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246 results on '"Malherbe, Stephanus"'

2. Classification of early tuberculosis states to guide research for improved care and prevention: an international Delphi consensus exercise

Author

Alland, David, Behr, Marcel A, Beko, Busisiwe B, Burhan, Erlina, Churchyard, Gavin, Cobelens, Frank, Denholm, Justin T, Dinkele, Ryan, Ellner, Jerrold J, Fatima, Razia, Haigh, Kate A, Hatherill, Mark, Horton, Katherine C, Kendall, Emily A, Khan, Palwasha Y, MacPherson, Peter, Malherbe, Stephanus T, Mave, Vidya, Mendelsohn, Simon C, Musvosvi, Munyaradzi, Nemes, Elisa, Penn-Nicholson, Adam, Ramamurthy, Dharanidharan, Rangaka, Molebogeng X, Sahu, Suvanand, Schwalb, Alvaro, Shah, Divya K, Sheerin, Dylan, Simon, Donald, Steyn, Adrie J C, Thu Anh, Nguyen, Walzl, Gerhard, Weller, Charlotte L, Williams, Caroline ML, Wong, Emily B, Wood, Robin, Xie, Yingda L, Yi, Siyan, Coussens, Anna K, Zaidi, Syed M A, Allwood, Brian W, Dewan, Puneet K, Gray, Glenda, Kohli, Mikashmi, Kredo, Tamara, Marais, Ben J, Marks, Guy B, Martinez, Leo, Ruhwald, Morten, Scriba, Thomas J, Seddon, James A, Tisile, Phumeza, Warner, Digby F, Wilkinson, Robert J, Esmail, Hanif, and Houben, Rein M G J

Published
2024
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3. Field evaluation of a point-of-care triage test for active tuberculosis (TriageTB)

Author

Richardson, Tracy R., Smith, Bronwyn, Malherbe, Stephanus T., Shaw, Jane Alexandra, Noor, Firdows, MacDonald, Candice, van der Spuy, Gian D., Stanley, Kim, Carstens, Alida, Fisher, Tarryn-Lee, van Rensburg, Ilana, Flinn, Marika, Snyders, Candice, Johnson, Isaac, Fransman, Bernadine, Dockrell, Hazel, Thwaites, Guy, Thuong, Nguyen Thuy Thuong, Schacht, Claudia, Mayanja-Kizza, Harriet, Nsereko, Mary, Tjon Kon Fat, Elisa M., Corstjens, Paul L.A.M., Geluk, Annemieke, Ruhwald, Morton, Penn-Nicholson, Adam, Chegou, Novel N., Sutherland, Jayne, and Walzl, Gerhard

Published
2023
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4. Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research

Author

Shaw, Jane Alexandra, Meiring, Maynard, Allies, Devon, Cruywagen, Lauren, Fisher, Tarryn-Lee, Kasavan, Kesheera, Roos, Kelly, Botha, Stefan Marc, MacDonald, Candice, Hiemstra, Andriёtte M., Simon, Donald, van Rensburg, Ilana, Flinn, Marika, Shabangu, Ayanda, Kuivaniemi, Helena, Tromp, Gerard, Malherbe, Stephanus T., Walzl, Gerhard, and du Plessis, Nelita

Published
2023
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5. Point-of-care C-reactive protein and Xpert MTB/RIF Ultra for tuberculosis screening and diagnosis in unselected antiretroviral therapy initiators: a prospective, cross-sectional, diagnostic accuracy study

Author

Reeve, Byron W P, Ndlangalavu, Gcobisa, Mishra, Hridesh, Palmer, Zaida, Tshivhula, Happy, Rockman, Loren, Naidoo, Selisha, Mbu, Desiree L, Naidoo, Charissa C, Derendinger, Brigitta, Walzl, Gerhard, Malherbe, Stephanus T, van Helden, Paul D, Semitala, Fred C, Yoon, Christina, Gupta, Rishi K, Noursadeghi, Mahdad, Warren, Robin M, and Theron, Grant

Published
2024
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6. Neutrophil degranulation, NETosis and platelet degranulation pathway genes are co-induced in whole blood up to six months before tuberculosis diagnosis

Author

Meier, Stuart, Seddon, James A, Maasdorp, Elizna, Kleynhans, Léanie, du Plessis, Nelita, Loxton, Andre G, Malherbe, Stephanus T, Zak, Daniel E, Thompson, Ethan, Duffy, Fergal J, Kaufmann, Stefan HE, Ottenhoff, Tom HM, Scriba, Thomas J, Suliman, Sara, Sutherland, Jayne S, Winter, Jill, Kuivaniemi, Helena, Walzl, Gerhard, Tromp, Gerard, Consortium, GC6-74, and Consortium, Catalysis TB Biomarkers

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Lung, Infectious Diseases, Rare Diseases, Clinical Research, Tuberculosis, Genetics, Detection, screening and diagnosis, Aetiology, 4.1 Discovery and preclinical testing of markers and technologies, 2.1 Biological and endogenous factors, Infection, Good Health and Well Being, Humans, Neutrophils, Positron Emission Tomography Computed Tomography, Neutrophil Activation, Mycobacterium tuberculosis, Tuberculosis, Lymph Node, GC6-74 Consortium, Catalysis TB Biomarkers Consortium, General Science & Technology
Abstract

Mycobacterium tuberculosis (M.tb) causes tuberculosis (TB) and remains one of the leading causes of mortality due to an infectious pathogen. Host immune responses have been implicated in driving the progression from infection to severe lung disease. We analyzed longitudinal RNA sequencing (RNAseq) data from the whole blood of 74 TB progressors whose samples were grouped into four six-month intervals preceding diagnosis (the GC6-74 study). We additionally analyzed RNAseq data from an independent cohort of 90 TB patients with positron emission tomography-computed tomography (PET-CT) scan results which were used to categorize them into groups with high and low levels of lung damage (the Catalysis TB Biomarker study). These groups were compared to non-TB controls to obtain a complete whole blood transcriptional profile for individuals spanning from early stages of M.tb infection to TB diagnosis. The results revealed a steady increase in the number of genes that were differentially expressed in progressors at time points closer to diagnosis with 278 genes at 13-18 months, 742 at 7-12 months and 5,131 detected 1-6 months before diagnosis and 9,205 detected in TB patients. A total of 2,144 differentially expressed genes were detected when comparing TB patients with high and low levels of lung damage. There was a large overlap in the genes upregulated in progressors 1-6 months before diagnosis (86%) with those in TB patients. A comprehensive pathway analysis revealed a potent activation of neutrophil and platelet mediated defenses including neutrophil and platelet degranulation, and NET formation at both time points. These pathways were also enriched in TB patients with high levels of lung damage compared to those with low. These findings suggest that neutrophils and platelets play a critical role in TB pathogenesis, and provide details of the timing of specific effector mechanisms that may contribute to TB lung pathology.

Published
2022

7. RISK6, a 6-gene transcriptomic signature of TB disease risk, diagnosis and treatment response

Author

Penn-Nicholson, Adam, Mbandi, Stanley Kimbung, Thompson, Ethan, Mendelsohn, Simon C, Suliman, Sara, Chegou, Novel N, Malherbe, Stephanus T, Darboe, Fatoumatta, Erasmus, Mzwandile, Hanekom, Willem A, Bilek, Nicole, Fisher, Michelle, Kaufmann, Stefan HE, Winter, Jill, Murphy, Melissa, Wood, Robin, Morrow, Carl, Van Rhijn, Ildiko, Moody, Branch, Murray, Megan, Andrade, Bruno B, Sterling, Timothy R, Sutherland, Jayne, Naidoo, Kogieleum, Padayatchi, Nesri, Walzl, Gerhard, Hatherill, Mark, Zak, Daniel, and Scriba, Thomas J

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Clinical Research, Prevention, Infectious Diseases, HIV/AIDS, Lung, Tuberculosis, Rare Diseases, 4.1 Discovery and preclinical testing of markers and technologies, Detection, screening and diagnosis, 4.2 Evaluation of markers and technologies, Infection, Good Health and Well Being, Adolescent, Area Under Curve, Biomarkers, Cohort Studies, Female, HIV Infections, Humans, Male, Mycobacterium tuberculosis, Point-of-Care Systems, Positron-Emission Tomography, Prognosis, RNA, Bacterial, ROC Curve, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Adolescent Cohort Study team, GC6-74 Consortium, SATVI Clinical and Laboratory Team, ScreenTB Consortium, AE-TBC Consortium, RePORT Brazil Team, Peruvian Household Contacts Cohort Team, CAPRISA IMPRESS team
Abstract

Improved tuberculosis diagnostics and tools for monitoring treatment response are urgently needed. We developed a robust and simple, PCR-based host-blood transcriptomic signature, RISK6, for multiple applications: identifying individuals at risk of incident disease, as a screening test for subclinical or clinical tuberculosis, and for monitoring tuberculosis treatment. RISK6 utility was validated by blind prediction using quantitative real-time (qRT) PCR in seven independent cohorts. Prognostic performance significantly exceeded that of previous signatures discovered in the same cohort. Performance for diagnosing subclinical and clinical disease in HIV-uninfected and HIV-infected persons, assessed by area under the receiver-operating characteristic curve, exceeded 85%. As a screening test for tuberculosis, the sensitivity at 90% specificity met or approached the benchmarks set out in World Health Organization target product profiles for non-sputum-based tests. RISK6 scores correlated with lung immunopathology activity, measured by positron emission tomography, and tracked treatment response, demonstrating utility as treatment response biomarker, while predicting treatment failure prior to treatment initiation. Performance of the test in capillary blood samples collected by finger-prick was noninferior to venous blood collected in PAXgene tubes. These results support incorporation of RISK6 into rapid, capillary blood-based point-of-care PCR devices for prospective assessment in field studies.

