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Unlike the chemical composition and diagenetic modification of buried bones, subaqueous archaeological bone diagenesis has not been studied in detail. This observational work presents a macroscopic and microscopic characterization of 11 variably preserved archaeological terrestrial mammalian bones submerged in seawater and/or surrounded by marine sediment for 169–347 years. In situ trace element analysis was undertaken to identify geochemical fingerprints of diagenesis. The analyzed bones belong to a collection of underwater archaeological faunal materials excavated from four shipwreck sites. With one exception, all archaeological bones were fragmented, some were also heavily stained, and in two samples, the damage to the cortical layer was extensive. Bioerosion was assessed by scanning electron microscopy (SEM), and bone trace element chemistry (by laser ablation inductively coupled mass spectrometry—LA-ICP-MS) was compared with that of an unsubmerged modern sheep bone control. In the control, several trace elements were low in concentration (weighted mean concentration <1 ppm; Cr, Co, Ni, Cu, Y, rare earth elements, Th, U). In the submerged archaeological bones, the weighted mean concentration of Li, Cr, Cu, and U was enriched relative to the modern sheep bone, whereas Rb and Ba were depleted. The best-preserved bone, recovered from Batavia, showed less variation in trace element patterns compared with the more poorly preserved bones. The only archaeological bone with preserved macroscopic structure and cortex showed a gradual decrease in trace element concentration from the outer surface towards the medullary cavity, whereas in samples where more cortical damage was noted, the distribution of these elements is more irregular. With the exception of Cu and Cr, the elements focused on in this work (Li, U, Rb, and Ba) are nonessential to life, supported by their low concentration in the modern sheep bone (with the exception of Ba). The results suggest that early macr

Learning Overview: After attending this presentation, attendees will have a better understanding of the impact and the artifacts produced by the activity of necrophagous entomofauna on stab-cuts and tear damage on different types of fabric during the postmortem period. Impact Statement: This presentation will impact the forensic science community by increasing awareness of the effect of carrion insects on clothing and by providing a new body of information that will enhance the investigative role of clothing associated with decomposed and skeletonized remains. Fatal stabbing incidents are the leading cause of homicides in countries with restricted access to firearms, such as Australia.1 During a stabbing assault, the distinctive characteristics of a sharp implement will deposit specific features.2 When the decomposition process impedes the physical examination of a stab wound, damage analysis of the clothing may provide information about the weapon or the actions that caused the injury.3 Studies have suggested that insect activity associated with decomposition can produce artifacts on textiles, modify perimortem textile damage or produce changes to clothing that imitate indicators of sexual homicides.4-7 However, the extent of such studies is currently limited and have not been conducted in Australia before. The aim of this research was to identify and characterize the effect of carrion insects on textile damage after a decomposition period during summer in Australia. The effect of insect activity was analyzed on standardized cuts and tears to three different fabrics (100% cotton, 65% polyester-35% cotton, 80% nylon- 20% spandex). Ninety stillborn piglets (Sus scrofa domesticus L.) were wrapped in one type of fabric each. Each clothed piglet was either stabbed by a stabbing apparatus or had its fabric torn. All piglets were placed simultaneously in a decomposition facility alongside controls of each combination of fabric and damage type, including 9 piglets clothed

The incidental sound produced by the oscillation of insect wings during flight provides an opportunity for species identification. Calyptrate flies include some of the fastest and most agile flying insects, capable of rapid changes in direction and the fast pursuit of conspecifics. This flight pattern makes the continuous and close recording of their wingbeat frequency difficult and limited to confined specimens. Advances in sound editor and analysis software, however, have made it possible to isolate low amplitude sounds using noise reduction and pitch detection algorithms. To explore differences in wingbeat frequency between genera and sex, 40 specimens of three-day old Sarcophaga crassipalpis, Lucilia sericata, Calliphora dubia, and Musca vetustissima were individually recorded in free flight in a temperature-controlled room. Results showed significant differences in wingbeat frequency between the four species and intersexual differences for each species. Discriminant analysis classifying the three carrion flies resulted in 77.5% classified correctly overall, with the correct classification of 82.5% of S. crassipalpis, 60% of C. dubia, and 90% of L. sericata, when both mean wingbeat frequency and sex were included. Intersexual differences were further demonstrated by male flies showing significantly higher variability than females in three of the species. These observed intergeneric and intersexual differences in wingbeat frequency start the discussion on the use of the metric as a communication signal by this taxon. The success of the methodology demonstrated differences at the genus level and encourages the recording of additional species and the use of wingbeat frequency as an identification tool for these flies.

The number of foreign citizens resident in Italy is approximately 5 million, making them the 8.7% of the resident population of the country. However, over the past 20 years, foreign citizens accounted for 24% of the total number of defendants involved in homicides, especially associated with robbery, disputes and assaults, generally connected to prostitution. Investigations of cases in the context of culturally diverse or minority communities can be particularly complex. This is due to the education, the cultural background and the cohesiveness of the community, alongside a negative view of law enforcement. These factors determine a relatively low resolution rate of these violent crimes in these communities. The investigation of the suspicious death of an Indian citizen living in Italy is presented in this paper. The body was discovered in a cattle shed, and despite the initial assumption of an accidental death, the cranial lesions were not consistent with the view that the death was caused by a kick by a cow. The results of a multidisciplinary analysis, including imaging, pathology, toxicology and veterinary forensics, were directed towards a homicide, with the manner of death ultimately filed as undetermined. During the course of the investigation, the collaboration between law enforcement and the community was thwart with difficulty, and as a consequence this case is currently unsolved. In order for law enforcement to continue to play a constructive and supportive role, it is imperative that every country invest in civil education of local population and of any ethnic groups, minorities and culturally diverse people.

