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It is known that a number of difficulties arise in the development of oil fields characterized by non-Newtonian properties. Therefore, the study of the relaxation properties of a fluid that saturates a porous medium is an important task, which in the future will effectively control the development and production of such oils. The paper proposes a technique for studying relaxation properties by the method of comprehensive compression and revealing the dependence of relaxation properties on various parameters

To assess the feasibility of operating the well stock in various ways, it is necessary to take into account both the economic efficiency of their work and the presence of a hydrodynamic connection between them. The technical and economic profitability limit of well operation is the level of costs, taking into account energy-resource, technical potential, above which operation is inappropriate in today's market conditions. In the process of developing and operating oil fields, one often has to deal with unforeseen conditions and the impossibility of fully formalizing (justifying) the decision to select and carry out the necessary geological and technical measures. Taking into account the above, the authors propose an approach for processing array of field data based on statistical methods. This approach will help to make a prompt and substantiated decision on the management of the existing well stock.

The widespread introduction of the gas-lift method of extracting hydrocarbons is associated with the choice of the optimal well operation mode, the purpose of which is to save the working agent, improve the operation of the lift through the implementation of various technological measures, which makes the issue more and more relevant. In addition, the efficiency of field development requires taking into account the degree and nature of the interaction between wells. Important is the local nature of the studied technological modes of individual wells, which does not reflect the integral state of the "reservoir-well" system as a whole and, as a result, leads to instability of technological modes [1].

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© 2022, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.SUMMARY. Ovarian ischemia-reperfusion (I/R) injury develops due to oxidative stress and inflammation that occurs when tissue is deprived of oxygen. Ovarian I/R damage also causes infertility. The aim of this study is to see the effect of ovarian I/R damage on reproductive functions and to determine the protection of antioxidant and anti-inflammatory cerebrolysin in possible damage. Albino Wistar-type female rats were divided into sham operation (SG), ischemia-reperfusion (IR) and cerebrolysin+ischemia-reperfusion (CIR) groups. After I/R application with cerebrolysin, the ovaries of six animals from each group were taken and biochemical and histopathological examinations were performed. Six animals from each remaining group were left to breed. Our test results showed that cerebrolysin decreased I/R-related oxidant, proinflammatory cytokine and tissue damage and increased antioxidant levels. In addition, cerebrolysis significantly inhibited the delay of the maternity period. These findings indicate that cerebrolysin may be helpful in the treatment of I/R-related ovarian damage and infertility.

Potentilla species belong the family Rosaceae which have been used in precious medicinal, phytochemical and pharmacological studies. In current study, we aimed to determined antioxidant capacity, total secondary metabolites and phenolic composition of acetone and methanol extracts of Potentilla davisii. To evaluate antioxidant activity of extracts of P. davisii was used different five methods (P-carotene-linoleic acid assay, DPPH and ABTS radical scavenging activity, Phosphomolybdenum assay, CUPRAC assay). Total phenolic, flavonoid and tannin content were determined using Folin-Ciocalteau colorimetric method, Aliminum chlorid method and Sulphiric acid-vanillin method separately. Characterisation of methanol extract of P. davisii was analysed by HPLC method with 15 standards. The results showed that acetone extracts have highest total antioxidant activity, while methanol extracts have the highest radical scavenging activity and reducing power activity. Also, acetone extracts possess more total flavonoid and tannin amount. HPLC analysis indicated that caffeic acids is found as most common phenolic acid in methanol extract of P. davisii. According to these results, extracts of P. davisii could be natural product for medicinal, biological and industrial studies. © 2021 Parlar Scientific Publications. All rights reserved.

Phytochemical screening ofCalicotome villosaethanolic extracts in respect of phenolic compounds (HPLC method), antioxidant activity (DPPH and beta-carotene tests), determination of total phenolic and total flavonoid contents, and evaluation of cytotoxic (againstArtemia salina), larvicidal (againstCulex pipiensandMusca domestica) and anthelmintic activity (againstTubifex tubifex) have been performed. The flower extract exhibited higher biological activity than the stem extracts in all assays (DPPH, 0.6 mg/mL, IC50, beta-carotene, 75.12 +/- 0.73 %). There was good correlation between the antioxidant activity and total phenolic and total flavonoid contents. The flower extract exhibited significant cytotoxic activity (againstA. salina) with 0.312 mg/mL, LC(50)larvicidal activity (againstCx. pipiens) with 0.330 mg/mL, LC(50)and anthelmintic activity (againstT. tubifex) with 1.32 mg/mL, LC50. HPLC analysis showed that vanillic acid was major component in the flower extract. In conclusion,C. villosahas good biological activity for further studies in agriculture, medicine and pesticide industry. C1 [Turan, Murat] Pamukkale Univ, Fac Art & Sci, Dept Biol, Denizli, Turkey. [Mammadov, Ramazan] Mugla Sitki Kocman Univ, Fac Sci, Dept Mol Biol & Genet, Mugla, Turkey.

