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Background and aimShift work, especially including night shifts, has been found associated with several diseases, including obesity, diabetes, cancers, and cardiovascular, mental, gastrointestinal and sleep disorders. Metabolomics (an omics-based methodology) may shed light on early biological alterations underlying these associations. We thus aimed to evaluate the effect of night shift work (NSW) on serum metabolites in a sample of hospital female nurses.MethodsWe recruited 46 nurses currently working in NSW in Milan (Italy), matched to 51 colleagues not employed in night shifts. Participants filled in a questionnaire on demographics, lifestyle habits, personal and family health history and work, and donated a blood sample. The metabolome was evaluated through a validated targeted approach measuring 188 metabolites. Only metabolites with at least 50% observations above the detection limit were considered, after standardization and log-transformation. Associations between each metabolite and NSW were assessed applying Tobit regression models and Random Forest, a machine-learning algorithm.ResultsWhen comparing current vs. never night shifters, we observed lower levels of 21 glycerophospholipids and 6 sphingolipids, and higher levels of serotonin (+171.0%, 95%CI: 49.1–392.7), aspartic acid (+155.8%, 95%CI: 40.8–364.7), and taurine (+182.1%, 95%CI: 67.6–374.9). The latter was higher in former vs. never night shifters too (+208.8%, 95%CI: 69.2–463.3). Tobit regression comparing ever (i.e., current + former) and never night shifters returned similar results. Years worked in night shifts did not seem to affect metabolite levels. The Random-Forest algorithm confirmed taurine and aspartic acid among the most important variables in discriminating current vs. never night shifters.ConclusionsThis study, although based on a small sample size, shows altered levels of some metabolites in night shift workers. If confirmed, our results may shed light on early biological alterations that might be related to adverse health effects of NSW.

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La Pace di Chiavenna è un evangelario attribuito stilisticamente e iconograficamente all'età ottoniana (950-1050). È composto da un telaio ligneo rivestito da lamine d'oro lavorate, gemme e smalti cloisonné, questi ultimi fissati alla struttura tramite un composto di cera d'api. Non sono state rinvenute notizie certe sull'esatta data di produzione dell'opera, anche se il lavoro più simile stilisticamente è l'evangelario dell'arcivescovo milanese Ariberto da Intimiano (1018-1045). Durante una campagna di restauro promossa dall'Università Cattolica è stato possibile estrarre due carote di legno dal telaio, un campione di cera da uno smalto, e una scheggia di legno da una croce posta sotto il rosone centrale della copertina. I campioni sono stati pretrattati ed analizzati con uno spettrometro di massa con acceleratore per essere datati con radiocarbonio AMS. Sui due campioni di legno provenienti dal telaio è stato possibile effettuare anche una calibrazione dendrocronologica wiggle-matching, essendo ben visibili gli anelli di separazione tra una zona di prelievo e l'altra. I risultati sono stati confrontati con analisi di tipo archeobotanico e palinologico. Nel lavoro vengono presentati risultati, commenti e conclusioni. In particolare verrà mostrato come tutte le analisi concorrono ad inserire l'opera in un contesto ottoniano, e a validare quindi la prima interpretazione stilistica effettuata sull'opera.