In densely populated countries like Italy, cremation is promoted for the final disposition of the dead. However, many families still choose inhumation or entombment. In ordinary (traditional) tombs, bodies skeletonize slowly and partially, and often need a second disposal after the exhumation. The aim of this study was to experimentally test the functionality of a new type of tomb, defined as “aerated”. Aerated tombs feature an aerating system, absorbing materials and a purifying filter, which collectively maintain ventilation, process putrefactive fluids and gases and neutralize odors. In an experimental cemetery area with pristine soil, limbs of piglets were wrapped in cotton sheets and were either inhumed, placed in ordinary tombs or placed in aerated tombs. Following exhumation after planned time intervals (1, 3, 6, 9, 12, 18, 24 months), all samples were macro- and microscopically examined. The inhumed samples were completely skeletonized by 9 months after burial, and after 12 months showed initial bioerosion in bone Haversian canals. The traditionally entombed samples developed progressive adipocere formation, whereas the samples disposed in aerated tombs became mummified. Despite this outcome, aerated tombs represent a more energy-effective, environmentally-friendly and economical choice when compared to ordinary tombs. A mummified body is lighter and drier than a body entombed traditionally and, as such, it is easier to exhume and quicker to cremate. Overall, in the absence of alternative burials, aerated tombs are more suitable than ordinary tombs for the final disposition of the dead in cemeteries with limited space. The results of this experiment add to the knowledge of taphonomical processes in temperate climates and urban environments, potentially benefitting the forensic and medico-legal community.

Chrysomya rufifacies (Macquart), the hairy maggot blow fly, is of great importance for the field of forensic entomology due to its habit as an early colonizer of decomposing vertebrate remains and myiasis producer. Development studies on this species have been conducted in scattered regions of the world, using types of tissue from several species of animals as a rearing medium. Despite the commonality of C. rufifacies in Sri Lanka, developmental studies have never been performed in this region. As well, the effects of diet on development have not been tested. In the current study, C. rufifacies immatures were reared on skeletal muscle, liver, and heart from domestic swine, with flies from colonies maintained at 25 and 28°C. The minimum time needed to complete each stage at 25°C on liver (224.14 h) was fastest followed by skeletal muscle (249.33 h) and heart (251.64 h) respectively, whereas at 28°C, fly development was quickest on heart muscle (178.27 h) followed by liver (178.50 h) and skeletal muscle (186.17 h) respectively. A significant difference in total development time was determined for temperature, while the rearing medium was not significant. Temperature also showed a significant effect on the length and the width of the larvae, while the type of tissue statistically impacted only the width.

The Coronavirus disease 2019 (COVID-19) is spreading around the world, representing a global pandemic. In this context, governments from all around the world suspended all education, industry and business activities, alongside restricting the movement of people. Nevertheless, during this period, the activity of the law enforcement and the forensic investigators never stopped. At the present time, clinical and forensic autopsies of SARS-CoV-2 virus-positive cases, as well as handling of potentially infected biological samples in forensic practice have given guidance. However, less attention has been given to the development of specific adjustments to the existing crime scene investigation protocols and procedures for this exceptional time. This manuscript aims to share the methods and strategies adopted for the investigation of high priority criminal cases during the pandemic. Furthermore, other pandemic-related processes are critically explored, in order to propose adjustments for any forensic services to be prepared to face similar challenges in the future. The overall goal of this manuscript is to provide a summary of the main measures and the procedures developed to make the operations possible, while safeguarding the technicians in the field and the activity in the forensic laboratory. In order to minimize the risk of infection for personnel, adjustments to the standard practice have been proposed for each of the different phases of crime scene management, i.e. CSI call policy, equipment preparation, working groups, procedure at the scene, chain of custody and analyses of the evidence at the forensic lab. As this is a current study, based on limited cases and limited sources in the literature, changes and updates to the indications provided in this paper may be needed in the near future, according to new virologic data epidemiological trends.

Foraminifera are marine single-celled organisms, ubiquitous in marine environments, present in brackish waters and absent in terrestrial locations. Their presence has been associated with archaeological and forensic studies only rarely, and just once and superficially with bones of terrestrial mammals. In this study, a new association is presented between foraminifera enclosed in the dissolving trabecular spaces of terrestrial mammalian bones, recovered in underwater archaeological excavations between 1968 and 1980. Research on the new association aims to detail the micro-characterization of bone in underwater environments, leading to a better understanding of bone taphonomic trajectories, the chronological sequences of changes occurring between death and the incorporation of the remains of an organism within the depositional environment. The analysis of taphonomic trajectories is known to hold relevance in distinct disciplines, such as archaeology, palaeontology and forensic sciences. Different foraminiferal taxa are linked to different marine environments, characterized by specific ranges of water depth, amount of light and oxygen, temperature and composition of sediment. The association between foraminifera and terrestrial mammalian bones indicates deposition in a marine or brackish environment, thus the analysis of the specific ecology of the identified foraminiferal taxa can point to a specific environment, adding information to paleontological, archaeological or forensics casework.