Published
2020

8. Cascade Immune Mechanisms of Protection against Mycobacterium tuberculosis (IMPAc-TB): study protocol for the Household Contact Study in the Western Cape, South Africa

Author

Hiemstra, Andriёtte M., MacDonald, Candice E., van Rensburg, Ilana C., Stanley, Kim, Maasdorp, Elizna, Mc Anda, Shirley, Tönsing, Susanne, Shaw, Jane Alexandra, Tromp, Gerard, van der Spuy, Gian D., Urdahl, Kevin B., Lewinsohn, David M., Kuivaniemi, Helena, Du Plessis, Nelita, Malherbe, Stephanus T., and Walzl, Gerhard

Published
2022
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9. Concurrent evaluation of cytokines improves the accuracy of antibodies against Mycobacterium tuberculosis antigens in the diagnosis of active tuberculosis

Author

Walzl, Gerhard, Chegou, Novel N., Kriel, Magdalena, van der Spuy, Gian, Loxton, Andre G., Stanley, Kim, Malherbe, Stephanus T., Kriel, Belinda, Sutherland, Jayne S., Owolabi, Olumuyiwa, Sillah, Abdou, Mendy, Joseph, Gindeh, Awa, Donkor, Simon, Togun, Toyin, Ota, Martin, Crampin, Amelia C., Simukonda, Felanji, Amberbir, Alemayehu, Chilongo, Femia, Houben, Rein, Kassa, Desta, Gebrezgeabher, Atsbeha, Mesfin, Getnet, Belay, Yohannes, Gebremichael, Gebremedhin, Alemayehu, Yodit, van der Vyver, Marieta, Amutenya, Faustina N., Nelongo, Josefina N., Monye, Lidia, Sheehama, Jacob A., Iipinge, Scholastica, Mayanja-Kizza, Harriet, Namuganga, Anna Ritah, Muzanye, Grace, Nsereko, Mary, Peters, Pierre, Howe, Rawleigh, Mihret, Adane, Bekele, Yonas, Tessema, Bamlak, Yamuah, Lawrence, Ottenhoff, Tom H.M., Geluk, Annemieke, Franken, Kees, van der Ploeg-van Schip, Jolien J., Corstjens, Paul L.A.M., Tjon Kon Fat, Elisa M., de Dood, Claudia J., Rosenkrands, Ida, Aagaard, Claus, Kaufmann, Stefan H.E., Esterhuyse, Maria M., Cliff, Jacqueline M., Dockrell, Hazel M., Jacobs, Ruschca, Awoniyi, Dolapo O., Baumann, Ralf, McAnda, Shirley, Kaempfer, Susanne, and Singh, Mahavir

Published
2022
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10. A novel aerosol collection method shows the cough aeromicrobiome of people with tuberculosis is phylogenetically distinct from respiratory tract specimens

Author

Chiyaka, Tinaye L., primary, Nyawo, Georgina R., additional, Naidoo, Charissa, additional, Moodley, Suventha, additional, Clemente, Jose C., additional, Malherbe, Stephanus T., additional, Warren, Robin, additional, Ku, David, additional, Segal, Leopoldo N., additional, and Theron, Grant, additional

Published
2024
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11. Radiological and functional evidence of the bronchial spread of tuberculosis: an observational analysis

Author

Chen, Ray Y, Yu, Xiang, Smith, Bronwyn, Liu, Xin, Gao, Jingcai, Diacon, Andreas H, Dawson, Rodney, Tameris, Michele, Zhu, Hong, Qu, Yahong, Zhang, Ruanqing, Pan, Shouguo, Jin, Xiaowei, Goldfeder, Lisa C, Cai, Ying, Arora, Kriti, Wang, Jing, Vincent, Joel, Malherbe, Stephanus T, Thienemann, Friedrich, Wilkinson, Robert J, Walzl, Gerhard, and Barry, Clifton E, III

Published
2021
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13. Validation of a host blood transcriptomic biomarker for pulmonary tuberculosis in people living with HIV: a prospective diagnostic and prognostic accuracy study

Author

Abrahams, Charmaine, Africa, Hadn, Ahlers, Petri, Arendsen, Denis, Badimo, Tebogo, Baepanye, Kagiso, Baepanye, Kesenogile Edna, Bande, Bianca, Batyi, Nomfuneko Cynthia, Beukes, Roslyn, Bontsi, Laudicia Tshenolo, Booi, Obakeng Peter, Botha, Mari Cathrin, Braaf, Samentra, Buhlungu, Sivuyile, Carstens, Alida, Chauke, Kgomotso Violet, Chinappa, Thilagavathy, Chung, Eva, Chung, Michelle, Clarke, Ken, Cloete, Yolundi, Coetzee, Lorraine, Collignon, Marelize, Companie, Alessandro, Corris, Cara-mia, Cwaile, Mooketsi Theophillius, Cwele, Thobelani, Davids, Ilse, Davies, Isabella Johanna, De Klerk, Emilia, de Kock, Marwou, Dhlamini, Audrey Lebohang, Diamond, Bongani, Didloff, Maria, Dlamini, Celaphiwe, Dolo, Palesa, Eyre, Candice, Feni, Tebogo, Ferreira, Juanita, Ferus, Christal, Fisher, Michelle, Flinn, Marika, Fransman, Bernadine, Galane, Welseh Phindile, Geldenhuys, Hennie, Gempies, Diann, Goliath, Thelma, Govender, Dhineshree, Gregg, Yolande, Gumede, Goodness, Gwamada, Zanele, Halti, Senzo, Hassiem, Rieyaat, Herling, Roxane, Herselman, Yulandi, Hughes, Ellis, Issel, Henry, Iyemosolo, Blanchard Mbay, Jali, Zandile, Janse Van Rensburg, Bonita, Jansen, Ruwiyda, Jeleni, James Michael, Jonkane, Olebogeng, Julies, Fabio, Kafaar, Fazlin, Kasongo, Christian Mabika, Keffers, Sophie, Kekana, Boitumelo Sophy, Kekana, Sebaetseng Jeanette, Kelepu, Xoliswa, Khanyile, Lungile, Khobedi, Gomotsegang Virginia, Khomba, Gloria, Khoza, Lucky Sipho, King, Marietjie, Kolobe, Gloria Keitumetse, Kruger, Sandra, Kruger, Jaftha, Kunene, Ndlela Israel, Lakay, Sunelza, Lakhi, Aneesa, Langa, Nondumiso, Ledwaba, Hildah, Lekagane, Lerato Julia, Lekotloane, Sheiley Christina, Leopeng, Thelma, Louw, Ilze Jeanette, Luabeya, Angelique Kany Kany, Lusale, Sarah Teboso, Maatjie, Perfect Tiisetso, Mabasa, Immaculate, Mabe, Tshegofatso Dorah, Mabena, Kamogelo Fortunate, Mabuza, Nkosinathi Charles, Mabwe, Simbarashe, Madikwe, Johanna Thapelo, Madikwe, Octavia Mahkosazana, Maebana, Rapontwana Letlhogonolo, Magwasha, Malobisa Sylvester, Majola, Molly, Makhetha, Mantai, Makhethe, Lebohang, Malay, Vernon, Manzini, Vutlhari-I-Vunhenha Fairlord, Maphanga, Jabu, Maphanga, Nonhle, Market, Juanita, Maroele, Isholedi Samuel, Masibi, Omphile Petunia, Mathabanzini, July Rocky, Mathode, Tendamudzimu Ivan, Matsane, Ellen Ditaba, Mbata, Lungile, Meyer, Faheema, Mhandire, Nyasha Karen, Miga, Thembisiwe, Mkhize, Nosisa Charity Thandeka, Mkhokho, Caroline, Mkwalase, Neo Hilda, Mnqonywa, Nondzakazi, Moche, Karabo, Modisaotsile, Brenda Matshidiso, Mokgetsengoane, Patricia Pakiso, Mokone, Selemeng Matseliso Carol, Molatlhegi, Kegomoditswe Magdeline, Molefe, Thuso Andrew, Moloko, Joseph Panie, Molosi, Kabelo, Molotsi, Motlatsi Evelyn, Montwedi, Tebogo Edwin, Monyemangene, Boikanyo Dinah, Mooketsi, Hellen Mokopi, Moses, Miriam, Mosito, Boitumelo, Mosito, Tshplpfelo Mapula, Mosweu, Ireen Lesebang, Mothaga, Primrose, Motlagomang, Banyana Olga, Mouton, Angelique, Mpofu, Onesisa, Mthembu, Funeka Nomvula, Mtlali, Mpho, Ndlovu, Nhlamulo, Ngcobo, Nompumelelo, Noble, Julia, Ntamo, Bantubonke Bertrum, Ntanjana, Gloria, Ntshauba, Tedrius, Opperman, Fajwa, Padayatchi, Nesri, Papalagae, Thandiwe, Petersen, Christel, Phakathi, Themba, Phatshwane, Mapule Ozma, Plaatjie, Patiswa, Pretorius, Abe, Rameetse, Victor Kgothatso, Ramjit, Dirhona, Ratangee, Frances, Ratangee, Maigan, Sanyaka, Pearl Nomsa, Sato, Alicia, Schoeman, Elisma, Schreuder, Constance, Seabela, Letlhogonolo, Segaetsho, Kelebogile Magdeline, Sein, Ni Ni, Selepe, Raesibe Agnes Pearl, Senne, Melissa Neo, September, Alison, September, Cashwin, Serake, Moeti, Shenje, Justin, Shezi, Thandiwe, Shezi, Sifiso Cornelius, Sing, Phindile, Singh, Chandrapharbha, Sithetho, Zona, Solomons, Dorothy, Stanley, Kim, Steyn, Marcia, Stofile, Bongiwe, Stryers, Sonia, Swanepoel, Liticia, Swarts, Anne, Thaba, Mando Mmakhora, Theko, Lethabo Collen, Thembela, Philile, Thompo, Mugwena, Toefy, Asma, Toto, Khayalethu, Tsagae, Dimakatso Sylvia, Tsamane, Ayanda, Tshikovhi, Vincent, Tswaile, Lebogang Isaac, Tyambetyu, Petrus, Tönsing, Susanne, Valley, Habibullah, van der Merwe, Linda, van Rooyen, Elma, Veldsman, Ashley, Veldtsman, Helen, Vollenhoven, Kelvin, Zaca, Londiwe, Zimri, Elaine, Zulu, Mbali, Mendelsohn, Simon C, Fiore-Gartland, Andrew, Penn-Nicholson, Adam, Mulenga, Humphrey, Mbandi, Stanley Kimbung, Borate, Bhavesh, Hadley, Katie, Hikuam, Chris, Musvosvi, Munyaradzi, Bilek, Nicole, Erasmus, Mzwandile, Jaxa, Lungisa, Raphela, Rodney, Nombida, Onke, Kaskar, Masooda, Sumner, Tom, White, Richard G, Innes, Craig, Brumskine, William, Hiemstra, Andriëtte, Malherbe, Stephanus T, Hassan-Moosa, Razia, Tameris, Michèle, Walzl, Gerhard, Naidoo, Kogieleum, Churchyard, Gavin, Scriba, Thomas J, and Hatherill, Mark