This work was designed to phenolic compound analysis with UPLC-ESI-MS/MS (Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry), antioxidant and larvicidal (against Musca domestica and Culex pipiens) activities of acetone, methanol and water extracts of different parts (fresh and underground) of G. bohémica extract. The antioxidant activities were determined using six complementary methods (DPPH, ABTS, Phosphomolybdenum, FRAP, CUPRAC assays and ß-Carotene-linoleic acid method). Among extracts, the lowest IC50 value in the DPPH test was obtained from the acetone extract of the underground part (0.652 ± 0.023 jj,g/mL, IC5 0).The determination of contents were evaluated using total phenolics, flavonoid and tannin contents and found maximum values in acetone extract of underground part 6.365 ± 0.064 (mgGAE/g extract), 27.634 ± 0.610 (mg CE/g extract), 22.968 ± 0.073 (mg CE/g extract) respectively. Phenolic compounds of water extracts were determined 32 phenolic compounds using UPLC-ESI-MS/MS, and caffeic acid for both parts was found as the major compound with a high amount. The fresh part is more toxic than the underground part with 60.212 ± 2.778 (1 mg/mL dose, %) against M. domestica, and with 0.190 (mg/mL, LC5 0) against Cx. pipiens. These results about G. bohemica was shown as a source of natural potential antioxidant, and have potential use of pharmaceutical, agricultural industry. © by PSP.

Plant derived products are widely used in cancer treatment. Gall species has been preferred for treatment various diseases in folk medicine, but there are few studies on the anticancer effects of gall species. The present study reports the antioxidant activity and total secondary metabolites of extracts ofA. tomentosusgalls. The antioxidant potency of galls was carried out using differentin-vitromodel systems. Their cytotoxic efficacy on Mia-Paca cell line was also explored. Gall extract was found to contain a large amount of phenolic acids. The extract potently scavenged free radicals including DPPH (IC(50)9.56 +/- 1.08 mu g/mL), ABTS (IC(50)18.51 +/- 0.25 mu g/mL). It can be seen as a potential source of antioxidants according to beta-carotene/linoleic acid method (%92.58 +/- 0.92) and Phosphomolybdenum assays (104.36 +/- 4.95 mgAE/g). Gall extract also posses ability of metal chelating (%40.07 +/- 2.30) and Fe3+(184.01 +/- 2.83 mgTE/g) and Cu2+(89.81 +/- 0.96 mgTE/g) reducing activity. According to total secondary metabolites tests, gall extract showed high total phenolic, total flavonoid and total tannin amount. HPLC analysis of the extract suggested it to contain caffeic acid (424.068.479 mu g/g), ellagic acid (187.696.132 mu g/g). XTT assay revealed gall extract to enhance percent survival of Mia-Paca2 cell line exposedA. tomentosusextracts. The best cytotoxic effect was determined in acetone extracts (IC50: 124.7 mu M). Expression of some genes (Bax, Bcl-2, FAS, BID, caspase-3, caspase-8, caspase-9, caspase-10, FADD, TRADD) in the apoptosis pathway was determined to invastigate apoptosis inducing activity. These results indicate thatA. tomentosusgalls possess potent antioxidant activity, when tested both in chemical as well as biological models. C1 [Kilincarslan Aksoy, Ozge] Pamukkale Univ, Dept Biol, Sci & Art Fac, Denizli, Turkey. [Mammadov, Ramazan] Mugla Sitki Kocman Univ, Dept Mol Biol Genet, Mugla, Turkey. [Secme, Mucahit] TurkeyPamukkale Univ, Med Fac, Dept Med Biol, Denizli, Turkey.