Coronavirus infections have been a part of the animal kingdom for millennia. The difference emerging in the twenty-first century is that a greater number of novel coronaviruses are being discovered primarily due to more advanced technology and that a greater number can be transmitted to humans, either directly or via an intermediate host. This has a range of effects from annual infections that are mild to full-blown pandemics. This review compares the zoonotic potential and relationship between MERS, SARS-CoV, and SARS-CoV-2. The role of bats as possible host species and possible intermediate hosts including pangolins, civets, mink, birds, and other mammals are discussed with reference to mutations of the viral genome affecting zoonosis. Ecological, social, cultural, and environmental factors that may play a role in zoonotic transmission are considered with reference to SARS-CoV, MERS, and SARS-CoV-2 and possible future zoonotic events.

Blow-fly (Diptera: Calliphoridae) immatures are the main colonizers of decomposing remains, and any information on what influences their growth and development are important to forensic entomologists when they are required to estimate post-mortem intervals during a death investigation. Much of this work has been qualified and quantified in terrestrial environments, but is deplete in aquatic environments. When considering a blow-fly's life history, the longest immature life stage goes from the formation of the puparium to adult emergence, and involves metamorphosis. In an aquatic scenario a corpse may be completely submerged, floating on the surface and or it could be associated with water but neither submerged or floating (e.g. beached on a seashore or washed up after a flood event). The present study concerns two blow-fly species, Lucilia sericata (Meigen) and Calliphora vomitoria (L.), and the effects of the age of the intra-puparial forms (“pupal age”) and resultant survival, when submerged in tap, river or salt water for varying times – up to 3 days. The experiment was conducted in two localities, L. sericata in Boston USA and C. vomitoria in Turin, Italy, and full puparia of both species were divided into 4 age cohorts (“white”, “young”, “medium”, and “old’) before submergence. L. sericata intra-puparial forms showed a three time greater survival rate compared to C. vomitoria intra-puparial forms when submerged in each of the three water types. Both species had the highest survival rate in tap water. Overall, younger and older intra-puparial forms showed a greater and significant survival rate than medium intra-puparial forms when submerged. The eclosion time following submersion of C. vomitoria and L. sericata was mainly influenced by both the age at which the intra-puparial forms were submerged, and by the type of water, but the duration of the submersion also influenced the eclosion time of L. sericata. These results are discussed considering blow-fly physio

The estimation of the time since death (minimum Post Mortem Interval, minPMI) is an essential aspect of forensic investigations. This is particularly complex when a human body is found submerged, floating or beached in a marine environment. When a cadaver is found in a terrestrial environment the minPMI estimation is generally based on the presence of carrion insects. However, when a cadaver is found in an aquatic environment, a correct crime scene reconstruction is more complex and requires the consideration of the time the remains spent submerged underwater (minimum Post Mortem Submersion Interval, minPMSI) and/or floating (Floating Interval, FI). In marine crime scene scenarios, the use of barnacles (Crustacea: Cirripedia) has recently received some attention, due to their permanent settlement on human remains and their accompanying clothing. Previous research considered barnacle growth on human shoes, but the present research is the first to focus on the colonisation of barnacles on clothing materials (fabrics). Polystyrene floats were covered by either cotton, velvet, satin or neoprene and submerged underwater over a period of six months off the coast of Perth, Western Australia. The aims of this research were 1) the identification of marine species colonising the fabrics, with special attention to barnacles; 2) the identification of which fabric type provides the most desirable environment for colonisation; and 3) the identification of factors that affect the growth rate of the different species. Three species of barnacles, Balanus trigonus Darwin, Amphibalanus reticulatus (Utinomi) and A. variegatus (Darwin), were present in varying numbers and sizes. The colonisation process of the barnacles occurred rapidly, with the first sighting of barnacles observed within the first month on neoprene and control floats. The surface that attracted the largest number of barnacles was neoprene, followed by satin and cotton, while velvet showed an inconsistent colonisation

Next-generation sequencing (NGS) studies show that mosquito and tick microbiomes influence the transmission of pathogens, opening new avenues for vector-borne pathogen control. Recent microbiological studies of Australian ticks highlight fundamental knowledge gaps of tick-borne agents. This investigation explored the composition, diversity and prevalence of bacteria in Australian ticks (n = 655) from companion animals (dogs, cats and horses). Bacterial 16S NGS was used to identify most bacterial taxa and a Rickettsia-specific NGS assay was developed to identify Rickettsia species that were indistinguishable at the V1-2 regions of 16S. Sanger sequencing of near full-length 16S was used to confirm whether species detected by 16S NGS were novel. The haemotropic bacterial pathogens Anaplasma platys, Bartonella clarridgeiae, “Candidatus Mycoplasma haematoparvum” and Coxiella burnetii were identified in Rhipicephalus sanguineus (s.l.) from Queensland (QLD), Western Australia, the Northern Territory (NT), and South Australia, Ixodes holocyclus from QLD, Rh. sanguineus (s.l.) from the NT, and I. holocyclus from QLD, respectively. Analysis of the control data showed that cross-talk compromises the detection of rare species as filtering thresholds for less abundant sequences had to be applied to mitigate false positives. A comparison of the taxonomic assignments made with 16S sequence databases revealed inconsistencies. The Rickettsia-specific citrate synthase gene NGS assay enabled the identification of Rickettsia co-infections with potentially novel species and genotypes most similar (97.9–99.1%) to Rickettsia raoultii and Rickettsia gravesii. “Candidatus Rickettsia jingxinensis” was identified for the first time in Australia. Phylogenetic analysis of near full-length 16S sequences confirmed a novel Coxiellaceae genus and species, two novel Francisella species, and two novel Francisella genotypes. Cross-talk raises concerns for the MiSeq platform as a diagnostic tool for cl

