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17 results on '"MESH : Oxidative Stress"'

1. CD36 gene polymorphism is associated with Alzheimer's disease

Author

Vladimir J. Balcar, Alice Hálová, Vladimir Janout, Laura Ewerlingová, Jan Lochman, Jana Janoutová, Naim Akhtar Khan, Omar Šerý, Department of biochemistry, Masaryk University, Masaryk University [Brno] (MUNI), Institute of Animal Physiology and Genetics (Brno), Institute of Animal Physiology and Genetics, Department of Epidemiology and Public Health [university of Ostrava], Lékařská fakulta / Faculty of Medicine [University of Ostrava], Ostravská univerzita / University of Ostrava-Ostravská univerzita / University of Ostrava, Equipe NuTox (LNC - U1231) (NUTOX), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Bosch Institute [Sydney], The University of Sydney, Discipline of Anatomy and Histology {University of Sydney], Masaryk University, Equipe NuTox (LNC - U1231) ( NUTOX ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ), and The University of Sydney [Sydney]

Subjects
0301 basic medicine, Apolipoprotein E, MESH : Oxidative Stress, CD36 Antigens, Male, MESH : Polymorphism, Genetic, CD36, MESH : Aged, MESH : Alzheimer Disease, MESH : Genotype, Biochemistry, Gene, MESH: Genotype, 0302 clinical medicine, MESH: Cholesterol, MESH : Female, MESH : Cholesterol, Genetics, MESH: Aged, MESH: Oxidative Stress, biology, MESH: Polymorphism, Single Nucleotide, MESH : Polymorphism, Single Nucleotide, MESH: Genetic Predisposition to Disease, General Medicine, 3. Good health, MESH : Antigens, CD36, Cholesterol, Interleukin 18, Female, ApoE, Genotype, MESH : Male, Single-nucleotide polymorphism, Polymorphism, Single Nucleotide, MMSE, Association, 03 medical and health sciences, Alzheimer Disease, MESH: Polymorphism, Genetic, SNP, Humans, Genetic Predisposition to Disease, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Allele, Polymorphism, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Genetic association, Aged, Polymorphism, Genetic, MESH: Humans, MESH: Antigens, CD36, MESH : Humans, MESH: Male, Oxidative Stress, 030104 developmental biology, biology.protein, MESH : Genetic Predisposition to Disease, Gene polymorphism, MESH: Female, 030217 neurology & neurosurgery, MESH: Alzheimer Disease
Abstract

IF 3.112; International audience; CD36 gene encodes a membrane glycoprotein (type B scavenger receptor) present on the surface of many types of cells and having multiple cellular functions ranging from angiogenesis to gustatory perception of fatty acids. Using a case control genetic association approach we have analyzed selected single nucleotide polymorphisms (SNP's) in a total of 859 patients with Alzheimer's disease (AD) and controls and have identified the allele A in rs3211892 polymorphism of CD36 gene as significantly increasing the risk of AD. Additionally we have investigated, in the same sample of control subjects and patients, SNP's in ApoE gene and confirmed that the previously identified AD-associated SNP's indeed increased the risk and decreased the age of onset of AD as reported by others earlier. Based on the current knowledge of CD36 biochemistry we propose that the AD risk-imparting variants of CD36 alter cholesterol homeostasis, oxidation stress or induce pathological inflammatory cascades. The SNP rs3211892 has previously been associated with heart disease and other conditions but the present study is the first to identify a significant association between variations in CD36 gene and the risk of Alzheimer's disease.

Published
2017
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2. Chitosan nanoparticles plus quercetin suppress the oxidative stress, modulate DNA fragmentation and gene expression in the kidney of rats fed ochratoxin A-contaminated diet

Author

Abdulhadi Aljawish, Mosaad A. Abdel-Wahhab, Nabila S. Hassan, Sekena H. Abdel-Aziem, Aziza A. El-Nekeety, National Research Center ( NRC ), National Research Center, Equipe NuTox (LNC - U1231) ( NUTOX ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM )

Subjects
0301 basic medicine, Ochratoxin A, Male, MESH : Oxidative Stress, MESH : Carcinogens, Antioxidant, medicine.medical_treatment, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, Pharmacology, Toxicology, medicine.disease_cause, Kidney, Antioxidants, Rats, Sprague-Dawley, chemistry.chemical_compound, 0302 clinical medicine, Malondialdehyde, MESH : Ochratoxins, MESH : Protective Agents, MESH : DNA Fragmentation, Nephrotoxicity, Chemistry, MESH : Rats, General Medicine, Ochratoxins, MESH : Kidney, Drug Combinations, medicine.anatomical_structure, MESH : Drug Combinations, Biochemistry, Liver, 030220 oncology & carcinogenesis, MESH : Quercetin, DNA fragmentation, MESH : Antioxidants, Quercetin, MESH : Chitosan, MESH : Male, Food Contamination, DNA Fragmentation, Protective Agents, MESH : Diet, 03 medical and health sciences, medicine, Animals, MESH : Malondialdehyde, Chitosan, technology, industry, and agriculture, MESH : Liver, Mycotoxins, Chitosan nanoparticles, MESH : Rats, Sprague-Dawley, Diet, Rats, MESH : Food Contamination, 030104 developmental biology, Oxidative stress, MESH : Nanoparticles, Carcinogens, Nanoparticles, MESH : Animals, Genotoxicity, Food Science
Abstract

IF 3.778; International audience; This study aimed to evaluate the protective role of chitosan nanoparticles (COS-NPs) singly or plus quercetin (Q) against OTA-induced oxidative stress and renal genotoxicity. Twelve groups of male Sprague-Dawley rats were treated orally for 3 weeks included the control group, animals fed OTA-contaminated diet (3 mg/kg diet); COS-NPs-treated groups at low (140 mg/kg b.w.) or high (280 mg/kg b.w.) dose, Q-treated group (50 mg/kg b.w.), Q plus low or high dose of COS-NPs-treated groups and OTA plus Q and/or COS-NPs at the two tested doses-treated groups. The results indicated that COS-NPs were roughly rod in shape with average particle size of 200 nm and zeta potential 31.4 ± 2.8 mV. Animals fed OTA-contaminated diet showed significant changes in serum biochemical parameters, increase kidney MDA and DNA fragmentation and decreased GPx and SOD gene expression accompanied with severe histological changes. Q and/or COS-NPs at the two tested doses induced significant improvements in all tested parameters and succeeded to overcome these effects especially in the animals treated with Q plus the high dose of COS-NPs. It could be concluded that COS-NPs are promise candidate to enhance the antioxidant effect of Q and protect against the nephrotoxicity of OTA in high endemic areas.

