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Aim Predict empirically the current and recent historical (c1970) landscape connectivity and population size of the African lion as a baseline against which to assess conservation of the species. Location Continental Africa. Methods We compiled historical records of lion distribution to generate a recent historical range for the species. Historical population size was predicted using a generalized additive model. Resistant kernel and factorial least-cost path analyses were used to predict recent historical landscape connectivity and compare this with contemporary connectivity at continental, regional and country scales. Results We estimate a baseline population of ~92,054 (83,017–101,094 95% CI) lions in c1970, suggesting Africa's lion population has declined by ~75%, over the last five decades. Although greatly reduced from historical extents (c1500AD), recent historical lion habitat was substantially connected. However, in comparison, contemporary population connectivity has declined dramatically, with many populations now isolated, as well as large declines within remaining population core areas. This decline was most marked in the West and Central region, with a 90% decline in connected habitat compared with its c1970 extent. The Eastern and Southern regions have experienced lower, though significant, declines in connected habitat (44% and 55%, respectively). Contemporary populations are connected by three non-core habitat linkages and 15 potential corridors (spanning unconnected habitat) that may allow dispersal and gene flow. Declining connectivity mirrors recent studies showing loss of genetic diversity and increasing genetic isolation of lion populations. Main conclusions We provide an empirically derived baseline for African lion population size, habitat extent and connectivity in c1970 and at present against which to evaluate contemporary conservation of the species, avoiding a shifting baseline syndrome where conservation success/failure is measured only against recent population size or range. We recommend priorities for conservation of existing connections to avoid further fragmentation.

Species Distribution Models (SDMs) are a powerful tool to derive habitat suitability predictions relating species occurrence data with habitat features. Two of the most frequently applied algorithms to model species-habitat relationships are Generalised Linear Models (GLM) and Random Forest (RF). The former is a parametric regression model providing functional models with direct interpretability. The latter is a machine learning non-parametric algorithm, more tolerant than other approaches in its assumptions, which has often been shown to outperform parametric algorithms. Other approaches have been developed to produce robust SDMs, like training data bootstrapping and spatial scale optimisation. Using felid presence-absence data from three study regions in Southeast Asia (mainland, Borneo and Sumatra), we tested the performances of SDMs by implementing four modelling frameworks: GLM and RF with bootstrapped and non-bootstrapped training data. With Mantel and ANOVA tests we explored how the four combinations of algorithms and bootstrapping influenced SDMs and their predictive performances. Additionally, we tested how scale-optimisation responded to species' size, taxonomic associations (species and genus), study area and algorithm. We found that choice of algorithm had strong effect in determining the differences between SDMs' spatial predictions, while bootstrapping had no effect. Additionally, algorithm followed by study area and species, were the main factors driving differences in the spatial scales identified. SDMs trained with GLM showed higher predictive performance, however, ANOVA tests revealed that algorithm had significant effect only in explaining the variance observed in sensitivity and specificity and, when interacting with bootstrapping, in Percent Correctly Classified (PCC). Bootstrapping significantly explained the variance in specificity, PCC and True Skills Statistics (TSS). Our results suggest that there are systematic differences in the scales identified and in the predictions produced by GLM vs. RF, but that neither approach was consistently better than the other. The divergent predictions and inconsistent predictive abilities suggest that analysts should not assume machine learning is inherently superior and should test multiple methods. Our results have strong implications for SDM development, revealing the inconsistencies introduced by the choice of algorithm on scale optimisation, with GLM selecting broader scales than RF.

As more ambitious protected area (PA) targets for the post-2020 global biodiversity framework are set beyond Aichi Target 11, renew thinking into spatial prioritisation is required to enable PA expansion that maximises environmental values. Our study focuses on the biodiverse and forest-rich Indonesian island of Sulawesi, which has a terrestrial PA network that covers 10% of the island. We used Marxan to investigate trade-offs in the design of an expanded PA network that prioritised different conservation features (biodiversity, forest cover, carbon stock, karst and valuable metal-rich areas) under varying island-wide coverage targets (17%, 30%, and 50%). Our first scenario, which required existing PAs to be selected, required larger areas to meet these coverage targets, in contrast to our second scenario, which allowed for any part of the island to be chosen, irrespective of PA status. The vast Mekongga and Bangkiriang Landscapes, and Gorontalo corridor were consistently identified as a high priority for protection under all scenarios. To meet our conservation targets through expanding current PAs, creating new PAs, and creating corridors that connect existing PAs, we used a spatially explicit three-phase approach. Our findings identified 26,508 km2 of priority areas to be included in the current PA network, potentially assisting Indonesia in meeting its post-2020 GBF target, if our approach is replicated across Indonesia as a national or sub-national analysis. We discuss various land management options through other effective area-based conservation measures (OECMs) and the costs to deliver this strategy.