In this case, a 43 years old mentally disabled man died of septic shock, caused by severe and untreated burns. He lived in an apartment in a flat complex, owned and also inhabited by his mother and various relatives who, beyond denying any knowledge of his living conditions, did not seek any medical assistance after a fire broke out into his apartment a few days before death. The carcasses of a dog, a cat and a turtle in different stages of decomposition were also found in the apartment. The following investigation, beyond cause, mode and manner of death, was directed to assess the timeline of the deceased’s mental disability, thus the duration of potential neglect from the family part. The investigation was multidisciplinary, and it revealed about 20 years of neglect of a person whose mental disability was completely unknown by health, social and welfare services. The veterinary forensics assessment determined that also the pets were neglected, and most likely starved to death.

A case of suicide by insulin overdose is apparently simple to handle and file, but it triggers more than a few questions and reflections, both because it is rare and because the postmortem assessment can be quite complex. In this example the deceased person was not immediately identified, and the body was found in the open and close to a water source (creek). Critical information, such as clinical and psychiatric history, and/or a farewell letter, were missing, the body had been hastily moved from the death scene by rescue staff. An entomology expert was also needed for the estimation of the postmortem interval (PMI). The external examination and the autopsy ruled out external violence, but the decomposition stage prevented any reliable identification of injection marks. Postmortem biochemistry on blood was consistent with the hypothesis of hypoglycemic encephalopathy by insulin overdose with suicidal intent as cause, mode and manner of death. A short review of up to date literature on insulin overdoses is also outlined, with the aim of providing solid scientific references for similar cases.

Learning Overview: After attending this presentation, attendees will understand the effect of carrion insects on fabrics during the postmortem period. In particular, attendees will understand those variables (e.g., the nature of the fabric [natural, synthetic, blended], the fabric’s elastic content [% in elastane], the type of initial damage [tear/penetration] that will most impact on how the fabric modifies during a decomposition event). Impact on the Forensic Science Community: This presentation will impact the forensic science community by providing a new body of information that will enhance the investigative role of clothing associated with decomposed and skeletonized remains. Fatal stabbing incidents are the leading cause of homicides predominantly in countries with restricted access to firearms, such as Australia. During a stabbing assault, the distinctive characteristics of an implement deposit specific features, typically assessed during wound examination by a pathologist and/or during a fabric damage assessment by a forensic scientist. When the decomposition process impedes the identification and evaluation of the type and extent of a stabbing wound, fabric damage analysis on the victim’s clothing may provide information about the implement or the actions that caused the injuries. However, studies have suggested that insect activity can modify the original cut (e.g., exacerbating the fraying of a fabric’s cut, especially the edges of the cut). Furthermore, insect activity and the progression of decomposition such as bloating have also been reported to produce changes to clothing that in some cases may mimic indicators of sexual homicides. At present, there is a paucity of research focused on the effect of insect activity on different fabrics and the modifications they cause throughout the process of decomposition. The aim of this study is to analyze the effects of the activity of the necrophagous entomofauna during a summer season in Western Australia on d

Learning Overview: The goal of this presentation is to describe the principles of bone diagenesis and their potential application to forensic science, the distinction between biogenic and diagenetic chemical signals in bones, the correlation between macroscopic and chemical characteristics of bone diagenesis, and an example of trace element analysis in a set of archaeological bones submerged for a known length of time in a marine environment. Impact on the Forensic Science Community: This presentation will impact the forensic science community by describing how the establishment of a correlation between macroscopic alterations and trace element concentrations and distributions in bones recovered from a marine environment will prove essential for members of the forensic science community involved in taphonomic assessments. Bone diagenesis is the global effect of the physical, biological, and chemical transformations that bones undergo between death and discovery in the archaeological or geological record. Diagenetic transformations, macroscopic and microscopic, are influenced by the physics, chemistry, and biology of the depositional environment. In living organisms, chemical processes are affected by diet, mobility, and pathologies. While studies of the diagenetic modifications and chemical composition of buried bones are extensively featured in the scientific literature, geochemical signatures characteristic of underwater bone diagenesis have not been studied in detail. This study investigated whether a geochemical fingerprint of the interaction between 11 archaeological mammalian bones and seawater and/or marine sediment could be discerned. The analyzed mammalian bones belong to a museum collection of underwater archaeological materials excavated from four submerged shipwreck sites off the Western Australia coast: Batavia (1629), Vergulde Draeck (1656), Zeewijk (1727) and Rapid (1811). The underwater excavations were conducted between 1968 and 1980, and bones from

In the analysis of any forensic case, the estimation of time, cause and manner of death is affected by post-mortem changes. These are inextricably linked to both intrinsic characteristics of the body and a variety of external factors, mainly environmental, such as the presence and types of scavengers. While there are several research and case-studies on terrestrial environments, there is scant knowledge regarding aquatic environments, either stable or cyclical/seasonal. At present, no case studies have considered human remains discovered in the mud, following a flooding event. This case study describes a body discovered in a floodplain area in northern Italy. After a flood event, the water progressively drained out, leaving the body in the mud. Besides the unique conditions of the remains, of particular interest was the colonization by larvae of Calliphora vomitoria (L.) (Diptera: Calliphoridae) and raft spiders, Dolomedes fimbriatus (Clerck) (Araneae: Pisauridae), for the first-time recorded colonizing a corpse. The multidisciplinary approach to such an investigation is described.