Published
2017
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3. Grape seed and skin extract reduces pancreas lipotoxicity, oxidative stress and inflammation in high fat diet fed rats

Author

Naim Akhtar Khan, Kamel Charradi, Ezzedine Aouani, Faten Aloui, Selvakumar Subramaniam, Aziz Hichami, Ferid Limam, Faculté des Sciences de Bizerte [Université de Carthage], Université de Carthage - University of Carthage, Laboratoire des Substances Bioactives (Centre de Biotechnologie de Borj Cedria, Hammam-Lif) (LSBA), Centre de Biotechnologie de Borj Cédria (Hammam-Lif, Tunisie), Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7) (NUTox), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Faculté des Sciences de Bizerte, Laboratoire des Substances Bioactives (Centre de Biotechnologie de Borj Cedria, Hammam-Lif) ( LSBA ), Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7) ( NUTox ), Lipides - Nutrition - Cancer (U866) ( LNC ), and Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA )

Subjects
Male, MESH : Oxidative Stress, 0301 basic medicine, MESH: Inflammation, Antioxidant, medicine.medical_treatment, MESH: Grape Seed Extract, medicine.disease_cause, Antioxidants, chemistry.chemical_compound, 0302 clinical medicine, MESH: Obesity, Vitis, MESH: Animals, MESH : Pancreas, 2. Zero hunger, MESH: Oxidative Stress, MESH : Grape Seed Extract, MESH : Rats, MESH : Diet, High-Fat, food and beverages, General Medicine, MESH: Pancreas, 3. Good health, Lipotoxicity, 030220 oncology & carcinogenesis, Seeds, MESH : Antioxidants, MESH : Obesity, medicine.symptom, medicine.medical_specialty, Grape seed and skin extract, MESH: Rats, MESH : Male, Inflammation, Biology, Diet, High-Fat, MESH : Rats, Wistar, MESH: Vitis, MESH : Vitis, 03 medical and health sciences, Diabetes mellitus, Internal medicine, medicine, Animals, Obesity, Rats, Wistar, Pancreas, Pharmacology, MESH : Inflammation, Grape Seed Extract, Triglyceride, Cholesterol, MESH: Antioxidants, [ SDV.SP.PHARMA ] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, MESH: Rats, Wistar, medicine.disease, MESH : Seeds, MESH: Male, Rats, MESH: Diet, High-Fat, 030104 developmental biology, Endocrinology, chemistry, MESH: Seeds, Oxidative stress, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, MESH : Animals, Steatosis
Abstract

IF 2.326; International audience; Obesity is related to an elevated risk of diabetes and the mechanisms whereby fat adversely affects the pancreas are poorly understood. We studied the effect of a high fat diet (HFD) on pancreas steatosis, oxidative stress and inflammation as well as the putative protection afforded by grape seed and skin extract (GSSE). HFD induced body weight gain, without affecting insulinemia, nor glycemia and dropped adiponectemia. HFD also provoked the ectopic deposition of cholesterol and triglyceride, and an oxidative stress characterized by increased lipoperoxidation and carbonylation, inhibition of antioxidant enzyme activities such as CAT, GPx and SOD, depletion of zinc and a concomitant increase in calcium and H2O2. HFD induced pro-inflammatory chemokines mRNA as RANTES and MCP1 as well as cytokines expression as TNFα, IL6 and IL1β. Importantly GSSE counteracted all the deleterious effects of HFD on pancreas in vivo i-e lipotoxicity, oxidative stress and inflammation. In conclusion, GSSE could find potential applications in fat-induced pancreas lipotoxicity and dysfunction.

Published
2016
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4. Functional and structural insights of a Staphylococcus aureus apoptotic-like membrane peptide from a toxin-antitoxin module

Author

Nour Sayed, Stéphane Réty, Sylvie Nonin-Lecomte, Brice Felden, Fonction, structure et inactivation d'ARN bactériens, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire de cristallographie et RMN biologiques (LCRB - UMR 8015), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Inserm, CNRS, Région Bretagne (Ph.D grant for N.S.), FRM (grant to N.S.) Ministère de l'enseignement supérieur et de la recherche, Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Etude du 'Rnome' bacterien. Identification et Caracterisation d'Acides Ribonucleiques Impliques Dans la Survie et la Virulence', Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Lefevre, Annick, Nonin-Lecomte, Sylvie, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire de cristallographie et RMN biologiques ( LCRB - UMR 8015 ), and Université Paris Descartes - Paris 5 ( UPD5 ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
MESH : Oxidative Stress, MESH: Protein Structure, Secondary, medicine.disease_cause, Biochemistry, Protein Structure, Secondary, MESH: Circular Dichroism, Cell membrane, chemistry.chemical_compound, MESH: Nuclear Magnetic Resonance, Biomolecular, MESH: Staphylococcus aureus, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, MESH: Oxidative Stress, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MESH : Peptides, MESH: Peptides, MESH : Staphylococcus aureus, Circular Dichroism, Peptide Conformation, Cell biology, medicine.anatomical_structure, Staphylococcus aureus, MESH: Membrane Proteins, Antitoxin, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MESH : Cell Membrane, MESH : Nuclear Magnetic Resonance, Biomolecular, Biology, complex mixtures, Microbiology, 03 medical and health sciences, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Nuclear Magnetic Resonance, Biomolecular, 030304 developmental biology, MESH: Humans, 030306 microbiology, MESH : Humans, Cell Membrane, RNA, Membrane Proteins, MESH : Circular Dichroism, Cell Biology, Oxidative Stress, chemistry, Membrane protein, MESH : Membrane Proteins, Cytoplasm, MESH : Protein Structure, Secondary, Peptides, DNA, MESH: Cell Membrane
Abstract

International audience; We report a functional type I toxin-antitoxin (TA) module expressed by a human pathogen, Staphylococcus aureus. TA systems consist of stable toxins and labile antitoxins encoded within small genetic modules widespread in eubacteria and archaea. TA genes provide stress adaptation and protection against DNA loss or invasion. The genes encoding the SprA1 toxic peptide (PepA1) and the SprA1(AS) RNA antitoxin are within a pathogenicity island on opposite strands and possess a 3' overlap. To prevent peptide toxicity during S. aureus growth, PepA1 expression from stable (half-life > 3 h) SprA1 is repressed by elevated amounts of unstable (half-life = ∼10 mn) SprA1(AS). In vivo, PepA1 localizes at the bacterial membrane and triggers S. aureus death. Based on NMR and CD data, its solution structure was solved and is a long bent, interrupted helix. Molecular dynamics simulations indicate that PepA1 compaction and helical content fluctuate in accordance with its cytoplasm or membrane location. When inserted into the S. aureus membrane, the PepA1 conformation switches to a ∼7-nm-long continuous helix, presumably forming pores to alter membrane integrity. PepA1 expression is induced upon acidic and oxidative stresses by reducing SprA1(AS) levels. As an altruistic behavior during infection, some cells may induce the expression of that toxin that would facilitate departure from the host immune cells for spreading.

Published
2012
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5. Effect of oxidative stress on UDP-glucuronosyltransferases in rat astrocytes

Author

Alain Minn, Daniela Gradinaru, Jean-Marie Heydel, Anne-Laure Minn, Yves Artur, University of Medicine and Pharmacy, Carol Davila University, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Conseil Regional de Bourgogne, Dijon, France, EU-COST Action on Chemistry of Non-Enzymatic Protein Modifications [CM1001], Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Pharmacologie, and Université de Vandœuvre-lès-Nancy