Herpesviruses are ubiquitous pathogens infecting most animals. Although host immunity continually coevolves to combat virulence, viral variants with enhanced transmissibility or virulence occasionally emerge, resulting in disease burdens in host populations. Mustelid gammaherpesvirus 1 (MusGHV-1) is the only herpesvirus species identified thus far in European badgers, Meles meles. No MusGHV-1 associated pathomorbidity has been reported, but reactivation of MusGHV-1 in genital tracts is linked to impaired female reproductive success. An analysis of a short sequence from the highly conserved DNA polymerase (DNApol) gene previously identified two variants in a single host population. Here we compared genetic variance in blood samples from 66 known individuals of this same free-ranging badger population using a partial sequence comprising 2874 nucleotides of the DNApol gene, among which we identified 15 nucleotide differences resulting in 5 amino acid differences. Prevalence was 86% (59/66) for the common and 17% (11/66) for the novel variant, with 6% (4/66) of badgers presenting with coinfection. MusGHV-1 variants were distributed unevenly across the population, with individuals infected with the novel genotype clustered in 3 of 25 contiguous social groups. Individuals infected with the novel variant had significantly higher MusGHV-1 viral loads in their blood (p = 0.002) after adjusting for age (juveniles > adults, p < 0.001) and season (summer > spring and autumn, p = 0.005; mixed-effect linear regression), likely indicating higher virulence of the novel variant. Further genome-wide analyses of MusGHV-1 host resistance genes and host phenotypic variations are required to clarify the drivers and sequelae of this new MusGHV-1 variant.

The leopard cat (Prionailurus bengalensis) is the most common wild felid in Southeast Asia, yet little is known about the factors that affect their population density and occupancy in natural habitats. Although leopard cats are highly adaptable and reportedly can attain high densities in human-modified habitats, it is not clear which natural habitat is optimal for the species. Also, this felid has been preyed upon by large carnivores in Southeast Asia, yet the intra-guild effects of large carnivore presence on leopard cats are almost unknown. To shed light on these fundamental questions, we used data from camera trap surveys for felids to determine the leopard cat densities in three different forest types within Cambodia: continuous evergreen, mosaic dominated by evergreen (hereafter evergreen mosaic), and mosaic dominated by open dry deciduous forests (hereafter DDF mosaic). We also conducted occupancy analyses to evaluate the interactions of the leopard cats with three large carnivores: leopards (Panthera pardus), dholes (Cuon alpinus), and domestic dogs (Canis familiaris). The estimated density (individuals/100 km2 ± SE) was highest in the continuous evergreen (27.83 ± 7.68), followed by evergreen mosaic (22.06 ± 5.35) and DDF mosaic (13.53 ± 3.23). Densities in all three forest types were relatively high compared to previous studies. Domestic dogs were detected on all 3 sites, and leopards and dholes had sufficient records on only one site each. The occupancy probability of leopard cats was not affected by the presence or absence of any large carnivore, indicating that large carnivores and leopard cats occurred independently of each other. Our findings support the claim that leopard cats are habitat generalists, but we show that evergreen forest is the optimum natural habitat for this species in the region. The DDF mosaic appears to sustain lower densities of leopard cats, probably due to the harsh dry season and wildfires that led to reduced prey base, although this generalist felid was still able to occupy DDF in relatively moderate numbers. Overall, the adaptability of leopard cats to various forest types, and lack of negative interaction with large carnivores, helps to explain why this species is the most common and widespread felid in Southeast Asia.

Maternal immune and/or metabolic conditions relating to stress or nutritional status can affect in utero development among offspring with subsequent implications for later-life responses to infections. We used free-ranging European badgers as a host-pathogen model to investigate how prenatal weather conditions affect later-life herpesvirus genital tract reactivation. We applied a sliding window analysis of weather conditions to 164 samples collected in 2018 from 95 individuals born between 2005–2016. We test if the monthly mean and variation in rainfall and temperature experienced by their mother during the 12 months of delayed implantation and gestation prior to parturition subsequently affected individual herpes reactivation rates among these offspring. We identified four influential prenatal seasonal weather windows that corresponded with previously identified critical climatic conditions affecting badger survival, fecundity and body condition. These all occurred during the pre-implantation rather than the post-implantation period. We conclude that environmental cues during the in utero period of delayed implantation may result in changes that affect an individual's developmental programming against infection or viral reactivation later in life. This illustrates how prenatal adversity caused by environmental factors, such as climate change, can impact wildlife health and population dynamics—an interaction largely overlooked in wildlife management and conservation programmes.

Africa is home to some of the world’s most functionally diverse guilds of large carnivores. However, they are increasingly under threat from anthropogenic pressures that may exacerbate already intense intra-guild competition. Understanding the coexistence mechanisms employed by these species in human-impacted landscapes could help shed light on some of the more subtle ways in which humans may impact wildlife populations, and inform multi-species conservation planning. We used camera trap data from Tanzania’s Ruaha-Rungwa landscape to explore temporal and spatiotemporal associations between members of an intact East African large carnivore guild, and determine how these varied across gradients of anthropogenic impact and protection. All large carnivores except African wild dog (Lycaon pictus) exhibited predominantly nocturnal road-travel behaviour. Leopard (Panthera pardus) appeared to employ minor temporal avoidance of lion (Panthera leo) in all sites except those where human impacts were highest, suggesting that leopard may have been freed up from avoidance of lion in areas where the dominant competitor was less abundant, or that the need for leopard to avoid humans outweighed the need to avoid sympatric competitors. Lion appeared to modify their activity patterns to avoid humans in the most impacted areas. We also found evidence of avoidance and attraction among large carnivores: lion and spotted hyaena (Crocuta crocuta) followed leopard; leopard avoided lion; spotted hyaena followed lion; and lion avoided spotted hyaena. Our findings suggest that large carnivores in Ruaha-Rungwa employ fine-scale partitioning mechanisms to facilitate coexistence with both sympatric species and humans, and that growing human pressures may interfere with these behaviours.

