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Introduction: Problems related to patient safety in the pre-hospital context are little explored, but essential, given the vulnerability to incidents. Objective: To analyze the patient safety climate from the perspective of the multidisciplinary team that works in Mobile Pre-Hospital Care (PHC). Materials and Methods: Cross-sectional study, conducted in a Mobile Pre-Hospital Care. Data collection was performed through Safety attitudes Questionnaire (SAQ), with convenience sampling and participation rate of 94.3% of eligible professionals. Descriptive statistics and the Mann-Whitney test were used for data analysis. Results: Among the 151 participating professionals, males predominated (54.6%), nursing assistants and technicians (42.0%), working for 10 or more years (61.0%), in adult and pediatric care (93.4%). The SAQ Total had a median of 70, indicating a negative perception of the participants about patient safety in the APH. The domains with a negative perception were: Safety Climate, Stress Recognition, Management Perception and Working Conditions; while the Teamwork Climate and Job Satisfaction domains showed a positive perception. In the comparative analysis among professionals, differences were observed between some categories for the domains of Job Satisfaction, Stress Recognition and Working Conditions. Conclusions: This study presents the peculiarities of emergency medical services and the need to sensitize professionals and managers about patient safety, with a view to better understanding the current scenario and possibilities of reducing adverse events to improve the care offered. [ABSTRACT FROM AUTHOR]