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1. Evaluation of nut traits of dominant varieties of Ping􀆳ou hybrid hazelnut in the north of Anhui Province

Author

WANG Lujun, SUN Jing, ZHOU Misheng, LIANG Lisong, and MA Qinghua

Subjects
ping􀆳ou hybrid hazelnuts, dominant varieties, economic traits, mineral compositions, fatty acids, nutritional substances, Biology (General), QH301-705.5, Botany, QK1-989
Abstract

Abstract [Objective] This study aims to evaluate the nut traits of the dominant varieties of Ping􀆳ou hybrid hazelnuts cultivated in the northern Anhui and analyze the suitability of the varieties for processing, to provide a basis for the selection and popularization of the hazelnuts. [Methods] Nine varieties were selected as the dominant varieties based on their adaptation and yield in the northern Anhui. These varieties were analyzed and evaluated from different processing and utilization perspectives, such as baking, kernel, oil, and fresh-eating. [Results] (1) The distribution ranges of single nut mass, nut size, nut shape index, kernel mass, shell thickness, and kernel mass percentage of 9 dominant varieties were 2.54-4.40 g, 18.99 -21.49 mm, 0.67-1.02, 1.00-1.66 g, 1.53-2.41 mm, and 34.36%-47.01%, respectively. The distribution ranges of fat, protein, and soluble sugar contents were 50.50-58.00 g/(100 g), 21.10- 25.10 g/(100 g), and 4.08%-4.94%, respectively. (2) 8 fatty acids were detected from the hazelnut kernels, among which oleic acid was the highest with a distribution range of 81.21%-88.53%. The content of unsaturated fatty acids was 92.19%-94.61% among the total content of fat in hazelnut kernels. For mineral nutrition, the content of potassium, phosphorus, calcium, and magnesium in hazelnut kernels was relatively rich. (3) As for the processing capacity, the distribution ranges of single plant yield, fat production, and protein production of 9 dominant varieties were 1.52-2.20 kg, 330.50-503.47 g, and 141.68-224.40 g, respectively. [Conclusion] According to different consumption patterns, ‘Liaozhen 3’ is best for baking market, ‘Liaozhen 3’, ‘Dawei’ and ‘Liaozhen 4’ are best for kernel market, and ‘Dawei’, ‘Liaozhen 3’ and ‘Ping􀆳ou 545’ are best for the fresh-eating market. ‘Liaozhen 4’ can be used as oil processing varieties, ‘Liaozhen 1’ as functional protein beverage processing varieties, and ‘Dawei’ and ‘Liaozhen 9’ as high calcium processing varieties.

Published
2024
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2. A method for numerical simulation of mode transition process of compression system of variable cycle engine based on throttle valve model

Author

WANG Hao, LIANG Xiwen, MA Qinghua, and Wang Yangang

Subjects
variable cycle engine, mode transition, throttle model, numerical simulation, Motor vehicles. Aeronautics. Astronautics, TL1-4050
Abstract

In order to study the aerodynamic characteristics of the compression system of a variable-cycle engine during its mode transition, a low-order model was established based on the throttle valve theory to describe the throttle characteristics of the mode selection valve at its different opening degrees. The model was applied to the outlet of a double-bypass variable-cycle fan in the form of characteristic boundary. A 3D low-order hybrid computational model was established for the numerical simulation of flow fields in the mode transition process of the compression system. The calculation accuracy and effectiveness of the throttle valve model were verified by comparing its calculation results with those of the 3D valve model. The simulation method was further applied to the prediction and analysis of the fan performance variation during the single and double bypass mode transitions of the variable-cycle fan. The simulation results show that: the throttle coefficient, which represents the throttle intensity, has something to do only with the flow area ratio of the valve but has nothing to do with its angle. Therefore, the throttle valve model is universal for valves with different motion angles; the throttle valve model can accurately predict the variation trend of the aerodynamic characteristics of the fan in all stages of the mode transition process. Compared with the 3D valve model, the prediction error of the pressure ratio of the fan in all stages is less than 1.93%, and the efficiency error is less than 1.05%. In the valve closing process, the second-stage rotor performance changes more dramatically, and the first-stage rotor performance changes with lag.

Published
2022
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5. Long-term sphere culture cannot maintain a high ratio of cancer stem cells: a mathematical model and experiment.