We present a haplotype resolved, diploid genome assembly from a male Meles meles (European badger; Chordata; Mammalia; Carnivora; Mustelidae) using the trio binning approach. The genome sequence is 2,739 megabases in span. The majority of the assembly (95.16%) is scaffolded into 23 chromosomal pseudomolecules with the X and Y sex chromosomes assembled. The complete mitochondrial genome was also assembled and is 16.4 kilobases in length.

Apex carnivores are wide‐ranging, low‐density, hard to detect, and declining throughout most of their range, making population monitoring both critical and challenging. Rapid and inexpensive index calibration survey (ICS) methods have been developed to monitor large African carnivores. ICS methods assume constant detection probability and a predictable relationship between the index and the actual population of interest. The precision and utility of the resulting estimates from ICS methods have been questioned. We assessed the performance of one ICS method for large carnivores—track counts—with data from two long‐term studies of African lion populations. We conducted Monte Carlo simulation of intersections between transects (road segments) and lion movement paths (from GPS collar data) at varying survey intensities. Then, using the track count method we estimated population size and its confidence limits. We found that estimates either overstate precision or are too imprecise to be meaningful. Overstated precision stemmed from discarding the variance from population estimates when developing the method and from treating the conversion from tracks counts to population density as a back‐transformation, rather than applying the equation for the variance of a linear function. To effectively assess the status of species, the IUCN has set guidelines, and these should be integrated in survey designs. We propose reporting the half relative confidence interval width (HRCIW) as an easily calculable and interpretable measure of precision. We show that track counts do not adhere to IUCN criteria, and we argue that ICS methods for wide‐ranging low‐density species are unlikely to meet those criteria. Established, intensive methods lead to precise estimates, but some new approaches, like short, intensive, (spatial) capture–mark–recapture (CMR/SECR) studies, aided by camera trapping and/or genetic identification of individuals, hold promise. A handbook of best practices in monitoring populations of apex carnivores is strongly recommended.

Individual variation in survival probability due to differential responses to early‐life environmental conditions is important in the evolution of life‐histories and senescence. A biomarker allowing quantification of such individual variation, and which links early‐life environmental conditions with survival by providing a measure of conditions experienced, is telomere length. Here, we examined telomere dynamics among 24 cohorts of European badgers (Meles meles). We found a complex cross‐sectional relationship between telomere length and age, with no apparent loss over the first 29 months, but with both decreases and increases in telomere length at older ages. Overall, we found low within‐individual consistency in telomere length across individual lifetimes. Importantly, we also observed increases in telomere length within individuals, which could not be explained by measurement error alone. We found no significant sex differences in telomere length, and provide evidence that early‐life telomere length predicts lifespan. However, while early‐life telomere length predicted survival to adulthood (≥1 year old), early‐life telomere length did not predict adult survival probability. Furthermore, adult telomere length did not predict survival to the subsequent year. These results show that the relationship between early‐life telomere length and lifespan was driven by conditions in early‐life, where early‐life telomere length varied strongly among cohorts. Our data provide evidence for associations between early‐life telomere length and individual life‐history, and highlight the dynamics of telomere length across individual lifetimes due to individuals experiencing different early‐life environments.

Servals Leptailurus serval have a widespread distribution across sub-Saharan Africa with two large gaps: one in the tropical forest block of central Africa and one in the arid western block of southern Africa. We present new camera trap records of servals that fall within a large portion of the latter gap, including records from Khutse Game Reserve and Ghanzi that are more than 100 km outside the known range of the serval and may suggest a Kalahari-wide distribution.

The role of disease in population regulation is often overlooked in ecology and conservation. Due to their diversity, the musteloids host a wide range of pathogens. These include diseases of commercial importance, such Aleutian mink disease virus which impacts mink ranching, or bovine tuberculosis leading to interventions to manage European badgers. Skunks and raccoons are major rabies hosts in North America, and because these small carnivores insinuate themselves into close proximity with people, they can pose substantial zoonotic risks. Musteloids also share diseases between species, such as mustelid herpes virus, canine distemper and infectious hepatitis viruses, along with a range of nematodes and protozoans; presenting a contagion risk when vulnerable musteloids are being conserved or reintroduced. Managing host density, vaccination and host isolation are thus the best tools for managing disease, where we advocate the UN-led ‘One Health approach, aimed at reducing risks of infectious diseases at the Animal-Human-Ecosystem interface

Trophy hunting of wild lions (Panthera leo) is controversial - its impact on conservation has been much debated. It involves the hunting of selected individuals for sport and usually taking their body parts for display. The US recently listed the African lion on the Endangered Species Act, and the United States Fisheries and Wildlife Service (USFWS) now permits the import of lion trophies only from areas where hunting can be demonstrated to benefit conservation. The rationale is that many US lion hunters (currently the majority of lion hunters) are likely to stop hunting if they cannot take their trophies home. This threat provides a financial incentive for hunting operators to fulfil the prescribed requirements. The burden of proof is considerable – operators need to demonstrate population monitoring and ‘scientifically based management programs’; furthermore, they are required to show that local people benefit . Here, we contemplate the implications of applying consistent standards to any land use depending on wildlife.

