLCC Paul Strauss, XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Génétique des Génomes Bactériens, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Génétique moléculaire, génomique, microbiologie (GMGM), Régulation de l'Expression Génétique (REG), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Compartimentation et dynamique cellulaires (CDC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Substances naturelles/chimie moléculaire, Université Louis Pasteur - Strasbourg I-Ecole européenne de chimie, polymères et matériaux [Strasbourg]-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Spectrométrie de masse Bio-organique, UMR CNRS-ULP, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Pluridisciplinaire Hubert Curien ( IPHC ), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique ( CNRS ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), XLIM ( XLIM ), Université de Limoges ( UNILIM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Génétique moléculaire, génomique, microbiologie ( GMGM ), Régulation de l'Expression Génétique ( REG ), Sciences Analytiques et Interactions Ioniques et Biomoléculaires ( DSA-IPHC ), Compartimentation et dynamique cellulaires ( CDC ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -INSTITUT CURIE-Centre National de la Recherche Scientifique ( CNRS ), Université Louis Pasteur - Strasbourg I-Ecole européenne de chimie, polymères et matériaux [Strasbourg]-Centre National de la Recherche Scientifique ( CNRS ), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH : Molecular Sequence Data, MESH : RNA, Messenger, Messenger, Drug Resistance, MESH: Amino Acid Sequence, MESH : Drug Resistance, Bacterial, MESH: Genome, Bacterial, Biochemistry, Genome, chemistry.chemical_compound, MESH : Bacterial Proteins, MESH: Betaproteobacteria, Peptide sequence, MESH: Bacterial Proteins, MESH : Betaproteobacteria, Arsenite, Genetics, 0303 health sciences, MESH : Amino Acid Sequence, MESH : Genes, Bacterial, Bacterial, Betaproteobacteria, General Medicine, MESH : Arsenic, Metals, MESH: Genes, Bacterial, MESH : Genome, Bacterial, Molecular Sequence Data, chemistry.chemical_element, Biology, Arsenic, 03 medical and health sciences, Bacterial Proteins, Drug Resistance, Bacterial, MESH: Arsenic, MESH: Drug Resistance, Bacterial, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, RNA, Messenger, Amino Acid Sequence, Gene, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030304 developmental biology, MESH: RNA, Messenger, Two-dimensional gel electrophoresis, MESH: Metals, MESH: Molecular Sequence Data, 030306 microbiology, MESH : Metals, Arsenate, RNA, chemistry, Genes, Genes, Bacterial, Genome, Bacterial
Abstract

International audience; The effect of high concentrations of arsenic has been investigated in Caenibacter arsenoxydans, a beta-proteobacterium isolated from an arsenic contaminated environment and able to oxidize arsenite to arsenate. As the genome of this bacterium has not yet been sequenced, the use of a specific proteomic approach based on nano-high performance liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) studies and de novo sequencing to perform cross-species protein identifications was necessary. In addition, a random mutational analysis was performed. Twenty-two proteins and 16 genes were shown to be differentially accumulated and expressed, respectively, in cells grown in the presence of arsenite. Two genes involved in arsenite oxidation and one in arsenite efflux as well as two proteins responsible for arsenate reduction were identified. Moreover, numerous genes and proteins belonging to various functional classes including information and regulation pathways, intermediary metabolism, cell envelope and cellular processes were also up- or down-regulated, which demonstrates that bacterial response to arsenic is pleiotropic.

Published
2006

7. How terrestrial snails can be used in risk assessment of soils

Author

Pascal Pandard, Renaud Scheifler, Christiane Lovy, Nadia Crini, Pierre-Marie Badot, Michaël Cœurdassier, Annette de Vaufleury, Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Unité sous contrat biologie environnementale, Institut National de la Recherche Agronomique (INRA)-Université de Franche-Comté (UFC), Institut National de l'Environnement Industriel et des Risques (INERIS), Agence de l'Environnement et de la Maîtrise de l'Energie (ADEME, contract n° 01 75 037), the Ministère de l'Education Nationale de la Recherche et de la Technologie (contract n°98C 0266), Conseil régional de Franche-Comte, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Institut National de la Recherche Agronomique ( INRA ) -Université de Franche-Comté ( UFC ), and Institut National de l'Environnement Industriel et des Risques ( INERIS )