Published
2021
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14. Biomarker-guided tuberculosis preventive therapy (CORTIS): a randomised controlled trial

Author

Baepanye, Kesenogile, Baepanye, Tshepiso, Clarke, Ken, Collignon, Marelize, Dlamini, Audrey, Eyre, Candice, Feni, Tebogo, Fikizolo, Moogo, Galane, Phinda, Goliath, Thelma, Gangat, Alia, Malefo-Grootboom, Shirley, Janse van Rensburg, Elba, Janse van Rensburg, Bonita, Kekana, Sophy, Zietsman, Marietjie, Kock, Adrianne, Kunene, Israel, Lakhi, Aneessa, Langa, Nondumiso, Ledwaba, Hilda, Luphoko, Marillyn, Mabasa, Immaculate, Mabe, Dorah, Mabuza, Nkosinathi, Majola, Molly, Makhetha, Mantai, Makoanyane, Mpho, Makhubalo, Blossom, Malay, Vernon, Market, Juanita, Matshego, Selvy, Mbipa, Nontsikelelo, Mmotsa, Tsiamo, Modipa, Sylvester, Mopati, Samuel, Moswegu, Palesa, Mothaga, Primrose, Muller, Dorothy, Nchwe, Grace, Nel, Maryna, Nhlangulela, Lindiwe, Ntamo, Bantubonke, Ntoahae, Lawerence, Ntshauba, Tedrius, Sanyaka, Nomsa, Seabela, Letlhogonolo, Selepe, Pearl, Senne, Melissa, Serake, MG, Thlapi, Maria, Tshikovhi, Vincent, Tswaile, Lebogang, van Aswegen, Amanda, Mbata, Lungile, Takavamanya, Constance, Pinho, Pedro, Mdlulu, John, Taljaard, Marthinette, Slabbert, Naydene, Sayed, Sharfuddin, Nielson, Tanya, Ni Sein, Ni, Govender, Dhineshree, Chinappa, Tilagavathy, Zulu, Mbali Ignatia, Maphanga, Nonhle Bridgette, Hlathi, Senzo Ralph, Gumede, Goodness Khanyisile, Shezi, Thandiwe Yvonne, Maphanga, Jabulisiwe Lethabo, Jali, Zandile Patrica, Cwele, Thobelani, Gwamanda, Nonhlanhla Zanele Elsie, Dlamini, Celaphiwe, Sing, Zibuyile Phindile Penlee, Ntanjana, Ntombozuko Gloria, Nzimande, Sphelele Simo, Mbatha, Siyabonga, Maharaj, Bhavna, Moosa, Atika, Corris, Cara-Mia, Kafaar, Fazlin, Geldenhuys, Hennie, Luabeya, Angelique Kany Kany, Shenje, Justin, Botes, Natasja, Rossouw, Susan, Africa, Hadn, Diamond, Bongani, Braaf, Samentra, Stryers, Sonia, Carstens, Alida, Jansen, Ruwiyda, Mabwe, Simbarashe, Mulenga, Humphrey, Herling, Roxane, Veldsman, Ashley, Makhete, Lebohgang, Steyn, Marcia, Buhlungu, Sivuyile, Erasmus, Margareth, Davids, Ilse, Plaatjie, Patiswa, Companie, Alessandro, Ratangee, Frances, Veldtsman, Helen, Petersen, Christel, Abrahams, Charmaine, Moses, Miriam, Kelepu, Xoliswa, Gregg, Yolande, Swanepoel, Liticia, Magawu, Nomsitho, Cetywayo, Nompumelelo, Mactavie, Lauren, Valley, Habibullah, Filander, Elizabeth, Nqakala, Nambitha, Maasdorp, Elizna, Khoury, Justine, Kriel, Belinda, Smith, Bronwyn, Muller, Liesel, Tonsing, Susanne, Loxton, Andre, Hiemstra, Andriette, Ahlers, Petri, Flinn, Marika, Chung, Eva, Chung, Michelle, Sato, Alicia, Scriba, Thomas J, Fiore-Gartland, Andrew, Penn-Nicholson, Adam, Kimbung Mbandi, Stanley, Borate, Bhavesh, Mendelsohn, Simon C, Hadley, Katie, Hikuam, Chris, Kaskar, Masooda, Musvosvi, Munyaradzi, Bilek, Nicole, Self, Steven, Sumner, Tom, White, Richard G, Erasmus, Mzwandile, Jaxa, Lungisa, Raphela, Rodney, Innes, Craig, Brumskine, William, Hiemstra, Andriëtte, Malherbe, Stephanus T, Hassan-Moosa, Razia, Tameris, Michèle, Walzl, Gerhard, Naidoo, Kogieleum, Churchyard, Gavin, and Hatherill, Mark

Published
2021
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16. Expression and production of the SERPING1-encoded endogenous complement regulator C1-inhibitor in multiple cohorts of tuberculosis patients

Author

Lubbers, Rosalie, Sutherland, Jayne S., Goletti, Delia, de Paus, Roelof A., Dijkstra, Douwe J., van Moorsel, Coline H.M., Veltkamp, Marcel, Vestjens, Stefan M.T., Bos, Willem J.W., Petrone, Linda, Malherbe, Stephanus T., Walzl, Gerhard, Gelderman, Kyra A., Groeneveld, Geert H., Geluk, Annemieke, Ottenhoff, Tom H.M., Joosten, Simone A., and Trouw, Leendert A.

Published
2020
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17. Mycobacterium tuberculosis resisters despite HIV exhibit activated T cells and macrophages in their pulmonary alveoli

Author

Schurr, Erwin, primary, Dallmann-Sauer, Monica, additional, Fava, Vinicius, additional, Malherbe, Stephanus, additional, McDonald, Candice, additional, Orlova, Marianna, additional, Kroon, Elouise, additional, Cobat, Aurélie, additional, Boisson-Dupuis, Stéphanie, additional, Hoal, Eileen, additional, Abel, Laurent, additional, Möller, Marlo, additional, Casanova, Jean-Laurent, additional, Walzl, Gerhard, additional, and du Plessis, Nelita, additional

Published
2024
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18. Diabetes screen during tuberculosis contact investigations highlights opportunity for new diabetes diagnosis and reveals metabolic differences between ethnic groups

Author

Restrepo, Blanca I., Kleynhans, Léanie, Salinas, Alejandra B., Abdelbary, Bassent, Tshivhula, Happy, Aguillón-Durán, Genesis P., Kunsevi-Kilola, Carine, Salinas, Gloria, Stanley, Kim, Malherbe, Stephanus T., Maasdorp, Elizna, Garcia-Viveros, Moncerrato, Louw, Ilze, Garcia-Oropesa, Esperanza M., Lopez-Alvarenga, Juan Carlos, Prins, John B., Walzl, Gerhard, Schlesinger, Larry S., and Ronacher, Katharina

Published
2018
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20. Host blood RNA signatures predict the outcome of tuberculosis treatment

Author

Du Plessis, Nelita, Loxton, Andre G., Chegou, Novel N., Lee, Myungsun, Thompson, Ethan G., Du, Ying, Malherbe, Stephanus T., Shankar, Smitha, Braun, Jackie, Valvo, Joe, Ronacher, Katharina, Tromp, Gerard, Tabb, David L., Alland, David, Shenai, Shubhada, Via, Laura E., Warwick, James, Aderem, Alan, Scriba, Thomas J., Winter, Jill, Walzl, Gerhard, and Zak, Daniel E.