Of thirteen extant species of true badger, eleven have a distribution in Asia, as do the more loosely affiliated stink- and honey-badgers. Even though these badgers show superficial similarities, they exhibit very different societies, even within same species under different circumstances, and provide an informative model to advance understanding of socio-ecology. They illustrate how group-living is promoted by natal philopatry, and food security; enabled by omnivory and hibernation in cold-winter regions. Conversely predatory, carnivorous species, and those competing for food security within a broader trophic guild, tend to be more solitary. This socio-ecological diversity poses conservation challenges, with Asian badgers vulnerable to habitat loss, urban and road development, direct conflict with people, culling to manage zoonotic disease transmission, and hunting pressure – often for traditional medicine. These threats are ever-more prevalent in expanding Asian economies, where cultural and attitudinal changes are urgently needed to safeguard biodiversity for the future.

Excluded from the pursuit predator niche by better-adapted early felids and canids, the musteloids exploited other hunting strategies as grasslands proliferated in the Oligocene. Unconstrained by specialised running limbs, lineages evolved to excavate prey (badgers) and enter burrows (polecats). Others took to tree-climbing (martens, procynoids) and even swimming (otters). While some species specialised in rodent hunting (weasels) others became more generalist omnivores. In-turn the dispersion of these food types dictated socio-spatial geometries, allowing insectivorous, piscivorous and frugivorous species to congregate with varying degrees of social cohesion, often unified within subterranean burrows – a basis to group-living distinct from the pack-hunting felids and canids. Induced ovulation and delayed implantation feature in the mating systems of several species, evolved to ensure breeding success amongst low-density, solitary ancestors. Group-living musteloids exhibit degrees of reproductive suppression, allo-parental care and other cooperative behaviours, thus this contrarian superfamily provides unique insights into the basis of carnivore societies.

European mink were once widespread across Eurasia, but now exist only in small isolated populations in parts of their former range. A number of factors likely contributed to the decline but direct inter-specific aggression from non-native American mink has been key. This chapter reviews restoration efforts for this species undertaken over the past 17 years on the (American mink-free) Estonian Island of Hiiumaa (where captive-bred European mink have been released in an attempt to create an island sanctuary) and in Spain (one of the few European countries were a viable population of European mink may persist). It discusses some of the challenges association with reintroduction efforts, including the potential role of personality types, and reviews the status of European mink in Spain, focusing on the threat associated with the current expansion of American mink there and the urgent need for conservation action.

The concluding chapter of this book poses the question: what is special about the musteloids in the context of promoting their conservation. Ranking species on their public appeal, most musteloids score relatively poorly as ambassadors, or flagships, for conservation compared with, for example, big cats. There are individual exceptions (many of the otters, for example, and the endangered ‘celebrity’ species, such as black-footed ferrets or red pandas), and some have potential as umbrella species due to their range overlap with other threatened mammals. The chapter explores if and how musteloids contribute to ecosystem services, and thus their utility value, and assesses the potential for identifying priorities for conservation action (in terms of both priority species and priority countries). The conservation needs of musteloids are varied, and these are not the only important considerations, but they illustrate some of the complexity that is involved.

Long-term research on Fosa Cryptoprocta ferox has been conducted in Ankarafantsika National Park since 1999, with rare sightings of the forest’s second elusive carnivore, the Western Falanouc Eupleres major. During annual carnivore live-trapping from 1999- Present, a single Falanouc was captured on the 30th of March 2002. Since this capture, two photographs have been taken of the Falanouc. In June 2011, a project volunteer photographed a Falanouc during the day whilst conducting trap-checks. Recently, a second photograph was recorded during the largest systematic camera-trapping study of Madagascar’s western carnivores. From April – July 2014 eighty paired camera-traps operated on average for 79 days, recording a sole image of the Western Falanouc at 01h18 on the 19 April 2014. Herein we discuss the morphometrics and photographic records of the Western Falanouc from Ankarafantsika National Park.

What does trophy hunting contribute to wild lion conservation? What constitutes best practice? D.W. Macdonald (2016) summarises what we know. However, in the much, but perhaps unfairly, mocked words of Donald Rumsfeld, there are knowns, known unknowns and unknown unknowns. We identified gaps in knowledge inhibiting conservation planning in several areas: these areas included the causes of lion mortality, the amount of land used for lion trophy hunting, the extent to which trophy hunting depends on lions for financial viability, and the vulnerability of areas currently used for trophy hunting to conversion to non wildlife-based land uses if it were to cease.