Subjects
MESH : Oxidative Stress, MESH : RNA, Messenger, Antioxidant, Transcription, Genetic, medicine.medical_treatment, Toxicology, NAD(P)H:quinone oxidoreductase 1, MESH: Glucuronosyltransferase, Antioxidants, Substrate Specificity, Rats, Sprague-Dawley, 0302 clinical medicine, MESH: NADPH-Ferrihemoprotein Reductase, MESH: Glucuronides, NAD(P)H Dehydrogenase (Quinone), MESH : Catalysis, MESH: Animals, MESH : NAD(P)H Dehydrogenase (Quinone), Glucuronosyltransferase, Cells, Cultured, chemistry.chemical_classification, MESH : Cell Survival, 0303 health sciences, MESH : Substrate Specificity, MESH : Animals, Newborn, Cytochrome P450 reductase, General Medicine, MESH: Cell Survival, MESH: Pyridinium Compounds, MESH : Antioxidants, MESH: Cells, Cultured, Oxidative phosphorylation, Gene Expression Regulation, Enzymologic, MESH : Quinones, MESH : Glucuronides, 03 medical and health sciences, RNA, Messenger, Cell Shape, NADPH-Ferrihemoprotein Reductase, MESH : Oxidation-Reduction, MESH : Pyridinium Compounds, MESH: Naphthols, MESH : Glucuronosyltransferase, MESH: Antioxidants, MESH: Catalysis, chemistry, Oxidative stress, Astrocytes, Reactive Oxygen Species, 030217 neurology & neurosurgery, MESH: Oxidation-Reduction, Time Factors, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, MESH : Reactive Oxygen Species, NADPH:cytochrome P450 reductase, Pyridinium Compounds, Naphthols, MESH: Rats, Sprague-Dawley, Protein oxidation, medicine.disease_cause, MESH: Animals, Newborn, MESH: NAD(P)H Dehydrogenase (Quinone), Protein Carbonylation, MESH : Oxidants, MESH: Oxidants, Melatonin, MESH: Melatonin, MESH: Oxidative Stress, MESH : Melatonin, MESH : Rats, MESH: Gene Expression Regulation, Enzymologic, Quinones, MESH: Reactive Oxygen Species, Oxidants, Biochemistry, MESH : Protein Carbonylation, Oxidation-Reduction, UDP-glucuronosyltransferase, MESH : Time Factors, MESH: Protein Carbonylation, MESH: Rats, Cell Survival, MESH : Naphthols, Biology, Catalysis, MESH: Quinones, MESH : Gene Expression Regulation, Enzymologic, Glucuronides, MESH : Cells, Cultured, medicine, Animals, MESH: Cell Shape, 030304 developmental biology, MESH: RNA, Messenger, Reactive oxygen species, MESH: Transcription, Genetic, MESH: Time Factors, MESH : Astrocytes, MESH : Transcription, Genetic, MESH : Rats, Sprague-Dawley, Rats, MESH: Astrocytes, Animals, Newborn, MESH : NADPH-Ferrihemoprotein Reductase, MESH: Substrate Specificity, MESH : Animals, NAD+ kinase, MESH : Cell Shape, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

WOS:000309170300003; International audience; The present work reports data regarding effects of an induced oxidative stress on the mainly expressed isoforms of UDP-glucuronosyltransferases (UGTs) in the brain. UGT1A6 and UGT1A7 expression and enzymatic activities toward the 1-naphthol were analyzed in rat cultured astrocytes following the exposure for 48 h to redox-cycling xenobiotic compounds such as quinones and bipyridinium ions. The expression of NADPH:cytochrome P450 reductase and NAD(P)H:quinone oxidoreductase 1 (NQO1) was also investigated. Oxidative stress induced significant deleterious changes in astrocyte morphology, decreased cell viability and inhibited catalytic function of UGTs as a result of protein oxidation. Alternatively, in the surviving impaired astrocytes, oxidative conditions induced a significant overactivity and overexpression of xenobiotic detoxification enzymes, as adaptive response. These effects were significantly prevented by the presence of melatonin, suggesting its direct antioxidant action on reactive oxygen species, reflected further on the glucuronidation activity and transcriptional regulation of both UGT1A6 and UGT1A7. Results show that both catalytic properties of UGTs and the expression of UGT1A6, UGT1A7, NQO1 and NADPH:cytochrome P450 reductase in rat astrocytes are greatly influenced by the pro-oxidative environment. In conclusion, an experimental increase in oxidative cellular status could have both immediate and long term consequences on detoxification enzymatic system activity and expression.

Published
2012
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6. General oxidative stress during doxorubicin-induced cardiotoxicity in rats: Absence of cardioprotection and low antioxidant efficiency of alpha-lipoic acid

Author

Luc Rochette, Ségolène Gambert, Laurent Martin, Stéphanie Delemasure, Catherine Vergely, Carole Richard, Steliana Ghibu, Jean-Claude Guilland, Laboratoire de Physiopathologie et Pharmacologie Cardio-Métaboliques (U866, Lipides et nutrition, équipe 5) (LPPCM), Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Physiopathologie et épidémiologie cérébro-cardiovasculaire [Dijon] (PEC2), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Vergely, Catherine, and Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)

Subjects
Male, MESH : Oxidative Stress, Antioxidant, medicine.medical_treatment, MESH : Hematocrit, MESH : Thioctic Acid, Biochemistry, Antioxidants, 0302 clinical medicine, Superoxides, Ascitic Fluid, MESH: Animals, MESH : Body Weight, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Thioctic Acid, Cumulative dose, MESH: Heart Diseases, Heart, General Medicine, 3. Good health, MESH: Ascitic Fluid, [SDV] Life Sciences [q-bio], 030220 oncology & carcinogenesis, MESH : Ascitic Fluid, MESH: Hydrogen Peroxide, MESH : Antioxidants, MESH: Thioctic Acid, medicine.medical_specialty, Cardiotonic Agents, Cardiotoxins, MESH: Hematocrit, 03 medical and health sciences, MESH: Doxorubicin, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, In vivo, Rats, Wistar, [ SDV ] Life Sciences [q-bio], Myocardium, MESH: Antioxidants, Hydrogen Peroxide, MESH: Cardiotonic Agents, MESH : Organ Size, MESH: Body Weight, MESH: Heart, carbohydrates (lipids), Endocrinology, MESH: Liver, MESH : Superoxides, MESH: Organ Size, [SDV]Life Sciences [q-bio], MESH : Cardiotonic Agents, Ascorbic Acid, MESH: Superoxides, medicine.disease_cause, MESH: Eating, Eating, polycyclic compounds, MESH : Myocardium, MESH: Thiobarbituric Acid Reactive Substances, MESH: Ascorbic Acid, Antibiotics, Antineoplastic, MESH: Oxidative Stress, Chemistry, MESH : Rats, Organ Size, MESH : Antibiotics, Antineoplastic, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Hematocrit, Liver, MESH : Cardiotoxins, medicine.drug, MESH : Eating, MESH: Myocardium, Heart Diseases, MESH: Rats, MESH : Male, MESH : Thiobarbituric Acid Reactive Substances, MESH : Rats, Wistar, Thiobarbituric Acid Reactive Substances, Contractility, MESH : Heart, Internal medicine, medicine, TBARS, Animals, MESH : Doxorubicin, Doxorubicin, MESH: Antibiotics, Antineoplastic, MESH : Ascorbic Acid, 030304 developmental biology, Cardiotoxicity, Body Weight, MESH : Liver, MESH : Heart Diseases, MESH: Rats, Wistar, MESH: Male, Rats, Oxidative Stress, MESH: Cardiotoxins, MESH : Animals, MESH : Hydrogen Peroxide, Oxidative stress
Abstract

International audience; To evaluate the effects of alpha-lipoic acid (AL) in a model of doxorubicin (DOX)-induced cardiotoxicity, male Wistar rats were treated with DOX (1 mg/kg/d; 10 d) in combination or not with AL (50 mg/kg/d; 15 d). Plasma oxidative stress was determined by hydroperoxides (ROOH) and the ascorbyl radical/ascorbate ratio. One and two months later, the functional parameters of the hearts were determined in vivo by catheterization and cardiac oxidative stress was assessed by malonedialdehyde (MDA) and O₂*⁻ (dihydroethidium fluorescence) content in tissue. After two months, body weight was higher in the DOX-AL group than in DOX (+16%), but this was due to ascites. Histological liver alterations were observed in both the DOX and DOX-AL groups. Plasma ROOH concentrations decreased after 10 days of AL treatment, but were greater in both the DOX and DOX-AL groups. After two months, a decrease in the cardiac contractility index (-27% and -29%, respectively) and cardiac hypertrophy were observed in DOX and DOX-AL. These dysfunctions were associated with 1) a reduction in plasma ascorbate levels and an increase in the ascorbyl/ascorbate ratio and 2) an increase MDA and O₂*⁻ content in cardiac tissue. In conclusion, a cumulative dose of 10 mg/kg doxorubicin induced functional alterations in the heart associated with plasma and cardiac oxidative stress. The co-administration of the antioxidant compound AL had no beneficial effects in this situation.

