1. Infection-Mediated Priming of Phagocytes Protects against Lethal Secondary Aspergillus fumigatus Challenge
- Author
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Oumaïma Ibrahim-Granet, Amélie Savers, Bernhard Ryffel, Marianna Parlato, Gérard Eberl, Grégory Jouvion, Jean-Marc Cavaillon, Orhan Rasid, Matthias Brock, Cytokines et Inflammation, Institut Pasteur [Paris], School of Life Sciences, University of Nottingham, UK ( UON ), Imagine - Institut des maladies génétiques ( IMAGINE - U1163 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Histopathologie humaine et Modèles animaux, Immunologie et Neurogénétique Expérimentales et Moléculaires ( INEM ), Université d'Orléans ( UO ) -Centre National de la Recherche Scientifique ( CNRS ), Développement des Tissus Lymphoïdes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), This work was supported by funding from Institut Pasteur PTR 468 to OIG and AS, and the Transregio 124 'FungiNet' project A3 to AS and MB., University of Nottingham, UK (UON), Imagine - Institut des maladies génétiques (IMAGINE - U1163), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunologie et Neurogénétique Expérimentales et Moléculaires (INEM), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and BENEDIC, Bénédicte
- Subjects
MESH : Bone Marrow ,MESH : Spores, Fungal ,medicine.medical_treatment ,MESH : Cytokines ,MESH: Flow Cytometry ,Pathology and Laboratory Medicine ,Aspergillosis ,MESH: Phagocytes ,Aspergillus fumigatus ,Animal Cells ,MESH : Aspergillosis ,MESH: Animals ,lcsh:Science ,skin and connective tissue diseases ,Lung ,Disease Resistance ,Fungal Pathogens ,Phagocytes ,Innate Immune System ,MESH: Cytokines ,Flow Cytometry ,3. Good health ,[SDV] Life Sciences [q-bio] ,Aspergillus ,Blood ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Aspergillus Fumigatus ,Medical Microbiology ,Spectrophotometry ,Cytokines ,MESH: Bone Marrow ,Cytophotometry ,Cellular Types ,MESH : Receptors, Interleukin-8B ,Immune Cells ,Phagocytosis ,Immunology ,030106 microbiology ,Microbiology ,03 medical and health sciences ,Microbial Pathogens ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Blood Cells ,[ SDV ] Life Sciences [q-bio] ,Macrophages ,lcsh:R ,MESH: Host-Pathogen Interactions ,Organisms ,Biology and Life Sciences ,Molecular Development ,medicine.disease ,Molds (Fungi) ,Mice, Inbred C57BL ,030104 developmental biology ,lcsh:Q ,Developmental Biology ,0301 basic medicine ,Chemokine ,Neutrophils ,[SDV]Life Sciences [q-bio] ,lcsh:Medicine ,MESH: Mice, Knockout ,Receptors, Interleukin-8B ,White Blood Cells ,Spectrum Analysis Techniques ,Bone Marrow ,[ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology ,Immune Physiology ,Medicine and Health Sciences ,MESH : Lectins, C-Type ,CXC chemokine receptors ,MESH : Host-Pathogen Interactions ,Mice, Knockout ,Mice, Inbred BALB C ,Multidisciplinary ,Bone Marrow/immunology/metabolism ,biology ,MESH: Receptors, Interleukin-8B ,Hematology ,Spores, Fungal ,MESH: Chemokines ,Body Fluids ,Cytokine ,Cell Processes ,Host-Pathogen Interactions ,MESH : Phagocytes ,Aspergillus fumigatus/immunology ,Chemokines ,Pathogens ,Anatomy ,MESH: Aspergillus fumigatus ,Research Article ,MESH : Flow Cytometry ,MESH : Lung ,MESH: Mice, Inbred BALB C ,MESH: Disease Resistance ,Mycology ,MESH : Mice, Inbred C57BL ,Research and Analysis Methods ,Immune system ,MESH: Mice, Inbred C57BL ,MESH: Spores, Fungal ,medicine ,MESH : Disease Resistance ,Animals ,Lectins, C-Type ,MESH: Lung ,MESH : Chemokines ,MESH: Aspergillosis ,MESH : Mice, Inbred BALB C ,Innate immune system ,Fungi ,Cell Biology ,Aspergillosis/immunology/microbiology ,biology.organism_classification ,Immune System ,MESH : Aspergillus fumigatus ,physiology ,biology.protein ,MESH : Mice, Knockout ,MESH : Animals ,MESH: Lectins, C-Type - Abstract
International audience; Phagocytes restrict the germination of Aspergillus fumigatus conidia and prevent the establishment of invasive pulmonary aspergillosis in immunecompetent mice. Here we report that immunecompetent mice recovering from a primary A. fumigatus challenge are protected against a secondary lethal challenge. Using RAGγc knock-out mice we show that this protection is independent of T, B and NK cells. In protected mice, lung phagocytes are recruited more rapidly and are more efficient in conidial phagocytosis and killing. Protection was also associated with an enhanced expression of CXCR2 and Dectin-1 on bone marrow phagocytes. We also show that protective lung cytokine and chemokine responses are induced more rapidly and with enhanced dynamics in protected mice. Our findings support the hypothesis that following a first encounter with a non-lethal dose of A. fumigatus conidia, the innate immune system is primed and can mediate protection against a secondary lethal infection.
- Published
- 2016
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