"Chronoastrobiology: are we at the threshold of a new science? Is there a critical mass for scientific research?" A simple photograph of the planet earth from outer space was one of the greatest contributions of space exploration. It drove home in a glance that human survival depends upon the wobbly dynamics in a thin and fragile skin of water and gas that covers a small globe in a mostly cold and vast universe. This image raised the stakes in understanding our place in that universe, in finding out where we came from and in choosing a path for survival. Since that landmark photograph was taken, new astronomical and biomedical information and growing computer power have been revealing that organic life, including human life, is and has been connected to invisible (non-photic) forces, in that vast universe in some surprising ways. Every cell in our body is bathed in an external and internal environment of fluctuating magnetism. It is becoming clear that the fluctuations are primarily caused by an intimate and systematic interplay between forces within the bowels of the earth--which the great physician and father of magnetism William Gilbert called a 'small magnet'--and the thermonuclear turbulence within the sun, an enormously larger magnet than the earth, acting upon organisms, which are minuscule magnets. It follows and is also increasingly apparent that these external fluctuations in magnetic fields can affect virtually every circuit in the biological machinery to a lesser or greater degree, depending both on the particular biological system and on the particular properties of the magnetic fluctuations. The development of high technology instruments and computer power, already used to visualize the human heart and brain, is furthermore making it obvious that there is a statistically predictable time structure to the fluctuations in the sun's thermonuclear turbulence and thus to its magnetic interactions with the earth's own magnetic field and hence a time structure to the magnetic fields in organisms. Likewise in humans, and in at least those other species that have been studied, computer power has enabled us to discover statistically defined endogenous physiological rhythms and further direct effects that are associated with these invisible geo- and heliomagnetic cycles. Thus, what once might have been dismissed as noise in both magnetic and physiological data does in fact have structure. And we may be at the threshold of understanding the biological and medical meaning and consequences of these patterns and biological-astronomical linkages as well. Structures in time are called chronomes; their mapping in us and around us is called chronomics. The scientific study of chronomes is chronobiology. And the scientific study of all aspects of biology related to the cosmos has been called astrobiology. Hence we may dub the new study of time structures in biology with regard to influences from cosmo- helio- and geomagnetic rhythms chronoastrobiology. It has, of course, been understood for centuries that the movements of the earth in relation to the sun produce seasonal and daily cycles in light energy and that these have had profound effects on the evolution of life. It is now emerging that rhythmic events generated from within the sun itself, as a large turbulent magnet in its own right, can have direct effects upon life on earth. Moreover, comparative studies of diverse species indicate that there have also been ancient evolutionary effects shaping the endogenous chronomic physiological characteristics of life. Thus the rhythms of the sun can affect us not only directly, but also indirectly through the chronomic patterns that solar magnetic rhythms have created within our physiology in the remote past. For example, we can document the direct exogenous effects of given specific solar wind events upon human blood pressure and heart rate. We also have evidence of endogenous internal rhythms in blood pressure and heart rate that are close to but not identical to the period length of rhythms in the solar wind. These were installed genetically by natural selection at some time in the distant geological past. This interpretive model of the data makes the prediction that the internal and external influences on heart rate and blood pressure can reinforce or cancel each other out at different times. A study of extensive clinical and physiological data shows that the interpretive model is robust and that internal and external effects are indeed augmentative at a statistically significant level. Chronoastrobiological studies are contributing to basic science--that is, our understanding is being expanded as we recognize heretofore unelaborated linkages of life to the complex dynamics of the sun, and even to heretofore unelaborated evolutionary phenomena. Once, one might have thought of solar storms as mere transient 'perturbations' to biology, with no lasting importance. Now we are on the brink of understanding that solar turbulences have played a role in shaping endogenous physiological chronomes. There is even documentation for correlations between solar magnetic cycles and psychological swings, eras of belligerence and of certain expressions of sacred or religious feelings. Chronoastrobiology can surely contribute to practical applications as well as to basic science. It can help develop refinements in our ability to live safely in outer space, where for example at the distance of the moon the magnetic influences of the sun will have an effect upon humans