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (beta-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as beta-carotene/linoleic acid (%58.5 +/- 0.021), DPPH (IC50: 1.220 +/- 0.025) and ABTS (IC50:1.552 +/- 0.021), ferric reducing antioxidant power (FRAP) (4.151 +/- 0.056 mgTE/g), phoshomolibdenum assay (8.218 +/- 0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41 +/- 0.007 mgGAE/mL), (87.142 +/- 0.009 mu QE/g), and (30.178 +/- 0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 mu g/g) and 2,5dihidroksi (2544.96 mu g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents. C1 [Aydin, Cigdem; Rakhimzhanova, Akgul; Kilincarslan, Ozge; Mammadov, Ramazan] Pamukkale Univ, Sci Fac, Dept Biol, Denizli, Turkey.

The present study was designed to examine the total phenolic, flavonoid, tannin contents, the antioxidant, ALT/ALP enzyme and cytotoxic activities of the several extracts (ethanol, methanol, acetone and water) of L. martagon which is investigated for the flora of Turkey and Kazakhstan. The antioxidant activities of these extracts were analyzed by means of cleaning methods (DPPH and ABTS scavenging activity), P-carotene/linoleic acid test system, phosphonolibden method and metal chelating activity. Among the four different L. martagon extracts, the acetone extract showed the highest amount of radical scavenging both underground and aerial part in Turkey (DPPH, 245.82±1.59mg/mL and ABTS, 147.42±1.93mg/mL with IC50 values) and methanol extract antioxidant activities (P-carotene, 99.28%). In metal chelating activity, ethanol extract was different from other extracts. According to phosphonolybdenum method, the antioxidant capacity of the extracts ranged from 27.54 to 99.49mg/mL. The phenolic content of the ethanol, methanol and acetone extracts were analyzed using HPLC and some major phenolics were determined as: caffeic acid, cinnamic acid, epicatechin and ellagic. As a result of the study, ethanol (bulb 35.457 LC5 0 ) extract of the L. martagon exhibited highest cytotoxic activity whilst acetone (aerial part 213.596 LC5 0 ) exhibited lowest cytotoxic activity. ©by PSP.

The present study was designed to examine the total phenolic, flavonoid, tannin contents, the antioxidant, ALT/ALP enzyme and cytotoxic activities of the several extracts (ethanol, methanol, acetone and water) of L. martagon which is investigated for the flora of Turkey and Kazakhstan. The antioxidant activities of these extracts were analyzed by means of cleaning methods (DPPH and ABTS scavenging activity), beta-carotene/linoleic acid test system, phosphonolibden method and metal chelating activity. Among the four different L. martagon extracts, the acetone extract showed the highest amount of radical scavenging both underground and aerial part in Turkey (DPPH, 245.82 +/- 1.59mg/mL and ABTS, 147.42 +/- 1.93mg/mL with IC50 values) and methanol extract antioxidant activities (beta-carotene, 99.28%). In metal chelating activity, ethanol extract was different from other extracts. According to phosphonolybdenum method, the antioxidant capacity of the extracts ranged from 27.54 to 99.49mg/mL. The phenolic content of the ethanol, methanol and acetone extracts were analyzed using HPLC and some major phenolics were determined as: caffeic acid, cinnamic acid, epicatechin and ellagic. As a result of the study, ethanol (bulb 35.457 LC50) extract of the L. martagon exhibited highest cytotoxic activity whilst acetone (aerial part 213.596 LC50) exhibited lowest cytotoxic activity. C1 [Rakhimzhanova, Akgul; Mammadov, Ramazan] Pamukkale Univ, Fac Arts & Sci, Dept Biol, TR-20070 Denizli, Turkey.