Published
2012
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7. Reversal of age-related oxidative stress prevents hippocampal synaptic plasticity deficits by protecting D-serine-dependent NMDA receptor activation

Author

Haxaire, Coline, Turpin, Fabrice R, Potier, Brigitte, Kervern, Myriam, Sinet, Pierre-Marie, Barbanel, Gérard, Mothet, Jean-Pierre, Dutar, Patrick, Billard, Jean-Marie, Institut de psychiatrie et neurosciences (U894 / UMS 1266), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Psychiatrie et Neurosciences (U894), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Mobilités : Vieillissement, Pathologie, Santé (COMETE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Psychiatrie et Neurosciences (CPN - U894), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM) - Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS), and Aix Marseille Université (AMU) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH : Oxidative Stress, Male, Aging, MESH: Hippocampus, MESH: Rats, MESH : Male, MESH : Hippocampus, MESH : Rats, Wistar, Hippocampus, Receptors, N-Methyl-D-Aspartate, MESH: Acetylcysteine, Serine, Animals, MESH : Neuronal Plasticity, MESH: Aging, MESH: Animals, MESH: Lysine, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Neuronal Plasticity, MESH: Serine, Rats, Wistar, MESH: Receptors, N-Methyl-D-Aspartate, Neuronal Plasticity, MESH: Oxidative Stress, MESH : Rats, Lysine, MESH : Serine, MESH: Rats, Wistar, MESH : Aging, MESH: Male, Acetylcysteine, Rats, Oxidative Stress, MESH : Lysine, MESH : Acetylcysteine, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Animals, MESH : Receptors, N-Methyl-D-Aspartate
Abstract

International audience; Oxidative stress (OS) resulting from an imbalance between antioxidant defenses and the intracellular accumulation of reactive oxygen species (ROS) contributes to age-related memory deficits. While impaired synaptic plasticity in neuronal networks is thought to underlie cognitive deficits during aging, whether this process is targeted by OS and what the mechanisms involved are still remain open questions. In this study, we investigated the age-related effects of the reducing agent N-acetyl-L-cysteine (L-NAC) on the activation of the N-methyl-D-aspartate receptor (NMDA-R) by its co-agonist D-serine, because alterations in this mechanism contribute greatly to synaptic plasticity deficits in aged animals. Long-term dietary supplementation with L-NAC prevented oxidative damage in the hippocampus of aged rats. Electrophysiological recordings in the CA1 of hippocampal slices indicated that NMDA-R-mediated synaptic potentials and theta-burst-induced long-term potentiation (LTP) were depressed in aged animals, deficits that could be reversed by exogenous D-serine. Chronic treatment with L-NAC, but not acute application of the reducing agent, restored potent D-serine-dependent NMDA-R activation and LTP induction in aged rats. In addition, it is also revealed that the age-related decrease in D-serine levels and in the expression of the synthesizing enzyme serine racemase, which underlies the decrease in NMDA-R activation by the amino acid, was rescued by long-term dietary treatment with L-NAC. Our results indicate that protecting redox status in aged animals could prevent injury to the cellular mechanisms underlying cognitive aging, in part by maintaining potent NMDA-R activation through the D-serine-dependent pathway.

Published
2012
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8. Increased susceptibility to liver fibrosis with age is correlated with an altered inflammatory response

Author

Meriem Mahrouf-Yorgov, Claudine Cosson, Elsa Abdoun, Bernard Fromenty, Claudia Mitchell, Alexandra Collin de l'Hortet, Hélène Gilgenkrantz, Jacques-Emmanuel Guidotti, Abdelhamid Slama, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Biochimie [Bicêtre], Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre, Foie, métabolismes et cancer, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris-Sud - Paris 11 ( UP11 ) -Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Bicêtre, Hépatotoxicité et xénobiotiques, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) -Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Bicêtre

Subjects
MESH : Oxidative Stress, MESH: Inflammation, Liver Cirrhosis, Male, Aging, Necrosis, MESH : Carbon Tetrachloride, MESH : Hepatocytes, medicine.disease_cause, Liver disorder, MESH: Hepatocytes, Mice, 0302 clinical medicine, MESH : Chronic Disease, Fibrosis, MESH : Cell Proliferation, MESH: Aging, MESH: Animals, Carbon Tetrachloride, 0303 health sciences, MESH: Oxidative Stress, 3. Good health, medicine.anatomical_structure, Hepatocyte, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 030211 gastroenterology & hepatology, Disease Susceptibility, medicine.symptom, MESH: Liver Cirrhosis, MESH : Male, Hepatitis C virus, MESH: Disease Susceptibility, Context (language use), Inflammation, MESH : Mice, Inbred C57BL, Biology, MESH : Disease Susceptibility, 03 medical and health sciences, MESH: Mice, Inbred C57BL, MESH: Cell Proliferation, MESH : Mice, medicine, MESH : Liver Cirrhosis, Animals, Humans, MESH: Mice, 030304 developmental biology, Cell Proliferation, MESH: Necrosis, MESH : Inflammation, MESH: Humans, MESH: Carbon Tetrachloride, MESH: Chronic Disease, MESH : Humans, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, medicine.disease, MESH : Aging, MESH: Male, MESH : Necrosis, Mice, Inbred C57BL, Oxidative Stress, Immunology, Chronic Disease, Hepatocytes, MESH : Animals, Geriatrics and Gerontology, Oxidative stress
Abstract

International audience; It has been suggested that increasing age is correlated with an acceleration of the progression of liver fibrosis induced by various agents, such as hepatitis C virus or chronic alcohol consumption. However, the cellular and molecular changes underlying this predisposition are not entirely understood. In the context of an aging population, it becomes challenging to decipher the mechanisms responsible for this higher susceptibility of older individuals to this acquired liver disorder. To address this issue, we induced liver fibrosis by carbon tetrachloride (CCl(4)) chronic administration to 8-week- and 15-month-old mice. We confirmed that susceptibility to fibrosis development increased with age and showed that aging did not affect fibrosis resolution capacity. We then focused on the impairment of hepatocyte proliferation, oxidative stress, and inflammation as potential mechanisms accelerating the development of fibrosis in the elderly. We detected no inhibition of hepatocyte proliferation after CCl(4) injury in 15-month-old mice, whereas it was inhibited after a partial hepatectomy. Finally, we observed that, in a context in which liver oxidative stress was not differentially increased in both experimental groups, there was a higher recruitment of inflammatory cells, including mostly macrophages and lymphocytes, oriented toward a T helper 2 (T(H)2) response in older mice. Our data show that in conditions of equivalent levels of oxidative stress and maintained hepatocyte proliferative capacity, an increased inflammatory reaction mainly composed of CD4(+) lymphocytes and macrophages expressing T(H)2 cytokines is the main factor involved in the higher susceptibility to fibrosis with increasing age.