Introduction: Singapore is a first-world country, protected by an advanced police force, competent in investigating crime against human citizens. Little is known about crime against animals and what Singapore does in these instances. Methods: This research considered data from reports of various animal crime that occurred in 2016 as recorded by the three animal welfare organizations appointed to investigate crimes against animals (AVS, SPCA and ACRES). Details of the cases collected were sorted using eight parameters: date, location, perpetrator, victim, type of crime or complaint, case outcome, organization, and punishment. The type of crime or complaint were divided into the categories: abandoned, allowed to roam, barking animal, caged, poor conditions, sick and untreated, physically abused, poisoned, deceased, taken from the wild, illegal possession, illegal sale, smuggling, and licencing. Results: In 2016, a total of 831 cases committed by 839 perpetrators were reported, for a total of 2357 minimum number of animals involved, comprehensive of animal parts and items made from animals. The largest proportion of the cases occurred against domestic animals and in housing districts. Despite these high numbers, only perpetrators in 11 cases were charged with fines and/or imprisonment time. Conclusions: This research paints, for the first time, a picture of what animal crime looks in the Singaporean society. Results are of pivotal importance to identify criminal hot spots, most common type of crimes, and animal categories that are most often victimised, aiming to facilitate the work of the organizations involved in investigating animal-related crime in Singapore.

Changes in common and widespread insect populations such as the domestic filth fly in urban cities are useful and relevant bioindicators for overall changes in the insect biomass. The current study surveyed necrophagous flies by placing a weekly trap from June–September over a two-year period in the city of New Haven, Connecticut, to compare data on fly abundance and diversity with data collected 78 years earlier. Climate and land cover changes were also assessed in combination with the fly population for each period. The survey results suggest the domestic filth fly population is now less diverse with decreased species richness and changes in the relative abundance of species. In both surveys, 95–96% of the population was composed of only three species. The current survey data indicate the numerical dominance of Lucilia sericata has decreased, the abundance of several species, notably Lucilia coeruleiviridis, has increased, and Lucilia illustris is absent. Species that showed a significant interaction with temperature in the 1940s survey have now increased in abundance, with several of the trapped species continuing to show an interaction with temperature and rainfall. Analysis of the land cover and climate data characterizes the trap site as a region exposed to a prolonged period of industrialization and urbanization, with only 7% of the land cover remaining undeveloped and over 50% impervious, coupled with an increase in temperature and rainfall. This study serves as a model for changes in domestic filth fly populations and other insects in similarly highly urbanized established cities.

Diagenesis is the collective word for the physical, biological, and chemical processes that bones undergo in the post-mortem period, until their physical destruction or fossilization. In forensic anthropology, the analysis of macroscopic and microscopic bone alterations, alongside the taphonomy of the soft tissues of a body, has proven valuable for the estimation of the time-of-death, or Post-Mortem Interval (PMI), of skeletonized individuals. To date, bone alterations have been mostly researched in terrestrial settings, such as exposed or buried skeletal remains, but here the scientific literature regarding human bones submerged underwater has been reviewed. It features 20 publications in the last 42 years, of which 9 are reviews, 8 are studies on ancient material and 3 are experimental studies. Future research on analysis of microscopic diagenetic parameters of submerged bones, together with the refinement of the correlation with time of the slightly better known macroscopic underwater alterations, will prove valuable for the estimation of a Post-Mortem Submersion Interval (PMSI) in both forensic and archaeological contexts, because bones have always been and still are regularly recovered underwater. The concurrent estimation of both PMI and PMSI of bones recovered underwater will add vital information to criminal investigations. Diagenetic parameters have been identified in Histological Index, protein content, porosity and crystallinity of bioapatite. They are depicted with the analytic techniques currently available to assess their presence and magnitude, and to relate them to the diagenetic processes of bioerosion, abrasion, and encrustation, but also to the extremes of dissolution or fossilization.

After death, the body begins decomposition, a process that starts with the breakdown of organic matter and typically leads to the complete degradation of a body. Such a process is highly affected by (micro and macro) environmental factors of intrinsic and extrinsic nature. Adipocere is a substance formed from the decomposition of adipose tissue and represents a disruption to the typical decomposition process. Such disruption causes decomposition to slow or arrest completely, placing a body into a state of preservation, and causes complications in the estimation of the time since death (Post-Mortem Interval, PMI). While several studies have been performed on the nature, the formation and the degradation of adipocere, there is still no reliable model to assess the PMI of a body exhibiting it. Case studies are an important source to aid pathologists and investigators during a case. This review presents a summary and an update on the knowledge surrounding the chemistry and the factors affecting adipocere formation and degradation, the timing and the distribution of adipocere throughout a body, and the techniques used to investigate it. Furthermore, a table of the most important case studies involving adipocere since 1950, several images and descriptions of recent cases and operational considerations for the best practice at the crime scene and autopsy are presented to be used as a reference to facilitate forensic professionals in adipocere cases.