Cyclamen L., belonging to the Primulaceae family, is a tuberous perennial geophyte with some taxa indigenous to Turkey. However, this genus has been poorly investigated for its cytotoxic and anticancer potentials. The current study aimed to explore the antiproliferative effects of the ethanolic extracts of three Cyclamen taxa (C. pseudibericum, C. mirabile and C. persicum) and their nitric oxide (NO) inhibitory activity in LPS-stimulated non-small cell lung cancer (NSCLC) cell lines, namely HCC78 and H1975. Also, total saponin contents of the extracts were determined as quillaja equivalents. The cytotoxicity of the Cyclamen extracts was assessed by the CellTiter-Glo assay. C. persicum extract caused a higher cytotoxic effect on both H1975 and HCC78 cells than the other two Cyclamen extracts and its IC50 values in H1975 and HCC78 cells were determined to be 17.27 and 34.15 ?g/ mL, respectively. While Griess reaction was performed to determine the nitrite levels as an index of NO production in LPS-stimulated NSCLC cells treated with the Cyclamen extracts, vanillin-sulphuric acid method was used to detect total saponin contents in the extracts. Among the three Cyclamen taxa evaluated, the highest inhibitory activity towards NO production in HCC78 cells was obtained with from C. pseudibericum, while C. persicum showed the highest inhibitory activity in H1975 cells. As a result, this study demonstrated that the tuber extracts of three Cyclamen taxa, which have been determined their total saponin contents, had significant cytotoxic activity and NO inhibitory potentials against HCC78 and H1975 non-small cell lung cancer cell lines. These data suggest that Cyclamen L. extracts examined in this study merit further research so as to isolate the bioactive secondary metabolites with anti-tumor potentials. © 2020 DSR Publishers/The University of Jordan. All Rights Reserved.

This work was designed to evaluate the phenolic compounds and biological activities (antioxidant, cytotoxic and anthelmintic) of Teucrium sandrasicum extracts (ethanol and acetone) as well as to determine proximate parameters (such as proteins, carbohydrates, fat). The phenolic contents were identified using HPLC. The ethanol extracts exhibited higher free radical scavenging and antioxidant activities than acetone extracts. The reducing power, metal chelating and radical cation activities were found to be statistically different between the acetone and ethanol extracts. T. sandrasicum exhibited cytotoxic, anthelmintic activities with rich nutrient contents. Based on these results, this plant may be considered as a potentially useful source for the food and pharmaceutical industry. C1 [Kaska, A.] Pamukkale Univ, Fac Educ, Dept Sci & Math, TR-20100 Denizli, Turkey. [Mammadov, R.] Pamukkale Univ, Fac Arts & Sci, Dept Biol, TR-20100 Denizli, Turkey.

Coriander (Coriandrum sativum L.) is such an herb from the Apiaceae family, used both for its medicinal and nutritional properties for many centuries. In this study, the effects of C. sativum extract on gene expression, viability, colony formation, migration, and invasion of PC-3 and LNCaP prostate cancer cell lines have been investigated. The half maximal inhibitory concentration (IC50) dose in PC-3 and LNCaP cells was detected to be 2 and 5 mg/mL at the 24th hour, respectively. C. sativum extracts have been observed to cause a significant decrease in the expression of Akt and Bcl-2 in the PC-3 cells and just Akt in LNCaP cells while increasing in the expression of p53, caspase-9, caspase-10, PTEN, DR5, TRADD, PUMA, and NOXA. DR4 expression was increased in LNCaP cell line but not PC-3, and APAF and BID had increased expression in PC-3 but not the LNCaP cells. Our observations have shown that C. sativum extract decreased colony formation while inhibiting cell invasion and migration. Cell migration was hindered in PC-3 but not the LNCaP cells. In conclusion, this data present a valuable addition to the very limited data available out there on the potential use of C. sativum in prostate cancer treatment. C1 [Elmas, Levent; Secme, Mucahit; Dodurga, Yavuz] Pamukkale Univ, Dept Med Biol, Fac Med, TR-20160 Denizli, Turkey. [Mammadov, Ramazan] Pamukkale Univ, Fac Arts & Sci, Dept Biol, Denizli, Turkey. [Fahrioglu, Umut] Near East Univ, Dept Med Biol, Fac Med, Nicosia, Cyprus.

This work was designed to evaluate the phenolic, flavonoid content and biological activities (antioxidant and cytotoxic) of E. tournefortii extracts (methanol, acetone and water) as well as to determined proximate parameters (ash, fat, protein and carbohydrate). Among the three different extracts of E. tournefortii evaluated, the methanol extract, showed the highest amount of antioxidant activities (beta-carotene, 88.62%). There were statistical differences among the radical scavenging (DPPH and ABTS) and antioxidant (phosphomolybdenum) activities of the different extracts of E. tournefortii. In the metal chelating and reducing power activities, the water extract exhibited the highest chelating and reducing capacity (29.88% and 0.206mg/mL respectively). All extracts of E. tournefortii exhibited cytotoxic activities and this plant possesses nutrients. These findings will provide addition information for the further investigation of this plant, for understanding the efficacy of E. tournefortii as a food ingredient, as well as for preventing oxidative stress mediated disorders. C1 [Kaska, Arzu] Pamukkale Univ, Dept Sci & Math, Fac Educ, Denizli, Turkey. [Mammadov, Ramazan] Pamukkale Univ, Fac Arts & Sci, Dept Biol, Denizli, Turkey.