Published
2017
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22. High resolution imaging and five-year tuberculosis contact outcomes

Author

Esmail, Hanif, primary, Coussens, Anna K, additional, Thienemann, Friedrich, additional, Sossen, Bianca, additional, Mukasa, Sandra L, additional, Warwick, James, additional, Goliath, Rene T., additional, Davies, Nashreen Omar, additional, Douglass, Emily, additional, Jackson, Amanda, additional, Lakay, Francisco, additional, Streicher, Elizabeth, additional, Munro, Jacob E., additional, Barrios, Marilou H, additional, Heinsohn, Torben, additional, Macpherson, Liana, additional, Sheerin, Dylan, additional, Aziz, Saalikha, additional, Serole, Keboile, additional, Daroowala, Remy, additional, Taliep, Arshad, additional, Ahlers, Petri, additional, Malherbe, Stephanus T., additional, Bowden, Rory, additional, Warren, Robin, additional, Walzl, Gerhard, additional, Via, Laura E., additional, Bahlo, Melanie, additional, Jacobson, Karen R., additional, Horsburgh, C. Robert, additional, Salgame, Padmini, additional, Alland, David, additional, Barry, Clifton Earl, additional, Flynn, JoAnne L., additional, Ellner, Jerrold J, additional, and Wilkinson, Robert J, additional

Published
2023
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23. Immunologic and vascular biomarkers of mortality in critical COVID-19 in a South African cohort

Author

Shaw, Jane Alexandra, primary, Meiring, Maynard, additional, Snyders, Candice, additional, Everson, Frans, additional, Sigwadhi, Lovemore Nyasha, additional, Ngah, Veranyay, additional, Tromp, Gerard, additional, Allwood, Brian, additional, Koegelenberg, Coenraad F. N., additional, Irusen, Elvis M., additional, Lalla, Usha, additional, Baines, Nicola, additional, Zemlin, Annalise E., additional, Erasmus, Rajiv T., additional, Chapanduka, Zivanai C., additional, Matsha, Tandi E., additional, Walzl, Gerhard, additional, Strijdom, Hans, additional, du Plessis, Nelita, additional, Zumla, Alimuddin, additional, Chegou, Novel, additional, Malherbe, Stephanus T., additional, and Nyasulu, Peter S., additional

Published
2023
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24. Quantitative 18F-FDG PET-CT scan characteristics correlate with tuberculosis treatment response

Author

Malherbe, Stephanus T., Chen, Ray Y., Dupont, Patrick, Kant, Ilse, Kriel, Magdalena, Loxton, André G., Smith, Bronwyn, Beltran, Caroline G. G., van Zyl, Susan, McAnda, Shirely, Abrahams, Charmaine, Maasdorp, Elizna, Doruyter, Alex, Via, Laura E., Barry, III, Clifton E., Alland, David, Richards, Stephanie Griffith-, Ellman, Annare, Peppard, Thomas, Belisle, John, Tromp, Gerard, Ronacher, Katharina, Warwick, James M., Winter, Jill, and Walzl, Gerhard

Published
2020
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25. Accuracy of diabetes screening methods used for people with tuberculosis, Indonesia, Peru, Romania, South Africa/Precision des methodes de depistage du diabete utilisees pour les personnes atteintes de tuberculose en Afrique du Sud, en Indonesie, au Perou et en Roumanie/Precision de los metodos de diagnostico de diabetes utilizados en personas con tuberculosis en Indonesia, Peru, Rumania y Sudafrica

Author

Grint, Daniel, Alisjhabana, Bachti, Ugarte-Gil, Cesar, Riza, Anca-Leila, Walzl, Gerhard, Pearson, Fiona, Ruslami, Rovina, Moore, David A.J., Ioana, Mihai, McAllister, Susan, Ronacher, Katharina, Koeseomadinata, Raspati C., Kerry-Barnard, Sarah R., Coronel, Jorge, Malherbe, Stephanus T., Dockrell, Hazel M., Hill, Philip C., Van Crevel, Reinout, and Critchley, Julia A.

Subjects
Diabetes mellitus -- Diagnosis, Tuberculosis patients -- Health aspects -- Statistics, Hemoglobins, Health screening, Tuberculosis, Blood glucose, Pulmonary tuberculosis, Glycosylated hemoglobin, Medical tests, Exercise tests, Medical research, Glucose, Health, World Health Organization -- Services
Abstract

Objective To evaluate the performance of diagnostic tools for diabetes mellitus, Including laboratory methods and clinical risk scores, In newly-diagnosed pulmonary tuberculosis patients from four middle-income countries.Methods In a multicentre, prospective study, we recruited 2185 patients with pulmonary tuberculosis from sites in Indonesia, Peru, Romania ' and South Africa from January 2014 to September 2016. Using laboratory-measured glycated haemoglobin (HbA1c) as the gold standard, we measured the diagnostic accuracy of random plasma glucose, point-of-care HbA1c, fasting blood glucose, urine dipstick, published and newly derived diabetes mellitus risk scores and anthropometric measurements. We also analysed combinations of tests, including a two-step test using point-of-care HbA1cwhen Initial random plasma glucose was [greater than or equal to] 6.1 mmol/L.Findings The overall crude prevalence of diabetes mellitus among newly diagnosed tuberculosis patients was 283/2185 (13.0%; 95% confidence interval, CI: 11.6-14.4). The marker with the best diagnostic accuracy was point-of-care HbA1c (area under receiver operating characteristic curve: 0.81; 95% CI: 0.75-0.86). A risk score derived using age, point-of-care HbA1c and random plasma glucose had the best overall diagnostic accuracy (area under curve: 0.85; 95% CI: 0.81-0.90). There was substantial heterogeneity between sites for all markers, but the two-step combination test performed well in Indonesia and Peru.Conclusion Random plasma glucose followed by point-of-care HbA1c testing can accurately diagnose diabetes in tuberculosis patients, particularly those with substantial hyperglycaemla, while reducing the need for more expensive point-of-care HbA1c testing. Risk scores with or without biochemical data may be useful but require validation.Objectif Evaluer la performance des outils de diagnostic du diabete sucre, et notamment les methodes de laboratoire et les cotes de risque clinique, chez les patients souffrant d'une tuberculose pulmonaire recemment diagnostiquee dans quatre pays a revenu intermediaire.Methodes Dans le cadre d'une etude multicentrique et prospective, nous avons recrute 2185 patients atteints de tuberculose pulmonaire dans differents endroits en Afrique du Sud, en Indonesie, au Perou et en Roumanie entre janvier 2014 et septembre 2016. En utilisant comme referencel'hemoglobineglyquee (HbA1c) mesuree en laboratoire, nous avons evalue la precision diagnostique de la glycemie aleatoire, de l'HbAI c sur le lieu d'intervention, de la glycemie a jeun, de la bandelette urinaire, des cotes de risque deja publiees et recemment obtenues pour le diabete sucre et des mesures anthropometriques. Nous avons egalement analyse des combinaisons de tests, et notamment un test en deux etapes qui utilise l'HbA1c sur le lieu d'intervention lorsque la glycemie aleatoire initiale est [greater than or equal to] 6,1 mmol/LResultats La prevalence brute generale du diabete sucre chez les patients souffrant d'une tuberculose recemment diagnostiquee etait de 283/2185 (13,0%; intervalle de confiance, IC, a 95%: 11,6-14,4). Le marqueur fournissant la meilleure precision diagnostique etait l'HbA1c sur le lieu d'intervention (aire sous la courbe ROC: 0,81; IC a 95%: 0,75-0,86). La meilleure precision diagnostique generale a ete obtenue a l'aide d'une cote de risque calculee au moyen de l'age, de l'HbA1c sur le lieu d'intervention et de la glycemie aleatoire (aire sous la courbe ROC: 0,85; IC a 95%: 0,81-0,90). Une forte heterogeneite entre les differents endrolts a ete constatee pourtous les marqueurs, mais le test en deux etapes a donne de bons resultats en Indonesie et au Perou.Conclusion L'utilisation de la glycemie aleatoire suivie de l'HbA1c sur le lieu d'intervention permet de diagnostiquer avec precision le diabete chez les patients atteints de tuberculose, en particulier lorsqu'ils souffrent d'une importante hyperglycemie, tout en diminuant le besoin de tests HbA1c sur le lieu d'intervention, plus couteux. Les cotes de risque associees ou non a des donnees biochimiques peuvent etre utiles, mais doivent etre validees.Objetivo Evaluar el rendimiento de las herramientas de diagnostico para la diabetes mellitus, incluidos los metodos de laboratorio y los indices de riesgo clinico, en pacientes recien diagnosticados con tuberculosis pulmonar de cuatro paises de ingresos medios.Metodos En un estudio prospectivo multicentrico, se seleccionaron 2185 pacientes con tuberculosis pulmonar de algunas regiones de Indonesia, Peru, Rumania y Sudafrica entre enero de 2014 y septiembre de 2016. Usando hemoglobina glicosilada medida en laboratorio (HbA1 c) como el patron de excelencia, se midio la precision diagnostica de glucosa plasmatica al azar, estando HbA1 c en el punto de atencion, glucemia en sangre en ayunas, tira reactiva de orina, indices de riesgo de diabetes mellitus publicados recientemente y medidas antropometricas. Tambien se analizaron combinaciones de pruebas, incluida una prueba de dos pasos con HbA1c en el punto de atencion cuando la glucosa plasmatica aleatoria inicial fue [greater than or equal to] 6,1 mmol/L.Resultados La prevalencia general cruda de diabetes mellitus entre los pacientes con tuberculosis recien diagnosticados fue de 283/2185 (13,0 %, intervalo de confianza del 95 %, IC: 11,6-14,4). El marcador con la mejor precision diagnostica fue la HbA1c en el punto de atencion (area bajo la curva caracteristica operativa del receptor: 0,81; IC del 95 %: 0,75-0,86). Un indice de riesgo derivado de la edad, la HbA1c en el punto de atencion y la glucosa plasmatica al azar obtuvieron la mejor precision diagnostica general (area bajo la curva: 0,85; IC del 95 %: 0,81 -0,90). Hubo una heterogeneidad sustancial entre las regiones para todos los marcadores, pero la prueba combinada de dos pasos funciono de manera correcta en Indonesia y Peru.Conclusion La glucosa plasmatica al azar seguida de la prueba de HbA1c en el punto de atencion puede diagnosticar con precision la diabetes en pacientes con tuberculosis, particularmente en aquellos con hiperglucemia sustancial, mientras se reduce la necesidad de pruebas mas costosas de HbA1 c en el punto de atencion. Los indices de riesgo con o sin datos bioquimicos pueden ser utiles, pero requieren validacion., [phrase omitted]IntroductionTuberculosis is a major global public health concern. In 2016, the World Health Organization (WHO) estimated that there were 10.4 million new tuberculosis patients worldwide and 1.8 million tuberculosis-related [...]

