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MBA Professional Report The purpose of this MBA Project is to investigate and provide a comprehensive assessment for standardizing the ammunition distribution system used by the United States Navy. This project was conducted with the sponsorship and assistance of the Naval Supply Systems Command Global Logistics Support. The goal of this project was to identify and document both the push and pull methods of supply chain distribution and then recommend one of these methods, or possibly a hybrid of the two, to Naval Supply Systems Command for potential implementation. Analysis was performed on requisition information from the eight major Naval Weapons Stations to end users (those ordering particular types of ammunition) in an effort to determine which potential method would have the greatest economic impact for cost savings. http://archive.org/details/standardizingamm109457405 Lieutenant Commander, United States Navy,Lieutenant, United States Navy Approved for public release; distribution is unlimited.

Background The zebrafish Danio rerio is an important model system for drug discovery and to study cardiovascular development. Using a laser-scanning confocal microscope, we have developed a non-invasive method of measuring cardiac performance in zebrafish embryos and larvae that obtains cardiovascular parameters similar to those obtained using Doppler echocardiography in mammals. A laser scan line placed parallel to the path of blood in the dorsal aorta measures blood cell velocity, from which cardiac output and indices of vascular resistance and contractility are calculated. Results This technique, called laser-scanning velocimetry, was used to quantify the effects of pharmacological, developmental, and genetic modifiers of cardiac function. Laser-scanning velocimetry was applied to analyze the cardiovascular effects of morpholino knockdown of osmosensing scaffold for MEKK3 (OSM), which when mutated causes the human vascular disease cerebral cavernous malformations. OSM-deficient embryos had a constricted aortic arch and markedly increased peak cell velocity, a characteristic indicator of aortic stenosis. Conclusion These data validate laser-scanning velocimetry as a quantitative tool to measure cardiovascular performance for pharmacological and genetic analysis in zebrafish, which requires no specialized equipment other than a laser-scanning confocal microscope.