Published
2011
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9. Inflammation and oxidative stress in vertebrate host-parasite systems

Author

Sorci , Gabriele, Faivre , Bruno, Biogéosciences [Dijon] ( BGS ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )

Subjects
MESH : Oxidative Stress, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, MESH : Inflammation, MESH : Host-Parasite Interactions, MESH : Humans, MESH : Reactive Oxygen Species, MESH : Immunity, Innate, delayed costs, [ SDV.IMM.IA ] Life Sciences [q-bio]/Immunology/Adaptive immunology, MESH : Aging, ageing, nitric oxide, MESH : Homeostasis, MESH : Selection (Genetics), MESH : Animals, innate immunity, MESH : Reactive Nitrogen Species, reactive oxygen and nitrogen species, immune evasion
Abstract

13 pages; International audience; Innate, inflammation-based immunity is the first line of vertebrate defence against micro-organisms. Inflammation relies on a number of cellular and molecular effectors that can strike invading pathogens very shortly after the encounter between inflammatory cells and the intruder, but in a non-specific way. Owing to this non-specific response, inflammation can generate substantial costs for the host if the inflammatory response, and the associated oxygen-based damage, get out of control. This imposes strong selection pressure that acts to optimize two key features of the inflammatory response: the timing of activation and resolution (the process of downregulation of the response). In this paper, we review the benefits and costs of inflammation-driven immunity. Our aim is to emphasize the importance of resolution of inflammation as a way of maintaining homeostasis against oxidative stress and to prevent the 'horror autotoxicus' of chronic inflammation. Nevertheless, host immune regulation also opens the way to pathogens to subvert host defences. Therefore, quantifying inflammatory costs requires assessing (i) short-term negative effects, (ii) delayed inflammation-driven diseases, and (iii) parasitic strategies to subvert inflammation.

Published
2009
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10. Preferential sphingosine-1-phosphate enrichment and sphingomyelin depletion are key features of small dense HDL3 particles: relevance to antiapoptotic and antioxidative activities

Author

Martine Couturier, Amal Zerrad, Patrice Thérond, Anatol Kontush, M. John Chapman, Juliana A. de Souza, S. Chantepie, Anne Nègre-Salvayre, Dyslipoproteinémies et athérosclerose : Génétique, métabolisme et thérapeutique, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire de biochimie, Université Paris Descartes - Paris 5 (UPD5), Régulations cellulaires: lipidoses et atherosclerose, IFR 31 Louis Bugnard (IFR 31), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Simon, Marie Francoise, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR14-Institut National de la Santé et de la Recherche Médicale ( INSERM ), CHU Pitié-Salpêtrière [APHP], Université Paris Descartes - Paris 5 ( UPD5 ), Régulations cellulaires: lipidoses et atherosclerose., IFR 31 Louis Bugnard ( IFR 31 ), Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Toulouse [Toulouse]-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), and Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
MESH : Oxidative Stress, MESH: Oxidation-Reduction, MESH: Lipoproteins, HDL3, MESH : Dyslipidemias, Apoptosis, MESH : Cholesterol, LDL, 030204 cardiovascular system & hematology, MESH: Sphingomyelins, Antioxidants, chemistry.chemical_compound, 0302 clinical medicine, Reference Values, Sphingosine, MESH : Particle Size, MESH : Sphingosine, 0303 health sciences, MESH: Oxidative Stress, MESH: Reference Values, Lipoproteins, HDL3, Sphingomyelins, Biochemistry, MESH : Cholesterol, HDL, MESH : Antioxidants, lipids (amino acids, peptides, and proteins), Cholesterol Esters, MESH : Sensitivity and Specificity, MESH: Sphingosine, MESH: Cholesterol, HDL, Cardiology and Cardiovascular Medicine, Sphingomyelin, Oxidation-Reduction, MESH: Cholesterol, LDL, MESH : Sphingomyelins, MESH: Enzyme Activation, MESH : Cholesterol Esters, MESH: Probability, MESH : Probability, Phospholipid, MESH : Reference Values, Sensitivity and Specificity, MESH: Lysophospholipids, 03 medical and health sciences, Enzyme activator, Humans, Sphingosine-1-phosphate, MESH: Particle Size, Particle Size, Dyslipidemias, Probability, 030304 developmental biology, MESH : Oxidation-Reduction, MESH: Humans, Cholesterol, MESH: Apoptosis, Cholesterol, HDL, MESH : Humans, MESH: Antioxidants, MESH: Dyslipidemias, Cholesterol, LDL, Key features, Sphingolipid, MESH: Sensitivity and Specificity, Enzyme Activation, Oxidative Stress, chemistry, MESH : Lipoproteins, HDL3, MESH : Lysophospholipids, MESH: Cholesterol Esters, Molecular Profile, Lysophospholipids, MESH : Enzyme Activation, MESH : Apoptosis
Abstract

Objective— The purpose of this study was to define heterogeneity in the molecular profile of lipids, including sphingomyelin and sphingosine-1-phosphate, among physicochemically-defined HDL subpopulations and potential relevance to antiatherogenic biological activities of dense HDL3. Methods and Results— The molecular profile of lipids (cholesteryl esters, phospholipids, sphingomyelin, and sphingosine-1-phosphate) in physicochemically-defined normolipidemic HDL subpopulations was determined by high-performance liquid chromatography and gas chromatography. As HDL particle size and molecular weight decreased with increment in density, molar lipid content diminished concomitantly. On a % basis, sphingomyelin abundance diminished in parallel with progressive increase in HDL density from HDL2b (12.8%) to HDL3c (6.2%; P P r =0.73, P r =0.58, P Conclusions— An elevated sphingosine-1-phosphate/sphingomyelin ratio is an integral feature of small dense HDL3, reflecting enrichment in sphingosine-1-phosphate, a key antiapoptotic molecule, and depletion of sphingomyelin, a structural lipid with negative impact on surface fluidity and LCAT activity. These findings further distinguish the structure and antiatherogenic activities of small, dense HDL.

Published
2007
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11. Allopurinol, oxidative stress and intestinal permeability in patients with cirrhosis: an open-label pilot study

Author

Jean-Louis Frossard, Solange Bresson-Hadni, Olivier Golaz, Laurent Spahr, Pauline Amann, Ilse Kern, Antoine Hadengue, WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, Université de Franche-Comté ( UFC ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), and Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)

Subjects
Liver Cirrhosis, Male, MESH : Oxidative Stress, Alcoholic liver disease, Cirrhosis, MESH : Aged, Pilot Projects, medicine.disease_cause, Gastroenterology, chemistry.chemical_compound, 0302 clinical medicine, Malondialdehyde, Medicine, MESH : Female, Enzyme Inhibitors, Xanthine oxidase inhibitor, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, MESH: Aged, MESH : Intestines, 0303 health sciences, MESH: Middle Aged, MESH: Oxidative Stress, MESH : Pilot Projects, Middle Aged, MESH : Adult, 3. Good health, Intestines, MESH: Enzyme Inhibitors, MESH: Permeability, Female, 030211 gastroenterology & hepatology, MESH: Liver Cirrhosis, MESH: Intestines, medicine.drug, Adult, medicine.medical_specialty, MESH: Allopurinol, medicine.drug_class, Allopurinol, MESH : Male, MESH: Malondialdehyde, Permeability, 03 medical and health sciences, Internal medicine, Hypertension, Portal, Humans, MESH : Liver Cirrhosis, MESH : Middle Aged, MESH : Allopurinol, Xanthine oxidase, MESH : Hypertension, Portal, Aged, 030304 developmental biology, MESH : Malondialdehyde, MESH: Hypertension, Portal, MESH : Enzyme Inhibitors, MESH: Humans, Intestinal permeability, Hepatology, business.industry, MESH : Humans, MESH: Adult, MESH: Pilot Projects, medicine.disease, MESH: Male, Surgery, Oxidative Stress, chemistry, MESH : Permeability, business, MESH: Female, Oxidative stress
Abstract

International audience; BACKGROUND: Cirrhosis is associated with intestinal barrier failure, related in part to enterocytes oxidative damage via xanthine oxidase overactivity. Experimentally, allopurinol, a xanthine oxidase inhibitor, reduces enterocytes' damage and bacterial translocation. AIM: To assess the short-term effects of allopurinol on intestinal permeability, oxidative stress and endotoxin-dependent cytokines in patients with cirrhosis. METHODS: Nineteen patients with cirrhosis, in a stable condition (age: 56 years; Child A/B/C: 6/7/6; ascites: 12; alcoholic cirrhosis: 16/19; abstinence >2 weeks), were included. At baseline and day 10 of allopurinol 400 mg/day, intestinal permeability [lactulose/mannitol (Lac/Man) ratio test], oxidative stress (serum malondialdehyde), as well as TNF-soluble receptor-1, IL-6 and lipopolysaccharide-binding protein (which reflects exposition to endotoxin) were measured. RESULTS: Malondialdehyde decreased significantly (-23%, P

