1. A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocytes
- Author
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Magali Seguret, Patricia Davidson, Stijn Robben, Charlène Jouve, Celine Pereira, Quitterie Lelong, Lucille Deshayes, Cyril Cerveau, Maël Le Berre, Rita S Rodrigues Ribeiro, and Jean-Sébastien Hulot
- Subjects
tissue engineering ,organoids ,heart ,cardiac myocytes ,induced pluripotent stem cells ,drug screening ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
We developed a 96-well plate assay which allows fast, reproducible, and high-throughput generation of 3D cardiac rings around a deformable optically transparent hydrogel (polyethylene glycol [PEG]) pillar of known stiffness. Human induced pluripotent stem cell-derived cardiomyocytes, mixed with normal human adult dermal fibroblasts in an optimized 3:1 ratio, self-organized to form ring-shaped cardiac constructs. Immunostaining showed that the fibroblasts form a basal layer in contact with the glass, stabilizing the muscular fiber above. Tissues started contracting around the pillar at D1 and their fractional shortening increased until D7, reaching a plateau at 25±1%, that was maintained up to 14 days. The average stress, calculated from the compaction of the central pillar during contractions, was 1.4±0.4 mN/mm2. The cardiac constructs recapitulated expected inotropic responses to calcium and various drugs (isoproterenol, verapamil) as well as the arrhythmogenic effects of dofetilide. This versatile high-throughput assay allows multiple in situ mechanical and structural readouts.
- Published
- 2024
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