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Microautoradiography was used to study photosynthetic and heterotrophic activities in cells of the diatom Rhizosolenia styliformis var. longispina containing the nitrogen-fixing, endophytic, blue-green alga Richelia intracellularis. In field studies with [sup14]C-labeled bicarbonate, the blue-green alga appeared to be actively photosynthesizing but the diatom did not. Neither Richelia nor Rhizosolenia assimilated [sup3]H-labeled acetate, glucose, or an amino acid mixture. The diatom lacked cytoplasmic streaming and appeared to be in a senescent condition. Implications of these observations for the nature of this algal association are discussed. [ABSTRACT FROM AUTHOR]

Fifty amino acids are analyzed as their N-heptafluorobutyryl isobutyl esters by gas chromatography on a column of 3% OV-101 on Gas-Chrom Q. The identification and quantification of unusual amino acids, specifically those occurring in the marine environment, in the presence of common protein amino acids is reported. Regular relationships between elution temperatures and amino acid structures are found, thus allowing the estimation of elution temperatures of other amino acids.