1. Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
- Author
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Jialin Jia, Ping Liu, Xiaohui Zhu, Jing Wang, Ma Jieliang, Yaxin Yao, Jin Huang, and Sijia Lu
- Subjects
General Chemical Engineering ,medicine.medical_treatment ,Fertilization in Vitro ,Biology ,Chromosomes ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Andrology ,Pregnancy ,medicine ,Humans ,Inner cell mass ,Genetic Testing ,Preimplantation Diagnosis ,reproductive and urinary physiology ,Whole Genome Amplification ,Ploidies ,In vitro fertilisation ,General Immunology and Microbiology ,General Neuroscience ,Chromosome ,Embryo culture ,Embryo ,Aneuploidy ,Blastocyst ,embryonic structures ,Female ,Sample collection - Abstract
In clinical in vitro fertilization (IVF), the prevailing method for PGT-A requires biopsy of a few cells from the trophectoderm (TE). This is the lineage that forms the placenta. This method, however, requires specialized skills, is invasive, and suffers from false positives and negatives because the chromosome numbers in the TE and the inner cell mass (ICM), which develops into the fetus, are not always the same. NICS, a technology requiring sequencing of DNA that released into the culture medium from both TE and ICM, may offer a way out to these problems but has previously been shown to have limited efficacy. The present study reports the full protocol of NICS, which includes culture medium sampling methods, whole genome amplification (WGA) and library preparation, and NGS data analysis by analysis software. Considering the different cryopreservation times in different embryo laboratories, embryologists have two methods for collecting embryo culture medium that can be selected according to the actual conditions of the IVF laboratory.
- Published
- 2021