Published
2007
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12. Carotenoids modulate the trade-off between egg production and resistance to oxidative stress in zebra finches

Author

Carlos Alonso-Alvarez, Josiane Prost, Godefroy Devevey, Sophie Bertrand, Gabriele Sorci, Bruno Faivre, Parasitologie évolutive ( PE ), École normale supérieure - Paris ( ENS Paris ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Parasitologie évolutive (PE), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Lipides - Nutrition - Cancer (U866) (LNC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, École normale supérieure - Paris (ENS-PSL), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Ministère de la Recherche et des Technologies (France), Université de Bourgogne, and Ministerio de Educación, Cultura y Deporte (España)

Subjects
Male, 0106 biological sciences, Avian clutch size, MESH : Oxidative Stress, Antioxidant, Oviposition, medicine.medical_treatment, MESH: Dietary Supplements, Free radicals, MESH: Beak, Trade-off, medicine.disease_cause, MESH: Reproduction, 01 natural sciences, MESH : Oviposition, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, MESH: Animals, MESH : Female, MESH : Finches, Carotenoid, MESH: Oviposition, MESH : Body Weight, media_common, chemistry.chemical_classification, Sex Characteristics, 0303 health sciences, MESH: Oxidative Stress, Ecology, Reproduction, Beak, MESH : Reproduction, Breed, Zebra finch, Female, MESH: Finches, MESH: Sex Characteristics, media_common.quotation_subject, MESH : Male, Color, Zoology, [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Biology, 010603 evolutionary biology, 03 medical and health sciences, medicine, Animals, MESH : Dietary Supplements, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, MESH: Color, MESH : Carotenoids, Body Weight, MESH : Sex Characteristics, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Carotenoids, MESH: Male, MESH : Beak, MESH: Body Weight, MESH : Color, chemistry, Oxidative stress, Dietary Supplements, MESH: Carotenoids, Carotenoïds, Finches, MESH : Animals, Carotenoids/blood, Carotenoids/metabolism, Finches/physiology, Oviposition/physiology, Oxidative Stress/physiology, Reproduction/physiology, MESH: Female, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

The allocation of resources to reproduction and survival is a central question of studies of life history evolution. Usually, increased allocation to current reproduction is paid in terms of reduced future reproduction and/or decreased survival. However, the proximal mechanisms underlying the cost of reproduction are poorly understood. Recently, it has been shown that increased susceptibility to oxidative stress might be one of such proximate links between reproduction and self-maintenance. Organisms possess a range of antioxidant defenses, including endogenously produced molecules (e.g., enzymes) and compounds ingested with food (e.g., carotenoids). If reproductive effort increases the production of reactive oxygen species, the availability of antioxidant defenses may partly or fully counteract the free-radical damages. One could, therefore, expect that the trade-off between reproduction and oxidative stress is modulated by the availability of antioxidant defenses. We tested this hypothesis in zebra finches. We manipulated reproductive effort by either allowing or preventing pairs to breed. Within each breeding or non-breeding group, the availability of antioxidant compounds was manipulated by supplementing or not supplementing the drinking water with carotenoids. We found that although birds in the breeding and non-breeding groups did not differ in their resistance to oxidative stress (the breakdown of red blood cells submitted to a controlled free-radical attack), one aspect of breeding effort (i.e., the number of eggs laid by birds in both breeding and non-breeding groups) was negatively correlated with resistance to oxidative stress only in birds that did not benefit from a carotenoid-supplemented diet. This result therefore suggests that carotenoid availability can modulate the trade-off between reproduction and resistance to oxidative stress., Financial support was provided by the Ministère de la Recherche (ACI Jeunes Chercheurs to GS) and the Université de Bourgogne (BQR to GD, MG, JP and BF). CA-A was funded by Ministerio de Educación, Cultura y Deporte (Spain).

Published
2006
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13. Evolution of liver antioxidant status and iron implication during the development of deoxycorticosterone-saline hypertension in rats

Author

Lysiane Richert, Carol Courderot-Masuyer, Alain Berthelot, Malika Bouhaddi, Jacques Regnard, Fatiha Elhaïmeur, Laurence Nicod, Sophie Robin, Catherine Guyon, Fonctions et dysfonctions épithéliales - UFC (EA 4267) ( FDE ), Université de Franche-Comté ( UFC ), Ingénierie et biologie cellulaire et tissulaire ( IBCT (ex IFR133) ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Marqueurs pronostiques et facteurs de régulations des pathologies cardiaques et vasculaires - UFC ( PCVP / CARDIO ), Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ) -Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Fonctions et dysfonctions épithéliales - UFC (EA 4267) (FDE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Marqueurs pronostiques et facteurs de régulations des pathologies cardiaques et vasculaires - UFC ( EA 3920) (PCVP / CARDIO), and Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC)

Subjects
MESH : Oxidative Stress, MESH : Trace Elements, Antioxidant, Time Factors, MESH : Oxygen, Thiobarbituric acid, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, MESH: Desoxycorticosterone, Blood Pressure, MESH: Rats, Sprague-Dawley, 030204 cardiovascular system & hematology, Sodium Chloride, medicine.disease_cause, Biochemistry, Antioxidants, MESH: Hypertension, MESH : Iron, Lipid peroxidation, Rats, Sprague-Dawley, chemistry.chemical_compound, 0302 clinical medicine, Cytosol, MESH: Cytosol, MESH : Oxidants, MESH: Oxidants, MESH: Thiobarbituric Acid Reactive Substances, MESH: Animals, Desoxycorticosterone, MESH: Glutathione, 0303 health sciences, MESH: Iron, MESH: Oxidative Stress, biology, Chemistry, MESH : Rats, MESH : Cytosol, MESH : Hypertension, General Medicine, MESH: Blood Pressure, MESH : Desoxycorticosterone, MESH : Glutathione, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Oxidants, Glutathione, MESH : Sodium Chloride, Liver, MESH : Nitric Oxide, Catalase, Hypertension, MESH : Antioxidants, MESH: Oxygen, MESH : Time Factors, medicine.medical_specialty, MESH: Sodium Chloride, MESH: Rats, Iron, MESH: Trace Elements, MESH : Thiobarbituric Acid Reactive Substances, Nitric Oxide, Thiobarbituric Acid Reactive Substances, Nitric oxide, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, TBARS, medicine, MESH : Blood Pressure, Animals, 030304 developmental biology, Biochemistry (medical), MESH: Antioxidants, MESH: Time Factors, MESH : Liver, MESH : Rats, Sprague-Dawley, Rats, Trace Elements, Oxygen, Oxidative Stress, Blood pressure, Endocrinology, [ SDV.SP ] Life Sciences [q-bio]/Pharmaceutical sciences, MESH: Nitric Oxide, biology.protein, MESH : Animals, Oxidative stress, MESH: Liver
Abstract

International audience; Hypertension is known to be associated with an oxidative stress resulting from an imbalance of antioxidant defense mechanisms in various tissues. The purpose of this study was to investigate the relationship between the increase of arterial blood pressure, measured during the gradual development of experimental hypertension in deoxycorticosterone (DOCA)-salt-treated rats, and an early imbalance of liver antioxidant status. The levels of liver oxidant/antioxidant markers and iron were studied during the induction of hypertension in 3-, 6-, and 8-wk DOCA-salt-treated Sprague-Dawley rats. Hepatic antioxidant defenses were decreased as early as 3 wk of hypertensive treatment: the decrease of peroxidase-reductase-transferase and catalase activities was associated with a significant increase of thiobarbituric acid reactive substances (TBARS) levels. Liver oxidative stress increased until 6 wk and remained stable at 8 wk of DOCA-salt treatment. Concurrently, liver iron levels were increased at 6 wk and returned to normal values after 8 wk of hypertensive treatment. Iron seems to be an inductor of liver oxidative stress and responsible for the persistent oxidative stress, most likely through secondary free-radical release. Thus, our data (1) confirm that hypertension in DOCA-salt-treated rats might be a free-radical-dependent disease where hepatic oxidant/antioxidant imbalance is obviously involved from the beginning of blood pressure elevation and (2) suggest that the use of suitable iron chelators might reverse liver oxidative stress associated with the increase of blood pressure.

