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5 results on '"MTR, methionine synthase"'

1. Gene interactions observed with the HDL-c blood lipid, intakes of protein, sugar and biotin in relation to circulating homocysteine concentrations in a group of black South Africans

Author

Jacomina P. du Plessis, Lizelle Zandberg, Cornelie Nienaber-Rousseau, Alida Melse-Boonstra, 12257656 - Zandberg, Lizelle, 12632449 - Nienaber-Rousseau, Cornelie, 23446307 - Du Plessis, Jacomina P., and 20338457 - Melse-Boonstra, Alida

Subjects
d, Cohen's d-value, Homocysteine, RFLP, restriction fragment length polymorphism, G, guanine, Blood lipids, LDL-c, low density lipoprotein cholesterol, WT, wild type, chemistry.chemical_compound, HDL-c, high-density lipoprotein cholesterol, 0302 clinical medicine, Endocrinology, High-density lipoprotein, PCR, polymerase chain reaction, HW, Hardy Weinberg, ID, identity, THUSA, Transition and Health during Urbanization in South Africa, Methionine synthase, lcsh:QH301-705.5, Nutrigenetics, ins, insertion, 0303 health sciences, lcsh:R5-920, Blood lipid-gene interactions, %TE, percentage of total energy, biology, Nutrient-gene interactions, Gly, glycine, A, adenine, 030305 genetics & heredity, PA, physical activity, MT, mutant type, Val, valine, Gene–diet interactions, PURE, Prospective Urban and Rural Epidemiology, QFFQ, quantitative food frequency questionnaire, SNP, single-nucleotide polymorphism, lcsh:Medicine (General), MTHFR, methylenetetrahydrofolate reductase, Research Paper, GGT, gamma glutamyl transferase, medicine.medical_specialty, Hyperhomocysteinemia, CBS, cystathionine β synthase, Gene-diet interactions, Thr, threonine, Biotin, ISAK, International Society for the Advancement of Kinanthropometry, Total homocysteine, Blood lipid–gene interactions, Asp, aspartic acid, MAF, minor allele frequency, SD, standard deviations, LD, pairwise linkage-disequilibrium, MTR, methionine synthase, Ala, alanine, 03 medical and health sciences, Internal medicine, Genetics, medicine, Sugar, Molecular Biology, Ile, isoleucine, VLAG, Global Nutrition, Wereldvoeding, %TCHO, percentage total carbohydrate intake, SE, standard error, business.industry, C, cytosine, CV, coefficient variation, HWE, Hardy–Weinberg equilibrium, Protein, ES, effect size, Precision nutrition, Hcy, homocysteine, medicine.disease, HHcy, hyperhomocysteinemia, MRC, Medical Research Council, Cystathionine beta synthase, GLM, generalized linear model, Minor allele frequency, bp, base pairs, chemistry, lcsh:Biology (General), CI, confidence intervals, SFA, saturated fatty acids, Nutrient–gene interactions, Methylenetetrahydrofolate reductase, biology.protein, HbA1c, glycated hemoglobin, business, T, thymine, 030217 neurology & neurosurgery
Abstract

Background: Elevated homocysteine (Hcy) is associated with several pathologies. Gene–diet interactions related to Hcy might be used to customize dietary advice to reduce disease incidence. To explore this possibility, we investigated interactions between anthropometry, biochemical markers and diet and single-nucleotide polymorphisms (SNPs) in relation to Hcy concentrations. Five SNPs of Hcy-metabolizing enzymes were analyzed in 2010 black South Africans. Results: Hcy was higher with each additional methylenetetrahydrofolate reductase (MTHFR) C677T minor allele copy, but was lower in methionine synthase (MTR) 2756AA homozygotes than heterozygotes. Individuals harboring cystathionine β synthase (CBS) 833 T/844ins68 had lower Hcy concentrations than others. No interactive effects were observed with any of the anthropometrical markers. MTHFR C677T and CBS T833C/844ins68 homozygote minor allele carriers presented with lower Hcy as high density lipoprotein cholesterol (HDL-c) increased. Hcy concentrations were negatively associated with dietary protein and animal protein intake in the TT and TC genotypes, but positively in the CC genotype of CBS T833C/844ins68. Hcy was markedly higher in TT homozygotes of MTHFR C677T as added sugar intake increased. In CBS T833C/844ins68 major allele carriers, biotin intake was negatively associated with Hcy; but positively in those harboring the homozygous minor allele. Conclusions: The Hcy–SNP associations are modulated by diet and open up the possibility of invoking dietary interventions to treat hyperhomocysteinemia. Future intervention trials should further explore the observed gene–diet and gene–blood lipid interactions. Keywords: Biotin, Blood lipid–gene interactions, Gene–diet interactions, Hyperhomocysteinemia, Nutrient–gene interactions, Nutrigenetics, Precision nutrition, Protein, Sugar, Total homocysteine

Published
2020

2. Gene interactions observed with the HDL-c blood lipid, intakes of protein, sugar and biotin in relation to circulating homocysteine concentrations in a group of black South Africans.

