1. Nanoparticles for intravascular applications : physicochemical characterization and cytotoxicity testing
- Author
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Rudolf Urbanics, Maya Juenet, Jan Zaloga, Janos Szebeni, László Dézsi, Isabelle Texier, Ruth Prassl, Jens Baumgartner, Christoph Alexiou, Fabrice Navarro, Didier Letourneur, Harald Mangge, Danielle Franke, Damien Faivre, Iwona Cicha, Josbert M. Metselaar, Acarilia Eduardo da Silva, Jasmin Matuszak, Cédric Chauvierre, Gunter Almer, Biomaterials Science and Technology, Faculty of Science and Technology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Department of Biomaterials [Potsdam], Max Planck Institute of Colloids and Interfaces, Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Medical University Graz, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), RWTH University Clinic, and Semmelweis University [Budapest, Hungary]
- Subjects
Male ,0301 basic medicine ,Materials science ,Cell Survival ,Polymers ,Swine ,Dispersity ,Biomedical Engineering ,Medicine (miscellaneous) ,Nanoparticle ,Bioengineering ,Cell analysis ,Nanotechnology ,02 engineering and technology ,Development ,Ferric Compounds ,03 medical and health sciences ,chemistry.chemical_compound ,Cytotoxicity testing ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,General Materials Science ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,ComputingMilieux_MISCELLANEOUS ,Liposome ,Endothelial Cells ,IR-103925 ,021001 nanoscience & nanotechnology ,Endothelial stem cell ,030104 developmental biology ,chemistry ,Cell culture ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,METIS-321012 ,Liposomes ,Nanoparticles ,0210 nano-technology ,Iron oxide nanoparticles ,Biomedical engineering - Abstract
Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.
- Published
- 2016
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