1. Functional characterization of NRAMP3 and NRAMP4 from the metal hyperaccumulator Thlaspi caerulescens
- Author
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Pierre Richaud, Ronald Oomen, Hélène Barbier-Brygoo, Jian Wu, Sébastien Thomine, Françoise Lelièvre, Mark G. M. Aarts, Sandrine Blanchet, Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratory of Genetics, Wageningen University and Research [Wageningen] (WUR), Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS), Bioénergie et Microalgues (EBM), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Biologie végétale et microbiologie environnementale - UMR7265 (BVME), UMR Biolologie Végétale & Microbiologie Environementale, Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research Centre [Wageningen] (WUR), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut des sciences du végétal ( ISV ), Centre National de la Recherche Scientifique ( CNRS ), Biochimie et Physiologie Moléculaire des Plantes ( BPMP ), Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Wageningen University and Research Centre [Wageningen] ( WUR ), Institute of Vegetables and Flowers ( IVF ), Chinese Academy of Agricultural Sciences ( CAAS ), Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues ( L3BM ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Biologie végétale et microbiologie environnementale - UMR7265 ( BVME ), and Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Aix Marseille Université ( AMU )
- Subjects
0106 biological sciences ,iron transport ,Mutant ,Arabidopsis ,Plant Science ,MESH : Green Fluorescent Proteins ,01 natural sciences ,Laboratorium voor Plantenveredeling ,Arabidopsis thaliana ,MESH : Cadmium ,transporter genes ,0303 health sciences ,food and beverages ,NRAMP ,Zinc ,Laboratory of Genetics ,MESH : Vacuoles ,MESH : Cloning, Molecular ,zinc transporter ,Recombinant Fusion Proteins ,Molecular Sequence Data ,Saccharomyces cerevisiae ,Laboratorium voor Erfelijkheidsleer ,MESH: Vacuoles ,03 medical and health sciences ,MESH: Green Fluorescent Proteins ,Thlaspi caerulescens ,Botany ,MESH: Recombinant Fusion Proteins ,MESH: Cloning, Molecular ,MESH: DNA, Plant ,MESH: Metals ,MESH: Molecular Sequence Data ,MESH : Metals ,fungi ,Plant Breeding ,Mutation ,MESH : Gene Expression Regulation, Plant ,MESH: Genome, Plant ,[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology ,MESH : Molecular Sequence Data ,MESH : DNA, Complementary ,Physiology ,MESH : Saccharomyces cerevisiae ,MESH : Thlaspi ,medicine.disease_cause ,MESH: Zinc ,expression differences ,MESH : Arabidopsis ,Gene Expression Regulation, Plant ,MESH: Arabidopsis ,Cloning, Molecular ,Plant Proteins ,metal transport ,zinc (Zn) ,MESH: Genetic Complementation Test ,biology ,MESH: Plant Proteins ,EPS-3 ,MESH: Saccharomyces cerevisiae ,MESH : Genome, Plant ,MESH: Intracellular Membranes ,MESH : DNA, Plant ,Biochemistry ,Metals ,saccharomyces-cerevisiae ,MESH : Mutation ,hyperaccumulator ,Genome, Plant ,DNA, Complementary ,MESH: Mutation ,MESH : Recombinant Fusion Proteins ,DNA, Plant ,cadmium ,heavy-metal ,MESH: Biological Transport ,Green Fluorescent Proteins ,MESH: Cadmium ,MESH: Thlaspi ,arabidopsis-thaliana ,MESH : Plant Proteins ,medicine ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Hyperaccumulator ,MESH: Gene Expression Regulation, Plant ,030304 developmental biology ,MESH : Genetic Complementation Test ,cadmium (Cd) ,ACL ,Genetic Complementation Test ,Biological Transport ,MESH : Zinc ,Intracellular Membranes ,MESH: DNA, Complementary ,biology.organism_classification ,Yeast ,Thlaspi ,MESH : Biological Transport ,MESH : Intracellular Membranes ,Vacuoles ,Zinc toxicity ,gene encodes ,Heterologous expression ,protein ,010606 plant biology & botany - Abstract
International audience; The ability of metal hyperaccumulating plants to tolerate and accumulate heavy metals results from adaptations of metal homeostasis. NRAMP metal transporters were found to be highly expressed in some hyperaccumulating plant species. Here, we identified TcNRAMP3 and TcNRAMP4, the closest homologues to AtNRAMP3 and AtNRAMP4 in Thlaspi caerulescens and characterized them by expression analysis, confocal imaging and heterologous expression in yeast and Arabidopsis thaliana. TcNRAMP3 and TcNRAMP4 are expressed at higher levels than their A. thaliana homologues. When expressed in yeast TcNRAMP3 and TcNRAMP4 transport the same metals as their respective A. thaliana orthologues: iron (Fe), manganese (Mn) and cadmium (Cd) but not zinc (Zn) for NRAMP3; Fe, Mn, Cd and Zn for NRAMP4. They also localize at the vacuolar membrane in A. thaliana protoplasts. Inactivation of AtNRAMP3 and AtNRAMP4 in A. thaliana results in strong Cd and Zn hypersensitivity, which is fully rescued by TcNRAMP3 or TcNRAMP4 expression. However, metal tolerance conferred by TcNRAMP expression in nramp3nramp4 mutant does not exceed that of wild-type A. thaliana. Our data indicate that the difference between TcNRAMP3 and TcNRAMP4 and their A. thaliana orthologues does not lie in a different protein function, but probably resides in a different expression level or expression pattern.
- Published
- 2009
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