Overlap in resource use between cattle and wild herbivores have implications for conservation, particularly if food is limited. The aim of this paper is to investigate the diets of a wild and a domestic herbivore living in sympatry in plant communities degraded by woody encroachment. Dung samples from both species were subjected to microhistological analysis, making of this work the first study describing and comparing the diet of sympatric guanacos and cattle in the Chaco region. We found a relatively high richness of 53 plant species in the diets of both herbivores, and 4 species were exclusive to cattle. A clear separation between the diets of guanaco and cattle was observed at two levels using analysis of similarity (ANOSIM): a) species level (r=0.52, p=

Large carnivores are persecuted globally because they threaten human industries and livelihoods. How this conflict is managed has consequences for the conservation of large carnivores and biodiversity more broadly. Mitigating human–predator conflict should be evidence-based and accommodate people's values while protecting carnivores. Despite much research into human and large-carnivore coexistence strategies, there have been few attempts to document the success of conflict-mitigation strategies on a global scale. We conducted a meta-analysis of global research on conflict mitigation related to large carnivores and humans. We focused on conflicts that arise from the threat large carnivores pose to livestock. We first used structured and unstructured searching to identify replicated studies that used before–after or control–impact design to measure change in livestock loss as a result of implementing a management intervention. We then extracted relevant data from these studies to calculate an overall effect size for each intervention type. Research effort and focus varied among continents and aligned with the histories and cultures that shaped livestock production and attitudes toward carnivores. Livestock guardian animals most effectively reduced livestock losses. Lethal control was the second most effective control, although its success varied the most, and guardian animals and lethal control did not differ significantly. Financial incentives have promoted tolerance of large carnivores in some settings and reduced retaliatory killings. We suggest coexistence strategies be location-specific, incorporate cultural values and environmental conditions, and be designed such that return on financial investment can be evaluated. Improved monitoring of mitigation measures is urgently required to promote effective evidence-based policy.

In a world of shrinking habitats and increasing competition for natural resources, potentially dangerous predators bring the challenges of coexisting with wildlife sharply into focus. Through interdisciplinary collaboration between authors trained in the humanities, social sciences and natural sciences, this paper offers a review of current approaches and a vision for future approaches to understanding and mitigating adverse human-predator encounters. The paper first reviews some limitations to current approaches to mitigation. Second, it reviews an emerging interdisciplinary literature, identifying key perspectives on how to better frame and therefore successfully mitigate such conservation conflicts. Third, it discusses the implications for future research and management practice. It is concluded that a demand for rapid, ‘win-win’ solutions for conservation and development favours dispute resolution and technical fixes, obscuring important underlying drivers of conflicts. Without due cognisance of these underlying drivers, our well intentioned efforts, focussed on ‘human wildlife conflicts,’ will fail.

Using surveys of experts associated with 186 sites across 24 countries, we assessed the effectiveness of African protected areas (PAs) at conserving lions and their prey, identified factors that influence conservation effectiveness, and identified patterns in the severity of various threats. Less than one third of sampled PAs conserve lions at ≥ 50% of their estimated carrying capacity (K), and less than half conserve lion prey species at ≥ 50% of K. Given adequate management, PAs could theoretically support up to 4x × the total extant population of wild African lions (~ 83,000), providing a measurable benchmark for future conservation efforts. The performance of PAs shows marked geographic variation, and in several countries there is a need for a significant elevation in conservation effort. Bushmeat poaching was identified as the most serious threat to both lions and to wildlife in general. The severity of threats to wildlife in PAs and the performance of prey populations were best predicted by geographic-socioeconomic variables related to the size of PAs, whether people were settled within PAs, human/livestock densities in neighbouring areas and national economic indicators. However, conservation outcomes for lions were best explained by management variables. PAs tended to be more effective for conserving lions and/or their prey where management budgets were higher, where photographic tourism was the primary land use, and, for prey, where fencing was present. Lions and prey fared less well relative to their estimated potential carrying capacities in poorer countries, where people were settled within PAs and where PAs were used for neither photographic tourism nor trophy hunting.

Behavioural events that are important for understanding sociobiology and movement ecology are often rare, transient and localised, but can occur at spatially distant sites e.g. territorial incursions and co-locating individuals. Existing animal tracking technologies, capable of detecting such events, are limited by one or more of: battery life; data resolution; location accuracy; data security; ability to co-locate individuals both spatially and temporally. Technology that at least partly resolves these limitations would be advantageous. European badgers (Meles meles L.), present a challenging test-bed, with extra-group paternity (apparent from genotyping) contradicting established views on rigid group territoriality with little social-group mixing. In a proof of concept study we assess the utility of a fully automated Active-Radio-Frequency-Identification (aRFID) system combining badger-borne aRFID-tags with static, wirelessly-networked, aRFID-detector base-stations to record badger co-locations at setts (burrows) and near notional border latrines. We summarise the time badgers spent co-locating within and between social-groups, applying network analysis to provide evidence of co-location based community structure, at both these scales. The aRFID system co-located animals within 31.5 m (adjustable) of base-stations. Efficient radio transmission between aRFIDs and base-stations enables a 20 g tag to last for 2-5 years (depending on transmission interval). Data security was high (data stored off tag), with remote access capability. Badgers spent most co-location time with members of their own social-groups at setts; remaining co-location time was divided evenly between intra- and inter-social-group co-locations near latrines and inter-social-group co-locations at setts.Network analysis showed that 20-100% of tracked badgers engaged in inter-social-group mixing per week, with evidence of trans-border super-groups, i.e., badgers frequently transgressed notional territorial borders. aRFID occupies a distinct niche amongst established tracking technologies. We validated the utility of aRFID to identify co-locations, social-structure and inter- group mixing within a wild badger population, leading us to refute the conventional view that badgers (social-groups) are territorial and to question management strategies, for controlling bovine TB, based on this model. Ultimately aRFID proved a versatile system capable of identifying social-structure at the landscape scale, operating for years and suitable for use with a range of species.

