Search

Your search keyword '"Mahnke, Donna"' showing total 27 results
Start Over Author "Mahnke, Donna"
27 results on '"Mahnke, Donna"'

5. Total Cell-Free DNA Predicts Death and Infection Following Pediatric and Adult Heart Transplantation

Author

Scott, John P., primary, Ragalie, William S., additional, Stamm, Karl D., additional, Mahnke, Donna K., additional, Liang, Huan ling, additional, Simpson, Pippa M., additional, Dasgupta, Mahua, additional, Katz, Ronit, additional, North, Paula E., additional, Tomita-Mitchell, Aoy, additional, Zangwill, Steven D., additional, Kindel, Steven J., additional, and Mitchell, Michael E., additional

Published
2021
Full Text
View/download PDF

6. Calcium activates erythrocyte AMP deaminase [isoform E (AMPD3)] through a protein-protein interaction between calmodulin and the N-terminal domain of the AMPD3 polypeptide

Author

Sabina, Richard L. and Mahnke, Donna K.

Subjects
Calmodulin -- Research, Proteins -- Research, Calcium compounds -- Research, Adenylic acid -- Research, Biological sciences, Chemistry
Abstract

Ca(super 2+) regulation of erythrocyte AMP deaminase [isoform E (AMPD3)] was studied by examining in vitro effects of calmodulin (CaM) on the enzyme and by monitoring the influence of CaM antagonists on purine catabolic flow in freshly prepared erythrocytes under various conditions of energy imbalance. The results suggested a protein-protein interaction between Ca(super 2+) -CaM and isoform E.

Published
2005

7. Cover

Author

Samudrala, Sai Suma K., primary, North, Lauren M., additional, Stamm, Karl D., additional, Earing, Michael G., additional, Frommelt, Michele A., additional, Willes, Richard, additional, Tripathi, Swarnendu, additional, Dsouza, Nikita R., additional, Zimmermann, Michael T., additional, Mahnke, Donna K., additional, Liang, Huan Ling, additional, Lund, Michael, additional, Lin, Chien‐Wei, additional, Geddes, Gabrielle C., additional, Mitchell, Michael E., additional, and Tomita‐Mitchell, Aoy, additional

Published
2020
Full Text
View/download PDF

8. Novel KLHL26 variant associated with a familial case of Ebstein’s anomaly and left ventricular noncompaction

Author

Samudrala, Sai Suma K., primary, North, Lauren M., additional, Stamm, Karl D., additional, Earing, Michael G., additional, Frommelt, Michele A., additional, Willes, Richard, additional, Tripathi, Swarnendu, additional, Dsouza, Nikita R., additional, Zimmermann, Michael T., additional, Mahnke, Donna K., additional, Liang, Huan Ling, additional, Lund, Michael, additional, Lin, Chien‐Wei, additional, Geddes, Gabrielle C., additional, Mitchell, Michael E., additional, and Tomita‐Mitchell, Aoy, additional

Published
2020
Full Text
View/download PDF

9. Cell-free DNA donor fraction analysis in pediatric and adult heart transplant patients by multiplexed allele-specific quantitative PCR: Validation of a rapid and highly sensitive clinical test for stratification of rejection probability

Author

North, Paula E., primary, Ziegler, Emily, additional, Mahnke, Donna K., additional, Stamm, Karl D., additional, Thomm, Angela, additional, Daft, Paul, additional, Goetsch, Mary, additional, Liang, Huan ling, additional, Baker, Maria Angeles, additional, Vepraskas, Adam, additional, Rosenau, Chris, additional, Dasgupta, Mahua, additional, Simpson, Pippa, additional, Mitchell, Michael E., additional, and Tomita-Mitchell, Aoy, additional

Published
2020
Full Text
View/download PDF

10. Human genotyping and an experimental model reveal NPR-C as a possible contributor to morbidity in coarctation of the aorta

Author

LaDisa, John F., primary, Tomita-Mitchell, Aoy, additional, Stamm, Karl, additional, Bazan, Kathleen, additional, Mahnke, Donna K., additional, Goetsch, Mary A., additional, Wegter, Brandon J., additional, Gerringer, Jesse W., additional, Repp, Kathryn, additional, Palygin, Oleg, additional, Zietara, Adrian P., additional, Krolikowski, Mary M., additional, Eddinger, Thomas J., additional, Alli, Abdel A., additional, and Mitchell, Michael E., additional

Published
2019
Full Text
View/download PDF

12. NOVEL ASSAY TO CALCULATE DONOR FRACTION OF CELL-FREE DNA IN HEART TRANSPLANTATION

Author

Huan Ling Liang, Pippa Simpson, Pip M. Hidestrand, Mats Hidestrand, Steven J. Kindel, Steven Zangwill, Ronit Katz, Michael E. Mitchell, William S. Ragalie, Mary Goetsch, Karl Stamm, Gail Stendahl, Mahnke Donna, and Aoy Tomita-Mitchell