The Coronavirus 2019 (COVID-19) pandemic has created significant challenges for the global higher education community. Through a desktop analysis leveraging university and government sources where possible, we provide a timely map of the intra-period higher education responses to COVID-19 across 20 countries. We found that the responses by higher education providers have been diverse from having no response through to social isolation strategies on campus and rapid curriculum redevelopment for fully online offerings. We provide in our discussion a typology of the types of responses currently undertaken and assess the agility of higher education in preparing for the pandemic. We believe there are significant opportunities to learn from the pedagogical developments of other universities, in order to strengthen our collective response to COVID-19 now and into the future.

Crime scene investigation protocols and procedures are well established when concerning terrestrial environments, but are limited when associated with aquatic environments. In aquatic contexts, the soil, sediments and microscopic algae (e.g. diatoms) may all be used as sources of trace evidence for criminal investigations. Diatoms are one such source that can be used to support the diagnosis of death by drowning and can be used to verify contact between a suspect and a specific water body. For the latter, diatoms can be collected from clothing and shoes for forensic comparisons. Over the years, there have been several methods proposed for the extraction of diatoms from clothing, however, a best practice method is yet to be established and is still open to debate. The present research represents the first investigation that evaluates four different methods for the extraction of native and common Australian diatom species from four different types of fabrics. Diatoms of two different species and shape (pennate and centric) were cultivated in stimulated experimental waters – prepared using either monocultures or mixed cultures of the selected diatoms. Diatom concentrations were set to mimic the natural population (low to bloom events) as commonly found in the Swan River Estuary of Western Australia. Cotton, denim, blend, and acrylic fabric types were placed in experimental waters. Diatom extraction was attempted using methods already proposed in the literature, these include: rinsing with ethanol (RE); rinsing with ethanol together with the application of centrifugal force (RECF); digestion with hydrogen peroxide (H); and a new method of ashing that has never been tested before (dry ash, DA). Results of this research showed that a) the type and the concentration of the mono and mix diatom mixtures significantly affects the transfer and extraction from different types of fabric; b) a noticeable extraction difference is observed between the fabrics, establishing denim, a

Twelve large mammal bones, associated with underwater archaeological excavations on four shipwrecks on the Western Australia coast, were analysed by Micro-CT Scan (Skyscan 1176). They all belong to the collections of the Shipwrecks Museum, a section of the Western Australian Museum in Fremantle, Western Australia. The aim is the investigation of any physical modifications linked to underwater taphonomy and diagenesis of bone.

The estimation of the time since death (minimum Post Mortem Interval, minPMI) is an essential aspect to a forensic investigation, and this is particularly complex when a body is found in a marine environment, submerged, floating or beached. The estimation of the minPMI of a body found on land is generally based on the presence of carrion insects (Diptera: Calliphoridae; Sarcophagidae; Muscidae). In the marine environment, beside the minPMI, the time spent by the body underwater (minPMSI, minimum Post Mortem Submersion Interval) or floating (FI, Floating Interval) are necessary for a correct crime scene reconstruction. The presence of barnacles (Crustacea: Cirripedia) is one parameter that has come under recent scrutiny because of their colonisation and permanent settlement on human remains and their accompanying items, such as clothing and shoes. However, research in this field to present day is limited and focused in USA and Europe, and has not considered human clothing materials (fabrics). This research is focused on the colonisation – settlement preference and growth – of barnacles on cotton, velvet, satin and neoprene. Polystyrene floats (PFs), covered by the four types of fabric, were submerged over a period of six months off the coast of Perth, Western Australia. The aims of this research were 1) the identification of marine species colonising the fabrics, with special attention to barnacles; 2) the identification of which fabric provides the most desirable environment for colonisation; and 3) the identification of which factors affect the growth rate of the different species. Three species of barnacles (Balanus trigonus Darwin, Amphibalanus reticulatus (Utinomi) and A. variegatus (Darwin)) were present in varying amounts and sizes. The colonisation process of the barnacles happened rapidly, with colonisation observed in within the first month on neoprene. Statistical analyses were used to determine statically significant relationships between the barnacles’ n

Wingbeat frequency and harmonics are being used to successfully identify agricultural pests and disease vectors in the field. This method is a viable alternative for identifying the forensically important blow fly (Diptera: Calliphoridae). The use of morphometric features and DNA to identify blow flies is difficult, time consuming and expensive, and are complicated by changes in species distribution and the appearance of invasive and hybrid species. Blow flies are the first to colonise decomposing remains and are ubiquitous. They provide valuable evidence when calculating time since death, as the development of the blow fly is highly predictable when using temperature and experimental reference data for the correctly identified species. However, species misidentification results in significant errors in estimating the time since death, as different blow fly species often have different developmental times and life history traits. Wingbeat frequency is measured using an optical sensor to record light fluctuations produced by the wings of an insect partially blocking the light when it flies between a laser beam and a phototransistor array. These fluctuations are then analysed by a classification model built and trained using a machine learning algorithm to identify each specimen by species and sex. This method enables the unbiased identification of an adult blow fly species with a reportable potential rate of error. The adult blow fly is the most mobile stage of the life cycle, determining the species’ presence in a decomposing community, and the speed at which it arrives. However, its role has been largely overlooked with much of the research being focused on the larvae. Flying adult flies are also difficult to study due to their small size and speed. The use of wingbeat frequency data to track and identify blowflies in real time will enable the research of arrival patterns of adult blow flies and the factors that influence detection and acceptance of a site for ovip

