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Background: Ethnopharmacological relevance: Derris brevipes (benth.) Baker is widely used as an anti-arthritic agent in traditional medicine. Aims and Objectives: This study evaluated the anti-inflammatory activity of ethanolic extract of D. brevipes (EEDB) leaves, a member of the Leguminosae family in vitro and ex vivo. Materials and Methods: The EEDB leaves of was screened for anti-inflammatory activities by protein denaturation analysis and proteinase inhibition analysis. Prednisolone was used as the standard. EEDB (at doses of 300 mg/kg and 600 mg/kg) was administered through the oral route. Ex vivo analysis was performed by estimating the expression of tumor necrosis factor-alpha (TNF-a) in collagen (chicken sternal collagen)-induced arthritis (CIA) in Sprague-Dawley rats using the reverse transcription polymerase chain reaction method. The data were analyzed using one-way analysis of variance, followed by Dunnet's multiple comparison tests. Results: Prednisolone (100 mcg/mL) and EEDB (600 mcg/mL) showed percentage inhibition of 80.32% and 80.04%, respectively, in protein denaturation assay. Similarly, prednisolone (600 mcg/mL) and EEDB (600 mcg/mL) showed 66.03% and 56.69% inhibition in the proteinase activity. Synovial tissue TNF-a levels showed a significant decrease in EEDB-treated groups and prednisolone-treated group compared to CIA control rats. Conclusion: From this study, it was concluded that the D. brevipes leaf extract possesses anti-inflammatory activity, which explains its beneficial role in rheumatoid arthritis. [ABSTRACT FROM AUTHOR]

Background: Derris brevipes is a common medicinal plant used in the traditional system of medicine as an anti-arthritic agent. Aims and Objectives: The present study was to evaluate the anti-arthritic activity of ethanolic extract of D. brevipes (EEDB) leaves, belonging to the family Leguminosae. Materials and Methods: The dried leaves were collected and extracted using 95% ethanol, and the extract was subjected to a preliminary phytochemical screening. In vivo activities were evaluated in collagen (chicken sternal collagen)-induced arthritis (CIA) in Sprague–Dawley rats. Prednisolone was used as the standard, and EEDB (at doses of 300 mg/kg and 600 mg/kg) was administered through the oral route. Body weight measurement, arthritic score, histological score, and radiology score assessments were carried out. Results: Preliminary phytochemical screening of leaf extract from D. brevipes showed the presence of components such as steroids, triterpenoids, and flavonoids. In vivo tests on CIA rats given the extract showed that it could lower the arthritic score, the paw volume, the radiological score, and the histological score. Conclusion: From this study, it is concluded that the D. brevipes leaf extract possesses antiarthritic activity. [ABSTRACT FROM AUTHOR]