Author

du Plessis JP, Melse-Boonstra A, Zandberg L, and Nienaber-Rousseau C

Abstract

Background: Elevated homocysteine (Hcy) is associated with several pathologies. Gene-diet interactions related to Hcy might be used to customize dietary advice to reduce disease incidence. To explore this possibility, we investigated interactions between anthropometry, biochemical markers and diet and single-nucleotide polymorphisms (SNPs) in relation to Hcy concentrations. Five SNPs of Hcy-metabolizing enzymes were analyzed in 2010 black South Africans., Results: Hcy was higher with each additional methylenetetrahydrofolate reductase ( MTHFR ) C677T minor allele copy, but was lower in methionine synthase ( MTR ) 2756AA homozygotes than heterozygotes. Individuals harboring cystathionine β synthase ( CBS ) 833 T/844ins68 had lower Hcy concentrations than others. No interactive effects were observed with any of the anthropometrical markers. MTHFR C677T and CBS T833C/844ins68 homozygote minor allele carriers presented with lower Hcy as high density lipoprotein cholesterol (HDL-c) increased. Hcy concentrations were negatively associated with dietary protein and animal protein intake in the TT and TC genotypes, but positively in the CC genotype of CBS T833C/844ins68. Hcy was markedly higher in TT homozygotes of MTHFR C677T as added sugar intake increased. In CBS T833C/844ins68 major allele carriers, biotin intake was negatively associated with Hcy; but positively in those harboring the homozygous minor allele., Conclusions: The Hcy-SNP associations are modulated by diet and open up the possibility of invoking dietary interventions to treat hyperhomocysteinemia. Future intervention trials should further explore the observed gene-diet and gene-blood lipid interactions., Competing Interests: The authors declare that they have no competing interests., (© 2019 The Authors.)

Published
2019
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3. Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels

Author

Christian Scherling, Rima Obeid, Jürgen Geisel, Hannelore Daniel, Stefanie Worsch, Bernhard L. Bader, Manuela Sailer, Jarlei Fiamoncini, Christoph Dahlhoff, Björn Hummel, and Kirsten Uebel

Subjects
Hepatic steatosis, ACC, acetyl-CoA carboxylase, HFMS, methyl-donor supplemented high-fat diet, 3-HB, β-hydroxybutyrate, LDL, low density lipoprotein, PC, phosphatidylcholine, Cbs, cystathionine β-synthase, HF, high-fat diet, AMP-activated protein kinase, ANT, adenine nucleotide translocase, Beta oxidation, chemistry.chemical_classification, biology, C1, one-carbon, Fatty liver, VAT, visceral adipose tissue, SAM, S-adenosylmethionine, One-carbon metabolism, TG, triacylglycerol, ddc, Fatty acid synthase, Pemt, phosphatidylethanolamine methyltransferase, Original Article, β-HAD, β-hydroxyacyl CoA dehydrogenase, medicine.drug, MDS, methyl-donor supplementation, NAFLD, non-alcoholic fatty liver disease, medicine.medical_specialty, GNMT, glycine N-methyltransferase, C, control diet, Fasn, fatty acid synthase, HSP90, heat shock protein 90, Gapdh, glyceraldehyde 3-phosphate dehydrogenase, MTR, methionine synthase, VLDL, very low density lipoprotein, Internal medicine, NEFA, non-esterified fatty acids, medicine, Obesity, Carnitine, SREBP1c, sterol regulatory element-binding protein-1c, Bhmt, betaine-homocysteine methyltransferase, Molecular Biology, Acyl-carnitines, Cpt1a, carnitine palmitoyltransferase-1a, SM, sphingomyelin, CACT, carnitine-acylcarnitine transporter, SAH, S-adenosylhomocysteine, Fatty acid, AMPK, DIO, diet-induced obesity, PL, phospholipids, Cell Biology, Hcy, homocysteine, medicine.disease, AMPK, AMP-activated protein kinase, Endocrinology, PPARα, peroxisome proliferator-activated receptor-α, chemistry, MAT, methionine adenosyltransferase, HMW adiponectin, high molecular weight adiponectin, Hprt1, hypoxanthine phosphoribosyltransferase 1, MCD, malonyl-CoA decarboxylase, biology.protein, β-oxidation, PGC1α, peroxisome proliferator-activated receptor-γ co-activator-1α, CMS, methyl-donor supplemented control diet, Steatosis
Abstract

Non-alcoholic fatty liver disease (NAFLD) results from increased hepatic lipid accumulation and steatosis, and is closely linked to liver one-carbon (C1) metabolism. We assessed in C57BL6/N mice whether NAFLD induced by a high-fat (HF) diet over 8 weeks can be reversed by additional 4 weeks of a dietary methyl-donor supplementation (MDS). MDS in the obese mice failed to reverse NAFLD, but prevented the progression of hepatic steatosis associated with major changes in key hepatic C1-metabolites, e.g. S-adenosyl-methionine and S-adenosyl-homocysteine. Increased phosphorylation of AMPK-α together with enhanced β-HAD activity suggested an increased flux through fatty acid oxidation pathways. This was supported by concomitantly decreased hepatic free fatty acid and acyl-carnitines levels. Although HF diet changed the hepatic phospholipid pattern, MDS did not. Our findings suggest that dietary methyl-donors activate AMPK, a key enzyme in fatty acid β-oxidation control, that mediates increased fatty acid utilization and thereby prevents further hepatic lipid accumulation.