Published
2018
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28. Impaired resolution of blood transcriptomes through tuberculosis treatment with diabetes comorbidity.

Author

Eckold, Clare, van Doorn, Cassandra L. R., Ruslami, Rovina, Ronacher, Katharina, Riza, Anca‐Lelia, van Veen, Suzanne, Lee, Ji‐Sook, Kumar, Vinod, Kerry‐Barnard, Sarah, Malherbe, Stephanus T., Kleynhans, Léanie, Stanley, Kim, Joosten, Simone A., Critchley, Julia A, Hill, Philip C., van Crevel, Reinout, Wijmenga, Cisca, Haks, Mariëlle C., Ioana, Mihai, and Alisjahbana, Bachti

Subjects
PEOPLE with diabetes, TRANSCRIPTOMES, TUBERCULOSIS, GENE expression, COMORBIDITY
Abstract

Background: People with diabetes are more likely to develop tuberculosis (TB) and to have poor TB‐treatment outcomes than those without. We previously showed that blood transcriptomes in people with TB‐diabetes (TB‐DM) co‐morbidity have excessive inflammatory and reduced interferon responses at diagnosis. It is unknown whether this persists through treatment and contributes to the adverse outcomes. Methods: Pulmonary TB patients recruited in South Africa, Indonesia and Romania were classified as having TB‐DM, TB with prediabetes, TB‐related hyperglycaemia or TB‐only, based on glycated haemoglobin concentration at TB diagnosis and after 6 months of TB treatment. Gene expression in blood at diagnosis and intervals throughout treatment was measured by unbiased RNA‐Seq and targeted Multiplex Ligation‐dependent Probe Amplification. Transcriptomic data were analysed by longitudinal mixed‐model regression to identify whether genes were differentially expressed between clinical groups through time. Predictive models of TB‐treatment response across groups were developed and cross‐tested. Results: Gene expression differed between TB and TB‐DM patients at diagnosis and was modulated by TB treatment in all clinical groups but to different extents, such that differences remained in TB‐DM relative to TB‐only throughout. Expression of some genes increased through TB treatment, whereas others decreased: some were persistently more highly expressed in TB‐DM and others in TB‐only patients. Genes involved in innate immune responses, anti‐microbial immunity and inflammation were significantly upregulated in people with TB‐DM throughout treatment. The overall pattern of change was similar across clinical groups irrespective of diabetes status, permitting models predictive of TB treatment to be developed. Conclusions: Exacerbated transcriptome changes in TB‐DM take longer to resolve during TB treatment, meaning they remain different from those in uncomplicated TB after treatment completion. This may indicate a prolonged inflammatory response in TB‐DM, requiring prolonged treatment or host‐directed therapy for complete cure. Development of transcriptome‐based biomarker signatures of TB‐treatment response should include people with diabetes for use across populations. [ABSTRACT FROM AUTHOR]

Published
2023
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29. Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research

Author

Shaw, Jane Alexandra, primary, Meiring, Maynard, additional, Allies, Devon, additional, Cruywagen, Lauren, additional, Fisher, Tarryn-Lee, additional, Kasavan, Kesheera, additional, Roos, Kelly, additional, Botha, Stefan Marc, additional, MacDonald, Candice, additional, Hiemstra, Andriёtte M., additional, Simon, Donald, additional, Rensburg, Ilana van, additional, Flinn, Marika, additional, Shabangu, Ayanda, additional, Kuivaniemi, Helena, additional, Tromp, Gerard, additional, Malherbe, Stephanus T., additional, Walzl, Gerhard, additional, du Plessis, Nelita, additional, and Consortium), Stellenbosch University Immunology Research Group Bronchoalv, additional

Published
2023
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30. Diagnostic Performance of Host Blood Transcriptomic Signatures for Pulmonary Tuberculosis in a Multi-Cohort Study of Symptomatic Patients in Africa

Author

Muwanga, Vanessa, primary, Mendelsohn, Simon C., additional, Leukes, Vinzeigh, additional, Stanley, Kim, additional, Mbandi, Stanley Kimbung, additional, Erasmus, Mzwandile, additional, Flinn, Marika, additional, Fisher, Tarryn-Lee, additional, Raphela, Rodney, additional, Bilek, Nicole, additional, Malherbe, Stephanus T., additional, Tromp, Gerard, additional, Van der Spuy, Gian, additional, Walzl, Gerhard, additional, Chegou, Novel N., additional, and Scriba, Thomas J., additional

Published
2023
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31. Understanding the biology, morbidity and social contexts of adolescent tuberculosis: a prospective observational cohort study protocol (Teen TB)

Author

Swanepoel, Jeremi, primary, Zimri, Klassina, additional, van der Zalm, Marieke M, additional, Hoddinott, Graeme, additional, Palmer, Megan, additional, Doruyter, Alex, additional, De Beer, Gezila, additional, Kleynhans, Leanie, additional, Johnson, Sarah M, additional, Jongen, Vita, additional, Wademan, Dillon, additional, Mcimeli, Khanyisa, additional, Jacobs, Stephanie, additional, Swanepoel, Ruan, additional, Van Zyl, Gert, additional, Allwood, Brian W, additional, Malherbe, Stephanus, additional, Heuvelings, Charlotte, additional, Griffith-Richards, Stephanie, additional, Whittaker, Elizabeth, additional, Moore, David A J, additional, Schaaf, H Simon, additional, Hesseling, Anneke C, additional, and Seddon, James A, additional

Published
2022
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32. cis-eQTL mapping of TB-T2D comorbidity elucidates the involvement of African ancestry in TB susceptibility

Author

Swart, Yolandi, primary, Uren, Caitlin, additional, Eckold, Clare, additional, Cliff, Jacqueline M, additional, Malherbe, Stephanus T, additional, Ronacher, Katharina, additional, Kumar, Vinod, additional, Wijmenga, Cisca, additional, Dockrell, Hazel M, additional, van Crevel, Reinout, additional, Walzl, Gerhard, additional, Kleynhans, Léanie, additional, and Möller, Marlo, additional

Published
2022
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33. A semi-automatic technique to quantify complex tuberculous lung lesions on 18F-fluorodeoxyglucose positron emission tomography/computerised tomography images

Author

Malherbe, Stephanus T., Dupont, Patrick, Kant, Ilse, Ahlers, Petri, Kriel, Magdalena, Loxton, André G., Chen, Ray Y., Via, Laura E., Thienemann, Friedrich, Wilkinson, Robert J., Barry, III, Clifton E., Griffith-Richards, Stephanie, Ellman, Annare, Ronacher, Katharina, Winter, Jill, Walzl, Gerhard, Warwick, James M., and the Catalysis Biomarker Consortium

Published
2018
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34. Persisting positron emission tomography lesion activity and Mycobacterium tuberculosis mRNA after tuberculosis cure

Author

Malherbe, Stephanus T, Shenai, Shubhada, Ronacher, Katharina, Loxton, Andre G, Dolganov, Gregory, Kriel, Magdalena, Van, Tran, Chen, Ray Y, Warwick, James, Via, Laura E, Song, Taeksun, Lee, Myungsun, Schoolnik, Gary, Tromp, Gerard, Alland, David, Barry, Clifton E, III, Winter, Jill, Walzl, Gerhard, Lucas, Lance, Spuy, Gian van der, Stanley, Kim, Theart, Lani, Smith, Bronwyn, Du Plessis, Nelita, Beltran, Caroline G G, Maasdorp, Elizna, Ellmann, Annare, Choi, Hongjo, Joh, Joonsung, Dodd, Lori E, Allwood, Brian, Kogelenberg, Coenie, Vorster, Morné, and Griffith-Richards, Stephanie