Subjects
MESH : Biological Availability, [SDV]Life Sciences [q-bio], Health, Toxicology and Mutagenesis, Snails, 0211 other engineering and technologies, [ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicology, Sewage, MESH : Dose-Response Relationship, Drug, 02 engineering and technology, Snail, 010501 environmental sciences, MESH: Risk Assessment, 01 natural sciences, MESH : Toxicity Tests, MESH: Dose-Response Relationship, Drug, MESH : Soil Pollutants, Soil, MESH : Ecosystem, Gastropoda, Soil Pollutants, MESH: Animals, MESH: Ecosystem, MESH : Environmental Monitoring, MESH : Risk Assessment, MESH: Snails, MESH : Cadmium, MESH: Biological Availability, biology, Ecology, Heavy, MESH: Metals, Heavy, Soil contamination, Metals, HELIX ASPERSA, Environmental chemistry, MESH : Metals, Heavy, Biological Assay, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Drug, MESH: Environmental Monitoring, Cadmium, Environmental Monitoring, MESH: Cadmium, Biological Availability, MESH: Biological Assay, Risk Assessment, Dose-Response Relationship, MESH: Soil, Metals, Heavy, MESH : Soil, biology.animal, Toxicity Tests, Environmental Chemistry, Ecotoxicology, Animals, MESH: Toxicity Tests, Ecosystem, MESH: Sewage, 0105 earth and related environmental sciences, Invertebrate, 021110 strategic, defence & security studies, MESH: Soil Pollutants, MESH: Metals, Dose-Response Relationship, Drug, business.industry, MESH : Metals, biology.organism_classification, MESH : Sewage, MESH : Snails, 13. Climate action, MESH : Animals, MESH : Biological Assay, Ecotoxicity, business, Sludge
Abstract

International audience; Among soil invertebrates, terrestrial snails are herbivorous and detritivorous organisms exposed to polluted soils by both digestive and cutaneous routes. Using laboratory-reared snails (Helix aspersa aspersa), we describe how the effects of contaminants on survival and growth of snails can be evaluated in laboratory bioassays. A national ring test was performed to assess the effect of Cd added to the soil or to the food. The ecotoxicity of sewage sludge also was evaluated. The present results demonstrate that toxicity depends on both the pollutants and the exposure route. Cadmium was sixfold more toxic for snails exposed via food contamination (median effective concentration [EC50], 68-139 microg/g) than via soil contamination (EC50, 534-877 microg/g), whereas the opposite occurred with the sewage sludge (EC50, 55% of sludge in the food and 10% of waste in the soil). A logistic relationship linked growth inhibition and internal Cd concentrations, which can reach 2,000 microg/g in the viscera of snails exposed to 626 microg/g in the food. No clear trend was found between Cu, Zn, Pb, Cr, and Ni concentrations in the sludge and in snail tissues. These data enabled the development of an international standard, which should enhance the use of terrestrial gastropods for both fundamental research and routine risk assessment in the terrestrial environment.

Published
2006

8. Transfer of Cd, Cu, Ni, Pb, and Zn in a soil-plant-invertebrate food chain: a microcosm study

Author

Michaël Cœurdassier, Annette de Vaufleury, Nadia Crini, Renaud Scheifler, Pierre-Marie Badot, Unité sous contrat biologie environnementale, Institut National de la Recherche Agronomique (INRA)-Université de Franche-Comté (UFC), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), ADEME (Agence de l'Environnement et de la Maîtrise de l'Energie, project 98 93 021) and by the Conseil Régional de Franche-Comte, Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), ProdInra, Migration, Institut National de la Recherche Agronomique ( INRA ) -Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement ( LCE ), and Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC )

Subjects
EVALUATION DU RISQUE, Time Factors, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], [ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicology, Lactuca, Snail, MESH: Risk Assessment, Helix (Snails), MESH : Soil Pollutants, Soil, MESH : Ecosystem, MESH: Helix (Snails), Soil Pollutants, MESH: Animals, MESH: Ecosystem, MESH : Environmental Monitoring, MESH : Risk Assessment, Cadmium, Ecology, Lettuce, MESH : Food Chain, [SDV] Life Sciences [q-bio], Metals, Bioaccumulation, Environmental chemistry, MESH : Helix (Snails), Biological Assay, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Microcosm, MESH: Environmental Monitoring, MESH : Time Factors, Environmental Monitoring, MESH: Ecology, SOL POLLUE, Food Chain, Soil test, chemistry.chemical_element, ESCARGOT PIETIT-GRIS, Biology, MESH: Lettuce, MESH: Biological Assay, Risk Assessment, MESH: Soil, biology.animal, MESH : Soil, Botany, parasitic diseases, Environmental Chemistry, Ecotoxicology, Animals, MESH: Food Chain, Ecosystem, MESH: Soil Pollutants, MESH: Metals, Helix, Snails, MESH : Metals, fungi, MESH: Time Factors, MESH : Lettuce, biology.organism_classification, MESH : Ecology, chemistry, Soil water, MESH : Animals, MESH : Biological Assay
Abstract