Published
2014

4. Folate-related genes and the risk of tobacco-related cancers in Central Europe

Author

Rayjean J. Hung, Ioan Nicolae Mates, Neonila Szeszenia-Dabrowska, Jolanta Lissowska, Lenka Foretova, Mia Hashibe, David Zaridze, Valerie Gaborieau, Peter Rudnai, Federico Canzian, Vladimir Janout, Vladimir Bencko, Janet Hall, James McKay, Paolo Boffetta, Norman Moullan, Paul Brennan, Eleonora Fabianova, Amelie Chabrier, Hung, R.J., Hashibe, M., McKay, J., Gaborieau, V., Szeszenia-Dabrowska, N., Zaridze, D., Lissowska, J., Rudnai, P., Fabianova, E., Mates, I., Foretova, L., Janout, V., Bencko, V., Chabrier, A., Moullan, N., Canzian, F., Hall, J., Boffetta, P., and Brennan, P.

Subjects
Oncology, Cancer Research, Linkage disequilibrium, medicine.medical_specialty, Pathology, 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase, Polymorphism, Single Nucleotide, MTR, methionine synthase, Folic Acid, Risk Factors, Internal medicine, Neoplasms, Genotype, Tobacco, medicine, Humans, Genetic Predisposition to Disease, Risk factor, Lung cancer, OR, odds ratio, Methylenetetrahydrofolate Reductase (NADPH2), biology, business.industry, Confounding, Cancer, General Medicine, Odds ratio, medicine.disease, CI, confidence interval, Europe, Methylenetetrahydrofolate reductase, Case-Control Studies, biology.protein, business, MTHFR, methylenetetrahydrofolate reductase
Abstract

Folate has been hypothesized to protect against aero-digestive cancers although the evidence is not yet conclusive due to possible confounding by other dietary factors. Sequence variants in folate pathway were suggested to be associated with plasma folate levels and are unlikely to be confounded by other lifestyle factors. We therefore investigated the effects of key folate genetic variants on the risk of aero-digestive cancers and their potential effect modification by folate intake in a multicenter study in Central Europe. A total of 2250 lung cases, 811 upper aero-digestive tract cases and 2899 controls were recruited with blood samples. The methylenetetrahydrofolate reductase (MTHFR) C677T variant was associated with an increased risk of lung cancer with an odds ratio (OR) for homozygote variant of 1.37 [95% confidence interval (CI) =1.10-;1.71]. The two MTHFR variants were in strong linkage disequilibrium, and 677T-1298A appeared to be the primary haplotype associated with cancer risk. The risk estimates for MTHFR 677T/677T genotype was more prominent among lung cancer patients with young onset (OR=1.92,95% CI =1.12-3.29). When stratified by dietary intake of folate, the effect of the MTHFR 677T variant was more prominent among subjects with low intake of folate: the ORs for 677T/677T genotype among subjects with the lowest decile were 2.60 (95% CI =1.39-4.88) and 4.14 (95% CI =1.47-11.7) for lung and upper aero-digestive tract cancer, respectively. In conclusion, we identified a moderate effect of MTHFR C677T on lung cancer risk and a possible effect modification by folate intake that is consistent with the functional data. These results support an important role of folate in protecting against tobacco-related cancers. © The Author 2007. Published by Oxford University Press. All rights reserved.

Published
2007

5. Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels.

Author

Dahlhoff C, Worsch S, Sailer M, Hummel BA, Fiamoncini J, Uebel K, Obeid R, Scherling C, Geisel J, Bader BL, and Daniel H

Abstract

Non-alcoholic fatty liver disease (NAFLD) results from increased hepatic lipid accumulation and steatosis, and is closely linked to liver one-carbon (C1) metabolism. We assessed in C57BL6/N mice whether NAFLD induced by a high-fat (HF) diet over 8 weeks can be reversed by additional 4 weeks of a dietary methyl-donor supplementation (MDS). MDS in the obese mice failed to reverse NAFLD, but prevented the progression of hepatic steatosis associated with major changes in key hepatic C1-metabolites, e.g. S-adenosyl-methionine and S-adenosyl-homocysteine. Increased phosphorylation of AMPK-α together with enhanced β-HAD activity suggested an increased flux through fatty acid oxidation pathways. This was supported by concomitantly decreased hepatic free fatty acid and acyl-carnitines levels. Although HF diet changed the hepatic phospholipid pattern, MDS did not. Our findings suggest that dietary methyl-donors activate AMPK, a key enzyme in fatty acid β-oxidation control, that mediates increased fatty acid utilization and thereby prevents further hepatic lipid accumulation.

Published
2014
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