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23 results on '"Ma, Xingkai"'

17. The NRF2/KEAP1 Pathway Modulates Nasopharyngeal Carcinoma Cell Radiosensitivity via ROS Elimination

Author

Zhou, Jieyu, Ding, Jiping, Ma, Xingkai, Zhang, Meichao, Huo, Zirong, Yao, Yuan, Li, Dong, and Wang, Zhentao

Subjects
KEAP1, stomatognathic diseases, radiosensitivity, nasopharyngeal carcinoma, otorhinolaryngologic diseases, respiratory system, environment and public health, OncoTargets and Therapy, Original Research, NRF2
Abstract

Jieyu Zhou,1,* Jiping Ding,2,* Xingkai Ma,3,* Meichao Zhang,2 Zirong Huo,1 Yuan Yao,2 Dong Li,2 Zhentao Wang1 1Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China; Ear Institute, Shanghai Jiaotong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, People’s Republic of China; 2Department of Radiation Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Otolaryngology, Zhangjiagang First People’s Hospital, Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dong Li; Zhentao Wang Email lidong@shsmu.edu.cn; 13916548333@163.comPurpose: Radioresistance is a vital obstacle for the prognosis of human nasopharyngeal carcinoma (NPC), but the underlying mechanism is still unknown. Here, we explored the role of the NRF2/KEAP1 pathway in radioresistance of NPC cell lines.Materials and Methods: We selected NPC cell lines CNE-1 and CNE-2, treated them with ionization, and subsequently determined the levels of NRF2, KEAP1, antioxidant enzymes, and ROS. We then evaluated the effect of NRF2 or KEAP1 inhibition on cell proliferation, colony formation, and radiosensitivity in CNE2 cells.Results: We discovered that the NRF2/KEAP1 signaling pathway can be activated by radiotherapy in NPC cells, while NRF2 knockdown enhances the sensitivity of CNE-2 cells to radiation treatment. In contrast, the silencing of KEAP1 inhibits the sensitivity of CNE-2 cells to radiation treatment.Conclusion: Our results suggest that NRF2/KEAP1 signaling may serve as an essential regulator of the radioresistance of NPC and may be applied as a novel therapeutic approach for the sensitization of NPC to radiation.Keywords: nasopharyngeal carcinoma, NRF2, KEAP1, radiosensitivity

Published
2020

22. Long noncoding RNA ANCR promotes migration, invasion, EMT progress and stemness of nasopharyngeal carcinoma cells via the miR-4731-5p/NMT1 axis.

Author

Ma X, Yuan Y, Lu J, Li M, Yu Y, Liu J, and Zhou J

Subjects
Cell Line, Tumor, Cell Proliferation genetics, Epithelial-Mesenchymal Transition genetics, Gene Expression Regulation, Neoplastic, Humans, Nasopharyngeal Neoplasms genetics, Cell Movement genetics, MicroRNAs genetics, Nasopharyngeal Carcinoma pathology, Nasopharyngeal Neoplasms pathology, RNA, Long Noncoding genetics
Abstract

Background: In our previous study, we revealed that Antidifferentiation noncoding RNA (ANCR) promoted proliferation and radiation resistance of nasopharyngeal carcinoma (NPC) cells. However, the molecular mechanism and function of ANCR are not fully studied. The current study aimed to further investigate the role and underlying molecular mechanism of ANCR in NPC., Methods: RT-qPCR and western blot analyses were used to detect the levels of RNAs and proteins in NPC cells. Wound healing and Transwell assays were used to examine the migration and invasion of NPC cells. The relationship among ANCR, miR-4731-5p and N-myristoyltransferase 1 (NMT1) was investigated by RIP and luciferase reporter assays. The NPC cell stemness was accessed by the sphere formation assay., Results: ANCR was significantly highly expressed in NPC cell lines. Silenced ANCR suppressed cell migration, invasion epithelial-mesenchymal transition (EMT) process and cell stemness in NPC. Furthermore, ANCR sponged miR-4731-5p to upregulate the NMT1 expression. Rescue assays indicated that NMT1 neutralized the antioncogenic effect induced by silenced ANCR on NPC cells., Conclusions: Long noncoding RNA ANCR suppresses malignant behaviors of nasopharyngeal carcinoma cells by regulating miR-4731-5p/NMT1 axis., (Copyright © 2021 Elsevier GmbH. All rights reserved.)

Published
2021
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23. [Application of acoustic rhinometry in assessment of preschool children nasal cavity volume].

Author

Shen Y, Zhao L, Ma X, and Qian W

Subjects
Airway Resistance, Child, Preschool, Female, Humans, Male, Reference Values, Nasal Cavity anatomy & histology, Nasal Cavity physiology, Rhinometry, Acoustic methods
Abstract

Objective: Acoustic rhinometry (AR) was performed to standardize the measurement techniques, result interpretation and reference values of nasal cavity volume (NV) in preschool children., Method: (1) Nasal cavity models were used to test the correlations between NV, minimal cross-sectional area (MCA), and nasal resistance. (2) There were 97 four-year-old and 137 five-year-old children underwent AR test., Result: (1) Model tests showed that resist the nce were better correlated with the change of volume than the MCA. (2) The average bilateral NV in preschool children was (2.03 +/- 0.4) ml. No significant gender and age difference were observed (P>0.05)., Conclusion: Volume measurement appears more sensitive and reliable than the MCA in assessing nasal patency. The AR result interpretation and normative NV values in preschool children are introduced.

Published
2008

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