Lamiaceae plants are widely used in traditional medicine as a cure for various disorders. Nepeta italica L. subsp. cadmea (Boiss.) A.L. Budantsev and Teucrium sandrasicum O. Schwarz are medicinal species belonging to the Lamiaceae family and are endemic to Turkey. The present study was designed to investigate the biological activities (antioxidant and toxicity) and phenolic content of these plants' extracts as well as to determine their mineral elements. The antioxidant properties of these plants were determined using the scavenging methods (DPPH and ABTS), total antioxidant test systems (beta-carotene/linoleic acid and phosphomolybdenum), metal chelating and reducing power assays. The phenolics were identified using HPLC and the brine shrimp lethality test was used to screen for possible toxicity. In addition, an evaluation was made of some mineral elements (P, Mg, K, Fe and Cu). The hydroethanolic extract of N. italica subsp. cadmea, showed the highest amount of antioxidant activity (beta-carotene, similar to 93%) among the extracts that were investigated. All the extracts exhibited chelating and reducing activities. There were statistical differences among the scavenging activities of the N. cadmea and T. sandrasicum extracts. The total antioxidant capacity of the extracts evaluated with the phosphomolybdenum test was in the range of 61.54-143.97 mu g/mg and that these plants possess mineral elements. These types of investigations are important for gainingmore information about the potential natural antioxidants from various Lamiaceae plants. The findings of the present study will provide additional information for understanding the efficacy of these medicinal plants as a food ingredient, as well as for use in medicinal applications. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved. C1 [Kaska, A.] Pamukkale Univ, Fac Educ, Dept Sci & Math, Denizli, Turkey. [Cicek, M.; Mammadov, R.] Pamukkale Univ, Fac Arts & Sci, Dept Biol, Denizli, Turkey.

Dried bulbs and leaves of Allium reuterianum were extracted with different solvents and evaluated for antioxidant, antibacterial, insecticidal, in vitro cytotoxic activities. The obtained results indicated that the highest compound 3, 4-dihidroksi benzoic acid with 166.2 mu g/g in extracts. The ethanolic extracts had the highest phenolic content and acetone extracts had the lowest fenolic content. In the beta-carotene-linoleic acid test system, bulb methanol (ABM) and leaf methanol (ALM) extracts showed the highest antioxidant activity. The mean antioxidant activity of ABM and ALM were 75.76 +/- 1.22% and 73.42 +/- 1.03%, respectively. The highest ferric-reducing power of extract (ABM) was determined 12.75 +/- 0.010 trolox equivalent (mg/g). Methanolic extract (ALM) exhibited a dose-dependent scavenging of DPPH and ABTS radicals with IC50 values of 0.512 +/- 0.003 mg/mL and 0.378 +/- 0.002 mg/mL respectively. Very strong reduction of gram-positive Staphylococcus aureus growth were observed during incubation of bacteria in bulb extracts (MIC was 20 +/- 1.01 mu g/mL). The brine shrimp lethality assay of bulb extract has showed good toxic to brine shrimp nauplii, with LC50 of 3.987 mu g/mL. The bulb extract of A. reuterianum showed highest larvicidal activity against Cx. pipiens with value LC50 (6.4129 mu g/mL). The results suggest that these plants could be used as a source of natural antioxidant and antibacterial agents. C1 [Aydin, Cigdem; Mammadov, Ramazan] Pamukkale Univ, Fac Sci, Dept Biol, Denizli, Turkey.