People of different cultures bury their dead in different ways, based on religious beliefs, historical rituals, or public health requirements. In Italy, cremation is still a limited practice compared to entombment and inhumation. Accordingly with the law, a buried body can be moved to the cemetery ossuary only if skeletonized. Generally, complete skeletonization occurs within 40 years following burial, but sometimes the body may mummify, or it may turn into adipocere. Globally, today burial space is limited with cemeteries facing a growing need for both burials and entombments. The present study considered the thanatological, taphonomical, anthropological, microbiological and geochemical examination of 408 human bodies exhumed from grave pits and stone tombs located in two cemeteries in Parma, Italy. Intrinsic and extrinsic factors associated with the process of the decomposition of such bodies were documented in order to identify which factors promote or reduce the time needed for skeletonization. Overall, the aim of this study was to improve the management of the body turnover in cemeteries, providing recommendations for cemetery management and turnover planning, with the goal of avoiding extra costs that may be attributed to the family and the State. The results of this study show that inhumation in burial grounds and soils with a high percentage of sand and gravel promote decomposition and skeletonization, whereas entombments inside stone tombs and the presence of stone slabs on the ground covering burials, aid in the formation of adipocere, slowing down the decomposition process.

Decomposition is a complex and continuous process that involves the breakdown of soft tissues following a death event. This is often mediated by the action of micro- and macro-fauna, especially necrophagous insects belonging to the Orders Diptera and Coleoptera. Access to the cadaver is a major prerequisite for decomposition to proceed. However, a cadaver maybe concealed to avoid an easy discovery by the authorities. The present field study investigated the decomposition process of a carcass placed in a suitcase in Western Australia. A total of six pig carcasses (Sus scrofa L.) were used as a substitute for human cadavers. Five pigs were singularly placed in identical zip suitcases made of fabric and a sixth pig being fully exposed (control pig). As expected, the study showed that the rate of decomposition differed between the control pig and concealed pigs. The rate of decomposition of the control pig followed the typical pattern, in terms of stages of decomposition, and revealed the insect species involved in the process. Pigs inside the suitcases exhibited the characteristics of wet decomposition. This occurred despite no significant differences between the ambient temperature and the temperature inside the suitcases. However, the relative humidity inside of the suitcases was found to be statistically different and the pattern of insect succession varied between the concealed carcasses and the control pig. Carcass attendance by blow flies at the control occurred within minutes of positioning, and oviposition occurred within the first day. In contrast, blow flies were not observed visiting the suitcases until the next day, with oviposition on the external part of the suitcases not recorded until day 9 and inside the suitcases after day 14. The insect species also varied between those found inside the suitcases and on the control, with families Phoridae (scuttle flies) and Fanniidae (lesser house flies) being the most prevalent species inside the suitcases. This pi

Insect succession has been studied around the world using the predictable and mostly sequential arrival pattern of different insect species that are attracted to a decomposing carcass. In cases of suspicious death of humans and animals, carrion insects may be used to assist in crime scene reconstruction. The present research represents the first study in forensic entomology to be undertaken in Tasmania, investigating insect succession patterns on decomposing pig carcasses and providing a preliminary database of forensically important insects. Six pig carcasses were placed in two contrasting locations (rural and urban) in northern Tasmania. Insect successional waves were recorded over a 40-day study during the austral summer season. Results showed that decomposition rates and insect assemblages varied between each location. Eleven insect taxa, representing nine families, were identified in association with the decomposition of the pig carcasses at both localities. Blowflies present on the pig carcasses throughout the decomposition process were Calliphora stygia Malloch (Diptera: Calliphoridae) at both sites and Lucilia sericata (Meigen) at the urban site only. These preliminary results will provide useful information in any future casework involving human remains and associated insect material in Tasmania.

Forensic science is the application of science in a criminal investigation or court of law. Crimes and forensic investigation have always been associated with human society. As an interdisciplinary field, forensic science incorporates areas of both life sciences and social sciences. Until the beginning of the 1900s, forensic science was self-taught and carried out exclusively through law enforcement agencies. In recent years, with the success of crime drama television series, an increased global interest in forensic science-based university courses has been observed. The prevalence of global issues such as international crimes and mass disasters has catalysed the need for international collaboration and to remove global barriers within forensic science. To prepare students for the world of work, it is pivotal that forensic science curricula address the needs of the present-day society and the requirements of the forensic providers (laboratories and law enforcement agencies). The present manuscript describes the first forensic science international curriculum between Australia (Murdoch University) and Malaysia (Universiti Kebangsaan Malaysia/UKM Forensics), possible through the New Colombo Plan of the Australian Government. The course outlined here, comprehensively comprised of lectures, workshops and mixed group crime scene simulations, provided Australian and Malaysian students with an authentic, intercultural and cross-jurisdictional learning experience to acquire work-ready skills.