unshielded by the earth's native magnetic field. We should be better able to understand these influences as physiological and mechanical challenges, and to improve our estimations of the effects of exposure. Chronoastrobiology moreover holds great promise in broadening our perspectives and powers in medicine and public health right here upon the surface of the earth. Even the potential relevance of chronoastrobiology for practical environmental and agricultural challenges cannot be ruled out at this early stage in our understanding of the apparently ubiquitous effects of magnetism and hence perhaps of solar magnetism on life. The evidence already mentioned that fluctuations in solar magnetism can influence gross clinical phenomena such as rates of strokes and heart attacks, and related cardiovascular variables such as blood pressure and heart rate, should illustrate the point that the door is open to broad studies of clinical implications. The medical value of better understanding magnetic fluctuations as sources of variability in human physiology falls into several categories: 1) The design of improved analytical and experimental controls in medical research. Epidemiological analyses require that the multiple sources causing variability in physiological functions and clinical phenomena be identified and understood as thoroughly as possible, in order to estimate systematic alterations of any one variable. 2) Preventive medicine and the individual patients'care. There are no flat 'baselines', only reference chronomes. Magnetic fluctuations can be shown statistically to exacerbate health problems in some cases. The next step should be to determine whether vulnerable individuals can be identified by individual monitoring. Such vulnerable patients may then discover that they have the option to avoid circumstances associated with anxiety during solar storms, and/or pay special attention to their medication or other treatments. Prehabilitation by self-help can hopefully complement and eventually replace much costly rehabilitation. 3) Basic understanding of human physiological mechanisms. The chronomic organization of physiology implies a much more subtle dynamic integration of functions than is generally appreciated. All three categories of medical value in turn pertain to the challenges for space science of exploring and colonizing the solar system. The earth's native magnetic field acts like an enormous umbrella that offers considerable protection on the surface from harsh solar winds of charged particles and magnetic fluxes. The umbrella becomes weaker with distance from the earth and will offer little protection for humans, other animals, and plants in colonies on the surface of the moon or beyond. Thus it is important before more distant colonization is planned or implemented to better understand those magnetism-related biological- solar interactions that now can be studied conveniently on earth. (ABSTRACT TRUNCATED)

Obiettivi: A seguito delle raccomandazioni degli ultimi PSN le Regioni hanno attivato programmi di screening sul cancro della mammella. In Lombardia la ASL-1 Provincia di Milano ha implementato un programma di screening monitorato attraverso gli indicatori di processo GISMa. L'obiettivo è verificare il grado di soddisfazione delle donne coinvolte per migliorare il programma. Metodi: È stata condotta un'indagine telefonica nell'Ottobre-Dicembre 2004 tra un campione casuale di 350 donne che avevano effettuato la mammografia nel Gennaio-Giugno 2004. Le telefonate sono avvenute in vari giorni feriali ad orari diversificati. Risultati: Di 301 partecipanti, 289 (96%) erano disponibili all'intervista. L'età mediana è 59 anni (range 52-74). Il giudizio complessivo sullo screening è buono/ottimo per il 90%. Circa il 50% delle intervistate riferisce di aver provato fastidio/sensazioni sgradevoli nell'effettuare la mammografia. Il 75-80% è correttamente informata su finalità e scopi dello screening. Le donne più giovani sono significativamente più disponibili a controllarsi anche in assenza di screening gratuito rispetto alle più anziane (= 60 anni). Non è risultata alcuna caratteristica individuale capace di discriminare efficacemente chi effettua l'autopalpazione (61%) e chi no. Fastidio e dolore riferiti nell'effettuare la mammografia non si distribuiscono casualmente tra i vari distretti ma evidenziano una maggior proporzione rispetto all'atteso in alcune realtà e condizionano significativamente il giudizio finale della donna. Conclusioni: Maggiore attenzione va posta nel proporre iniziative educative alla salute delle donne più anziane che sembrano recepire meno di altre il messaggio preventivo. L'esperienza personale della donna nella fase di esecuzione della diagnosi risulta tra i fattori più influenti sul suo giudizio complessivo e, di conseguenza, potrebbe rappresentare di per sé un elemento di distorsione capace di invalidare la corretta interpretazione di un'indagine di customer satisfaction per il miglioramento di una campagna di screening.

This study examines the early data of a breast cancer screening campaign (Progetto Donna) set up by the Milan Health Unit 1 in 1999. The authors have focused on some basic organizational aspects of such campaign and the patients' high response to the project showed a good efficiency of this initiative, aimed at informing and awakening people. The high rate of suspiciously positive results checked in several Hospitals, led to a careful assessment of the quality and the standards of radiology services.