The focus of the experiment described in this paper has been to study the influence of cadmium on the morphophysiological and biochemical characteristics of buckwheat sprouts of various ploidity and to investigate stress responses of plants at early, physiologically sensitive stages of development. It has been established that growth of the aboveground plant organs and the root system of diploid buckwheat was more inhibited by cadmium compared to tetraploid buckwheat sprouts. Cadmium uptake by the aboveground parts of plants was insignificant and was not influenced by the ploidity level, whereas the root systems of tetraploid plants demonstrated an increased ability to accumulate cadmium compared to the roots of diploid buckwheat. According to the results of biochemical research of the buckwheat sprouts' ability to accumulate phenolic compounds of different classes and to produce phenylalanine ammonia lyase (PAL), the basic plant enzyme that catalyses phenolic biosynthesis, the tetraploid buckwheat species demonstrated an increase in all tested characteristics unlike the diploid form which showed a drop in the polyphenols content when PAL activity went up. The obtained results are relevant for further studies of phenolic biosynthesis in plant species of various ploidity levels and identifying the role of the key enzyme involved in phenolic compound metabolism that activates the phenylpropanoid pathway and can, in the case of increased cadmium exposure, be involved in the synthesis of secondary metabolites that are different from phenolic compounds. C1 [Kazantseva, Varvara; Goncharuk, Evgenia; Kartashov, Alexander; Zagoskina, Natalia] IPP RAS, KA Timiryazev Inst Plant Physiol RAS, 35 Bot Skaya St, Moscow 127276, Russia. [Mammadov, Ramazan] Pamukkale Univ, Fac Sci & Art, Dept Biol, TR-20017 Denizli, Turkey.

BackgroundThere is increasing interest in the pharmaceutical and food industries to substitute synthetic chemicals with naturally occurring compounds possessing bioactive properties. Plants are valuable sources of bioactive compounds. The present study investigates the chemical composition and antioxidant, antimicrobial, and anticancer activities of ethanolic extracts (EEs) and essential oils (EOs) from two species in the Lamiaceae family, Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut., cultivated in the Algerian Saharan Atlas.MethodsThe total flavonoid contents of the plants' ethanolic extracts were determined by the aluminium chloride method, while the total phenols were determined using the Folin-Ciocalteu method. Essential oils were obtained by hydrodistillation of the aerial parts of the plants and were analysed by GC-MS. The free radical-scavenging ability and antioxidant potential of the plants' EEs and EOs were probed using the 2, 2-diphenyl-picrylhydrazyl radical-scavenging, ABTS radical-scavenging, ferric-reducing power and phosphomolybdenum assays. The antimicrobial activities were evaluated against several pathogens characteristic of gram-negative bacteria (three species), gram-positive bacteria (three species) and fungi (two species). The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). The oils' anticancer potential against several cancer types was also studied using the MTT assay and reported as the toxic doses that resulted in a 50% reduction in cancer cell growth (LD50).ResultsPhenolic compounds in the EEs from both plants were analysed by HPLC and demonstrated a rich flavonoid content. Chemical analysis of the essential oil from Ocimum basilicum revealed 26 unique compounds, with linalool (52.1%) and linalyl acetate (19.1%) as the major compounds. A total of 29 compounds were identified in the essential oil from Thymus algeriensis, with -terpinyl acetate (47.4%), neryl acetate (9.6%), and -pinene (6.8%) as the major compounds. The ethanolic extracts and essential oils from both plants exhibited moderate antioxidant activities and moderate to weak antimicrobial activities. Furthermore, anticancer activities against the examined human cancer cell lines were associated with only the EOs from both plants, with LD50 values ranging between 300 and 1000g/mL.ConclusionThe results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies. C1 [Rezzoug, Maria; Bakchiche, Boulanouar; Gherib, Abdelaziz] Laghouat Univ, Fac Technol, Lab Proc Engn, Laghouat 03000, Algeria. [Roberta, Ascrizzi; Guido, Flamini] Univ Pisa, Dipartimento Farm, Via Bonanno 6, I-56126 Pisa, Italy. [Kilincarslan, Ozge; Mammadov, Ramazan] Pamukkale Univ, Fac Sci & Arts, Dept Biol, Denizli, Turkey. [Bardaweel, Sanaa K.] Univ Jordan, Sch Pharm, Dept Pharmaceut Sci, Queen Rania St, Amman 11942, Jordan.