The innate immune system provides the primary vertebrate defence system against pathogen invasion, but it is energetically costly and can have immune pathological effects. A previous study in sticklebacks found that intermediate major histocompatibility complex (MHC) diversity correlated with a lower leukocyte coping capacity (LCC), compared to individuals with fewer, or many, MHC alleles. The organization of the MHC genes in mammals, however, differs to the highly duplicated MHC genes in sticklebacks by having far fewer loci. Using European badgers (Meles meles), we therefore investigated whether innate immune activity, estimated functionally as the ability of an individual’s leukocytes to produce a respiratory burst, was influenced by MHC diversity. We also investigated whether LCC was influenced by factors such as age-class, sex, body condition, season, year, neutrophil and lymphocyte counts, and intensity of infection with five different pathogens. We found that LCC was not associated with specific MHC haplotypes, MHC alleles, or MHC diversity, indicating that the innate immune system did not compensate for the adaptive immune system even when there were susceptible MHC alleles/haplotypes, or when the MHC diversity was low. We also identified a seasonal and annual variation of LCC. This temporal variation of innate immunity was potentially due to physiological trade-offs or temporal variation in pathogen infections. The innate immunity, estimated as LCC, does not compensate for MHC diversity suggests that the immune system may function differently between vertebrates with different MHC organizations, with implications for the evolution of immune systems in different taxa.

Spatially extensive patterns of bushmeat extraction (and the processes underlying these patterns) have not been explored. We used data from a large sample (n= 87) of bushmeat trading points in urban and rural localities in Nigeria and Cameroon to explore extraction patterns at a regional level. In 7,594 sample days, we observed 61,267 transactions involving whole carcasses. Rural and urban trading points differed in species for sale and in meat condition (fresh or smoked). Carcass price was principally associated with body mass, with little evidence that taxonomic group (primate, rodent, ungulate, or mammalian carnivore) affected price. Moreover, meat condition was not consistently associated with price. However, some individual species were more expensive throughout the region than would be expected for their size. Prices were weakly positively correlated with human settlement size and were highest in urban areas. Supply did not increase proportionally as human settlement size increased, such that per capita supply was significantly lower in urban centers than in rural areas. Policy options, including banning hunting of more vulnerable species (those that have low reproductive rates), may help to conserve some species consumed as bushmeat because carcass prices indicate that faster breeding, and therefore the more sustainable species, may be substituted and readily accepted by consumers.

There may be conditions under which trophy hunting can contribute to the conservation of wildlife, but there are cases where its sustainability has been questioned. Several studies have shown that humans place value on rarity, and this may fuel the exploitation of rare species and increase their risk of extinction - a process termed the anthropogenic Allee effect. Previous studies have shown that some rarer species may command higher trophy prices than do broadly similar but less rare ones. Increase in rarity (as measured by International Union for the Conservation of Nature (IUCN) status)) has also been linked to increases in demand as reflected in the volume of recorded trophy hunts. We hypothesized that change in rarity, as measured by change in IUCN status between 2004 and 2010, would have an effect on price if increased demand resulted from the change. Change in rarity was an important predictor of changes in price between 2004 and 2010. Bovids that became more threatened had larger increases in trophy prices than those that did not. Whether this has positive or negative implications for conservation may depend on how well managed the trophy quota setting systems are. © 2011 The Authors. Animal Conservation © 2011 The Zoological Society of London.