Author

Tang Peng, Ma Qinghua, Tang Zhenning, Wang Kaifa, and Jiang Jun

Subjects
Medicine, Science
Abstract

Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary differential equations to derive the continuous variation of the CSC ratio in long-term sphere culture and estimated the model parameters based on a long-term sphere culture of MCF-7 stem cells. We found that the CSC ratio in long-term sphere culture presented as gradually decreased drift and might be stable at a lower level. Furthermore, we found that fitted model parameters could explain the main growth pattern of CSCs and differentiated cancer cells in long-term sphere culture.

Published
2011
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6. A revised comparison between FF five-factor model and three-factor model,based on China's A-share market

Author

Zhang, Zhijing, Yu, Yue, Ma, Qinghua, and Yao, Haixiang

Subjects
Quantitative Finance - General Finance, Economics - General Economics
Abstract

In allusion to some contradicting results in existing research, this paper selects China's latest stock data from 2005 to 2020 for empirical analysis. By choosing this periods' data, we avoid the periods of China's significant stock market reforms to reduce the impact of the government's policy on the factor effect. In this paper, the redundant factors (HML, CMA) are orthogonalized, and the regression analysis of 5*5 portfolio of Size-B/M and Size-Inv is carried out with these two orthogonalized factors. It found that the HML and the CMA are still significant in many portfolios, indicating that they have a strong explanatory ability, which is also consistent with the results of GRS test. All these show that the five-factor model has a better ability to explain the excess return rate. In the concrete analysis, this paper uses the methods of the five-factor 25-group portfolio returns calculation, the five-factor regression analysis, the orthogonal treatment, the five-factor 25-group regression and the GRS test to more comprehensively explain the excellent explanatory ability of the five-factor model to the excess return. Then, we analyze the possible reasons for the strong explanatory ability of the HML, CMA and RMW from the aspects of price to book ratio, turnover rate and correlation coefficient. We also give a detailed explanation of the results, and analyze the changes of China's stock market policy and investors' investment style recent years. Finally, this paper attempts to put forward some useful suggestions on the development of asset pricing model and China's stock market., Comment: 17 pages, under review

Published
2021

10. Identification of hypoxic macrophages in glioblastoma with therapeutic potential for vasculature normalization

Author

Wang, Wenying, Li, Tianran, Cheng, Yue, Li, Fei, Qi, Shuhong, Mao, Min, Wu, Jingjing, Liu, Qing, Zhang, Xiaoning, Li, Xuegang, Zhang, Lu, Qi, Haoyue, Yang, Lan, Yang, Kaidi, He, Zhicheng, Ding, Shuaishuai, Qin, Zhongyi, Yang, Ying, Yang, Xi, Luo, Chunhua, Guo, Ying, Wang, Chao, Liu, Xindong, Zhou, Lei, Liu, Yuqi, Kong, Weikai, Miao, Jingya, Ye, Shuanghui, Luo, Min, An, Lele, Wang, Lujing, Che, Linrong, Niu, Qin, Ma, Qinghua, Zhang, Xia, Zhang, Zhihong, Hu, Rong, Feng, Hua, Ping, Yi-Fang, Bian, Xiu-Wu, and Shi, Yu

Published
2024
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14. Research on Precision Distribution Method of the Antiaircraft Weapon Guidance System

Author

Yang, Shujun, Ma, Qinghua, Zheng, Jianqiang, Wang, Shuaiwei, Ma, JiRong, Liang, Yiming, Li, Duansong, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Zhang, Weicun, editor, Fu, Yongling, editor, Yu, Zhiyuan, editor, and Zheng, Song, editor

Published
2022
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15. A Strong and Robust Guidance Handover for a Certain Type of Air Defense Missile Strategy Research

Author

Yang, Shujun, Ma, Qinghua, Zheng, Jianqiang, Wang, Shuaiwei, Ma, JiRong, Li, Duansong, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Zhang, Weicun, editor, Fu, Yongling, editor, Yu, Zhiyuan, editor, and Zheng, Song, editor

Published
2022
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20. Elevated Kir2.1/nuclear N2ICD defines a highly malignant subtype of non-WNT/SHH medulloblastomas

Author

Wang, Yan-Xia, Wu, Haibo, Ren, Yong, Lv, Shengqing, Ji, Chengdong, Xiang, Dongfang, Zhang, Mengsi, Lu, Huimin, Fu, Wenjuan, Liu, Qing, Yan, Zexuan, Ma, Qinghua, Miao, Jingya, Cai, Ruili, Lan, Xi, Wu, Bin, Wang, Wenying, Liu, Yinhua, Wang, Dai-Zhong, Cao, Mianfu, He, Zhicheng, Shi, Yu, Ping, Yifang, Yao, Xiaohong, Zhang, Xia, Zhang, Peng, Wang, Ji Ming, Wang, Yan, Cui, Youhong, and Bian, Xiu-Wu