Subjects
Positron emission tomography -- Usage -- Analysis, Tuberculosis -- Research, Messenger RNA -- Research, Mycobacterium tuberculosis -- Care and treatment, Biological sciences, Health
Abstract

The absence of a gold standard to determine when antibiotics induce a sterilizing cure has confounded the development of new approaches to treat pulmonary tuberculosis (PTB). We detected positron emission tomography and computerized tomography (PET-CT) imaging response patterns consistent with active disease, along with the presence of Mycobacterium tuberculosis (MTB) mRNA in sputum and bronchoalveolar lavage samples, in a substantial proportion of adult, HIV-negative patients with PTB after a standard 6-month treatment plus 1 year follow-up, including patients with a durable cure and others who later developed recurrent disease. The presence of MTB mRNA in the context of nonresolving and intensifying lesions on PET-CT images might indicate ongoing transcription, suggesting that even apparently curative treatment for PTB may not eradicate all of the MTB bacteria in most patients. This suggests an important complementary role for the immune response in maintaining a disease-free state. Sterilizing drugs or host-directed therapies, and better treatment response markers, are probably needed for the successful development of improved and shortened PTB-treatment strategies., Author(s): Stephanus T Malherbe (corresponding author) [1, 2]; Shubhada Shenai [3]; Katharina Ronacher [1, 2]; Andre G Loxton [1, 2]; Gregory Dolganov [4]; Magdalena Kriel [1, 2]; Tran Van [4]; [...]

Published
2016
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35. Transcriptional profiles predict treatment outcome in patients with tuberculosis and diabetes at diagnosis and at two weeks after initiation of anti-tuberculosis treatment

Author

van Doorn, Cassandra L.R., primary, Eckold, Clare, additional, Ronacher, Katharina, additional, Ruslami, Rovina, additional, van Veen, Suzanne, additional, Lee, Ji-Sook, additional, Kumar, Vinod, additional, Kerry-Barnard, Sarah, additional, Malherbe, Stephanus T., additional, Kleynhans, Léanie, additional, Stanley, Kim, additional, Hill, Philip C., additional, Joosten, Simone A., additional, van Crevel, Reinout, additional, Wijmenga, Cisca, additional, Critchley, Julia A., additional, Walzl, Gerhard, additional, Alisjahbana, Bachti, additional, Haks, Mariëlle C., additional, Dockrell, Hazel M., additional, Ottenhoff, Tom H.M., additional, Vianello, Eleonora, additional, and Cliff, Jacqueline M., additional

Published
2022
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36. Sildenafil, a Type-5 Phosphodiesterase Inhibitor, Fails to Reverse Myeloid-Derived Suppressor Cell-Mediated T Cell Suppression in Cells Isolated From Tuberculosis Patients

Author

Leukes, Vinzeigh N., primary, Malherbe, Stephanus T., additional, Hiemstra, Andriette, additional, Kotze, Leigh A., additional, Roos, Kelly, additional, Keyser, Alana, additional, De Swardt, Dalene, additional, Gutschmidt, Andrea, additional, Walzl, Gerhard, additional, and du Plessis, Nelita, additional

Published
2022
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37. Targeted Gene Expression Profiling of Human Myeloid Cells From Blood and Lung Compartments of Patients With Tuberculosis and Other Lung Diseases

Author

Kotze, Leigh Ann, primary, van der Spuy, Gian, additional, Leonard, Bryan, additional, Penn-Nicholson, Adam, additional, Musvosvi, Munyaradzi, additional, McAnda, Shirley, additional, Malherbe, Stephanus T., additional, Erasmus, Mzwandile, additional, Scriba, Thomas, additional, Koegelenberg, Coenraad F. N., additional, Allwood, Brian W., additional, Walzl, Gerhard, additional, and du Plessis, Nelita, additional

Published
2022
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38. Concurrent evaluation of cytokines improves the accuracy of antibodies against Mycobacterium tuberculosis antigens in the diagnosis of active tuberculosis

Author

Jacobs, Ruschca, primary, Awoniyi, Dolapo O., additional, Baumann, Ralf, additional, Stanley, Kim, additional, McAnda, Shirley, additional, Kaempfer, Susanne, additional, Malherbe, Stephanus T., additional, Singh, Mahavir, additional, Walzl, Gerhard, additional, Chegou, Novel N., additional, Kriel, Magdalena, additional, van der Spuy, Gian, additional, Loxton, Andre G., additional, Kriel, Belinda, additional, Sutherland, Jayne S., additional, Owolabi, Olumuyiwa, additional, Sillah, Abdou, additional, Mendy, Joseph, additional, Gindeh, Awa, additional, Donkor, Simon, additional, Togun, Toyin, additional, Ota, Martin, additional, Crampin, Amelia C., additional, Simukonda, Felanji, additional, Amberbir, Alemayehu, additional, Chilongo, Femia, additional, Houben, Rein, additional, Kassa, Desta, additional, Gebrezgeabher, Atsbeha, additional, Mesfin, Getnet, additional, Belay, Yohannes, additional, Gebremichael, Gebremedhin, additional, Alemayehu, Yodit, additional, van der Vyver, Marieta, additional, Amutenya, Faustina N., additional, Nelongo, Josefina N., additional, Monye, Lidia, additional, Sheehama, Jacob A., additional, Iipinge, Scholastica, additional, Mayanja-Kizza, Harriet, additional, Namuganga, Anna Ritah, additional, Muzanye, Grace, additional, Nsereko, Mary, additional, Peters, Pierre, additional, Howe, Rawleigh, additional, Mihret, Adane, additional, Bekele, Yonas, additional, Tessema, Bamlak, additional, Yamuah, Lawrence, additional, Ottenhoff, Tom H.M., additional, Geluk, Annemieke, additional, Franken, Kees, additional, van der Ploeg-van Schip, Jolien J., additional, Corstjens, Paul L.A.M., additional, Tjon Kon Fat, Elisa M., additional, de Dood, Claudia J., additional, Rosenkrands, Ida, additional, Aagaard, Claus, additional, Kaufmann, Stefan H.E., additional, Esterhuyse, Maria M., additional, Cliff, Jacqueline M., additional, and Dockrell, Hazel M., additional

Published
2022
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39. Author Correction: A multi-cohort study of the immune factors associated with M. tuberculosis infection outcomes

Author

Roy Chowdhury, Roshni, Vallania, Francesco, Yang, Qianting, Lopez Angel, Cesar Joel, Darboe, Fatoumatta, Penn-Nicholson, Adam, Rozot, Virginie, Nemes, Elisa, Malherbe, Stephanus T., Ronacher, Katharina, Walzl, Gerhard, Hanekom, Willem, Davis, Mark M., Winter, Jill, Chen, Xinchun, Scriba, Thomas J., Khatri, Purvesh, and Chien, Yueh-hsiu

Published
2018
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40. Additional file 2 of Cascade Immune Mechanisms of Protection against Mycobacterium tuberculosis (IMPAc-TB): study protocol for the Household Contact Study in the Western Cape, South Africa

Author

Hiemstra, Andriёtte M., MacDonald, Candice E., van Rensburg, Ilana C., Stanley, Kim, Maasdorp, Elizna, Mc Anda, Shirley, Tönsing, Susanne, Shaw, Jane Alexandra, Tromp, Gerard, van der Spuy, Gian D., Urdahl, Kevin B., Lewinsohn, David M., Kuivaniemi, Helena, Du Plessis, Nelita, Malherbe, Stephanus T., and Walzl, Gerhard

Subjects
Data_FILES, Hardware_ARITHMETICANDLOGICSTRUCTURES
Abstract

Additional file 2. Cascade data management plan.

Published
2022
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41. Additional file 1 of Cascade Immune Mechanisms of Protection against Mycobacterium tuberculosis (IMPAc-TB): study protocol for the Household Contact Study in the Western Cape, South Africa

Author

Hiemstra, Andriёtte M., MacDonald, Candice E., van Rensburg, Ilana C., Stanley, Kim, Maasdorp, Elizna, Mc Anda, Shirley, Tönsing, Susanne, Shaw, Jane Alexandra, Tromp, Gerard, van der Spuy, Gian D., Urdahl, Kevin B., Lewinsohn, David M., Kuivaniemi, Helena, Du Plessis, Nelita, Malherbe, Stephanus T., and Walzl, Gerhard

Abstract

Additional file 1. Cascade bronchoscopy source document and discharge summary.

Published
2022
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42. Additional file 3 of Cascade Immune Mechanisms of Protection against Mycobacterium tuberculosis (IMPAc-TB): study protocol for the Household Contact Study in the Western Cape, South Africa

Author

Hiemstra, Andriёtte M., MacDonald, Candice E., van Rensburg, Ilana C., Stanley, Kim, Maasdorp, Elizna, Mc Anda, Shirley, Tönsing, Susanne, Shaw, Jane Alexandra, Tromp, Gerard, van der Spuy, Gian D., Urdahl, Kevin B., Lewinsohn, David M., Kuivaniemi, Helena, Du Plessis, Nelita, Malherbe, Stephanus T., and Walzl, Gerhard

Subjects
TheoryofComputation_MISCELLANEOUS, Data_FILES, Data_CODINGANDINFORMATIONTHEORY, Hardware_ARITHMETICANDLOGICSTRUCTURES
Abstract

Additional file 3. Cascade data dictionary.