Scientists are increasingly focusing attention on natural products of plant origin for use as agents in cancer protection and treatment. Cyclamen L. tuber extracts contain saponin glycosides that have been shown to have anti-cancer and other biological activities. The epithelial-to-mesenchymal transition (EMT) is thought to enhance malignant tumour progress. The transcriptional repressor zinc-finger E-box binding homeobox 1 (ZEB1) is an important inducer of EMT in different human tumours and has recently been shown to boost invasion by tumour cells. In this study, we investigated the effects of endemic Cyclamen pseudibericum (CP) saponin-rich tuber extract on the capacity of non-small cell lung cancer line A549 cells to proliferate, invade and migrate and also examined the expression levels of several invasion-migration-related microRNAs (miRNAs) to identify those which directly targeted ZEB1. The cytotoxicity effect of the CP extract on the A549 cancer cells was determined by the luminometric method. The half-minimal (50%) inhibitory concentration dose in the A549 cells was determined to be 41.64 ± 2.35 µg/mL. Using the Matrigel invasion chamber system and the wound healing assay we observed that the CP extract suppressed the invasion and migration capacity of A549 cells, respectively. The expression of miRNAs in A549 cells was evaluated by real-time PCR. Our data showed that overexpression of miRNA miR-200c hindered the EMT by increasing the expression of E-cadherin and decreasing the expression of both N-cadherin and vimentin through the direct targeting of ZEB1. These findings suggest that the saponin-rich tuber extract of CP may have considerable anti-cancer properties in lung cancer. Further studies are required to examine in detail the molecular-based mechanism involved in the EMT process of the extract along with isolation and identification of active saponin components.

Scientists are increasingly focusing attention on natural products of plant origin for use as agents in cancer protection and treatment. Cyclamen L. tuber extracts contain saponin glycosides that have been shown to have anti-cancer and other biological activities. The epithelial-to-mesenchymal transition (EMT) is thought to enhance malignant tumour progress. The transcriptional repressor zinc-finger E-box binding homeobox 1 (ZEB1) is an important inducer of EMT in different human tumours and has recently been shown to boost invasion by tumour cells. In this study, we investigated the effects of endemic Cyclamen pseudibericum (CP) saponin-rich tuber extract on the capacity of non-small cell lung cancer line A549 cells to proliferate, invade and migrate and also examined the expression levels of several invasion-migration-related microRNAs (miRNAs) to identify those which directly targeted ZEB1. The cytotoxicity effect of the CP extract on the A549 cancer cells was determined by the luminometric method. The half-minimal (50%) inhibitory concentration dose in the A549 cells was determined to be 41.64 +/- 2.35 A mu g/mL. Using the Matrigel invasion chamber system and the wound healing assay we observed that the CP extract suppressed the invasion and migration capacity of A549 cells, respectively. The expression of miRNAs in A549 cells was evaluated by real-time PCR. Our data showed that overexpression of miRNA miR-200c hindered the EMT by increasing the expression of E-cadherin and decreasing the expression of both N-cadherin and vimentin through the direct targeting of ZEB1. These findings suggest that the saponin-rich tuber extract of CP may have considerable anti-cancer properties in lung cancer. Further studies are required to examine in detail the molecular-based mechanism involved in the EMT process of the extract along with isolation and identification of active saponin components.

Erysimum is the second richest genus in Brassicaceae. Erysimum species have been used in some phytochemical and biological activities studies, as well as in the taxonomic, floristic and genetic works. In this study, some biological activities and phenolic contents of various solvents extracts (ethanol, acetone and distileted water) of Erysimum kotschyanum Gay. were evaluated..The antioxidant activity of the extracts was evaluated by 13-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2'-azinobis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS) and total phenolic and flavonoid contents in the extracts were determined. The brine shrimp lethality test was used to determine the cytotoxic activity of E. kotschyanum extracts and also, antibacterial potential of extracts obtained. Antioxidant activity was determined as 13-carotene/linoleic acid (%80.47 +/- 1.83), DPPH (IC50: 0.29 +/- 0.03) and ABTS (IC50: 47.15 +/- 0.74), ferric reducing antioxidant power (FRAP) (IC50: 18.79 +/- 0.15), total phenolic and total flavonoid contents was found as (4.883 +/- 0.47 mgGAE/g) and (93.322 +/- 1.57 mgQE/g), respectively ,and then 9 different phenolic compounds in ethanol extracts was carried out by HPLC. Also, ethanol extract's antimicrobial activity (17 +/- 1 zone diameter) and cytotoxic activity (Brine Shrimp LC50: 315.48) of extracts was calculated. According the results, the extract of E. kotschyanum may be considered as a potential source of biological agents.