Subjects
Heart transplantation, medicine.medical_specialty, business.industry, medicine.medical_treatment, Urology, Fraction (chemistry), 030204 cardiovascular system & hematology, Turnaround time, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, 030228 respiratory system, Cell-free fetal DNA, chemistry, Medicine, Cardiology and Cardiovascular Medicine, business, Throughput (business), DNA, Blinded study
Abstract

Donor fraction (DF) of cell-free DNA (cf-DNA) has been proposed as a noninvasive surveillance method in heart transplantation. We present our pilot experience with a novel assay to quantify DF (%) with a clinically practical throughput and turnaround time. A blinded study was performed in which

Published
2018

14. Impact of MYH6 variants in hypoplastic left heart syndrome

Author

Tomita-Mitchell, Aoy, primary, Stamm, Karl D., additional, Mahnke, Donna K., additional, Kim, Min-Su, additional, Hidestrand, Pip M., additional, Liang, Huan Ling, additional, Goetsch, Mary A., additional, Hidestrand, Mats, additional, Simpson, Pippa, additional, Pelech, Andrew N., additional, Tweddell, James S., additional, Benson, D. Woodrow, additional, Lough, John W., additional, and Mitchell, Michael E., additional

Published
2016
Full Text
View/download PDF

17. Human gene copy number spectra analysis in congenital heart malformations

Author

Tomita-Mitchell, Aoy, primary, Mahnke, Donna K., additional, Struble, Craig A., additional, Tuffnell, Maureen E., additional, Stamm, Karl D., additional, Hidestrand, Mats, additional, Harris, Susan E., additional, Goetsch, Mary A., additional, Simpson, Pippa M., additional, Bick, David P., additional, Broeckel, Ulrich, additional, Pelech, Andrew N., additional, Tweddell, James S., additional, and Mitchell, Michael E., additional

Published
2012
Full Text
View/download PDF

18. Multiplexed quantitative real-time PCR to detect 22q11.2 deletion in patients with congenital heart disease

Author

Tomita-Mitchell, Aoy, primary, Mahnke, Donna K., additional, Larson, Joshua M., additional, Ghanta, Sujana, additional, Feng, Ying, additional, Simpson, Pippa M., additional, Broeckel, Ulrich, additional, Duffy, Kelly, additional, Tweddell, James S., additional, Grossman, William J., additional, Routes, John M., additional, and Mitchell, Michael E., additional

Published
2010
Full Text
View/download PDF

22. Effect of 22q11.2 deletion on bleeding and transfusion utilization in children with congenital heart disease undergoing cardiac surgery

Author

Brenner, Michelle K., Clarke, Shanelle, Mahnke, Donna K., Simpson, Pippa, Bercovitz, Rachel S., Tomita-Mitchell, Aoy, Mitchell, Michael E., and Newman, Debra K.

Abstract

Background:Postsurgical bleeding causes significant morbidity and mortality in children undergoing surgery for congenital heart defects (CHD). 22q11.2 deletion syndrome (DS) is the second most common genetic risk factor for CHD. The deleted segment of chromosome 22q11.2 encompasses the gene encoding glycoprotein (GP) Ibß, which is required for expression of the GPIb-V-IX complex on the platelet surface, where it functions as the receptor for von Willebrand factor (VWF). Binding of GPIb-V-IX to VWF is important for platelets to initiate hemostasis. It is not known whether hemizygosity for the gene encoding GPIbß increases the risk for bleeding following cardiac surgery for patients with 22q11.2 DS.Methods:We performed a case–control study of 91 pediatric patients who underwent cardiac surgery with cardiopulmonary bypass from 2004 to 2012 at Children’s Hospital of Wisconsin.Results:Patients with 22q11.2 DS had larger platelets and lower platelet counts, bled more excessively, and received more transfusion support with packed red blood cells in the early postoperative period relative to control patients.Conclusion:Presurgical genetic testing for 22q11.2 DS may help to identify a subset of pediatric cardiac surgery patients who are at increased risk for excessive bleeding and who may require more transfusion support in the postoperative period.

Published
2016
Full Text
View/download PDF

24. Calcium Activates Erythrocyte AMP Deaminase [Isoform E (AMPD3)] through a Protein-Protein Interaction between Calmodulin and the N-Terminal Domain of the AMPD 3 Polypeptide.

Author

Mahnke, Donna K. and Sabina, Richard L.