Long-term studies on large felids are rare and yet they yield data essential to understanding the behaviour of species and the factors that facilitate their conservation. We used the most extensive data set so far compiled on leopards Panthera pardus to establish baseline reproductive parameters for females and to determine the demographic and environmental factors that affect their lifetime reproductive success. We used comprehensive sightings reports and photographs from ecotourism lodges in the Sabi Sand Game Reserve, South Africa, to reconstruct life histories for 44 female leopards that gave birth to 172 litters over a 32-year period. Leopards appeared to exhibit a birth pulse; most litters were born in the wet season, particularly in December. Mean age at first parturition (n=26, mean±standard error=46±2 months, range=33-62) was older than previously recorded, possibly due to elevated intraspecific competition. Average litter size was 1.9±0.1 (n=140, range=1-3) and declined with maternal age. Age of litters at independence (n=52, 19±1 months, range=9-31) was inversely related to prey abundance but did not affect the likelihood of recruitment of offspring. Interbirth intervals differed following successful litters (in which at least one cub survived to independence; n=55, 25±1 months, range=14-39) and unsuccessful litters (n=46, 11±1 months, range=4-36), as did the time taken to replace litters. Variation in lifetime reproductive success was influenced mainly by differences in cub survival, which was related to maternal age and vulnerability to infanticide. Cub survival (37%) declined as females got older, perhaps because mothers relinquished portions of their home ranges to philopatric daughters. Male leopards were responsible for many (40%) cub deaths and females appeared to adopt severalstrategies to counter the risk of infanticide, including paternity confusion and displaying a period of reduced fertility immediately after a resident male was replaced. Our results suggest that the reproductive success of female leopards is regulated primarily by top-down processes. This should be taken into account in management decisions, particularly when managers are considering the implementation of invasive activities such as legal trophy hunting. © 2012 The Authors. Mammal Review © 2012 John Wiley and Sons Ltd and The Mammal Society.

Bushmeat consumption is affected by household wealth. However, how household wealth impacts bushmeat eaten in different environmental and social settings (i.e. whether urban, rural, coastal or forest) is poorly understood. In this study, we sampled households in six contrasting localities in Rio Muni, Equatorial Guinea, in coastal (Bata, Cogo), central (Niefang, Evinayong) and eastern parts of the territory (Ebebiyin, Nsork). On average, 32.3 g of bushmeat per adult male equivalent per day were consumed, though this varied widely between sites and most households ate no bushmeat on the survey day. Fish was the most frequently recorded source of protein and in a coastal site, Cogo, significantly more fish was consumed than in the other localities. Overall, average protein consumption was correlated with household wealth, but the strength of this effect varied among sites. At the site where average wealth was highest (Bata, the most urban site), bushmeat was more expensive, and wealthier households ate more of it. Elsewhere bushmeat consumption was not associated with wealth, and the cost of bushmeat was a higher proportion of household wealth. In Bata, wealthier households reported consumption of more than one meat type (most frequently bushmeat and either domestic meat or fish), and diversity of dietary items also increased with wealth. In all sites, wealthier households ate less fish. We demonstrate distinct differences in relationships between urban versus rural areas, and between coastal versus inland sites. We therefore caution that general patterns of wealth-wild meat consumption must be evaluated taking account the circumstances of wild meat consumers. © 2009 The Authors. Journal compilation © 2009 The Zoological Society of London.

Livestock inside the Bale Mountains National Park poses a threat to the persistence of the Ethiopian wolf (Canis simensis) through grazing-induced habitat degradation and the transmission of diseases from the domestic dogs kept alongside the herds. We used a 21-year time series to explore long-term trends in the numbers of cattle, caprines and free-roaming domestic dogs in two core Ethiopian wolf areas (the Web valley and the Sanetti plateau) and to test whether seasonal variations in primary productivity underlies the current livestock production system. No trends in livestock numbers were detected in the Web valley, where livestock are most abundant and graze seasonally. Livestock numbers have increased significantly on most of the Sanetti plateau, grazing all year-round albeit at a lower intensity. Livestock use of the Web valley was positively correlated with vegetation productivity as derived from remotely sensed data, the Normalized Difference Vegetation Index (NDVI). On the Sanetti plateau, neither primary productivity nor livestock numbers showed signs of strong seasonality. The current livestock production system has the potential to degrade the vegetation that sustains the wolves' rodent prey while an increase in free-roaming domestic dogs in parts of their range may heighten the risk of disease transmission. +Résumé: Le bétail qui paît dans le Parc National des Bale Mountains est une menace pour la survie du loup éthiopien Canis simensis parce que le pâturage entraine une dégradation de l'habitat et favorise la transmission de maladies des chiens domestiques qui accompagnent les troupeaux. Nous avons utilisé une série de données couvrant 21 années pour étudier les tendances à long terme du nombre de bovins, de chèvres et de chiens domestiques parcourant librement deux zones clés pour les loups d'Ethiopie, la Vallée de Web et le Plateau de Sanetti, et pour tester si des variations saisonnières de productivité primaire sous-tendent le système actuel de production de bétail. Nous n'avons détecté aucune tendance dans le nombre de têtes de bétail dans la Vallée de Web, où le bétail est le plus abondant et où il paît de façon saisonnière. Le nombre de bêtes a augmenté significativement sur la plus grande partie du Plateau de Sanetti, où elles paissent toute l'année quoique à plus faible intensité. La fréquentation de la Vallée de Web était positivement liée à la productivité de la végétation selon des données recueillies à distance (NDVI). Sur le Plateau de Sanetti, ni la productivité primaire, ni le nombre de têtes de bétail ne présente de signe fort de saisonnalité. Le système actuel de production du bétail a le potentiel de dégrader la végétation permettant le maintien du rongeur qui est la proie du loup d'Ethiopie, cependant que la libre circulation des chiens domestiques dans plusieurs parties de leur aire de répartition pourrait accroître le risque de transmission de maladies. © 2010 Blackwell Publishing Ltd.