Published
2005
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14. Resveratrol: preventing properties against vascular alterations and ageing

Author

Dominique Delmas, Norbert Latruffe, Brigitte Jannin, Laboratoire de Biochimie Moléculaire et Cellulaire ( LBMC ), Université de Bourgogne ( UB ), Lipides - Nutrition - Cancer (U866) ( LNC ), and Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA )

Subjects
MESH : Oxidative Stress, Aging, Antioxidant, Platelet Aggregation, Arteriosclerosis, medicine.medical_treatment, Resveratrol, Pharmacology, medicine.disease_cause, Muscle, Smooth, Vascular, chemistry.chemical_compound, MESH : Vasodilation, MESH : Foam Cells, MESH : Platelet Aggregation, Stilbenes, MESH : Cardiovascular Diseases, MESH : Macrophages, chemistry.chemical_classification, Neovascularization, Pathologic, Phytoalexin, food and beverages, Vasodilation, Biochemistry, Cardiovascular Diseases, medicine.symptom, Biotechnology, Lipoproteins, Inflammation, Health Promotion, MESH : Lipoproteins, Biology, MESH : Stilbenes, MESH : Arteriosclerosis, medicine, Humans, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Reactive oxygen species, Mechanism (biology), Macrophages, MESH : Humans, MESH : Neovascularization, Pathologic, MESH : Muscle, Smooth, Vascular, MESH : Aging, Oxidative Stress, chemistry, Ageing, MESH : Health Promotion, Oxidative stress, Food Science, Foam Cells
Abstract

International audience; Cardiovascular diseases are the leading cause of death in developed countries where the common pathological substrate underlying this process is atherosclerosis. Several new concepts have emerged in relation to mechanisms that contribute to the regulation of the vascular diseases and associated inflammatory effects. Recently, potential antioxidants (vitamin E, polyphenols) have received much attention as potential anti-atherosclerotic agents. Among the polyphenols with health benefic properties, resveratrol, a phytoalexin of grape, seem to be a good candidate protecting the vascular walls from oxidation, inflammation, platelet aggregation, and thrombus formation. In this review, we focus on the mechanism of resveratrol cardiovascular benefic effects. We analyze, in relation with the different steps of atherosclerotic process, the resveratrol properties at multiple levels, such as cellular signaling, enzymatic pathways, apoptosis, and gene expression. We show and discuss the relationship with reactive oxygen species, regulation of pro-inflammatory genes including cycloxygenases and cytokines in molecular inflammatory and aging processes, and how the regulation of these activites by resveratrol can lead to a prevention of vascular diseases.

Published
2005
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15. Assessment of whole effluent toxicity on aquatic snails: Bioaccumulation of Cr, Zn, and Fe, and individual effects in bioassays

Author

Michael Coeurdassier, Pierre-Marie Badot, Annette de Vaufleury, Renaud Scheifler, Nadia Crini, Unité sous contrat biologie environnementale, Institut National de la Recherche Agronomique (INRA)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Institut National de la Recherche Agronomique ( INRA ) -Université de Franche-Comté ( UFC ), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)

Subjects
MESH : Lymnaea, MESH : Oxidative Stress, Chromium, MESH: Hydrogen-Ion Concentration, Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], 0211 other engineering and technologies, [ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/Ecotoxicology, MESH : Dose-Response Relationship, Drug, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Waste Disposal, Fluid, MESH: Zinc, MESH : Iron, Lymnaeidae, MESH: Dose-Response Relationship, Drug, Toxicology, MESH : Fertility, Malondialdehyde, Gastropoda, Bioassay, MESH: Animals, LYMNAEA PALUSTRIS, Lymnaea, MESH : Waste Disposal, Fluid, MESH: Iron, MESH: Oxidative Stress, biology, Hydrogen-Ion Concentration, 6. Clean water, Zinc, MESH: Water Pollutants, Chemical, Environmental chemistry, Bioaccumulation, Toxicity, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, MESH : Water Pollutants, Chemical, Iron, MESH: Malondialdehyde, GASTROPODA, MESH: Chromium, MESH : Hydrogen-Ion Concentration, MESH: Lymnaea, Environmental Chemistry, Ecotoxicology, Animals, Effluent, 0105 earth and related environmental sciences, MESH : Malondialdehyde, 021110 strategic, defence & security studies, Dose-Response Relationship, Drug, MESH: Fertility, MESH : Zinc, biology.organism_classification, Oxidative Stress, Fertility, MESH : Chromium, MESH : Animals, MESH: Waste Disposal, Fluid, Water Pollutants, Chemical
Abstract

International audience; We used a freshwater gastropod, Lymnaea palustris, in chronic bioassays to assess the toxicity of an industrial effluent containing high levels of metals, particularly Cr, Zn, and Fe. Adult snails were exposed for four weeks to different concentrations of effluent sampled at three successive treatment steps (crude effluent, effluent after physicochemical treatment, and after biological treatment). Dose-dependent responses reflecting exposure (metal bioaccumulation) and effects on survival, fecundity, and malondialdehyde production (a proxy for oxidative stress) were investigated. We found that Cr and Zn were accumulated in snail tissues, whereas Fe was regulated. Body concentrations of Cr and Zn decreased along the effluent-treatment gradient, particularly after the physicochemical treatment. For controls versus treatments, no effect on malondialdehyde production was detected. Significant effects were noted for fecundity. The number of eggs per individual decreased for snails exposed to 20, 30, and 40% concentrations of physicochemically treated effluent and for snails exposed to an 80% concentration of the biologically treated effluent. A hormetic effect on the number of eggs per individual was observed for snails exposed to 10 and 20% concentrations of the effluent that had been biologically treated. Deleterious effects of the effluent on L. palustris fecundity were not correlated with high internal concentrations of metals in the snails, suggesting that toxicity resulted from other factors.