Published
2022
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45. Establishment of a Patient-Derived, Magnetic Levitation-Based, Three-Dimensional Spheroid Granuloma Model for Human Tuberculosis

Author

Kotze, Leigh A., primary, Beltran, Caroline G. G., additional, Lang, Dirk, additional, Loxton, Andre G., additional, Cooper, Susan, additional, Meiring, Maynard, additional, Koegelenberg, Coenraad F. N., additional, Allwood, Brian W., additional, Malherbe, Stephanus T., additional, Hiemstra, Andriette M., additional, Glanzmann, Brigitte, additional, Kinnear, Craig, additional, Walzl, Gerhard, additional, and du Plessis, Nelita, additional

Published
2021
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47. Validation of a host blood transcriptomic biomarker for pulmonary tuberculosis in people living with HIV: a prospective diagnostic and prognostic accuracy study

Author

Mendelsohn, Simon C, primary, Fiore-Gartland, Andrew, additional, Penn-Nicholson, Adam, additional, Mulenga, Humphrey, additional, Mbandi, Stanley Kimbung, additional, Borate, Bhavesh, additional, Hadley, Katie, additional, Hikuam, Chris, additional, Musvosvi, Munyaradzi, additional, Bilek, Nicole, additional, Erasmus, Mzwandile, additional, Jaxa, Lungisa, additional, Raphela, Rodney, additional, Nombida, Onke, additional, Kaskar, Masooda, additional, Sumner, Tom, additional, White, Richard G, additional, Innes, Craig, additional, Brumskine, William, additional, Hiemstra, Andriëtte, additional, Malherbe, Stephanus T, additional, Hassan-Moosa, Razia, additional, Tameris, Michèle, additional, Walzl, Gerhard, additional, Naidoo, Kogieleum, additional, Churchyard, Gavin, additional, Scriba, Thomas J, additional, Hatherill, Mark, additional, Abrahams, Charmaine, additional, Africa, Hadn, additional, Ahlers, Petri, additional, Arendsen, Denis, additional, Badimo, Tebogo, additional, Baepanye, Kagiso, additional, Baepanye, Kesenogile Edna, additional, Bande, Bianca, additional, Batyi, Nomfuneko Cynthia, additional, Beukes, Roslyn, additional, Bontsi, Laudicia Tshenolo, additional, Booi, Obakeng Peter, additional, Botha, Mari Cathrin, additional, Braaf, Samentra, additional, Buhlungu, Sivuyile, additional, Carstens, Alida, additional, Chauke, Kgomotso Violet, additional, Chinappa, Thilagavathy, additional, Chung, Eva, additional, Chung, Michelle, additional, Clarke, Ken, additional, Cloete, Yolundi, additional, Coetzee, Lorraine, additional, Collignon, Marelize, additional, Companie, Alessandro, additional, Corris, Cara-mia, additional, Cwaile, Mooketsi Theophillius, additional, Cwele, Thobelani, additional, Davids, Ilse, additional, Davies, Isabella Johanna, additional, De Klerk, Emilia, additional, de Kock, Marwou, additional, Dhlamini, Audrey Lebohang, additional, Diamond, Bongani, additional, Didloff, Maria, additional, Dlamini, Celaphiwe, additional, Dolo, Palesa, additional, Eyre, Candice, additional, Feni, Tebogo, additional, Ferreira, Juanita, additional, Ferus, Christal, additional, Fisher, Michelle, additional, Flinn, Marika, additional, Fransman, Bernadine, additional, Galane, Welseh Phindile, additional, Geldenhuys, Hennie, additional, Gempies, Diann, additional, Goliath, Thelma, additional, Govender, Dhineshree, additional, Gregg, Yolande, additional, Gumede, Goodness, additional, Gwamada, Zanele, additional, Halti, Senzo, additional, Hassiem, Rieyaat, additional, Herling, Roxane, additional, Herselman, Yulandi, additional, Hughes, Ellis, additional, Issel, Henry, additional, Iyemosolo, Blanchard Mbay, additional, Jali, Zandile, additional, Janse Van Rensburg, Bonita, additional, Jansen, Ruwiyda, additional, Jeleni, James Michael, additional, Jonkane, Olebogeng, additional, Julies, Fabio, additional, Kafaar, Fazlin, additional, Kasongo, Christian Mabika, additional, Keffers, Sophie, additional, Kekana, Boitumelo Sophy, additional, Kekana, Sebaetseng Jeanette, additional, Kelepu, Xoliswa, additional, Khanyile, Lungile, additional, Khobedi, Gomotsegang Virginia, additional, Khomba, Gloria, additional, Khoza, Lucky Sipho, additional, King, Marietjie, additional, Kolobe, Gloria Keitumetse, additional, Kruger, Sandra, additional, Kruger, Jaftha, additional, Kunene, Ndlela Israel, additional, Lakay, Sunelza, additional, Lakhi, Aneesa, additional, Langa, Nondumiso, additional, Ledwaba, Hildah, additional, Lekagane, Lerato Julia, additional, Lekotloane, Sheiley Christina, additional, Leopeng, Thelma, additional, Louw, Ilze Jeanette, additional, Luabeya, Angelique Kany Kany, additional, Lusale, Sarah Teboso, additional, Maatjie, Perfect Tiisetso, additional, Mabasa, Immaculate, additional, Mabe, Tshegofatso Dorah, additional, Mabena, Kamogelo Fortunate, additional, Mabuza, Nkosinathi Charles, additional, Mabwe, Simbarashe, additional, Madikwe, Johanna Thapelo, additional, Madikwe, Octavia Mahkosazana, additional, Maebana, Rapontwana Letlhogonolo, additional, Magwasha, Malobisa Sylvester, additional, Majola, Molly, additional, Makhetha, Mantai, additional, Makhethe, Lebohang, additional, Malay, Vernon, additional, Manzini, Vutlhari-I-Vunhenha Fairlord, additional, Maphanga, Jabu, additional, Maphanga, Nonhle, additional, Market, Juanita, additional, Maroele, Isholedi Samuel, additional, Masibi, Omphile Petunia, additional, Mathabanzini, July Rocky, additional, Mathode, Tendamudzimu Ivan, additional, Matsane, Ellen Ditaba, additional, Mbata, Lungile, additional, Meyer, Faheema, additional, Mhandire, Nyasha Karen, additional, Miga, Thembisiwe, additional, Mkhize, Nosisa Charity Thandeka, additional, Mkhokho, Caroline, additional, Mkwalase, Neo Hilda, additional, Mnqonywa, Nondzakazi, additional, Moche, Karabo, additional, Modisaotsile, Brenda Matshidiso, additional, Mokgetsengoane, Patricia Pakiso, additional, Mokone, Selemeng Matseliso Carol, additional, Molatlhegi, Kegomoditswe Magdeline, additional, Molefe, Thuso Andrew, additional, Moloko, Joseph Panie, additional, Molosi, Kabelo, additional, Molotsi, Motlatsi Evelyn, additional, Montwedi, Tebogo Edwin, additional, Monyemangene, Boikanyo Dinah, additional, Mooketsi, Hellen Mokopi, additional, Moses, Miriam, additional, Mosito, Boitumelo, additional, Mosito, Tshplpfelo Mapula, additional, Mosweu, Ireen Lesebang, additional, Mothaga, Primrose, additional, Motlagomang, Banyana Olga, additional, Mouton, Angelique, additional, Mpofu, Onesisa, additional, Mthembu, Funeka Nomvula, additional, Mtlali, Mpho, additional, Ndlovu, Nhlamulo, additional, Ngcobo, Nompumelelo, additional, Noble, Julia, additional, Ntamo, Bantubonke Bertrum, additional, Ntanjana, Gloria, additional, Ntshauba, Tedrius, additional, Opperman, Fajwa, additional, Padayatchi, Nesri, additional, Papalagae, Thandiwe, additional, Petersen, Christel, additional, Phakathi, Themba, additional, Phatshwane, Mapule Ozma, additional, Plaatjie, Patiswa, additional, Pretorius, Abe, additional, Rameetse, Victor Kgothatso, additional, Ramjit, Dirhona, additional, Ratangee, Frances, additional, Ratangee, Maigan, additional, Sanyaka, Pearl Nomsa, additional, Sato, Alicia, additional, Schoeman, Elisma, additional, Schreuder, Constance, additional, Seabela, Letlhogonolo, additional, Segaetsho, Kelebogile Magdeline, additional, Sein, Ni Ni, additional, Selepe, Raesibe Agnes Pearl, additional, Senne, Melissa Neo, additional, September, Alison, additional, September, Cashwin, additional, Serake, Moeti, additional, Shenje, Justin, additional, Shezi, Thandiwe, additional, Shezi, Sifiso Cornelius, additional, Sing, Phindile, additional, Singh, Chandrapharbha, additional, Sithetho, Zona, additional, Solomons, Dorothy, additional, Stanley, Kim, additional, Steyn, Marcia, additional, Stofile, Bongiwe, additional, Stryers, Sonia, additional, Swanepoel, Liticia, additional, Swarts, Anne, additional, Thaba, Mando Mmakhora, additional, Theko, Lethabo Collen, additional, Thembela, Philile, additional, Thompo, Mugwena, additional, Toefy, Asma, additional, Toto, Khayalethu, additional, Tsagae, Dimakatso Sylvia, additional, Tsamane, Ayanda, additional, Tshikovhi, Vincent, additional, Tswaile, Lebogang Isaac, additional, Tyambetyu, Petrus, additional, Tönsing, Susanne, additional, Valley, Habibullah, additional, van der Merwe, Linda, additional, van Rooyen, Elma, additional, Veldsman, Ashley, additional, Veldtsman, Helen, additional, Vollenhoven, Kelvin, additional, Zaca, Londiwe, additional, Zimri, Elaine, additional, and Zulu, Mbali, additional

Published
2021
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49. Diagnostic Accuracy of the Cepheid 3-gene Host Response Fingerstick Blood Test in a Prospective, Multi-site Study: Interim Results.