Chrysomya megacephala Fabricius is one of the most common myiasis-causing and carrion-feeding fly species in the tropics. This species has a worldwide distribution due to globalization and therefore is of critical importance to the public health sector and forensic investigations. Development studies carried worldwide show that Ch. megacephala reared at similar temperatures may require different amounts of time to complete their cycle of growth. The present study determined for the first time the life-history information of this species in Sri Lanka. Chrysomya megacephala colonies were reared on three different food sources (bovine muscle, swine muscle, and swine liver) considering the most common forensic entomology rearing mediums and the capability of Ch. megacephala to cause myiasis in cattle. Furthermore, colonies were reared at four temperature regimes representing several specific development conditions of this blowfly: 1) 20°C representing the estimation of the lower developmental threshold for this species in Sri Lanka; 2) 25 and 27°C representing typical room/environmental temperatures in Sri Lanka; and 3) 38°C as this is the typical living animal temperature, representing myiasis conditions. Results show that temperature significantly affected larval length and width over time; however, tissue type was not a significant factor. The fastest development was recorded at 38°C for immature feeding on bovine muscle (162.15 h), followed by those fed swine liver (184.15 h) and swine muscle (208.00 h). The calculated minimum temperature threshold for Ch. megacephala was 13°C. Data generated will be crucial for future forensic investigations involving living or deceased individuals colonized by this species.

Entomotoxicology studies employ analytical methods and instrumentation to detect chemical substances in carrion insects feeding from the decomposing tissues. The identification of such chemicals may determine the cause of death and may be used for the estimation of the minimum time since death. To date, the main focus of entomotoxicological studies has been the detection of drugs, whereas little information concerns the effects of pesticides on blowflies. Pesticides are generally freely available and more affordable than drugs but they can also be a home hazard and an accessible candidate poison at a crime scene. A QuEChERS extraction method followed by Gas chromatography–mass spectrometry (GC-MS) analysis was developed for the detection of α- and β-endosulfan (organochlorine insecticide and acaricide) in Calliphora vomitoria L. (Diptera: Calliphoridae) and validated. Furthermore, the effects of endosulfan on the morphology, development time and survival of the immature blowflies were investigated. Larvae were reared on liver substrates homogeneously spiked with aliquots of endosulfan corresponding to the concentrations found in body tissues of humans and animals involved in endosulfan poisoning. Results demonstrated that the combination of QuEChERS extraction and GC-MS provide an adequate methods to detect both α- and β-endosulfan in blowfly immatures. Furthermore, the presence of α- and β-endosulfan in the food source 1) prevented C. vomitoria immatures reaching the pupal instar and, therefore, the adult instar at high concentrations, 2) did not affect the developmental time of blowflies at low concentrations 3) affected the size of immatures only at high concentrations, resulting in significantly smaller larvae.

Estimating the time since death (minimum Post Mortem Interval, minPMI) is a necessary part of a forensic investigation. Besides considering the typical signs of death, minPMI can be estimated using the insects and other arthropods that colonize the remains (forensic entomology). In an aquatic environment, both insects and crustaceans may provide information regarding the time spent in water of the remains (minimum Floating Interval, minFI and minimum Post Mortem Submersion Interval, minPMSI), and this can also assist in determining the minPMI. Barnacles (Crustacea: Cirripedia) are common crustaceans that colonize solid substrates in marine environments and they can be found in association with organic and inorganic remains recovered from the sea. Barnacles colonize both floating and submerged remains and their growth rate is temperature dependent. Despite their potential to be indicative of the minFI and/or minPMSI, only a few case studies have considered it for this purpose, and scant research has been conducted in this field. Assuming that the vast majority of the bodies found in the sea are clothed, this research is focused on the barnacle colonization of two different types of shoes placed in the sea, in order to 1) identify the colonizing species in the chosen environment; 2) identify the settlement preferences of the barnacles associated with the shoes; and 3) determine the factors affecting the growth rate of the barnacles associated with the shoes. In April 2016 64 sport shoes (SS) and 64 patent leather shoes (PLS) were placed in the Boston Harbor (MA-USA) at 8/10 meters below sea level. Four of each shoe type were collected every two weeks for seven months. Individual barnacles from each shoe were sampled and measured to determine species and age. The overall colonization density and settlement preference was statistically analyzed. Results show that a) Amphibalanus improvisus (Darwin) (Crustacea: Cirripedia: Sessilia) colonized the vast majority of shoes

Entomotoxicology is a branch of forensic entomology that studies the detection of drugs or other toxic substances from insects developing on the decomposing tissues of a human corpse or animal carcass Entomotoxicology also investigates the effects of these substances on insect development, survival and morphology to provide an estimation of the minimum time since death. Ketamine is a medication mainly used for starting and maintaining anesthesia. In recent years ketamine has also been used as a recreational drug, and occasionally as a sedating drug to facilitate sexual assault. In both activities, it has resulted in several deaths. Furthermore, ketamine has been also implicated in suspicious deaths of animals. The present research describes for the first time the development and validation of an analytical method suited to detect ketamine in larvae, pupae, empty puparia, and adults of Calliphora vomitoria L. (Diptera: Calliphoridae), using liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This research also considers the effects of ketamine on the survival, developmental rate and morphology (length and width of larvae and pupae) of C. vomitoria. The larvae were reared on liver substrates homogeneously spiked with ketamine concentrations consistent with those found in humans after recreational use (300 ng/mg) or allegedly indicated as capable of causing death in either humans or animals (600 ng/mg). The results demonstrated that (a) HPLC-MS/MS method is applicable to ketamine detection in C. vomitoria immatures, not adults; (b) the presence of ketamine at either concentration in the food substrate significantly delays the developmental time to pupal and adult instar; (d) the survival of C. vomitoria is negatively affected by the presence of ketamine in the substrate; (e) the length and width of larvae and pupae exposed to either ketamine concentration were significantly larger than the control samples.