Published
2005
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16. The stress proteome of Enterococcus faecalis

Author

J C, Giard, J M, Laplace, A, Rincé, V, Pichereau, A, Benachour, C, Leboeuf, S, Flahaut, Y, Auffray, A, Hartke, Laboratoire de Microbiologie de l’Environnement (LME), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Microbiologie de l’Environnement ( LME ), Institut National de la Recherche Agronomique ( INRA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Laboratoire des Sciences de l'Environnement Marin (LEMAR) ( LEMAR ), Institut de Recherche pour le Développement ( IRD ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Université de Brest ( UBO ) -Institut Universitaire Européen de la Mer ( IUEM ), Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université de Brest ( UBO ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Université de Brest ( UBO ) -Centre National de la Recherche Scientifique ( CNRS ), and Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH : Oxidative Stress, MESH : Molecular Sequence Data, Proteome, MESH: Sequence Homology, Amino Acid, MESH : Enzymes, Sequence Homology, MESH : Blotting, Western, MESH: Amino Acid Sequence, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH : Mercuric Chloride, MESH: Silver Staining, Cadmium Chloride, Enterococcus faecalis, MESH : Bacterial Proteins, MESH : Silver Staining, Electrophoresis, Gel, Two-Dimensional, MESH : Enterococcus faecalis, Databases, Protein, MESH: Bacterial Proteins, MESH: Cadmium Chloride, Gel, MESH: Gene Expression Regulation, Bacterial, MESH: Oxidative Stress, Blotting, MESH : Amino Acid Sequence, Bacterial, Sodium Dodecyl Sulfate, MESH : Sequence Homology, Amino Acid, Enzymes, DNA-Binding Proteins, MESH: Glucose, MESH: Proteome, Amino Acid, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MESH: Repressor Proteins, Two-Dimensional, Mercuric Chloride, MESH: Hydrogen Peroxide, MESH : DNA-Binding Proteins, MESH : Repressor Proteins, Western, MESH : Gene Expression Regulation, Bacterial, Electrophoresis, MESH: Sodium Dodecyl Sulfate, MESH: Databases, Protein, Silver Staining, MESH: Enterococcus faecalis, MESH: Terminology as Topic, MESH : Cadmium Chloride, Blotting, Western, Detergents, Molecular Sequence Data, MESH : Acids, MESH: Enzymes, MESH : Proteome, MESH : Open Reading Frames, MESH: Bile Acids and Salts, MESH: Acids, Bile Acids and Salts, Databases, Open Reading Frames, Bacterial Proteins, Terminology as Topic, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MESH: Blotting, Western, Amino Acid Sequence, MESH : Databases, Protein, MESH: Mercuric Chloride, MESH : Sodium Dodecyl Sulfate, MESH: Molecular Sequence Data, Sequence Homology, Amino Acid, MESH : Glucose, Protein, Gene Expression Regulation, Bacterial, MESH : Detergents, MESH : Electrophoresis, Gel, Two-Dimensional, Hydrogen Peroxide, MESH: Open Reading Frames, MESH: Electrophoresis, Gel, Two-Dimensional, MESH : Terminology as Topic, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH : Bile Acids and Salts, Repressor Proteins, Oxidative Stress, Glucose, Gene Expression Regulation, MESH : Hydrogen Peroxide, Acids, MESH: DNA-Binding Proteins, MESH: Detergents
Abstract

International audience; Enterococcus faecalis is a resident bacterium of the intestinal tract of humans and animals. This bacterium can be responsible for serious diseases and is one of the largest causes of hospital-based infections. This hardy organism resists many kinds of stresses and is used as a major indicator of the hygienic quality of food, milk, and drinking water. On the other side, enterococci seem to have beneficial role in the development of cheese aroma and are added in certain starter cultures. Since ten years, our laboratory has used the two-dimensional electrophoresis (2-DE) technique to study the response of E. faecalis to physical or chemical stresses as well as to glucose and total starvation. Twenty-seven protein spots on 2-D gels have been identified by N-terminal sequencing or Western blotting which make up the first proteome database of this species. The proteins were classified in four different groups according to their function and their regulation. The first group comprises well-characterized proteins with known protective functions towards stresses. The second group contains enzymes of catabolic pathways. Their implication in stress resistance seems not obvious. A third group are proteins induced in glucose-starved cells belonging to the CcpA regulon. Induction of these enzymes under starvation may serve to increase the scavenging capacity of the cells for nutrients or may be important to mobilize endogenous energetic reserves. Lastly, nine N-terminal amino acid sequences or open reading frames (ORF) showed no homologies with sequences in databases. A comprehensive description of stress proteins of E. faecalis and analysis of their patterns of expression under different environmental conditions would greatly increase our understanding of the molecular mechanisms underlying the extraordinary capacity of this bacterium to survive under hostile conditions.

Published
2001
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17. JunD Reduces Tumor Angiogenesis by Protecting Cells from Oxidative Stress

Author

Jacques Pouysségur, Damien Gerald, Denise A. Chan, Fatima Mechta-Grigoriou, Amato J. Giaccia, Moshe Yaniv, Edurne Berra, Daniel Mansuy, Yves Frapart, Expression Génétique et Maladies, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques ( LCBPT - UMR 8601 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Centre National de la Recherche Scientifique ( CNRS ), Dept of Radiation Oncology, Stanford University [Stanford], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Radiation Oncology [Stanford], Stanford Medicine, Stanford University-Stanford University, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), and Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Vascular Endothelial Growth Factor A, MESH : Oxidative Stress, MESH : Transcription Factors, Proto-Oncogene Proteins c-jun, Angiogenesis, Cell, MESH : Reactive Oxygen Species, medicine.disease_cause, Antioxidants, MESH : Iron, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, Neovascularization, 0302 clinical medicine, Neoplasms, MESH: Up-Regulation, MESH: Animals, MESH: Neoplasms, MESH : Up-Regulation, G alpha subunit, chemistry.chemical_classification, Regulation of gene expression, MESH: Iron, 0303 health sciences, MESH: Oxidative Stress, MESH : Procollagen-Proline Dioxygenase, Neovascularization, Pathologic, integumentary system, MESH: Reactive Oxygen Species, MESH: Transcription Factors, MESH: Gene Expression Regulation, Neoplastic, Up-Regulation, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, 030220 oncology & carcinogenesis, MESH : Vascular Endothelial Growth Factor A, MESH: Hydrogen Peroxide, MESH : Antioxidants, medicine.symptom, MESH: ras Proteins, MESH : Proto-Oncogene Proteins c-jun, MESH: Procollagen-Proline Dioxygenase, MESH : Hypoxia-Inducible Factor 1, alpha Subunit, MESH : Gene Expression Regulation, Neoplastic, Iron, Procollagen-Proline Dioxygenase, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, MESH: Hypoxia-Inducible Factor 1, alpha Subunit, General Biochemistry, Genetics and Molecular Biology, Dioxygenases, Hypoxia-Inducible Factor-Proline Dioxygenases, 03 medical and health sciences, MESH : Dioxygenases, MESH: Dioxygenases, medicine, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Transcription factor, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030304 developmental biology, Reactive oxygen species, MESH: Humans, MESH: Proto-Oncogene Proteins c-jun, Biochemistry, Genetics and Molecular Biology(all), MESH: Vascular Endothelial Growth Factor A, MESH: Antioxidants, MESH : Humans, MESH : Neovascularization, Pathologic, Hydrogen Peroxide, Hypoxia-Inducible Factor 1, alpha Subunit, Molecular biology, MESH : Neoplasms, Oxidative Stress, chemistry, ras Proteins, MESH : Animals, MESH : Hydrogen Peroxide, MESH : ras Proteins, Reactive Oxygen Species, MESH: Neovascularization, Pathologic, Oxidative stress, Transcription Factors
Abstract

International audience; Reactive oxygen species (ROS) are implicated in the pathophysiology of various diseases, including cancer. In this study, we show that JunD, a member of the AP-1 family of transcription factors, reduces tumor angiogenesis by limiting Ras-mediated production of ROS. Using junD-deficient cells, we demonstrate that JunD regulates genes involved in antioxidant defense, H2O2 production, and angiogenesis. The accumulation of H2O2 in junD-/- cells decreases the availability of FeII and reduces the activity of HIF prolyl hydroxylases (PHDs) that target hypoxia-inducible factors-alpha (HIFalpha) for degradation. Subsequently, HIF-alpha proteins accumulate and enhance the transcription of VEGF-A, a potent proangiogenic factor. Our study uncovers the mechanism by which JunD protects cells from oxidative stress and exerts an antiangiogenic effect. Furthermore, we provide new insights into the regulation of PHD activity, allowing immediate reactive adaptation to changes in O2 or iron levels in the cell.

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