Published
2022
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22. Improved Adaptive Filter with Unknown Process and Measurement Noise Covariance

Author

Ma, Jirong, Hu, Yumei, Ma, Qinghua, Yang, Shujun, Zheng, Jianqiang, Wang, Shuaiwei, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Zhang, Weicun, editor, and Fu, Yongling, editor

Published
2021
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23. Design of Semi-physical Simulation System for Multi-target Attack Air Defense Missile Weapon System

Author

Yang, Shujun, Zheng, Jianqiang, Ma, Qinghua, Wang, Shuaiwei, Ma, Jirong, Deng, Haipeng, Liang, Yiming, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Zhang, Weicun, editor, and Fu, Yongling, editor

Published
2021
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24. Consistency Transformation Project of Target Information in Air Defense Weapon System

Author

Yang, Shujun, Zheng, Jianqiang, Ma, Qinghua, Wang, Shuaiwei, Liang, Yiming, Deng, Haipeng, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martin, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Du, Junping, editor, and Zhang, Weicun, editor

Published
2020
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25. Research on Self-compensation Technology of Strapdown Inertial Navigation System

Author

Zheng, Jianqiang, Gao, Lin, Ma, Qinghua, Yang, Shujun, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Martin, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Du, Junping, editor, and Zhang, Weicun, editor

Published
2020
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26. Research on Cooperative Operation of Air Combat Based on Multi-agent

Author

Zheng, Jianqiang, Ma, Qinghua, Yang, Shujun, Wang, Shuaiwei, Liang, Yiming, Ma, Jirong, Kacprzyk, Janusz, Series Editor, Pal, Nikhil R., Advisory Editor, Bello Perez, Rafael, Advisory Editor, Corchado, Emilio S., Advisory Editor, Hagras, Hani, Advisory Editor, Kóczy, László T., Advisory Editor, Kreinovich, Vladik, Advisory Editor, Lin, Chin-Teng, Advisory Editor, Lu, Jie, Advisory Editor, Melin, Patricia, Advisory Editor, Nedjah, Nadia, Advisory Editor, Nguyen, Ngoc Thanh, Advisory Editor, Wang, Jun, Advisory Editor, Ahram, Tareq, editor, Taiar, Redha, editor, Gremeaux-Bader, Vincent, editor, and Aminian, Kamiar, editor

Published
2020
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33. Functional Characterization of Abdominal-A in the Pine Caterpillar Moth, Dendrolimus punctatus.

Author

Liu, Huihui, Chen, Lin, Ma, Qinghua, Wang, Xinghong, Zhang, Sufang, and Zhou, Xuguo

Subjects
HOMEOBOX genes, REGULATOR genes, INSECT development, INSECT genes, EMBRYOLOGY
Abstract

Hox genes, specifically the bithorax complex (ubx, abdominal-a, and abdominal-b), play a crucial role in specifying posterior abdominal development and serve as key regulators of germline gene development in insects. However, the function of the bithorax complex in the pine caterpillar moth, Dendrolimus punctatus, a major pine tree defoliator in China, remains largely unknown. Specifically, Abdominal-A (Abd-a) controls regional variation in abdominal segmentation in model insects such as Drosophila and Tribolium; however, its role in D. Punctatus remains unexplored. In this study, CRISPR/Cas9 was used to functionally characterize Abd-a in D. punctatus. Two target sites were selected, and the genotypes and phenotypes of the G0 and G1 generations were evaluated. Our findings indicate that knocking out Abd-a led to an abnormality in the posterior segments A2–A7, as well as the loss of appendages, mainly prolegs, and affected the thoracic T3 segmentation as well as wing development. Moreover, mutation in Abd-a also impacted anal and reproductive development. Taken together, these results demonstrate that DpAbd-a is essential for embryonic and reproductive development in D. punctatus and could be a promising target for genetic control of this devastating conifer defoliator. [ABSTRACT FROM AUTHOR]

Published
2024
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34. A Target Information Conversion Method of Antiaircraft Weapon System

Author

Yang, Shujun, Zheng, Jianqiang, Liang, Yiming, Ma, Qinghua, Wang, Shuaiwei, Deng, Haipeng, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Ruediger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Liang, Qilian, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zhang, Junjie James, Series Editor, Jia, Yingmin, editor, Du, Junping, editor, and Zhang, Weicun, editor

Published
2019
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35. VisDrone-VDT2018: The Vision Meets Drone Video Detection and Tracking Challenge Results