The potential of human activities, including research, to alter parasite transmission ecology in wildlife is unknown. We examined gastrointestinal parasitism in chimpanzees (Pan troglodytes schweinfurthii) in Budongo Forest, Uganda. Trail use and time spent on the ground was recorded during 10 months of observations in four sites with differing human disturbance. Disturbance was quantified using transect plots (n = 320). Fecal (n = 435) samples were examined for helminth eggs, larvae, and for protozoan cysts. Individuals that spent more time on the ground had more infections and higher intensity infections. Prevalence of 13 parasite species was similar across sites, but percentage of multiple infections and infection intensity differed, as did ground use. Chimpanzees at the long-term research site spent more time on the ground or on human trails. We hypothesize that researcher presence and trail creation may influence ground use, and thereby parasite burden, by altering trade-offs between foraging and predation risk. © 2013 Wiley Periodicals, Inc.

1. Crayfish are amongst the most frequently introduced non-native aquatic organisms, with well-documented negative effects on a large number of freshwater taxa. Many crayfish-control strategies make use of manual removal by trapping, a method known preferentially to remove the largest individuals, leaving the juvenile population almost entirely untrapped. 2. A predicted outcome of trapping bias in riparian habitats is that removed individuals could be replaced by large crayfish immigrating from surrounding, untrapped, areas. We tested the hypothesis that removal by trapping of American signal crayfish from a UK river would result in increased rates of immigration, and increased distances moved, of crayfish from untrapped areas. 3. We studied four stretches of the River Windrush each 1 km in length and divided into three sections; a 250 m long upstream section, a 500 m middle section and a 250 m downstream section. At two sites (removal sites) signal crayfish were trapped and removed from the 500 m middle sections, at the other two (non-removal) they were marked and returned. All crayfish captured in the upstream and downstream sections were marked and returned. 4. Probability of capture was higher for larger individuals with both chelae intact, and larger crayfish were more likely to immigrate from the upstream and downstream sections into the middle. The percentage of captured crayfish immigrating into the middle sections was the same (3.7%) in both removal and non-removal sites. However, the mean distance that crayfish moved when immigrating was significantly greater at removal sites (239 m) than at non-removal sites (187 m). 5. These results imply that removal of large individuals may have reduced the potential for interference competition by increasing the relative competitiveness of the immigrating individuals and permitting them to make larger movements. Consequently, the impact of manual removal strategies, both on the signal crayfish population and other biota affected by them, is likely to be reduced at the point of removal, but to extend at least 200 m beyond the trapped length of river.

As sensor network technologies become more mature, they are increasingly being applied to a wide variety of applications, ranging from agricultural sensing to cattle, oceanic and volcanic monitoring. Significant efforts have been made in deploying and testing sensor networks resulting in unprecedented sensing capabilities. A key challenge has become how to make these emerging wireless sensor networks more sustainable and easier to maintain over increasingly prolonged deployments. In this paper, we report the findings from a one year deployment of an automated wildlife monitoring system for analyzing the social co-location patterns of European badgers (Meles meles) residing in a dense woodland environment. We describe the stages of its evolution cycle, from implementation, deployment and testing, to various iterations of software optimization, followed by hardware enhancements, which in turn triggered the need for further software optimization. We report preliminary descriptive analyses of a subset of the data collected, demonstrating the significant potential our system has to generate new insights into badger behavior. The main lessons learned were: the need to factor in the maintenance costs while designing the system; to look carefully at software and hardware interactions; the importance of a rapid initial prototype deployment (this was key to our success); and the need for continuous interaction with domain scientists which allows for unexpected optimizations. Copyright 2010 ACM.

The vicuna is mainly used in two ways: wild captured, shorn and returned to the wild; or wild captured and maintained in captivity as part of a programme of sustainable use in the Andes of South America. Farming of wild vicunas has hitherto involved no assessment of their welfare. In this study we measured a set of basic blood parameters in order to characterise baseline values in captivity, and we then characterised adrenal cortical responsiveness using an ACTH challenge. The ACTH challenge is widely used for assessing neuroendocrine responses to stress and is now increasingly being applied to studies of wild animals’ welfare. Five male vicunas were injected with exogenous ACTH and their responses compared with those of a control group injected with placebo. Behavioural and haematological changes were monitored. Injection of ACTH produced a 4.5-fold increase in cortisol concentration within 1 h. Total white blood cell count almost doubled in less than 5 h. The neutrophil:lymphocyte ratio also changed, with a decrease in lymphocytes and an increase in neutrophils, suggesting that the neutrophil:lymphocyte ratio was affected by the ACTH challenge. Packed cell volume increased from 40% to 44%. Observations of individual vicunas during sampling revealed no discernible behavioural differences between treated and control animals; however, animals that had higher initial baseline cortisol concentration made more attempts to escape, and vocalised more during handling, regardless of whether they were treated with ACTH or placebo. The results reveal the different blood parameter levels associated with stress in different species and highlight the hazard of interpreting stress levels in one species on the basis of measures calibrated in another. We provide calibrated reference values for future studies of stress in vicunas.