Author

Sutherland, Jayne S, van der Spuy, Gian, Gindeh, Awa, Thuong, Nguyen Thuy Thuong, Namuganga, AnnRitah, Owolabi, Olumuyiwa, Mayanja-Kizza, Harriet, Nsereko, Mary, Thwaites, Guy, Winter, Jill, Dockrell, Hazel M, Scriba, Thomas J, Geluk, Annemieke, Corstjens, Paul, Stanley, Kim, Richardson, Tracy, Shaw, Jane A, Smith, Bronwyn, Malherbe, Stephanus T, and Walzl, Gerhard

Subjects
HIV infection complications, BLOOD sugar analysis, TUBERCULOSIS diagnosis, MOLECULAR diagnosis, CONFIDENCE intervals, POINT-of-care testing, GENE expression, MESSENGER RNA, DESCRIPTIVE statistics, SENSITIVITY & specificity (Statistics), RECEIVER operating characteristic curves
Abstract

Background The development of a fast and accurate, non-sputum-based point-of-care triage test for tuberculosis (TB) would have a major impact on combating the TB burden worldwide. A new fingerstick blood test has been developed by Cepheid (the Xpert MTB Host Response [MTB-HR] prototype), which generates a "TB score" based on messenger RNA (mRNA) expression of 3 genes. Here we describe the first prospective findings of the MTB-HR prototype. Methods Fingerstick blood from adults presenting with symptoms compatible with TB in South Africa, The Gambia, Uganda, and Vietnam was analyzed using the Cepheid GeneXpert MTB-HR prototype. Accuracy of the Xpert MTB-HR cartridge was determined in relation to GeneXpert Ultra results and a composite microbiological score (GeneXpert Ultra and liquid culture) with patients classified as having TB or other respiratory diseases (ORD). Results When data from all sites (n = 75 TB, 120 ORD) were analyzed, the TB score discriminated between TB and ORD with an area under the curve (AUC) of 0.94 (95% confidence interval [CI],.91–.97), sensitivity of 87% (95% CI, 77–93%) and specificity of 94% (88–97%). When sensitivity was set at 90% for a triage test, specificity was 86% (95% CI, 75–97%). These results were not influenced by human immunodeficiency virus (HIV) status or geographical location. When evaluated against a composite microbiological score (n = 80 TB, 111 ORD), the TB score was able to discriminate between TB and ORD with an AUC of 0.88 (95% CI,.83–.94), 80% sensitivity (95% CI, 76–85%) and 94% specificity (95% CI, 91–96%). Conclusions Our interim data indicate the Cepheid MTB-HR cartridge reaches the minimal target product profile for a point of care triage test for TB using fingerstick blood, regardless of geographic area or HIV infection status. [ABSTRACT FROM AUTHOR]

Published
2022
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50. Biomarker-guided tuberculosis preventive therapy (CORTIS): a randomised controlled trial

Author

Scriba, Thomas J, primary, Fiore-Gartland, Andrew, additional, Penn-Nicholson, Adam, additional, Mulenga, Humphrey, additional, Kimbung Mbandi, Stanley, additional, Borate, Bhavesh, additional, Mendelsohn, Simon C, additional, Hadley, Katie, additional, Hikuam, Chris, additional, Kaskar, Masooda, additional, Musvosvi, Munyaradzi, additional, Bilek, Nicole, additional, Self, Steven, additional, Sumner, Tom, additional, White, Richard G, additional, Erasmus, Mzwandile, additional, Jaxa, Lungisa, additional, Raphela, Rodney, additional, Innes, Craig, additional, Brumskine, William, additional, Hiemstra, Andriëtte, additional, Malherbe, Stephanus T, additional, Hassan-Moosa, Razia, additional, Tameris, Michèle, additional, Walzl, Gerhard, additional, Naidoo, Kogieleum, additional, Churchyard, Gavin, additional, Hatherill, Mark, additional, Baepanye, Kesenogile, additional, Baepanye, Tshepiso, additional, Clarke, Ken, additional, Collignon, Marelize, additional, Dlamini, Audrey, additional, Eyre, Candice, additional, Feni, Tebogo, additional, Fikizolo, Moogo, additional, Galane, Phinda, additional, Goliath, Thelma, additional, Gangat, Alia, additional, Malefo-Grootboom, Shirley, additional, Janse van Rensburg, Elba, additional, Janse van Rensburg, Bonita, additional, Kekana, Sophy, additional, Zietsman, Marietjie, additional, Kock, Adrianne, additional, Kunene, Israel, additional, Lakhi, Aneessa, additional, Langa, Nondumiso, additional, Ledwaba, Hilda, additional, Luphoko, Marillyn, additional, Mabasa, Immaculate, additional, Mabe, Dorah, additional, Mabuza, Nkosinathi, additional, Majola, Molly, additional, Makhetha, Mantai, additional, Makoanyane, Mpho, additional, Makhubalo, Blossom, additional, Malay, Vernon, additional, Market, Juanita, additional, Matshego, Selvy, additional, Mbipa, Nontsikelelo, additional, Mmotsa, Tsiamo, additional, Modipa, Sylvester, additional, Mopati, Samuel, additional, Moswegu, Palesa, additional, Mothaga, Primrose, additional, Muller, Dorothy, additional, Nchwe, Grace, additional, Nel, Maryna, additional, Nhlangulela, Lindiwe, additional, Ntamo, Bantubonke, additional, Ntoahae, Lawerence, additional, Ntshauba, Tedrius, additional, Sanyaka, Nomsa, additional, Seabela, Letlhogonolo, additional, Selepe, Pearl, additional, Senne, Melissa, additional, Serake, MG, additional, Thlapi, Maria, additional, Tshikovhi, Vincent, additional, Tswaile, Lebogang, additional, van Aswegen, Amanda, additional, Mbata, Lungile, additional, Takavamanya, Constance, additional, Pinho, Pedro, additional, Mdlulu, John, additional, Taljaard, Marthinette, additional, Slabbert, Naydene, additional, Sayed, Sharfuddin, additional, Nielson, Tanya, additional, Ni Sein, Ni, additional, Govender, Dhineshree, additional, Chinappa, Tilagavathy, additional, Zulu, Mbali Ignatia, additional, Maphanga, Nonhle Bridgette, additional, Hlathi, Senzo Ralph, additional, Gumede, Goodness Khanyisile, additional, Shezi, Thandiwe Yvonne, additional, Maphanga, Jabulisiwe Lethabo, additional, Jali, Zandile Patrica, additional, Cwele, Thobelani, additional, Gwamanda, Nonhlanhla Zanele Elsie, additional, Dlamini, Celaphiwe, additional, Sing, Zibuyile Phindile Penlee, additional, Ntanjana, Ntombozuko Gloria, additional, Nzimande, Sphelele Simo, additional, Mbatha, Siyabonga, additional, Maharaj, Bhavna, additional, Moosa, Atika, additional, Corris, Cara-Mia, additional, Kafaar, Fazlin, additional, Geldenhuys, Hennie, additional, Luabeya, Angelique Kany Kany, additional, Shenje, Justin, additional, Botes, Natasja, additional, Rossouw, Susan, additional, Africa, Hadn, additional, Diamond, Bongani, additional, Braaf, Samentra, additional, Stryers, Sonia, additional, Carstens, Alida, additional, Jansen, Ruwiyda, additional, Mabwe, Simbarashe, additional, Herling, Roxane, additional, Veldsman, Ashley, additional, Makhete, Lebohgang, additional, Steyn, Marcia, additional, Buhlungu, Sivuyile, additional, Erasmus, Margareth, additional, Davids, Ilse, additional, Plaatjie, Patiswa, additional, Companie, Alessandro, additional, Ratangee, Frances, additional, Veldtsman, Helen, additional, Petersen, Christel, additional, Abrahams, Charmaine, additional, Moses, Miriam, additional, Kelepu, Xoliswa, additional, Gregg, Yolande, additional, Swanepoel, Liticia, additional, Magawu, Nomsitho, additional, Cetywayo, Nompumelelo, additional, Mactavie, Lauren, additional, Valley, Habibullah, additional, Filander, Elizabeth, additional, Nqakala, Nambitha, additional, Maasdorp, Elizna, additional, Khoury, Justine, additional, Kriel, Belinda, additional, Smith, Bronwyn, additional, Muller, Liesel, additional, Tonsing, Susanne, additional, Loxton, Andre, additional, Hiemstra, Andriette, additional, Ahlers, Petri, additional, Flinn, Marika, additional, Chung, Eva, additional, Chung, Michelle, additional, and Sato, Alicia, additional

Published
2021
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