Медицинская биофизика

Медицинская биофизика

Nepeta cadmea Boiss. is a species endemic to Turkey that belongs to the Nepeta genus. Several species of this genus are used in folk medicine. This study was designed to investigate the phenolic compounds, antioxidant, anthelmintic, and cytotoxic activities of various extracts (ethanol, methanol, acetone, and water) of N. cadmea. The antioxidant activities of these extracts were analyzed using scavenging methods (DPPH, ABTS, and H2O2 scavenging activity), the β-carotene/linoleic acid test system, the phosphomolybdenum method, and metal chelating activity. Among the 4 different extracts of N. cadmea that were evaluated, the water extract showed the highest amount of radical scavenging (DPPH, 25.54 μg/mL and ABTS, 14.51 μg/mL) and antioxidant activities (β-carotene, 86.91%). In the metal chelating and H2O2 scavenging activities, the acetone extract was statistically different from the other extracts. For the phosphomolybdenum method, the antioxidant capacity of the extracts was in the range of 8.15 to 80.40 μg/mg. The phenolic content of the ethanol extract was examined using HPLC and determined some phenolics: epicatechin, chlorogenic, and caffeic acids. With regard to the anthelmintic properties, dose-dependent activity was observed in each of the extracts of N. cadmea. All the extracts exhibited high cytotoxic activities. The results will provide additional information for further studies on the biological activities of N. cadmea, while also helping us to understand the importance of this species. Furthermore, based on the results obtained, N. cadmea may be considered as a potentially useful supplement for the human diet, as well as a natural antioxidant for medicinal applications. Practical Application: The plants of the Nepeta genus have been extensively used as traditional herbal medicines. Nepeta cadmea Boiss., one of the species belonging to the Nepeta genus, is a species endemic to Turkey. In our study, we demonstrated the antioxidant capacities, total phenolic, flavonoid, tannin content, anthelmintic, and cytotoxic activities of various extracts of Nepeta cadmea. The present study could well supply valuable data for future investigations and further information on the potential use of this endemic plant for humans, in both dietary and pharmacological applications. © 2018 Institute of Food Technologists®

Nepeta cadmea Boiss. is a species endemic to Turkey that belongs to the Nepeta genus. Several species of this genus are used in folk medicine. This study was designed to investigate the phenolic compounds, antioxidant, anthelmintic, and cytotoxic activities of various extracts (ethanol, methanol, acetone, and water) of N. cadmea. The antioxidant activities of these extracts were analyzed using scavenging methods (DPPH, ABTS, and H2O2 scavenging activity), the -carotene/linoleic acid test system, the phosphomolybdenum method, and metal chelating activity. Among the 4 different extracts of N. cadmea that were evaluated, the water extract showed the highest amount of radical scavenging (DPPH, 25.54 g/mL and ABTS, 14.51 g/mL) and antioxidant activities (-carotene, 86.91%). In the metal chelating and H2O2 scavenging activities, the acetone extract was statistically different from the other extracts. For the phosphomolybdenum method, the antioxidant capacity of the extracts was in the range of 8.15 to 80.40 g/mg. The phenolic content of the ethanol extract was examined using HPLC and determined some phenolics: epicatechin, chlorogenic, and caffeic acids. With regard to the anthelmintic properties, dose-dependent activity was observed in each of the extracts of N. cadmea. All the extracts exhibited high cytotoxic activities. The results will provide additional information for further studies on the biological activities of N. cadmea, while also helping us to understand the importance of this species. Furthermore, based on the results obtained, N. cadmea may be considered as a potentially useful supplement for the human diet, as well as a natural antioxidant for medicinal applications. Practical ApplicationThe plants of the Nepeta genus have been extensively used as traditional herbal medicines. Nepeta cadmea Boiss., one of the species belonging to the Nepeta genus, is a species endemic to Turkey. In our study, we demonstrated the antioxidant capacities, total phenolic, flavonoid, tannin content, anthelmintic, and cytotoxic activities of various extracts of Nepeta cadmea. The present study could well supply valuable data for future investigations and further information on the potential use of this endemic plant for humans, in both dietary and pharmacological applications.