Subjects
*ERYTHROCYTES, *CALCIUM, *CALMODULIN, *ORGANIC compounds, *ADENINE nucleotides, *CATIONS
Abstract

Erythrocyte AMP deaminase [isoform E (AMPD3)] is activated in response to increased intracellular calcium levels in Tarui's disease, following exposure of ionophore-treated cells to extracellular calcium, and by the addition of calcium to freshly prepared hemolysates. However, the assumption that Ca2+ is a positive effector of isoform E is inconsistent with the loss of sensitivity to this divalent cation following dilution of erythrocyte lysates or enzyme purification. Ca2+ regulation of isoform E was studied by examining in vitro effects of calmodulin (CaM) on this enzyme and by monitoring the influence of CaM antagonists on purine catabolic flow in freshly prepared erythrocytes under various conditions of energy imbalance. Erythrocyte and recombinant isoform E both adsorb to immobilized Ca2+-.CaM, and relative adsorption across a series of N-truncated recombinant enzymes localizes CaM binding determinants to within residues 65-89 of the AMPD3 polypeptide. Ca2+-CaM directly stimulates isoform B catalytic activity through a Kmapp effect and also antagonizes the protein-lipid interaction between this enzyme and intracellular membranes that inhibits catalytic activity. AMP is the predominant purine catabolite in erythrocytes deprived of glucose or exposed to A23187 ionophore alone, whereas IMP accumulates when Ca2+ is included under the latter conditions and also during autoincubation at 37 °C. Preincubation with a CaM antagonist significantly slows the accumulation of erythrocyte IMP under both conditions. The combined results reveal a protein-protein interaction between Ca2+-CaM and isoform E and identify a mechanism that advances our understanding of erythrocyte purine metabolism. Ca2+-CaM overcomes potent isoform B inhibitory mechanisms that function to maintain the total adenine nucleotide pool in mature erythrocytes, which are unable to synthesize AMP from IMP because of a developmental loss of adenylosuccinate synthetase. This may also explain why Tarui's disease erythrocytes exhibit accelerated adenine nucleotide depletion in response to an increase in intracellular Ca2+ concentration. This regulatory mechanism could also play an important role in purine metabolism in other human tissues and cells where the AMPD3 gene is expressed. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

26. Crystallization and preliminary X-ray crystallographic analysis of adenosine 5'-monophosphate deaminase (AMPD) from Arabidopsis thaliana in complex with coformycin 5'-phosphate.

Author

Han, Byung Woo, Bingman, Craig A., Mahnke, Donna K., Sabina, Richard L., and Phillips, Jr, George N.

Subjects
ARABIDOPSIS, CRYSTALLIZATION, X-rays, CRYSTALS, BRASSICACEAE, ADENOSINES
Abstract

Adenosine 5'-monophosphate deaminase (AMPD) is a eukaryotic enzyme that converts adenosine 5'-monophosphate (AMP) to inosine 5'-monophosphate (IMP) and ammonia. AMPD from Arabidopsis thaliana (AtAMPD) was cloned into the baculoviral transfer vector p2Bac and co-transfected along with a modified baculoviral genome into Spodoptera frugiperda (Sf9) cells. The resulting recombinant baculovirus were plaque-purified, amplified and used to overexpress recombinant AtAMPD. Crystals of purified AtAMPD have been obtained to which coformycin 5'-phosphate, a transition-state inhibitor, is bound. Crystals belong to space group P6222, with unit-cell parameters a = b = 131.325, c = 208.254 Å , α = β 90, γ = 120°. Diffraction data were collected to 3.34 Å resolution from a crystal in complex with coformycin 5'-phosphate and to 4.05 Å resolution from a crystal of a mercury derivative. [ABSTRACT FROM AUTHOR]

Published
2005
Full Text
View/download PDF

27. Membrane Association, Mechanism of Action, and Structure of Arabidopsis Embryonic Factor 1 (FAC1).

Author

Byung Woo Han, Bingman, Craig A., Mahnke, Donna K., Bannen, Ryan M., Bednarek, Sebastian Y., Sabina, Richard L., and Phillips Jr., George N.

Subjects
*ARABIDOPSIS, *SUBCELLULAR fractionation, *ADENOSINE monophosphate, *ADENOSINE deaminase, *X-ray crystallography, *ADENOSINE triphosphate, *DIMERS, *HERBICIDES
Abstract

Embryonic factor 1 (FAC1) is one of the earliest expressed plant genes and encodes an AMP deaminase (AMPD), which is also an identified herbicide target. This report identifies an N-terminal transmembrane domain in Arabidopsis FAC1, explores subcellular fractionation, and presents a 3.3-A globular catalytic domain x-ray crystal structure with a bound herbicide-based transition state inhibitor that provides the first glimpse of a complete AMPD active site. FAC1 contains an (α/β)8-barrel characterized by loops in place of strands 5 and 6 that places it in a small subset of the amidohydrolase superfamily with imperfect folds. Unlike tetrameric animal orthologs, FAC1 is a dimer and each subunit contains an exposed Walker A motif that may be involved in the dramatic combined Km (25–80-fold lower) and Vmax (5–6-fold higher) activation by ATP. Normal mode analysis predicts a hinge motion that flattens basic surfaces on each monomer that flank the dimer interface, which suggests a reversible association between the FAC1 globular catalytic domain and intracellular membranes, with N-terminal transmembrane and disordered linker regions serving as the anchor and attachment to the globular catalytic domain, respectively. [ABSTRACT FROM AUTHOR]

Published
2006
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results