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3 results on '"MESH : Rats, Long-Evans"'

1. Decreasing dietary linoleic acid promotes long chain omega-3 fatty acid incorporation into rat retina and modifies gene expression

Author

Stéphane Grégoire, Lionel Bretillon, Catherine Creuzot-Garcher, Niyazi Acar, Bertrand Bardet, Emilie Simon, Alain M. Bron, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), INRA (Human Nutrition Department, France), Laboratoires Horus Pharma (Saint Laurent du Var, France) 29000305, and Regional Council of Burgundy (France) 29000362

Subjects
CD36 Antigens, Male, MESH : RNA, Messenger, MESH: 5-Lipoxygenase-Activating Proteins, MESH : Receptors, LDL, MESH: Electroretinography, 0302 clinical medicine, MESH: Fatty Acids, Omega-3, MESH: Animals, MESH : Retinal Ganglion Cells, chemistry.chemical_classification, 0303 health sciences, MESH : Gene Expression Regulation, MESH : Electroretinography, MESH: Retina, MESH: Chromatography, Gas, MESH: Dietary Fats, Unsaturated, docosahexaenoic acid, polyunsaturated fatty acid, Sensory Systems, 3. Good health, nutrition, MESH: Photic Stimulation, Adipose Tissue, MESH: Adipose Tissue, medicine.medical_specialty, Chromatography, Gas, macular degeneration, Linoleic acid, MESH : Arachidonate 12-Lipoxygenase, Arachidonate 12-Lipoxygenase, MESH : Adipose Tissue, MESH: Arachidonate 12-Lipoxygenase, pufa, 03 medical and health sciences, MESH : Dietary Fats, Unsaturated, lipid, Electroretinography, Rats, Long-Evans, RNA, Messenger, MESH: Linoleic Acid, MESH: Antigens, CD36, MESH : Retina, Fatty acid, MESH: Retinal Ganglion Cells, eye diseases, Ophthalmology, Endocrinology, chemistry, MESH: Receptors, LDL, 030221 ophthalmology & optometry, ATP-Binding Cassette Transporters, n 3, MESH: Female, Photic Stimulation, MESH: Liver, Retinal Ganglion Cells, retina, MESH : 5-Lipoxygenase-Activating Proteins, genetic structures, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, retinal pigment epithelium, electroretinogram, MESH : Photic Stimulation, Adipose tissue, angiogenesis, chemistry.chemical_compound, MESH : Female, MESH : Rats, Long-Evans, 2. Zero hunger, medicine.diagnostic_test, MESH : Rats, MESH: Real-Time Polymerase Chain Reaction, MESH: Gene Expression Regulation, MESH : Antigens, CD36, medicine.anatomical_structure, Liver, ALOX12, Biochemistry, MESH: ATP-Binding Cassette Transporters, Female, ATP Binding Cassette Transporter 1, Polyunsaturated fatty acid, MESH : Fatty Acids, Omega-3, MESH: Rats, brain, MESH : Male, 5-Lipoxygenase-Activating Proteins, MESH : Real-Time Polymerase Chain Reaction, rhesus monkey, Biology, Real-Time Polymerase Chain Reaction, MESH : Chromatography, Gas, Linoleic Acid, Cellular and Molecular Neuroscience, Dietary Fats, Unsaturated, MESH : Linoleic Acid, MESH: Rats, Long-Evans, Internal medicine, Fatty Acids, Omega-3, medicine, Animals, MESH : ATP-Binding Cassette Transporters, Omega 3 fatty acid, MESH: RNA, Messenger, 030304 developmental biology, deficient diet, Retinal pigment epithelium, MESH : Liver, MESH: Male, Rats, Gene Expression Regulation, Receptors, LDL, gene expression, MESH : Animals, sense organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; Age-related macular degeneration (AMD) may be partially prevented by dietary habits privileging the consumption of ω3 long chain polyunsaturated fatty acids (ω3s) while lowering linoleic acid (LA) intake. The present study aimed to document whether following these epidemiological guidelines would enrich the neurosensory retina and RPE with ω3s and modulate gene expression in the neurosensory retina. Rat progenitors and pups were fed with diets containing low or high LA, and low or high ω3s. After scotopic single flash and 8-Hz-Flicker electroretinography, rat pups were euthanized at adulthood. The fatty acid profile of the neurosensory retina, RPE, liver, adipose tissue and plasma was analyzed using gas chromatography. Gene expression was analyzed with real-time PCR in the neurosensory retina. Diets rich in ω3s efficiently improved the incorporation of ω3s into the organs and tissues. This raising effect was magnified by lowering LA intake. Compared to a diet with high LA and low ω3s, low LA diets significantly upregulated LDL-receptor gene expression. Similar but not significant upregulation of CD36, ABCA1, ALOX5 and ALOX12 gene expression was observed in rats fed with low LA. No effect was observed on retinal function. Increasing the intake in ω3s and lowering LA improved the enrichment with ω3s of the tissues, including the neurosensory retina and RPE, and upregulated genes involved in lipid trafficking in the neurosensory retina. Those results consistently reinforced the beneficial role of ω3s in the prevention of AMD, especially when the diet contained low levels of LA, as suggested from epidemiological data.