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; The transfer of Cd, Cu, Ni, Ph, and Zn was evaluated in a soil-plant (lettuce, Lactuca sativa)-invertebrate (snail, Helix aspersa) food chain during a microcosm experiment. Two agricultural soils, polluted and unpolluted, were studied. Lettuce was cultivated for eight weeks before introduction of snails into the microcosms (M-snails). In a parallel experiment, snails were exposed to lettuce only (i.e., without soil) in simpler exposure devices called containers (C-snails). Snail exposure duration was eight weeks for both M- and C-snails. No effects on snail survival were found. Both M- and C-snails exposed to polluted soil showed a growth reduction, but only after two weeks of exposure. Time-dependent accumulation in M-snails exposed to the polluted environment showed a regular increase of Cd and Zn concentrations over time and a rapid increase of Ph concentrations within the first two weeks, which then remained stable. Copper and Ni concentrations did not increase during any of the experiments. Concentrations in M- and C-snails were compared to estimate the relative contribution of soil and plant to the total bioaccumulation. The results suggest that the soil contribution may be higher than 80% for Pb, from 30 to 60% for Zn, and from 2 to 40% for Cd

Published
2006

9. A field method using microcosms to evaluate transfer of Cd, Cu, Ni, Pb and Zn from sewage sludge amended forest soils to Helix aspersa snails

Author

A. Gomot-de Vaufleury, Pierre-Marie Badot, Jean-Michel Carnus, M Ben Brahim, Renaud Scheifler, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Centre de Sfax, Institut National de la Recherche Agronomique ( INRA ), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut National de la Recherche Agronomique (INRA), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)

Subjects
MESH : Biological Availability, Health, Toxicology and Mutagenesis, MESH: Forestry, [ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicology, 010501 environmental sciences, Toxicology, 01 natural sciences, MESH: Zinc, MESH : Soil Pollutants, Food chain, Nickel, MESH: Helix (Snails), Biomonitoring, MESH: Nickel, Soil Pollutants, MESH: Animals, MESH : Environmental Monitoring, MESH : Copper, MESH : Cadmium, MESH: Biological Availability, Sewage, biology, Chemistry, Helix (gastropod), Forestry, 04 agricultural and veterinary sciences, General Medicine, Pollution, 6. Clean water, MESH: Copper, Zinc, Metals, Environmental chemistry, MESH : Helix (Snails), [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, MESH : Lead, Microcosm, MESH: Environmental Monitoring, MESH: Lead, Cadmium, Environmental Monitoring, MESH: Cadmium, Biological Availability, Animals, MESH: Sewage, 0105 earth and related environmental sciences, Invertebrate, MESH: Soil Pollutants, MESH: Metals, Helix, Snails, MESH : Metals, MESH : Zinc, biology.organism_classification, Bioavailability, Lead, MESH : Sewage, Soil water, 040103 agronomy & agriculture, MESH : Forestry, 0401 agriculture, forestry, and fisheries, MESH : Animals, MESH : Nickel, Copper, Sludge
Abstract

International audience; Juvenile Helix aspersa snails exposed in field microcosms were used to assess the transfer of Cd, Cu, Ni, Pb and Zn from forest soils amended with liquid and composted sewage sludge. Zn concentrations and contents were significantly higher in snails exposed to liquid and composted sludge after 5 and 7 weeks of exposure, when compared with control. Trends were less clear for the other metals. Present results show that Zn, among the cocktail of metallic trace elements (MTE) coming from sewage sludge disposal, represents the principal concern for food chain transfer and secondary poisoning risks. The microcosm design used in this experiment was well suited for relatively long-term (about 2 months) active biomonitoring with H. aspersa snails. The snails quickly indicated the variations of MTE concentrations in their immediate environment. Therefore, the present study provides a simple but efficient field tool to evaluate MTE bioavailability and transfer.

Published
2002

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