Author

Zhu, Pengfei, Wen, Longyin, Du, Dawei, Bian, Xiao, Ling, Haibin, Hu, Qinghua, Wu, Haotian, Nie, Qinqin, Cheng, Hao, Liu, Chenfeng, Liu, Xiaoyu, Ma, Wenya, Wang, Lianjie, Schumann, Arne, Wang, Dan, Ortego, Diego, Luna, Elena, Michail, Emmanouil, Bochinski, Erik, Ni, Feng, Bunyak, Filiz, Zhang, Gege, Seetharaman, Guna, Li, Guorong, Yu, Hongyang, Kompatsiaris, Ioannis, Zhao, Jianfei, Gao, Jie, Martínez, José M., San Miguel, Juan C., Palaniappan, Kannappan, Avgerinakis, Konstantinos, Sommer, Lars, Lauer, Martin, Liu, Mengkun, Al-Shakarji, Noor M., Acatay, Oliver, Giannakeris, Panagiotis, Zhao, Qijie, Ma, Qinghua, Huang, Qingming, Vrochidis, Stefanos, Sikora, Thomas, Senst, Tobias, Song, Wei, Tian, Wei, Zhang, Wenhua, Zhao, Yanyun, Bai, Yidong, Wu, Yinan, Wang, Yongtao, Li, Yuxuan, Pi, Zhaoliang, Ma, Zhiming, Hutchison, David, Series Editor, Kanade, Takeo, Series Editor, Kittler, Josef, Series Editor, Kleinberg, Jon M., Series Editor, Mattern, Friedemann, Series Editor, Mitchell, John C., Series Editor, Naor, Moni, Series Editor, Pandu Rangan, C., Series Editor, Steffen, Bernhard, Series Editor, Terzopoulos, Demetri, Series Editor, Tygar, Doug, Series Editor, Leal-Taixé, Laura, editor, and Roth, Stefan, editor

Published
2019
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45. FANCD2 deficiency sensitizes SHH medulloblastoma to radiotherapy via ferroptosis.

Author

Zhou, Hong, Wang, Yan‐Xia, Wu, Min, Lan, Xi, Xiang, Dongfang, Cai, Ruili, Ma, Qinghua, Miao, Jingya, Fang, Xuanyu, Wang, Junjie, Luo, Dan, He, Zhicheng, Cui, Youhong, Liang, Ping, Wang, Yan, and Bian, Xiu‐Wu

Subjects
MEDULLOBLASTOMA, GENE expression, FANCONI'S anemia, GLUTATHIONE peroxidase, LACTATE dehydrogenase
Abstract

Radiotherapy is one of the standard therapeutic regimens for medulloblastoma (MB). Tumor cells utilize DNA damage repair (DDR) mechanisms to survive and develop resistance during radiotherapy. It has been found that targeting DDR sensitizes tumor cells to radiotherapy in several types of cancer, but whether and how DDR pathways are involved in the MB radiotherapy response remain to be determined. Single‐cell RNA sequencing was carried out on 38 MB tissues, followed by expression enrichment assays. Fanconi anemia group D2 gene (FANCD2) expression was evaluated in MB samples and public MB databases. The function of FANCD2 in MB cells was examined using cell counting assays (CCK‐8), clone formation, lactate dehydrogenase activity, and in mouse orthotopic models. The FANCD2‐related signaling pathway was investigated using assays of peroxidation, a malondialdehyde assay, a reduced glutathione assay, and using FerroOrange to assess intracellular iron ions (Fe2+). Here, we report that FANCD2 was highly expressed in the malignant sonic hedgehog (SHH) MB subtype (SHH‐MB). FANCD2 played an oncogenic role and predicted worse prognosis in SHH‐MB patients. Moreover, FANCD2 knockdown markedly suppressed viability, mobility, and growth of SHH‐MB cells and sensitized SHH‐MB cells to irradiation. Mechanistically, FANCD2 deficiency led to an accumulation of Fe2+ due to increased divalent metal transporter 1 expression and impaired glutathione peroxidase 4 activity, which further activated ferroptosis and reduced proliferation of SHH‐MB cells. Using an orthotopic mouse model, we observed that radiotherapy combined with silencing FANCD2 significantly inhibited the growth of SHH‐MB cell‐derived tumors in vivo. Our study revealed FANCD2 as a potential therapeutic target in SHH‐MB and silencing FANCD2 could sensitize SHH‐MB cells to radiotherapy via inducing ferroptosis. © 2024 The Pathological Society of Great Britain and Ireland. [ABSTRACT FROM AUTHOR]

Published
2024
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