Published
2011
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2. Production of neurospheres from mammalian Müller cells in culture

Author

Claudine Versaux-Botteri, David Hicks, Germaine Michel, Julie Monnin, Nadège Morand-Villeneuve, Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) (NEURO), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Franche-Comté (UFC), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Etablissement français du sang [Bourgogne-France-Comté] (EFS [Bourgogne-France-Comté])-Université de Franche-Comté (UFC), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481) (NEURO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS BFC)-Université de Franche-Comté (UFC), Laboratoire de Neurosciences Intégratives et Cliniques - UFC (EA 481) ( NEURO ), Université Bourgogne Franche-Comté [COMUE] ( UBFC ) -Université de Franche-Comté ( UFC ), Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Ingénierie et biologie cellulaire et tissulaire ( IBCT (ex IFR133) ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Laboratoire de Neurosciences Intégratives et Cliniques - UFC ( NEURO ), and Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC )

Subjects
MESH: Epidermal Growth Factor, MESH: Animals, Newborn, chemistry.chemical_compound, 0302 clinical medicine, MESH: Glial Fibrillary Acidic Protein, MESH: Animals, MESH : Rats, Long-Evans, Cells, Cultured, MESH : Epidermal Growth Factor, 0303 health sciences, Glial fibrillary acidic protein, biology, MESH : Rats, MESH : Animals, Newborn, MESH : Neuroglia, MESH: Retina, Stem Cells, General Neuroscience, MESH : Glial Fibrillary Acidic Protein, Cell biology, medicine.anatomical_structure, MESH : Stem Cells, Neuroglia, MESH: Neuroglia, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Carrier Proteins, MESH: Cells, Cultured, MESH: Rats, MESH: Carrier Proteins, MESH: Stem Cells, Retina, 03 medical and health sciences, MESH: Rats, Long-Evans, Neurosphere, MESH : Cells, Cultured, Glial Fibrillary Acidic Protein, medicine, Animals, Rats, Long-Evans, Progenitor cell, 030304 developmental biology, Epidermal Growth Factor, MESH : Retina, Retinal, Nestin, In vitro, Culture Media, Rats, Animals, Newborn, chemistry, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Culture Media, biology.protein, MESH : Culture Media, MESH : Animals, sense organs, Carrier Proteins, Neuroscience, 030217 neurology & neurosurgery
Abstract

International audience; In lower vertebrates, like fishes and amphibians, retina is able to self-regenerate whereas Mammalian retina has lost this property. Nevertheless, recently, it has turned out that retinal glial Müller cells were playing a role in neuronal regeneration of the adult rodent retina, in case of acute damages, by dedifferentiating and redifferentiating in glial and neural cells. The purpose of this study was to analyse the ability of mammalian Müller cells for forming neurospheres. First of all, rats Müller cells were isolated in a primary culture. Second, these cells were resuspended in two different culture media: the cells cultures in the Neurobasal-A medium kept a typical Müller cells morphology even after 15 days of EGF treatment, and the cells plated in the DMEM-F12 medium formed neurospheres from the third day in culture. The neurosphere cells expressed nestin, cellular retinaldehyde binding protein (CRALBP) and glial fibrillary acidic protein (GFAP). These results showed our capacity to isolate and propagate Müller cells-derived progenitor cells. Moreover, it allows us to control the number of progenitor cells and, in the future, to study their differentiation capacity.

Published
2007
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3. CXCR3 antagonism of SDF-1(5-67) restores trabecular function and prevents retinal neurodegeneration in a rat model of ocular hypertension

Author

William Rostène, Julie Frugier, Françoise Baleux, David Godefroy, Isabelle Célérier, Serge Picaud, Françoise Brignole-Baudouin, Julie Degardin, Jeffrey K. Harrison, Christophe Baudouin, José Sahel, Alexandre Denoyer, Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche des Cordeliers - Equipe 20 'ingénierie des connaissances en santé', Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Florida [Gainesville] (UF), Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Chimie des biomolécules, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Centre National de la Recherche Scientifique ( CNRS ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ), CIC Quinze-Vingts, Assistance publique - Hôpitaux de Paris (AP-HP)-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Department of Pharmacology & Therapeutics, College of Medicine, University of Florida, Laboratory of Toxicology, Faculty of Pharmaceutical and Biological Sciences, Université Paris Descartes - Paris 5 ( UPD5 ), Chimie des Biomolécules, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), This work was supported by INSERM grants for AD, DG, JD, WR and CB, by a UPMC grant for IC and JF, and by an NIH grant for JKH (AI058256)., Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP) ( APHP ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Faculté de Pharmacie de Paris - Université Paris Descartes (UPD5 Pharmacie), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Godefroy, David

Subjects
Retinal degeneration, Male, Visual System, Ocular hypertension, Apoptosis, MESH : Cytoprotection, MESH : Receptors, CXCR4, MESH : Receptors, CXCR3, 0302 clinical medicine, MESH: Animals, lcsh:Science, MESH: Stress, Physiological, MESH : Glaucoma, 0303 health sciences, Neurodegeneration, Anatomy, MESH : Chemokine CXCL12, Sensory Systems, 3. Good health, [SDV] Life Sciences [q-bio], MESH : Retinal Degeneration, Medicine, MESH: Chemokine CXCL12, MESH: Glaucoma, medicine.medical_specialty, MESH: Enzyme Activation, Receptors, CXCR4, Receptors, CXCR3, MESH: Retinal Degeneration, Ocular Anatomy, Caspase 3, MESH: Receptors, CXCR4, MESH: Receptors, CXCR3, 03 medical and health sciences, Humans, Rats, Long-Evans, MESH: Vision, Ocular, Biology, Intraocular Pressure, MESH: Humans, [ SDV ] Life Sciences [q-bio], lcsh:R, MESH : Humans, Glaucoma, medicine.disease, MESH : Disease Models, Animal, eye diseases, Chemokine CXCL12, MESH: Cell Line, MESH: Ocular Hypertension, Enzyme Activation, MESH: Cytoprotection, Ophthalmology, Endocrinology, chemistry, 030221 ophthalmology & optometry, Rat, lcsh:Q, Ocular Hypertension, Trabecular meshwork, MESH: Disease Models, Animal, MESH : Apoptosis, Neuroscience, MESH : Cell Line, Intraocular pressure, Anatomy and Physiology, genetic structures, [SDV]Life Sciences [q-bio], MESH : Vision, Ocular, lcsh:Medicine, chemistry.chemical_compound, MESH: Caspase 3, Molecular Cell Biology, MESH : Caspase 3, MESH : Rats, Long-Evans, MESH : Intraocular Pressure, Multidisciplinary, Cell Death, MESH : Rats, Retinal Degeneration, Animal Models, MESH : Trabecular Meshwork, medicine.anatomical_structure, Research Article, MESH: Rats, MESH : Male, Cell Line, Model Organisms, MESH: Intraocular Pressure, MESH: Rats, Long-Evans, Stress, Physiological, Trabecular Meshwork, Ocular System, Internal medicine, medicine, MESH : Ocular Hypertension, Animals, MESH : Stress, Physiological, Vision, Ocular, 030304 developmental biology, MESH: Apoptosis, [ SDV.BC.BC ] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Retinal, MESH: Male, Rats, MESH: Trabecular Meshwork, Disease Models, Animal, Cytoprotection, MESH : Animals, sense organs, MESH : Enzyme Activation
Abstract

International audience; Glaucoma, the most common cause of irreversible blindness, is a neuropathy commonly initiated by pathological ocular hypertension due to unknown mechanisms of trabecular meshwork degeneration. Current antiglaucoma therapy does not target the causal trabecular pathology, which may explain why treatment failure is often observed. Here we show that the chemokine CXCL12, its truncated form SDF-1(5-67), and the receptors CXCR4 and CXCR3 are expressed in human glaucomatous trabecular tissue and a human trabecular cell line. SDF-1(5-67) is produced under the control of matrix metallo-proteinases, TNF-α, and TGF-β2, factors known to be involved in glaucoma. CXCL12 protects in vitro trabecular cells from apoptotic death via CXCR4 whereas SDF-1(5-67) induces apoptosis through CXCR3 and caspase activation. Ocular administration of SDF-1(5-67) in the rat increases intraocular pressure. In contrast, administration of a selective CXCR3 antagonist in a rat model of ocular hypertension decreases intraocular pressure, prevents retinal neurodegeneration, and preserves visual function. The protective effect of CXCR3 antagonism is related to restoration of the trabecular function. These data demonstrate that proteolytic cleavage of CXCL12 is involved in trabecular pathophysiology, and that local administration of a selective CXCR3 antagonist may be a beneficial therapeutic strategy for treating ocular hypertension and subsequent retinal degeneration.

Published
2012
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